AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192330 MtrunA17_Chr2g0282511 87.654 324 22 5 1 321 1 309 6.97e-163 456
Msa0192330 MtrunA17_Chr4g0047001 45.078 386 120 18 14 321 13 384 7.41e-62 201
Msa0192330 MtrunA17_Chr4g0031411 43.931 173 69 5 41 210 72 219 5.13e-33 124
Msa0192330 MtrunA17_Chr2g0328021 45.113 133 71 2 10 142 29 159 3.99e-32 121
Msa0192330 MtrunA17_Chr4g0010151 69.231 91 25 2 41 128 79 169 4.61e-32 122
Msa0192330 MtrunA17_Chr8g0374251 71.429 70 20 0 39 108 63 132 1.39e-31 120
Msa0192330 MtrunA17_Chr8g0374111 67.647 68 22 0 40 107 25 92 1.00e-30 115
Msa0192330 MtrunA17_Chr4g0030051 74.242 66 17 0 31 96 28 93 1.42e-30 117
Msa0192330 MtrunA17_Chr1g0177251 48.800 125 59 2 41 163 9 130 1.56e-30 117
Msa0192330 MtrunA17_Chr1g0188851 55.670 97 32 2 15 100 3 99 2.24e-30 116
Msa0192330 MtrunA17_Chr2g0326001 71.642 67 19 0 30 96 26 92 4.31e-30 115
Msa0192330 MtrunA17_Chr7g0223311 68.571 70 22 0 37 106 70 139 4.42e-30 117
Msa0192330 MtrunA17_Chr8g0367161 67.647 68 22 0 35 102 23 90 1.08e-29 115
Msa0192330 MtrunA17_Chr8g0346551 55.435 92 30 1 31 122 11 91 1.17e-29 114
Msa0192330 MtrunA17_Chr8g0341671 72.727 66 16 1 40 105 16 79 1.83e-29 112
Msa0192330 MtrunA17_Chr3g0127201 71.014 69 17 1 34 102 17 82 2.23e-29 114
Msa0192330 MtrunA17_Chr7g0237921 80.702 57 11 0 40 96 72 128 4.16e-29 114
Msa0192330 MtrunA17_Chr2g0282051 64.865 74 25 1 27 100 29 101 4.33e-29 112
Msa0192330 MtrunA17_Chr3g0126201 62.338 77 29 0 29 105 36 112 1.90e-28 112
Msa0192330 MtrunA17_Chr5g0411951 79.630 54 11 0 41 94 40 93 2.84e-28 112
Msa0192330 MtrunA17_Chr2g0282441 75.410 61 15 0 35 95 16 76 4.64e-28 111
Msa0192330 MtrunA17_Chr3g0117601 72.131 61 17 0 34 94 51 111 8.81e-28 110
Msa0192330 MtrunA17_Chr2g0308011 86.000 50 7 0 43 92 36 85 1.44e-27 107
Msa0192330 MtrunA17_Chr4g0047761 70.270 74 21 1 28 101 56 128 1.55e-27 109
Msa0192330 MtrunA17_Chr4g0061761 81.132 53 10 0 42 94 38 90 1.62e-27 109
Msa0192330 MtrunA17_Chr6g0453321 54.321 81 33 1 20 96 108 188 1.59e-25 106
Msa0192330 MtrunA17_Chr3g0092111 62.121 66 25 0 38 103 130 195 2.10e-25 105
Msa0192330 MtrunA17_Chr7g0254161 49.495 99 49 1 35 133 92 189 9.18e-25 103
Msa0192330 MtrunA17_Chr2g0283811 59.677 62 25 0 34 95 37 98 1.49e-24 97.4
Msa0192330 MtrunA17_Chr7g0217581 57.895 76 25 1 30 98 211 286 2.18e-24 103
Msa0192330 MtrunA17_Chr4g0042481 53.333 75 35 0 34 108 112 186 2.53e-24 102
Msa0192330 MtrunA17_Chr5g0417561 43.077 130 73 1 13 141 88 217 2.71e-24 101
Msa0192330 MtrunA17_Chr8g0355261 48.810 84 39 1 34 117 108 187 3.38e-24 102
Msa0192330 MtrunA17_Chr6g0460721 40.000 130 77 1 24 153 69 197 6.61e-24 99.8
Msa0192330 MtrunA17_Chr6g0460731 50.685 73 36 0 24 96 76 148 4.56e-23 98.2
Msa0192330 MtrunA17_Chr5g0417571 50.000 82 39 1 15 96 111 190 6.43e-23 97.1
Msa0192330 MtrunA17_Chr5g0417551 50.000 84 42 0 13 96 96 179 2.60e-22 95.9
Msa0192330 MtrunA17_Chr5g0417601 50.633 79 37 1 21 99 119 195 7.09e-22 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192330 AT1G28310.1 41.503 306 130 12 44 321 27 311 1.90e-43 152
Msa0192330 AT1G28310.2 41.503 306 130 12 44 321 51 335 2.59e-43 152
Msa0192330 AT3G55370.1 53.333 105 49 0 4 108 36 140 3.01e-34 128
Msa0192330 AT3G55370.3 53.333 105 49 0 4 108 81 185 3.84e-34 128
Msa0192330 AT3G55370.2 53.333 105 49 0 4 108 36 140 5.20e-34 128
Msa0192330 AT1G51700.1 44.025 159 81 5 31 186 21 174 1.47e-33 122
Msa0192330 AT3G45610.1 41.463 164 75 5 41 202 37 181 5.56e-31 117
Msa0192330 AT5G60200.1 42.073 164 65 4 38 201 49 182 6.43e-31 117
Msa0192330 AT1G07640.2 60.000 85 26 1 28 104 53 137 7.58e-31 119
Msa0192330 AT1G07640.1 55.140 107 40 2 44 148 21 121 7.73e-31 117
Msa0192330 AT2G28810.2 81.034 58 11 0 38 95 71 128 8.98e-31 118
Msa0192330 AT1G07640.3 60.000 85 26 1 28 104 61 145 1.09e-30 119
Msa0192330 AT2G28810.1 81.034 58 11 0 38 95 88 145 1.19e-30 118
Msa0192330 AT3G50410.1 74.603 63 16 0 40 102 26 88 1.85e-30 116
Msa0192330 AT5G65590.1 60.000 85 34 0 19 103 16 100 1.89e-30 117
Msa0192330 AT2G37590.1 65.753 73 25 0 28 100 73 145 4.32e-30 117
Msa0192330 AT5G02460.1 87.037 54 7 0 43 96 94 147 2.11e-29 116
Msa0192330 AT1G21340.1 40.782 179 67 7 16 160 8 181 2.74e-29 113
Msa0192330 AT3G21270.1 78.947 57 12 0 44 100 29 85 3.73e-29 111
Msa0192330 AT2G28510.1 39.444 180 83 5 42 213 46 207 1.04e-28 112
Msa0192330 AT4G38000.1 71.875 64 18 0 34 97 31 94 2.45e-28 110
Msa0192330 AT5G66940.1 54.255 94 39 2 27 118 13 104 2.62e-28 109
Msa0192330 AT5G60850.1 73.770 61 16 0 40 100 47 107 6.97e-28 110
Msa0192330 AT5G62940.1 69.355 62 19 0 33 94 62 123 1.23e-26 108
Msa0192330 AT3G61850.2 66.667 66 19 1 37 102 55 117 1.29e-26 106
Msa0192330 AT3G61850.3 66.667 66 19 1 37 102 55 117 1.29e-26 106
Msa0192330 AT3G61850.5 66.667 66 19 1 37 102 55 117 1.72e-26 107
Msa0192330 AT3G61850.1 70.968 62 15 1 41 102 71 129 1.78e-26 106
Msa0192330 AT3G52440.1 74.194 62 14 1 46 105 27 88 2.29e-26 105
Msa0192330 AT3G61850.4 70.968 62 15 1 41 102 71 129 2.29e-26 106
Msa0192330 AT3G52440.2 74.194 62 14 1 46 105 46 107 3.20e-26 105
Msa0192330 AT4G00940.1 61.644 73 24 1 24 96 52 120 8.18e-26 104
Msa0192330 AT4G00940.3 61.644 73 24 1 24 96 52 120 8.18e-26 104
Msa0192330 AT4G00940.2 61.644 73 24 1 24 96 52 120 8.18e-26 104
Msa0192330 AT2G46590.1 71.930 57 16 0 35 91 59 115 8.77e-26 105
Msa0192330 AT2G46590.2 71.930 57 16 0 35 91 71 127 1.03e-25 105
Msa0192330 AT1G64620.1 69.492 59 18 0 34 92 39 97 1.76e-25 105
Msa0192330 AT3G47500.1 71.930 57 16 0 42 98 108 164 2.37e-25 105
Msa0192330 AT5G62430.1 62.903 62 23 0 38 99 48 109 7.09e-25 102
Msa0192330 AT5G39660.3 65.574 61 21 0 38 98 132 192 1.43e-24 103
Msa0192330 AT5G39660.1 65.574 61 21 0 38 98 132 192 1.43e-24 103
Msa0192330 AT5G39660.2 65.574 61 21 0 38 98 132 192 1.43e-24 103
Msa0192330 AT1G26790.1 47.573 103 52 2 20 122 88 188 3.04e-24 101
Msa0192330 AT1G47655.1 72.549 51 14 0 42 92 27 77 5.95e-24 97.8
Msa0192330 AT4G21050.1 76.000 50 12 0 46 95 26 75 1.43e-23 96.7
Msa0192330 AT1G29160.1 56.923 65 28 0 37 101 55 119 1.84e-23 95.5
Msa0192330 AT2G34140.1 56.923 65 28 0 37 101 51 115 2.12e-23 95.1
Msa0192330 AT4G24060.1 37.500 200 84 7 34 226 43 208 7.37e-23 97.4
Msa0192330 AT1G69570.1 56.716 67 29 0 38 104 126 192 9.10e-23 98.2
Msa0192330 AT4G21040.1 47.619 105 37 3 21 125 12 98 2.07e-22 94.0
Msa0192330 AT4G21080.1 42.857 105 47 2 21 125 12 103 1.82e-21 92.0
Msa0192330 AT4G21030.1 60.870 46 18 0 46 91 23 68 6.56e-13 67.0

Find 76 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCCTTAGTTCAAGTTTA+AGG 0.109986 2_1:+8095032 Msa0192330:three_prime_UTR
GGGCTTGGAATTTAACAATT+TGG 0.140338 2_1:+8094821 Msa0192330:CDS
GCAAAGCTTGCAAACGTTAT+TGG 0.143992 2_1:+8094262 Msa0192330:CDS
ACTCTTCAAGGATTCTATTT+TGG 0.149882 2_1:+8093800 Msa0192330:five_prime_UTR
AGGACTAATATTATGATTTA+AGG 0.162696 2_1:-8094454 None:intergenic
CACCAGCTTCTTCACTTTAC+TGG 0.188996 2_1:+8094913 Msa0192330:CDS
GGCTTGGAATTTAACAATTT+GGG 0.228469 2_1:+8094822 Msa0192330:CDS
GTTTAAGGTTATCTTTAATT+TGG 0.229380 2_1:+8095047 Msa0192330:three_prime_UTR
AACGATCAAGCTTCTAATAT+TGG 0.247824 2_1:+8094969 Msa0192330:CDS
TGTTGTCTGTATACTCTTCA+AGG 0.308452 2_1:+8093788 Msa0192330:five_prime_UTR
AATAGATGATGTTAAGATAA+TGG 0.329856 2_1:+8094116 Msa0192330:CDS
GCTTCAAGGGTTTCAAATAT+AGG 0.337429 2_1:-8093747 None:intergenic
TGTATAACCATTATGATCAT+TGG 0.338919 2_1:-8094637 None:intergenic
GGACAAGAGGTGGTACTCTT+AGG 0.348242 2_1:+8094283 Msa0192330:CDS
CAGATGAATACCATTGGATT+AGG 0.360977 2_1:+8094585 Msa0192330:CDS
ACAATCTAACTGAGGTTTGC+TGG 0.378078 2_1:-8095295 None:intergenic
TCTAGAATCTCAACAAGTTC+AGG 0.387293 2_1:+8094546 Msa0192330:CDS
CATCATCAGCTATTGGTGTT+TGG 0.393178 2_1:+8094946 Msa0192330:CDS
ATAACCTTAAACTTGAACTA+AGG 0.401805 2_1:-8095036 None:intergenic
GATCATAATGGTTATACAAA+TGG 0.418978 2_1:+8094642 Msa0192330:CDS
ATTATGATCATTGGCTTCGC+TGG 0.424556 2_1:-8094628 None:intergenic
CTTAGGAATGTTCCTGTTGG+TGG 0.434008 2_1:+8094300 Msa0192330:CDS
GCAGTTGGTCACTAGCTTCA+AGG 0.440834 2_1:-8093761 None:intergenic
ACAGCATCATCATCAGCTAT+TGG 0.454258 2_1:+8094939 Msa0192330:CDS
TTGGGGAATGGAAGTGAGAA+AGG 0.465690 2_1:+8094840 Msa0192330:CDS
CTTCTGTAACCACCACCAAC+AGG 0.474357 2_1:-8094312 None:intergenic
GAATTTAACAATTTGGGGAA+TGG 0.481892 2_1:+8094828 Msa0192330:CDS
TAAGGTTGAATTTGAAGAAG+TGG 0.481982 2_1:-8094436 None:intergenic
CAACATAAATTCATGAATGA+TGG 0.486443 2_1:+8094783 Msa0192330:CDS
CAAGCTTTGCAGAAATGTCT+TGG 0.496012 2_1:-8094249 None:intergenic
CTGATTAGTGGTTGGAGGGA+TGG 0.496794 2_1:-8093860 None:intergenic
CAGCCTCAGATGAATACCAT+TGG 0.500398 2_1:+8094579 Msa0192330:CDS
AAAGGAATGAAGAAAGATGA+AGG 0.500974 2_1:+8094858 Msa0192330:CDS
GAAGAAAGATGAAGGAGAGA+TGG 0.502257 2_1:+8094866 Msa0192330:CDS
TGAGCTAGAGGAGAGTACTT+TGG 0.503240 2_1:+8095245 Msa0192330:three_prime_UTR
GGCATAACTGATGCATTACT+TGG 0.503446 2_1:-8094735 None:intergenic
GAGATATTGAACCTTGAGAA+TGG 0.505952 2_1:-8094525 None:intergenic
CAGTTGGTCACTAGCTTCAA+GGG 0.509480 2_1:-8093760 None:intergenic
AACCTGTGGCCACTGATTAG+TGG 0.512866 2_1:-8093872 None:intergenic
GGCCACTGATTAGTGGTTGG+AGG 0.514127 2_1:-8093865 None:intergenic
AGAAGAATTAGTAGTATCAA+TGG 0.516034 2_1:-8094388 None:intergenic
AGGAATGTTCCTGTTGGTGG+TGG 0.516563 2_1:+8094303 Msa0192330:CDS
TTGCTGGATGCAGCTTACAT+AGG 0.517627 2_1:-8095279 None:intergenic
AGCGAAGCCAATGATCATAA+TGG 0.521310 2_1:+8094630 Msa0192330:CDS
ATGTGAATCTTCCATTCTCA+AGG 0.533312 2_1:+8094514 Msa0192330:CDS
TGAAGAAGTGGTGTTTGATG+TGG 0.539434 2_1:-8094424 None:intergenic
CTTGATAAACCATAAAACAT+AGG 0.540160 2_1:-8094474 None:intergenic
GGCTTCGCTGGACGTAAACC+CGG 0.542853 2_1:-8094616 None:intergenic
GCTTGGAATTTAACAATTTG+GGG 0.544684 2_1:+8094823 Msa0192330:CDS
ATTAGTAGTATCAATGGTGG+AGG 0.545112 2_1:-8094382 None:intergenic
AGAATTAGTAGTATCAATGG+TGG 0.550623 2_1:-8094385 None:intergenic
GCCACTGATTAGTGGTTGGA+GGG 0.566821 2_1:-8093864 None:intergenic
GAAAGGTAAAGGTGAGCTAG+AGG 0.570168 2_1:+8095233 Msa0192330:three_prime_UTR
TACAGACAACAAAGAGCAGT+TGG 0.576785 2_1:-8093776 None:intergenic
TGCAAACGTTATTGGACAAG+AGG 0.578125 2_1:+8094270 Msa0192330:CDS
ACTCTTAGGAATGTTCCTGT+TGG 0.582456 2_1:+8094297 Msa0192330:CDS
AACCACTAATCAGTGGCCAC+AGG 0.599050 2_1:+8093870 Msa0192330:CDS
TTCCAGTAAAGTGAAGAAGC+TGG 0.599918 2_1:-8094915 None:intergenic
GTGGTGTTTGATGTGGAACT+AGG 0.603982 2_1:-8094417 None:intergenic
TGAAGGAGAGATGGAACATG+TGG 0.611078 2_1:+8094875 Msa0192330:CDS
TTTCAAATATAGGAGAGTGA+GGG 0.620308 2_1:-8093737 None:intergenic
GTTTCAAATATAGGAGAGTG+AGG 0.621310 2_1:-8093738 None:intergenic
AGAGCAGAACTCAACAGAGA+AGG 0.622062 2_1:-8094756 None:intergenic
TTCAAATATAGGAGAGTGAG+GGG 0.622411 2_1:-8093736 None:intergenic
AATCCAATGGTATTCATCTG+AGG 0.625381 2_1:-8094582 None:intergenic
GGATGAAAAGCCTAATCCAA+TGG 0.636487 2_1:-8094595 None:intergenic
AAACGTTATTGGACAAGAGG+TGG 0.639210 2_1:+8094273 Msa0192330:CDS
CAGTAAAGTGAAGAAGCTGG+TGG 0.648703 2_1:-8094912 None:intergenic
TCCCTCCAACCACTAATCAG+TGG 0.668953 2_1:+8093863 Msa0192330:CDS
TAATAAGTAAAGCTAAACAA+AGG 0.672843 2_1:-8095072 None:intergenic
TAAAACTAACAATCTAACTG+AGG 0.677835 2_1:-8095303 None:intergenic
GAAGGAGAGATGGAACATGT+GGG 0.678274 2_1:+8094876 Msa0192330:CDS
TTAGTAGTATCAATGGTGGA+GGG 0.679047 2_1:-8094381 None:intergenic
TGTGGCCACTGATTAGTGGT+TGG 0.681850 2_1:-8093868 None:intergenic
GGAGAGATGGAACATGTGGG+TGG 0.710445 2_1:+8094879 Msa0192330:CDS
GTGTTTGATGAGTCACAACG+TGG 0.749573 2_1:-8094189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTGATATAAGAAAA+AGG - chr2_1:8094062-8094081 None:intergenic 10.0%
!! ATATAATTGATATAAGAAAA+AGG - chr2_1:8094062-8094081 None:intergenic 10.0%
!!! TTTGTTTAGTTTTGTATTTT+TGG + chr2_1:8095131-8095150 Msa0192330:three_prime_UTR 15.0%
!!! TTGTTTAGTTTTGTATTTTT+GGG + chr2_1:8095132-8095151 Msa0192330:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_1:8095177-8095196 Msa0192330:three_prime_UTR 15.0%
!!! TTTGTTTAGTTTTGTATTTT+TGG + chr2_1:8095131-8095150 Msa0192330:three_prime_UTR 15.0%
!!! TTGTTTAGTTTTGTATTTTT+GGG + chr2_1:8095132-8095151 Msa0192330:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_1:8095177-8095196 Msa0192330:three_prime_UTR 15.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_1:8094116-8094135 Msa0192330:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_1:8094457-8094476 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_1:8094465-8094484 Msa0192330:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_1:8095047-8095066 Msa0192330:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_1:8095075-8095094 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_1:8095191-8095210 Msa0192330:three_prime_UTR 20.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_1:8094116-8094135 Msa0192330:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_1:8094457-8094476 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_1:8094465-8094484 Msa0192330:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_1:8095047-8095066 Msa0192330:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_1:8095075-8095094 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_1:8095191-8095210 Msa0192330:three_prime_UTR 20.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_1:8093921-8093940 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_1:8093957-8093976 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_1:8093959-8093978 Msa0192330:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_1:8094028-8094047 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_1:8094029-8094048 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_1:8094126-8094145 Msa0192330:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_1:8094391-8094410 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_1:8094477-8094496 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_1:8094640-8094659 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_1:8094642-8094661 Msa0192330:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_1:8094783-8094802 Msa0192330:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_1:8095039-8095058 None:intergenic 25.0%
!!! TTAGTTTTGTATTTTTGGGT+TGG + chr2_1:8095136-8095155 Msa0192330:three_prime_UTR 25.0%
!!! TAGTTTTGTATTTTTGGGTT+GGG + chr2_1:8095137-8095156 Msa0192330:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_1:8095210-8095229 Msa0192330:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_1:8095211-8095230 Msa0192330:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_1:8095306-8095325 None:intergenic 25.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_1:8093921-8093940 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_1:8093957-8093976 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_1:8093959-8093978 Msa0192330:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_1:8094028-8094047 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_1:8094029-8094048 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_1:8094126-8094145 Msa0192330:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_1:8094391-8094410 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_1:8094477-8094496 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_1:8094640-8094659 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_1:8094642-8094661 Msa0192330:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_1:8094783-8094802 Msa0192330:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_1:8095039-8095058 None:intergenic 25.0%
!!! TTAGTTTTGTATTTTTGGGT+TGG + chr2_1:8095136-8095155 Msa0192330:three_prime_UTR 25.0%
!!! TAGTTTTGTATTTTTGGGTT+GGG + chr2_1:8095137-8095156 Msa0192330:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_1:8095210-8095229 Msa0192330:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_1:8095211-8095230 Msa0192330:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_1:8095306-8095325 None:intergenic 25.0%
! ACTCTTCAAGGATTCTATTT+TGG + chr2_1:8093800-8093819 Msa0192330:five_prime_UTR 30.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_1:8094388-8094407 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_1:8094439-8094458 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_1:8094822-8094841 Msa0192330:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_1:8094823-8094842 Msa0192330:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_1:8094828-8094847 Msa0192330:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_1:8094858-8094877 Msa0192330:CDS 30.0%
AACGATCAAGCTTCTAATAT+TGG + chr2_1:8094969-8094988 Msa0192330:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_1:8095032-8095051 Msa0192330:three_prime_UTR 30.0%
! ACTTGTTAGAAAAATGGGAA+AGG + chr2_1:8095216-8095235 Msa0192330:three_prime_UTR 30.0%
TAGAAAAATGGGAAAGGTAA+AGG + chr2_1:8095222-8095241 Msa0192330:three_prime_UTR 30.0%
! ACTCTTCAAGGATTCTATTT+TGG + chr2_1:8093800-8093819 Msa0192330:five_prime_UTR 30.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_1:8094388-8094407 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_1:8094439-8094458 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_1:8094822-8094841 Msa0192330:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_1:8094823-8094842 Msa0192330:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_1:8094828-8094847 Msa0192330:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_1:8094858-8094877 Msa0192330:CDS 30.0%
AACGATCAAGCTTCTAATAT+TGG + chr2_1:8094969-8094988 Msa0192330:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_1:8095032-8095051 Msa0192330:three_prime_UTR 30.0%
! ACTTGTTAGAAAAATGGGAA+AGG + chr2_1:8095216-8095235 Msa0192330:three_prime_UTR 30.0%
TAGAAAAATGGGAAAGGTAA+AGG + chr2_1:8095222-8095241 Msa0192330:three_prime_UTR 30.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_1:8093741-8093760 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_1:8093750-8093769 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_1:8093788-8093807 Msa0192330:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_1:8094384-8094403 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_1:8094385-8094404 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_1:8094514-8094533 Msa0192330:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_1:8094528-8094547 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_1:8094546-8094565 Msa0192330:CDS 35.0%
AATCCAATGGTATTCATCTG+AGG - chr2_1:8094585-8094604 None:intergenic 35.0%
CAGATGAATACCATTGGATT+AGG + chr2_1:8094585-8094604 Msa0192330:CDS 35.0%
!! ATTGGATTAGGCTTTTCATC+CGG + chr2_1:8094597-8094616 Msa0192330:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_1:8094821-8094840 Msa0192330:CDS 35.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_1:8093741-8093760 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_1:8093750-8093769 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_1:8093788-8093807 Msa0192330:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_1:8094384-8094403 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_1:8094385-8094404 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_1:8094514-8094533 Msa0192330:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_1:8094528-8094547 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_1:8094546-8094565 Msa0192330:CDS 35.0%
AATCCAATGGTATTCATCTG+AGG - chr2_1:8094585-8094604 None:intergenic 35.0%
CAGATGAATACCATTGGATT+AGG + chr2_1:8094585-8094604 Msa0192330:CDS 35.0%
!! ATTGGATTAGGCTTTTCATC+CGG + chr2_1:8094597-8094616 Msa0192330:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_1:8094821-8094840 Msa0192330:CDS 35.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_1:8093779-8093798 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_1:8094252-8094271 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_1:8094262-8094281 Msa0192330:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_1:8094270-8094289 Msa0192330:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_1:8094273-8094292 Msa0192330:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_1:8094297-8094316 Msa0192330:CDS 40.0%
TGAAGAAGTGGTGTTTGATG+TGG - chr2_1:8094427-8094446 None:intergenic 40.0%
GGATGAAAAGCCTAATCCAA+TGG - chr2_1:8094598-8094617 None:intergenic 40.0%
!! TTGGATTAGGCTTTTCATCC+GGG + chr2_1:8094598-8094617 Msa0192330:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_1:8094631-8094650 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_1:8094630-8094649 Msa0192330:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_1:8094738-8094757 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_1:8094800-8094819 Msa0192330:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_1:8094801-8094820 Msa0192330:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_1:8094866-8094885 Msa0192330:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_1:8094918-8094937 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_1:8094939-8094958 Msa0192330:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_1:8094946-8094965 Msa0192330:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_1:8095298-8095317 None:intergenic 40.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_1:8093779-8093798 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_1:8094252-8094271 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_1:8094262-8094281 Msa0192330:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_1:8094270-8094289 Msa0192330:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_1:8094273-8094292 Msa0192330:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_1:8094297-8094316 Msa0192330:CDS 40.0%
TGAAGAAGTGGTGTTTGATG+TGG - chr2_1:8094427-8094446 None:intergenic 40.0%
GGATGAAAAGCCTAATCCAA+TGG - chr2_1:8094598-8094617 None:intergenic 40.0%
!! TTGGATTAGGCTTTTCATCC+GGG + chr2_1:8094598-8094617 Msa0192330:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_1:8094631-8094650 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_1:8094630-8094649 Msa0192330:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_1:8094738-8094757 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_1:8094800-8094819 Msa0192330:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_1:8094801-8094820 Msa0192330:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_1:8094866-8094885 Msa0192330:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_1:8094918-8094937 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_1:8094939-8094958 Msa0192330:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_1:8094946-8094965 Msa0192330:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_1:8095298-8095317 None:intergenic 40.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_1:8093763-8093782 None:intergenic 45.0%
TGCATGGAGATAGAGAGAGA+GGG - chr2_1:8093905-8093924 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_1:8094192-8094211 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_1:8094300-8094319 Msa0192330:CDS 45.0%
GTGGTGTTTGATGTGGAACT+AGG - chr2_1:8094420-8094439 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_1:8094579-8094598 Msa0192330:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_1:8094759-8094778 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_1:8094806-8094825 Msa0192330:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_1:8094840-8094859 Msa0192330:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_1:8094875-8094894 Msa0192330:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_1:8094876-8094895 Msa0192330:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_1:8094915-8094934 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_1:8094913-8094932 Msa0192330:CDS 45.0%
GAAAGGTAAAGGTGAGCTAG+AGG + chr2_1:8095233-8095252 Msa0192330:three_prime_UTR 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_1:8095245-8095264 Msa0192330:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_1:8095282-8095301 None:intergenic 45.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_1:8093763-8093782 None:intergenic 45.0%
TGCATGGAGATAGAGAGAGA+GGG - chr2_1:8093905-8093924 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_1:8094192-8094211 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_1:8094300-8094319 Msa0192330:CDS 45.0%
GTGGTGTTTGATGTGGAACT+AGG - chr2_1:8094420-8094439 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_1:8094579-8094598 Msa0192330:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_1:8094759-8094778 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_1:8094806-8094825 Msa0192330:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_1:8094840-8094859 Msa0192330:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_1:8094875-8094894 Msa0192330:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_1:8094876-8094895 Msa0192330:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_1:8094915-8094934 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_1:8094913-8094932 Msa0192330:CDS 45.0%
GAAAGGTAAAGGTGAGCTAG+AGG + chr2_1:8095233-8095252 Msa0192330:three_prime_UTR 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_1:8095245-8095264 Msa0192330:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_1:8095282-8095301 None:intergenic 45.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_1:8093764-8093783 None:intergenic 50.0%
CTGATTAGTGGTTGGAGGGA+TGG - chr2_1:8093863-8093882 None:intergenic 50.0%
TCCCTCCAACCACTAATCAG+TGG + chr2_1:8093863-8093882 Msa0192330:CDS 50.0%
! GCCACTGATTAGTGGTTGGA+GGG - chr2_1:8093867-8093886 None:intergenic 50.0%
! TGTGGCCACTGATTAGTGGT+TGG - chr2_1:8093871-8093890 None:intergenic 50.0%
AACCACTAATCAGTGGCCAC+AGG + chr2_1:8093870-8093889 Msa0192330:CDS 50.0%
! AACCTGTGGCCACTGATTAG+TGG - chr2_1:8093875-8093894 None:intergenic 50.0%
GTGCATGGAGATAGAGAGAG+AGG - chr2_1:8093906-8093925 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_1:8094283-8094302 Msa0192330:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_1:8094303-8094322 Msa0192330:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_1:8094315-8094334 None:intergenic 50.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_1:8093764-8093783 None:intergenic 50.0%
CTGATTAGTGGTTGGAGGGA+TGG - chr2_1:8093863-8093882 None:intergenic 50.0%
TCCCTCCAACCACTAATCAG+TGG + chr2_1:8093863-8093882 Msa0192330:CDS 50.0%
! GCCACTGATTAGTGGTTGGA+GGG - chr2_1:8093867-8093886 None:intergenic 50.0%
! TGTGGCCACTGATTAGTGGT+TGG - chr2_1:8093871-8093890 None:intergenic 50.0%
AACCACTAATCAGTGGCCAC+AGG + chr2_1:8093870-8093889 Msa0192330:CDS 50.0%
! AACCTGTGGCCACTGATTAG+TGG - chr2_1:8093875-8093894 None:intergenic 50.0%
GTGCATGGAGATAGAGAGAG+AGG - chr2_1:8093906-8093925 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_1:8094283-8094302 Msa0192330:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_1:8094303-8094322 Msa0192330:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_1:8094315-8094334 None:intergenic 50.0%
! GGCCACTGATTAGTGGTTGG+AGG - chr2_1:8093868-8093887 None:intergenic 55.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_1:8093889-8093908 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_1:8094879-8094898 Msa0192330:CDS 55.0%
! GGCCACTGATTAGTGGTTGG+AGG - chr2_1:8093868-8093887 None:intergenic 55.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_1:8093889-8093908 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_1:8094879-8094898 Msa0192330:CDS 55.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_1:8094619-8094638 None:intergenic 60.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_1:8094619-8094638 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8093738 8095331 8093738 ID=Msa0192330;Name=Msa0192330
chr2_1 mRNA 8093738 8095331 8093738 ID=Msa0192330-mRNA-1;Parent=Msa0192330;Name=Msa0192330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=94|1|1|1|1|1|2|315|321
chr2_1 exon 8093738 8093891 8093738 ID=Msa0192330-mRNA-1:exon:3752;Parent=Msa0192330-mRNA-1
chr2_1 exon 8094111 8095331 8094111 ID=Msa0192330-mRNA-1:exon:3753;Parent=Msa0192330-mRNA-1
chr2_1 five_prime_UTR 8093738 8093831 8093738 ID=Msa0192330-mRNA-1:five_prime_utr;Parent=Msa0192330-mRNA-1
chr2_1 CDS 8093832 8093891 8093832 ID=Msa0192330-mRNA-1:cds;Parent=Msa0192330-mRNA-1
chr2_1 CDS 8094111 8095016 8094111 ID=Msa0192330-mRNA-1:cds;Parent=Msa0192330-mRNA-1
chr2_1 three_prime_UTR 8095017 8095331 8095017 ID=Msa0192330-mRNA-1:three_prime_utr;Parent=Msa0192330-mRNA-1
Gene Sequence

>Msa0192330

ATGTTATCTAACTATGAGAAAATGCTTGCCATCCCTCCAACCACTAATCAGTGGCCACAGAATCAAATAGATGATGTTAAGATAATGGAAAAACAAGGTCAAGAACTTCAGCAGCAGCAGCAGCAAGCTCTCAAGTGTCCACGTTGTGACTCATCAAACACAAAGTTTTGTTACTACAACAACTACAGTTTGTCACAACCAAGACATTTCTGCAAAGCTTGCAAACGTTATTGGACAAGAGGTGGTACTCTTAGGAATGTTCCTGTTGGTGGTGGTTACAGAAGAAACAACTCAAACAAACGTAGTAACAATACTACTCTCATCAAAAGACCCTCCACCATTGATACTACTAATTCTTCTTCAACTCCTAGTTCCACATCAAACACCACTTCTTCAAATTCAACCTTAAATCATAATATTAGTCCTATGTTTTATGGTTTATCAAGTAGTAATAACTCTTGTGATGTGAATCTTCCATTCTCAAGGTTCAATATCTCTAGAATCTCAACAAGTTCAGGTTATGATTTTCAGCCTCAGATGAATACCATTGGATTAGGCTTTTCATCCGGGTTTACGTCCAGCGAAGCCAATGATCATAATGGTTATACAAATGGATTTACTTCAAATAATAATAATAACACTCTTGTCTCAAGATGTGATTCAATCTTTAGCTCTTCTTCTGCACCAAGTAATGCATCAGTTATGCCTTCTCTGTTGAGTTCTGCTCTGCTGCAACATAAATTCATGAATGATGGTAGCAACACTTTTCAGGGCTTGGAATTTAACAATTTGGGGAATGGAAGTGAGAAAGGAATGAAGAAAGATGAAGGAGAGATGGAACATGTGGGTGGTTTGTATGATCCACCAGCTTCTTCACTTTACTGGAATACAGCATCATCATCAGCTATTGGTGTTTGGAACGATCAAGCTTCTAATATTGGTTCATCAGTTACTTCTTTGATCTAG
Protein sequence

>Msa0192330

MLSNYEKMLAIPPTTNQWPQNQIDDVKIMEKQGQELQQQQQQALKCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGYRRNNSNKRSNNTTLIKRPSTIDTTNSSSTPSSTSNTTSSNSTLNHNISPMFYGLSSSNNSCDVNLPFSRFNISRISTSSGYDFQPQMNTIGLGFSSGFTSSEANDHNGYTNGFTSNNNNNTLVSRCDSIFSSSSAPSNASVMPSLLSSALLQHKFMNDGSNTFQGLEFNNLGNGSEKGMKKDEGEMEHVGGLYDPPASSLYWNTASSSAIGVWNDQASNIGSSVTSLI*