AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0182800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182800 MtrunA17_Chr1g0210031 92.857 126 4 3 56 180 2 123 8.92e-66 197
Msa0182800 MtrunA17_Chr5g0422991 65.041 123 38 3 56 178 2 119 9.84e-44 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0182800 AT1G68585.1 49.451 91 38 3 82 166 24 112 2.85e-18 76.6

Find 35 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGACTAGTCTTGCATCTT+TGG 0.189720 1_4:+71621339 Msa0182800:CDS
AAGATTGTTATTATGATAAA+AGG 0.195948 1_4:+71621266 Msa0182800:CDS
CGTTTGATAATGGCAACTTT+AGG 0.228347 1_4:-71622260 None:intergenic
GGGGAAGGAGTCTTGTTTAA+TGG 0.240314 1_4:-71622239 None:intergenic
AGAAGGTGATACTTACATTT+AGG 0.262994 1_4:+71622171 Msa0182800:CDS
GTTTGATAATGGCAACTTTA+GGG 0.304764 1_4:-71622259 None:intergenic
TAATGTCAGCAACTCTCTTC+TGG 0.333777 1_4:-71621524 None:intergenic
GATAGAACAATTTCATCTCT+TGG 0.366031 1_4:-71621169 None:intergenic
ATAGAACAATTTCATCTCTT+GGG 0.377484 1_4:-71621168 None:intergenic
GCGGAATATCCGTTTGATAA+TGG 0.404318 1_4:-71622270 None:intergenic
GGAGTCTTTGTCACTGCCAC+TGG 0.407535 1_4:+71621360 Msa0182800:CDS
ATGCTTATGACTTGAGTTGT+TGG 0.430989 1_4:-71621145 None:intergenic
ACTTGAGTTGTTGGTGGGGT+TGG 0.461160 1_4:-71621136 None:intergenic
AATAAACACTCAGTTTCTGT+TGG 0.469610 1_4:+71621204 Msa0182800:CDS
CCCAGAAAGAGGAAAACTCT+TGG 0.478273 1_4:-71621320 None:intergenic
CTTATGACTTGAGTTGTTGG+TGG 0.494044 1_4:-71621142 None:intergenic
GTCAGCAACTCTCTTCTGGC+AGG 0.495054 1_4:-71621520 None:intergenic
GTCAATTGTGGTGAATGTAT+TGG 0.498672 1_4:+71622145 Msa0182800:CDS
TAAGAAGTTAAATAAAACTA+AGG 0.508098 1_4:+71621237 Msa0182800:CDS
TCACAAATAGTAGAGATGTA+AGG 0.514214 1_4:+71622448 Msa0182800:three_prime_UTR
TTATGACTTGAGTTGTTGGT+GGG 0.515275 1_4:-71621141 None:intergenic
TTGTTATTGCTTTCTCAACA+TGG 0.518334 1_4:+71622421 Msa0182800:three_prime_UTR
AGGAATCTTCAGAAATCAGC+TGG 0.529337 1_4:+71621286 Msa0182800:CDS
CTAAAGTTGCCATTATCAAA+CGG 0.531333 1_4:+71622261 Msa0182800:CDS
TATAATTACTAATTAACCAC+TGG 0.531693 1_4:-71622297 None:intergenic
GCAAGACTAGTCCCAGAAAG+AGG 0.539833 1_4:-71621331 None:intergenic
ATAATGGCAACTTTAGGGGA+AGG 0.548777 1_4:-71622254 None:intergenic
GGTGAATGTATTGGAAAAGA+AGG 0.579398 1_4:+71622154 Msa0182800:CDS
CGGATATTCCGCTCATCCAG+TGG 0.579718 1_4:+71622281 Msa0182800:CDS
ACACTCAGTTTCTGTTGGAG+AGG 0.587433 1_4:+71621209 Msa0182800:CDS
AACAGAATCTTATGCACATG+TGG 0.606171 1_4:-71622337 None:intergenic
TATGACTTGAGTTGTTGGTG+GGG 0.608328 1_4:-71621140 None:intergenic
CTAATTAACCACTGGATGAG+CGG 0.609688 1_4:-71622289 None:intergenic
TTTGATAATGGCAACTTTAG+GGG 0.615844 1_4:-71622258 None:intergenic
AGCAGAGACAGAGTCAATTG+TGG 0.625282 1_4:+71622133 Msa0182800:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAGTTAAATAAAACTA+AGG + chr1_4:71621237-71621256 Msa0182800:CDS 15.0%
!!! AAGATTGTTATTATGATAAA+AGG + chr1_4:71621266-71621285 Msa0182800:CDS 15.0%
!! TCTATATATATAGTGTTTAA+TGG + chr1_4:71621436-71621455 Msa0182800:intron 15.0%
!! TAACAGTAAATAACAATAAT+TGG - chr1_4:71621893-71621912 None:intergenic 15.0%
!! TAAGAAGTTAAATAAAACTA+AGG + chr1_4:71621237-71621256 Msa0182800:CDS 15.0%
!!! AAGATTGTTATTATGATAAA+AGG + chr1_4:71621266-71621285 Msa0182800:CDS 15.0%
!! TCTATATATATAGTGTTTAA+TGG + chr1_4:71621436-71621455 Msa0182800:intron 15.0%
!! TAACAGTAAATAACAATAAT+TGG - chr1_4:71621893-71621912 None:intergenic 15.0%
!! AAAACTCAAAATACAGTAAA+AGG - chr1_4:71621400-71621419 None:intergenic 20.0%
!! GACATTATTACAAAAATGAA+TGG + chr1_4:71621540-71621559 Msa0182800:CDS 20.0%
!!! ATTTTTGTTCTGTTGAAATA+AGG + chr1_4:71621581-71621600 Msa0182800:intron 20.0%
!! AGTAAATAACAATAATTGGA+AGG - chr1_4:71621889-71621908 None:intergenic 20.0%
!! TATAATTACTAATTAACCAC+TGG - chr1_4:71622300-71622319 None:intergenic 20.0%
!! AAAACTCAAAATACAGTAAA+AGG - chr1_4:71621400-71621419 None:intergenic 20.0%
!! GACATTATTACAAAAATGAA+TGG + chr1_4:71621540-71621559 Msa0182800:CDS 20.0%
!!! ATTTTTGTTCTGTTGAAATA+AGG + chr1_4:71621581-71621600 Msa0182800:intron 20.0%
!! AGTAAATAACAATAATTGGA+AGG - chr1_4:71621889-71621908 None:intergenic 20.0%
!! TATAATTACTAATTAACCAC+TGG - chr1_4:71622300-71622319 None:intergenic 20.0%
!!! AAAAGCCATTTTCACTTTTT+TGG + chr1_4:71621096-71621115 Msa0182800:CDS 25.0%
! AATAGCCAAAAAAGTGAAAA+TGG - chr1_4:71621104-71621123 None:intergenic 25.0%
! ATAGAACAATTTCATCTCTT+GGG - chr1_4:71621171-71621190 None:intergenic 25.0%
! AAATAAGGAGATATCATGTA+TGG + chr1_4:71621596-71621615 Msa0182800:intron 25.0%
! TGCTGAAAAATTATAGATAC+GGG - chr1_4:71622117-71622136 None:intergenic 25.0%
! CTGCTGAAAAATTATAGATA+CGG - chr1_4:71622118-71622137 None:intergenic 25.0%
!!! AAAAGCCATTTTCACTTTTT+TGG + chr1_4:71621096-71621115 Msa0182800:CDS 25.0%
! AATAGCCAAAAAAGTGAAAA+TGG - chr1_4:71621104-71621123 None:intergenic 25.0%
! ATAGAACAATTTCATCTCTT+GGG - chr1_4:71621171-71621190 None:intergenic 25.0%
! AAATAAGGAGATATCATGTA+TGG + chr1_4:71621596-71621615 Msa0182800:intron 25.0%
! TGCTGAAAAATTATAGATAC+GGG - chr1_4:71622117-71622136 None:intergenic 25.0%
! CTGCTGAAAAATTATAGATA+CGG - chr1_4:71622118-71622137 None:intergenic 25.0%
GATAGAACAATTTCATCTCT+TGG - chr1_4:71621172-71621191 None:intergenic 30.0%
AATAAACACTCAGTTTCTGT+TGG + chr1_4:71621204-71621223 Msa0182800:CDS 30.0%
AGGGCTTATAAATTATACAG+TGG - chr1_4:71621783-71621802 None:intergenic 30.0%
! TCTTTCACTGATTATATGCA+TGG + chr1_4:71621946-71621965 Msa0182800:intron 30.0%
CTGAAAAATTATAGATACGG+GGG - chr1_4:71622115-71622134 None:intergenic 30.0%
GCTGAAAAATTATAGATACG+GGG - chr1_4:71622116-71622135 None:intergenic 30.0%
AGAAGGTGATACTTACATTT+AGG + chr1_4:71622171-71622190 Msa0182800:CDS 30.0%
AGGATATCAAAAACAACTGT+TGG + chr1_4:71622191-71622210 Msa0182800:CDS 30.0%
!! TTTGATAATGGCAACTTTAG+GGG - chr1_4:71622261-71622280 None:intergenic 30.0%
!! GTTTGATAATGGCAACTTTA+GGG - chr1_4:71622262-71622281 None:intergenic 30.0%
CTAAAGTTGCCATTATCAAA+CGG + chr1_4:71622261-71622280 Msa0182800:CDS 30.0%
! TTGTTATTGCTTTCTCAACA+TGG + chr1_4:71622421-71622440 Msa0182800:three_prime_UTR 30.0%
TCACAAATAGTAGAGATGTA+AGG + chr1_4:71622448-71622467 Msa0182800:three_prime_UTR 30.0%
GATAGAACAATTTCATCTCT+TGG - chr1_4:71621172-71621191 None:intergenic 30.0%
AATAAACACTCAGTTTCTGT+TGG + chr1_4:71621204-71621223 Msa0182800:CDS 30.0%
AGGGCTTATAAATTATACAG+TGG - chr1_4:71621783-71621802 None:intergenic 30.0%
! TCTTTCACTGATTATATGCA+TGG + chr1_4:71621946-71621965 Msa0182800:intron 30.0%
CTGAAAAATTATAGATACGG+GGG - chr1_4:71622115-71622134 None:intergenic 30.0%
GCTGAAAAATTATAGATACG+GGG - chr1_4:71622116-71622135 None:intergenic 30.0%
AGAAGGTGATACTTACATTT+AGG + chr1_4:71622171-71622190 Msa0182800:CDS 30.0%
AGGATATCAAAAACAACTGT+TGG + chr1_4:71622191-71622210 Msa0182800:CDS 30.0%
!! TTTGATAATGGCAACTTTAG+GGG - chr1_4:71622261-71622280 None:intergenic 30.0%
!! GTTTGATAATGGCAACTTTA+GGG - chr1_4:71622262-71622281 None:intergenic 30.0%
CTAAAGTTGCCATTATCAAA+CGG + chr1_4:71622261-71622280 Msa0182800:CDS 30.0%
! TTGTTATTGCTTTCTCAACA+TGG + chr1_4:71622421-71622440 Msa0182800:three_prime_UTR 30.0%
TCACAAATAGTAGAGATGTA+AGG + chr1_4:71622448-71622467 Msa0182800:three_prime_UTR 30.0%
! TTATGACTTGAGTTGTTGGT+GGG - chr1_4:71621144-71621163 None:intergenic 35.0%
! ATGCTTATGACTTGAGTTGT+TGG - chr1_4:71621148-71621167 None:intergenic 35.0%
!! GTGATTGTTTTGTTGAGAAC+AGG + chr1_4:71621458-71621477 Msa0182800:intron 35.0%
TCTTCTTGCAAAGCAAATAG+AGG - chr1_4:71621709-71621728 None:intergenic 35.0%
TCTATTTGCTTTGCAAGAAG+AGG + chr1_4:71621708-71621727 Msa0182800:intron 35.0%
CAAGCTAGAGGAATGTTATA+GGG + chr1_4:71621820-71621839 Msa0182800:intron 35.0%
AGTCATAGATATCAGCACTA+TGG - chr1_4:71621846-71621865 None:intergenic 35.0%
GTCAATTGTGGTGAATGTAT+TGG + chr1_4:71622145-71622164 Msa0182800:CDS 35.0%
GGTGAATGTATTGGAAAAGA+AGG + chr1_4:71622154-71622173 Msa0182800:CDS 35.0%
!! CGTTTGATAATGGCAACTTT+AGG - chr1_4:71622263-71622282 None:intergenic 35.0%
AACAGAATCTTATGCACATG+TGG - chr1_4:71622340-71622359 None:intergenic 35.0%
! TTATGACTTGAGTTGTTGGT+GGG - chr1_4:71621144-71621163 None:intergenic 35.0%
! ATGCTTATGACTTGAGTTGT+TGG - chr1_4:71621148-71621167 None:intergenic 35.0%
!! GTGATTGTTTTGTTGAGAAC+AGG + chr1_4:71621458-71621477 Msa0182800:intron 35.0%
TCTTCTTGCAAAGCAAATAG+AGG - chr1_4:71621709-71621728 None:intergenic 35.0%
TCTATTTGCTTTGCAAGAAG+AGG + chr1_4:71621708-71621727 Msa0182800:intron 35.0%
CAAGCTAGAGGAATGTTATA+GGG + chr1_4:71621820-71621839 Msa0182800:intron 35.0%
AGTCATAGATATCAGCACTA+TGG - chr1_4:71621846-71621865 None:intergenic 35.0%
GTCAATTGTGGTGAATGTAT+TGG + chr1_4:71622145-71622164 Msa0182800:CDS 35.0%
GGTGAATGTATTGGAAAAGA+AGG + chr1_4:71622154-71622173 Msa0182800:CDS 35.0%
!! CGTTTGATAATGGCAACTTT+AGG - chr1_4:71622263-71622282 None:intergenic 35.0%
AACAGAATCTTATGCACATG+TGG - chr1_4:71622340-71622359 None:intergenic 35.0%
! TATGACTTGAGTTGTTGGTG+GGG - chr1_4:71621143-71621162 None:intergenic 40.0%
! CTTATGACTTGAGTTGTTGG+TGG - chr1_4:71621145-71621164 None:intergenic 40.0%
AGGAATCTTCAGAAATCAGC+TGG + chr1_4:71621286-71621305 Msa0182800:CDS 40.0%
! TCCAAGAGTTTTCCTCTTTC+TGG + chr1_4:71621319-71621338 Msa0182800:CDS 40.0%
! CCAAGAGTTTTCCTCTTTCT+GGG + chr1_4:71621320-71621339 Msa0182800:CDS 40.0%
GGTTCAAAACTACATACCAG+TGG - chr1_4:71621379-71621398 None:intergenic 40.0%
TAATGTCAGCAACTCTCTTC+TGG - chr1_4:71621527-71621546 None:intergenic 40.0%
AAGGAGATATCATGTATGGC+TGG + chr1_4:71621600-71621619 Msa0182800:intron 40.0%
AGAGGTTAAAGAAGACACAG+AGG + chr1_4:71621726-71621745 Msa0182800:intron 40.0%
CAGTGGCAACATAAACAGTA+AGG - chr1_4:71621766-71621785 None:intergenic 40.0%
TGCAGACAAAAACACATGCA+GGG - chr1_4:71621802-71621821 None:intergenic 40.0%
TTGCAGACAAAAACACATGC+AGG - chr1_4:71621803-71621822 None:intergenic 40.0%
!! TGTTTTTGTCTGCAAGCTAG+AGG + chr1_4:71621808-71621827 Msa0182800:intron 40.0%
GCAAGCTAGAGGAATGTTAT+AGG + chr1_4:71621819-71621838 Msa0182800:intron 40.0%
! ATAATGGCAACTTTAGGGGA+AGG - chr1_4:71622257-71622276 None:intergenic 40.0%
GCGGAATATCCGTTTGATAA+TGG - chr1_4:71622273-71622292 None:intergenic 40.0%
CTAATTAACCACTGGATGAG+CGG - chr1_4:71622292-71622311 None:intergenic 40.0%
! TATGACTTGAGTTGTTGGTG+GGG - chr1_4:71621143-71621162 None:intergenic 40.0%
! CTTATGACTTGAGTTGTTGG+TGG - chr1_4:71621145-71621164 None:intergenic 40.0%
AGGAATCTTCAGAAATCAGC+TGG + chr1_4:71621286-71621305 Msa0182800:CDS 40.0%
! TCCAAGAGTTTTCCTCTTTC+TGG + chr1_4:71621319-71621338 Msa0182800:CDS 40.0%
! CCAAGAGTTTTCCTCTTTCT+GGG + chr1_4:71621320-71621339 Msa0182800:CDS 40.0%
GGTTCAAAACTACATACCAG+TGG - chr1_4:71621379-71621398 None:intergenic 40.0%
TAATGTCAGCAACTCTCTTC+TGG - chr1_4:71621527-71621546 None:intergenic 40.0%
AAGGAGATATCATGTATGGC+TGG + chr1_4:71621600-71621619 Msa0182800:intron 40.0%
AGAGGTTAAAGAAGACACAG+AGG + chr1_4:71621726-71621745 Msa0182800:intron 40.0%
CAGTGGCAACATAAACAGTA+AGG - chr1_4:71621766-71621785 None:intergenic 40.0%
TGCAGACAAAAACACATGCA+GGG - chr1_4:71621802-71621821 None:intergenic 40.0%
TTGCAGACAAAAACACATGC+AGG - chr1_4:71621803-71621822 None:intergenic 40.0%
!! TGTTTTTGTCTGCAAGCTAG+AGG + chr1_4:71621808-71621827 Msa0182800:intron 40.0%
GCAAGCTAGAGGAATGTTAT+AGG + chr1_4:71621819-71621838 Msa0182800:intron 40.0%
! ATAATGGCAACTTTAGGGGA+AGG - chr1_4:71622257-71622276 None:intergenic 40.0%
GCGGAATATCCGTTTGATAA+TGG - chr1_4:71622273-71622292 None:intergenic 40.0%
CTAATTAACCACTGGATGAG+CGG - chr1_4:71622292-71622311 None:intergenic 40.0%
ACACTCAGTTTCTGTTGGAG+AGG + chr1_4:71621209-71621228 Msa0182800:CDS 45.0%
CCCAGAAAGAGGAAAACTCT+TGG - chr1_4:71621323-71621342 None:intergenic 45.0%
!! TGGGACTAGTCTTGCATCTT+TGG + chr1_4:71621339-71621358 Msa0182800:CDS 45.0%
!! GTTTTGTTGAGAACAGGTGC+AGG + chr1_4:71621464-71621483 Msa0182800:intron 45.0%
AGCAGAGACAGAGTCAATTG+TGG + chr1_4:71622133-71622152 Msa0182800:intron 45.0%
!! GGGGAAGGAGTCTTGTTTAA+TGG - chr1_4:71622242-71622261 None:intergenic 45.0%
ACACTCAGTTTCTGTTGGAG+AGG + chr1_4:71621209-71621228 Msa0182800:CDS 45.0%
CCCAGAAAGAGGAAAACTCT+TGG - chr1_4:71621323-71621342 None:intergenic 45.0%
!! TGGGACTAGTCTTGCATCTT+TGG + chr1_4:71621339-71621358 Msa0182800:CDS 45.0%
!! GTTTTGTTGAGAACAGGTGC+AGG + chr1_4:71621464-71621483 Msa0182800:intron 45.0%
AGCAGAGACAGAGTCAATTG+TGG + chr1_4:71622133-71622152 Msa0182800:intron 45.0%
!! GGGGAAGGAGTCTTGTTTAA+TGG - chr1_4:71622242-71622261 None:intergenic 45.0%
! ACTTGAGTTGTTGGTGGGGT+TGG - chr1_4:71621139-71621158 None:intergenic 50.0%
! GCAAGACTAGTCCCAGAAAG+AGG - chr1_4:71621334-71621353 None:intergenic 50.0%
GACACAGAGGAAAAGAGCTG+TGG + chr1_4:71621739-71621758 Msa0182800:intron 50.0%
! ACTTGAGTTGTTGGTGGGGT+TGG - chr1_4:71621139-71621158 None:intergenic 50.0%
! GCAAGACTAGTCCCAGAAAG+AGG - chr1_4:71621334-71621353 None:intergenic 50.0%
GACACAGAGGAAAAGAGCTG+TGG + chr1_4:71621739-71621758 Msa0182800:intron 50.0%
GGAGTCTTTGTCACTGCCAC+TGG + chr1_4:71621360-71621379 Msa0182800:CDS 55.0%
GTCAGCAACTCTCTTCTGGC+AGG - chr1_4:71621523-71621542 None:intergenic 55.0%
CGGATATTCCGCTCATCCAG+TGG + chr1_4:71622281-71622300 Msa0182800:CDS 55.0%
GGAGTCTTTGTCACTGCCAC+TGG + chr1_4:71621360-71621379 Msa0182800:CDS 55.0%
GTCAGCAACTCTCTTCTGGC+AGG - chr1_4:71621523-71621542 None:intergenic 55.0%
CGGATATTCCGCTCATCCAG+TGG + chr1_4:71622281-71622300 Msa0182800:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 71621094 71622486 71621094 ID=Msa0182800;Name=Msa0182800
chr1_4 mRNA 71621094 71622486 71621094 ID=Msa0182800-mRNA-1;Parent=Msa0182800;Name=Msa0182800-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|1|1|1|1|3|179|180
chr1_4 exon 71621094 71621381 71621094 ID=Msa0182800-mRNA-1:exon:23973;Parent=Msa0182800-mRNA-1
chr1_4 exon 71621480 71621561 71621480 ID=Msa0182800-mRNA-1:exon:23974;Parent=Msa0182800-mRNA-1
chr1_4 exon 71622135 71622486 71622135 ID=Msa0182800-mRNA-1:exon:23975;Parent=Msa0182800-mRNA-1
chr1_4 CDS 71621094 71621381 71621094 ID=Msa0182800-mRNA-1:cds;Parent=Msa0182800-mRNA-1
chr1_4 CDS 71621480 71621561 71621480 ID=Msa0182800-mRNA-1:cds;Parent=Msa0182800-mRNA-1
chr1_4 CDS 71622135 71622307 71622135 ID=Msa0182800-mRNA-1:cds;Parent=Msa0182800-mRNA-1
chr1_4 three_prime_UTR 71622308 71622486 71622308 ID=Msa0182800-mRNA-1:three_prime_utr;Parent=Msa0182800-mRNA-1
Gene Sequence

>Msa0182800

AAAAAAGCCATTTTCACTTTTTTGGCTATTTTTCATTTTCTGCCAACCCCACCAACAACTCAAGTCATAAGCATCCCAAGAGATGAAATTGTTCTATCAACTACAAGCTTAATAAACACTCAGTTTCTGTTGGAGAGGAGTAATAAGAAGTTAAATAAAACTAAGGCTTTTGAAGATTGTTATTATGATAAAAGGAATCTTCAGAAATCAGCTGGTATAAAAATGTCCAAGAGTTTTCCTCTTTCTGGGACTAGTCTTGCATCTTTGGAGTCTTTGTCACTGCCACTGGTGCAGGAAGTTGTTCTTTCAGCAGATATGCAATGTGAAACCTGCCAGAAGAGAGTTGCTGACATTATTACAAAAATGAATGCAGAGACAGAGTCAATTGTGGTGAATGTATTGGAAAAGAAGGTGATACTTACATTTAGGATATCAAAAACAACTGTTGGTAAATTAATCTCAAAGAAAGTTACGCCATTAAACAAGACTCCTTCCCCTAAAGTTGCCATTATCAAACGGATATTCCGCTCATCCAGTGGTTAA

Protein sequence

>Msa0182800

KKAIFTFLAIFHFLPTPPTTQVISIPRDEIVLSTTSLINTQFLLERSNKKLNKTKAFEDCYYDKRNLQKSAGIKMSKSFPLSGTSLASLESLSLPLVQEVVLSADMQCETCQKRVADIITKMNAETESIVVNVLEKKVILTFRISKTTVGKLISKKVTPLNKTPSPKVAIIKRIFRSSSG*