AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0197190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197190 MtrunA17_Chr2g0289391 97.216 431 12 0 1 431 1 431 0.0 869
Msa0197190 MtrunA17_Chr3g0099911 56.532 421 179 4 13 430 29 448 2.07e-167 478
Msa0197190 MtrunA17_Chr1g0147671 33.094 417 259 5 14 429 46 443 2.76e-69 226
Msa0197190 MtrunA17_Chr8g0377091 31.144 411 266 6 24 429 50 448 7.49e-65 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197190 AT3G20300.1 54.907 428 183 6 12 431 26 451 3.18e-162 465
Msa0197190 AT4G22270.2 55.416 397 175 2 36 431 40 435 2.36e-145 421
Msa0197190 AT4G22270.1 55.190 395 175 2 36 429 40 433 8.23e-144 417
Msa0197190 AT1G50630.1 52.103 428 191 5 16 431 27 452 4.09e-137 401
Msa0197190 AT4G03820.1 50.877 399 191 4 35 429 36 433 2.12e-129 380
Msa0197190 AT4G03820.2 50.750 400 192 4 35 430 36 434 2.21e-129 381
Msa0197190 AT4G03820.3 50.877 399 191 4 35 429 85 482 5.06e-129 381
Msa0197190 AT4G03820.4 50.750 400 192 4 35 430 85 483 9.86e-129 381
Msa0197190 AT1G50630.2 49.487 390 183 5 16 393 27 414 2.84e-119 354
Msa0197190 AT1G67570.1 30.361 415 274 6 21 429 46 451 3.24e-62 208
Msa0197190 AT2G21080.1 29.157 415 254 7 23 429 29 411 1.34e-60 203
Msa0197190 AT1G67570.2 28.421 380 257 6 21 394 46 416 2.60e-48 171

Find 108 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCGGGAATCACAGTTTA+TGG 0.195361 2_1:+14937666 Msa0197190:CDS
AAGATTGTCAAAGGAGTTTA+TGG 0.208064 2_1:-14937378 None:intergenic
TTCAATTGGCTCTTTGTCTT+TGG 0.230096 2_1:+14937730 Msa0197190:CDS
TCTCACCATAATATGGTATC+TGG 0.237823 2_1:-14936468 None:intergenic
TATGTGTTGATTTGGTTAAT+TGG 0.245532 2_1:+14935172 Msa0197190:five_prime_UTR
TACCTAAGGCCTGTCTCTTA+TGG 0.263903 2_1:-14937550 None:intergenic
TTGTTTGAATCTCATTGTTC+TGG 0.272128 2_1:+14937863 Msa0197190:three_prime_UTR
CATGAAGCTCATCTTGCTTT+GGG 0.292329 2_1:+14936384 Msa0197190:CDS
TCCAAATGGGATCGTAAATA+TGG 0.300939 2_1:+14935540 Msa0197190:CDS
GCAATGAACAAAGAGAATTC+TGG 0.321057 2_1:+14935267 Msa0197190:CDS
GCGAATAGTGTTGAGACTAT+TGG 0.329613 2_1:+14937922 Msa0197190:three_prime_UTR
CCATGAAGCTCATCTTGCTT+TGG 0.336140 2_1:+14936383 Msa0197190:CDS
TGCGTCCTCTTTCGACTCAT+TGG 0.342923 2_1:+14936568 Msa0197190:CDS
TTCAAATGTCTCTAACTATC+AGG 0.344175 2_1:-14935053 None:intergenic
AACTCCTTTGACAATCTTGA+TGG 0.350116 2_1:+14937383 Msa0197190:CDS
TCCTCAAGGGTGGAGAGCTT+GGG 0.352090 2_1:+14936806 Msa0197190:CDS
ACCATATTTACGATCCCATT+TGG 0.352766 2_1:-14935541 None:intergenic
CATCCAGTCTTTGTATTTGA+AGG 0.355933 2_1:-14936594 None:intergenic
GTCTCCATCACCTTGGTTAG+TGG 0.357131 2_1:+14937266 Msa0197190:CDS
ACATACATCTCACCATAATA+TGG 0.357699 2_1:-14936475 None:intergenic
TGTTTGAATTGGCATCATTT+TGG 0.360106 2_1:-14937513 None:intergenic
GCATGTTTGTTAAATTGTTA+TGG 0.362484 2_1:+14938153 Msa0197190:three_prime_UTR
TGTCTCTAACTATCAGGTTC+TGG 0.369554 2_1:-14935047 None:intergenic
CGAATAGTGTTGAGACTATT+GGG 0.375524 2_1:+14937923 Msa0197190:three_prime_UTR
GACACATTGACATATGAAAC+AGG 0.387480 2_1:-14936413 None:intergenic
TATAAAACTTCAATTTCCTT+TGG 0.389501 2_1:+14938191 Msa0197190:three_prime_UTR
ATCCTCAAGGGTGGAGAGCT+TGG 0.390467 2_1:+14936805 Msa0197190:CDS
CCAAAGCAAGATGAGCTTCA+TGG 0.392242 2_1:-14936383 None:intergenic
CAGCTTTGCATAGATTTGAT+TGG 0.394117 2_1:-14935353 None:intergenic
GTATCCCAGATACCATATTA+TGG 0.401465 2_1:+14936463 Msa0197190:CDS
GATTCATGCTTGACAGAACT+TGG 0.416415 2_1:+14937688 Msa0197190:CDS
AGTGTTTGTTGCTATGTATA+TGG 0.425468 2_1:+14938045 Msa0197190:three_prime_UTR
GAAATAGTATGTGTTTGAAT+TGG 0.433805 2_1:-14937524 None:intergenic
GTAGGTTCCTTCTTATTCTC+AGG 0.434596 2_1:-14936672 None:intergenic
GCACATTGGAGCTATGTTCA+TGG 0.435576 2_1:+14936515 Msa0197190:CDS
AATATTATAATTAATTATCC+AGG 0.438851 2_1:+14938121 Msa0197190:three_prime_UTR
AGAGGATCCCCAACTAATAT+CGG 0.447276 2_1:-14937450 None:intergenic
TATGGCTGCCGATATTAGTT+GGG 0.458915 2_1:+14937442 Msa0197190:CDS
GATATTCCCCGCTGAGTAGT+AGG 0.459814 2_1:-14937969 None:intergenic
CTATGGCTGCCGATATTAGT+TGG 0.462105 2_1:+14937441 Msa0197190:CDS
GTCAATGTGTCTACAAGATA+TGG 0.465509 2_1:+14936425 Msa0197190:CDS
ACAGAAAGAGAAATCTGAAC+AGG 0.469099 2_1:-14935489 None:intergenic
TATGCAAAGCTGGTATATCT+TGG 0.470355 2_1:+14935364 Msa0197190:CDS
GGATCCTCTGAAGATGAATC+AGG 0.471723 2_1:+14937464 Msa0197190:CDS
GATTCAGAGCATATCTAAGA+TGG 0.476865 2_1:+14935319 Msa0197190:CDS
CCTGCGTGTCATCCGATATG+AGG 0.480554 2_1:-14937991 None:intergenic
CTACCTTCAAATACAAAGAC+TGG 0.486038 2_1:+14936591 Msa0197190:CDS
AGCGGGGAATATCCTCATAT+CGG 0.486364 2_1:+14937979 Msa0197190:three_prime_UTR
TGCGTCTCCTACTACTCAGC+GGG 0.489897 2_1:+14937962 Msa0197190:three_prime_UTR
TATTTCATTCCATAAGAGAC+AGG 0.519592 2_1:+14937541 Msa0197190:CDS
CAATCAAATCTATGCAAAGC+TGG 0.522455 2_1:+14935354 Msa0197190:CDS
AAGATATGGTGGTACGTCTC+CGG 0.522936 2_1:+14936439 Msa0197190:CDS
CAAACATGCAAACAAATGCC+TGG 0.523755 2_1:-14938139 None:intergenic
AGAGGTAGGAACAATCTTGT+TGG 0.530764 2_1:+14936645 Msa0197190:CDS
AATTCTGGAATATATGATGA+TGG 0.534661 2_1:+14935282 Msa0197190:CDS
CTCACCATAATATGGTATCT+GGG 0.537109 2_1:-14936467 None:intergenic
GACTATGGAAGAAGAAAGAG+AGG 0.537851 2_1:+14935233 Msa0197190:exon
TGGCTATGCTCAACAAATGA+AGG 0.538863 2_1:+14935620 Msa0197190:CDS
ATCTCCTGATTCATCTTCAG+AGG 0.539906 2_1:-14937468 None:intergenic
ATGATGATGGAAAAGAATTG+AGG 0.546124 2_1:+14935295 Msa0197190:CDS
TGGCTACTGAACAAGACTGT+TGG 0.555327 2_1:+14937750 Msa0197190:CDS
GCAGTTAGTCTCCATCACCT+TGG 0.557148 2_1:+14937259 Msa0197190:intron
TTCCATAAGAGACAGGCCTT+AGG 0.557758 2_1:+14937548 Msa0197190:CDS
GATAGCTTCAGCACAAGCTA+TGG 0.558099 2_1:+14937424 Msa0197190:CDS
TCACAGGTCTTGCTGCTAAG+TGG 0.563319 2_1:+14937342 Msa0197190:CDS
ATTCAGAGCATATCTAAGAT+GGG 0.565496 2_1:+14935320 Msa0197190:CDS
ATCTGAACAGGAACATGGTA+CGG 0.568447 2_1:-14935477 None:intergenic
TATCGGATGACACGCAGGAC+AGG 0.569696 2_1:+14937996 Msa0197190:three_prime_UTR
CAGCATCATTCTGTGCACAT+TGG 0.570513 2_1:+14936501 Msa0197190:CDS
AGATGAATCAGGAGATGAAG+AGG 0.571260 2_1:+14937475 Msa0197190:CDS
CCCCAAGCTCTCCACCCTTG+AGG 0.573937 2_1:-14936807 None:intergenic
AAGTCCACTAACCAAGGTGA+TGG 0.576355 2_1:-14937270 None:intergenic
CTCACCATCAAGATTGTCAA+AGG 0.578128 2_1:-14937387 None:intergenic
CCTCAAGGGTGGAGAGCTTG+GGG 0.578144 2_1:+14936807 Msa0197190:CDS
ATGAAGCTCATCTTGCTTTG+GGG 0.581113 2_1:+14936385 Msa0197190:CDS
GTTGGTATCTAGAGACAGAA+AGG 0.583421 2_1:+14937768 Msa0197190:exon
GGTAGCCAATGAGTCGAAAG+AGG 0.586577 2_1:-14936573 None:intergenic
GCTGAAGCTATCAGTGCTGT+AGG 0.587919 2_1:-14937413 None:intergenic
AGAAAACAATGCTGATATTG+CGG 0.591077 2_1:-14935088 None:intergenic
ATATGGTATCTGGGATACTC+CGG 0.594382 2_1:-14936458 None:intergenic
TATATGGAGAACAATAGAGC+GGG 0.622842 2_1:+14937651 Msa0197190:CDS
TGGAGCACCTGAGAATAAGA+AGG 0.623856 2_1:+14936665 Msa0197190:CDS
CTTAGCAGCAAGACCTGTGA+GGG 0.624292 2_1:-14937339 None:intergenic
CCTCATATCGGATGACACGC+AGG 0.630077 2_1:+14937991 Msa0197190:three_prime_UTR
CATAAAGCACAATCCCTCAC+AGG 0.632924 2_1:+14937326 Msa0197190:CDS
GAGAAATCTGAACAGGAACA+TGG 0.633500 2_1:-14935482 None:intergenic
TGCTGATGTTGATATCCTCA+AGG 0.633605 2_1:+14936792 Msa0197190:CDS
GCATACCAAGAAGAAAATTG+AGG 0.639938 2_1:-14936548 None:intergenic
ACATTGCACAAGGTAGGACT+AGG 0.642514 2_1:-14937812 None:intergenic
AGGATATCAACATCAGCATG+TGG 0.645352 2_1:-14936787 None:intergenic
TATGTAAAGTCCACTAACCA+AGG 0.647878 2_1:-14937276 None:intergenic
ATGGCTGCCGATATTAGTTG+GGG 0.653210 2_1:+14937443 Msa0197190:CDS
AAGGTAGGACTAGGACTAGG+AGG 0.659803 2_1:-14937803 None:intergenic
CACAAGGTAGGACTAGGACT+AGG 0.662506 2_1:-14937806 None:intergenic
AAATGAAAGCTCGAAAACGA+TGG 0.672388 2_1:-14936705 None:intergenic
TACTGTACATTGCACAAGGT+AGG 0.682293 2_1:-14937818 None:intergenic
CTGCGTCTCCTACTACTCAG+CGG 0.687212 2_1:+14937961 Msa0197190:three_prime_UTR
GATGTTGATATCCTCAAGGG+TGG 0.689625 2_1:+14936796 Msa0197190:CDS
ACTTAGCAGCAAGACCTGTG+AGG 0.692562 2_1:-14937340 None:intergenic
GCTGATGTTGATATCCTCAA+GGG 0.701097 2_1:+14936793 Msa0197190:CDS
GATGTACTGTACATTGCACA+AGG 0.704916 2_1:-14937822 None:intergenic
ATATATGGAGAACAATAGAG+CGG 0.709937 2_1:+14937650 Msa0197190:CDS
ACACATTGACATATGAAACA+GGG 0.716196 2_1:-14936412 None:intergenic
AATGTGTCTACAAGATATGG+TGG 0.717610 2_1:+14936428 Msa0197190:CDS
ATATCTAAGATGGGTTTACG+TGG 0.723343 2_1:+14935329 Msa0197190:CDS
GCGTCTCCTACTACTCAGCG+GGG 0.736864 2_1:+14937963 Msa0197190:three_prime_UTR
AACGATGGCTAATGACACGT+AGG 0.739267 2_1:-14936690 None:intergenic
GAGAGTCTCAAGATTCAACG+TGG 0.795417 2_1:+14935600 Msa0197190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGATATTATTCAATTTAA+GGG + chr2_1:14937115-14937134 Msa0197190:intron 10.0%
!! AATATTATAATTAATTATCC+AGG + chr2_1:14938121-14938140 Msa0197190:three_prime_UTR 10.0%
!! ACATAAAAAGTTTGAATAAT+TGG - chr2_1:14935157-14935176 None:intergenic 15.0%
!!! GTTTTTTTATTTCTTCTATT+TGG + chr2_1:14935648-14935667 Msa0197190:intron 15.0%
!!! TAATAATTGTATGGATTATA+TGG + chr2_1:14935879-14935898 Msa0197190:intron 15.0%
!!! TAGTTATAACTAATTTTCAT+TGG + chr2_1:14936200-14936219 Msa0197190:intron 15.0%
!!! AGTTATAACTAATTTTCATT+GGG + chr2_1:14936201-14936220 Msa0197190:intron 15.0%
!!! TTACTAAATATATCTTTTAC+TGG - chr2_1:14936984-14937003 None:intergenic 15.0%
!! GATTGATATTATTCAATTTA+AGG + chr2_1:14937114-14937133 Msa0197190:intron 15.0%
!! TAAAAAGTTTGAATAATTGG+TGG - chr2_1:14935154-14935173 None:intergenic 20.0%
!!! AAACTTTTTATGTGTTGATT+TGG + chr2_1:14935164-14935183 Msa0197190:five_prime_UTR 20.0%
!!! ACATAAATTTTTGAAGACTA+TGG + chr2_1:14935218-14935237 Msa0197190:exon 20.0%
!! TCTATTTGATGAATAGTTAT+AGG + chr2_1:14935807-14935826 Msa0197190:intron 20.0%
!!! ATCCTAATCTTTTTCAATTT+GGG - chr2_1:14935860-14935879 None:intergenic 20.0%
!!! TATCCTAATCTTTTTCAATT+TGG - chr2_1:14935861-14935880 None:intergenic 20.0%
!! AGATTAGGATAATAATTGTA+TGG + chr2_1:14935870-14935889 Msa0197190:intron 20.0%
!!! TATATGGTTAATTTGATTGA+AGG + chr2_1:14935895-14935914 Msa0197190:intron 20.0%
!!! ATATGGTTAATTTGATTGAA+GGG + chr2_1:14935896-14935915 Msa0197190:intron 20.0%
!! TCTTGTGTAAAAATAAGATA+AGG + chr2_1:14935972-14935991 Msa0197190:intron 20.0%
!!! TTTTTTTGTTGAACAAGATT+CGG - chr2_1:14936056-14936075 None:intergenic 20.0%
!! ATCTTGTTCAACAAAAAAAT+AGG + chr2_1:14936057-14936076 Msa0197190:intron 20.0%
!! TGATGCAATAAAAAACATAA+TGG - chr2_1:14936274-14936293 None:intergenic 20.0%
!! ATTTCACATTTCTTAAATGA+AGG - chr2_1:14936849-14936868 None:intergenic 20.0%
!!! TAAATATATCTTTTACTGGA+AGG - chr2_1:14936980-14936999 None:intergenic 20.0%
!!! AATTTGCTATTTCACAAATT+TGG + chr2_1:14937141-14937160 Msa0197190:intron 20.0%
!! CACTAAAAATAGATTGTAAT+AGG + chr2_1:14937218-14937237 Msa0197190:intron 20.0%
!! AATAAATTTCCATTTACCTA+AGG - chr2_1:14937567-14937586 None:intergenic 20.0%
!! TATAAAACTTCAATTTCCTT+TGG + chr2_1:14938191-14938210 Msa0197190:three_prime_UTR 20.0%
!!! TATGTGTTGATTTGGTTAAT+TGG + chr2_1:14935172-14935191 Msa0197190:five_prime_UTR 25.0%
!!! AATTCTGGAATATATGATGA+TGG + chr2_1:14935282-14935301 Msa0197190:CDS 25.0%
!!! TTTTATTAGTCTCTCCAAAT+GGG + chr2_1:14935527-14935546 Msa0197190:CDS 25.0%
!!! ATCGTAAATATGGTTTTAGT+AGG + chr2_1:14935550-14935569 Msa0197190:CDS 25.0%
! AAGCATGCATCAAAATTAAA+CGG - chr2_1:14935715-14935734 None:intergenic 25.0%
!! ATGAACAATGAATTTTGTCA+TGG + chr2_1:14935748-14935767 Msa0197190:intron 25.0%
!! TATGGTTAATTTGATTGAAG+GGG + chr2_1:14935897-14935916 Msa0197190:intron 25.0%
!!! TTTTTTGTTGAACAAGATTC+GGG - chr2_1:14936055-14936074 None:intergenic 25.0%
!! ATAACTAATTTTCATTGGGT+TGG + chr2_1:14936205-14936224 Msa0197190:intron 25.0%
! CTTATGTAACTATTTGACAA+TGG - chr2_1:14936242-14936261 None:intergenic 25.0%
! CATTGTCAAATAGTTACATA+AGG + chr2_1:14936240-14936259 Msa0197190:intron 25.0%
! GATTTCTATTTCAGTTAGAA+TGG - chr2_1:14936311-14936330 None:intergenic 25.0%
!!! TCTGTTTCTTTTTGAAAAAC+AGG - chr2_1:14936628-14936647 None:intergenic 25.0%
!!! TGTTTTTCAAAAAGAAACAG+AGG + chr2_1:14936627-14936646 Msa0197190:CDS 25.0%
!!! ATTTACTCATTTCTAGTGTT+TGG - chr2_1:14936916-14936935 None:intergenic 25.0%
! TCTAAACCTATGAAAACTTA+CGG + chr2_1:14937011-14937030 Msa0197190:intron 25.0%
! GACAAACATAACATTATTAG+TGG + chr2_1:14937064-14937083 Msa0197190:intron 25.0%
! TTTAGACCTTGAAATTGTAA+GGG - chr2_1:14937184-14937203 None:intergenic 25.0%
!! TTTTAGACCTTGAAATTGTA+AGG - chr2_1:14937185-14937204 None:intergenic 25.0%
! GAAATAGTATGTGTTTGAAT+TGG - chr2_1:14937527-14937546 None:intergenic 25.0%
!! TTTTATTGCAGTGACATATA+TGG + chr2_1:14937635-14937654 Msa0197190:intron 25.0%
!! CATAAACTTTTTATTCGTAG+CGG - chr2_1:14938077-14938096 None:intergenic 25.0%
!! GCATGTTTGTTAAATTGTTA+TGG + chr2_1:14938153-14938172 Msa0197190:three_prime_UTR 25.0%
TTCAAATGTCTCTAACTATC+AGG - chr2_1:14935056-14935075 None:intergenic 30.0%
AGAAAACAATGCTGATATTG+CGG - chr2_1:14935091-14935110 None:intergenic 30.0%
!! ATGATGATGGAAAAGAATTG+AGG + chr2_1:14935295-14935314 Msa0197190:CDS 30.0%
! ATTCAGAGCATATCTAAGAT+GGG + chr2_1:14935320-14935339 Msa0197190:CDS 30.0%
AGTAAAAAGTGAGACAGAAT+AGG - chr2_1:14935420-14935439 None:intergenic 30.0%
!! CTTTTATTAGTCTCTCCAAA+TGG + chr2_1:14935526-14935545 Msa0197190:CDS 30.0%
AAGACAAACACAATTACACA+AGG - chr2_1:14935777-14935796 None:intergenic 30.0%
TGCCCAAATTGAAAAAGATT+AGG + chr2_1:14935855-14935874 Msa0197190:intron 30.0%
! TCAATAGTGTGTTCTCTTTT+TGG - chr2_1:14936166-14936185 None:intergenic 30.0%
ACACATTGACATATGAAACA+GGG - chr2_1:14936415-14936434 None:intergenic 30.0%
ACATACATCTCACCATAATA+TGG - chr2_1:14936478-14936497 None:intergenic 30.0%
! TAGAACCTCAATTTTCTTCT+TGG + chr2_1:14936543-14936562 Msa0197190:CDS 30.0%
TTTCAAAAAGAAACAGAGGT+AGG + chr2_1:14936631-14936650 Msa0197190:CDS 30.0%
!! TTGCTATTTCACAAATTTGG+AGG + chr2_1:14937144-14937163 Msa0197190:intron 30.0%
AAGATTGTCAAAGGAGTTTA+TGG - chr2_1:14937381-14937400 None:intergenic 30.0%
! TGTTTGAATTGGCATCATTT+TGG - chr2_1:14937516-14937535 None:intergenic 30.0%
TATTTCATTCCATAAGAGAC+AGG + chr2_1:14937541-14937560 Msa0197190:CDS 30.0%
ATATATGGAGAACAATAGAG+CGG + chr2_1:14937650-14937669 Msa0197190:CDS 30.0%
!!! CTCCATTTTTGGTATTCAAT+TGG + chr2_1:14937716-14937735 Msa0197190:CDS 30.0%
AGCCAATTGAATACCAAAAA+TGG - chr2_1:14937721-14937740 None:intergenic 30.0%
ACAAAAAGATGACTGTTCAA+AGG - chr2_1:14937847-14937866 None:intergenic 30.0%
TTGTTTGAATCTCATTGTTC+TGG + chr2_1:14937863-14937882 Msa0197190:three_prime_UTR 30.0%
! AGTGTTTGTTGCTATGTATA+TGG + chr2_1:14938045-14938064 Msa0197190:three_prime_UTR 30.0%
!!! AGAAAGAGAGGCTAGTTTTT+TGG + chr2_1:14935245-14935264 Msa0197190:CDS 35.0%
GCAATGAACAAAGAGAATTC+TGG + chr2_1:14935267-14935286 Msa0197190:CDS 35.0%
GATTCAGAGCATATCTAAGA+TGG + chr2_1:14935319-14935338 Msa0197190:CDS 35.0%
! ATATCTAAGATGGGTTTACG+TGG + chr2_1:14935329-14935348 Msa0197190:CDS 35.0%
CAGCTTTGCATAGATTTGAT+TGG - chr2_1:14935356-14935375 None:intergenic 35.0%
CAATCAAATCTATGCAAAGC+TGG + chr2_1:14935354-14935373 Msa0197190:CDS 35.0%
TATGCAAAGCTGGTATATCT+TGG + chr2_1:14935364-14935383 Msa0197190:CDS 35.0%
! TTGGTCTGTGTTTTTCACTT+TGG + chr2_1:14935383-14935402 Msa0197190:CDS 35.0%
ACAGAAAGAGAAATCTGAAC+AGG - chr2_1:14935492-14935511 None:intergenic 35.0%
TCCAAATGGGATCGTAAATA+TGG + chr2_1:14935540-14935559 Msa0197190:CDS 35.0%
ACCATATTTACGATCCCATT+TGG - chr2_1:14935544-14935563 None:intergenic 35.0%
GCTGCATACAATACATCAAA+TGG + chr2_1:14935994-14936013 Msa0197190:intron 35.0%
GCATACAATACATCAAATGG+TGG + chr2_1:14935997-14936016 Msa0197190:intron 35.0%
! CACAAGGAGAGTTATGTTTT+AGG + chr2_1:14936101-14936120 Msa0197190:intron 35.0%
TGTGCAGATTATGCAACTAA+GGG - chr2_1:14936143-14936162 None:intergenic 35.0%
GACACATTGACATATGAAAC+AGG - chr2_1:14936416-14936435 None:intergenic 35.0%
GTCAATGTGTCTACAAGATA+TGG + chr2_1:14936425-14936444 Msa0197190:CDS 35.0%
AATGTGTCTACAAGATATGG+TGG + chr2_1:14936428-14936447 Msa0197190:CDS 35.0%
GTATCCCAGATACCATATTA+TGG + chr2_1:14936463-14936482 Msa0197190:CDS 35.0%
CTCACCATAATATGGTATCT+GGG - chr2_1:14936470-14936489 None:intergenic 35.0%
TCTCACCATAATATGGTATC+TGG - chr2_1:14936471-14936490 None:intergenic 35.0%
GCATACCAAGAAGAAAATTG+AGG - chr2_1:14936551-14936570 None:intergenic 35.0%
CTACCTTCAAATACAAAGAC+TGG + chr2_1:14936591-14936610 Msa0197190:CDS 35.0%
CATCCAGTCTTTGTATTTGA+AGG - chr2_1:14936597-14936616 None:intergenic 35.0%
AAATGAAAGCTCGAAAACGA+TGG - chr2_1:14936708-14936727 None:intergenic 35.0%
! CATTTTGTCTTCTCTGCTTT+TGG + chr2_1:14936723-14936742 Msa0197190:CDS 35.0%
CCATAAGTAGAAAAATGAGC+TGG - chr2_1:14936759-14936778 None:intergenic 35.0%
!! CCAGCTCATTTTTCTACTTA+TGG + chr2_1:14936756-14936775 Msa0197190:CDS 35.0%
AGCAAAAGAACGCAAAAGAA+CGG - chr2_1:14936876-14936895 None:intergenic 35.0%
! CTCAATCCGTAAGTTTTCAT+AGG - chr2_1:14937020-14937039 None:intergenic 35.0%
TATGTAAAGTCCACTAACCA+AGG - chr2_1:14937279-14937298 None:intergenic 35.0%
AACTCCTTTGACAATCTTGA+TGG + chr2_1:14937383-14937402 Msa0197190:CDS 35.0%
TATATGGAGAACAATAGAGC+GGG + chr2_1:14937651-14937670 Msa0197190:CDS 35.0%
! TTCAATTGGCTCTTTGTCTT+TGG + chr2_1:14937730-14937749 Msa0197190:CDS 35.0%
! CGAATAGTGTTGAGACTATT+GGG + chr2_1:14937923-14937942 Msa0197190:three_prime_UTR 35.0%
TGTCTCTAACTATCAGGTTC+TGG - chr2_1:14935050-14935069 None:intergenic 40.0%
GACTATGGAAGAAGAAAGAG+AGG + chr2_1:14935233-14935252 Msa0197190:exon 40.0%
ATCTGAACAGGAACATGGTA+CGG - chr2_1:14935480-14935499 None:intergenic 40.0%
GAGAAATCTGAACAGGAACA+TGG - chr2_1:14935485-14935504 None:intergenic 40.0%
TGGCTATGCTCAACAAATGA+AGG + chr2_1:14935620-14935639 Msa0197190:CDS 40.0%
TGGGCAAAAACGTTGAATTG+TGG - chr2_1:14935841-14935860 None:intergenic 40.0%
TGCAACGGTAAAGTTGTTGT+CGG + chr2_1:14935928-14935947 Msa0197190:intron 40.0%
GTGTGCAGATTATGCAACTA+AGG - chr2_1:14936144-14936163 None:intergenic 40.0%
CATGAAGCTCATCTTGCTTT+GGG + chr2_1:14936384-14936403 Msa0197190:CDS 40.0%
ATGAAGCTCATCTTGCTTTG+GGG + chr2_1:14936385-14936404 Msa0197190:CDS 40.0%
! ATATGGTATCTGGGATACTC+CGG - chr2_1:14936461-14936480 None:intergenic 40.0%
AGAGGTAGGAACAATCTTGT+TGG + chr2_1:14936645-14936664 Msa0197190:CDS 40.0%
GTAGGTTCCTTCTTATTCTC+AGG - chr2_1:14936675-14936694 None:intergenic 40.0%
AGGATATCAACATCAGCATG+TGG - chr2_1:14936790-14936809 None:intergenic 40.0%
TGCTGATGTTGATATCCTCA+AGG + chr2_1:14936792-14936811 Msa0197190:CDS 40.0%
GCTGATGTTGATATCCTCAA+GGG + chr2_1:14936793-14936812 Msa0197190:CDS 40.0%
GACAGACCCTTACAATTTCA+AGG + chr2_1:14937175-14937194 Msa0197190:intron 40.0%
CTCACCATCAAGATTGTCAA+AGG - chr2_1:14937390-14937409 None:intergenic 40.0%
TATGGCTGCCGATATTAGTT+GGG + chr2_1:14937442-14937461 Msa0197190:CDS 40.0%
AGAGGATCCCCAACTAATAT+CGG - chr2_1:14937453-14937472 None:intergenic 40.0%
ATCTCCTGATTCATCTTCAG+AGG - chr2_1:14937471-14937490 None:intergenic 40.0%
AGATGAATCAGGAGATGAAG+AGG + chr2_1:14937475-14937494 Msa0197190:CDS 40.0%
GATTCATGCTTGACAGAACT+TGG + chr2_1:14937688-14937707 Msa0197190:CDS 40.0%
! ACTTGGCTTCACTCCATTTT+TGG + chr2_1:14937705-14937724 Msa0197190:CDS 40.0%
GTTGGTATCTAGAGACAGAA+AGG + chr2_1:14937768-14937787 Msa0197190:exon 40.0%
TACTGTACATTGCACAAGGT+AGG - chr2_1:14937821-14937840 None:intergenic 40.0%
GATGTACTGTACATTGCACA+AGG - chr2_1:14937825-14937844 None:intergenic 40.0%
! GCGAATAGTGTTGAGACTAT+TGG + chr2_1:14937922-14937941 Msa0197190:three_prime_UTR 40.0%
CAAACATGCAAACAAATGCC+TGG - chr2_1:14938142-14938161 None:intergenic 40.0%
GAGAGTCTCAAGATTCAACG+TGG + chr2_1:14935600-14935619 Msa0197190:CDS 45.0%
GCAACGGTAAAGTTGTTGTC+GGG + chr2_1:14935929-14935948 Msa0197190:intron 45.0%
TTGTTGTCGGGTTCAAGTCT+TGG + chr2_1:14935941-14935960 Msa0197190:intron 45.0%
CCAAAGCAAGATGAGCTTCA+TGG - chr2_1:14936386-14936405 None:intergenic 45.0%
CCATGAAGCTCATCTTGCTT+TGG + chr2_1:14936383-14936402 Msa0197190:CDS 45.0%
AAGATATGGTGGTACGTCTC+CGG + chr2_1:14936439-14936458 Msa0197190:CDS 45.0%
CAGCATCATTCTGTGCACAT+TGG + chr2_1:14936501-14936520 Msa0197190:CDS 45.0%
GCACATTGGAGCTATGTTCA+TGG + chr2_1:14936515-14936534 Msa0197190:CDS 45.0%
! TGGAGCACCTGAGAATAAGA+AGG + chr2_1:14936665-14936684 Msa0197190:CDS 45.0%
AACGATGGCTAATGACACGT+AGG - chr2_1:14936693-14936712 None:intergenic 45.0%
!! GTCTTCTCTGCTTTTGGTGA+CGG + chr2_1:14936729-14936748 Msa0197190:CDS 45.0%
GATGTTGATATCCTCAAGGG+TGG + chr2_1:14936796-14936815 Msa0197190:CDS 45.0%
AAGTCCACTAACCAAGGTGA+TGG - chr2_1:14937273-14937292 None:intergenic 45.0%
CATAAAGCACAATCCCTCAC+AGG + chr2_1:14937326-14937345 Msa0197190:CDS 45.0%
GATAGCTTCAGCACAAGCTA+TGG + chr2_1:14937424-14937443 Msa0197190:CDS 45.0%
CTATGGCTGCCGATATTAGT+TGG + chr2_1:14937441-14937460 Msa0197190:CDS 45.0%
ATGGCTGCCGATATTAGTTG+GGG + chr2_1:14937443-14937462 Msa0197190:CDS 45.0%
! GGATCCTCTGAAGATGAATC+AGG + chr2_1:14937464-14937483 Msa0197190:CDS 45.0%
TTCCATAAGAGACAGGCCTT+AGG + chr2_1:14937548-14937567 Msa0197190:CDS 45.0%
TACCTAAGGCCTGTCTCTTA+TGG - chr2_1:14937553-14937572 None:intergenic 45.0%
AGAGACAGGCCTTAGGTAAA+TGG + chr2_1:14937555-14937574 Msa0197190:intron 45.0%
AGAGCGGGAATCACAGTTTA+TGG + chr2_1:14937666-14937685 Msa0197190:CDS 45.0%
TGGCTACTGAACAAGACTGT+TGG + chr2_1:14937750-14937769 Msa0197190:CDS 45.0%
ACATTGCACAAGGTAGGACT+AGG - chr2_1:14937815-14937834 None:intergenic 45.0%
AGCGGGGAATATCCTCATAT+CGG + chr2_1:14937979-14937998 Msa0197190:three_prime_UTR 45.0%
GAAGGGGAGTCTTGATGCAA+CGG + chr2_1:14935913-14935932 Msa0197190:intron 50.0%
!! CCTTGTGCTGCTTCTGTTTC+TGG - chr2_1:14936088-14936107 None:intergenic 50.0%
CCAGAAACAGAAGCAGCACA+AGG + chr2_1:14936085-14936104 Msa0197190:intron 50.0%
TGCGTCCTCTTTCGACTCAT+TGG + chr2_1:14936568-14936587 Msa0197190:CDS 50.0%
GGTAGCCAATGAGTCGAAAG+AGG - chr2_1:14936576-14936595 None:intergenic 50.0%
! GCAGTTAGTCTCCATCACCT+TGG + chr2_1:14937259-14937278 Msa0197190:intron 50.0%
GTCTCCATCACCTTGGTTAG+TGG + chr2_1:14937266-14937285 Msa0197190:CDS 50.0%
CTTAGCAGCAAGACCTGTGA+GGG - chr2_1:14937342-14937361 None:intergenic 50.0%
ACTTAGCAGCAAGACCTGTG+AGG - chr2_1:14937343-14937362 None:intergenic 50.0%
TCACAGGTCTTGCTGCTAAG+TGG + chr2_1:14937342-14937361 Msa0197190:CDS 50.0%
GCTGAAGCTATCAGTGCTGT+AGG - chr2_1:14937416-14937435 None:intergenic 50.0%
AAGGTAGGACTAGGACTAGG+AGG - chr2_1:14937806-14937825 None:intergenic 50.0%
CACAAGGTAGGACTAGGACT+AGG - chr2_1:14937809-14937828 None:intergenic 50.0%
GATATTCCCCGCTGAGTAGT+AGG - chr2_1:14937972-14937991 None:intergenic 50.0%
GTGGAACCTCTTCTCAGACC+CGG + chr2_1:14936016-14936035 Msa0197190:intron 55.0%
TGGAACCTCTTCTCAGACCC+GGG + chr2_1:14936017-14936036 Msa0197190:intron 55.0%
ATCCTCAAGGGTGGAGAGCT+TGG + chr2_1:14936805-14936824 Msa0197190:CDS 55.0%
TCCTCAAGGGTGGAGAGCTT+GGG + chr2_1:14936806-14936825 Msa0197190:CDS 55.0%
CTGCGTCTCCTACTACTCAG+CGG + chr2_1:14937961-14937980 Msa0197190:three_prime_UTR 55.0%
TGCGTCTCCTACTACTCAGC+GGG + chr2_1:14937962-14937981 Msa0197190:three_prime_UTR 55.0%
CCTGCGTGTCATCCGATATG+AGG - chr2_1:14937994-14938013 None:intergenic 55.0%
CCTCATATCGGATGACACGC+AGG + chr2_1:14937991-14938010 Msa0197190:three_prime_UTR 55.0%
TATCGGATGACACGCAGGAC+AGG + chr2_1:14937996-14938015 Msa0197190:three_prime_UTR 55.0%
AAGCTCCCGGGTCTGAGAAG+AGG - chr2_1:14936025-14936044 None:intergenic 60.0%
CTTCTCAGACCCGGGAGCTT+CGG + chr2_1:14936025-14936044 Msa0197190:intron 60.0%
TTCGGGTGATCCGAAGCTCC+CGG - chr2_1:14936038-14936057 None:intergenic 60.0%
CCTCAAGGGTGGAGAGCTTG+GGG + chr2_1:14936807-14936826 Msa0197190:CDS 60.0%
GCGTCTCCTACTACTCAGCG+GGG + chr2_1:14937963-14937982 Msa0197190:three_prime_UTR 60.0%
TCGGGTGATCCGAAGCTCCC+GGG - chr2_1:14936037-14936056 None:intergenic 65.0%
CCCCAAGCTCTCCACCCTTG+AGG - chr2_1:14936810-14936829 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 14935008 14938215 14935008 ID=Msa0197190;Name=Msa0197190
chr2_1 mRNA 14935008 14938215 14935008 ID=Msa0197190-mRNA-1;Parent=Msa0197190;Name=Msa0197190-mRNA-1;_AED=0.03;_eAED=0.03;_QI=229|1|1|1|1|1|4|436|431
chr2_1 exon 14935008 14935641 14935008 ID=Msa0197190-mRNA-1:exon:6340;Parent=Msa0197190-mRNA-1
chr2_1 exon 14936379 14936828 14936379 ID=Msa0197190-mRNA-1:exon:6341;Parent=Msa0197190-mRNA-1
chr2_1 exon 14937263 14937569 14937263 ID=Msa0197190-mRNA-1:exon:6342;Parent=Msa0197190-mRNA-1
chr2_1 exon 14937646 14938215 14937646 ID=Msa0197190-mRNA-1:exon:6343;Parent=Msa0197190-mRNA-1
chr2_1 five_prime_UTR 14935008 14935236 14935008 ID=Msa0197190-mRNA-1:five_prime_utr;Parent=Msa0197190-mRNA-1
chr2_1 CDS 14935237 14935641 14935237 ID=Msa0197190-mRNA-1:cds;Parent=Msa0197190-mRNA-1
chr2_1 CDS 14936379 14936828 14936379 ID=Msa0197190-mRNA-1:cds;Parent=Msa0197190-mRNA-1
chr2_1 CDS 14937263 14937569 14937263 ID=Msa0197190-mRNA-1:cds;Parent=Msa0197190-mRNA-1
chr2_1 CDS 14937646 14937779 14937646 ID=Msa0197190-mRNA-1:cds;Parent=Msa0197190-mRNA-1
chr2_1 three_prime_UTR 14937780 14938215 14937780 ID=Msa0197190-mRNA-1:three_prime_utr;Parent=Msa0197190-mRNA-1
Gene Sequence

>Msa0197190

ATGGAAGAAGAAAGAGAGGCTAGTTTTTTGGCAATGAACAAAGAGAATTCTGGAATATATGATGATGGAAAAGAATTGAGGAGATTCAGAGCATATCTAAGATGGGTTTACGTGGACCAATCAAATCTATGCAAAGCTGGTATATCTTGGTCTGTGTTTTTCACTTTGGCTTACGTTGTACCTATTCTGTCTCACTTTTTACTTGATTGTTCGACAACGTGTGATGCTGATCATCGTAGACCGTACCATGTTCCTGTTCAGATTTCTCTTTCTGTCTTTGCAACTTTGTCTTTTATTAGTCTCTCCAAATGGGATCGTAAATATGGTTTTAGTAGGTTTCTTTTTCTTGATAAAGTTAGTGATGAGAGTCTCAAGATTCAACGTGGCTATGCTCAACAAATGAAGAGAACCATGAAGCTCATCTTGCTTTGGGGACTTCCCTGTTTCATATGTCAATGTGTCTACAAGATATGGTGGTACGTCTCCGGAGTATCCCAGATACCATATTATGGTGAGATGTATGTGAGCAGCATCATTCTGTGCACATTGGAGCTATGTTCATGGTTTTATAGAACCTCAATTTTCTTCTTGGTATGCGTCCTCTTTCGACTCATTGGCTACCTTCAAATACAAAGACTGGATGAATTTGCTCCTGTTTTTCAAAAAGAAACAGAGGTAGGAACAATCTTGTTGGAGCACCTGAGAATAAGAAGGAACCTACGTGTCATTAGCCATCGTTTTCGAGCTTTCATTTTGTCTTCTCTGCTTTTGGTGACGGCAAGCCAGCTCATTTTTCTACTTATGGTTATAAAACCACATGCTGATGTTGATATCCTCAAGGGTGGAGAGCTTGGGTTAGTCTCCATCACCTTGGTTAGTGGACTTTACATACTCTTACGAAGCGCAACGAAGATCACACATAAAGCACAATCCCTCACAGGTCTTGCTGCTAAGTGGCATATATGTGCTACCATAAACTCCTTTGACAATCTTGATGGTGAGACACCTACAGCACTGATAGCTTCAGCACAAGCTATGGCTGCCGATATTAGTTGGGGATCCTCTGAAGATGAATCAGGAGATGAAGAGGATGAGTTGAATAACACCAAAATGATGCCAATTCAAACACATACTATTTCATTCCATAAGAGACAGGCCTTAGTGACATATATGGAGAACAATAGAGCGGGAATCACAGTTTATGGATTCATGCTTGACAGAACTTGGCTTCACTCCATTTTTGGTATTCAATTGGCTCTTTGTCTTTGGCTACTGAACAAGACTGTTGGTATCTAG
Protein sequence

>Msa0197190

MEEEREASFLAMNKENSGIYDDGKELRRFRAYLRWVYVDQSNLCKAGISWSVFFTLAYVVPILSHFLLDCSTTCDADHRRPYHVPVQISLSVFATLSFISLSKWDRKYGFSRFLFLDKVSDESLKIQRGYAQQMKRTMKLILLWGLPCFICQCVYKIWWYVSGVSQIPYYGEMYVSSIILCTLELCSWFYRTSIFFLVCVLFRLIGYLQIQRLDEFAPVFQKETEVGTILLEHLRIRRNLRVISHRFRAFILSSLLLVTASQLIFLLMVIKPHADVDILKGGELGLVSITLVSGLYILLRSATKITHKAQSLTGLAAKWHICATINSFDNLDGETPTALIASAQAMAADISWGSSEDESGDEEDELNNTKMMPIQTHTISFHKRQALVTYMENNRAGITVYGFMLDRTWLHSIFGIQLALCLWLLNKTVGI*