AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0308780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308780 MtrunA17_Chr2g0289391 97.680 431 10 0 1 431 1 431 0.0 874
Msa0308780 MtrunA17_Chr3g0099911 56.770 421 178 4 13 430 29 448 9.22e-169 481
Msa0308780 MtrunA17_Chr1g0147671 33.251 406 254 4 25 429 54 443 9.87e-69 224
Msa0308780 MtrunA17_Chr8g0377091 31.144 411 266 6 24 429 50 448 9.26e-65 214
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0308780 AT3G20300.1 55.140 428 182 6 12 431 26 451 1.22e-163 468
Msa0308780 AT4G22270.2 55.416 397 175 2 36 431 40 435 2.17e-145 421
Msa0308780 AT4G22270.1 55.190 395 175 2 36 429 40 433 8.42e-144 417
Msa0308780 AT1G50630.1 52.336 428 190 5 16 431 27 452 2.19e-138 404
Msa0308780 AT4G03820.1 50.877 399 191 4 35 429 36 433 1.29e-129 381
Msa0308780 AT4G03820.2 50.750 400 192 4 35 430 36 434 2.36e-129 381
Msa0308780 AT4G03820.3 50.877 399 191 4 35 429 85 482 6.57e-129 381
Msa0308780 AT4G03820.4 50.750 400 192 4 35 430 85 483 8.94e-129 381
Msa0308780 AT1G50630.2 49.744 390 182 5 16 393 27 414 1.49e-120 358
Msa0308780 AT1G67570.1 30.097 412 273 6 24 429 49 451 7.07e-62 207
Msa0308780 AT2G21080.1 29.157 415 254 7 23 429 29 411 1.30e-60 203
Msa0308780 AT1G67570.2 28.382 377 255 6 24 394 49 416 6.48e-48 170

Find 108 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCGGGAATCACAGTTTA+TGG 0.195361 2_4:+3218191 Msa0308780:CDS
AAGATTGTCAAAGGAGTTTA+TGG 0.208064 2_4:-3217903 None:intergenic
TTCAATTGGCTCTTTGTCTT+TGG 0.230096 2_4:+3218255 Msa0308780:CDS
TCTCACCATAATATGGTATC+TGG 0.237823 2_4:-3216993 None:intergenic
TATGTGTTGATTTGGTTAAT+TGG 0.245532 2_4:+3215697 Msa0308780:five_prime_UTR
TACCTAAGGCCTGTCTCTTA+TGG 0.263903 2_4:-3218075 None:intergenic
TTGTTTGAATCTCATTGTTC+TGG 0.272128 2_4:+3218388 Msa0308780:three_prime_UTR
CATGAAGCTCATCTTGCTTT+GGG 0.292329 2_4:+3216909 Msa0308780:CDS
TCCAAATGGGATCGTAAATA+TGG 0.300939 2_4:+3216065 Msa0308780:CDS
GCGAATAGTGTTGAGACTAT+TGG 0.329613 2_4:+3218447 Msa0308780:three_prime_UTR
GCAATGAACAAAGAAAATTC+TGG 0.330000 2_4:+3215792 Msa0308780:CDS
CCATGAAGCTCATCTTGCTT+TGG 0.336140 2_4:+3216908 Msa0308780:CDS
TGCGTCCTCTTTCGACTCAT+TGG 0.342923 2_4:+3217093 Msa0308780:CDS
TTCAAATGTCTCTAACTATC+AGG 0.344175 2_4:-3215578 None:intergenic
AACTCCTTTGACAATCTTGA+TGG 0.350116 2_4:+3217908 Msa0308780:CDS
TCCTCAAGGGTGGAGAGCTT+GGG 0.352090 2_4:+3217331 Msa0308780:CDS
ACCATATTTACGATCCCATT+TGG 0.352766 2_4:-3216066 None:intergenic
CATCCAGTCTTTGTATTTGA+AGG 0.355933 2_4:-3217119 None:intergenic
GTCTCCATCACCTTGGTTAG+TGG 0.357131 2_4:+3217791 Msa0308780:CDS
ACATACATCTCACCATAATA+TGG 0.357699 2_4:-3217000 None:intergenic
TGTTTGAATTGGCATCATTT+TGG 0.360106 2_4:-3218038 None:intergenic
GCATGTTTGTTAAATTGTTA+TGG 0.362484 2_4:+3218678 Msa0308780:three_prime_UTR
TGTCTCTAACTATCAGGTTC+TGG 0.369554 2_4:-3215572 None:intergenic
CAGCTTTGCATAAATTTGAT+TGG 0.374305 2_4:-3215878 None:intergenic
CGAATAGTGTTGAGACTATT+GGG 0.375524 2_4:+3218448 Msa0308780:three_prime_UTR
GACACATTGACATATGAAAC+AGG 0.387480 2_4:-3216938 None:intergenic
TATAAAACTTCAATTTCCTT+TGG 0.389501 2_4:+3218716 Msa0308780:three_prime_UTR
ATCCTCAAGGGTGGAGAGCT+TGG 0.390467 2_4:+3217330 Msa0308780:CDS
CCAAAGCAAGATGAGCTTCA+TGG 0.392242 2_4:-3216908 None:intergenic
GTATCCCAGATACCATATTA+TGG 0.401465 2_4:+3216988 Msa0308780:CDS
TATGCAAAGCTGGTATATCT+TGG 0.416276 2_4:+3215889 Msa0308780:CDS
GATTCATGCTTGACAGAACT+TGG 0.416415 2_4:+3218213 Msa0308780:CDS
AGTGTTTGTTGCTATGTATA+TGG 0.425468 2_4:+3218570 Msa0308780:three_prime_UTR
GAAATAGTATGTGTTTGAAT+TGG 0.433805 2_4:-3218049 None:intergenic
GTAGGTTCCTTCTTATTCTC+AGG 0.434596 2_4:-3217197 None:intergenic
GCACATTGGAGCTATGTTCA+TGG 0.435576 2_4:+3217040 Msa0308780:CDS
AATATTATAATTAATTATCC+AGG 0.438851 2_4:+3218646 Msa0308780:three_prime_UTR
AGAGGATCCCCAACTAATAT+CGG 0.447276 2_4:-3217975 None:intergenic
TATGGCTGCCGATATTAGTT+GGG 0.458915 2_4:+3217967 Msa0308780:CDS
GATATTCCCCGCTGAGTAGT+AGG 0.459814 2_4:-3218494 None:intergenic
CTATGGCTGCCGATATTAGT+TGG 0.462105 2_4:+3217966 Msa0308780:CDS
CAATCAAATTTATGCAAAGC+TGG 0.463784 2_4:+3215879 Msa0308780:CDS
GTCAATGTGTCTACAAGATA+TGG 0.465509 2_4:+3216950 Msa0308780:CDS
ACAGAAAGAGAAATCTGAAC+AGG 0.469099 2_4:-3216014 None:intergenic
GGATCCTCTGAAGATGAATC+AGG 0.471723 2_4:+3217989 Msa0308780:CDS
GATTCAGAGCATATCTAAGA+TGG 0.476865 2_4:+3215844 Msa0308780:CDS
CCTGCGTGTCATCCGATATG+AGG 0.480554 2_4:-3218516 None:intergenic
CTACCTTCAAATACAAAGAC+TGG 0.486038 2_4:+3217116 Msa0308780:CDS
AGCGGGGAATATCCTCATAT+CGG 0.486364 2_4:+3218504 Msa0308780:three_prime_UTR
TGCGTCTCCTACTACTCAGC+GGG 0.489897 2_4:+3218487 Msa0308780:three_prime_UTR
GGCTATGGAAGAAGAAAGAG+AGG 0.490472 2_4:+3215758 Msa0308780:exon
TATTTCATTCCATAAGAGAC+AGG 0.519592 2_4:+3218066 Msa0308780:CDS
AAGATATGGTGGTACGTCTC+CGG 0.522936 2_4:+3216964 Msa0308780:CDS
CAAACATGCAAACAAATGCC+TGG 0.523755 2_4:-3218664 None:intergenic
AGAGGTAGGAACAATCTTGT+TGG 0.530764 2_4:+3217170 Msa0308780:CDS
ATCATGATGGAAAAGAATTG+AGG 0.534961 2_4:+3215820 Msa0308780:CDS
CTCACCATAATATGGTATCT+GGG 0.537109 2_4:-3216992 None:intergenic
TGGCTATGCTCAACAAATGA+AGG 0.538863 2_4:+3216145 Msa0308780:CDS
ATCTCCTGATTCATCTTCAG+AGG 0.539906 2_4:-3217993 None:intergenic
TGGCTACTGAACAAGACTGT+TGG 0.555327 2_4:+3218275 Msa0308780:CDS
GCAGTTAGTCTCCATCACCT+TGG 0.557148 2_4:+3217784 Msa0308780:intron
TTCCATAAGAGACAGGCCTT+AGG 0.557758 2_4:+3218073 Msa0308780:CDS
GATAGCTTCAGCACAAGCTA+TGG 0.558099 2_4:+3217949 Msa0308780:CDS
TCACAGGTCTTGCTGCTAAG+TGG 0.563319 2_4:+3217867 Msa0308780:CDS
ATTCAGAGCATATCTAAGAT+GGG 0.565496 2_4:+3215845 Msa0308780:CDS
AATTCTGGAATATATCATGA+TGG 0.568112 2_4:+3215807 Msa0308780:CDS
ATCTGAACAGGAACATGGTA+CGG 0.568447 2_4:-3216002 None:intergenic
TATCGGATGACACGCAGGAC+AGG 0.569696 2_4:+3218521 Msa0308780:three_prime_UTR
CAGCATCATTCTGTGCACAT+TGG 0.570513 2_4:+3217026 Msa0308780:CDS
AGATGAATCAGGAGATGAAG+AGG 0.571260 2_4:+3218000 Msa0308780:CDS
CCCCAAGCTCTCCACCCTTG+AGG 0.573937 2_4:-3217332 None:intergenic
AAGTCCACTAACCAAGGTGA+TGG 0.576355 2_4:-3217795 None:intergenic
CTCACCATCAAGATTGTCAA+AGG 0.578128 2_4:-3217912 None:intergenic
CCTCAAGGGTGGAGAGCTTG+GGG 0.578144 2_4:+3217332 Msa0308780:CDS
ATGAAGCTCATCTTGCTTTG+GGG 0.581113 2_4:+3216910 Msa0308780:CDS
GTTGGTATCTAGAGACAGAA+AGG 0.583421 2_4:+3218293 Msa0308780:exon
GGTAGCCAATGAGTCGAAAG+AGG 0.586577 2_4:-3217098 None:intergenic
GCTGAAGCTATCAGTGCTGT+AGG 0.587919 2_4:-3217938 None:intergenic
AGAAAACAATGCTGATATTG+CGG 0.591077 2_4:-3215613 None:intergenic
ATATGGTATCTGGGATACTC+CGG 0.594382 2_4:-3216983 None:intergenic
TATATGGAGAACAATAGAGC+GGG 0.622842 2_4:+3218176 Msa0308780:CDS
TGGAGCACCTGAGAATAAGA+AGG 0.623856 2_4:+3217190 Msa0308780:CDS
CTTAGCAGCAAGACCTGTGA+GGG 0.624292 2_4:-3217864 None:intergenic
CCTCATATCGGATGACACGC+AGG 0.630077 2_4:+3218516 Msa0308780:three_prime_UTR
CATAAAGCACAATCCCTCAC+AGG 0.632924 2_4:+3217851 Msa0308780:CDS
GAGAAATCTGAACAGGAACA+TGG 0.633500 2_4:-3216007 None:intergenic
TGCTGATGTTGATATCCTCA+AGG 0.633605 2_4:+3217317 Msa0308780:CDS
GCATACCAAGAAGAAAATTG+AGG 0.639938 2_4:-3217073 None:intergenic
ACATTGCACAAGGTAGGACT+AGG 0.642514 2_4:-3218337 None:intergenic
AGGATATCAACATCAGCATG+TGG 0.645352 2_4:-3217312 None:intergenic
TATGTAAAGTCCACTAACCA+AGG 0.647878 2_4:-3217801 None:intergenic
ATGGCTGCCGATATTAGTTG+GGG 0.653210 2_4:+3217968 Msa0308780:CDS
AAGGTAGGACTAGGACTAGG+AGG 0.659803 2_4:-3218328 None:intergenic
CACAAGGTAGGACTAGGACT+AGG 0.662506 2_4:-3218331 None:intergenic
AAATGAAAGCTCGAAAACGA+TGG 0.672388 2_4:-3217230 None:intergenic
TACTGTACATTGCACAAGGT+AGG 0.682293 2_4:-3218343 None:intergenic
CTGCGTCTCCTACTACTCAG+CGG 0.687212 2_4:+3218486 Msa0308780:three_prime_UTR
GATGTTGATATCCTCAAGGG+TGG 0.689625 2_4:+3217321 Msa0308780:CDS
ACTTAGCAGCAAGACCTGTG+AGG 0.692562 2_4:-3217865 None:intergenic
GCTGATGTTGATATCCTCAA+GGG 0.701097 2_4:+3217318 Msa0308780:CDS
GATGTACTGTACATTGCACA+AGG 0.704916 2_4:-3218347 None:intergenic
ATATATGGAGAACAATAGAG+CGG 0.709937 2_4:+3218175 Msa0308780:CDS
ACACATTGACATATGAAACA+GGG 0.716196 2_4:-3216937 None:intergenic
AATGTGTCTACAAGATATGG+TGG 0.717610 2_4:+3216953 Msa0308780:CDS
ATATCTAAGATGGGTTTACG+TGG 0.723343 2_4:+3215854 Msa0308780:CDS
GCGTCTCCTACTACTCAGCG+GGG 0.736864 2_4:+3218488 Msa0308780:three_prime_UTR
AACGATGGCTAATGACACGT+AGG 0.739267 2_4:-3217215 None:intergenic
GAGAGTCTCAAGATTCAACG+TGG 0.795417 2_4:+3216125 Msa0308780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGATATTATTCAATTTAA+GGG + chr2_4:3217640-3217659 Msa0308780:intron 10.0%
!! AATATTATAATTAATTATCC+AGG + chr2_4:3218646-3218665 Msa0308780:three_prime_UTR 10.0%
!! ACATAAAAAGTTTGAATAAT+TGG - chr2_4:3215682-3215701 None:intergenic 15.0%
!!! TAATAATTGTATGGATTATA+TGG + chr2_4:3216404-3216423 Msa0308780:intron 15.0%
!!! TAGTTATAACTAATTTTCAT+TGG + chr2_4:3216725-3216744 Msa0308780:intron 15.0%
!!! AGTTATAACTAATTTTCATT+GGG + chr2_4:3216726-3216745 Msa0308780:intron 15.0%
!!! TTACTAAATATATCTTTTAC+TGG - chr2_4:3217509-3217528 None:intergenic 15.0%
!! GATTGATATTATTCAATTTA+AGG + chr2_4:3217639-3217658 Msa0308780:intron 15.0%
!! TAAAAAGTTTGAATAATTGG+TGG - chr2_4:3215679-3215698 None:intergenic 20.0%
!!! AAACTTTTTATGTGTTGATT+TGG + chr2_4:3215689-3215708 Msa0308780:five_prime_UTR 20.0%
!!! GAAAACACATAAAATTTTGA+AGG + chr2_4:3215737-3215756 Msa0308780:five_prime_UTR 20.0%
!!! GTTTTTTTCTTTCTTCTATT+TGG + chr2_4:3216173-3216192 Msa0308780:intron 20.0%
!! TCTATTTGATGAATAGTTAT+AGG + chr2_4:3216332-3216351 Msa0308780:intron 20.0%
!!! ATCCTAATCTTTTTCAATTT+GGG - chr2_4:3216385-3216404 None:intergenic 20.0%
!!! TATCCTAATCTTTTTCAATT+TGG - chr2_4:3216386-3216405 None:intergenic 20.0%
!! AGATTAGGATAATAATTGTA+TGG + chr2_4:3216395-3216414 Msa0308780:intron 20.0%
!!! TATATGGTTAATTTGATTGA+AGG + chr2_4:3216420-3216439 Msa0308780:intron 20.0%
!!! ATATGGTTAATTTGATTGAA+GGG + chr2_4:3216421-3216440 Msa0308780:intron 20.0%
!! TGATGCAATAAAAAACATAA+TGG - chr2_4:3216799-3216818 None:intergenic 20.0%
!! ATTTCACATTTCTTAAATGA+AGG - chr2_4:3217374-3217393 None:intergenic 20.0%
!!! TAAATATATCTTTTACTGGA+AGG - chr2_4:3217505-3217524 None:intergenic 20.0%
!!! AATTTGCTATTTCACAAATT+TGG + chr2_4:3217666-3217685 Msa0308780:intron 20.0%
!! CACTAAAAATAGATTGTAAT+AGG + chr2_4:3217743-3217762 Msa0308780:intron 20.0%
!! AATAAATTTCCATTTACCTA+AGG - chr2_4:3218092-3218111 None:intergenic 20.0%
!! TATAAAACTTCAATTTCCTT+TGG + chr2_4:3218716-3218735 Msa0308780:three_prime_UTR 20.0%
!!! TATGTGTTGATTTGGTTAAT+TGG + chr2_4:3215697-3215716 Msa0308780:five_prime_UTR 25.0%
!!! ACATAAAATTTTGAAGGCTA+TGG + chr2_4:3215743-3215762 Msa0308780:exon 25.0%
!!! AATTCTGGAATATATCATGA+TGG + chr2_4:3215807-3215826 Msa0308780:CDS 25.0%
!!! TTTTATTAGTCTCTCCAAAT+GGG + chr2_4:3216052-3216071 Msa0308780:CDS 25.0%
!!! ATCGTAAATATGGTTTTAGT+AGG + chr2_4:3216075-3216094 Msa0308780:CDS 25.0%
! AAGCATGCATCAAAATTAAA+CGG - chr2_4:3216240-3216259 None:intergenic 25.0%
!! ATGAACAATGAATTTTGTCA+TGG + chr2_4:3216273-3216292 Msa0308780:intron 25.0%
!! TATGGTTAATTTGATTGAAG+GGG + chr2_4:3216422-3216441 Msa0308780:intron 25.0%
! ACAACATCTTGTGTAAAAAT+AGG + chr2_4:3216491-3216510 Msa0308780:intron 25.0%
! TCTTGTGTAAAAATAGGATA+AGG + chr2_4:3216497-3216516 Msa0308780:intron 25.0%
!! TTTTTCTGTTGAACAAGATT+CGG - chr2_4:3216581-3216600 None:intergenic 25.0%
! ATCTTGTTCAACAGAAAAAT+AGG + chr2_4:3216582-3216601 Msa0308780:intron 25.0%
! CTTATGTAACTATTTGACAA+TGG - chr2_4:3216767-3216786 None:intergenic 25.0%
! CATTGTCAAATAGTTACATA+AGG + chr2_4:3216765-3216784 Msa0308780:intron 25.0%
! GATTTCTATTTCAGTTAGAA+TGG - chr2_4:3216836-3216855 None:intergenic 25.0%
!!! TCTGTTTCTTTTTGAAAAAC+AGG - chr2_4:3217153-3217172 None:intergenic 25.0%
!!! TGTTTTTCAAAAAGAAACAG+AGG + chr2_4:3217152-3217171 Msa0308780:CDS 25.0%
!!! ATTTACTCATTTCTAGTGTT+TGG - chr2_4:3217441-3217460 None:intergenic 25.0%
! TCTAAACCTATGAAAACTTA+CGG + chr2_4:3217536-3217555 Msa0308780:intron 25.0%
! GACAAACATAACATTATTAG+TGG + chr2_4:3217589-3217608 Msa0308780:intron 25.0%
! TTTAGACCTTGAAATTGTAA+GGG - chr2_4:3217709-3217728 None:intergenic 25.0%
!! TTTTAGACCTTGAAATTGTA+AGG - chr2_4:3217710-3217729 None:intergenic 25.0%
! GAAATAGTATGTGTTTGAAT+TGG - chr2_4:3218052-3218071 None:intergenic 25.0%
!! TTTTATTGCAGTGACATATA+TGG + chr2_4:3218160-3218179 Msa0308780:intron 25.0%
!! CATAAACTTTTTATTCGTAG+CGG - chr2_4:3218602-3218621 None:intergenic 25.0%
!! GCATGTTTGTTAAATTGTTA+TGG + chr2_4:3218678-3218697 Msa0308780:three_prime_UTR 25.0%
TTCAAATGTCTCTAACTATC+AGG - chr2_4:3215581-3215600 None:intergenic 30.0%
AGAAAACAATGCTGATATTG+CGG - chr2_4:3215616-3215635 None:intergenic 30.0%
!!! AGAAAGAGAGGTTAGTTTTT+TGG + chr2_4:3215770-3215789 Msa0308780:CDS 30.0%
GCAATGAACAAAGAAAATTC+TGG + chr2_4:3215792-3215811 Msa0308780:CDS 30.0%
! ATCATGATGGAAAAGAATTG+AGG + chr2_4:3215820-3215839 Msa0308780:CDS 30.0%
! ATTCAGAGCATATCTAAGAT+GGG + chr2_4:3215845-3215864 Msa0308780:CDS 30.0%
CAGCTTTGCATAAATTTGAT+TGG - chr2_4:3215881-3215900 None:intergenic 30.0%
CAATCAAATTTATGCAAAGC+TGG + chr2_4:3215879-3215898 Msa0308780:CDS 30.0%
AGTAAAAAGTGAGACAGAAT+AGG - chr2_4:3215945-3215964 None:intergenic 30.0%
!! CTTTTATTAGTCTCTCCAAA+TGG + chr2_4:3216051-3216070 Msa0308780:CDS 30.0%
TGCCCAAATTGAAAAAGATT+AGG + chr2_4:3216380-3216399 Msa0308780:intron 30.0%
CATACAATACATCAAATGGT+GGG + chr2_4:3216523-3216542 Msa0308780:intron 30.0%
! TTTTCTGTTGAACAAGATTC+GGG - chr2_4:3216580-3216599 None:intergenic 30.0%
! TCAATAGTGTGTTCTCTTTT+TGG - chr2_4:3216691-3216710 None:intergenic 30.0%
ACACATTGACATATGAAACA+GGG - chr2_4:3216940-3216959 None:intergenic 30.0%
ACATACATCTCACCATAATA+TGG - chr2_4:3217003-3217022 None:intergenic 30.0%
! TAGAACCTCAATTTTCTTCT+TGG + chr2_4:3217068-3217087 Msa0308780:CDS 30.0%
TTTCAAAAAGAAACAGAGGT+AGG + chr2_4:3217156-3217175 Msa0308780:CDS 30.0%
!! TTGCTATTTCACAAATTTGG+AGG + chr2_4:3217669-3217688 Msa0308780:intron 30.0%
AAGATTGTCAAAGGAGTTTA+TGG - chr2_4:3217906-3217925 None:intergenic 30.0%
! TGTTTGAATTGGCATCATTT+TGG - chr2_4:3218041-3218060 None:intergenic 30.0%
TATTTCATTCCATAAGAGAC+AGG + chr2_4:3218066-3218085 Msa0308780:CDS 30.0%
ATATATGGAGAACAATAGAG+CGG + chr2_4:3218175-3218194 Msa0308780:CDS 30.0%
!!! CTCCATTTTTGGTATTCAAT+TGG + chr2_4:3218241-3218260 Msa0308780:CDS 30.0%
AGCCAATTGAATACCAAAAA+TGG - chr2_4:3218246-3218265 None:intergenic 30.0%
ACAAAAAGATGACTGTTCAA+AGG - chr2_4:3218372-3218391 None:intergenic 30.0%
TTGTTTGAATCTCATTGTTC+TGG + chr2_4:3218388-3218407 Msa0308780:three_prime_UTR 30.0%
! AGTGTTTGTTGCTATGTATA+TGG + chr2_4:3218570-3218589 Msa0308780:three_prime_UTR 30.0%
GATTCAGAGCATATCTAAGA+TGG + chr2_4:3215844-3215863 Msa0308780:CDS 35.0%
! ATATCTAAGATGGGTTTACG+TGG + chr2_4:3215854-3215873 Msa0308780:CDS 35.0%
TATGCAAAGCTGGTATATCT+TGG + chr2_4:3215889-3215908 Msa0308780:CDS 35.0%
! TTGGTCTGTGTTTTTCACTT+TGG + chr2_4:3215908-3215927 Msa0308780:CDS 35.0%
ACAGAAAGAGAAATCTGAAC+AGG - chr2_4:3216017-3216036 None:intergenic 35.0%
TCCAAATGGGATCGTAAATA+TGG + chr2_4:3216065-3216084 Msa0308780:CDS 35.0%
ACCATATTTACGATCCCATT+TGG - chr2_4:3216069-3216088 None:intergenic 35.0%
GAGACAAACACAATTACACA+AGG - chr2_4:3216302-3216321 None:intergenic 35.0%
!! TTACACAAGATGTTGTTTCC+AGG - chr2_4:3216487-3216506 None:intergenic 35.0%
GCTGCATACAATACATCAAA+TGG + chr2_4:3216519-3216538 Msa0308780:intron 35.0%
GCATACAATACATCAAATGG+TGG + chr2_4:3216522-3216541 Msa0308780:intron 35.0%
! CACAAGGAGAGTTATGTTTT+AGG + chr2_4:3216626-3216645 Msa0308780:intron 35.0%
TGTGCAGATTATGCAACTAA+AGG - chr2_4:3216668-3216687 None:intergenic 35.0%
GACACATTGACATATGAAAC+AGG - chr2_4:3216941-3216960 None:intergenic 35.0%
GTCAATGTGTCTACAAGATA+TGG + chr2_4:3216950-3216969 Msa0308780:CDS 35.0%
AATGTGTCTACAAGATATGG+TGG + chr2_4:3216953-3216972 Msa0308780:CDS 35.0%
GTATCCCAGATACCATATTA+TGG + chr2_4:3216988-3217007 Msa0308780:CDS 35.0%
CTCACCATAATATGGTATCT+GGG - chr2_4:3216995-3217014 None:intergenic 35.0%
TCTCACCATAATATGGTATC+TGG - chr2_4:3216996-3217015 None:intergenic 35.0%
GCATACCAAGAAGAAAATTG+AGG - chr2_4:3217076-3217095 None:intergenic 35.0%
CTACCTTCAAATACAAAGAC+TGG + chr2_4:3217116-3217135 Msa0308780:CDS 35.0%
CATCCAGTCTTTGTATTTGA+AGG - chr2_4:3217122-3217141 None:intergenic 35.0%
AAATGAAAGCTCGAAAACGA+TGG - chr2_4:3217233-3217252 None:intergenic 35.0%
! CATTTTGTCTTCTCTGCTTT+TGG + chr2_4:3217248-3217267 Msa0308780:CDS 35.0%
CCATAAGTAGAAAAATGAGC+TGG - chr2_4:3217284-3217303 None:intergenic 35.0%
!! CCAGCTCATTTTTCTACTTA+TGG + chr2_4:3217281-3217300 Msa0308780:CDS 35.0%
AGCAAAAGAACGCAAAAGAA+CGG - chr2_4:3217401-3217420 None:intergenic 35.0%
! CTCAATCCGTAAGTTTTCAT+AGG - chr2_4:3217545-3217564 None:intergenic 35.0%
TATGTAAAGTCCACTAACCA+AGG - chr2_4:3217804-3217823 None:intergenic 35.0%
AACTCCTTTGACAATCTTGA+TGG + chr2_4:3217908-3217927 Msa0308780:CDS 35.0%
TATATGGAGAACAATAGAGC+GGG + chr2_4:3218176-3218195 Msa0308780:CDS 35.0%
! TTCAATTGGCTCTTTGTCTT+TGG + chr2_4:3218255-3218274 Msa0308780:CDS 35.0%
! CGAATAGTGTTGAGACTATT+GGG + chr2_4:3218448-3218467 Msa0308780:three_prime_UTR 35.0%
TGTCTCTAACTATCAGGTTC+TGG - chr2_4:3215575-3215594 None:intergenic 40.0%
ATCTGAACAGGAACATGGTA+CGG - chr2_4:3216005-3216024 None:intergenic 40.0%
GAGAAATCTGAACAGGAACA+TGG - chr2_4:3216010-3216029 None:intergenic 40.0%
TGGCTATGCTCAACAAATGA+AGG + chr2_4:3216145-3216164 Msa0308780:CDS 40.0%
TGGGCAAAAACGTTGAATTG+TGG - chr2_4:3216366-3216385 None:intergenic 40.0%
TGCAACGGTAAAGTTGTTGT+CGG + chr2_4:3216453-3216472 Msa0308780:intron 40.0%
CATGAAGCTCATCTTGCTTT+GGG + chr2_4:3216909-3216928 Msa0308780:CDS 40.0%
ATGAAGCTCATCTTGCTTTG+GGG + chr2_4:3216910-3216929 Msa0308780:CDS 40.0%
! ATATGGTATCTGGGATACTC+CGG - chr2_4:3216986-3217005 None:intergenic 40.0%
AGAGGTAGGAACAATCTTGT+TGG + chr2_4:3217170-3217189 Msa0308780:CDS 40.0%
GTAGGTTCCTTCTTATTCTC+AGG - chr2_4:3217200-3217219 None:intergenic 40.0%
AGGATATCAACATCAGCATG+TGG - chr2_4:3217315-3217334 None:intergenic 40.0%
TGCTGATGTTGATATCCTCA+AGG + chr2_4:3217317-3217336 Msa0308780:CDS 40.0%
GCTGATGTTGATATCCTCAA+GGG + chr2_4:3217318-3217337 Msa0308780:CDS 40.0%
GACAGACCCTTACAATTTCA+AGG + chr2_4:3217700-3217719 Msa0308780:intron 40.0%
CTCACCATCAAGATTGTCAA+AGG - chr2_4:3217915-3217934 None:intergenic 40.0%
TATGGCTGCCGATATTAGTT+GGG + chr2_4:3217967-3217986 Msa0308780:CDS 40.0%
AGAGGATCCCCAACTAATAT+CGG - chr2_4:3217978-3217997 None:intergenic 40.0%
ATCTCCTGATTCATCTTCAG+AGG - chr2_4:3217996-3218015 None:intergenic 40.0%
AGATGAATCAGGAGATGAAG+AGG + chr2_4:3218000-3218019 Msa0308780:CDS 40.0%
GATTCATGCTTGACAGAACT+TGG + chr2_4:3218213-3218232 Msa0308780:CDS 40.0%
! ACTTGGCTTCACTCCATTTT+TGG + chr2_4:3218230-3218249 Msa0308780:CDS 40.0%
GTTGGTATCTAGAGACAGAA+AGG + chr2_4:3218293-3218312 Msa0308780:exon 40.0%
TACTGTACATTGCACAAGGT+AGG - chr2_4:3218346-3218365 None:intergenic 40.0%
GATGTACTGTACATTGCACA+AGG - chr2_4:3218350-3218369 None:intergenic 40.0%
! GCGAATAGTGTTGAGACTAT+TGG + chr2_4:3218447-3218466 Msa0308780:three_prime_UTR 40.0%
CAAACATGCAAACAAATGCC+TGG - chr2_4:3218667-3218686 None:intergenic 40.0%
GGCTATGGAAGAAGAAAGAG+AGG + chr2_4:3215758-3215777 Msa0308780:exon 45.0%
GAGAGTCTCAAGATTCAACG+TGG + chr2_4:3216125-3216144 Msa0308780:CDS 45.0%
GCAACGGTAAAGTTGTTGTC+GGG + chr2_4:3216454-3216473 Msa0308780:intron 45.0%
CCAAAGCAAGATGAGCTTCA+TGG - chr2_4:3216911-3216930 None:intergenic 45.0%
CCATGAAGCTCATCTTGCTT+TGG + chr2_4:3216908-3216927 Msa0308780:CDS 45.0%
AAGATATGGTGGTACGTCTC+CGG + chr2_4:3216964-3216983 Msa0308780:CDS 45.0%
CAGCATCATTCTGTGCACAT+TGG + chr2_4:3217026-3217045 Msa0308780:CDS 45.0%
GCACATTGGAGCTATGTTCA+TGG + chr2_4:3217040-3217059 Msa0308780:CDS 45.0%
! TGGAGCACCTGAGAATAAGA+AGG + chr2_4:3217190-3217209 Msa0308780:CDS 45.0%
AACGATGGCTAATGACACGT+AGG - chr2_4:3217218-3217237 None:intergenic 45.0%
!! GTCTTCTCTGCTTTTGGTGA+CGG + chr2_4:3217254-3217273 Msa0308780:CDS 45.0%
GATGTTGATATCCTCAAGGG+TGG + chr2_4:3217321-3217340 Msa0308780:CDS 45.0%
AAGTCCACTAACCAAGGTGA+TGG - chr2_4:3217798-3217817 None:intergenic 45.0%
CATAAAGCACAATCCCTCAC+AGG + chr2_4:3217851-3217870 Msa0308780:CDS 45.0%
GATAGCTTCAGCACAAGCTA+TGG + chr2_4:3217949-3217968 Msa0308780:CDS 45.0%
CTATGGCTGCCGATATTAGT+TGG + chr2_4:3217966-3217985 Msa0308780:CDS 45.0%
ATGGCTGCCGATATTAGTTG+GGG + chr2_4:3217968-3217987 Msa0308780:CDS 45.0%
! GGATCCTCTGAAGATGAATC+AGG + chr2_4:3217989-3218008 Msa0308780:CDS 45.0%
TTCCATAAGAGACAGGCCTT+AGG + chr2_4:3218073-3218092 Msa0308780:CDS 45.0%
TACCTAAGGCCTGTCTCTTA+TGG - chr2_4:3218078-3218097 None:intergenic 45.0%
AGAGACAGGCCTTAGGTAAA+TGG + chr2_4:3218080-3218099 Msa0308780:intron 45.0%
AGAGCGGGAATCACAGTTTA+TGG + chr2_4:3218191-3218210 Msa0308780:CDS 45.0%
TGGCTACTGAACAAGACTGT+TGG + chr2_4:3218275-3218294 Msa0308780:CDS 45.0%
ACATTGCACAAGGTAGGACT+AGG - chr2_4:3218340-3218359 None:intergenic 45.0%
AGCGGGGAATATCCTCATAT+CGG + chr2_4:3218504-3218523 Msa0308780:three_prime_UTR 45.0%
GAAGGGGAGTCTTGATGCAA+CGG + chr2_4:3216438-3216457 Msa0308780:intron 50.0%
! CAAATGGTGGGACCTCTTCT+CGG + chr2_4:3216535-3216554 Msa0308780:intron 50.0%
!! CCTTGTGCTGCTTCTGTTTC+TGG - chr2_4:3216613-3216632 None:intergenic 50.0%
CCAGAAACAGAAGCAGCACA+AGG + chr2_4:3216610-3216629 Msa0308780:intron 50.0%
TGCGTCCTCTTTCGACTCAT+TGG + chr2_4:3217093-3217112 Msa0308780:CDS 50.0%
GGTAGCCAATGAGTCGAAAG+AGG - chr2_4:3217101-3217120 None:intergenic 50.0%
! GCAGTTAGTCTCCATCACCT+TGG + chr2_4:3217784-3217803 Msa0308780:intron 50.0%
GTCTCCATCACCTTGGTTAG+TGG + chr2_4:3217791-3217810 Msa0308780:CDS 50.0%
CTTAGCAGCAAGACCTGTGA+GGG - chr2_4:3217867-3217886 None:intergenic 50.0%
ACTTAGCAGCAAGACCTGTG+AGG - chr2_4:3217868-3217887 None:intergenic 50.0%
TCACAGGTCTTGCTGCTAAG+TGG + chr2_4:3217867-3217886 Msa0308780:CDS 50.0%
GCTGAAGCTATCAGTGCTGT+AGG - chr2_4:3217941-3217960 None:intergenic 50.0%
AAGGTAGGACTAGGACTAGG+AGG - chr2_4:3218331-3218350 None:intergenic 50.0%
CACAAGGTAGGACTAGGACT+AGG - chr2_4:3218334-3218353 None:intergenic 50.0%
GATATTCCCCGCTGAGTAGT+AGG - chr2_4:3218497-3218516 None:intergenic 50.0%
TTGTTGTCGGGCTCAAGTCC+TGG + chr2_4:3216466-3216485 Msa0308780:intron 55.0%
!! AAGCTCTAGGGTCCGAGAAG+AGG - chr2_4:3216550-3216569 None:intergenic 55.0%
CTTCTCGGACCCTAGAGCTT+CGG + chr2_4:3216550-3216569 Msa0308780:intron 55.0%
TCGGGTGATCCGAAGCTCTA+GGG - chr2_4:3216562-3216581 None:intergenic 55.0%
TTCGGGTGATCCGAAGCTCT+AGG - chr2_4:3216563-3216582 None:intergenic 55.0%
ATCCTCAAGGGTGGAGAGCT+TGG + chr2_4:3217330-3217349 Msa0308780:CDS 55.0%
TCCTCAAGGGTGGAGAGCTT+GGG + chr2_4:3217331-3217350 Msa0308780:CDS 55.0%
CTGCGTCTCCTACTACTCAG+CGG + chr2_4:3218486-3218505 Msa0308780:three_prime_UTR 55.0%
TGCGTCTCCTACTACTCAGC+GGG + chr2_4:3218487-3218506 Msa0308780:three_prime_UTR 55.0%
CCTGCGTGTCATCCGATATG+AGG - chr2_4:3218519-3218538 None:intergenic 55.0%
CCTCATATCGGATGACACGC+AGG + chr2_4:3218516-3218535 Msa0308780:three_prime_UTR 55.0%
TATCGGATGACACGCAGGAC+AGG + chr2_4:3218521-3218540 Msa0308780:three_prime_UTR 55.0%
CCTCAAGGGTGGAGAGCTTG+GGG + chr2_4:3217332-3217351 Msa0308780:CDS 60.0%
GCGTCTCCTACTACTCAGCG+GGG + chr2_4:3218488-3218507 Msa0308780:three_prime_UTR 60.0%
CCCCAAGCTCTCCACCCTTG+AGG - chr2_4:3217335-3217354 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 3215533 3218740 3215533 ID=Msa0308780;Name=Msa0308780
chr2_4 mRNA 3215533 3218740 3215533 ID=Msa0308780-mRNA-1;Parent=Msa0308780;Name=Msa0308780-mRNA-1;_AED=0.03;_eAED=0.03;_QI=229|1|1|1|1|1|4|436|431
chr2_4 exon 3215533 3216166 3215533 ID=Msa0308780-mRNA-1:exon:928;Parent=Msa0308780-mRNA-1
chr2_4 exon 3216904 3217353 3216904 ID=Msa0308780-mRNA-1:exon:929;Parent=Msa0308780-mRNA-1
chr2_4 exon 3217788 3218094 3217788 ID=Msa0308780-mRNA-1:exon:930;Parent=Msa0308780-mRNA-1
chr2_4 exon 3218171 3218740 3218171 ID=Msa0308780-mRNA-1:exon:931;Parent=Msa0308780-mRNA-1
chr2_4 five_prime_UTR 3215533 3215761 3215533 ID=Msa0308780-mRNA-1:five_prime_utr;Parent=Msa0308780-mRNA-1
chr2_4 CDS 3215762 3216166 3215762 ID=Msa0308780-mRNA-1:cds;Parent=Msa0308780-mRNA-1
chr2_4 CDS 3216904 3217353 3216904 ID=Msa0308780-mRNA-1:cds;Parent=Msa0308780-mRNA-1
chr2_4 CDS 3217788 3218094 3217788 ID=Msa0308780-mRNA-1:cds;Parent=Msa0308780-mRNA-1
chr2_4 CDS 3218171 3218304 3218171 ID=Msa0308780-mRNA-1:cds;Parent=Msa0308780-mRNA-1
chr2_4 three_prime_UTR 3218305 3218740 3218305 ID=Msa0308780-mRNA-1:three_prime_utr;Parent=Msa0308780-mRNA-1
Gene Sequence

>Msa0308780

ATGGAAGAAGAAAGAGAGGTTAGTTTTTTGGCAATGAACAAAGAAAATTCTGGAATATATCATGATGGAAAAGAATTGAGGAGATTCAGAGCATATCTAAGATGGGTTTACGTGGACCAATCAAATTTATGCAAAGCTGGTATATCTTGGTCTGTGTTTTTCACTTTGGCTTACGTTGTACCTATTCTGTCTCACTTTTTACTTGATTGTTCGACAACGTGTGATGCTGATCATCGTAGACCGTACCATGTTCCTGTTCAGATTTCTCTTTCTGTCTTTGCTACTTTGTCTTTTATTAGTCTCTCCAAATGGGATCGTAAATATGGTTTTAGTAGGTTTCTTTTTCTTGATAAAGTTAGTGATGAGAGTCTCAAGATTCAACGTGGCTATGCTCAACAAATGAAGAGAACCATGAAGCTCATCTTGCTTTGGGGACTTCCCTGTTTCATATGTCAATGTGTCTACAAGATATGGTGGTACGTCTCCGGAGTATCCCAGATACCATATTATGGTGAGATGTATGTGAGCAGCATCATTCTGTGCACATTGGAGCTATGTTCATGGTTTTATAGAACCTCAATTTTCTTCTTGGTATGCGTCCTCTTTCGACTCATTGGCTACCTTCAAATACAAAGACTGGATGAATTTGCTCCTGTTTTTCAAAAAGAAACAGAGGTAGGAACAATCTTGTTGGAGCACCTGAGAATAAGAAGGAACCTACGTGTCATTAGCCATCGTTTTCGAGCTTTCATTTTGTCTTCTCTGCTTTTGGTGACGGCAAGCCAGCTCATTTTTCTACTTATGGTTATAAAACCACATGCTGATGTTGATATCCTCAAGGGTGGAGAGCTTGGGTTAGTCTCCATCACCTTGGTTAGTGGACTTTACATACTCTTACGAAGCGCAACGAAGATCACACATAAAGCACAATCCCTCACAGGTCTTGCTGCTAAGTGGCATATATGTGCTACCATAAACTCCTTTGACAATCTTGATGGTGAGACACCTACAGCACTGATAGCTTCAGCACAAGCTATGGCTGCCGATATTAGTTGGGGATCCTCTGAAGATGAATCAGGAGATGAAGAGGATGAGTTGAATAACACCAAAATGATGCCAATTCAAACACATACTATTTCATTCCATAAGAGACAGGCCTTAGTGACATATATGGAGAACAATAGAGCGGGAATCACAGTTTATGGATTCATGCTTGACAGAACTTGGCTTCACTCCATTTTTGGTATTCAATTGGCTCTTTGTCTTTGGCTACTGAACAAGACTGTTGGTATCTAG
Protein sequence

>Msa0308780

MEEEREVSFLAMNKENSGIYHDGKELRRFRAYLRWVYVDQSNLCKAGISWSVFFTLAYVVPILSHFLLDCSTTCDADHRRPYHVPVQISLSVFATLSFISLSKWDRKYGFSRFLFLDKVSDESLKIQRGYAQQMKRTMKLILLWGLPCFICQCVYKIWWYVSGVSQIPYYGEMYVSSIILCTLELCSWFYRTSIFFLVCVLFRLIGYLQIQRLDEFAPVFQKETEVGTILLEHLRIRRNLRVISHRFRAFILSSLLLVTASQLIFLLMVIKPHADVDILKGGELGLVSITLVSGLYILLRSATKITHKAQSLTGLAAKWHICATINSFDNLDGETPTALIASAQAMAADISWGSSEDESGDEEDELNNTKMMPIQTHTISFHKRQALVTYMENNRAGITVYGFMLDRTWLHSIFGIQLALCLWLLNKTVGI*