AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0688870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688870 MtrunA17_Chr1g0205991 98.039 153 3 0 1 153 1 153 2.98e-109 307
Msa0688870 MtrunA17_Chr6g0452871 54.545 110 44 1 4 107 2 111 6.81e-37 124
Msa0688870 MtrunA17_Chr1g0183501 61.667 60 22 1 6 64 7 66 2.45e-22 85.1
Msa0688870 MtrunA17_Chr1g0166511 56.364 55 24 0 3 57 2 56 4.60e-19 79.7
Msa0688870 MtrunA17_Chr1g0187421 47.541 61 31 1 5 64 20 80 7.83e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688870 AT2G33460.1 38.194 144 81 3 1 136 1 144 5.49e-24 93.6
Msa0688870 AT4G28556.1 59.375 64 21 2 2 60 7 70 3.57e-21 85.9
Msa0688870 AT4G21745.1 50.000 92 42 3 1 91 1 89 4.33e-20 81.6
Msa0688870 AT1G04450.1 63.636 55 19 1 4 57 3 57 9.80e-20 82.0
Msa0688870 AT3G23380.1 60.345 58 22 1 1 57 1 58 2.41e-19 80.5
Msa0688870 AT3G23380.2 60.345 58 22 1 1 57 1 58 2.41e-19 80.5
Msa0688870 AT4G04900.2 61.017 59 21 2 1 57 1 59 1.61e-18 77.4
Msa0688870 AT4G04900.1 61.017 59 21 2 1 57 1 59 1.61e-18 77.4
Msa0688870 AT2G20430.1 50.725 69 31 2 2 67 7 75 4.69e-17 75.1
Msa0688870 AT1G03982.1 62.264 53 17 2 22 71 11 63 1.90e-13 64.7
Msa0688870 AT4G28556.2 72.973 37 8 1 26 60 1 37 2.52e-13 64.7
Msa0688870 AT1G61795.1 57.627 59 23 2 1 57 1 59 5.91e-13 62.4
Msa0688870 AT3G23380.3 75.000 32 8 0 26 57 1 32 3.35e-12 61.2

Find 100 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTGGTTATTCACTTATTC+TGG 0.147150 4_4:-55140810 Msa0688870:three_prime_UTR
TTGATACTCTACAACGTTAT+TGG 0.154823 4_4:-55143285 Msa0688870:five_prime_UTR
ACTTTATACGGCATTATTAT+TGG 0.165637 4_4:-55143212 Msa0688870:five_prime_UTR
ATCTTTCTTTCCCTGGTTAC+TGG 0.192124 4_4:+55140951 None:intergenic
ACCATGGCAACCACGTTTAA+AGG 0.192767 4_4:-55143176 Msa0688870:CDS
ATATCTTTCAAATACTAAAA+TGG 0.194485 4_4:+55140495 None:intergenic
AAGGAGCGAGAGATGGAAAT+TGG 0.205114 4_4:-55143010 Msa0688870:CDS
TTGTCTGCAAACGAATAATT+TGG 0.235287 4_4:+55140857 None:intergenic
CCAATTGATGTCGAAAATTC+AGG 0.256562 4_4:+55142704 None:intergenic
GGAAATTTGTAATGAGTGTT+TGG 0.278209 4_4:-55140784 Msa0688870:three_prime_UTR
CTTTATACGGCATTATTATT+GGG 0.289092 4_4:-55143211 Msa0688870:five_prime_UTR
TAGGAAATTAGAGAGATATT+TGG 0.310626 4_4:-55141003 Msa0688870:three_prime_UTR
GCACTGAGGTGCTCAAAATA+TGG 0.342460 4_4:-55140608 Msa0688870:three_prime_UTR
AAAGAATACTTTGTTTCATA+TGG 0.347226 4_4:+55140975 None:intergenic
TTAAGTCAACTTCCTCTTCC+TGG 0.354143 4_4:-55141128 Msa0688870:CDS
ACCTCAGTGCATGCTCAAAA+TGG 0.354398 4_4:+55140621 None:intergenic
TTCAAGGTCAGCATATGATT+TGG 0.357796 4_4:+55140755 None:intergenic
AGAGAAGCCTAAGCAGAAAA+AGG 0.371668 4_4:-55141151 Msa0688870:CDS
GGAAAGAAAGATAATAAATA+TGG 0.375964 4_4:-55140940 Msa0688870:three_prime_UTR
GTAAAGGTAAGGAAAATCTA+TGG 0.398328 4_4:+55143529 None:intergenic
ATTTATTATCTTTCTTTCCC+TGG 0.401689 4_4:+55140944 None:intergenic
GTTGGATGGGATGGTCCCTC+AGG 0.402348 4_4:-55142956 Msa0688870:CDS
GAAATTTGTAATGAGTGTTT+GGG 0.403659 4_4:-55140783 Msa0688870:three_prime_UTR
GAAAATCTATGGATATGAAA+TGG 0.404473 4_4:+55143540 None:intergenic
TTCGACATCAATTGGTAACT+TGG 0.415150 4_4:-55142696 Msa0688870:CDS
TATCTTTCAAATACTAAAAT+GGG 0.418115 4_4:+55140496 None:intergenic
TATTGCTTCTCTCTCATTGA+AGG 0.419651 4_4:+55141056 None:intergenic
CGTGGTTATTCACTTATTCT+GGG 0.424875 4_4:-55140809 Msa0688870:three_prime_UTR
CGACACAACTTATAAGTTCA+AGG 0.426916 4_4:+55140739 None:intergenic
AAGTACATTACTCAGATGTT+TGG 0.428512 4_4:-55143137 Msa0688870:intron
TGAAAGTAGGAGGTGGAAGA+AGG 0.428583 4_4:+55143631 None:intergenic
ATCTGCTACACCAGAGCAAT+TGG 0.434357 4_4:+55141033 None:intergenic
ATATAAACACCATACCCAAC+TGG 0.441516 4_4:+55142925 None:intergenic
CTATGGCTGTCACTTCATTA+TGG 0.445205 4_4:-55142652 Msa0688870:CDS
TCCTTTAAACGTGGTTGCCA+TGG 0.449019 4_4:+55143175 None:intergenic
CTCAGGGAATGGACCCAGTT+GGG 0.465751 4_4:-55142939 Msa0688870:intron
GAAAGTAGGAGGTGGAAGAA+GGG 0.466205 4_4:+55143632 None:intergenic
GAACAAATGAGAAAATCATC+TGG 0.466403 4_4:+55140472 None:intergenic
AAGAAGGGTTTGACTCTAGA+AGG 0.469038 4_4:+55143647 None:intergenic
TGGGATGGTCCCTCAGGGAA+TGG 0.472703 4_4:-55142950 Msa0688870:CDS
AAAGGCATCTCATCTGCTGC+AGG 0.474060 4_4:-55141189 Msa0688870:CDS
TCTTTCTTTCCCTGGTTACT+GGG 0.477566 4_4:+55140952 None:intergenic
AGTATTCTTTCCCAGTAACC+AGG 0.482131 4_4:-55140962 Msa0688870:three_prime_UTR
AATGGAAGAAAATATGTAAT+GGG 0.485099 4_4:+55143568 None:intergenic
TTAGGCTTCTCTGCAATATG+AGG 0.487591 4_4:+55141162 None:intergenic
GAAGGAGGTTGAGAAGAGAA+GGG 0.491876 4_4:+55143665 None:intergenic
AGGTATTTGCAGCGTTCAGA+TGG 0.493670 4_4:-55140582 Msa0688870:three_prime_UTR
AGAAGGAGGTTGAGAAGAGA+AGG 0.499131 4_4:+55143664 None:intergenic
AGGTGCTCAAAATATGGAAA+AGG 0.503103 4_4:-55140602 Msa0688870:three_prime_UTR
AGGAGCGAGAGATGGAAATT+GGG 0.509528 4_4:-55143009 Msa0688870:CDS
AATTAGAGACTCTGATCCTA+TGG 0.510482 4_4:-55142669 Msa0688870:CDS
GTTATTCACTTATTCTGGGA+AGG 0.514220 4_4:-55140805 Msa0688870:three_prime_UTR
AGAAGCAATACCAATTGCTC+TGG 0.524560 4_4:-55141043 Msa0688870:CDS
TCTATGGATATGAAATGGAA+TGG 0.526863 4_4:+55143545 None:intergenic
GTGGCTCATGTTGGATGGGA+TGG 0.528972 4_4:-55142965 Msa0688870:CDS
CATAATGAAGTGACAGCCAT+AGG 0.529806 4_4:+55142653 None:intergenic
GAATGGAAGAAAATATGTAA+TGG 0.537302 4_4:+55143567 None:intergenic
AGTTGTGAAGGAGCGAGAGA+TGG 0.540742 4_4:-55143017 Msa0688870:CDS
TAATTTACTAACTAGAAGCC+AGG 0.542740 4_4:+55140904 None:intergenic
AGGAGGTTGAGAAGAGAAGG+GGG 0.543162 4_4:+55143667 None:intergenic
ATTCATGTTGAAACATGTAA+AGG 0.543248 4_4:+55143513 None:intergenic
TGTTGAAACATGTAAAGGTA+AGG 0.543333 4_4:+55143518 None:intergenic
GGATATGAAATGGAATGGAA+TGG 0.544274 4_4:+55143550 None:intergenic
AAGGAGGTTGAGAAGAGAAG+GGG 0.549181 4_4:+55143666 None:intergenic
ATCAAGAGCATCAAAATCAA+AGG 0.551691 4_4:-55141082 Msa0688870:CDS
ATGAAAGTGAAAGTAGGAGG+TGG 0.552421 4_4:+55143624 None:intergenic
ACATGAATTTCAAAACCTGC+AGG 0.552558 4_4:-55143498 Msa0688870:intron
AGGCATCTCATCTGCTGCAG+GGG 0.558500 4_4:-55141187 Msa0688870:CDS
AACATGTGGCTCATGTTGGA+TGG 0.559701 4_4:-55142970 Msa0688870:CDS
AAATTATAATCTAGTCCAGT+GGG 0.561409 4_4:-55140887 Msa0688870:three_prime_UTR
AGATGATGGTGACCAGGAAG+AGG 0.563076 4_4:+55141116 None:intergenic
GAAGATTTAAAGGAATGGCA+TGG 0.568361 4_4:-55140708 Msa0688870:three_prime_UTR
GGTGTAGCAGATTCAAGACT+AGG 0.573682 4_4:-55141022 Msa0688870:exon
GTTAAACATGTGGCTCATGT+TGG 0.581035 4_4:-55142974 Msa0688870:CDS
CCTCAGGGAATGGACCCAGT+TGG 0.581964 4_4:-55142940 Msa0688870:CDS
ACGTTGTAGAGTATCAACTA+AGG 0.582432 4_4:+55143291 None:intergenic
ACTTCATTATGGTCCTCCAT+AGG 0.586915 4_4:-55142641 Msa0688870:intron
CCACATGTTTAACATCCGTC+GGG 0.587906 4_4:+55142984 None:intergenic
AAGGCATCTCATCTGCTGCA+GGG 0.590720 4_4:-55141188 Msa0688870:CDS
GCCACATGTTTAACATCCGT+CGG 0.595209 4_4:+55142983 None:intergenic
TATAAACACCATACCCAACT+GGG 0.596139 4_4:+55142926 None:intergenic
CTTACAAATTCCTTTAAACG+TGG 0.598013 4_4:+55143166 None:intergenic
ATGCACAGATAAACTCTACA+AGG 0.609804 4_4:+55140558 None:intergenic
TTGGATGGGATGGTCCCTCA+GGG 0.611512 4_4:-55142955 Msa0688870:CDS
ACATGTGGCTCATGTTGGAT+GGG 0.613430 4_4:-55142969 Msa0688870:CDS
TGATGAAGATGATGGTGACC+AGG 0.620233 4_4:+55141110 None:intergenic
GATCGTCTTGATGAAGATGA+TGG 0.625529 4_4:+55141102 None:intergenic
TAAATTATAATCTAGTCCAG+TGG 0.627743 4_4:-55140888 Msa0688870:three_prime_UTR
ATAGTTGTATGTCCGTCCTG+TGG 0.632873 4_4:-55140676 Msa0688870:three_prime_UTR
TGGGATTGTGATCTTCACCA+TGG 0.633325 4_4:-55143192 Msa0688870:exon
AAGGGTTTGACTCTAGAAGG+AGG 0.633887 4_4:+55143650 None:intergenic
CAATCATCAAACATTTACAA+AGG 0.636264 4_4:-55141207 Msa0688870:CDS
GTATTCTTTCCCAGTAACCA+GGG 0.643305 4_4:-55140961 Msa0688870:three_prime_UTR
GAAGCAAAGCATACCTATGG+AGG 0.644836 4_4:+55142628 None:intergenic
GGTATGAAAGTGAAAGTAGG+AGG 0.655406 4_4:+55143621 None:intergenic
CCCGACGGATGTTAAACATG+TGG 0.657287 4_4:-55142984 Msa0688870:CDS
GATGGAAATTGGGTACCCGA+CGG 0.676519 4_4:-55142999 Msa0688870:CDS
CAACTGGGTCCATTCCCTGA+GGG 0.690377 4_4:+55142941 None:intergenic
GATTTAAAGGAATGGCATGG+TGG 0.699903 4_4:-55140705 Msa0688870:three_prime_UTR
CCAACTGGGTCCATTCCCTG+AGG 0.728969 4_4:+55142940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGATTATTGAAAATTATT+GGG - chr4_4:55142471-55142490 Msa0688870:intron 10.0%
!! ATGTAAAATGATAATTTCTA+CGG + chr4_4:55140735-55140754 None:intergenic 15.0%
!! AAAATAATATACAAAATGTC+TGG + chr4_4:55141372-55141391 None:intergenic 15.0%
!! TATTAAAAATTTGATAGAGA+TGG - chr4_4:55141914-55141933 Msa0688870:intron 15.0%
!!! CATATTTTAAAATTTACTTC+AGG - chr4_4:55142444-55142463 Msa0688870:intron 15.0%
!!! ATAATTTTCAATAATCAATG+AGG + chr4_4:55142470-55142489 None:intergenic 15.0%
!!! CATTGATTATTGAAAATTAT+TGG - chr4_4:55142470-55142489 Msa0688870:intron 15.0%
!! TATCTTTCAAATACTAAAAT+GGG + chr4_4:55143651-55143670 None:intergenic 15.0%
!! ATATCTTTCAAATACTAAAA+TGG + chr4_4:55143652-55143671 None:intergenic 15.0%
!! AATGGAAGAAAATATGTAAT+GGG + chr4_4:55140579-55140598 None:intergenic 20.0%
!!! TTTTGTGTCTTTACTTTATA+CGG - chr4_4:55140920-55140939 Msa0688870:three_prime_UTR 20.0%
!! ATCACATTAAACACATTAAT+TGG + chr4_4:55141057-55141076 None:intergenic 20.0%
!! TTTGGACTAAAATATGAAAT+GGG - chr4_4:55141095-55141114 Msa0688870:CDS 20.0%
!!! CAATTTCTCTTCTTTTTTTA+AGG + chr4_4:55141865-55141884 None:intergenic 20.0%
!! AAAACAATCTATATATTCCA+AGG - chr4_4:55142176-55142195 Msa0688870:intron 20.0%
!! AAACAATCTATATATTCCAA+GGG - chr4_4:55142177-55142196 Msa0688870:intron 20.0%
!!! AAAGACAGATAAATTTTTGT+TGG + chr4_4:55142380-55142399 None:intergenic 20.0%
!! ATTATTGAAAATTATTGGGT+TGG - chr4_4:55142475-55142494 Msa0688870:intron 20.0%
!!! TAAACACAAATTTTAGCATA+TGG + chr4_4:55142554-55142573 None:intergenic 20.0%
!!! ATATTTTGAAACCTAAATTC+TGG + chr4_4:55142583-55142602 None:intergenic 20.0%
!! AGAATTTAATCAGTATTGTA+TGG + chr4_4:55142682-55142701 None:intergenic 20.0%
!! GAAATAAATAAATTTGTCCA+TGG + chr4_4:55142737-55142756 None:intergenic 20.0%
!!! ATCAGATCTTATTTGTTATT+TGG - chr4_4:55142860-55142879 Msa0688870:intron 20.0%
!!! AAAGAATACTTTGTTTCATA+TGG + chr4_4:55143172-55143191 None:intergenic 20.0%
!! GGAAAGAAAGATAATAAATA+TGG - chr4_4:55143204-55143223 Msa0688870:five_prime_UTR 20.0%
!!! ATTTTGAAGATTTAAAGGAA+TGG - chr4_4:55143431-55143450 Msa0688870:intron 20.0%
!!! AAGTTGAGATTTTGATTAGT+GGG + chr4_4:55140549-55140568 None:intergenic 25.0%
!!! AAAGTTGAGATTTTGATTAG+TGG + chr4_4:55140550-55140569 None:intergenic 25.0%
! GAATGGAAGAAAATATGTAA+TGG + chr4_4:55140580-55140599 None:intergenic 25.0%
! GAAAATCTATGGATATGAAA+TGG + chr4_4:55140607-55140626 None:intergenic 25.0%
! ATTCATGTTGAAACATGTAA+AGG + chr4_4:55140634-55140653 None:intergenic 25.0%
! ACTTTATACGGCATTATTAT+TGG - chr4_4:55140932-55140951 Msa0688870:three_prime_UTR 25.0%
! CTTTATACGGCATTATTATT+GGG - chr4_4:55140933-55140952 Msa0688870:three_prime_UTR 25.0%
!!! TTCTGTGTTTTGTTATTGTT+TGG - chr4_4:55141077-55141096 Msa0688870:CDS 25.0%
! GTTTGGACTAAAATATGAAA+TGG - chr4_4:55141094-55141113 Msa0688870:CDS 25.0%
!!! CTTTTTTAAAATCATCCATC+TGG + chr4_4:55141418-55141437 None:intergenic 25.0%
!! TTGATTATTGTTGTGTGTTT+TGG - chr4_4:55141594-55141613 Msa0688870:intron 25.0%
!!! TAACACTGTTTTTTTAAGTG+TGG - chr4_4:55141754-55141773 Msa0688870:intron 25.0%
!! TTGTTAAGTACATGTTTTGT+TGG - chr4_4:55141792-55141811 Msa0688870:intron 25.0%
! TATTGATAAAGCTTATCACA+TGG + chr4_4:55141830-55141849 None:intergenic 25.0%
! ATCATGACTACATAAATACA+AGG + chr4_4:55142015-55142034 None:intergenic 25.0%
! TGTATTTATGTAGTCATGAT+TGG - chr4_4:55142015-55142034 Msa0688870:intron 25.0%
! TAAGAAGTTAACATGAAACA+AGG - chr4_4:55142269-55142288 Msa0688870:intron 25.0%
!! ACAGATAAATTTTTGTTGGT+GGG + chr4_4:55142376-55142395 None:intergenic 25.0%
! GTTTAATAACTCCAGAATTT+AGG - chr4_4:55142569-55142588 Msa0688870:intron 25.0%
!! AAATATACTGTTTAGCTTCA+TGG - chr4_4:55142597-55142616 Msa0688870:intron 25.0%
! GAATAGGTATCATTAAGAAT+AGG + chr4_4:55142899-55142918 None:intergenic 25.0%
! CAATCATCAAACATTTACAA+AGG - chr4_4:55142937-55142956 Msa0688870:intron 25.0%
! TAGGAAATTAGAGAGATATT+TGG - chr4_4:55143141-55143160 Msa0688870:CDS 25.0%
! ATTTATTATCTTTCTTTCCC+TGG + chr4_4:55143203-55143222 None:intergenic 25.0%
! TAAATTATAATCTAGTCCAG+TGG - chr4_4:55143256-55143275 Msa0688870:five_prime_UTR 25.0%
! AAATTATAATCTAGTCCAGT+GGG - chr4_4:55143257-55143276 Msa0688870:five_prime_UTR 25.0%
! GAAATTTGTAATGAGTGTTT+GGG - chr4_4:55143361-55143380 Msa0688870:intron 25.0%
!!! CGTCAATTTTGAAGATTTAA+AGG - chr4_4:55143426-55143445 Msa0688870:intron 25.0%
!!! AGTTGAGATTTTGATTAGTG+GGG + chr4_4:55140548-55140567 None:intergenic 30.0%
TCTATGGATATGAAATGGAA+TGG + chr4_4:55140602-55140621 None:intergenic 30.0%
GTAAAGGTAAGGAAAATCTA+TGG + chr4_4:55140618-55140637 None:intergenic 30.0%
TGTTGAAACATGTAAAGGTA+AGG + chr4_4:55140629-55140648 None:intergenic 30.0%
TTGATACTCTACAACGTTAT+TGG - chr4_4:55140859-55140878 Msa0688870:three_prime_UTR 30.0%
CTTACAAATTCCTTTAAACG+TGG + chr4_4:55140981-55141000 None:intergenic 30.0%
AAGTACATTACTCAGATGTT+TGG - chr4_4:55141007-55141026 Msa0688870:three_prime_UTR 30.0%
GGTATGGTGTTTATATAGAT+TGG - chr4_4:55141226-55141245 Msa0688870:intron 30.0%
! TACAAAATGTCTGGAATCAT+CGG + chr4_4:55141363-55141382 None:intergenic 30.0%
ACAAATGTATTGTCCTTTAC+CGG - chr4_4:55141543-55141562 Msa0688870:intron 30.0%
!!! CATTTTTTCGACTAATTGAC+CGG + chr4_4:55141565-55141584 None:intergenic 30.0%
! ATGTTCTTGATAAAAGTGTG+AGG - chr4_4:55141619-55141638 Msa0688870:intron 30.0%
AACCATAAGATTCTCTTTGT+TGG + chr4_4:55141718-55141737 None:intergenic 30.0%
!! TACATGTTTTGTTGGTACTT+TGG - chr4_4:55141800-55141819 Msa0688870:intron 30.0%
TACATAAATACAAGGCTAGT+TGG + chr4_4:55142007-55142026 None:intergenic 30.0%
TTTATGTAGTCATGATTGGA+TGG - chr4_4:55142019-55142038 Msa0688870:intron 30.0%
ATGATTGGATGGATGTTTAA+AGG - chr4_4:55142030-55142049 Msa0688870:intron 30.0%
AGATACTTTGTCTTTCTTGA+AGG - chr4_4:55142120-55142139 Msa0688870:intron 30.0%
GATACTTTGTCTTTCTTGAA+GGG - chr4_4:55142121-55142140 Msa0688870:intron 30.0%
ATACTTTGTCTTTCTTGAAG+GGG - chr4_4:55142122-55142141 Msa0688870:intron 30.0%
AATCTATATATTCCAAGGGT+GGG - chr4_4:55142181-55142200 Msa0688870:intron 30.0%
TTAGTCAGATACTCCAAATT+TGG + chr4_4:55142214-55142233 None:intergenic 30.0%
TTCTGTTTGAAAGATAACAG+TGG - chr4_4:55142300-55142319 Msa0688870:intron 30.0%
! GACAGATAAATTTTTGTTGG+TGG + chr4_4:55142377-55142396 None:intergenic 30.0%
TGTAATCACAATGCAATTGT+TGG + chr4_4:55142759-55142778 None:intergenic 30.0%
TTACATTTCCTTCCATTTCT+AGG - chr4_4:55142774-55142793 Msa0688870:intron 30.0%
GTATCATTAAGAATAGGAAC+TGG + chr4_4:55142893-55142912 None:intergenic 30.0%
!! ATCAAGAGCATCAAAATCAA+AGG - chr4_4:55143062-55143081 Msa0688870:intron 30.0%
TAATTTACTAACTAGAAGCC+AGG + chr4_4:55143243-55143262 None:intergenic 30.0%
TTGTCTGCAAACGAATAATT+TGG + chr4_4:55143290-55143309 None:intergenic 30.0%
CAATGAAAAAAACACTAACG+TGG - chr4_4:55143317-55143336 Msa0688870:five_prime_UTR 30.0%
GGAAATTTGTAATGAGTGTT+TGG - chr4_4:55143360-55143379 Msa0688870:intron 30.0%
GAACAAATGAGAAAATCATC+TGG + chr4_4:55143675-55143694 None:intergenic 30.0%
!!! GTTGAGATTTTGATTAGTGG+GGG + chr4_4:55140547-55140566 None:intergenic 35.0%
GGATATGAAATGGAATGGAA+TGG + chr4_4:55140597-55140616 None:intergenic 35.0%
ACATGAATTTCAAAACCTGC+AGG - chr4_4:55140646-55140665 Msa0688870:three_prime_UTR 35.0%
AGATGCTTATCTTTACTCAC+AGG - chr4_4:55140788-55140807 Msa0688870:three_prime_UTR 35.0%
ACTCACAGGAAAACAAAAAG+AGG - chr4_4:55140802-55140821 Msa0688870:three_prime_UTR 35.0%
! ACGTTGTAGAGTATCAACTA+AGG + chr4_4:55140856-55140875 None:intergenic 35.0%
TATAAACACCATACCCAACT+GGG + chr4_4:55141221-55141240 None:intergenic 35.0%
ATATAAACACCATACCCAAC+TGG + chr4_4:55141222-55141241 None:intergenic 35.0%
! ACAAAATGTCTGGAATCATC+GGG + chr4_4:55141362-55141381 None:intergenic 35.0%
! AAATCATCCATCTGGAATCA+CGG + chr4_4:55141410-55141429 None:intergenic 35.0%
CCAATTGATGTCGAAAATTC+AGG + chr4_4:55141443-55141462 None:intergenic 35.0%
! CCTGAATTTTCGACATCAAT+TGG - chr4_4:55141440-55141459 Msa0688870:intron 35.0%
! TTCGACATCAATTGGTAACT+TGG - chr4_4:55141448-55141467 Msa0688870:intron 35.0%
AATTAGAGACTCTGATCCTA+TGG - chr4_4:55141475-55141494 Msa0688870:intron 35.0%
AACAGCCAAACTCTTTCTAA+GGG + chr4_4:55141674-55141693 None:intergenic 35.0%
CTCCAACAAAGAGAATCTTA+TGG - chr4_4:55141713-55141732 Msa0688870:intron 35.0%
CATGTACTTAACAAGCATTC+TGG + chr4_4:55141786-55141805 None:intergenic 35.0%
TGTCACGTTCTAGTATGAAT+TGG - chr4_4:55141959-55141978 Msa0688870:intron 35.0%
! TAAATACAAGGCTAGTTGGA+AGG + chr4_4:55142003-55142022 None:intergenic 35.0%
!! GCAAAGCTGATTGTTTTTCT+TGG + chr4_4:55142071-55142090 None:intergenic 35.0%
ACAGTCATGGCTAATTCTTA+AGG + chr4_4:55142095-55142114 None:intergenic 35.0%
CAATCTATATATTCCAAGGG+TGG - chr4_4:55142180-55142199 Msa0688870:intron 35.0%
! TGGTGATTATCCTTTTCTGA+AGG - chr4_4:55142617-55142636 Msa0688870:intron 35.0%
ATTCTCATCCTAGAAATGGA+AGG + chr4_4:55142785-55142804 None:intergenic 35.0%
CATGATTCTCATCCTAGAAA+TGG + chr4_4:55142789-55142808 None:intergenic 35.0%
ATTAAGAATAGGAACTGGCA+TGG + chr4_4:55142888-55142907 None:intergenic 35.0%
! GATTTTCTGTGCACAAGAAT+AGG + chr4_4:55142915-55142934 None:intergenic 35.0%
! GACTTAACCTTTTTCTGCTT+AGG + chr4_4:55143003-55143022 None:intergenic 35.0%
TATTGCTTCTCTCTCATTGA+AGG + chr4_4:55143091-55143110 None:intergenic 35.0%
! TATGGCAGTGAAAATTTTCC+TGG - chr4_4:55143222-55143241 Msa0688870:five_prime_UTR 35.0%
!!! TAATTTGGTTTTACTCCCAC+TGG + chr4_4:55143275-55143294 None:intergenic 35.0%
ACGTGGTTATTCACTTATTC+TGG - chr4_4:55143334-55143353 Msa0688870:five_prime_UTR 35.0%
CGTGGTTATTCACTTATTCT+GGG - chr4_4:55143335-55143354 Msa0688870:five_prime_UTR 35.0%
! GTTATTCACTTATTCTGGGA+AGG - chr4_4:55143339-55143358 Msa0688870:intron 35.0%
TTCAAGGTCAGCATATGATT+TGG + chr4_4:55143392-55143411 None:intergenic 35.0%
CGACACAACTTATAAGTTCA+AGG + chr4_4:55143408-55143427 None:intergenic 35.0%
GAAGATTTAAAGGAATGGCA+TGG - chr4_4:55143436-55143455 Msa0688870:intron 35.0%
AACAAGAAAAAACATGCCAC+AGG + chr4_4:55143487-55143506 None:intergenic 35.0%
AGGTGCTCAAAATATGGAAA+AGG - chr4_4:55143542-55143561 Msa0688870:five_prime_UTR 35.0%
ATGCACAGATAAACTCTACA+AGG + chr4_4:55143589-55143608 None:intergenic 35.0%
AAGAAGGGTTTGACTCTAGA+AGG + chr4_4:55140500-55140519 None:intergenic 40.0%
ATGAAAGTGAAAGTAGGAGG+TGG + chr4_4:55140523-55140542 None:intergenic 40.0%
GGTATGAAAGTGAAAGTAGG+AGG + chr4_4:55140526-55140545 None:intergenic 40.0%
GAGTATAGTATAGTACCTGC+AGG + chr4_4:55140664-55140683 None:intergenic 40.0%
GTTAAACATGTGGCTCATGT+TGG - chr4_4:55141170-55141189 Msa0688870:CDS 40.0%
CATAATGAAGTGACAGCCAT+AGG + chr4_4:55141494-55141513 None:intergenic 40.0%
! CTATGGCTGTCACTTCATTA+TGG - chr4_4:55141492-55141511 Msa0688870:intron 40.0%
ACTTCATTATGGTCCTCCAT+AGG - chr4_4:55141503-55141522 Msa0688870:intron 40.0%
CAAGAAGCAAAGCATACCTA+TGG + chr4_4:55141522-55141541 None:intergenic 40.0%
TTCGACTAATTGACCGGTAA+AGG + chr4_4:55141559-55141578 None:intergenic 40.0%
TTGCTCCCTTAGAAAGAGTT+TGG - chr4_4:55141666-55141685 Msa0688870:intron 40.0%
GAACAGCCAAACTCTTTCTA+AGG + chr4_4:55141675-55141694 None:intergenic 40.0%
ACTTAACAAGCATTCTGGTG+TGG + chr4_4:55141781-55141800 None:intergenic 40.0%
ACAGCAATGTCTACTAGTGA+TGG + chr4_4:55141942-55141961 None:intergenic 40.0%
AAGTATCTCAGTCACAGTCA+TGG + chr4_4:55142108-55142127 None:intergenic 40.0%
AAAGATAACAGTGGCACTCA+GGG - chr4_4:55142309-55142328 Msa0688870:intron 40.0%
TTAGGCTTCTCTGCAATATG+AGG + chr4_4:55142985-55143004 None:intergenic 40.0%
AGAGAAGCCTAAGCAGAAAA+AGG - chr4_4:55142993-55143012 Msa0688870:CDS 40.0%
TTAAGTCAACTTCCTCTTCC+TGG - chr4_4:55143016-55143035 Msa0688870:CDS 40.0%
GATCGTCTTGATGAAGATGA+TGG + chr4_4:55143045-55143064 None:intergenic 40.0%
AGAAGCAATACCAATTGCTC+TGG - chr4_4:55143101-55143120 Msa0688870:intron 40.0%
AGTATTCTTTCCCAGTAACC+AGG - chr4_4:55143182-55143201 Msa0688870:exon 40.0%
GTATTCTTTCCCAGTAACCA+GGG - chr4_4:55143183-55143202 Msa0688870:exon 40.0%
TCTTTCTTTCCCTGGTTACT+GGG + chr4_4:55143195-55143214 None:intergenic 40.0%
ATCTTTCTTTCCCTGGTTAC+TGG + chr4_4:55143196-55143215 None:intergenic 40.0%
GATTTAAAGGAATGGCATGG+TGG - chr4_4:55143439-55143458 Msa0688870:intron 40.0%
AGAAAAAACATGCCACAGGA+CGG + chr4_4:55143483-55143502 None:intergenic 40.0%
AAGGAGGTTGAGAAGAGAAG+GGG + chr4_4:55140481-55140500 None:intergenic 45.0%
GAAGGAGGTTGAGAAGAGAA+GGG + chr4_4:55140482-55140501 None:intergenic 45.0%
AGAAGGAGGTTGAGAAGAGA+AGG + chr4_4:55140483-55140502 None:intergenic 45.0%
! AAGGGTTTGACTCTAGAAGG+AGG + chr4_4:55140497-55140516 None:intergenic 45.0%
GAAAGTAGGAGGTGGAAGAA+GGG + chr4_4:55140515-55140534 None:intergenic 45.0%
TGAAAGTAGGAGGTGGAAGA+AGG + chr4_4:55140516-55140535 None:intergenic 45.0%
! GGGGGTATGAAAGTGAAAGT+AGG + chr4_4:55140529-55140548 None:intergenic 45.0%
TGGGATTGTGATCTTCACCA+TGG - chr4_4:55140952-55140971 Msa0688870:three_prime_UTR 45.0%
ACCATGGCAACCACGTTTAA+AGG - chr4_4:55140968-55140987 Msa0688870:three_prime_UTR 45.0%
TCCTTTAAACGTGGTTGCCA+TGG + chr4_4:55140972-55140991 None:intergenic 45.0%
AAGGAGCGAGAGATGGAAAT+TGG - chr4_4:55141134-55141153 Msa0688870:CDS 45.0%
AGGAGCGAGAGATGGAAATT+GGG - chr4_4:55141135-55141154 Msa0688870:CDS 45.0%
CCACATGTTTAACATCCGTC+GGG + chr4_4:55141163-55141182 None:intergenic 45.0%
GCCACATGTTTAACATCCGT+CGG + chr4_4:55141164-55141183 None:intergenic 45.0%
AACATGTGGCTCATGTTGGA+TGG - chr4_4:55141174-55141193 Msa0688870:CDS 45.0%
ACATGTGGCTCATGTTGGAT+GGG - chr4_4:55141175-55141194 Msa0688870:CDS 45.0%
! TTTTATGCTGCGCGCATGTT+TGG - chr4_4:55141285-55141304 Msa0688870:intron 45.0%
GTTTGGATTCACGGTGAGTT+TGG - chr4_4:55141302-55141321 Msa0688870:intron 45.0%
! TTTTCTGCCGTGATTCCAGA+TGG - chr4_4:55141400-55141419 Msa0688870:intron 45.0%
GAAGCAAAGCATACCTATGG+AGG + chr4_4:55141519-55141538 None:intergenic 45.0%
! ATACAAGGCTAGTTGGAAGG+CGG + chr4_4:55142000-55142019 None:intergenic 45.0%
GAAAGATAACAGTGGCACTC+AGG - chr4_4:55142308-55142327 Msa0688870:intron 45.0%
! TTTAGTGGCCTTACACCCTT+AGG + chr4_4:55142339-55142358 None:intergenic 45.0%
TGCTCCGTATGCAGACAAAA+AGG - chr4_4:55142418-55142437 Msa0688870:intron 45.0%
! GCGACCTTTTTGTCTGCATA+CGG + chr4_4:55142425-55142444 None:intergenic 45.0%
!! GAAAATTATTGGGTTGGCCC+TGG - chr4_4:55142481-55142500 Msa0688870:intron 45.0%
ATGCGTAGAGCCTTCAGAAA+AGG + chr4_4:55142630-55142649 None:intergenic 45.0%
AAATGCATTGCGTCGTACCA+TGG - chr4_4:55142717-55142736 Msa0688870:CDS 45.0%
! TGATGAAGATGATGGTGACC+AGG + chr4_4:55143037-55143056 None:intergenic 45.0%
ATCTGCTACACCAGAGCAAT+TGG + chr4_4:55143114-55143133 None:intergenic 45.0%
GGTGTAGCAGATTCAAGACT+AGG - chr4_4:55143122-55143141 Msa0688870:intron 45.0%
ATAGTTGTATGTCCGTCCTG+TGG - chr4_4:55143468-55143487 Msa0688870:intron 45.0%
! TCCATTTTGAGCATGCACTG+AGG - chr4_4:55143522-55143541 Msa0688870:five_prime_UTR 45.0%
ACCTCAGTGCATGCTCAAAA+TGG + chr4_4:55143526-55143545 None:intergenic 45.0%
! GCACTGAGGTGCTCAAAATA+TGG - chr4_4:55143536-55143555 Msa0688870:five_prime_UTR 45.0%
AGGTATTTGCAGCGTTCAGA+TGG - chr4_4:55143562-55143581 Msa0688870:five_prime_UTR 45.0%
AGGAGGTTGAGAAGAGAAGG+GGG + chr4_4:55140480-55140499 None:intergenic 50.0%
! GGGTCTTTTCCCAGTTGTGA+AGG - chr4_4:55141115-55141134 Msa0688870:CDS 50.0%
TCTCTCGCTCCTTCACAACT+GGG + chr4_4:55141127-55141146 None:intergenic 50.0%
ATCTCTCGCTCCTTCACAAC+TGG + chr4_4:55141128-55141147 None:intergenic 50.0%
AGTTGTGAAGGAGCGAGAGA+TGG - chr4_4:55141127-55141146 Msa0688870:CDS 50.0%
GATGGAAATTGGGTACCCGA+CGG - chr4_4:55141145-55141164 Msa0688870:CDS 50.0%
CCCGACGGATGTTAAACATG+TGG - chr4_4:55141160-55141179 Msa0688870:CDS 50.0%
TGCGCGCATGTTTGGATTCA+CGG - chr4_4:55141293-55141312 Msa0688870:intron 50.0%
GGTGGGTCTAGTGCCAAATT+TGG - chr4_4:55142198-55142217 Msa0688870:intron 50.0%
! GCCACTAAAGCCCTAGCTTT+AGG - chr4_4:55142350-55142369 Msa0688870:intron 50.0%
! GCCTAAAGCTAGGGCTTTAG+TGG + chr4_4:55142354-55142373 None:intergenic 50.0%
! TGTTGGTGGGCCTAAAGCTA+GGG + chr4_4:55142363-55142382 None:intergenic 50.0%
! TTGTTGGTGGGCCTAAAGCT+AGG + chr4_4:55142364-55142383 None:intergenic 50.0%
!! AATTATTGGGTTGGCCCTGG+CGG - chr4_4:55142484-55142503 Msa0688870:intron 50.0%
CTGCATGTTACCTTCGACTG+TGG - chr4_4:55142653-55142672 Msa0688870:CDS 50.0%
TGTATGGTGACCACAGTCGA+AGG + chr4_4:55142666-55142685 None:intergenic 50.0%
!! AAAGGCATCTCATCTGCTGC+AGG - chr4_4:55142955-55142974 Msa0688870:CDS 50.0%
! AAGGCATCTCATCTGCTGCA+GGG - chr4_4:55142956-55142975 Msa0688870:CDS 50.0%
! AGATGATGGTGACCAGGAAG+AGG + chr4_4:55143031-55143050 None:intergenic 50.0%
GTGGCTCATGTTGGATGGGA+TGG - chr4_4:55141179-55141198 Msa0688870:CDS 55.0%
TTGGATGGGATGGTCCCTCA+GGG - chr4_4:55141189-55141208 Msa0688870:CDS 55.0%
CAACTGGGTCCATTCCCTGA+GGG + chr4_4:55141206-55141225 None:intergenic 55.0%
CTCAGGGAATGGACCCAGTT+GGG - chr4_4:55141205-55141224 Msa0688870:CDS 55.0%
GGAATGGACCCAGTTGGGTA+TGG - chr4_4:55141210-55141229 Msa0688870:CDS 55.0%
TTTGGCACTAGACCCACCCT+TGG + chr4_4:55142196-55142215 None:intergenic 55.0%
ATAACAGTGGCACTCAGGGC+CGG - chr4_4:55142313-55142332 Msa0688870:intron 55.0%
AGGCATCTCATCTGCTGCAG+GGG - chr4_4:55142957-55142976 Msa0688870:CDS 55.0%
GTTGGATGGGATGGTCCCTC+AGG - chr4_4:55141188-55141207 Msa0688870:CDS 60.0%
TGGGATGGTCCCTCAGGGAA+TGG - chr4_4:55141194-55141213 Msa0688870:CDS 60.0%
CCAACTGGGTCCATTCCCTG+AGG + chr4_4:55141207-55141226 None:intergenic 60.0%
CCTCAGGGAATGGACCCAGT+TGG - chr4_4:55141204-55141223 Msa0688870:CDS 60.0%
! GTGGCCTTACACCCTTAGGC+CGG + chr4_4:55142335-55142354 None:intergenic 60.0%
!! ATCCATGCAGAGTGCCGCCA+GGG + chr4_4:55142501-55142520 None:intergenic 60.0%
TGCATCCATGCACAGTGCCG+CGG + chr4_4:55142525-55142544 None:intergenic 60.0%
! AGGGCCGGCCTAAGGGTGTA+AGG - chr4_4:55142328-55142347 Msa0688870:intron 65.0%
!! CATCCATGCAGAGTGCCGCC+AGG + chr4_4:55142502-55142521 None:intergenic 65.0%
GGCACTCTGCATGGATGCCG+CGG - chr4_4:55142505-55142524 Msa0688870:intron 65.0%
TGGCACTCAGGGCCGGCCTA+AGG - chr4_4:55142320-55142339 Msa0688870:intron 70.0%
GGCACTCAGGGCCGGCCTAA+GGG - chr4_4:55142321-55142340 Msa0688870:intron 70.0%
GGATGCCGCGGCACTGTGCA+TGG - chr4_4:55142517-55142536 Msa0688870:intron 70.0%
GGCCCTGGCGGCACTCTGCA+TGG - chr4_4:55142496-55142515 Msa0688870:intron 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 55140472 55143694 55140472 ID=Msa0688870;Name=Msa0688870
chr4_4 mRNA 55140472 55143694 55140472 ID=Msa0688870-mRNA-1;Parent=Msa0688870;Name=Msa0688870-mRNA-1;_AED=0.07;_eAED=0.07;_QI=357|1|1|1|0.75|0.6|5|566|153
chr4_4 exon 55140472 55141237 55140472 ID=Msa0688870-mRNA-1:exon:14555;Parent=Msa0688870-mRNA-1
chr4_4 exon 55142642 55142747 55142642 ID=Msa0688870-mRNA-1:exon:14554;Parent=Msa0688870-mRNA-1
chr4_4 exon 55142940 55143037 55142940 ID=Msa0688870-mRNA-1:exon:14553;Parent=Msa0688870-mRNA-1
chr4_4 exon 55143138 55143356 55143138 ID=Msa0688870-mRNA-1:exon:14552;Parent=Msa0688870-mRNA-1
chr4_4 exon 55143499 55143694 55143499 ID=Msa0688870-mRNA-1:exon:14551;Parent=Msa0688870-mRNA-1
chr4_4 five_prime_UTR 55143499 55143694 55143499 ID=Msa0688870-mRNA-1:five_prime_utr;Parent=Msa0688870-mRNA-1
chr4_4 five_prime_UTR 55143196 55143356 55143196 ID=Msa0688870-mRNA-1:five_prime_utr;Parent=Msa0688870-mRNA-1
chr4_4 CDS 55143138 55143195 55143138 ID=Msa0688870-mRNA-1:cds;Parent=Msa0688870-mRNA-1
chr4_4 CDS 55142940 55143037 55142940 ID=Msa0688870-mRNA-1:cds;Parent=Msa0688870-mRNA-1
chr4_4 CDS 55142642 55142747 55142642 ID=Msa0688870-mRNA-1:cds;Parent=Msa0688870-mRNA-1
chr4_4 CDS 55141038 55141237 55141038 ID=Msa0688870-mRNA-1:cds;Parent=Msa0688870-mRNA-1
chr4_4 three_prime_UTR 55140472 55141037 55140472 ID=Msa0688870-mRNA-1:three_prime_utr;Parent=Msa0688870-mRNA-1
Gene Sequence

>Msa0688870

ATGGCAACCACGTTTAAAGGAATTTGTAAGAGTTTGAAGTACATTACTCAGATGTTTGTTGTGAAGGAGCGAGAGATGGAAATTGGGTACCCGACGGATGTTAAACATGTGGCTCATGTTGGATGGGATGGTCCCTCAGGGAATGGACCCAGTTGGATGGATGATTTTAAAAAAGTTCCTGAATTTTCGACATCAATTGGTAACTTGGATGAAATTAGAGACTCTGATCCTATGGCTGTCACTTCATTATGGTCCTCCATAGAAAATCAACAATCATCAAACATTTACAAAGGCATCTCATCTGCTGCAGGGGATCCTCATATTGCAGAGAAGCCTAAGCAGAAAAAGGTTAAGTCAACTTCCTCTTCCTGGTCACCATCATCTTCATCAAGACGATCAAGAGCATCAAAATCAAAGGCTACCTTCAATGAGAGAGAAGCAATACCAATTGCTCTGGTGTAG
Protein sequence

>Msa0688870

MATTFKGICKSLKYITQMFVVKEREMEIGYPTDVKHVAHVGWDGPSGNGPSWMDDFKKVPEFSTSIGNLDEIRDSDPMAVTSLWSSIENQQSSNIYKGISSAAGDPHIAEKPKQKKVKSTSSSWSPSSSSRRSRASKSKATFNEREAIPIALV*