AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1145290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1145290 MtrunA17_Chr1g0206091 100.000 96 0 0 1 96 1 96 1.73e-67 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1145290 AT1G61780.1 88.776 98 9 1 1 96 1 98 7.77e-60 178

Find 45 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCTTGGATATTCAAAT+TGG 0.227939 7_4:-21985542 Msa1145290:five_prime_UTR
CAATTTGAATATCCAAGATT+TGG 0.257745 7_4:+21985543 None:intergenic
TGAAGGGAACCTGTTCTTCT+TGG 0.293706 7_4:+21985238 None:intergenic
CCCCTCCCTCTCATAAGTTT+TGG 0.330996 7_4:+21981791 None:intergenic
AATTAGCTGAAAAGGGGTAT+AGG 0.350950 7_4:-21985574 Msa1145290:five_prime_UTR
GAAGGCTGTCGACGTCGTTT+TGG 0.355325 7_4:-21985633 Msa1145290:five_prime_UTR
GCACCTTCTTTCCATTTGTC+TGG 0.356085 7_4:+21985314 None:intergenic
ATTAGCTGAAAAGGGGTATA+GGG 0.371633 7_4:-21985573 Msa1145290:five_prime_UTR
TTAGACTATATTATATATAA+AGG 0.375911 7_4:+21982003 None:intergenic
TATGAGAGGGAGGGGCGTTT+TGG 0.376336 7_4:-21981783 Msa1145290:three_prime_UTR
AACTCCTTTCCAAGAAGAAC+AGG 0.405714 7_4:-21985247 Msa1145290:intron
ACGACACGAATTAGCTGAAA+AGG 0.416736 7_4:-21985582 Msa1145290:five_prime_UTR
AGAGGAACCTTACGTCTTGC+CGG 0.429446 7_4:+21981751 None:intergenic
CCTTGTAGATGGACCCCTTA+CGG 0.465158 7_4:-21982306 Msa1145290:intron
GTTGCTTTGTTTATAAAACT+TGG 0.466176 7_4:+21982080 None:intergenic
AGGTGATAGTACCAGACAAA+TGG 0.467879 7_4:-21985325 Msa1145290:CDS
CACACGTGTGCTTACTCAAA+AGG 0.468563 7_4:-21982228 Msa1145290:intron
TGCACTTGGTTGTTCCGTAA+GGG 0.477894 7_4:+21982292 None:intergenic
ATGCACTTGGTTGTTCCGTA+AGG 0.478636 7_4:+21982291 None:intergenic
CTGCTTACAAATGATGCACT+TGG 0.484104 7_4:+21982278 None:intergenic
CGACACGAATTAGCTGAAAA+GGG 0.490307 7_4:-21985581 Msa1145290:five_prime_UTR
CACTTTGATCAATAGCATAG+AGG 0.500824 7_4:+21981733 None:intergenic
ATAAACAAAGCAACGTGTAG+TGG 0.523993 7_4:-21982071 Msa1145290:exon
TTGTAAGCAGCAAGTGCACC+AGG 0.524349 7_4:-21982265 Msa1145290:CDS
GCAGACCATCGCAGTCGCTA+AGG 0.528087 7_4:+21985465 None:intergenic
CCAAAACTTATGAGAGGGAG+GGG 0.533985 7_4:-21981791 Msa1145290:three_prime_UTR
GCACTTGGTTGTTCCGTAAG+GGG 0.542962 7_4:+21982293 None:intergenic
TGTGACAGTACTTGCCATCC+TGG 0.544753 7_4:+21982247 None:intergenic
GGAACCTGTTCTTCTTGGAA+AGG 0.545948 7_4:+21985243 None:intergenic
GATGAATCCGGCAAGACGTA+AGG 0.551859 7_4:-21981758 Msa1145290:three_prime_UTR
GTACCAGACAAATGGAAAGA+AGG 0.553949 7_4:-21985317 Msa1145290:CDS
ACCAAAACTTATGAGAGGGA+GGG 0.578094 7_4:-21981792 Msa1145290:three_prime_UTR
TTTGACCAAAACTTATGAGA+GGG 0.582502 7_4:-21981796 Msa1145290:three_prime_UTR
GACCAAAACTTATGAGAGGG+AGG 0.596320 7_4:-21981793 Msa1145290:three_prime_UTR
TAGATCTCATAAATACTCGA+AGG 0.603857 7_4:-21985651 Msa1145290:five_prime_UTR
ATTTGACCAAAACTTATGAG+AGG 0.607189 7_4:-21981797 Msa1145290:three_prime_UTR
GGTGCAAGCAATACTACTGA+AGG 0.608421 7_4:-21985296 Msa1145290:CDS
AGCAATACTACTGAAGGCGG+TGG 0.615667 7_4:-21985290 Msa1145290:CDS
AATTTGAGTTGATCTTAGCA+AGG 0.619178 7_4:+21981817 None:intergenic
AGAATCCTTAGCGACTGCGA+TGG 0.623608 7_4:-21985470 Msa1145290:exon
AAGCAGCAAGTGCACCAGGA+TGG 0.635149 7_4:-21982261 Msa1145290:CDS
GCAGGAGTCTGTGCGATGTG+TGG 0.652505 7_4:-21982120 Msa1145290:intron
GACACGAATTAGCTGAAAAG+GGG 0.659143 7_4:-21985580 Msa1145290:five_prime_UTR
GCAAGCAATACTACTGAAGG+CGG 0.661649 7_4:-21985293 Msa1145290:CDS
AGGTCAGAAGAAATTATCGA+AGG 0.666813 7_4:-21985345 Msa1145290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTATTTTTTTAATT+AGG - chr7_4:21982180-21982199 Msa1145290:intron 0.0%
!!! ATTATTTATTTTTTTAATTA+GGG - chr7_4:21982181-21982200 Msa1145290:intron 0.0%
!!! AATAATTTTAGTTTTGATAT+TGG - chr7_4:21982276-21982295 Msa1145290:CDS 10.0%
!!! AAAAAGTAAATAAATTTTAG+GGG + chr7_4:21983323-21983342 None:intergenic 10.0%
!!! GAAAAAGTAAATAAATTTTA+GGG + chr7_4:21983324-21983343 None:intergenic 10.0%
!!! TGAAAAAGTAAATAAATTTT+AGG + chr7_4:21983325-21983344 None:intergenic 10.0%
!!! TAAATTTTGAGTAATATTTA+AGG - chr7_4:21984274-21984293 Msa1145290:intron 10.0%
!!! AATATATTTTTTGTATAGTA+TGG - chr7_4:21984303-21984322 Msa1145290:intron 10.0%
!! TTAGACTATATTATATATAA+AGG + chr7_4:21985382-21985401 None:intergenic 10.0%
!!! GTAAATTCAATTGAATTTTT+TGG - chr7_4:21981994-21982013 Msa1145290:three_prime_UTR 15.0%
!!! AAAAGTAAATAAATTTTAGG+GGG + chr7_4:21983322-21983341 None:intergenic 15.0%
!! AAAAAGATAAGTAAAAAGAA+CGG + chr7_4:21984015-21984034 None:intergenic 15.0%
!! ATTTACAAAAGCTAGAAATA+TGG + chr7_4:21981980-21981999 None:intergenic 20.0%
!! AATAAATAATTGAAGGGTTT+AGG + chr7_4:21982171-21982190 None:intergenic 20.0%
!!! TATTTGGTCTGTTTTTTTTT+TGG - chr7_4:21982922-21982941 Msa1145290:intron 20.0%
!!! TTTTATATGTAGACTTGATA+TGG - chr7_4:21982991-21983010 Msa1145290:intron 20.0%
!!! GTTTGAAAACAGTTATTTTT+TGG - chr7_4:21983183-21983202 Msa1145290:intron 20.0%
!!! AAAGTAAATAAATTTTAGGG+GGG + chr7_4:21983321-21983340 None:intergenic 20.0%
!!! CCTGTTAAAAAATAAAGTTT+TGG + chr7_4:21983349-21983368 None:intergenic 20.0%
!!! CCAAAACTTTATTTTTTAAC+AGG - chr7_4:21983346-21983365 Msa1145290:intron 20.0%
!!! TTATTTTTTAACAGGACATA+AGG - chr7_4:21983354-21983373 Msa1145290:intron 20.0%
!!! TTTTTTTTGGTTGATTTTGT+GGG - chr7_4:21983635-21983654 Msa1145290:intron 20.0%
!! AAAGGAAAACATTTAGTATT+GGG + chr7_4:21983857-21983876 None:intergenic 20.0%
!! AAAAGGAAAACATTTAGTAT+TGG + chr7_4:21983858-21983877 None:intergenic 20.0%
!!! TATTTTTCTTTTTGAAAGAC+AGG + chr7_4:21983914-21983933 None:intergenic 20.0%
!! AATAATCTAAGTAGAAAGAA+AGG + chr7_4:21983966-21983985 None:intergenic 20.0%
!! AAGCTATTGAACATTAAAAT+TGG - chr7_4:21984510-21984529 Msa1145290:intron 20.0%
!!! ATATTTTTGATATATCTGAG+TGG - chr7_4:21984573-21984592 Msa1145290:intron 20.0%
!! TGAACATTTACAAAAAGTAA+TGG + chr7_4:21984881-21984900 None:intergenic 20.0%
!! CTGTAAAAAGTTAATATTGT+GGG - chr7_4:21985432-21985451 Msa1145290:intron 20.0%
! CAATTTGAATATCCAAGATT+TGG + chr7_4:21981842-21981861 None:intergenic 25.0%
! CAAATCTTGGATATTCAAAT+TGG - chr7_4:21981840-21981859 Msa1145290:three_prime_UTR 25.0%
! TAATTTGAATTCGATGTTGT+AGG - chr7_4:21982017-21982036 Msa1145290:three_prime_UTR 25.0%
!! GTATGAAAAGCATAGTAATT+TGG - chr7_4:21982203-21982222 Msa1145290:intron 25.0%
!! TGGTATAACTTCAAATTTTG+AGG - chr7_4:21982331-21982350 Msa1145290:intron 25.0%
!!! TATAACTTCAAATTTTGAGG+TGG - chr7_4:21982334-21982353 Msa1145290:intron 25.0%
!!! ATAACTTCAAATTTTGAGGT+GGG - chr7_4:21982335-21982354 Msa1145290:intron 25.0%
!!! ATAAGTTTTATGTGGTTGTT+CGG - chr7_4:21982413-21982432 Msa1145290:intron 25.0%
! TAGCTTATGATATTGTTCAT+AGG - chr7_4:21982451-21982470 Msa1145290:intron 25.0%
! TTAAGATAGCTTATGAAAAC+AGG - chr7_4:21982502-21982521 Msa1145290:intron 25.0%
! AAAAAATGGAACAAAAAGAG+TGG + chr7_4:21982699-21982718 None:intergenic 25.0%
!! AGCATCGAGTTTAAAAAAAA+TGG + chr7_4:21982713-21982732 None:intergenic 25.0%
!! GAAAGAAACCTTATGTTTTT+AGG - chr7_4:21983036-21983055 Msa1145290:intron 25.0%
! AATAAAATGCAAGGGAAAAA+CGG + chr7_4:21983232-21983251 None:intergenic 25.0%
! TGGCATTAAATAAAATGCAA+GGG + chr7_4:21983240-21983259 None:intergenic 25.0%
! ATGGCATTAAATAAAATGCA+AGG + chr7_4:21983241-21983260 None:intergenic 25.0%
!!! GTTTTTTTTGGTTGATTTTG+TGG - chr7_4:21983634-21983653 Msa1145290:intron 25.0%
!!! AGAGTAAGGTGTTTTATAAA+AGG + chr7_4:21983875-21983894 None:intergenic 25.0%
!!! AATAGCTTTCTTTTTTGGAA+TGG + chr7_4:21984498-21984517 None:intergenic 25.0%
!!! TGTTCAATAGCTTTCTTTTT+TGG + chr7_4:21984503-21984522 None:intergenic 25.0%
! AGACATATTATGATGAGATA+TGG + chr7_4:21984644-21984663 None:intergenic 25.0%
!!! TGTAATTCTTGCTTTTTGAA+TGG - chr7_4:21984753-21984772 Msa1145290:intron 25.0%
! ACAGATCAAAGTATAGTAAT+TGG + chr7_4:21984961-21984980 None:intergenic 25.0%
! CAAATTACAAAAGGAGAAAA+GGG + chr7_4:21984997-21985016 None:intergenic 25.0%
! TCAAATTACAAAAGGAGAAA+AGG + chr7_4:21984998-21985017 None:intergenic 25.0%
! GTTCACATAACATAAAAACA+GGG + chr7_4:21985027-21985046 None:intergenic 25.0%
! TGTTCACATAACATAAAAAC+AGG + chr7_4:21985028-21985047 None:intergenic 25.0%
! TTGAATTTATTCCTTGTAGA+TGG - chr7_4:21985065-21985084 Msa1145290:intron 25.0%
!!! GTTGCTTTGTTTATAAAACT+TGG + chr7_4:21985305-21985324 None:intergenic 25.0%
! GCTGTAAAAAGTTAATATTG+TGG - chr7_4:21985431-21985450 Msa1145290:intron 25.0%
!!! ACAATAGTGTTTTTTCGATA+AGG + chr7_4:21985493-21985512 None:intergenic 25.0%
! TATCGAAAAAACACTATTGT+TGG - chr7_4:21985493-21985512 Msa1145290:intron 25.0%
TAGATCTCATAAATACTCGA+AGG - chr7_4:21981731-21981750 Msa1145290:three_prime_UTR 30.0%
ACAAAAGCTAGAAATATGGA+TGG + chr7_4:21981976-21981995 None:intergenic 30.0%
!! AGTTTTGATATTGGAAGCTT+TGG - chr7_4:21982285-21982304 Msa1145290:CDS 30.0%
!! GTTTTGATATTGGAAGCTTT+GGG - chr7_4:21982286-21982305 Msa1145290:CDS 30.0%
! AATTTGAAGTTATACCAAGC+AGG + chr7_4:21982328-21982347 None:intergenic 30.0%
!!! TAACTTCAAATTTTGAGGTG+GGG - chr7_4:21982336-21982355 Msa1145290:intron 30.0%
!! TAAGTTTTATGTGGTTGTTC+GGG - chr7_4:21982414-21982433 Msa1145290:intron 30.0%
! ACAACTTGTTTCCAGTTTAT+AGG + chr7_4:21982623-21982642 None:intergenic 30.0%
CCTTAAACATCAAATCCTAT+TGG + chr7_4:21982890-21982909 None:intergenic 30.0%
CCAATAGGATTTGATGTTTA+AGG - chr7_4:21982887-21982906 Msa1145290:intron 30.0%
! ACTTGATATGGTTAGTTGTA+TGG - chr7_4:21983003-21983022 Msa1145290:intron 30.0%
!!! AGAAACCTTATGTTTTTAGG+TGG - chr7_4:21983039-21983058 Msa1145290:intron 30.0%
AACTACCACCTAAAAACATA+AGG + chr7_4:21983047-21983066 None:intergenic 30.0%
TGAACAAGTGAATCAATTGT+TGG - chr7_4:21983106-21983125 Msa1145290:intron 30.0%
AATCAATTGTTGGATGTCAT+TGG - chr7_4:21983116-21983135 Msa1145290:intron 30.0%
ATCAATTGTTGGATGTCATT+GGG - chr7_4:21983117-21983136 Msa1145290:intron 30.0%
AGTGAGCATGATGAAATTAT+GGG + chr7_4:21983282-21983301 None:intergenic 30.0%
AAGTGAGCATGATGAAATTA+TGG + chr7_4:21983283-21983302 None:intergenic 30.0%
! TCTCGTTTTATATGCGTTTT+TGG + chr7_4:21983681-21983700 None:intergenic 30.0%
ATCAAACTAGGTTAAAGTTG+TGG - chr7_4:21983828-21983847 Msa1145290:intron 30.0%
GGTGTATTAGTAAAAGAGTA+AGG + chr7_4:21983889-21983908 None:intergenic 30.0%
!! TTTCTTTTTGAAAGACAGGT+GGG + chr7_4:21983910-21983929 None:intergenic 30.0%
!! TTTTCTTTTTGAAAGACAGG+TGG + chr7_4:21983911-21983930 None:intergenic 30.0%
TATATGCTAGTGTTATGTTG+TGG + chr7_4:21984457-21984476 None:intergenic 30.0%
TGGAATGGAAAATGTTAGTA+TGG + chr7_4:21984483-21984502 None:intergenic 30.0%
ACAATGTAACCAAAACAGTT+CGG + chr7_4:21984608-21984627 None:intergenic 30.0%
GCGCTTATATTTAGTAATCA+CGG - chr7_4:21984723-21984742 Msa1145290:intron 30.0%
! GTTTAGAATGAGTGATGTTA+TGG - chr7_4:21984808-21984827 Msa1145290:intron 30.0%
TTGGATAAAAAACTTGCCAA+TGG + chr7_4:21984942-21984961 None:intergenic 30.0%
!! ACTTTGATCTGTTGTTTCAA+TGG - chr7_4:21984969-21984988 Msa1145290:intron 30.0%
!! CTTTGATCTGTTGTTTCAAT+GGG - chr7_4:21984970-21984989 Msa1145290:intron 30.0%
GGTGTGTATCAAATTACAAA+AGG + chr7_4:21985006-21985025 None:intergenic 30.0%
!! AATTTGAGTTGATCTTAGCA+AGG + chr7_4:21985568-21985587 None:intergenic 30.0%
ATTTGACCAAAACTTATGAG+AGG - chr7_4:21985585-21985604 Msa1145290:five_prime_UTR 30.0%
TTTGACCAAAACTTATGAGA+GGG - chr7_4:21985586-21985605 Msa1145290:five_prime_UTR 30.0%
!! CGTTTTGAGCTTTTGTTTAC+AGG + chr7_4:21981783-21981802 None:intergenic 35.0%
! AATTAGCTGAAAAGGGGTAT+AGG - chr7_4:21981808-21981827 Msa1145290:three_prime_UTR 35.0%
! ATTAGCTGAAAAGGGGTATA+GGG - chr7_4:21981809-21981828 Msa1145290:three_prime_UTR 35.0%
!! TAGGGTTTTGATCCAAATCT+TGG - chr7_4:21981827-21981846 Msa1145290:three_prime_UTR 35.0%
ATATGGATGGATGGAAGAAA+AGG + chr7_4:21981963-21981982 None:intergenic 35.0%
AAGCTAGAAATATGGATGGA+TGG + chr7_4:21981972-21981991 None:intergenic 35.0%
AGGTCAGAAGAAATTATCGA+AGG - chr7_4:21982037-21982056 Msa1145290:three_prime_UTR 35.0%
!!! GTTTGTTCTCGTTGATTTTG+CGG + chr7_4:21982118-21982137 None:intergenic 35.0%
!! ATTGAAGGGTTTAGGTTGAA+GGG + chr7_4:21982163-21982182 None:intergenic 35.0%
!! AATTGAAGGGTTTAGGTTGA+AGG + chr7_4:21982164-21982183 None:intergenic 35.0%
! GCAATTTTCTTTTACCTGCT+TGG - chr7_4:21982311-21982330 Msa1145290:intron 35.0%
!! TTTTGGATTATTGGCCTGTT+TGG - chr7_4:21982361-21982380 Msa1145290:intron 35.0%
!! CTAGTGGCATAAGTTTTATG+TGG - chr7_4:21982405-21982424 Msa1145290:intron 35.0%
AGACACGTGGAATTAATTGT+GGG + chr7_4:21982793-21982812 None:intergenic 35.0%
AAGACACGTGGAATTAATTG+TGG + chr7_4:21982794-21982813 None:intergenic 35.0%
ATCAAATCCTATTGGTCCAT+GGG + chr7_4:21982882-21982901 None:intergenic 35.0%
AAGGATGTGCTATGCATATT+TGG - chr7_4:21982906-21982925 Msa1145290:intron 35.0%
TCAAACTTGTATCAACGTAC+AGG + chr7_4:21983169-21983188 None:intergenic 35.0%
!! TAAATTTTAGGGGGGTAACA+TGG + chr7_4:21983313-21983332 None:intergenic 35.0%
ATGTAGAAAGAGAGAGAGAA+AGG - chr7_4:21983712-21983731 Msa1145290:intron 35.0%
GAGGAATGAAGATGAAATCA+AGG - chr7_4:21983737-21983756 Msa1145290:intron 35.0%
GAAGATGAAATCAAGGACTT+TGG - chr7_4:21983744-21983763 Msa1145290:intron 35.0%
TCAGTTATACGTAAGACTTG+TGG - chr7_4:21984224-21984243 Msa1145290:intron 35.0%
!!! ATTTTGATTGATGTCGCTCT+TGG - chr7_4:21984547-21984566 Msa1145290:intron 35.0%
!! TTTTGATTGATGTCGCTCTT+GGG - chr7_4:21984548-21984567 Msa1145290:intron 35.0%
! TTCAAACTTCCGAACTGTTT+TGG - chr7_4:21984596-21984615 Msa1145290:intron 35.0%
AATATCTGTCCAAACTGTCA+AGG - chr7_4:21984779-21984798 Msa1145290:intron 35.0%
CACATGATTCTCAATACCAT+TGG - chr7_4:21984923-21984942 Msa1145290:intron 35.0%
CCTGCAAATACAAAAAGATC+AGG + chr7_4:21985247-21985266 None:intergenic 35.0%
! CCTGATCTTTTTGTATTTGC+AGG - chr7_4:21985244-21985263 Msa1145290:intron 35.0%
ATAAACAAAGCAACGTGTAG+TGG - chr7_4:21985311-21985330 Msa1145290:CDS 35.0%
! TGCATTTTGCATCTTCTTCT+AGG - chr7_4:21985342-21985361 Msa1145290:CDS 35.0%
CACTTTGATCAATAGCATAG+AGG + chr7_4:21985652-21985671 None:intergenic 35.0%
ACGACACGAATTAGCTGAAA+AGG - chr7_4:21981800-21981819 Msa1145290:three_prime_UTR 40.0%
CGACACGAATTAGCTGAAAA+GGG - chr7_4:21981801-21981820 Msa1145290:three_prime_UTR 40.0%
GACACGAATTAGCTGAAAAG+GGG - chr7_4:21981802-21981821 Msa1145290:three_prime_UTR 40.0%
AGGTGATAGTACCAGACAAA+TGG - chr7_4:21982057-21982076 Msa1145290:exon 40.0%
GTACCAGACAAATGGAAAGA+AGG - chr7_4:21982065-21982084 Msa1145290:exon 40.0%
AACTCCTTTCCAAGAAGAAC+AGG - chr7_4:21982135-21982154 Msa1145290:intron 40.0%
!!! AATTTTGAGGTGGGGTGTTT+TGG - chr7_4:21982344-21982363 Msa1145290:intron 40.0%
CAGGTAAGTCAAATCCAAAC+AGG + chr7_4:21982378-21982397 None:intergenic 40.0%
ACTTACCTGAGCTTATCTAG+TGG - chr7_4:21982389-21982408 Msa1145290:intron 40.0%
TTATGCCACTAGATAAGCTC+AGG + chr7_4:21982397-21982416 None:intergenic 40.0%
! ACTTGTGCTAGCCTATAAAC+TGG - chr7_4:21982609-21982628 Msa1145290:intron 40.0%
!! CGTGAATTTTAGGCACTGTT+TGG + chr7_4:21982650-21982669 None:intergenic 40.0%
ATGGAACAAAAAGAGTGGCA+TGG + chr7_4:21982694-21982713 None:intergenic 40.0%
! TACAGTTGATTCAAGACACG+TGG + chr7_4:21982767-21982786 None:intergenic 40.0%
! TACAGTTGATTCAAGACACG+TGG + chr7_4:21982767-21982786 None:intergenic 40.0%
!! CTGCCATTTCCTACTTTTGT+GGG + chr7_4:21982832-21982851 None:intergenic 40.0%
!! TCTGCCATTTCCTACTTTTG+TGG + chr7_4:21982833-21982852 None:intergenic 40.0%
ATGGCAGATTTGAATCTGAC+AGG - chr7_4:21982845-21982864 Msa1145290:intron 40.0%
TGGCAGATTTGAATCTGACA+GGG - chr7_4:21982846-21982865 Msa1145290:intron 40.0%
TCAAATCCTATTGGTCCATG+GGG + chr7_4:21982881-21982900 None:intergenic 40.0%
CATCAAATCCTATTGGTCCA+TGG + chr7_4:21982883-21982902 None:intergenic 40.0%
! TTAATGCCATTTACGTGCTG+TGG - chr7_4:21983251-21983270 Msa1145290:intron 40.0%
! AGGACATAAGGTTGACTTCA+TGG - chr7_4:21983366-21983385 Msa1145290:intron 40.0%
! TAAGGTTGACTTCATGGTTG+AGG - chr7_4:21983372-21983391 Msa1145290:intron 40.0%
TAAGGAGTGGAGTGATAAGT+AGG - chr7_4:21983404-21983423 Msa1145290:intron 40.0%
!!! TTACGGGTCGGAGTTTTTTT+TGG - chr7_4:21983622-21983641 Msa1145290:intron 40.0%
AAAGAGAGAGAGAAAGGATG+AGG - chr7_4:21983718-21983737 Msa1145290:intron 40.0%
CAGATCTGAAAGAAGAAGAC+TGG - chr7_4:21983780-21983799 Msa1145290:intron 40.0%
AAGAAGAAGAAGAAGCTGTG+CGG - chr7_4:21983803-21983822 Msa1145290:intron 40.0%
TGCTAGTGTTATGTTGTGGA+TGG + chr7_4:21984453-21984472 None:intergenic 40.0%
! TGTCCAAACTGTCAAGGTTT+TGG - chr7_4:21984785-21984804 Msa1145290:intron 40.0%
! GTCCAAACTGTCAAGGTTTT+GGG - chr7_4:21984786-21984805 Msa1145290:intron 40.0%
AACCCAAAACCTTGACAGTT+TGG + chr7_4:21984791-21984810 None:intergenic 40.0%
AGTACATGACACTTGTAGGA+GGG - chr7_4:21984850-21984869 Msa1145290:intron 40.0%
CTGCTTACAAATGATGCACT+TGG + chr7_4:21985107-21985126 None:intergenic 40.0%
ACCAAAACTTATGAGAGGGA+GGG - chr7_4:21985590-21985609 Msa1145290:five_prime_UTR 40.0%
GCACCTTCTTTCCATTTGTC+TGG + chr7_4:21982071-21982090 None:intergenic 45.0%
GGTGCAAGCAATACTACTGA+AGG - chr7_4:21982086-21982105 Msa1145290:CDS 45.0%
GCAAGCAATACTACTGAAGG+CGG - chr7_4:21982089-21982108 Msa1145290:CDS 45.0%
GGAACCTGTTCTTCTTGGAA+AGG + chr7_4:21982142-21982161 None:intergenic 45.0%
TGAAGGGAACCTGTTCTTCT+TGG + chr7_4:21982147-21982166 None:intergenic 45.0%
!! GGTGGGGTGTTTTGGATTAT+TGG - chr7_4:21982352-21982371 Msa1145290:intron 45.0%
! TACCACGCAGCGTGAATTTT+AGG + chr7_4:21982660-21982679 None:intergenic 45.0%
CACGTGGAATTAATTGTGGG+AGG + chr7_4:21982790-21982809 None:intergenic 45.0%
ACTGTACCTCCCACAAAAGT+AGG - chr7_4:21982820-21982839 Msa1145290:intron 45.0%
!! CCATTTCCTACTTTTGTGGG+AGG + chr7_4:21982829-21982848 None:intergenic 45.0%
CCTCCCACAAAAGTAGGAAA+TGG - chr7_4:21982826-21982845 Msa1145290:intron 45.0%
GAGAATCCACAGCACGTAAA+TGG + chr7_4:21983260-21983279 None:intergenic 45.0%
TTAGGGGGGTAACATGGAAA+GGG + chr7_4:21983307-21983326 None:intergenic 45.0%
TTTAGGGGGGTAACATGGAA+AGG + chr7_4:21983308-21983327 None:intergenic 45.0%
! GTTGACTTCATGGTTGAGGT+GGG - chr7_4:21983376-21983395 Msa1145290:intron 45.0%
TTTGACAACAGAGGTGGATG+GGG + chr7_4:21983450-21983469 None:intergenic 45.0%
GTTTGACAACAGAGGTGGAT+GGG + chr7_4:21983451-21983470 None:intergenic 45.0%
! TTTGTGAAAAGCGGGTTTCG+CGG - chr7_4:21983548-21983567 Msa1145290:intron 45.0%
!!! CTTTTTGAGATGCGAGTTGC+AGG - chr7_4:21984168-21984187 Msa1145290:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG + chr7_4:21984428-21984447 None:intergenic 45.0%
GGATGGCATAGTTGTCAAAG+CGG + chr7_4:21984436-21984455 None:intergenic 45.0%
ACAAGTGTCATGTACTGGAC+AGG + chr7_4:21984846-21984865 None:intergenic 45.0%
GTCCAGTACATGACACTTGT+AGG - chr7_4:21984846-21984865 Msa1145290:intron 45.0%
CTCCTACAAGTGTCATGTAC+TGG + chr7_4:21984851-21984870 None:intergenic 45.0%
CAGTACATGACACTTGTAGG+AGG - chr7_4:21984849-21984868 Msa1145290:intron 45.0%
TGCACTTGGTTGTTCCGTAA+GGG + chr7_4:21985093-21985112 None:intergenic 45.0%
ATGCACTTGGTTGTTCCGTA+AGG + chr7_4:21985094-21985113 None:intergenic 45.0%
CACACGTGTGCTTACTCAAA+AGG - chr7_4:21985154-21985173 Msa1145290:intron 45.0%
GACCAAAACTTATGAGAGGG+AGG - chr7_4:21985589-21985608 Msa1145290:five_prime_UTR 45.0%
CCAAAACTTATGAGAGGGAG+GGG - chr7_4:21985591-21985610 Msa1145290:five_prime_UTR 45.0%
!! GGCGTTTTGGTAGATGAATC+CGG - chr7_4:21985612-21985631 Msa1145290:five_prime_UTR 45.0%
!! TAAAAAAATAAATAATTGAA+GGG + chr7_4:21982177-21982196 None:intergenic 5.0%
!! TTAAAAAAATAAATAATTGA+AGG + chr7_4:21982178-21982197 None:intergenic 5.0%
AGAATCCTTAGCGACTGCGA+TGG - chr7_4:21981912-21981931 Msa1145290:three_prime_UTR 50.0%
AGCAATACTACTGAAGGCGG+TGG - chr7_4:21982092-21982111 Msa1145290:CDS 50.0%
TGCCTAAAATTCACGCTGCG+TGG - chr7_4:21982655-21982674 Msa1145290:intron 50.0%
GATTCTCCCCATGGACCAAT+AGG - chr7_4:21982872-21982891 Msa1145290:intron 50.0%
! GGTTGACTTCATGGTTGAGG+TGG - chr7_4:21983375-21983394 Msa1145290:intron 50.0%
ACTTCATGGTTGAGGTGGGA+GGG - chr7_4:21983380-21983399 Msa1145290:intron 50.0%
CGTTTGACAACAGAGGTGGA+TGG + chr7_4:21983452-21983471 None:intergenic 50.0%
TCCACCTCTGTTGTCAAACG+CGG - chr7_4:21983452-21983471 Msa1145290:intron 50.0%
! CTTTTCACAAACGCGCGAAG+CGG + chr7_4:21983539-21983558 None:intergenic 50.0%
CTTCGCGCGTTTGTGAAAAG+CGG - chr7_4:21983539-21983558 Msa1145290:intron 50.0%
TTCGCGCGTTTGTGAAAAGC+GGG - chr7_4:21983540-21983559 Msa1145290:intron 50.0%
AGCTGTGCGGCTATCAAACT+AGG - chr7_4:21983816-21983835 Msa1145290:intron 50.0%
! TACCGCACCGCGTCAATTTT+TGG - chr7_4:21984387-21984406 Msa1145290:intron 50.0%
! ACCGCACCGCGTCAATTTTT+GGG - chr7_4:21984388-21984407 Msa1145290:intron 50.0%
CCTTGTAGATGGACCCCTTA+CGG - chr7_4:21985076-21985095 Msa1145290:intron 50.0%
GCACTTGGTTGTTCCGTAAG+GGG + chr7_4:21985092-21985111 None:intergenic 50.0%
TTGTAAGCAGCAAGTGCACC+AGG - chr7_4:21985117-21985136 Msa1145290:intron 50.0%
TGTGACAGTACTTGCCATCC+TGG + chr7_4:21985138-21985157 None:intergenic 50.0%
! CCCCTCCCTCTCATAAGTTT+TGG + chr7_4:21985594-21985613 None:intergenic 50.0%
GATGAATCCGGCAAGACGTA+AGG - chr7_4:21985624-21985643 Msa1145290:five_prime_UTR 50.0%
AGAGGAACCTTACGTCTTGC+CGG + chr7_4:21985634-21985653 None:intergenic 50.0%
! GAAGGCTGTCGACGTCGTTT+TGG - chr7_4:21981749-21981768 Msa1145290:three_prime_UTR 55.0%
!!! CTGTCGACGTCGTTTTGGAG+AGG - chr7_4:21981754-21981773 Msa1145290:three_prime_UTR 55.0%
ACAGGGTGAGATTCTCCCCA+TGG - chr7_4:21982863-21982882 Msa1145290:intron 55.0%
GACTTCATGGTTGAGGTGGG+AGG - chr7_4:21983379-21983398 Msa1145290:intron 55.0%
TGGTTGAGGTGGGAGGGTTA+AGG - chr7_4:21983386-21983405 Msa1145290:intron 55.0%
!! CTGACGCGGTAGGCTGATTT+TGG + chr7_4:21983509-21983528 None:intergenic 55.0%
GGTCAAAGCGGGCGCTATTA+CGG - chr7_4:21983605-21983624 Msa1145290:intron 55.0%
GTCAAAGCGGGCGCTATTAC+GGG - chr7_4:21983606-21983625 Msa1145290:intron 55.0%
AATTGACGCGGTGCGGTATG+CGG + chr7_4:21984385-21984404 None:intergenic 55.0%
CCCCAAAAATTGACGCGGTG+CGG + chr7_4:21984392-21984411 None:intergenic 55.0%
! CCGCACCGCGTCAATTTTTG+GGG - chr7_4:21984389-21984408 Msa1145290:intron 55.0%
!! CACCGCGTCAATTTTTGGGG+TGG - chr7_4:21984392-21984411 Msa1145290:intron 55.0%
GGCCACCCCAAAAATTGACG+CGG + chr7_4:21984397-21984416 None:intergenic 55.0%
CCGTAAGGGGTCCATCTACA+AGG + chr7_4:21985079-21985098 None:intergenic 55.0%
AAGCAGCAAGTGCACCAGGA+TGG - chr7_4:21985121-21985140 Msa1145290:intron 55.0%
!!! TATGAGAGGGAGGGGCGTTT+TGG - chr7_4:21985599-21985618 Msa1145290:five_prime_UTR 55.0%
GCAGACCATCGCAGTCGCTA+AGG + chr7_4:21981920-21981939 None:intergenic 60.0%
GAGGTGGGAGGGTTAAGGAG+TGG - chr7_4:21983391-21983410 Msa1145290:intron 60.0%
GCCGCGTTTGACAACAGAGG+TGG + chr7_4:21983456-21983475 None:intergenic 60.0%
TTCACAAACGCGCGAAGCGG+TGG + chr7_4:21983536-21983555 None:intergenic 60.0%
!!! GTTTCGCGGCCGCTTTTACG+TGG - chr7_4:21983562-21983581 Msa1145290:intron 60.0%
CGCTCCGCTCCACGTAAAAG+CGG + chr7_4:21983574-21983593 None:intergenic 60.0%
! CGCTTTTACGTGGAGCGGAG+CGG - chr7_4:21983572-21983591 Msa1145290:intron 60.0%
! GCTTTTACGTGGAGCGGAGC+GGG - chr7_4:21983573-21983592 Msa1145290:intron 60.0%
!! GGGTCGATAGCGGGTCAAAG+CGG - chr7_4:21983593-21983612 Msa1145290:intron 60.0%
!! GGTCGATAGCGGGTCAAAGC+GGG - chr7_4:21983594-21983613 Msa1145290:intron 60.0%
AAGCGGGCGCTATTACGGGT+CGG - chr7_4:21983610-21983629 Msa1145290:intron 60.0%
GCGGGGGCTATTACATGACG+CGG + chr7_4:21984355-21984374 None:intergenic 60.0%
ATTGACGCGGTGCGGTATGC+GGG + chr7_4:21984384-21984403 None:intergenic 60.0%
GCAGGAGTCTGTGCGATGTG+TGG - chr7_4:21985262-21985281 Msa1145290:CDS 60.0%
GCGGCCGCGTTTGACAACAG+AGG + chr7_4:21983459-21983478 None:intergenic 65.0%
TTGTCAAACGCGGCCGCTCC+AGG - chr7_4:21983462-21983481 Msa1145290:intron 65.0%
GAAGCGGTGGCGTTCTGACG+CGG + chr7_4:21983523-21983542 None:intergenic 65.0%
!!! GCGGCCGCTTTTACGTGGAG+CGG - chr7_4:21983567-21983586 Msa1145290:intron 65.0%
GTCAAAGCGGAAACGCGGCG+CGG + chr7_4:21984423-21984442 None:intergenic 65.0%
CGGTGGCGTTCTGACGCGGT+AGG + chr7_4:21983519-21983538 None:intergenic 70.0%
GGAGCGGAGCGGGTCGATAG+CGG - chr7_4:21983583-21983602 Msa1145290:intron 70.0%
GAGCGGAGCGGGTCGATAGC+GGG - chr7_4:21983584-21983603 Msa1145290:intron 70.0%
GGCCGCTCCAGGCGCTATCG+CGG - chr7_4:21983473-21983492 Msa1145290:intron 75.0%
CGCCGCGATAGCGCCTGGAG+CGG + chr7_4:21983478-21983497 None:intergenic 75.0%
CTCCAGGCGCTATCGCGGCG+CGG - chr7_4:21983478-21983497 Msa1145290:intron 75.0%
TGCGGTATGCGGGCGCGACG+CGG + chr7_4:21984374-21984393 None:intergenic 75.0%
! CTCCGCGCCGCGATAGCGCC+TGG + chr7_4:21983483-21983502 None:intergenic 80.0%
! GGCGCTATCGCGGCGCGGAG+CGG - chr7_4:21983483-21983502 Msa1145290:intron 80.0%
! GGTATGCGGGCGCGACGCGG+GGG + chr7_4:21984371-21984390 None:intergenic 80.0%
! CGGTATGCGGGCGCGACGCG+GGG + chr7_4:21984372-21984391 None:intergenic 80.0%
! GCGGTATGCGGGCGCGACGC+GGG + chr7_4:21984373-21984392 None:intergenic 80.0%
! AGCGGAAACGCGGCGCGGCG+CGG + chr7_4:21984418-21984437 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 21981714 21985690 21981714 ID=Msa1145290;Name=Msa1145290
chr7_4 mRNA 21981714 21985690 21981714 ID=Msa1145290-mRNA-1;Parent=Msa1145290;Name=Msa1145290-mRNA-1;_AED=0.01;_eAED=0.01;_QI=197|1|1|1|0.25|0.6|5|360|96
chr7_4 exon 21981714 21982138 21981714 ID=Msa1145290-mRNA-1:exon:5327;Parent=Msa1145290-mRNA-1
chr7_4 exon 21982229 21982320 21982229 ID=Msa1145290-mRNA-1:exon:5326;Parent=Msa1145290-mRNA-1
chr7_4 exon 21985248 21985365 21985248 ID=Msa1145290-mRNA-1:exon:5325;Parent=Msa1145290-mRNA-1
chr7_4 exon 21985458 21985502 21985458 ID=Msa1145290-mRNA-1:exon:5324;Parent=Msa1145290-mRNA-1
chr7_4 exon 21985523 21985690 21985523 ID=Msa1145290-mRNA-1:exon:5323;Parent=Msa1145290-mRNA-1
chr7_4 five_prime_UTR 21985523 21985690 21985523 ID=Msa1145290-mRNA-1:five_prime_utr;Parent=Msa1145290-mRNA-1
chr7_4 five_prime_UTR 21985474 21985502 21985474 ID=Msa1145290-mRNA-1:five_prime_utr;Parent=Msa1145290-mRNA-1
chr7_4 CDS 21985458 21985473 21985458 ID=Msa1145290-mRNA-1:cds;Parent=Msa1145290-mRNA-1
chr7_4 CDS 21985248 21985365 21985248 ID=Msa1145290-mRNA-1:cds;Parent=Msa1145290-mRNA-1
chr7_4 CDS 21982229 21982320 21982229 ID=Msa1145290-mRNA-1:cds;Parent=Msa1145290-mRNA-1
chr7_4 CDS 21982074 21982138 21982074 ID=Msa1145290-mRNA-1:cds;Parent=Msa1145290-mRNA-1
chr7_4 three_prime_UTR 21981714 21982073 21981714 ID=Msa1145290-mRNA-1:three_prime_utr;Parent=Msa1145290-mRNA-1
Gene Sequence

>Msa1145290

ATGGTCTGCGAAAAGTGTCAGAAGAAATTATCGAAGGTGATAGTACCAGACAAATGGAAAGAAGGTGCAAGCAATACTACTGAAGGCGGTGGCCGCAAAATCAACGAGAACAAACTCCTTTCCAAGAAGAACAGATGGACCCCTTACGGAACAACCAAGTGCATCATTTGTAAGCAGCAAGTGCACCAGGATGGCAAGTACTGTCACACGTGTGCTTACTCAAAAGGAGTCTGTGCGATGTGTGGCAAGCAAGTTCTTGACACCAAGTTTTATAAACAAAGCAACGTGTAG
Protein sequence

>Msa1145290

MVCEKCQKKLSKVIVPDKWKEGASNTTEGGGRKINENKLLSKKNRWTPYGTTKCIICKQQVHQDGKYCHTCAYSKGVCAMCGKQVLDTKFYKQSNV*