AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0046470


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0046470 MtrunA17_Chr1g0210031 92.857 126 4 3 56 180 2 123 8.92e-66 197
Msa0046470 MtrunA17_Chr5g0422991 65.041 123 38 3 56 178 2 119 9.84e-44 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0046470 AT1G68585.1 49.451 91 38 3 82 166 24 112 2.85e-18 76.6

Find 35 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGACTAGTCTTGCATCTT+TGG 0.189720 1_1:-78625257 Msa0046470:CDS
AAGATTGTTATTATGATAAA+AGG 0.195948 1_1:-78625330 Msa0046470:CDS
CGTTTGATAATGGCAACTTT+AGG 0.228347 1_1:+78624336 None:intergenic
GGGGAAGGAGTCTTGTTTAA+TGG 0.240314 1_1:+78624357 None:intergenic
AGAAGGTGATACTTACATTT+AGG 0.262994 1_1:-78624425 Msa0046470:CDS
GTTTGATAATGGCAACTTTA+GGG 0.304764 1_1:+78624337 None:intergenic
TAATGTCAGCAACTCTCTTC+TGG 0.333777 1_1:+78625072 None:intergenic
GATAGAACAATTTCATCTCT+TGG 0.366031 1_1:+78625427 None:intergenic
ATAGAACAATTTCATCTCTT+GGG 0.377484 1_1:+78625428 None:intergenic
GCGGAATATCCGTTTGATAA+TGG 0.404318 1_1:+78624326 None:intergenic
GGAGTCTTTGTCACTGCCAC+TGG 0.407535 1_1:-78625236 Msa0046470:intron
ATGCTTATGACTTGAGTTGT+TGG 0.430989 1_1:+78625451 None:intergenic
ACTTGAGTTGTTGGTGGGGT+TGG 0.461160 1_1:+78625460 None:intergenic
AATAAACACTCAGTTTCTGT+TGG 0.469610 1_1:-78625392 Msa0046470:CDS
CCCAGAAAGAGGAAAACTCT+TGG 0.478273 1_1:+78625276 None:intergenic
CTTATGACTTGAGTTGTTGG+TGG 0.494044 1_1:+78625454 None:intergenic
GTCAGCAACTCTCTTCTGGC+AGG 0.495054 1_1:+78625076 None:intergenic
GTCAATTGTGGTGAATGTAT+TGG 0.498672 1_1:-78624451 Msa0046470:CDS
TAAGAAGTTAAATAAAACTA+AGG 0.508098 1_1:-78625359 Msa0046470:CDS
TCACAAATAGTAGAGATGTA+AGG 0.514214 1_1:-78624148 Msa0046470:three_prime_UTR
TTATGACTTGAGTTGTTGGT+GGG 0.515275 1_1:+78625455 None:intergenic
TTGTTATTGCTTTCTCAACA+TGG 0.518334 1_1:-78624175 Msa0046470:three_prime_UTR
AGGAATCTTCAGAAATCAGC+TGG 0.529337 1_1:-78625310 Msa0046470:CDS
CTAAAGTTGCCATTATCAAA+CGG 0.531333 1_1:-78624335 Msa0046470:CDS
TATAATTACTAATTAACCAC+TGG 0.531693 1_1:+78624299 None:intergenic
GCAAGACTAGTCCCAGAAAG+AGG 0.539833 1_1:+78625265 None:intergenic
ATAATGGCAACTTTAGGGGA+AGG 0.548777 1_1:+78624342 None:intergenic
GGTGAATGTATTGGAAAAGA+AGG 0.579398 1_1:-78624442 Msa0046470:CDS
CGGATATTCCGCTCATCCAG+TGG 0.579718 1_1:-78624315 Msa0046470:CDS
ACACTCAGTTTCTGTTGGAG+AGG 0.587433 1_1:-78625387 Msa0046470:CDS
AACAGAATCTTATGCACATG+TGG 0.606171 1_1:+78624259 None:intergenic
TATGACTTGAGTTGTTGGTG+GGG 0.608328 1_1:+78625456 None:intergenic
CTAATTAACCACTGGATGAG+CGG 0.609688 1_1:+78624307 None:intergenic
TTTGATAATGGCAACTTTAG+GGG 0.615844 1_1:+78624338 None:intergenic
AGCAGAGACAGAGTCAATTG+TGG 0.625282 1_1:-78624463 Msa0046470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAGTTAAATAAAACTA+AGG - chr1_1:78624275-78624294 Msa0046470:three_prime_UTR 15.0%
!!! AAGATTGTTATTATGATAAA+AGG - chr1_1:78624304-78624323 Msa0046470:exon 15.0%
!! TCTATATATATAGTGTTTAA+TGG - chr1_1:78624474-78624493 Msa0046470:intron 15.0%
!! TAACAGTAAATAACAATAAT+TGG + chr1_1:78624931-78624950 None:intergenic 15.0%
!! TAAGAAGTTAAATAAAACTA+AGG - chr1_1:78624275-78624294 Msa0046470:three_prime_UTR 15.0%
!!! AAGATTGTTATTATGATAAA+AGG - chr1_1:78624304-78624323 Msa0046470:exon 15.0%
!! TCTATATATATAGTGTTTAA+TGG - chr1_1:78624474-78624493 Msa0046470:intron 15.0%
!! TAACAGTAAATAACAATAAT+TGG + chr1_1:78624931-78624950 None:intergenic 15.0%
!! AAAACTCAAAATACAGTAAA+AGG + chr1_1:78624438-78624457 None:intergenic 20.0%
!! GACATTATTACAAAAATGAA+TGG - chr1_1:78624578-78624597 Msa0046470:intron 20.0%
!!! ATTTTTGTTCTGTTGAAATA+AGG - chr1_1:78624619-78624638 Msa0046470:intron 20.0%
!! AGTAAATAACAATAATTGGA+AGG + chr1_1:78624927-78624946 None:intergenic 20.0%
!! TATAATTACTAATTAACCAC+TGG + chr1_1:78625338-78625357 None:intergenic 20.0%
!! AAAACTCAAAATACAGTAAA+AGG + chr1_1:78624438-78624457 None:intergenic 20.0%
!! GACATTATTACAAAAATGAA+TGG - chr1_1:78624578-78624597 Msa0046470:intron 20.0%
!!! ATTTTTGTTCTGTTGAAATA+AGG - chr1_1:78624619-78624638 Msa0046470:intron 20.0%
!! AGTAAATAACAATAATTGGA+AGG + chr1_1:78624927-78624946 None:intergenic 20.0%
!! TATAATTACTAATTAACCAC+TGG + chr1_1:78625338-78625357 None:intergenic 20.0%
!!! AAAAGCCATTTTCACTTTTT+TGG - chr1_1:78624134-78624153 Msa0046470:three_prime_UTR 25.0%
! AATAGCCAAAAAAGTGAAAA+TGG + chr1_1:78624142-78624161 None:intergenic 25.0%
! ATAGAACAATTTCATCTCTT+GGG + chr1_1:78624209-78624228 None:intergenic 25.0%
! AAATAAGGAGATATCATGTA+TGG - chr1_1:78624634-78624653 Msa0046470:intron 25.0%
! TGCTGAAAAATTATAGATAC+GGG + chr1_1:78625155-78625174 None:intergenic 25.0%
! CTGCTGAAAAATTATAGATA+CGG + chr1_1:78625156-78625175 None:intergenic 25.0%
!!! AAAAGCCATTTTCACTTTTT+TGG - chr1_1:78624134-78624153 Msa0046470:three_prime_UTR 25.0%
! AATAGCCAAAAAAGTGAAAA+TGG + chr1_1:78624142-78624161 None:intergenic 25.0%
! ATAGAACAATTTCATCTCTT+GGG + chr1_1:78624209-78624228 None:intergenic 25.0%
! AAATAAGGAGATATCATGTA+TGG - chr1_1:78624634-78624653 Msa0046470:intron 25.0%
! TGCTGAAAAATTATAGATAC+GGG + chr1_1:78625155-78625174 None:intergenic 25.0%
! CTGCTGAAAAATTATAGATA+CGG + chr1_1:78625156-78625175 None:intergenic 25.0%
GATAGAACAATTTCATCTCT+TGG + chr1_1:78624210-78624229 None:intergenic 30.0%
AATAAACACTCAGTTTCTGT+TGG - chr1_1:78624242-78624261 Msa0046470:three_prime_UTR 30.0%
AGGGCTTATAAATTATACAG+TGG + chr1_1:78624821-78624840 None:intergenic 30.0%
! TCTTTCACTGATTATATGCA+TGG - chr1_1:78624984-78625003 Msa0046470:intron 30.0%
CTGAAAAATTATAGATACGG+GGG + chr1_1:78625153-78625172 None:intergenic 30.0%
GCTGAAAAATTATAGATACG+GGG + chr1_1:78625154-78625173 None:intergenic 30.0%
AGAAGGTGATACTTACATTT+AGG - chr1_1:78625209-78625228 Msa0046470:intron 30.0%
AGGATATCAAAAACAACTGT+TGG - chr1_1:78625229-78625248 Msa0046470:intron 30.0%
!! TTTGATAATGGCAACTTTAG+GGG + chr1_1:78625299-78625318 None:intergenic 30.0%
!! GTTTGATAATGGCAACTTTA+GGG + chr1_1:78625300-78625319 None:intergenic 30.0%
CTAAAGTTGCCATTATCAAA+CGG - chr1_1:78625299-78625318 Msa0046470:CDS 30.0%
! TTGTTATTGCTTTCTCAACA+TGG - chr1_1:78625459-78625478 Msa0046470:CDS 30.0%
TCACAAATAGTAGAGATGTA+AGG - chr1_1:78625486-78625505 Msa0046470:CDS 30.0%
GATAGAACAATTTCATCTCT+TGG + chr1_1:78624210-78624229 None:intergenic 30.0%
AATAAACACTCAGTTTCTGT+TGG - chr1_1:78624242-78624261 Msa0046470:three_prime_UTR 30.0%
AGGGCTTATAAATTATACAG+TGG + chr1_1:78624821-78624840 None:intergenic 30.0%
! TCTTTCACTGATTATATGCA+TGG - chr1_1:78624984-78625003 Msa0046470:intron 30.0%
CTGAAAAATTATAGATACGG+GGG + chr1_1:78625153-78625172 None:intergenic 30.0%
GCTGAAAAATTATAGATACG+GGG + chr1_1:78625154-78625173 None:intergenic 30.0%
AGAAGGTGATACTTACATTT+AGG - chr1_1:78625209-78625228 Msa0046470:intron 30.0%
AGGATATCAAAAACAACTGT+TGG - chr1_1:78625229-78625248 Msa0046470:intron 30.0%
!! TTTGATAATGGCAACTTTAG+GGG + chr1_1:78625299-78625318 None:intergenic 30.0%
!! GTTTGATAATGGCAACTTTA+GGG + chr1_1:78625300-78625319 None:intergenic 30.0%
CTAAAGTTGCCATTATCAAA+CGG - chr1_1:78625299-78625318 Msa0046470:CDS 30.0%
! TTGTTATTGCTTTCTCAACA+TGG - chr1_1:78625459-78625478 Msa0046470:CDS 30.0%
TCACAAATAGTAGAGATGTA+AGG - chr1_1:78625486-78625505 Msa0046470:CDS 30.0%
! TTATGACTTGAGTTGTTGGT+GGG + chr1_1:78624182-78624201 None:intergenic 35.0%
! ATGCTTATGACTTGAGTTGT+TGG + chr1_1:78624186-78624205 None:intergenic 35.0%
!! GTGATTGTTTTGTTGAGAAC+AGG - chr1_1:78624496-78624515 Msa0046470:intron 35.0%
TCTTCTTGCAAAGCAAATAG+AGG + chr1_1:78624747-78624766 None:intergenic 35.0%
TCTATTTGCTTTGCAAGAAG+AGG - chr1_1:78624746-78624765 Msa0046470:intron 35.0%
CAAGCTAGAGGAATGTTATA+GGG - chr1_1:78624858-78624877 Msa0046470:intron 35.0%
AGTCATAGATATCAGCACTA+TGG + chr1_1:78624884-78624903 None:intergenic 35.0%
GTCAATTGTGGTGAATGTAT+TGG - chr1_1:78625183-78625202 Msa0046470:intron 35.0%
GGTGAATGTATTGGAAAAGA+AGG - chr1_1:78625192-78625211 Msa0046470:intron 35.0%
!! CGTTTGATAATGGCAACTTT+AGG + chr1_1:78625301-78625320 None:intergenic 35.0%
AACAGAATCTTATGCACATG+TGG + chr1_1:78625378-78625397 None:intergenic 35.0%
! TTATGACTTGAGTTGTTGGT+GGG + chr1_1:78624182-78624201 None:intergenic 35.0%
! ATGCTTATGACTTGAGTTGT+TGG + chr1_1:78624186-78624205 None:intergenic 35.0%
!! GTGATTGTTTTGTTGAGAAC+AGG - chr1_1:78624496-78624515 Msa0046470:intron 35.0%
TCTTCTTGCAAAGCAAATAG+AGG + chr1_1:78624747-78624766 None:intergenic 35.0%
TCTATTTGCTTTGCAAGAAG+AGG - chr1_1:78624746-78624765 Msa0046470:intron 35.0%
CAAGCTAGAGGAATGTTATA+GGG - chr1_1:78624858-78624877 Msa0046470:intron 35.0%
AGTCATAGATATCAGCACTA+TGG + chr1_1:78624884-78624903 None:intergenic 35.0%
GTCAATTGTGGTGAATGTAT+TGG - chr1_1:78625183-78625202 Msa0046470:intron 35.0%
GGTGAATGTATTGGAAAAGA+AGG - chr1_1:78625192-78625211 Msa0046470:intron 35.0%
!! CGTTTGATAATGGCAACTTT+AGG + chr1_1:78625301-78625320 None:intergenic 35.0%
AACAGAATCTTATGCACATG+TGG + chr1_1:78625378-78625397 None:intergenic 35.0%
! TATGACTTGAGTTGTTGGTG+GGG + chr1_1:78624181-78624200 None:intergenic 40.0%
! CTTATGACTTGAGTTGTTGG+TGG + chr1_1:78624183-78624202 None:intergenic 40.0%
AGGAATCTTCAGAAATCAGC+TGG - chr1_1:78624324-78624343 Msa0046470:CDS 40.0%
! TCCAAGAGTTTTCCTCTTTC+TGG - chr1_1:78624357-78624376 Msa0046470:CDS 40.0%
! CCAAGAGTTTTCCTCTTTCT+GGG - chr1_1:78624358-78624377 Msa0046470:CDS 40.0%
GGTTCAAAACTACATACCAG+TGG + chr1_1:78624417-78624436 None:intergenic 40.0%
TAATGTCAGCAACTCTCTTC+TGG + chr1_1:78624565-78624584 None:intergenic 40.0%
AAGGAGATATCATGTATGGC+TGG - chr1_1:78624638-78624657 Msa0046470:intron 40.0%
AGAGGTTAAAGAAGACACAG+AGG - chr1_1:78624764-78624783 Msa0046470:intron 40.0%
CAGTGGCAACATAAACAGTA+AGG + chr1_1:78624804-78624823 None:intergenic 40.0%
TGCAGACAAAAACACATGCA+GGG + chr1_1:78624840-78624859 None:intergenic 40.0%
TTGCAGACAAAAACACATGC+AGG + chr1_1:78624841-78624860 None:intergenic 40.0%
!! TGTTTTTGTCTGCAAGCTAG+AGG - chr1_1:78624846-78624865 Msa0046470:intron 40.0%
GCAAGCTAGAGGAATGTTAT+AGG - chr1_1:78624857-78624876 Msa0046470:intron 40.0%
! ATAATGGCAACTTTAGGGGA+AGG + chr1_1:78625295-78625314 None:intergenic 40.0%
GCGGAATATCCGTTTGATAA+TGG + chr1_1:78625311-78625330 None:intergenic 40.0%
CTAATTAACCACTGGATGAG+CGG + chr1_1:78625330-78625349 None:intergenic 40.0%
! TATGACTTGAGTTGTTGGTG+GGG + chr1_1:78624181-78624200 None:intergenic 40.0%
! CTTATGACTTGAGTTGTTGG+TGG + chr1_1:78624183-78624202 None:intergenic 40.0%
AGGAATCTTCAGAAATCAGC+TGG - chr1_1:78624324-78624343 Msa0046470:CDS 40.0%
! TCCAAGAGTTTTCCTCTTTC+TGG - chr1_1:78624357-78624376 Msa0046470:CDS 40.0%
! CCAAGAGTTTTCCTCTTTCT+GGG - chr1_1:78624358-78624377 Msa0046470:CDS 40.0%
GGTTCAAAACTACATACCAG+TGG + chr1_1:78624417-78624436 None:intergenic 40.0%
TAATGTCAGCAACTCTCTTC+TGG + chr1_1:78624565-78624584 None:intergenic 40.0%
AAGGAGATATCATGTATGGC+TGG - chr1_1:78624638-78624657 Msa0046470:intron 40.0%
AGAGGTTAAAGAAGACACAG+AGG - chr1_1:78624764-78624783 Msa0046470:intron 40.0%
CAGTGGCAACATAAACAGTA+AGG + chr1_1:78624804-78624823 None:intergenic 40.0%
TGCAGACAAAAACACATGCA+GGG + chr1_1:78624840-78624859 None:intergenic 40.0%
TTGCAGACAAAAACACATGC+AGG + chr1_1:78624841-78624860 None:intergenic 40.0%
!! TGTTTTTGTCTGCAAGCTAG+AGG - chr1_1:78624846-78624865 Msa0046470:intron 40.0%
GCAAGCTAGAGGAATGTTAT+AGG - chr1_1:78624857-78624876 Msa0046470:intron 40.0%
! ATAATGGCAACTTTAGGGGA+AGG + chr1_1:78625295-78625314 None:intergenic 40.0%
GCGGAATATCCGTTTGATAA+TGG + chr1_1:78625311-78625330 None:intergenic 40.0%
CTAATTAACCACTGGATGAG+CGG + chr1_1:78625330-78625349 None:intergenic 40.0%
ACACTCAGTTTCTGTTGGAG+AGG - chr1_1:78624247-78624266 Msa0046470:three_prime_UTR 45.0%
CCCAGAAAGAGGAAAACTCT+TGG + chr1_1:78624361-78624380 None:intergenic 45.0%
!! TGGGACTAGTCTTGCATCTT+TGG - chr1_1:78624377-78624396 Msa0046470:CDS 45.0%
!! GTTTTGTTGAGAACAGGTGC+AGG - chr1_1:78624502-78624521 Msa0046470:intron 45.0%
AGCAGAGACAGAGTCAATTG+TGG - chr1_1:78625171-78625190 Msa0046470:intron 45.0%
!! GGGGAAGGAGTCTTGTTTAA+TGG + chr1_1:78625280-78625299 None:intergenic 45.0%
ACACTCAGTTTCTGTTGGAG+AGG - chr1_1:78624247-78624266 Msa0046470:three_prime_UTR 45.0%
CCCAGAAAGAGGAAAACTCT+TGG + chr1_1:78624361-78624380 None:intergenic 45.0%
!! TGGGACTAGTCTTGCATCTT+TGG - chr1_1:78624377-78624396 Msa0046470:CDS 45.0%
!! GTTTTGTTGAGAACAGGTGC+AGG - chr1_1:78624502-78624521 Msa0046470:intron 45.0%
AGCAGAGACAGAGTCAATTG+TGG - chr1_1:78625171-78625190 Msa0046470:intron 45.0%
!! GGGGAAGGAGTCTTGTTTAA+TGG + chr1_1:78625280-78625299 None:intergenic 45.0%
! ACTTGAGTTGTTGGTGGGGT+TGG + chr1_1:78624177-78624196 None:intergenic 50.0%
! GCAAGACTAGTCCCAGAAAG+AGG + chr1_1:78624372-78624391 None:intergenic 50.0%
GACACAGAGGAAAAGAGCTG+TGG - chr1_1:78624777-78624796 Msa0046470:intron 50.0%
! ACTTGAGTTGTTGGTGGGGT+TGG + chr1_1:78624177-78624196 None:intergenic 50.0%
! GCAAGACTAGTCCCAGAAAG+AGG + chr1_1:78624372-78624391 None:intergenic 50.0%
GACACAGAGGAAAAGAGCTG+TGG - chr1_1:78624777-78624796 Msa0046470:intron 50.0%
GGAGTCTTTGTCACTGCCAC+TGG - chr1_1:78624398-78624417 Msa0046470:CDS 55.0%
GTCAGCAACTCTCTTCTGGC+AGG + chr1_1:78624561-78624580 None:intergenic 55.0%
CGGATATTCCGCTCATCCAG+TGG - chr1_1:78625319-78625338 Msa0046470:CDS 55.0%
GGAGTCTTTGTCACTGCCAC+TGG - chr1_1:78624398-78624417 Msa0046470:CDS 55.0%
GTCAGCAACTCTCTTCTGGC+AGG + chr1_1:78624561-78624580 None:intergenic 55.0%
CGGATATTCCGCTCATCCAG+TGG - chr1_1:78625319-78625338 Msa0046470:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 78624132 78625524 78624132 ID=Msa0046470;Name=Msa0046470
chr1_1 mRNA 78624132 78625524 78624132 ID=Msa0046470-mRNA-1;Parent=Msa0046470;Name=Msa0046470-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|1|1|1|1|3|179|180
chr1_1 exon 78624132 78624483 78624132 ID=Msa0046470-mRNA-1:exon:27072;Parent=Msa0046470-mRNA-1
chr1_1 exon 78625057 78625138 78625057 ID=Msa0046470-mRNA-1:exon:27071;Parent=Msa0046470-mRNA-1
chr1_1 exon 78625237 78625524 78625237 ID=Msa0046470-mRNA-1:exon:27070;Parent=Msa0046470-mRNA-1
chr1_1 CDS 78625237 78625524 78625237 ID=Msa0046470-mRNA-1:cds;Parent=Msa0046470-mRNA-1
chr1_1 CDS 78625057 78625138 78625057 ID=Msa0046470-mRNA-1:cds;Parent=Msa0046470-mRNA-1
chr1_1 CDS 78624311 78624483 78624311 ID=Msa0046470-mRNA-1:cds;Parent=Msa0046470-mRNA-1
chr1_1 three_prime_UTR 78624132 78624310 78624132 ID=Msa0046470-mRNA-1:three_prime_utr;Parent=Msa0046470-mRNA-1
Gene Sequence

>Msa0046470

AAAAAAGCCATTTTCACTTTTTTGGCTATTTTTCATTTTCTGCCAACCCCACCAACAACTCAAGTCATAAGCATCCCAAGAGATGAAATTGTTCTATCAACTACAAGCTTAATAAACACTCAGTTTCTGTTGGAGAGGAGTAATAAGAAGTTAAATAAAACTAAGGCTTTTGAAGATTGTTATTATGATAAAAGGAATCTTCAGAAATCAGCTGGTATAAAAATGTCCAAGAGTTTTCCTCTTTCTGGGACTAGTCTTGCATCTTTGGAGTCTTTGTCACTGCCACTGGTGCAGGAAGTTGTTCTTTCAGCAGATATGCAATGTGAAACCTGCCAGAAGAGAGTTGCTGACATTATTACAAAAATGAATGCAGAGACAGAGTCAATTGTGGTGAATGTATTGGAAAAGAAGGTGATACTTACATTTAGGATATCAAAAACAACTGTTGGTAAATTAATCTCAAAGAAAGTTACGCCATTAAACAAGACTCCTTCCCCTAAAGTTGCCATTATCAAACGGATATTCCGCTCATCCAGTGGTTAA

Protein sequence

>Msa0046470

KKAIFTFLAIFHFLPTPPTTQVISIPRDEIVLSTTSLINTQFLLERSNKKLNKTKAFEDCYYDKRNLQKSAGIKMSKSFPLSGTSLASLESLSLPLVQEVVLSADMQCETCQKRVADIITKMNAETESIVVNVLEKKVILTFRISKTTVGKLISKKVTPLNKTPSPKVAIIKRIFRSSSG*