AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184490 MtrunA17_Chr1g0206091 100.000 96 0 0 1 96 1 96 1.73e-67 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184490 AT1G61780.1 88.776 98 9 1 1 96 1 98 7.77e-60 178

Find 45 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCTTGGATATTCAAAT+TGG 0.227939 1_4:-74113881 Msa0184490:five_prime_UTR
CAATTTGAATATCCAAGATT+TGG 0.257745 1_4:+74113882 None:intergenic
TGAAGGGAACCTGTTCTTCT+TGG 0.293706 1_4:+74113577 None:intergenic
CCCCTCCCTCTCATAAGTTT+TGG 0.330996 1_4:+74110127 None:intergenic
AATTAGCTGAAAAGGGGTAT+AGG 0.350950 1_4:-74113913 Msa0184490:five_prime_UTR
GAAGGCTGTCGACGTCGTTT+TGG 0.355325 1_4:-74113972 Msa0184490:five_prime_UTR
GCACCTTCTTTCCATTTGTC+TGG 0.356085 1_4:+74113653 None:intergenic
ATTAGCTGAAAAGGGGTATA+GGG 0.371633 1_4:-74113912 Msa0184490:five_prime_UTR
TTAGACTATATTATATATAA+AGG 0.375911 1_4:+74110339 None:intergenic
TATGAGAGGGAGGGGCGTTT+TGG 0.376336 1_4:-74110119 Msa0184490:three_prime_UTR
AACTCCTTTCCAAGAAGAAC+AGG 0.405714 1_4:-74113586 Msa0184490:intron
ACGACACGAATTAGCTGAAA+AGG 0.416736 1_4:-74113921 Msa0184490:five_prime_UTR
AGAGGAACCTTACGTCTTGC+CGG 0.429446 1_4:+74110087 None:intergenic
CCTTGTAGATGGACCCCTTA+CGG 0.465158 1_4:-74110642 Msa0184490:intron
GTTGCTTTGTTTATAAAACT+TGG 0.466176 1_4:+74110416 None:intergenic
AGGTGATAGTACCAGACAAA+TGG 0.467879 1_4:-74113664 Msa0184490:CDS
CACACGTGTGCTTACTCAAA+AGG 0.468563 1_4:-74110564 Msa0184490:intron
TGCACTTGGTTGTTCCGTAA+GGG 0.477894 1_4:+74110628 None:intergenic
ATGCACTTGGTTGTTCCGTA+AGG 0.478636 1_4:+74110627 None:intergenic
CTGCTTACAAATGATGCACT+TGG 0.484104 1_4:+74110614 None:intergenic
CGACACGAATTAGCTGAAAA+GGG 0.490307 1_4:-74113920 Msa0184490:five_prime_UTR
CACTTTGATCAATAGCATAG+AGG 0.500824 1_4:+74110069 None:intergenic
ATAAACAAAGCAACGTGTAG+TGG 0.523993 1_4:-74110407 Msa0184490:exon
TTGTAAGCAGCAAGTGCACC+AGG 0.524349 1_4:-74110601 Msa0184490:CDS
GCAGACCATCGCAGTCGCTA+AGG 0.528087 1_4:+74113804 None:intergenic
CCAAAACTTATGAGAGGGAG+GGG 0.533985 1_4:-74110127 Msa0184490:three_prime_UTR
GCACTTGGTTGTTCCGTAAG+GGG 0.542962 1_4:+74110629 None:intergenic
TGTGACAGTACTTGCCATCC+TGG 0.544753 1_4:+74110583 None:intergenic
GGAACCTGTTCTTCTTGGAA+AGG 0.545948 1_4:+74113582 None:intergenic
GATGAATCCGGCAAGACGTA+AGG 0.551859 1_4:-74110094 Msa0184490:three_prime_UTR
GTACCAGACAAATGGAAAGA+AGG 0.553949 1_4:-74113656 Msa0184490:CDS
ACCAAAACTTATGAGAGGGA+GGG 0.578094 1_4:-74110128 Msa0184490:three_prime_UTR
TTTGACCAAAACTTATGAGA+GGG 0.582502 1_4:-74110132 Msa0184490:three_prime_UTR
GACCAAAACTTATGAGAGGG+AGG 0.596320 1_4:-74110129 Msa0184490:three_prime_UTR
TAGATCTCATAAATACTCGA+AGG 0.603857 1_4:-74113990 Msa0184490:five_prime_UTR
ATTTGACCAAAACTTATGAG+AGG 0.607189 1_4:-74110133 Msa0184490:three_prime_UTR
GGTGCAAGCAATACTACTGA+AGG 0.608421 1_4:-74113635 Msa0184490:CDS
AGCAATACTACTGAAGGCGG+TGG 0.615667 1_4:-74113629 Msa0184490:CDS
AATTTGAGTTGATCTTAGCA+AGG 0.619178 1_4:+74110153 None:intergenic
AGAATCCTTAGCGACTGCGA+TGG 0.623608 1_4:-74113809 Msa0184490:exon
AAGCAGCAAGTGCACCAGGA+TGG 0.635149 1_4:-74110597 Msa0184490:CDS
GCAGGAGTCTGTGCGATGTG+TGG 0.652505 1_4:-74110456 Msa0184490:intron
GACACGAATTAGCTGAAAAG+GGG 0.659143 1_4:-74113919 Msa0184490:five_prime_UTR
GCAAGCAATACTACTGAAGG+CGG 0.661649 1_4:-74113632 Msa0184490:CDS
AGGTCAGAAGAAATTATCGA+AGG 0.666813 1_4:-74113684 Msa0184490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTATTTTTTTAATT+AGG - chr1_4:74110516-74110535 Msa0184490:intron 0.0%
!!! ATTATTTATTTTTTTAATTA+GGG - chr1_4:74110517-74110536 Msa0184490:intron 0.0%
!!! AATAATTTTAGTTTTGATAT+TGG - chr1_4:74110612-74110631 Msa0184490:CDS 10.0%
!!! AAAAAGTAAATAAATTTTAG+GGG + chr1_4:74111659-74111678 None:intergenic 10.0%
!!! GAAAAAGTAAATAAATTTTA+GGG + chr1_4:74111660-74111679 None:intergenic 10.0%
!!! TGAAAAAGTAAATAAATTTT+AGG + chr1_4:74111661-74111680 None:intergenic 10.0%
!!! TAAATTTTGAGTAATATTTA+AGG - chr1_4:74112613-74112632 Msa0184490:intron 10.0%
!!! AATATATTTTTTGTATAGTA+TGG - chr1_4:74112642-74112661 Msa0184490:intron 10.0%
!! TTAGACTATATTATATATAA+AGG + chr1_4:74113721-74113740 None:intergenic 10.0%
!!! GTAAATTCAATTGAATTTTT+TGG - chr1_4:74110330-74110349 Msa0184490:three_prime_UTR 15.0%
!!! AAAAGTAAATAAATTTTAGG+GGG + chr1_4:74111658-74111677 None:intergenic 15.0%
!! AAAAAGATAAGTAAAAAGAA+CGG + chr1_4:74112351-74112370 None:intergenic 15.0%
!! ATTTACAAAAGCTAGAAATA+TGG + chr1_4:74110316-74110335 None:intergenic 20.0%
!! AATAAATAATTGAAGGGTTT+AGG + chr1_4:74110507-74110526 None:intergenic 20.0%
!!! TATTTGGTCTGTTTTTTTTT+TGG - chr1_4:74111258-74111277 Msa0184490:intron 20.0%
!!! TTTTATATGTAGACTTGATA+TGG - chr1_4:74111327-74111346 Msa0184490:intron 20.0%
!!! GTTTGAAAACAGTTATTTTT+TGG - chr1_4:74111519-74111538 Msa0184490:intron 20.0%
!!! AAAGTAAATAAATTTTAGGG+GGG + chr1_4:74111657-74111676 None:intergenic 20.0%
!!! CCTGTTAAAAAATAAAGTTT+TGG + chr1_4:74111685-74111704 None:intergenic 20.0%
!!! CCAAAACTTTATTTTTTAAC+AGG - chr1_4:74111682-74111701 Msa0184490:intron 20.0%
!!! TTATTTTTTAACAGGACATA+AGG - chr1_4:74111690-74111709 Msa0184490:intron 20.0%
!!! TTTTTTTTGGTTGATTTTGT+GGG - chr1_4:74111971-74111990 Msa0184490:intron 20.0%
!! AAAGGAAAACATTTAGTATT+GGG + chr1_4:74112193-74112212 None:intergenic 20.0%
!! AAAAGGAAAACATTTAGTAT+TGG + chr1_4:74112194-74112213 None:intergenic 20.0%
!!! TATTTTTCTTTTTGAAAGAC+AGG + chr1_4:74112250-74112269 None:intergenic 20.0%
!! AATAATCTAAGTAGAAAGAA+AGG + chr1_4:74112302-74112321 None:intergenic 20.0%
!! AAGCTATTGAACATTAAAAT+TGG - chr1_4:74112849-74112868 Msa0184490:intron 20.0%
!!! ATATTTTTGATATATCTGAG+TGG - chr1_4:74112912-74112931 Msa0184490:intron 20.0%
!! TGAACATTTACAAAAAGTAA+TGG + chr1_4:74113220-74113239 None:intergenic 20.0%
!! CTGTAAAAAGTTAATATTGT+GGG - chr1_4:74113771-74113790 Msa0184490:intron 20.0%
! CAATTTGAATATCCAAGATT+TGG + chr1_4:74110178-74110197 None:intergenic 25.0%
! CAAATCTTGGATATTCAAAT+TGG - chr1_4:74110176-74110195 Msa0184490:three_prime_UTR 25.0%
! TAATTTGAATTCGATGTTGT+AGG - chr1_4:74110353-74110372 Msa0184490:three_prime_UTR 25.0%
!! GTATGAAAAGCATAGTAATT+TGG - chr1_4:74110539-74110558 Msa0184490:intron 25.0%
!! TGGTATAACTTCAAATTTTG+AGG - chr1_4:74110667-74110686 Msa0184490:intron 25.0%
!!! TATAACTTCAAATTTTGAGG+TGG - chr1_4:74110670-74110689 Msa0184490:intron 25.0%
!!! ATAACTTCAAATTTTGAGGT+GGG - chr1_4:74110671-74110690 Msa0184490:intron 25.0%
!!! ATAAGTTTTATGTGGTTGTT+CGG - chr1_4:74110749-74110768 Msa0184490:intron 25.0%
! TAGCTTATGATATTGTTCAT+AGG - chr1_4:74110787-74110806 Msa0184490:intron 25.0%
! TTAAGATAGCTTATGAAAAC+AGG - chr1_4:74110838-74110857 Msa0184490:intron 25.0%
! AAAAAATGGAACAAAAAGAG+TGG + chr1_4:74111035-74111054 None:intergenic 25.0%
!! AGCATCGAGTTTAAAAAAAA+TGG + chr1_4:74111049-74111068 None:intergenic 25.0%
!! GAAAGAAACCTTATGTTTTT+AGG - chr1_4:74111372-74111391 Msa0184490:intron 25.0%
! AATAAAATGCAAGGGAAAAA+CGG + chr1_4:74111568-74111587 None:intergenic 25.0%
! TGGCATTAAATAAAATGCAA+GGG + chr1_4:74111576-74111595 None:intergenic 25.0%
! ATGGCATTAAATAAAATGCA+AGG + chr1_4:74111577-74111596 None:intergenic 25.0%
!!! GTTTTTTTTGGTTGATTTTG+TGG - chr1_4:74111970-74111989 Msa0184490:intron 25.0%
!!! AGAGTAAGGTGTTTTATAAA+AGG + chr1_4:74112211-74112230 None:intergenic 25.0%
!!! AATAGCTTTCTTTTTTGGAA+TGG + chr1_4:74112837-74112856 None:intergenic 25.0%
!!! TGTTCAATAGCTTTCTTTTT+TGG + chr1_4:74112842-74112861 None:intergenic 25.0%
! AGACATATTATGATGAGATA+TGG + chr1_4:74112983-74113002 None:intergenic 25.0%
!!! TGTAATTCTTGCTTTTTGAA+TGG - chr1_4:74113092-74113111 Msa0184490:intron 25.0%
! ACAGATCAAAGTATAGTAAT+TGG + chr1_4:74113300-74113319 None:intergenic 25.0%
! CAAATTACAAAAGGAGAAAA+GGG + chr1_4:74113336-74113355 None:intergenic 25.0%
! TCAAATTACAAAAGGAGAAA+AGG + chr1_4:74113337-74113356 None:intergenic 25.0%
! GTTCACATAACATAAAAACA+GGG + chr1_4:74113366-74113385 None:intergenic 25.0%
! TGTTCACATAACATAAAAAC+AGG + chr1_4:74113367-74113386 None:intergenic 25.0%
! TTGAATTTATTCCTTGTAGA+TGG - chr1_4:74113404-74113423 Msa0184490:intron 25.0%
!!! GTTGCTTTGTTTATAAAACT+TGG + chr1_4:74113644-74113663 None:intergenic 25.0%
! GCTGTAAAAAGTTAATATTG+TGG - chr1_4:74113770-74113789 Msa0184490:intron 25.0%
!!! ACAATAGTGTTTTTTCGATA+AGG + chr1_4:74113832-74113851 None:intergenic 25.0%
! TATCGAAAAAACACTATTGT+TGG - chr1_4:74113832-74113851 Msa0184490:intron 25.0%
TAGATCTCATAAATACTCGA+AGG - chr1_4:74110067-74110086 Msa0184490:three_prime_UTR 30.0%
ACAAAAGCTAGAAATATGGA+TGG + chr1_4:74110312-74110331 None:intergenic 30.0%
!! AGTTTTGATATTGGAAGCTT+TGG - chr1_4:74110621-74110640 Msa0184490:CDS 30.0%
!! GTTTTGATATTGGAAGCTTT+GGG - chr1_4:74110622-74110641 Msa0184490:CDS 30.0%
! AATTTGAAGTTATACCAAGC+AGG + chr1_4:74110664-74110683 None:intergenic 30.0%
!!! TAACTTCAAATTTTGAGGTG+GGG - chr1_4:74110672-74110691 Msa0184490:intron 30.0%
!! TAAGTTTTATGTGGTTGTTC+GGG - chr1_4:74110750-74110769 Msa0184490:intron 30.0%
! ACAACTTGTTTCCAGTTTAT+AGG + chr1_4:74110959-74110978 None:intergenic 30.0%
CCTTAAACATCAAATCCTAT+TGG + chr1_4:74111226-74111245 None:intergenic 30.0%
CCAATAGGATTTGATGTTTA+AGG - chr1_4:74111223-74111242 Msa0184490:intron 30.0%
! ACTTGATATGGTTAGTTGTA+TGG - chr1_4:74111339-74111358 Msa0184490:intron 30.0%
!!! AGAAACCTTATGTTTTTAGG+TGG - chr1_4:74111375-74111394 Msa0184490:intron 30.0%
AACTACCACCTAAAAACATA+AGG + chr1_4:74111383-74111402 None:intergenic 30.0%
TGAACAAGTGAATCAATTGT+TGG - chr1_4:74111442-74111461 Msa0184490:intron 30.0%
AATCAATTGTTGGATGTCAT+TGG - chr1_4:74111452-74111471 Msa0184490:intron 30.0%
ATCAATTGTTGGATGTCATT+GGG - chr1_4:74111453-74111472 Msa0184490:intron 30.0%
AGTGAGCATGATGAAATTAT+GGG + chr1_4:74111618-74111637 None:intergenic 30.0%
AAGTGAGCATGATGAAATTA+TGG + chr1_4:74111619-74111638 None:intergenic 30.0%
! TCTCGTTTTATATGCGTTTT+TGG + chr1_4:74112017-74112036 None:intergenic 30.0%
ATCAAACTAGGTTAAAGTTG+TGG - chr1_4:74112164-74112183 Msa0184490:intron 30.0%
GGTGTATTAGTAAAAGAGTA+AGG + chr1_4:74112225-74112244 None:intergenic 30.0%
!! TTTCTTTTTGAAAGACAGGT+GGG + chr1_4:74112246-74112265 None:intergenic 30.0%
!! TTTTCTTTTTGAAAGACAGG+TGG + chr1_4:74112247-74112266 None:intergenic 30.0%
TATATGCTAGTGTTATGTTG+TGG + chr1_4:74112796-74112815 None:intergenic 30.0%
TGGAATGGAAAATGTTAGTA+TGG + chr1_4:74112822-74112841 None:intergenic 30.0%
ACAATGTAACCAAAACAGTT+CGG + chr1_4:74112947-74112966 None:intergenic 30.0%
GCGCTTATATTTAGTAATCA+CGG - chr1_4:74113062-74113081 Msa0184490:intron 30.0%
! GTTTAGAATGAGTGATGTTA+TGG - chr1_4:74113147-74113166 Msa0184490:intron 30.0%
TTGGATAAAAAACTTGCCAA+TGG + chr1_4:74113281-74113300 None:intergenic 30.0%
!! ACTTTGATCTGTTGTTTCAA+TGG - chr1_4:74113308-74113327 Msa0184490:intron 30.0%
!! CTTTGATCTGTTGTTTCAAT+GGG - chr1_4:74113309-74113328 Msa0184490:intron 30.0%
GGTGTGTATCAAATTACAAA+AGG + chr1_4:74113345-74113364 None:intergenic 30.0%
!! AATTTGAGTTGATCTTAGCA+AGG + chr1_4:74113907-74113926 None:intergenic 30.0%
ATTTGACCAAAACTTATGAG+AGG - chr1_4:74113924-74113943 Msa0184490:five_prime_UTR 30.0%
TTTGACCAAAACTTATGAGA+GGG - chr1_4:74113925-74113944 Msa0184490:five_prime_UTR 30.0%
!! CGTTTTGAGCTTTTGTTTAC+AGG + chr1_4:74110119-74110138 None:intergenic 35.0%
! AATTAGCTGAAAAGGGGTAT+AGG - chr1_4:74110144-74110163 Msa0184490:three_prime_UTR 35.0%
! ATTAGCTGAAAAGGGGTATA+GGG - chr1_4:74110145-74110164 Msa0184490:three_prime_UTR 35.0%
!! TAGGGTTTTGATCCAAATCT+TGG - chr1_4:74110163-74110182 Msa0184490:three_prime_UTR 35.0%
ATATGGATGGATGGAAGAAA+AGG + chr1_4:74110299-74110318 None:intergenic 35.0%
AAGCTAGAAATATGGATGGA+TGG + chr1_4:74110308-74110327 None:intergenic 35.0%
AGGTCAGAAGAAATTATCGA+AGG - chr1_4:74110373-74110392 Msa0184490:three_prime_UTR 35.0%
!!! GTTTGTTCTCGTTGATTTTG+CGG + chr1_4:74110454-74110473 None:intergenic 35.0%
!! ATTGAAGGGTTTAGGTTGAA+GGG + chr1_4:74110499-74110518 None:intergenic 35.0%
!! AATTGAAGGGTTTAGGTTGA+AGG + chr1_4:74110500-74110519 None:intergenic 35.0%
! GCAATTTTCTTTTACCTGCT+TGG - chr1_4:74110647-74110666 Msa0184490:intron 35.0%
!! TTTTGGATTATTGGCCTGTT+TGG - chr1_4:74110697-74110716 Msa0184490:intron 35.0%
!! CTAGTGGCATAAGTTTTATG+TGG - chr1_4:74110741-74110760 Msa0184490:intron 35.0%
AGACACGTGGAATTAATTGT+GGG + chr1_4:74111129-74111148 None:intergenic 35.0%
AAGACACGTGGAATTAATTG+TGG + chr1_4:74111130-74111149 None:intergenic 35.0%
ATCAAATCCTATTGGTCCAT+GGG + chr1_4:74111218-74111237 None:intergenic 35.0%
AAGGATGTGCTATGCATATT+TGG - chr1_4:74111242-74111261 Msa0184490:intron 35.0%
TCAAACTTGTATCAACGTAC+AGG + chr1_4:74111505-74111524 None:intergenic 35.0%
!! TAAATTTTAGGGGGGTAACA+TGG + chr1_4:74111649-74111668 None:intergenic 35.0%
ATGTAGAAAGAGAGAGAGAA+AGG - chr1_4:74112048-74112067 Msa0184490:intron 35.0%
GAGGAATGAAGATGAAATCA+AGG - chr1_4:74112073-74112092 Msa0184490:intron 35.0%
GAAGATGAAATCAAGGACTT+TGG - chr1_4:74112080-74112099 Msa0184490:intron 35.0%
TCAGTTATACGTAAGACTTG+TGG - chr1_4:74112563-74112582 Msa0184490:intron 35.0%
!!! ATTTTGATTGATGTCGCTCT+TGG - chr1_4:74112886-74112905 Msa0184490:intron 35.0%
!! TTTTGATTGATGTCGCTCTT+GGG - chr1_4:74112887-74112906 Msa0184490:intron 35.0%
! TTCAAACTTCCGAACTGTTT+TGG - chr1_4:74112935-74112954 Msa0184490:intron 35.0%
AATATCTGTCCAAACTGTCA+AGG - chr1_4:74113118-74113137 Msa0184490:intron 35.0%
CACATGATTCTCAATACCAT+TGG - chr1_4:74113262-74113281 Msa0184490:intron 35.0%
CCTGCAAATACAAAAAGATC+AGG + chr1_4:74113586-74113605 None:intergenic 35.0%
! CCTGATCTTTTTGTATTTGC+AGG - chr1_4:74113583-74113602 Msa0184490:intron 35.0%
ATAAACAAAGCAACGTGTAG+TGG - chr1_4:74113650-74113669 Msa0184490:CDS 35.0%
! TGCATTTTGCATCTTCTTCT+AGG - chr1_4:74113681-74113700 Msa0184490:CDS 35.0%
CACTTTGATCAATAGCATAG+AGG + chr1_4:74113991-74114010 None:intergenic 35.0%
ACGACACGAATTAGCTGAAA+AGG - chr1_4:74110136-74110155 Msa0184490:three_prime_UTR 40.0%
CGACACGAATTAGCTGAAAA+GGG - chr1_4:74110137-74110156 Msa0184490:three_prime_UTR 40.0%
GACACGAATTAGCTGAAAAG+GGG - chr1_4:74110138-74110157 Msa0184490:three_prime_UTR 40.0%
AGGTGATAGTACCAGACAAA+TGG - chr1_4:74110393-74110412 Msa0184490:exon 40.0%
GTACCAGACAAATGGAAAGA+AGG - chr1_4:74110401-74110420 Msa0184490:exon 40.0%
AACTCCTTTCCAAGAAGAAC+AGG - chr1_4:74110471-74110490 Msa0184490:intron 40.0%
!!! AATTTTGAGGTGGGGTGTTT+TGG - chr1_4:74110680-74110699 Msa0184490:intron 40.0%
CAGGTAAGTCAAATCCAAAC+AGG + chr1_4:74110714-74110733 None:intergenic 40.0%
ACTTACCTGAGCTTATCTAG+TGG - chr1_4:74110725-74110744 Msa0184490:intron 40.0%
TTATGCCACTAGATAAGCTC+AGG + chr1_4:74110733-74110752 None:intergenic 40.0%
! ACTTGTGCTAGCCTATAAAC+TGG - chr1_4:74110945-74110964 Msa0184490:intron 40.0%
!! CGTGAATTTTAGGCACTGTT+TGG + chr1_4:74110986-74111005 None:intergenic 40.0%
ATGGAACAAAAAGAGTGGCA+TGG + chr1_4:74111030-74111049 None:intergenic 40.0%
! TACAGTTGATTCAAGACACG+TGG + chr1_4:74111103-74111122 None:intergenic 40.0%
! TACAGTTGATTCAAGACACG+TGG + chr1_4:74111103-74111122 None:intergenic 40.0%
!! CTGCCATTTCCTACTTTTGT+GGG + chr1_4:74111168-74111187 None:intergenic 40.0%
!! TCTGCCATTTCCTACTTTTG+TGG + chr1_4:74111169-74111188 None:intergenic 40.0%
ATGGCAGATTTGAATCTGAC+AGG - chr1_4:74111181-74111200 Msa0184490:intron 40.0%
TGGCAGATTTGAATCTGACA+GGG - chr1_4:74111182-74111201 Msa0184490:intron 40.0%
TCAAATCCTATTGGTCCATG+GGG + chr1_4:74111217-74111236 None:intergenic 40.0%
CATCAAATCCTATTGGTCCA+TGG + chr1_4:74111219-74111238 None:intergenic 40.0%
! TTAATGCCATTTACGTGCTG+TGG - chr1_4:74111587-74111606 Msa0184490:intron 40.0%
! AGGACATAAGGTTGACTTCA+TGG - chr1_4:74111702-74111721 Msa0184490:intron 40.0%
! TAAGGTTGACTTCATGGTTG+AGG - chr1_4:74111708-74111727 Msa0184490:intron 40.0%
TAAGGAGTGGAGTGATAAGT+AGG - chr1_4:74111740-74111759 Msa0184490:intron 40.0%
!!! TTACGGGTCGGAGTTTTTTT+TGG - chr1_4:74111958-74111977 Msa0184490:intron 40.0%
AAAGAGAGAGAGAAAGGATG+AGG - chr1_4:74112054-74112073 Msa0184490:intron 40.0%
CAGATCTGAAAGAAGAAGAC+TGG - chr1_4:74112116-74112135 Msa0184490:intron 40.0%
AAGAAGAAGAAGAAGCTGTG+CGG - chr1_4:74112139-74112158 Msa0184490:intron 40.0%
!!! TTTTTTGAGATGCGAGTTGC+AGG - chr1_4:74112507-74112526 Msa0184490:intron 40.0%
TGCTAGTGTTATGTTGTGGA+TGG + chr1_4:74112792-74112811 None:intergenic 40.0%
! TGTCCAAACTGTCAAGGTTT+TGG - chr1_4:74113124-74113143 Msa0184490:intron 40.0%
! GTCCAAACTGTCAAGGTTTT+GGG - chr1_4:74113125-74113144 Msa0184490:intron 40.0%
AACCCAAAACCTTGACAGTT+TGG + chr1_4:74113130-74113149 None:intergenic 40.0%
AGTACATGACACTTGTAGGA+GGG - chr1_4:74113189-74113208 Msa0184490:intron 40.0%
CTGCTTACAAATGATGCACT+TGG + chr1_4:74113446-74113465 None:intergenic 40.0%
ACCAAAACTTATGAGAGGGA+GGG - chr1_4:74113929-74113948 Msa0184490:five_prime_UTR 40.0%
GCACCTTCTTTCCATTTGTC+TGG + chr1_4:74110407-74110426 None:intergenic 45.0%
GGTGCAAGCAATACTACTGA+AGG - chr1_4:74110422-74110441 Msa0184490:CDS 45.0%
GCAAGCAATACTACTGAAGG+CGG - chr1_4:74110425-74110444 Msa0184490:CDS 45.0%
GGAACCTGTTCTTCTTGGAA+AGG + chr1_4:74110478-74110497 None:intergenic 45.0%
TGAAGGGAACCTGTTCTTCT+TGG + chr1_4:74110483-74110502 None:intergenic 45.0%
!! GGTGGGGTGTTTTGGATTAT+TGG - chr1_4:74110688-74110707 Msa0184490:intron 45.0%
! TACCACGCAGCGTGAATTTT+AGG + chr1_4:74110996-74111015 None:intergenic 45.0%
CACGTGGAATTAATTGTGGG+AGG + chr1_4:74111126-74111145 None:intergenic 45.0%
ACTGTACCTCCCACAAAAGT+AGG - chr1_4:74111156-74111175 Msa0184490:intron 45.0%
!! CCATTTCCTACTTTTGTGGG+AGG + chr1_4:74111165-74111184 None:intergenic 45.0%
CCTCCCACAAAAGTAGGAAA+TGG - chr1_4:74111162-74111181 Msa0184490:intron 45.0%
GAGAATCCACAGCACGTAAA+TGG + chr1_4:74111596-74111615 None:intergenic 45.0%
TTAGGGGGGTAACATGGAAA+GGG + chr1_4:74111643-74111662 None:intergenic 45.0%
TTTAGGGGGGTAACATGGAA+AGG + chr1_4:74111644-74111663 None:intergenic 45.0%
! GTTGACTTCATGGTTGAGGT+GGG - chr1_4:74111712-74111731 Msa0184490:intron 45.0%
TTTGACAACAGAGGTGGATG+GGG + chr1_4:74111786-74111805 None:intergenic 45.0%
GTTTGACAACAGAGGTGGAT+GGG + chr1_4:74111787-74111806 None:intergenic 45.0%
! TTTGTGAAAAGCGGGTTTCG+CGG - chr1_4:74111884-74111903 Msa0184490:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG + chr1_4:74112767-74112786 None:intergenic 45.0%
GGATGGCATAGTTGTCAAAG+CGG + chr1_4:74112775-74112794 None:intergenic 45.0%
ACAAGTGTCATGTACTGGAC+AGG + chr1_4:74113185-74113204 None:intergenic 45.0%
GTCCAGTACATGACACTTGT+AGG - chr1_4:74113185-74113204 Msa0184490:intron 45.0%
CTCCTACAAGTGTCATGTAC+TGG + chr1_4:74113190-74113209 None:intergenic 45.0%
CAGTACATGACACTTGTAGG+AGG - chr1_4:74113188-74113207 Msa0184490:intron 45.0%
TGCACTTGGTTGTTCCGTAA+GGG + chr1_4:74113432-74113451 None:intergenic 45.0%
ATGCACTTGGTTGTTCCGTA+AGG + chr1_4:74113433-74113452 None:intergenic 45.0%
CACACGTGTGCTTACTCAAA+AGG - chr1_4:74113493-74113512 Msa0184490:intron 45.0%
GACCAAAACTTATGAGAGGG+AGG - chr1_4:74113928-74113947 Msa0184490:five_prime_UTR 45.0%
CCAAAACTTATGAGAGGGAG+GGG - chr1_4:74113930-74113949 Msa0184490:five_prime_UTR 45.0%
!! GGCGTTTTGGTAGATGAATC+CGG - chr1_4:74113951-74113970 Msa0184490:five_prime_UTR 45.0%
!! TAAAAAAATAAATAATTGAA+GGG + chr1_4:74110513-74110532 None:intergenic 5.0%
!! TTAAAAAAATAAATAATTGA+AGG + chr1_4:74110514-74110533 None:intergenic 5.0%
AGAATCCTTAGCGACTGCGA+TGG - chr1_4:74110248-74110267 Msa0184490:three_prime_UTR 50.0%
AGCAATACTACTGAAGGCGG+TGG - chr1_4:74110428-74110447 Msa0184490:CDS 50.0%
TGCCTAAAATTCACGCTGCG+TGG - chr1_4:74110991-74111010 Msa0184490:intron 50.0%
GATTCTCCCCATGGACCAAT+AGG - chr1_4:74111208-74111227 Msa0184490:intron 50.0%
! GGTTGACTTCATGGTTGAGG+TGG - chr1_4:74111711-74111730 Msa0184490:intron 50.0%
ACTTCATGGTTGAGGTGGGA+GGG - chr1_4:74111716-74111735 Msa0184490:intron 50.0%
CGTTTGACAACAGAGGTGGA+TGG + chr1_4:74111788-74111807 None:intergenic 50.0%
TCCACCTCTGTTGTCAAACG+CGG - chr1_4:74111788-74111807 Msa0184490:intron 50.0%
! CTTTTCACAAACGCGCGAAG+CGG + chr1_4:74111875-74111894 None:intergenic 50.0%
CTTCGCGCGTTTGTGAAAAG+CGG - chr1_4:74111875-74111894 Msa0184490:intron 50.0%
TTCGCGCGTTTGTGAAAAGC+GGG - chr1_4:74111876-74111895 Msa0184490:intron 50.0%
AGCTGTGCGGCTATCAAACT+AGG - chr1_4:74112152-74112171 Msa0184490:intron 50.0%
! TACCGCACCGCGTCAATTTT+TGG - chr1_4:74112726-74112745 Msa0184490:intron 50.0%
! ACCGCACCGCGTCAATTTTT+GGG - chr1_4:74112727-74112746 Msa0184490:intron 50.0%
CCTTGTAGATGGACCCCTTA+CGG - chr1_4:74113415-74113434 Msa0184490:intron 50.0%
GCACTTGGTTGTTCCGTAAG+GGG + chr1_4:74113431-74113450 None:intergenic 50.0%
TTGTAAGCAGCAAGTGCACC+AGG - chr1_4:74113456-74113475 Msa0184490:intron 50.0%
TGTGACAGTACTTGCCATCC+TGG + chr1_4:74113477-74113496 None:intergenic 50.0%
! CCCCTCCCTCTCATAAGTTT+TGG + chr1_4:74113933-74113952 None:intergenic 50.0%
GATGAATCCGGCAAGACGTA+AGG - chr1_4:74113963-74113982 Msa0184490:five_prime_UTR 50.0%
AGAGGAACCTTACGTCTTGC+CGG + chr1_4:74113973-74113992 None:intergenic 50.0%
! GAAGGCTGTCGACGTCGTTT+TGG - chr1_4:74110085-74110104 Msa0184490:three_prime_UTR 55.0%
!!! CTGTCGACGTCGTTTTGGAG+AGG - chr1_4:74110090-74110109 Msa0184490:three_prime_UTR 55.0%
ACAGGGTGAGATTCTCCCCA+TGG - chr1_4:74111199-74111218 Msa0184490:intron 55.0%
GACTTCATGGTTGAGGTGGG+AGG - chr1_4:74111715-74111734 Msa0184490:intron 55.0%
TGGTTGAGGTGGGAGGGTTA+AGG - chr1_4:74111722-74111741 Msa0184490:intron 55.0%
!! CTGACGCGGTAGGCTGATTT+TGG + chr1_4:74111845-74111864 None:intergenic 55.0%
GGTCAAAGCGGGCGCTATTA+CGG - chr1_4:74111941-74111960 Msa0184490:intron 55.0%
GTCAAAGCGGGCGCTATTAC+GGG - chr1_4:74111942-74111961 Msa0184490:intron 55.0%
AATTGACGCGGTGCGGTATG+CGG + chr1_4:74112724-74112743 None:intergenic 55.0%
CCCCAAAAATTGACGCGGTG+CGG + chr1_4:74112731-74112750 None:intergenic 55.0%
! CCGCACCGCGTCAATTTTTG+GGG - chr1_4:74112728-74112747 Msa0184490:intron 55.0%
!! CACCGCGTCAATTTTTGGGG+TGG - chr1_4:74112731-74112750 Msa0184490:intron 55.0%
GGCCACCCCAAAAATTGACG+CGG + chr1_4:74112736-74112755 None:intergenic 55.0%
CCGTAAGGGGTCCATCTACA+AGG + chr1_4:74113418-74113437 None:intergenic 55.0%
AAGCAGCAAGTGCACCAGGA+TGG - chr1_4:74113460-74113479 Msa0184490:intron 55.0%
!!! TATGAGAGGGAGGGGCGTTT+TGG - chr1_4:74113938-74113957 Msa0184490:five_prime_UTR 55.0%
GCAGACCATCGCAGTCGCTA+AGG + chr1_4:74110256-74110275 None:intergenic 60.0%
GAGGTGGGAGGGTTAAGGAG+TGG - chr1_4:74111727-74111746 Msa0184490:intron 60.0%
GCCGCGTTTGACAACAGAGG+TGG + chr1_4:74111792-74111811 None:intergenic 60.0%
TTCACAAACGCGCGAAGCGG+TGG + chr1_4:74111872-74111891 None:intergenic 60.0%
!!! GTTTCGCGGCCGCTTTTACG+TGG - chr1_4:74111898-74111917 Msa0184490:intron 60.0%
CGCTCCGCTCCACGTAAAAG+CGG + chr1_4:74111910-74111929 None:intergenic 60.0%
! CGCTTTTACGTGGAGCGGAG+CGG - chr1_4:74111908-74111927 Msa0184490:intron 60.0%
! GCTTTTACGTGGAGCGGAGC+GGG - chr1_4:74111909-74111928 Msa0184490:intron 60.0%
!! GGGTCGATAGCGGGTCAAAG+CGG - chr1_4:74111929-74111948 Msa0184490:intron 60.0%
!! GGTCGATAGCGGGTCAAAGC+GGG - chr1_4:74111930-74111949 Msa0184490:intron 60.0%
AAGCGGGCGCTATTACGGGT+CGG - chr1_4:74111946-74111965 Msa0184490:intron 60.0%
GCGGGGGCTATTACATGACG+CGG + chr1_4:74112694-74112713 None:intergenic 60.0%
ATTGACGCGGTGCGGTATGC+GGG + chr1_4:74112723-74112742 None:intergenic 60.0%
GCAGGAGTCTGTGCGATGTG+TGG - chr1_4:74113601-74113620 Msa0184490:CDS 60.0%
GCGGCCGCGTTTGACAACAG+AGG + chr1_4:74111795-74111814 None:intergenic 65.0%
TTGTCAAACGCGGCCGCTCC+AGG - chr1_4:74111798-74111817 Msa0184490:intron 65.0%
GAAGCGGTGGCGTTCTGACG+CGG + chr1_4:74111859-74111878 None:intergenic 65.0%
!!! GCGGCCGCTTTTACGTGGAG+CGG - chr1_4:74111903-74111922 Msa0184490:intron 65.0%
GTCAAAGCGGAAACGCGGCG+CGG + chr1_4:74112762-74112781 None:intergenic 65.0%
CGGTGGCGTTCTGACGCGGT+AGG + chr1_4:74111855-74111874 None:intergenic 70.0%
GGAGCGGAGCGGGTCGATAG+CGG - chr1_4:74111919-74111938 Msa0184490:intron 70.0%
GAGCGGAGCGGGTCGATAGC+GGG - chr1_4:74111920-74111939 Msa0184490:intron 70.0%
GGCCGCTCCAGGCGCTATCG+CGG - chr1_4:74111809-74111828 Msa0184490:intron 75.0%
CGCCGCGATAGCGCCTGGAG+CGG + chr1_4:74111814-74111833 None:intergenic 75.0%
CTCCAGGCGCTATCGCGGCG+CGG - chr1_4:74111814-74111833 Msa0184490:intron 75.0%
TGCGGTATGCGGGCGCGACG+CGG + chr1_4:74112713-74112732 None:intergenic 75.0%
! CTCCGCGCCGCGATAGCGCC+TGG + chr1_4:74111819-74111838 None:intergenic 80.0%
! GGCGCTATCGCGGCGCGGAG+CGG - chr1_4:74111819-74111838 Msa0184490:intron 80.0%
! GGTATGCGGGCGCGACGCGG+GGG + chr1_4:74112710-74112729 None:intergenic 80.0%
! CGGTATGCGGGCGCGACGCG+GGG + chr1_4:74112711-74112730 None:intergenic 80.0%
! GCGGTATGCGGGCGCGACGC+GGG + chr1_4:74112712-74112731 None:intergenic 80.0%
! AGCGGAAACGCGGCGCGGCG+CGG + chr1_4:74112757-74112776 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74110050 74114029 74110050 ID=Msa0184490;Name=Msa0184490
chr1_4 mRNA 74110050 74114029 74110050 ID=Msa0184490-mRNA-1;Parent=Msa0184490;Name=Msa0184490-mRNA-1;_AED=0.01;_eAED=0.01;_QI=197|1|1|1|0.25|0.6|5|360|96
chr1_4 exon 74110050 74110474 74110050 ID=Msa0184490-mRNA-1:exon:25254;Parent=Msa0184490-mRNA-1
chr1_4 exon 74110565 74110656 74110565 ID=Msa0184490-mRNA-1:exon:25253;Parent=Msa0184490-mRNA-1
chr1_4 exon 74113587 74113704 74113587 ID=Msa0184490-mRNA-1:exon:25252;Parent=Msa0184490-mRNA-1
chr1_4 exon 74113797 74113841 74113797 ID=Msa0184490-mRNA-1:exon:25251;Parent=Msa0184490-mRNA-1
chr1_4 exon 74113862 74114029 74113862 ID=Msa0184490-mRNA-1:exon:25250;Parent=Msa0184490-mRNA-1
chr1_4 five_prime_UTR 74113862 74114029 74113862 ID=Msa0184490-mRNA-1:five_prime_utr;Parent=Msa0184490-mRNA-1
chr1_4 five_prime_UTR 74113813 74113841 74113813 ID=Msa0184490-mRNA-1:five_prime_utr;Parent=Msa0184490-mRNA-1
chr1_4 CDS 74113797 74113812 74113797 ID=Msa0184490-mRNA-1:cds;Parent=Msa0184490-mRNA-1
chr1_4 CDS 74113587 74113704 74113587 ID=Msa0184490-mRNA-1:cds;Parent=Msa0184490-mRNA-1
chr1_4 CDS 74110565 74110656 74110565 ID=Msa0184490-mRNA-1:cds;Parent=Msa0184490-mRNA-1
chr1_4 CDS 74110410 74110474 74110410 ID=Msa0184490-mRNA-1:cds;Parent=Msa0184490-mRNA-1
chr1_4 three_prime_UTR 74110050 74110409 74110050 ID=Msa0184490-mRNA-1:three_prime_utr;Parent=Msa0184490-mRNA-1
Gene Sequence

>Msa0184490

ATGGTCTGCGAAAAGTGTCAGAAGAAATTATCGAAGGTGATAGTACCAGACAAATGGAAAGAAGGTGCAAGCAATACTACTGAAGGCGGTGGCCGCAAAATCAACGAGAACAAACTCCTTTCCAAGAAGAACAGATGGACCCCTTACGGAACAACCAAGTGCATCATTTGTAAGCAGCAAGTGCACCAGGATGGCAAGTACTGTCACACGTGTGCTTACTCAAAAGGAGTCTGTGCGATGTGTGGCAAGCAAGTTCTTGACACCAAGTTTTATAAACAAAGCAACGTGTAG
Protein sequence

>Msa0184490

MVCEKCQKKLSKVIVPDKWKEGASNTTEGGGRKINENKLLSKKNRWTPYGTTKCIICKQQVHQDGKYCHTCAYSKGVCAMCGKQVLDTKFYKQSNV*