AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0181200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0181200 MtrunA17_Chr1g0203401 94.366 213 8 1 1 213 1 209 2.01e-139 388
Msa0181200 MtrunA17_Chr8g0349271 60.000 180 62 2 34 213 31 200 9.01e-66 201
Msa0181200 MtrunA17_Chr8g0386521 58.400 125 52 0 89 213 74 198 1.00e-47 155
Msa0181200 MtrunA17_Chr5g0397781 61.475 122 47 0 92 213 86 207 1.35e-46 152
Msa0181200 MtrunA17_Chr7g0247121 69.231 65 20 0 149 213 34 98 4.92e-26 96.7
Msa0181200 MtrunA17_Chr1g0173571 31.013 158 95 1 51 208 126 269 1.34e-14 70.9
Msa0181200 MtrunA17_Chr1g0153281 35.577 104 66 1 110 213 513 615 1.71e-12 65.9
Msa0181200 MtrunA17_Chr8g0376741 38.532 109 64 1 98 206 412 517 3.46e-12 64.7
Msa0181200 MtrunA17_Chr7g0257771 34.959 123 79 1 86 208 154 275 4.36e-12 63.9
Msa0181200 MtrunA17_Chr8g0377061 34.615 104 68 0 110 213 251 354 5.33e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0181200 AT3G48940.1 61.628 172 60 2 46 213 6 175 6.00e-66 201
Msa0181200 AT5G23750.2 70.667 150 38 2 65 213 57 201 6.82e-66 202
Msa0181200 AT5G23750.3 77.778 126 28 0 88 213 104 229 8.47e-65 200
Msa0181200 AT5G23750.1 77.778 126 28 0 88 213 77 202 1.04e-64 199
Msa0181200 AT3G61260.1 61.850 173 58 3 42 213 47 212 5.17e-64 197
Msa0181200 AT2G45820.1 64.935 154 52 1 60 213 39 190 2.13e-54 172
Msa0181200 AT1G63295.1 44.628 121 64 1 96 213 31 151 5.51e-22 88.2
Msa0181200 AT1G63295.2 46.364 110 59 0 96 205 71 180 8.83e-22 89.0
Msa0181200 AT4G00670.1 42.157 102 59 0 107 208 16 117 2.54e-20 83.2
Msa0181200 AT2G41870.1 33.884 121 79 1 94 213 153 273 4.55e-13 67.0
Msa0181200 AT3G57540.1 33.058 121 79 1 94 212 175 295 1.29e-12 65.9
Msa0181200 AT1G30320.1 39.167 120 66 2 98 213 392 508 3.11e-12 65.5
Msa0181200 AT1G67590.1 37.500 104 65 0 110 213 235 338 1.29e-11 63.2
Msa0181200 AT2G02170.1 42.308 104 60 0 110 213 379 482 6.08e-11 61.6
Msa0181200 AT2G02170.2 42.308 104 60 0 110 213 379 482 6.08e-11 61.6
Msa0181200 AT2G02170.5 42.308 104 60 0 110 213 379 482 6.08e-11 61.6
Msa0181200 AT2G02170.4 42.308 104 60 0 110 213 379 482 6.08e-11 61.6
Msa0181200 AT2G02170.3 42.308 104 60 0 110 213 254 357 6.69e-11 61.2
Msa0181200 AT2G02170.6 42.308 104 60 0 110 213 254 357 6.69e-11 61.2

Find 68 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGACCAAAGAGTTTCTT+TGG 0.217523 1_4:+69364953 None:intergenic
TCTTAGCTGGGTGGGTTTAT+TGG 0.252899 1_4:+69367180 None:intergenic
ATTACAAGTGTTGGAGAGAT+AGG 0.253847 1_4:+69367072 None:intergenic
TCCTCAGCTACATCTTTCTT+TGG 0.259226 1_4:+69366806 None:intergenic
TCGATAGAGCCGCCCTCTTT+TGG 0.297765 1_4:+69365634 None:intergenic
GGAACAACTAGAGAAGAAAA+AGG 0.300679 1_4:-69365122 Msa0181200:CDS
GTTGTATATATCCATTACTT+TGG 0.347191 1_4:-69364793 Msa0181200:three_prime_UTR
TCTTTCTAAGAAAGAAGAAT+TGG 0.355358 1_4:+69366970 None:intergenic
TTGTATATATCCATTACTTT+GGG 0.362237 1_4:-69364792 Msa0181200:three_prime_UTR
ACATCTTTCTTTGGAGCCTC+AGG 0.378728 1_4:+69366815 None:intergenic
ACTGTTCTCCCATGCTGTTA+TGG 0.379485 1_4:+69365271 None:intergenic
ATTCTACTAAAAGTCTTAGC+TGG 0.382428 1_4:+69367167 None:intergenic
GCCTCAGGGAGAGGAGGAGA+AGG 0.390077 1_4:+69366830 None:intergenic
ACAGCTCCAAAGAAACTCTT+TGG 0.398417 1_4:-69364959 Msa0181200:CDS
AGTTGGCACAGCGCACCAAT+AGG 0.402374 1_4:+69367217 None:intergenic
AAGAAAGATATTACAAGTGT+TGG 0.420015 1_4:+69367063 None:intergenic
AAGAGGTAGCTGCAAAATAC+AGG 0.425184 1_4:-69364991 Msa0181200:CDS
CTGAGCTATGACAAGAGCTT+TGG 0.429684 1_4:+69366724 None:intergenic
GCTGCAAAATACAGGGCAAC+TGG 0.430234 1_4:-69364983 Msa0181200:CDS
TTCTTTGGAGCCTCAGGGAG+AGG 0.442969 1_4:+69366821 None:intergenic
AATAAGTTGTCTGTCCCTAT+TGG 0.457363 1_4:-69367232 None:intergenic
AGCTGGGTGGGTTTATTGGT+AGG 0.476393 1_4:+69367184 None:intergenic
TCAGTTGAAGCAGGTGGTGG+TGG 0.488620 1_4:+69366869 None:intergenic
GCTTTGGAGTCATCAACAAC+AGG 0.488845 1_4:+69366740 None:intergenic
TCAACAACAGGAGGAGGAGG+AGG 0.496787 1_4:+69366752 None:intergenic
GTTGAAGCAGGTGGTGGTGG+AGG 0.497469 1_4:+69366872 None:intergenic
ACTCACTCATTCTTAGATGT+TGG 0.501732 1_4:-69364706 Msa0181200:three_prime_UTR
GTTGGCACAGCGCACCAATA+GGG 0.504102 1_4:+69367218 None:intergenic
TATTATAGTTGTAGAATGAA+AGG 0.511225 1_4:+69367106 None:intergenic
GAGTCAGTGGTCTAATACAA+AGG 0.513455 1_4:+69364725 None:intergenic
ATTATAGTTGTAGAATGAAA+GGG 0.515536 1_4:+69367107 None:intergenic
CAAGGCAGCTGAAGAAAAGA+AGG 0.521517 1_4:-69365056 Msa0181200:CDS
GGCTACAATGTTCAAGATTG+TGG 0.522501 1_4:-69364926 Msa0181200:three_prime_UTR
TCATCAACAACAGGAGGAGG+AGG 0.524276 1_4:+69366749 None:intergenic
TTCTACTAAAAGTCTTAGCT+GGG 0.524449 1_4:+69367168 None:intergenic
CCTCAGGGAGAGGAGGAGAA+GGG 0.530917 1_4:+69366831 None:intergenic
TGGTAGGTACTCTTGTGAGT+TGG 0.531008 1_4:+69367200 None:intergenic
GGCTGTCACTAATCAAAGCA+TGG 0.531510 1_4:-69365485 Msa0181200:CDS
CTCAGGGAGAGGAGGAGAAG+GGG 0.532056 1_4:+69366832 None:intergenic
TTACAAGTGTTGGAGAGATA+GGG 0.534965 1_4:+69367073 None:intergenic
GTCTCAGTTGAAGCAGGTGG+TGG 0.537604 1_4:+69366866 None:intergenic
GGAGGAGGATTATTATCTTG+TGG 0.541469 1_4:+69366770 None:intergenic
CAAGAGTTGCAACAGAAAAG+AGG 0.549164 1_4:-69365506 Msa0181200:CDS
ACTAAAAGTCTTAGCTGGGT+GGG 0.558329 1_4:+69367172 None:intergenic
TTTGGAGCCTCAGGGAGAGG+AGG 0.558769 1_4:+69366824 None:intergenic
AAGTGTTGGAGAGATAGGGT+TGG 0.563164 1_4:+69367077 None:intergenic
CATCTTTCTTTGGAGCCTCA+GGG 0.563937 1_4:+69366816 None:intergenic
TGCTGAAGAGAAACCAAAAG+AGG 0.570519 1_4:-69365647 Msa0181200:CDS
GAGTCATCAACAACAGGAGG+AGG 0.575285 1_4:+69366746 None:intergenic
AACTTTCATCCATAACAGCA+TGG 0.578010 1_4:-69365280 Msa0181200:CDS
TCCAAAGAAAGATGTAGCTG+AGG 0.578942 1_4:-69366807 Msa0181200:CDS
GCTATGATTGAGGCAAAGAA+AGG 0.579579 1_4:-69365034 Msa0181200:CDS
TACTAAAAGTCTTAGCTGGG+TGG 0.586125 1_4:+69367171 None:intergenic
AGAATTACTCAAAGCAGAAG+AGG 0.586549 1_4:-69365008 Msa0181200:CDS
CAAAATAGCAGCACTTCACA+AGG 0.592299 1_4:-69365074 Msa0181200:CDS
AGAAAAGAAGGCTATGATTG+AGG 0.593916 1_4:-69365044 Msa0181200:CDS
GTAGTCGTCTCAGTTGAAGC+AGG 0.595047 1_4:+69366860 None:intergenic
AGCTGAATTGAGAAAACTTG+AGG 0.599321 1_4:-69365231 Msa0181200:intron
GCTGTCACTAATCAAAGCAT+GGG 0.606143 1_4:-69365484 Msa0181200:CDS
ACTTTCATCCATAACAGCAT+GGG 0.613867 1_4:-69365279 Msa0181200:CDS
CCCTTCTCCTCCTCTCCCTG+AGG 0.617803 1_4:-69366831 Msa0181200:CDS
AGAGGTAGCTGCAAAATACA+GGG 0.626640 1_4:-69364990 Msa0181200:CDS
GTCGTCTCAGTTGAAGCAGG+TGG 0.640903 1_4:+69366863 None:intergenic
TCTAAGAATGAGTGAGTCAG+TGG 0.643025 1_4:+69364712 None:intergenic
GCTGAAGAGAAACCAAAAGA+GGG 0.649888 1_4:-69365646 Msa0181200:CDS
TTGGAGTCATCAACAACAGG+AGG 0.656015 1_4:+69366743 None:intergenic
GAAGAGAAACCAAAAGAGGG+CGG 0.689508 1_4:-69365643 Msa0181200:CDS
GAGGGCGGCTCTATCGACCG+TGG 0.735676 1_4:-69365628 Msa0181200:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTATTACTTTTAATAGA+AGG - chr1_4:69365527-69365546 Msa0181200:intron 10.0%
!!! AATTATTACTTTTAATAGAA+GGG - chr1_4:69365528-69365547 Msa0181200:intron 10.0%
!! TGATTTAAAAACAAATATTA+CGG + chr1_4:69366170-69366189 None:intergenic 10.0%
!! GTTGAAAAAAATCAATTATA+GGG + chr1_4:69365286-69365305 None:intergenic 15.0%
!!! ATCAATACATATTTTTTATC+TGG - chr1_4:69365334-69365353 Msa0181200:intron 15.0%
!!! TTTAAATTTGTGCATATTTT+TGG + chr1_4:69365993-69366012 None:intergenic 15.0%
!! ATATAATTCTGTTGTTAATT+TGG - chr1_4:69366220-69366239 Msa0181200:intron 15.0%
!! ATTATAGTTGTAGAATGAAA+GGG + chr1_4:69364810-69364829 None:intergenic 20.0%
!! TATTATAGTTGTAGAATGAA+AGG + chr1_4:69364811-69364830 None:intergenic 20.0%
!! CGTTGAAAAAAATCAATTAT+AGG + chr1_4:69365287-69365306 None:intergenic 20.0%
!! TAATAGCAAAATTGAATTGA+AGG - chr1_4:69365437-69365456 Msa0181200:intron 20.0%
!! TTAATTGATTAGATTGTAGT+TGG + chr1_4:69365837-69365856 None:intergenic 20.0%
!! AATCTAATCAATTAAGTGTA+TGG - chr1_4:69365842-69365861 Msa0181200:intron 20.0%
!!! AAAAAGGTAAAGGTTATTTT+GGG + chr1_4:69366126-69366145 None:intergenic 20.0%
!! TATATCAATGTGATTATATG+CGG + chr1_4:69366205-69366224 None:intergenic 20.0%
!!! TAATGCTTAATGTGTATTTT+TGG - chr1_4:69366502-69366521 Msa0181200:intron 20.0%
!! TTGTATATATCCATTACTTT+GGG - chr1_4:69367122-69367141 Msa0181200:five_prime_UTR 20.0%
! AAGAAAGATATTACAAGTGT+TGG + chr1_4:69364854-69364873 None:intergenic 25.0%
!!! AAGAATTGGAACAACTTTTT+TGG + chr1_4:69364933-69364952 None:intergenic 25.0%
!! TCTTTCTAAGAAAGAAGAAT+TGG + chr1_4:69364947-69364966 None:intergenic 25.0%
!!! GATTCAGATTCTATCTTTTT+TGG + chr1_4:69365012-69365031 None:intergenic 25.0%
! TTATAGGGAAAATTTGAAGA+TGG + chr1_4:69365271-69365290 None:intergenic 25.0%
! AAACATGCATTAAGATTCTA+GGG - chr1_4:69365942-69365961 Msa0181200:intron 25.0%
!! GAAAAAGGTAAAGGTTATTT+TGG + chr1_4:69366127-69366146 None:intergenic 25.0%
! CTAAAACATGAAAAAGGTAA+AGG + chr1_4:69366136-69366155 None:intergenic 25.0%
!!! CTTTACCTTTTTCATGTTTT+AGG - chr1_4:69366134-69366153 Msa0181200:intron 25.0%
! AAACAAATATTACGGATGAA+TGG + chr1_4:69366162-69366181 None:intergenic 25.0%
! AATACACATTAAGCATTAGA+TGG + chr1_4:69366500-69366519 None:intergenic 25.0%
! TACTAATCTCCTTAGAATTA+TGG + chr1_4:69366565-69366584 None:intergenic 25.0%
! ACATTGAATGTGACAAAATA+TGG - chr1_4:69366732-69366751 Msa0181200:CDS 25.0%
! CATTGAATGTGACAAAATAT+GGG - chr1_4:69366733-69366752 Msa0181200:CDS 25.0%
! ATTGAATGTGACAAAATATG+GGG - chr1_4:69366734-69366753 Msa0181200:CDS 25.0%
!!! AATTACTTTTCACTGTGAAT+AGG - chr1_4:69366771-69366790 Msa0181200:CDS 25.0%
! GTTGTATATATCCATTACTT+TGG - chr1_4:69367121-69367140 Msa0181200:five_prime_UTR 25.0%
!!! TGTTTTTATTCCCAAAGTAA+TGG + chr1_4:69367135-69367154 None:intergenic 25.0%
TTCTACTAAAAGTCTTAGCT+GGG + chr1_4:69364749-69364768 None:intergenic 30.0%
ATTCTACTAAAAGTCTTAGC+TGG + chr1_4:69364750-69364769 None:intergenic 30.0%
ATATAAGCGAAATAGTTGAG+AGG - chr1_4:69365377-69365396 Msa0181200:intron 30.0%
GGAAACTGAGAAAAATACAA+GGG + chr1_4:69365737-69365756 None:intergenic 30.0%
TGGAAACTGAGAAAAATACA+AGG + chr1_4:69365738-69365757 None:intergenic 30.0%
ATCAGAAAAATTCCAGCAAA+TGG + chr1_4:69365758-69365777 None:intergenic 30.0%
TTTCTGATGATTGTTCTCTT+AGG - chr1_4:69365770-69365789 Msa0181200:intron 30.0%
TTGATTAGATTGTAGTTGGA+TGG + chr1_4:69365833-69365852 None:intergenic 30.0%
CTAGAATCTTAATGCATGTT+TGG + chr1_4:69365943-69365962 None:intergenic 30.0%
CAAACATGCATTAAGATTCT+AGG - chr1_4:69365941-69365960 Msa0181200:intron 30.0%
AGAAAAGAAACACATTAGCT+TGG - chr1_4:69366012-69366031 Msa0181200:intron 30.0%
TGGTTCCTAAAACATGAAAA+AGG + chr1_4:69366142-69366161 None:intergenic 30.0%
CAATGACAAGATATATACCA+CGG + chr1_4:69366306-69366325 None:intergenic 30.0%
TAGCATATGAAGTGCATAAA+GGG + chr1_4:69366342-69366361 None:intergenic 30.0%
TTAGCATATGAAGTGCATAA+AGG + chr1_4:69366343-69366362 None:intergenic 30.0%
ACTTCATATGCTAACATTAC+AGG - chr1_4:69366349-69366368 Msa0181200:intron 30.0%
TATGGTGACTCAATGTAATA+AGG + chr1_4:69366547-69366566 None:intergenic 30.0%
ATTGAGTCACCATAATTCTA+AGG - chr1_4:69366553-69366572 Msa0181200:intron 30.0%
TTGAATGTGACAAAATATGG+GGG - chr1_4:69366735-69366754 Msa0181200:CDS 30.0%
GAACAACTAGAGAAGAAAAA+GGG - chr1_4:69366793-69366812 Msa0181200:CDS 30.0%
AAAAGACCAAAGAGTTTCTT+TGG + chr1_4:69366964-69366983 None:intergenic 30.0%
!! AAACTCTTTGGTCTTTTCTA+AGG - chr1_4:69366967-69366986 Msa0181200:five_prime_UTR 30.0%
! TGTTTTCTTCATGTGTGATT+TGG - chr1_4:69367043-69367062 Msa0181200:five_prime_UTR 30.0%
!! TTACAAGTGTTGGAGAGATA+GGG + chr1_4:69364844-69364863 None:intergenic 35.0%
!! ATTACAAGTGTTGGAGAGAT+AGG + chr1_4:69364845-69364864 None:intergenic 35.0%
! TTTTCTCAGTTTCCATTTGC+TGG - chr1_4:69365743-69365762 Msa0181200:intron 35.0%
GATTGTAGTTGGATGGTAAT+TGG + chr1_4:69365826-69365845 None:intergenic 35.0%
GGGTAGGTTTAAAGAAATCA+AGG + chr1_4:69365908-69365927 None:intergenic 35.0%
! ATGTTTGGATGCAAGGTATT+GGG + chr1_4:69365928-69365947 None:intergenic 35.0%
TTAATGCATGTTTGGATGCA+AGG + chr1_4:69365935-69365954 None:intergenic 35.0%
TCACTTATAAACTCGTGTTC+AGG + chr1_4:69366058-69366077 None:intergenic 35.0%
!! ACTGATAAAACCGGTTTGTA+AGG + chr1_4:69366093-69366112 None:intergenic 35.0%
CGAACTCAAACTGATAAAAC+CGG + chr1_4:69366102-69366121 None:intergenic 35.0%
!! CGGTTTTATCAGTTTGAGTT+CGG - chr1_4:69366100-69366119 Msa0181200:intron 35.0%
AACTTTCATCCATAACAGCA+TGG - chr1_4:69366634-69366653 Msa0181200:intron 35.0%
ACTTTCATCCATAACAGCAT+GGG - chr1_4:69366635-69366654 Msa0181200:intron 35.0%
AGCTGAATTGAGAAAACTTG+AGG - chr1_4:69366683-69366702 Msa0181200:intron 35.0%
GGAACAACTAGAGAAGAAAA+AGG - chr1_4:69366792-69366811 Msa0181200:CDS 35.0%
AGAAAAGAAGGCTATGATTG+AGG - chr1_4:69366870-69366889 Msa0181200:CDS 35.0%
AGAATTACTCAAAGCAGAAG+AGG - chr1_4:69366906-69366925 Msa0181200:CDS 35.0%
ACTCACTCATTCTTAGATGT+TGG - chr1_4:69367208-69367227 Msa0181200:five_prime_UTR 35.0%
ACTAAAAGTCTTAGCTGGGT+GGG + chr1_4:69364745-69364764 None:intergenic 40.0%
TACTAAAAGTCTTAGCTGGG+TGG + chr1_4:69364746-69364765 None:intergenic 40.0%
TCCAAAGAAAGATGTAGCTG+AGG - chr1_4:69365107-69365126 Msa0181200:CDS 40.0%
TCCTCAGCTACATCTTTCTT+TGG + chr1_4:69365111-69365130 None:intergenic 40.0%
GGAGGAGGATTATTATCTTG+TGG + chr1_4:69365147-69365166 None:intergenic 40.0%
TGTCCTATTGAGTTTCCTGA+AGG - chr1_4:69365602-69365621 Msa0181200:intron 40.0%
GTCCTATTGAGTTTCCTGAA+GGG - chr1_4:69365603-69365622 Msa0181200:intron 40.0%
TCCTATTGAGTTTCCTGAAG+GGG - chr1_4:69365604-69365623 Msa0181200:intron 40.0%
! TTTTCTTTGTTACCCCCTTC+AGG + chr1_4:69365620-69365639 None:intergenic 40.0%
CATGTTTGGATGCAAGGTAT+TGG + chr1_4:69365929-69365948 None:intergenic 40.0%
!! TGGTCTTGTAATCAGTGCAT+TGG - chr1_4:69366032-69366051 Msa0181200:intron 40.0%
!! TAAAACCGGTTTGTAAGGTG+AGG + chr1_4:69366088-69366107 None:intergenic 40.0%
ATTATATGCGGTCGATGTGA+AGG + chr1_4:69366193-69366212 None:intergenic 40.0%
TGCTGAAGAGAAACCAAAAG+AGG - chr1_4:69366267-69366286 Msa0181200:intron 40.0%
GCTGAAGAGAAACCAAAAGA+GGG - chr1_4:69366268-69366287 Msa0181200:intron 40.0%
CAAGAGTTGCAACAGAAAAG+AGG - chr1_4:69366408-69366427 Msa0181200:intron 40.0%
GCTGTCACTAATCAAAGCAT+GGG - chr1_4:69366430-69366449 Msa0181200:intron 40.0%
CATTAAGCATTAGATGGTGC+AGG + chr1_4:69366494-69366513 None:intergenic 40.0%
CAAAATAGCAGCACTTCACA+AGG - chr1_4:69366840-69366859 Msa0181200:CDS 40.0%
GCTATGATTGAGGCAAAGAA+AGG - chr1_4:69366880-69366899 Msa0181200:CDS 40.0%
AAGAGGTAGCTGCAAAATAC+AGG - chr1_4:69366923-69366942 Msa0181200:CDS 40.0%
AGAGGTAGCTGCAAAATACA+GGG - chr1_4:69366924-69366943 Msa0181200:CDS 40.0%
ACAGCTCCAAAGAAACTCTT+TGG - chr1_4:69366955-69366974 Msa0181200:five_prime_UTR 40.0%
GGCTACAATGTTCAAGATTG+TGG - chr1_4:69366988-69367007 Msa0181200:five_prime_UTR 40.0%
GAGTCAGTGGTCTAATACAA+AGG + chr1_4:69367192-69367211 None:intergenic 40.0%
TCTAAGAATGAGTGAGTCAG+TGG + chr1_4:69367205-69367224 None:intergenic 40.0%
TGGTAGGTACTCTTGTGAGT+TGG + chr1_4:69364717-69364736 None:intergenic 45.0%
! TCTTAGCTGGGTGGGTTTAT+TGG + chr1_4:69364737-69364756 None:intergenic 45.0%
!! AAGTGTTGGAGAGATAGGGT+TGG + chr1_4:69364840-69364859 None:intergenic 45.0%
!! CATCTTTCTTTGGAGCCTCA+GGG + chr1_4:69365101-69365120 None:intergenic 45.0%
!! ACATCTTTCTTTGGAGCCTC+AGG + chr1_4:69365102-69365121 None:intergenic 45.0%
TTGGAGTCATCAACAACAGG+AGG + chr1_4:69365174-69365193 None:intergenic 45.0%
!! GCTTTGGAGTCATCAACAAC+AGG + chr1_4:69365177-69365196 None:intergenic 45.0%
CTGAGCTATGACAAGAGCTT+TGG + chr1_4:69365193-69365212 None:intergenic 45.0%
CCTATTGAGTTTCCTGAAGG+GGG - chr1_4:69365605-69365624 Msa0181200:intron 45.0%
!! TTGGATGCAAGGTATTGGGT+AGG + chr1_4:69365924-69365943 None:intergenic 45.0%
CGAATCCTCACCTTACAAAC+CGG - chr1_4:69366080-69366099 Msa0181200:intron 45.0%
GAAGAGAAACCAAAAGAGGG+CGG - chr1_4:69366271-69366290 Msa0181200:intron 45.0%
GGCTGTCACTAATCAAAGCA+TGG - chr1_4:69366429-69366448 Msa0181200:intron 45.0%
ACTGTTCTCCCATGCTGTTA+TGG + chr1_4:69366646-69366665 None:intergenic 45.0%
CAAGGCAGCTGAAGAAAAGA+AGG - chr1_4:69366858-69366877 Msa0181200:CDS 45.0%
! AGCTGGGTGGGTTTATTGGT+AGG + chr1_4:69364733-69364752 None:intergenic 50.0%
GTAGTCGTCTCAGTTGAAGC+AGG + chr1_4:69365057-69365076 None:intergenic 50.0%
TCATCAACAACAGGAGGAGG+AGG + chr1_4:69365168-69365187 None:intergenic 50.0%
GAGTCATCAACAACAGGAGG+AGG + chr1_4:69365171-69365190 None:intergenic 50.0%
CCCCCTTCAGGAAACTCAAT+AGG + chr1_4:69365608-69365627 None:intergenic 50.0%
GCTGCAAAATACAGGGCAAC+TGG - chr1_4:69366931-69366950 Msa0181200:exon 50.0%
GTTGGCACAGCGCACCAATA+GGG + chr1_4:69364699-69364718 None:intergenic 55.0%
AGTTGGCACAGCGCACCAAT+AGG + chr1_4:69364700-69364719 None:intergenic 55.0%
! TCAGTTGAAGCAGGTGGTGG+TGG + chr1_4:69365048-69365067 None:intergenic 55.0%
GTCTCAGTTGAAGCAGGTGG+TGG + chr1_4:69365051-69365070 None:intergenic 55.0%
GTCGTCTCAGTTGAAGCAGG+TGG + chr1_4:69365054-69365073 None:intergenic 55.0%
!! TTCTTTGGAGCCTCAGGGAG+AGG + chr1_4:69365096-69365115 None:intergenic 55.0%
TCAACAACAGGAGGAGGAGG+AGG + chr1_4:69365165-69365184 None:intergenic 55.0%
! TCGATAGAGCCGCCCTCTTT+TGG + chr1_4:69366283-69366302 None:intergenic 55.0%
!! GTTGAAGCAGGTGGTGGTGG+AGG + chr1_4:69365045-69365064 None:intergenic 60.0%
CTCAGGGAGAGGAGGAGAAG+GGG + chr1_4:69365085-69365104 None:intergenic 60.0%
CCTCAGGGAGAGGAGGAGAA+GGG + chr1_4:69365086-69365105 None:intergenic 60.0%
! TTTGGAGCCTCAGGGAGAGG+AGG + chr1_4:69365093-69365112 None:intergenic 60.0%
ACAACAGGAGGAGGAGGAGG+AGG + chr1_4:69365162-69365181 None:intergenic 60.0%
CCCTTCTCCTCCTCTCCCTG+AGG - chr1_4:69365083-69365102 Msa0181200:CDS 65.0%
GCCTCAGGGAGAGGAGGAGA+AGG + chr1_4:69365087-69365106 None:intergenic 65.0%
GAGGGCGGCTCTATCGACCG+TGG - chr1_4:69366286-69366305 Msa0181200:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 69364690 69367246 69364690 ID=Msa0181200;Name=Msa0181200
chr1_4 mRNA 69364690 69367246 69364690 ID=Msa0181200-mRNA-1;Parent=Msa0181200;Name=Msa0181200-mRNA-1;_AED=0.09;_eAED=0.09;_QI=301|1|1|1|1|1|5|259|213
chr1_4 exon 69364690 69365143 69364690 ID=Msa0181200-mRNA-1:exon:23016;Parent=Msa0181200-mRNA-1
chr1_4 exon 69365232 69365313 69365232 ID=Msa0181200-mRNA-1:exon:23015;Parent=Msa0181200-mRNA-1
chr1_4 exon 69365453 69365540 69365453 ID=Msa0181200-mRNA-1:exon:23014;Parent=Msa0181200-mRNA-1
chr1_4 exon 69365629 69365682 69365629 ID=Msa0181200-mRNA-1:exon:23013;Parent=Msa0181200-mRNA-1
chr1_4 exon 69366723 69367246 69366723 ID=Msa0181200-mRNA-1:exon:23012;Parent=Msa0181200-mRNA-1
chr1_4 five_prime_UTR 69366946 69367246 69366946 ID=Msa0181200-mRNA-1:five_prime_utr;Parent=Msa0181200-mRNA-1
chr1_4 CDS 69366723 69366945 69366723 ID=Msa0181200-mRNA-1:cds;Parent=Msa0181200-mRNA-1
chr1_4 CDS 69365629 69365682 69365629 ID=Msa0181200-mRNA-1:cds;Parent=Msa0181200-mRNA-1
chr1_4 CDS 69365453 69365540 69365453 ID=Msa0181200-mRNA-1:cds;Parent=Msa0181200-mRNA-1
chr1_4 CDS 69365232 69365313 69365232 ID=Msa0181200-mRNA-1:cds;Parent=Msa0181200-mRNA-1
chr1_4 CDS 69364949 69365143 69364949 ID=Msa0181200-mRNA-1:cds;Parent=Msa0181200-mRNA-1
chr1_4 three_prime_UTR 69364690 69364948 69364690 ID=Msa0181200-mRNA-1:three_prime_utr;Parent=Msa0181200-mRNA-1
Gene Sequence

>Msa0181200

ATGACAGATCAAGAGCAACCAAAAAAGATAGAATCTGAATCAACATCGAATCCTCCACCACCACCTGCTTCAACTGAGACGACTACTACTACCCCTTCTCCTCCTCTCCCTGAGGCTCCAAAGAAAGATGTAGCTGAGGAGAAAGCTGTAATTCCACAAGATAATAATCCTCCTCCTCCTCCTCCTGTTGTTGATGACTCCAAAGCTCTTGTCATAGCTCAGAAGACTGATGAAGTTGCTGAAGAGAAACCAAAAGAGGGCGGCTCTATCGACCGTGATGCTGTGCTTACAAGAGTTGCAACAGAAAAGAGGCTGTCACTAATCAAAGCATGGGAAGAAAGTGAAAAATCAAAAGCAGAGAACAAAGCTCAGCGAAAACTTTCATCCATAACAGCATGGGAGAACAGTAAGAAAGCTGCTAAAGAAGCTGAATTGAGAAAACTTGAGGAACAACTAGAGAAGAAAAAGGGAGAATATGCTGAGAAAATGAAAAACAAAATAGCAGCACTTCACAAGGCAGCTGAAGAAAAGAAGGCTATGATTGAGGCAAAGAAAGGTGAAGAATTACTCAAAGCAGAAGAGGTAGCTGCAAAATACAGGGCAACTGGAACAGCTCCAAAGAAACTCTTTGGTCTTTTCTAA
Protein sequence

>Msa0181200

MTDQEQPKKIESESTSNPPPPPASTETTTTTPSPPLPEAPKKDVAEEKAVIPQDNNPPPPPPVVDDSKALVIAQKTDEVAEEKPKEGGSIDRDAVLTRVATEKRLSLIKAWEESEKSKAENKAQRKLSSITAWENSKKAAKEAELRKLEEQLEKKKGEYAEKMKNKIAALHKAAEEKKAMIEAKKGEELLKAEEVAAKYRATGTAPKKLFGLF*