AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0184630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184630 MtrunA17_Chr1g0205991 98.039 153 3 0 1 153 1 153 2.98e-109 307
Msa0184630 MtrunA17_Chr6g0452871 54.545 110 44 1 4 107 2 111 6.81e-37 124
Msa0184630 MtrunA17_Chr1g0183501 61.667 60 22 1 6 64 7 66 2.45e-22 85.1
Msa0184630 MtrunA17_Chr1g0166511 56.364 55 24 0 3 57 2 56 4.60e-19 79.7
Msa0184630 MtrunA17_Chr1g0187421 47.541 61 31 1 5 64 20 80 7.83e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0184630 AT2G33460.1 38.194 144 81 3 1 136 1 144 5.49e-24 93.6
Msa0184630 AT4G28556.1 59.375 64 21 2 2 60 7 70 3.57e-21 85.9
Msa0184630 AT4G21745.1 50.000 92 42 3 1 91 1 89 4.33e-20 81.6
Msa0184630 AT1G04450.1 63.636 55 19 1 4 57 3 57 9.80e-20 82.0
Msa0184630 AT3G23380.1 60.345 58 22 1 1 57 1 58 2.41e-19 80.5
Msa0184630 AT3G23380.2 60.345 58 22 1 1 57 1 58 2.41e-19 80.5
Msa0184630 AT4G04900.2 61.017 59 21 2 1 57 1 59 1.61e-18 77.4
Msa0184630 AT4G04900.1 61.017 59 21 2 1 57 1 59 1.61e-18 77.4
Msa0184630 AT2G20430.1 50.725 69 31 2 2 67 7 75 4.69e-17 75.1
Msa0184630 AT1G03982.1 62.264 53 17 2 22 71 11 63 1.90e-13 64.7
Msa0184630 AT4G28556.2 72.973 37 8 1 26 60 1 37 2.52e-13 64.7
Msa0184630 AT1G61795.1 57.627 59 23 2 1 57 1 59 5.91e-13 62.4
Msa0184630 AT3G23380.3 75.000 32 8 0 26 57 1 32 3.35e-12 61.2

Find 99 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTGGTTATTCACTTATTC+TGG 0.147150 1_4:+74190206 Msa0184630:three_prime_UTR
TTGATACTCTACAACGTTAT+TGG 0.154823 1_4:+74187731 Msa0184630:five_prime_UTR
ACTTTATACGGCATTATTAT+TGG 0.165637 1_4:+74187804 Msa0184630:five_prime_UTR
ATCTTTCTTTCCCTGGTTAC+TGG 0.192124 1_4:-74190065 None:intergenic
ACCATGGCAACCACGTTTAA+AGG 0.192767 1_4:+74187840 Msa0184630:exon
ATATCTTTCAAATACTAAAA+TGG 0.194485 1_4:-74190521 None:intergenic
AAGGAGCGAGAGATGGAAAT+TGG 0.205114 1_4:+74188006 Msa0184630:CDS
TTGTCTGCAAACGAATAATT+TGG 0.235287 1_4:-74190159 None:intergenic
CCAATTGATGTCGAAAATTC+AGG 0.256562 1_4:-74188312 None:intergenic
GGAAATTTGTAATGAGTGTT+TGG 0.278209 1_4:+74190232 Msa0184630:three_prime_UTR
CTTTATACGGCATTATTATT+GGG 0.289092 1_4:+74187805 Msa0184630:five_prime_UTR
TAGGAAATTAGAGAGATATT+TGG 0.310626 1_4:+74190013 Msa0184630:three_prime_UTR
GCACTGAGGTGCTCAAAATA+TGG 0.342460 1_4:+74190408 Msa0184630:three_prime_UTR
AAAGAATACTTTGTTTCATA+TGG 0.347226 1_4:-74190041 None:intergenic
TTAAGTCAACTTCCTCTTCC+TGG 0.354143 1_4:+74189888 Msa0184630:CDS
ACCTCAGTGCATGCTCAAAA+TGG 0.354398 1_4:-74190395 None:intergenic
TTCAAGGTCAGCATATGATT+TGG 0.357796 1_4:-74190261 None:intergenic
AGAGAAGCCTAAGCAGAAAA+AGG 0.371668 1_4:+74189865 Msa0184630:CDS
GGAAAGAAAGATAATAAATA+TGG 0.375964 1_4:+74190076 Msa0184630:three_prime_UTR
GTAAAGGTAAGGAAAATCTA+TGG 0.398328 1_4:-74187487 None:intergenic
ATTTATTATCTTTCTTTCCC+TGG 0.401689 1_4:-74190072 None:intergenic
GTTGGATGGGATGGTCCCTC+AGG 0.402348 1_4:+74188060 Msa0184630:CDS
GAAATTTGTAATGAGTGTTT+GGG 0.403659 1_4:+74190233 Msa0184630:three_prime_UTR
GAAAATCTATGGATATGAAA+TGG 0.404473 1_4:-74187476 None:intergenic
TTCGACATCAATTGGTAACT+TGG 0.415150 1_4:+74188320 Msa0184630:CDS
TATCTTTCAAATACTAAAAT+GGG 0.418115 1_4:-74190520 None:intergenic
TATTGCTTCTCTCTCATTGA+AGG 0.419651 1_4:-74189960 None:intergenic
CGTGGTTATTCACTTATTCT+GGG 0.424875 1_4:+74190207 Msa0184630:three_prime_UTR
CGACACAACTTATAAGTTCA+AGG 0.426916 1_4:-74190277 None:intergenic
AAGTACATTACTCAGATGTT+TGG 0.428512 1_4:+74187879 Msa0184630:CDS
TGAAAGTAGGAGGTGGAAGA+AGG 0.428583 1_4:-74187384 None:intergenic
ATCTGCTACACCAGAGCAAT+TGG 0.434357 1_4:-74189983 None:intergenic
ATATAAACACCATACCCAAC+TGG 0.441516 1_4:-74188091 None:intergenic
CTATGGCTGTCACTTCATTA+TGG 0.445205 1_4:+74188364 Msa0184630:CDS
TCCTTTAAACGTGGTTGCCA+TGG 0.449019 1_4:-74187841 None:intergenic
CTCAGGGAATGGACCCAGTT+GGG 0.465751 1_4:+74188077 Msa0184630:CDS
GAAAGTAGGAGGTGGAAGAA+GGG 0.466205 1_4:-74187383 None:intergenic
GAACAAATGAGAAAATCATC+TGG 0.466403 1_4:-74190544 None:intergenic
AAGAAGGGTTTGACTCTAGA+AGG 0.469038 1_4:-74187368 None:intergenic
TGGGATGGTCCCTCAGGGAA+TGG 0.472703 1_4:+74188066 Msa0184630:CDS
AGGAGGTTGAGAAGAGAAGG+TGG 0.473362 1_4:-74187348 None:intergenic
AAAGGCATCTCATCTGCTGC+AGG 0.474060 1_4:+74189827 Msa0184630:CDS
TCTTTCTTTCCCTGGTTACT+GGG 0.477566 1_4:-74190064 None:intergenic
AGTATTCTTTCCCAGTAACC+AGG 0.482131 1_4:+74190054 Msa0184630:three_prime_UTR
AATGGAAGAAAATATGTAAT+GGG 0.485099 1_4:-74187448 None:intergenic
TTAGGCTTCTCTGCAATATG+AGG 0.487591 1_4:-74189854 None:intergenic
AGGTATTTGCAGCGTTCAGA+TGG 0.493670 1_4:+74190434 Msa0184630:three_prime_UTR
AGGTGCTCAAAATATGGAAA+AGG 0.503103 1_4:+74190414 Msa0184630:three_prime_UTR
AGGAGCGAGAGATGGAAATT+GGG 0.509528 1_4:+74188007 Msa0184630:CDS
AATTAGAGACTCTGATCCTA+TGG 0.510482 1_4:+74188347 Msa0184630:CDS
GTTATTCACTTATTCTGGGA+AGG 0.514220 1_4:+74190211 Msa0184630:three_prime_UTR
AGAAGCAATACCAATTGCTC+TGG 0.524560 1_4:+74189973 Msa0184630:CDS
TCTATGGATATGAAATGGAA+TGG 0.526863 1_4:-74187471 None:intergenic
GTGGCTCATGTTGGATGGGA+TGG 0.528972 1_4:+74188051 Msa0184630:CDS
CATAATGAAGTGACAGCCAT+AGG 0.529806 1_4:-74188363 None:intergenic
GAATGGAAGAAAATATGTAA+TGG 0.537302 1_4:-74187449 None:intergenic
AGAAGGAGGTTGAGAAGAGA+AGG 0.537768 1_4:-74187351 None:intergenic
AGTTGTGAAGGAGCGAGAGA+TGG 0.540742 1_4:+74187999 Msa0184630:intron
GGAGGTTGAGAAGAGAAGGT+GGG 0.542237 1_4:-74187347 None:intergenic
TAATTTACTAACTAGAAGCC+AGG 0.542740 1_4:-74190112 None:intergenic
ATTCATGTTGAAACATGTAA+AGG 0.543248 1_4:-74187503 None:intergenic
TGTTGAAACATGTAAAGGTA+AGG 0.543333 1_4:-74187498 None:intergenic
GGATATGAAATGGAATGGAA+TGG 0.544274 1_4:-74187466 None:intergenic
ATCAAGAGCATCAAAATCAA+AGG 0.551691 1_4:+74189934 Msa0184630:CDS
ATGAAAGTGAAAGTAGGAGG+TGG 0.552421 1_4:-74187391 None:intergenic
ACATGAATTTCAAAACCTGC+AGG 0.552558 1_4:+74187518 Msa0184630:five_prime_UTR
AGGCATCTCATCTGCTGCAG+GGG 0.558500 1_4:+74189829 Msa0184630:CDS
AACATGTGGCTCATGTTGGA+TGG 0.559701 1_4:+74188046 Msa0184630:CDS
AAATTATAATCTAGTCCAGT+GGG 0.561409 1_4:+74190129 Msa0184630:three_prime_UTR
AGATGATGGTGACCAGGAAG+AGG 0.563076 1_4:-74189900 None:intergenic
GAAGATTTAAAGGAATGGCA+TGG 0.568361 1_4:+74190308 Msa0184630:three_prime_UTR
GGTGTAGCAGATTCAAGACT+AGG 0.573682 1_4:+74189994 Msa0184630:exon
GTTAAACATGTGGCTCATGT+TGG 0.581035 1_4:+74188042 Msa0184630:CDS
CCTCAGGGAATGGACCCAGT+TGG 0.581964 1_4:+74188076 Msa0184630:CDS
ACGTTGTAGAGTATCAACTA+AGG 0.582432 1_4:-74187725 None:intergenic
ACTTCATTATGGTCCTCCAT+AGG 0.586915 1_4:+74188375 Msa0184630:CDS
CCACATGTTTAACATCCGTC+GGG 0.587906 1_4:-74188032 None:intergenic
AAGGCATCTCATCTGCTGCA+GGG 0.590720 1_4:+74189828 Msa0184630:CDS
GCCACATGTTTAACATCCGT+CGG 0.595209 1_4:-74188033 None:intergenic
TATAAACACCATACCCAACT+GGG 0.596139 1_4:-74188090 None:intergenic
CTTACAAATTCCTTTAAACG+TGG 0.598013 1_4:-74187850 None:intergenic
ATGCACAGATAAACTCTACA+AGG 0.609804 1_4:-74190458 None:intergenic
TTGGATGGGATGGTCCCTCA+GGG 0.611512 1_4:+74188061 Msa0184630:CDS
ACATGTGGCTCATGTTGGAT+GGG 0.613430 1_4:+74188047 Msa0184630:CDS
TGATGAAGATGATGGTGACC+AGG 0.620233 1_4:-74189906 None:intergenic
GATCGTCTTGATGAAGATGA+TGG 0.625529 1_4:-74189914 None:intergenic
TAAATTATAATCTAGTCCAG+TGG 0.627743 1_4:+74190128 Msa0184630:three_prime_UTR
ATAGTTGTATGTCCGTCCTG+TGG 0.632873 1_4:+74190340 Msa0184630:three_prime_UTR
TGGGATTGTGATCTTCACCA+TGG 0.633325 1_4:+74187824 Msa0184630:exon
AAGGGTTTGACTCTAGAAGG+AGG 0.633887 1_4:-74187365 None:intergenic
CAATCATCAAACATTTACAA+AGG 0.636264 1_4:+74189809 Msa0184630:CDS
GTATTCTTTCCCAGTAACCA+GGG 0.643305 1_4:+74190055 Msa0184630:three_prime_UTR
GAAGCAAAGCATACCTATGG+AGG 0.644836 1_4:-74188388 None:intergenic
GGTATGAAAGTGAAAGTAGG+AGG 0.655406 1_4:-74187394 None:intergenic
CCCGACGGATGTTAAACATG+TGG 0.657287 1_4:+74188032 Msa0184630:CDS
GATGGAAATTGGGTACCCGA+CGG 0.676519 1_4:+74188017 Msa0184630:CDS
CAACTGGGTCCATTCCCTGA+GGG 0.690377 1_4:-74188075 None:intergenic
GATTTAAAGGAATGGCATGG+TGG 0.699903 1_4:+74190311 Msa0184630:three_prime_UTR
CCAACTGGGTCCATTCCCTG+AGG 0.728969 1_4:-74188076 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGATTATTGAAAATTATT+GGG + chr1_4:74189343-74189362 Msa0184630:intron 10.0%
!! ATGTAAAATGATAATTTCTA+CGG - chr1_4:74187607-74187626 None:intergenic 15.0%
!! AAAATAATATACAAAATGTC+TGG - chr1_4:74188244-74188263 None:intergenic 15.0%
!! TATTAAAAATTTGATAGAGA+TGG + chr1_4:74188786-74188805 Msa0184630:intron 15.0%
!!! CATATTTTAAAATTTACTTC+AGG + chr1_4:74189316-74189335 Msa0184630:intron 15.0%
!!! ATAATTTTCAATAATCAATG+AGG - chr1_4:74189342-74189361 None:intergenic 15.0%
!!! CATTGATTATTGAAAATTAT+TGG + chr1_4:74189342-74189361 Msa0184630:intron 15.0%
!! TATCTTTCAAATACTAAAAT+GGG - chr1_4:74190523-74190542 None:intergenic 15.0%
!! ATATCTTTCAAATACTAAAA+TGG - chr1_4:74190524-74190543 None:intergenic 15.0%
!! AATGGAAGAAAATATGTAAT+GGG - chr1_4:74187451-74187470 None:intergenic 20.0%
!!! TTTTGTGTCTTTACTTTATA+CGG + chr1_4:74187792-74187811 Msa0184630:five_prime_UTR 20.0%
!! ATCACATTAAACACATTAAT+TGG - chr1_4:74187929-74187948 None:intergenic 20.0%
!! TTTGGACTAAAATATGAAAT+GGG + chr1_4:74187967-74187986 Msa0184630:intron 20.0%
!!! CAATTTCTCTTCTTTTTTTA+AGG - chr1_4:74188737-74188756 None:intergenic 20.0%
!! AAAACAATCTATATATTCCA+AGG + chr1_4:74189048-74189067 Msa0184630:intron 20.0%
!! AAACAATCTATATATTCCAA+GGG + chr1_4:74189049-74189068 Msa0184630:intron 20.0%
!!! AAAGACAGATAAATTTTTGT+TGG - chr1_4:74189252-74189271 None:intergenic 20.0%
!! ATTATTGAAAATTATTGGGT+TGG + chr1_4:74189347-74189366 Msa0184630:intron 20.0%
!!! TAAACACAAATTTTAGCATA+TGG - chr1_4:74189426-74189445 None:intergenic 20.0%
!!! ATATTTTGAAACCTAAATTC+TGG - chr1_4:74189455-74189474 None:intergenic 20.0%
!! AGAATTTAATCAGTATTGTA+TGG - chr1_4:74189554-74189573 None:intergenic 20.0%
!! GAAATAAATAAATTTGTCCA+TGG - chr1_4:74189609-74189628 None:intergenic 20.0%
!!! ATCAGATCTTATTTGTTATT+TGG + chr1_4:74189732-74189751 Msa0184630:intron 20.0%
!!! AAAGAATACTTTGTTTCATA+TGG - chr1_4:74190044-74190063 None:intergenic 20.0%
!! GGAAAGAAAGATAATAAATA+TGG + chr1_4:74190076-74190095 Msa0184630:three_prime_UTR 20.0%
!!! ATTTTGAAGATTTAAAGGAA+TGG + chr1_4:74190303-74190322 Msa0184630:three_prime_UTR 20.0%
!!! AAGTTGAGATTTTGATTAGT+GGG - chr1_4:74187421-74187440 None:intergenic 25.0%
!!! AAAGTTGAGATTTTGATTAG+TGG - chr1_4:74187422-74187441 None:intergenic 25.0%
! GAATGGAAGAAAATATGTAA+TGG - chr1_4:74187452-74187471 None:intergenic 25.0%
! GAAAATCTATGGATATGAAA+TGG - chr1_4:74187479-74187498 None:intergenic 25.0%
! ATTCATGTTGAAACATGTAA+AGG - chr1_4:74187506-74187525 None:intergenic 25.0%
! ACTTTATACGGCATTATTAT+TGG + chr1_4:74187804-74187823 Msa0184630:five_prime_UTR 25.0%
! CTTTATACGGCATTATTATT+GGG + chr1_4:74187805-74187824 Msa0184630:five_prime_UTR 25.0%
!!! TTCTGTGTTTTGTTATTGTT+TGG + chr1_4:74187949-74187968 Msa0184630:intron 25.0%
! GTTTGGACTAAAATATGAAA+TGG + chr1_4:74187966-74187985 Msa0184630:intron 25.0%
!!! CTTTTTTAAAATCATCCATC+TGG - chr1_4:74188290-74188309 None:intergenic 25.0%
!! TTGATTATTGTTGTGTGTTT+TGG + chr1_4:74188466-74188485 Msa0184630:intron 25.0%
!!! TAACACTGTTTTTTTAAGTG+TGG + chr1_4:74188626-74188645 Msa0184630:intron 25.0%
!! TTGTTAAGTACATGTTTTGT+TGG + chr1_4:74188664-74188683 Msa0184630:intron 25.0%
! TATTGATAAAGCTTATCACA+TGG - chr1_4:74188702-74188721 None:intergenic 25.0%
! ATCATGACTACATAAATACA+AGG - chr1_4:74188887-74188906 None:intergenic 25.0%
! TGTATTTATGTAGTCATGAT+TGG + chr1_4:74188887-74188906 Msa0184630:intron 25.0%
! TAAGAAGTTAACATGAAACA+AGG + chr1_4:74189141-74189160 Msa0184630:intron 25.0%
!! ACAGATAAATTTTTGTTGGT+GGG - chr1_4:74189248-74189267 None:intergenic 25.0%
! GTTTAATAACTCCAGAATTT+AGG + chr1_4:74189441-74189460 Msa0184630:intron 25.0%
!! AAATATACTGTTTAGCTTCA+TGG + chr1_4:74189469-74189488 Msa0184630:intron 25.0%
! GAATAGGTATCATTAAGAAT+AGG - chr1_4:74189771-74189790 None:intergenic 25.0%
! CAATCATCAAACATTTACAA+AGG + chr1_4:74189809-74189828 Msa0184630:CDS 25.0%
! TAGGAAATTAGAGAGATATT+TGG + chr1_4:74190013-74190032 Msa0184630:three_prime_UTR 25.0%
! ATTTATTATCTTTCTTTCCC+TGG - chr1_4:74190075-74190094 None:intergenic 25.0%
! TAAATTATAATCTAGTCCAG+TGG + chr1_4:74190128-74190147 Msa0184630:three_prime_UTR 25.0%
! AAATTATAATCTAGTCCAGT+GGG + chr1_4:74190129-74190148 Msa0184630:three_prime_UTR 25.0%
! GAAATTTGTAATGAGTGTTT+GGG + chr1_4:74190233-74190252 Msa0184630:three_prime_UTR 25.0%
!!! CGTCAATTTTGAAGATTTAA+AGG + chr1_4:74190298-74190317 Msa0184630:three_prime_UTR 25.0%
!!! AGTTGAGATTTTGATTAGTG+GGG - chr1_4:74187420-74187439 None:intergenic 30.0%
TCTATGGATATGAAATGGAA+TGG - chr1_4:74187474-74187493 None:intergenic 30.0%
GTAAAGGTAAGGAAAATCTA+TGG - chr1_4:74187490-74187509 None:intergenic 30.0%
TGTTGAAACATGTAAAGGTA+AGG - chr1_4:74187501-74187520 None:intergenic 30.0%
TTGATACTCTACAACGTTAT+TGG + chr1_4:74187731-74187750 Msa0184630:five_prime_UTR 30.0%
CTTACAAATTCCTTTAAACG+TGG - chr1_4:74187853-74187872 None:intergenic 30.0%
AAGTACATTACTCAGATGTT+TGG + chr1_4:74187879-74187898 Msa0184630:CDS 30.0%
GGTATGGTGTTTATATAGAT+TGG + chr1_4:74188098-74188117 Msa0184630:intron 30.0%
! TACAAAATGTCTGGAATCAT+CGG - chr1_4:74188235-74188254 None:intergenic 30.0%
ACAAATGTATTGTCCTTTAC+CGG + chr1_4:74188415-74188434 Msa0184630:intron 30.0%
!!! CATTTTTTCGACTAATTGAC+CGG - chr1_4:74188437-74188456 None:intergenic 30.0%
! ATGTTCTTGATAAAAGTGTG+AGG + chr1_4:74188491-74188510 Msa0184630:intron 30.0%
AACCATAAGATTCTCTTTGT+TGG - chr1_4:74188590-74188609 None:intergenic 30.0%
!! TACATGTTTTGTTGGTACTT+TGG + chr1_4:74188672-74188691 Msa0184630:intron 30.0%
TACATAAATACAAGGCTAGT+TGG - chr1_4:74188879-74188898 None:intergenic 30.0%
TTTATGTAGTCATGATTGGA+TGG + chr1_4:74188891-74188910 Msa0184630:intron 30.0%
ATGATTGGATGGATGTTTAA+AGG + chr1_4:74188902-74188921 Msa0184630:intron 30.0%
AGATACTTTGTCTTTCTTGA+AGG + chr1_4:74188992-74189011 Msa0184630:intron 30.0%
GATACTTTGTCTTTCTTGAA+GGG + chr1_4:74188993-74189012 Msa0184630:intron 30.0%
ATACTTTGTCTTTCTTGAAG+GGG + chr1_4:74188994-74189013 Msa0184630:intron 30.0%
AATCTATATATTCCAAGGGT+GGG + chr1_4:74189053-74189072 Msa0184630:intron 30.0%
TTAGTCAGATACTCCAAATT+TGG - chr1_4:74189086-74189105 None:intergenic 30.0%
TTCTGTTTGAAAGATAACAG+TGG + chr1_4:74189172-74189191 Msa0184630:intron 30.0%
! GACAGATAAATTTTTGTTGG+TGG - chr1_4:74189249-74189268 None:intergenic 30.0%
TGTAATCACAATGCAATTGT+TGG - chr1_4:74189631-74189650 None:intergenic 30.0%
TTACATTTCCTTCCATTTCT+AGG + chr1_4:74189646-74189665 Msa0184630:intron 30.0%
GTATCATTAAGAATAGGAAC+TGG - chr1_4:74189765-74189784 None:intergenic 30.0%
!! ATCAAGAGCATCAAAATCAA+AGG + chr1_4:74189934-74189953 Msa0184630:CDS 30.0%
TAATTTACTAACTAGAAGCC+AGG - chr1_4:74190115-74190134 None:intergenic 30.0%
TTGTCTGCAAACGAATAATT+TGG - chr1_4:74190162-74190181 None:intergenic 30.0%
CAATGAAAAAAACACTAACG+TGG + chr1_4:74190189-74190208 Msa0184630:three_prime_UTR 30.0%
GGAAATTTGTAATGAGTGTT+TGG + chr1_4:74190232-74190251 Msa0184630:three_prime_UTR 30.0%
GAACAAATGAGAAAATCATC+TGG - chr1_4:74190547-74190566 None:intergenic 30.0%
!!! TTGAGATTTTGATTAGTGGG+GGG - chr1_4:74187418-74187437 None:intergenic 35.0%
!!! GTTGAGATTTTGATTAGTGG+GGG - chr1_4:74187419-74187438 None:intergenic 35.0%
GGATATGAAATGGAATGGAA+TGG - chr1_4:74187469-74187488 None:intergenic 35.0%
ACATGAATTTCAAAACCTGC+AGG + chr1_4:74187518-74187537 Msa0184630:five_prime_UTR 35.0%
AGATGCTTATCTTTACTCAC+AGG + chr1_4:74187660-74187679 Msa0184630:intron 35.0%
ACTCACAGGAAAACAAAAAG+AGG + chr1_4:74187674-74187693 Msa0184630:intron 35.0%
! ACGTTGTAGAGTATCAACTA+AGG - chr1_4:74187728-74187747 None:intergenic 35.0%
TATAAACACCATACCCAACT+GGG - chr1_4:74188093-74188112 None:intergenic 35.0%
ATATAAACACCATACCCAAC+TGG - chr1_4:74188094-74188113 None:intergenic 35.0%
! ACAAAATGTCTGGAATCATC+GGG - chr1_4:74188234-74188253 None:intergenic 35.0%
! AAATCATCCATCTGGAATCA+CGG - chr1_4:74188282-74188301 None:intergenic 35.0%
CCAATTGATGTCGAAAATTC+AGG - chr1_4:74188315-74188334 None:intergenic 35.0%
! CCTGAATTTTCGACATCAAT+TGG + chr1_4:74188312-74188331 Msa0184630:CDS 35.0%
! TTCGACATCAATTGGTAACT+TGG + chr1_4:74188320-74188339 Msa0184630:CDS 35.0%
AATTAGAGACTCTGATCCTA+TGG + chr1_4:74188347-74188366 Msa0184630:CDS 35.0%
AACAGCCAAACTCTTTCTAA+GGG - chr1_4:74188546-74188565 None:intergenic 35.0%
CTCCAACAAAGAGAATCTTA+TGG + chr1_4:74188585-74188604 Msa0184630:intron 35.0%
CATGTACTTAACAAGCATTC+TGG - chr1_4:74188658-74188677 None:intergenic 35.0%
TGTCACGTTCTAGTATGAAT+TGG + chr1_4:74188831-74188850 Msa0184630:intron 35.0%
! TAAATACAAGGCTAGTTGGA+AGG - chr1_4:74188875-74188894 None:intergenic 35.0%
!! GCAAAGCTGATTGTTTTTCT+TGG - chr1_4:74188943-74188962 None:intergenic 35.0%
ACAGTCATGGCTAATTCTTA+AGG - chr1_4:74188967-74188986 None:intergenic 35.0%
CAATCTATATATTCCAAGGG+TGG + chr1_4:74189052-74189071 Msa0184630:intron 35.0%
! TGGTGATTATCCTTTTCTGA+AGG + chr1_4:74189489-74189508 Msa0184630:intron 35.0%
ATTCTCATCCTAGAAATGGA+AGG - chr1_4:74189657-74189676 None:intergenic 35.0%
CATGATTCTCATCCTAGAAA+TGG - chr1_4:74189661-74189680 None:intergenic 35.0%
ATTAAGAATAGGAACTGGCA+TGG - chr1_4:74189760-74189779 None:intergenic 35.0%
! GATTTTCTGTGCACAAGAAT+AGG - chr1_4:74189787-74189806 None:intergenic 35.0%
! GACTTAACCTTTTTCTGCTT+AGG - chr1_4:74189875-74189894 None:intergenic 35.0%
TATTGCTTCTCTCTCATTGA+AGG - chr1_4:74189963-74189982 None:intergenic 35.0%
! TATGGCAGTGAAAATTTTCC+TGG + chr1_4:74190094-74190113 Msa0184630:three_prime_UTR 35.0%
!!! TAATTTGGTTTTACTCCCAC+TGG - chr1_4:74190147-74190166 None:intergenic 35.0%
ACGTGGTTATTCACTTATTC+TGG + chr1_4:74190206-74190225 Msa0184630:three_prime_UTR 35.0%
CGTGGTTATTCACTTATTCT+GGG + chr1_4:74190207-74190226 Msa0184630:three_prime_UTR 35.0%
! GTTATTCACTTATTCTGGGA+AGG + chr1_4:74190211-74190230 Msa0184630:three_prime_UTR 35.0%
TTCAAGGTCAGCATATGATT+TGG - chr1_4:74190264-74190283 None:intergenic 35.0%
CGACACAACTTATAAGTTCA+AGG - chr1_4:74190280-74190299 None:intergenic 35.0%
GAAGATTTAAAGGAATGGCA+TGG + chr1_4:74190308-74190327 Msa0184630:three_prime_UTR 35.0%
AACAAGAAAAAACATGCCAC+AGG - chr1_4:74190359-74190378 None:intergenic 35.0%
AGGTGCTCAAAATATGGAAA+AGG + chr1_4:74190414-74190433 Msa0184630:three_prime_UTR 35.0%
ATGCACAGATAAACTCTACA+AGG - chr1_4:74190461-74190480 None:intergenic 35.0%
AAGAAGGGTTTGACTCTAGA+AGG - chr1_4:74187371-74187390 None:intergenic 40.0%
ATGAAAGTGAAAGTAGGAGG+TGG - chr1_4:74187394-74187413 None:intergenic 40.0%
GGTATGAAAGTGAAAGTAGG+AGG - chr1_4:74187397-74187416 None:intergenic 40.0%
GAGTATAGTATAGTACCTGC+AGG - chr1_4:74187536-74187555 None:intergenic 40.0%
GTTAAACATGTGGCTCATGT+TGG + chr1_4:74188042-74188061 Msa0184630:CDS 40.0%
CATAATGAAGTGACAGCCAT+AGG - chr1_4:74188366-74188385 None:intergenic 40.0%
! CTATGGCTGTCACTTCATTA+TGG + chr1_4:74188364-74188383 Msa0184630:CDS 40.0%
ACTTCATTATGGTCCTCCAT+AGG + chr1_4:74188375-74188394 Msa0184630:CDS 40.0%
CAAGAAGCAAAGCATACCTA+TGG - chr1_4:74188394-74188413 None:intergenic 40.0%
TTCGACTAATTGACCGGTAA+AGG - chr1_4:74188431-74188450 None:intergenic 40.0%
TTGCTCCCTTAGAAAGAGTT+TGG + chr1_4:74188538-74188557 Msa0184630:intron 40.0%
GAACAGCCAAACTCTTTCTA+AGG - chr1_4:74188547-74188566 None:intergenic 40.0%
ACTTAACAAGCATTCTGGTG+TGG - chr1_4:74188653-74188672 None:intergenic 40.0%
ACAGCAATGTCTACTAGTGA+TGG - chr1_4:74188814-74188833 None:intergenic 40.0%
AAGTATCTCAGTCACAGTCA+TGG - chr1_4:74188980-74188999 None:intergenic 40.0%
AAAGATAACAGTGGCACTCA+GGG + chr1_4:74189181-74189200 Msa0184630:intron 40.0%
TTAGGCTTCTCTGCAATATG+AGG - chr1_4:74189857-74189876 None:intergenic 40.0%
AGAGAAGCCTAAGCAGAAAA+AGG + chr1_4:74189865-74189884 Msa0184630:CDS 40.0%
TTAAGTCAACTTCCTCTTCC+TGG + chr1_4:74189888-74189907 Msa0184630:CDS 40.0%
GATCGTCTTGATGAAGATGA+TGG - chr1_4:74189917-74189936 None:intergenic 40.0%
AGAAGCAATACCAATTGCTC+TGG + chr1_4:74189973-74189992 Msa0184630:CDS 40.0%
AGTATTCTTTCCCAGTAACC+AGG + chr1_4:74190054-74190073 Msa0184630:three_prime_UTR 40.0%
GTATTCTTTCCCAGTAACCA+GGG + chr1_4:74190055-74190074 Msa0184630:three_prime_UTR 40.0%
TCTTTCTTTCCCTGGTTACT+GGG - chr1_4:74190067-74190086 None:intergenic 40.0%
ATCTTTCTTTCCCTGGTTAC+TGG - chr1_4:74190068-74190087 None:intergenic 40.0%
GATTTAAAGGAATGGCATGG+TGG + chr1_4:74190311-74190330 Msa0184630:three_prime_UTR 40.0%
AGAAAAAACATGCCACAGGA+CGG - chr1_4:74190355-74190374 None:intergenic 40.0%
AGAAGGAGGTTGAGAAGAGA+AGG - chr1_4:74187354-74187373 None:intergenic 45.0%
! AAGGGTTTGACTCTAGAAGG+AGG - chr1_4:74187368-74187387 None:intergenic 45.0%
GAAAGTAGGAGGTGGAAGAA+GGG - chr1_4:74187386-74187405 None:intergenic 45.0%
TGAAAGTAGGAGGTGGAAGA+AGG - chr1_4:74187387-74187406 None:intergenic 45.0%
! GGGGGTATGAAAGTGAAAGT+AGG - chr1_4:74187400-74187419 None:intergenic 45.0%
TGGGATTGTGATCTTCACCA+TGG + chr1_4:74187824-74187843 Msa0184630:exon 45.0%
ACCATGGCAACCACGTTTAA+AGG + chr1_4:74187840-74187859 Msa0184630:exon 45.0%
TCCTTTAAACGTGGTTGCCA+TGG - chr1_4:74187844-74187863 None:intergenic 45.0%
AAGGAGCGAGAGATGGAAAT+TGG + chr1_4:74188006-74188025 Msa0184630:CDS 45.0%
AGGAGCGAGAGATGGAAATT+GGG + chr1_4:74188007-74188026 Msa0184630:CDS 45.0%
CCACATGTTTAACATCCGTC+GGG - chr1_4:74188035-74188054 None:intergenic 45.0%
GCCACATGTTTAACATCCGT+CGG - chr1_4:74188036-74188055 None:intergenic 45.0%
AACATGTGGCTCATGTTGGA+TGG + chr1_4:74188046-74188065 Msa0184630:CDS 45.0%
ACATGTGGCTCATGTTGGAT+GGG + chr1_4:74188047-74188066 Msa0184630:CDS 45.0%
! TTTTATGCTGCGCGCATGTT+TGG + chr1_4:74188157-74188176 Msa0184630:intron 45.0%
GTTTGGATTCACGGTGAGTT+TGG + chr1_4:74188174-74188193 Msa0184630:intron 45.0%
! TTTTCTGCCGTGATTCCAGA+TGG + chr1_4:74188272-74188291 Msa0184630:intron 45.0%
GAAGCAAAGCATACCTATGG+AGG - chr1_4:74188391-74188410 None:intergenic 45.0%
! ATACAAGGCTAGTTGGAAGG+CGG - chr1_4:74188872-74188891 None:intergenic 45.0%
GAAAGATAACAGTGGCACTC+AGG + chr1_4:74189180-74189199 Msa0184630:intron 45.0%
! TTTAGTGGCCTTACACCCTT+AGG - chr1_4:74189211-74189230 None:intergenic 45.0%
TGCTCCGTATGCAGACAAAA+AGG + chr1_4:74189290-74189309 Msa0184630:intron 45.0%
! GCGACCTTTTTGTCTGCATA+CGG - chr1_4:74189297-74189316 None:intergenic 45.0%
!! GAAAATTATTGGGTTGGCCC+TGG + chr1_4:74189353-74189372 Msa0184630:intron 45.0%
ATGCGTAGAGCCTTCAGAAA+AGG - chr1_4:74189502-74189521 None:intergenic 45.0%
AAATGCATTGCGTCGTACCA+TGG + chr1_4:74189589-74189608 Msa0184630:intron 45.0%
! TGATGAAGATGATGGTGACC+AGG - chr1_4:74189909-74189928 None:intergenic 45.0%
ATCTGCTACACCAGAGCAAT+TGG - chr1_4:74189986-74190005 None:intergenic 45.0%
GGTGTAGCAGATTCAAGACT+AGG + chr1_4:74189994-74190013 Msa0184630:exon 45.0%
ATAGTTGTATGTCCGTCCTG+TGG + chr1_4:74190340-74190359 Msa0184630:three_prime_UTR 45.0%
! TCCATTTTGAGCATGCACTG+AGG + chr1_4:74190394-74190413 Msa0184630:three_prime_UTR 45.0%
ACCTCAGTGCATGCTCAAAA+TGG - chr1_4:74190398-74190417 None:intergenic 45.0%
! GCACTGAGGTGCTCAAAATA+TGG + chr1_4:74190408-74190427 Msa0184630:three_prime_UTR 45.0%
AGGTATTTGCAGCGTTCAGA+TGG + chr1_4:74190434-74190453 Msa0184630:three_prime_UTR 45.0%
GGAGGTTGAGAAGAGAAGGT+GGG - chr1_4:74187350-74187369 None:intergenic 50.0%
AGGAGGTTGAGAAGAGAAGG+TGG - chr1_4:74187351-74187370 None:intergenic 50.0%
! GGGTCTTTTCCCAGTTGTGA+AGG + chr1_4:74187987-74188006 Msa0184630:intron 50.0%
TCTCTCGCTCCTTCACAACT+GGG - chr1_4:74187999-74188018 None:intergenic 50.0%
ATCTCTCGCTCCTTCACAAC+TGG - chr1_4:74188000-74188019 None:intergenic 50.0%
AGTTGTGAAGGAGCGAGAGA+TGG + chr1_4:74187999-74188018 Msa0184630:intron 50.0%
GATGGAAATTGGGTACCCGA+CGG + chr1_4:74188017-74188036 Msa0184630:CDS 50.0%
CCCGACGGATGTTAAACATG+TGG + chr1_4:74188032-74188051 Msa0184630:CDS 50.0%
TGCGCGCATGTTTGGATTCA+CGG + chr1_4:74188165-74188184 Msa0184630:intron 50.0%
GGTGGGTCTAGTGCCAAATT+TGG + chr1_4:74189070-74189089 Msa0184630:intron 50.0%
! GCCACTAAAGCCCTAGCTTT+AGG + chr1_4:74189222-74189241 Msa0184630:intron 50.0%
! GCCTAAAGCTAGGGCTTTAG+TGG - chr1_4:74189226-74189245 None:intergenic 50.0%
! TGTTGGTGGGCCTAAAGCTA+GGG - chr1_4:74189235-74189254 None:intergenic 50.0%
! TTGTTGGTGGGCCTAAAGCT+AGG - chr1_4:74189236-74189255 None:intergenic 50.0%
!! AATTATTGGGTTGGCCCTGG+CGG + chr1_4:74189356-74189375 Msa0184630:intron 50.0%
CTGCATGTTACCTTCGACTG+TGG + chr1_4:74189525-74189544 Msa0184630:intron 50.0%
TGTATGGTGACCACAGTCGA+AGG - chr1_4:74189538-74189557 None:intergenic 50.0%
!! AAAGGCATCTCATCTGCTGC+AGG + chr1_4:74189827-74189846 Msa0184630:CDS 50.0%
! AAGGCATCTCATCTGCTGCA+GGG + chr1_4:74189828-74189847 Msa0184630:CDS 50.0%
! AGATGATGGTGACCAGGAAG+AGG - chr1_4:74189903-74189922 None:intergenic 50.0%
GTGGCTCATGTTGGATGGGA+TGG + chr1_4:74188051-74188070 Msa0184630:CDS 55.0%
TTGGATGGGATGGTCCCTCA+GGG + chr1_4:74188061-74188080 Msa0184630:CDS 55.0%
CAACTGGGTCCATTCCCTGA+GGG - chr1_4:74188078-74188097 None:intergenic 55.0%
CTCAGGGAATGGACCCAGTT+GGG + chr1_4:74188077-74188096 Msa0184630:CDS 55.0%
GGAATGGACCCAGTTGGGTA+TGG + chr1_4:74188082-74188101 Msa0184630:intron 55.0%
TTTGGCACTAGACCCACCCT+TGG - chr1_4:74189068-74189087 None:intergenic 55.0%
ATAACAGTGGCACTCAGGGC+CGG + chr1_4:74189185-74189204 Msa0184630:intron 55.0%
AGGCATCTCATCTGCTGCAG+GGG + chr1_4:74189829-74189848 Msa0184630:CDS 55.0%
GTTGGATGGGATGGTCCCTC+AGG + chr1_4:74188060-74188079 Msa0184630:CDS 60.0%
TGGGATGGTCCCTCAGGGAA+TGG + chr1_4:74188066-74188085 Msa0184630:CDS 60.0%
CCAACTGGGTCCATTCCCTG+AGG - chr1_4:74188079-74188098 None:intergenic 60.0%
CCTCAGGGAATGGACCCAGT+TGG + chr1_4:74188076-74188095 Msa0184630:CDS 60.0%
! GTGGCCTTACACCCTTAGGC+CGG - chr1_4:74189207-74189226 None:intergenic 60.0%
!! ATCCATGCAGAGTGCCGCCA+GGG - chr1_4:74189373-74189392 None:intergenic 60.0%
TGCATCCATGCACAGTGCCG+CGG - chr1_4:74189397-74189416 None:intergenic 60.0%
! AGGGCCGGCCTAAGGGTGTA+AGG + chr1_4:74189200-74189219 Msa0184630:intron 65.0%
!! CATCCATGCAGAGTGCCGCC+AGG - chr1_4:74189374-74189393 None:intergenic 65.0%
GGCACTCTGCATGGATGCCG+CGG + chr1_4:74189377-74189396 Msa0184630:intron 65.0%
TGGCACTCAGGGCCGGCCTA+AGG + chr1_4:74189192-74189211 Msa0184630:intron 70.0%
GGCACTCAGGGCCGGCCTAA+GGG + chr1_4:74189193-74189212 Msa0184630:intron 70.0%
GGATGCCGCGGCACTGTGCA+TGG + chr1_4:74189389-74189408 Msa0184630:intron 70.0%
GGCCCTGGCGGCACTCTGCA+TGG + chr1_4:74189368-74189387 Msa0184630:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 74187342 74190566 74187342 ID=Msa0184630;Name=Msa0184630
chr1_4 mRNA 74187342 74190566 74187342 ID=Msa0184630-mRNA-1;Parent=Msa0184630;Name=Msa0184630-mRNA-1;_AED=0.07;_eAED=0.07;_QI=359|1|1|1|0.75|0.6|5|566|153
chr1_4 exon 74187342 74187539 74187342 ID=Msa0184630-mRNA-1:exon:25274;Parent=Msa0184630-mRNA-1
chr1_4 exon 74187682 74187900 74187682 ID=Msa0184630-mRNA-1:exon:25275;Parent=Msa0184630-mRNA-1
chr1_4 exon 74188001 74188098 74188001 ID=Msa0184630-mRNA-1:exon:25276;Parent=Msa0184630-mRNA-1
chr1_4 exon 74188291 74188396 74188291 ID=Msa0184630-mRNA-1:exon:25277;Parent=Msa0184630-mRNA-1
chr1_4 exon 74189801 74190566 74189801 ID=Msa0184630-mRNA-1:exon:25278;Parent=Msa0184630-mRNA-1
chr1_4 five_prime_UTR 74187342 74187539 74187342 ID=Msa0184630-mRNA-1:five_prime_utr;Parent=Msa0184630-mRNA-1
chr1_4 five_prime_UTR 74187682 74187842 74187682 ID=Msa0184630-mRNA-1:five_prime_utr;Parent=Msa0184630-mRNA-1
chr1_4 CDS 74187843 74187900 74187843 ID=Msa0184630-mRNA-1:cds;Parent=Msa0184630-mRNA-1
chr1_4 CDS 74188001 74188098 74188001 ID=Msa0184630-mRNA-1:cds;Parent=Msa0184630-mRNA-1
chr1_4 CDS 74188291 74188396 74188291 ID=Msa0184630-mRNA-1:cds;Parent=Msa0184630-mRNA-1
chr1_4 CDS 74189801 74190000 74189801 ID=Msa0184630-mRNA-1:cds;Parent=Msa0184630-mRNA-1
chr1_4 three_prime_UTR 74190001 74190566 74190001 ID=Msa0184630-mRNA-1:three_prime_utr;Parent=Msa0184630-mRNA-1
Gene Sequence

>Msa0184630

ATGGCAACCACGTTTAAAGGAATTTGTAAGAGTTTGAAGTACATTACTCAGATGTTTGTTGTGAAGGAGCGAGAGATGGAAATTGGGTACCCGACGGATGTTAAACATGTGGCTCATGTTGGATGGGATGGTCCCTCAGGGAATGGACCCAGTTGGATGGATGATTTTAAAAAAGTTCCTGAATTTTCGACATCAATTGGTAACTTGGATGAAATTAGAGACTCTGATCCTATGGCTGTCACTTCATTATGGTCCTCCATAGAAAATCAACAATCATCAAACATTTACAAAGGCATCTCATCTGCTGCAGGGGATCCTCATATTGCAGAGAAGCCTAAGCAGAAAAAGGTTAAGTCAACTTCCTCTTCCTGGTCACCATCATCTTCATCAAGACGATCAAGAGCATCAAAATCAAAGGCTACCTTCAATGAGAGAGAAGCAATACCAATTGCTCTGGTGTAG
Protein sequence

>Msa0184630

MATTFKGICKSLKYITQMFVVKEREMEIGYPTDVKHVAHVGWDGPSGNGPSWMDDFKKVPEFSTSIGNLDEIRDSDPMAVTSLWSSIENQQSSNIYKGISSAAGDPHIAEKPKQKKVKSTSSSWSPSSSSRRSRASKSKATFNEREAIPIALV*