AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0202030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0202030 MtrunA17_Chr2g0298851 92.880 618 44 0 1 618 1 618 0.0 1186
Msa0202030 MtrunA17_Chr7g0225671 79.772 613 121 3 6 617 13 623 0.0 1004
Msa0202030 MtrunA17_Chr7g0223851 78.768 617 129 2 1 616 10 625 0.0 1002
Msa0202030 MtrunA17_Chr7g0223721 74.960 619 153 2 1 618 1 618 0.0 982
Msa0202030 MtrunA17_Chr4g0040931 74.434 618 156 2 1 617 1 617 0.0 968
Msa0202030 MtrunA17_Chr2g0298861 73.132 629 157 3 1 617 1 629 0.0 956
Msa0202030 MtrunA17_Chr7g0223821 76.737 619 137 5 1 614 1 617 0.0 947
Msa0202030 MtrunA17_Chr7g0223821 61.905 84 29 2 534 614 620 703 1.08e-18 90.5
Msa0202030 MtrunA17_Chr7g0223831 75.121 619 124 3 1 618 1 590 0.0 945
Msa0202030 MtrunA17_Chr7g0221661 74.309 615 155 3 1 614 1 613 0.0 943
Msa0202030 MtrunA17_Chr4g0012541 74.554 617 146 3 1 616 1 607 0.0 939
Msa0202030 MtrunA17_Chr2g0293581 73.226 620 162 4 1 617 1 619 0.0 937
Msa0202030 MtrunA17_Chr7g0215261 75.201 621 115 6 1 618 1 585 0.0 929
Msa0202030 MtrunA17_Chr5g0427901 72.452 628 159 4 1 616 1 626 0.0 924
Msa0202030 MtrunA17_Chr7g0233311 72.536 619 167 3 1 617 1 618 0.0 920
Msa0202030 MtrunA17_Chr7g0223801 72.771 617 163 5 1 616 1 613 0.0 913
Msa0202030 MtrunA17_Chr7g0228281 74.152 619 111 7 1 617 62 633 0.0 911
Msa0202030 MtrunA17_Chr2g0287631 72.213 619 169 3 1 617 1 618 0.0 906
Msa0202030 MtrunA17_Chr7g0217971 71.036 618 151 5 1 617 1 591 0.0 870
Msa0202030 MtrunA17_Chr7g0233301 69.467 619 155 3 1 617 1 587 0.0 867
Msa0202030 MtrunA17_Chr8g0390821 63.881 670 171 9 1 616 1 653 0.0 825
Msa0202030 MtrunA17_Chr7g0223711 64.725 618 213 5 1 616 1 615 0.0 820
Msa0202030 MtrunA17_Chr7g0223751 64.668 617 214 4 1 616 1 614 0.0 815
Msa0202030 MtrunA17_Chr7g0223181 65.428 619 207 6 1 616 1 615 0.0 812
Msa0202030 MtrunA17_Chr7g0223031 65.025 609 209 4 1 608 1 606 0.0 810
Msa0202030 MtrunA17_Chr8g0350731 64.830 617 213 4 1 616 29 642 0.0 810
Msa0202030 MtrunA17_Chr2g0296391 72.023 529 146 2 1 528 1 528 0.0 808
Msa0202030 MtrunA17_Chr7g0223781 65.366 615 208 4 1 614 1 611 0.0 808
Msa0202030 MtrunA17_Chr7g0223731 63.695 617 220 4 1 616 1 614 0.0 807
Msa0202030 MtrunA17_Chr7g0223741 67.343 591 189 4 1 589 1 589 0.0 806
Msa0202030 MtrunA17_Chr7g0223621 63.799 616 219 4 1 615 1 613 0.0 803
Msa0202030 MtrunA17_Chr4g0031321 64.459 619 213 6 1 616 1 615 0.0 803
Msa0202030 MtrunA17_Chr4g0031301 64.459 619 213 6 1 616 1 615 0.0 803
Msa0202030 MtrunA17_Chr7g0262821 64.136 619 215 6 1 616 1 615 0.0 796
Msa0202030 MtrunA17_Chr7g0223291 63.813 619 217 6 1 616 1 615 0.0 795
Msa0202030 MtrunA17_Chr4g0073741 64.136 619 214 7 1 616 1 614 0.0 795
Msa0202030 MtrunA17_Chr7g0223701 63.387 620 216 6 1 611 1 618 0.0 787
Msa0202030 MtrunA17_Chr7g0248131 63.124 621 222 7 1 616 1 619 0.0 781
Msa0202030 MtrunA17_Chr7g0248151 63.651 619 220 5 1 616 1 617 0.0 779
Msa0202030 MtrunA17_Chr7g0223631 62.723 617 212 6 1 616 1 600 0.0 769
Msa0202030 MtrunA17_Chr1g0166551 60.976 615 125 6 6 618 2 503 0.0 727
Msa0202030 MtrunA17_Chr2g0293601 68.654 520 127 3 1 519 1 485 0.0 724
Msa0202030 MtrunA17_Chr2g0331381 66.976 539 115 4 10 548 7 482 0.0 712
Msa0202030 MtrunA17_Chr7g0223541 63.303 545 195 5 74 616 31 572 0.0 693
Msa0202030 MtrunA17_Chr6g0462681 56.926 592 250 4 1 590 1 589 0.0 669
Msa0202030 MtrunA17_Chr7g0223681 63.378 527 189 4 91 616 2 525 0.0 665
Msa0202030 MtrunA17_Chr6g0462601 57.504 593 245 6 1 590 1 589 0.0 661
Msa0202030 MtrunA17_Chr6g0462711 55.519 616 262 8 9 616 10 621 0.0 644
Msa0202030 MtrunA17_Chr2g0293561 79.412 374 77 0 1 374 1 374 0.0 642
Msa0202030 MtrunA17_Chr2g0284681 54.457 617 256 9 5 613 4 603 0.0 636
Msa0202030 MtrunA17_Chr8g0388761 55.932 590 248 8 7 591 36 618 0.0 627
Msa0202030 MtrunA17_Chr8g0388671 55.932 590 248 8 7 591 36 618 0.0 624
Msa0202030 MtrunA17_Chr6g0462641 55.017 598 257 7 1 590 1 594 0.0 622
Msa0202030 MtrunA17_Chr6g0462771 54.545 616 267 8 9 616 9 619 0.0 622
Msa0202030 MtrunA17_Chr6g0462611 55.684 519 225 5 74 590 22 537 0.0 568
Msa0202030 MtrunA17_Chr2g0299921 69.797 394 115 2 3 396 1023 1412 0.0 561
Msa0202030 MtrunA17_Chr2g0299921 67.513 394 123 3 3 395 1407 1796 1.79e-167 522
Msa0202030 MtrunA17_Chr2g0293271 61.187 438 99 3 21 458 43 409 0.0 527
Msa0202030 MtrunA17_Chr2g0299821 58.850 452 170 4 1 438 1 450 0.0 525
Msa0202030 MtrunA17_Chr2g0299821 49.515 103 40 4 444 538 384 482 9.04e-17 83.6
Msa0202030 MtrunA17_Chr2g0299851 67.095 389 124 2 1 389 1030 1414 1.74e-175 535
Msa0202030 MtrunA17_Chr1g0151991 81.443 291 54 0 1 291 1 291 2.10e-175 499
Msa0202030 MtrunA17_Chr2g0299791 66.581 389 126 2 1 389 1034 1418 3.43e-173 530
Msa0202030 MtrunA17_Chr2g0299881 64.286 392 132 3 2 389 1031 1418 8.13e-166 511
Msa0202030 MtrunA17_Chr2g0299881 54.795 73 31 1 180 250 1403 1475 2.60e-16 83.2
Msa0202030 MtrunA17_Chr2g0279901 46.552 580 281 15 10 581 57 615 1.17e-163 483
Msa0202030 MtrunA17_Chr6g0462861 44.783 623 309 12 6 616 6 605 1.72e-161 477
Msa0202030 MtrunA17_Chr4g0049501 45.172 580 290 14 10 581 54 613 1.90e-158 470
Msa0202030 MtrunA17_Chr7g0223761 62.533 379 97 5 241 617 3 338 2.10e-158 458
Msa0202030 MtrunA17_Chr1g0152001 75.082 305 72 4 315 618 1 302 2.39e-158 457
Msa0202030 MtrunA17_Chr7g0245521 78.022 273 60 0 1 273 1 273 4.53e-158 454
Msa0202030 MtrunA17_Chr2g0277021 46.524 561 278 12 8 562 70 614 3.25e-153 457
Msa0202030 MtrunA17_Chr7g0245531 68.690 313 95 3 305 616 2 312 1.07e-141 414
Msa0202030 MtrunA17_Chr1g0188601 79.058 191 40 0 92 282 1 191 3.79e-108 323
Msa0202030 MtrunA17_Chr1g0188611 61.486 296 107 2 322 617 1 289 1.84e-106 323
Msa0202030 MtrunA17_Chr7g0223671 73.016 189 51 0 74 262 8 196 1.82e-100 304
Msa0202030 MtrunA17_Chr6g0462631 50.789 317 113 3 100 415 22 296 4.75e-96 296
Msa0202030 MtrunA17_Chr6g0462751 45.879 364 134 5 60 417 2 308 5.85e-95 294
Msa0202030 MtrunA17_Chr7g0223771 82.237 152 27 0 93 244 1 152 4.68e-89 273
Msa0202030 MtrunA17_Chr5g0394391 34.615 520 308 16 9 510 29 534 9.03e-83 271
Msa0202030 MtrunA17_Chr7g0223861 86.260 131 18 0 78 208 4 134 7.73e-78 243
Msa0202030 MtrunA17_Chr4g0004331 34.091 440 274 7 10 437 4 439 2.28e-76 260
Msa0202030 MtrunA17_Chr4g0046881 80.851 141 24 1 135 275 14 151 1.81e-75 237
Msa0202030 MtrunA17_Chr2g0331901 33.257 439 277 7 10 436 4 438 4.42e-74 254
Msa0202030 MtrunA17_Chr7g0245541 59.406 202 80 2 418 618 1 201 6.70e-74 235
Msa0202030 MtrunA17_Chr5g0444001 32.653 441 288 6 10 445 4 440 7.53e-74 246
Msa0202030 MtrunA17_Chr6g0462791 63.636 176 63 1 108 283 1 175 7.86e-74 234
Msa0202030 MtrunA17_Chr3g0098761 32.334 467 307 8 10 475 4 462 1.58e-73 251
Msa0202030 MtrunA17_Chr4g0064621 34.175 515 293 17 9 492 140 639 1.47e-63 222
Msa0202030 MtrunA17_Chr3g0120101 29.677 465 300 8 12 454 29 488 8.35e-60 214
Msa0202030 MtrunA17_Chr5g0394221 39.249 293 168 5 299 589 69 353 3.85e-56 194
Msa0202030 MtrunA17_Chr6g0481391 68.852 122 35 1 38 159 13 131 1.60e-54 181
Msa0202030 MtrunA17_Chr6g0462701 53.801 171 55 1 100 270 22 168 1.71e-54 183
Msa0202030 MtrunA17_Chr4g0060241 28.797 507 321 13 10 497 21 506 3.69e-52 188
Msa0202030 MtrunA17_Chr7g0223871 66.406 128 42 1 492 618 7 134 1.77e-47 163
Msa0202030 MtrunA17_Chr4g0046871 75.490 102 24 1 337 438 7 107 8.78e-45 154
Msa0202030 MtrunA17_Chr5g0442171 32.424 330 201 9 170 488 1 319 2.26e-43 158
Msa0202030 MtrunA17_Chr6g0462691 52.448 143 66 2 322 463 1 142 1.04e-38 140
Msa0202030 MtrunA17_Chr5g0394231 34.802 227 97 4 74 299 5 181 5.65e-38 139
Msa0202030 MtrunA17_Chr1g0188591 82.192 73 13 0 1 73 1 73 1.87e-35 128
Msa0202030 MtrunA17_Chr7g0223691 78.082 73 16 0 1 73 1 73 5.77e-34 124
Msa0202030 MtrunA17_Chr6g0481381 64.583 96 33 1 516 610 75 170 8.51e-32 121
Msa0202030 MtrunA17_Chr6g0481381 72.973 74 20 0 176 249 1 74 1.18e-31 121
Msa0202030 MtrunA17_Chr7g0223881 69.880 83 24 1 281 363 2 83 6.53e-31 115
Msa0202030 MtrunA17_Chr7g0223791 61.538 91 34 1 482 571 89 179 1.25e-29 115
Msa0202030 MtrunA17_Chr7g0223791 67.901 81 18 3 56 136 18 90 3.50e-27 108
Msa0202030 MtrunA17_Chr6g0462621 65.753 73 23 1 1 73 1 71 7.28e-26 101
Msa0202030 MtrunA17_Chr7g0221651 48.810 84 43 0 215 298 1 84 4.31e-20 85.1
Msa0202030 MtrunA17_Chr6g0462801 59.091 66 22 1 322 382 1 66 6.79e-17 75.9
Msa0202030 MtrunA17_Chr4g0064651 41.935 93 52 2 397 488 29 120 2.89e-16 75.5
Msa0202030 MtrunA17_Chr2g0284661 51.190 84 39 2 368 450 61 143 2.35e-15 73.6
Msa0202030 MtrunA17_Chr5g0442161 31.373 153 98 4 9 157 28 177 3.17e-15 74.3
Msa0202030 MtrunA17_Chr7g0223661 38.261 115 27 2 315 429 1 71 4.68e-15 70.9
Msa0202030 MtrunA17_Chr2g0299891 65.116 43 15 0 315 357 1 43 1.94e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0202030 AT3G12580.1 64.782 619 211 6 1 616 1 615 0.0 808
Msa0202030 AT5G02500.1 63.489 619 219 6 1 616 1 615 0.0 794
Msa0202030 AT5G02490.1 63.166 619 221 6 1 616 1 615 0.0 788
Msa0202030 AT1G56410.1 64.401 618 213 6 1 616 1 613 0.0 787
Msa0202030 AT3G09440.1 63.651 619 218 6 1 616 1 615 0.0 787
Msa0202030 AT3G09440.4 63.651 619 218 6 1 616 1 615 0.0 787
Msa0202030 AT3G09440.2 63.651 619 218 6 1 616 1 615 0.0 787
Msa0202030 AT3G09440.3 63.651 619 218 6 1 616 1 615 0.0 787
Msa0202030 AT1G16030.1 62.359 619 225 7 1 616 1 614 0.0 776
Msa0202030 AT5G28540.1 54.649 613 265 9 7 614 35 639 0.0 634
Msa0202030 AT5G42020.1 54.486 613 266 9 7 614 35 639 0.0 632
Msa0202030 AT5G42020.3 54.486 613 266 9 7 614 40 644 0.0 631
Msa0202030 AT1G09080.2 54.120 619 268 9 7 617 40 650 0.0 619
Msa0202030 AT1G09080.1 54.412 612 267 8 7 614 50 653 0.0 619
Msa0202030 AT5G02500.2 73.008 389 103 1 1 389 1 387 0.0 605
Msa0202030 AT5G42020.2 49.837 612 241 10 7 614 35 584 0.0 551
Msa0202030 AT5G09590.1 46.114 579 284 15 10 580 60 618 1.55e-156 466
Msa0202030 AT5G49910.1 46.702 561 277 12 8 562 79 623 1.34e-153 459
Msa0202030 AT4G24280.1 45.739 575 289 13 8 576 79 636 1.67e-153 459
Msa0202030 AT4G37910.1 47.227 559 268 12 10 560 55 594 5.84e-153 456
Msa0202030 AT4G37910.2 47.227 559 268 12 10 560 55 594 5.84e-153 456
Msa0202030 AT1G79930.2 34.091 440 274 6 10 437 4 439 1.90e-78 265
Msa0202030 AT1G79930.1 34.091 440 274 6 10 437 4 439 3.93e-78 265
Msa0202030 AT1G11660.4 33.180 434 280 5 10 437 4 433 6.22e-78 263
Msa0202030 AT1G11660.3 33.180 434 280 5 10 437 4 433 6.22e-78 263
Msa0202030 AT1G11660.1 33.180 434 280 5 10 437 4 433 6.22e-78 263
Msa0202030 AT1G79920.1 33.409 440 277 5 10 437 4 439 2.05e-77 261
Msa0202030 AT1G79920.3 33.409 440 277 5 10 437 4 439 2.14e-77 261
Msa0202030 AT1G79920.2 33.409 440 277 5 10 437 4 439 2.14e-77 261
Msa0202030 AT1G79920.4 33.409 440 277 5 10 437 4 439 2.14e-77 261
Msa0202030 AT2G32120.1 33.204 515 309 13 9 508 30 524 2.02e-76 254
Msa0202030 AT2G32120.2 33.204 515 309 13 9 508 30 524 2.02e-76 254
Msa0202030 AT1G11660.2 33.166 398 258 4 46 437 1 396 6.87e-70 240
Msa0202030 AT4G16660.1 28.599 521 337 9 6 495 23 539 3.30e-62 221
Msa0202030 AT4G16660.2 28.599 521 337 9 6 495 47 563 4.74e-62 221

Find 150 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCCTTTCTTGTCTTCC+TGG 0.182291 2_1:+24005807 Msa0202030:CDS
CGTAATGTTGTGGCCATTTA+AGG 0.183498 2_1:+24004681 Msa0202030:CDS
TGAGGTAGATTCTTTATTTC+AGG 0.194357 2_1:+24005278 Msa0202030:CDS
GCTATTACAAGGGTTGAATT+TGG 0.209837 2_1:+24003612 Msa0202030:five_prime_UTR
AGAAGTTGCCGAGGCATTTA+TGG 0.220210 2_1:+24004810 Msa0202030:CDS
TGCAATAGATCTTGCACTTT+GGG 0.251522 2_1:-24005462 None:intergenic
CATGATTACTCTTAAGTATA+AGG 0.254806 2_1:+24004729 Msa0202030:CDS
TGATTATTCTAATGACATTA+AGG 0.273543 2_1:-24004924 None:intergenic
ATCCCGGCAATGACCTTAAA+TGG 0.282320 2_1:-24004694 None:intergenic
CTCAACCTCCTCAGGGCTCT+TGG 0.286323 2_1:-24005201 None:intergenic
ATAGATGCTGGTGCCATTGC+TGG 0.288873 2_1:+24004901 Msa0202030:CDS
ACAGAAACAGTTTAAGTAAC+AGG 0.293835 2_1:-24006381 None:intergenic
TGTGTCTATCCTTACAATTA+AGG 0.312423 2_1:+24005050 Msa0202030:CDS
ATATCTCCTCAGCACAAAAT+TGG 0.314318 2_1:-24004762 None:intergenic
TTGTCTTCCTGGTGCTCCTC+GGG 0.343237 2_1:+24005818 Msa0202030:CDS
GACACCAAAGCATACATCTA+AGG 0.344681 2_1:-24005847 None:intergenic
TTGTAAGTGATCAAACCTTT+AGG 0.346295 2_1:+24006306 Msa0202030:three_prime_UTR
CCCTTGTAATAGCTATCAAT+TGG 0.351372 2_1:-24003602 None:intergenic
AACCCACAAAACACCATCTT+TGG 0.355538 2_1:+24004112 Msa0202030:CDS
TGGCCATTTAAGGTCATTGC+CGG 0.361953 2_1:+24004691 Msa0202030:CDS
TTCAAGTTGATGATAATGTT+TGG 0.364250 2_1:+24006352 Msa0202030:three_prime_UTR
CTTGTCTTCCTGGTGCTCCT+CGG 0.365730 2_1:+24005817 Msa0202030:CDS
CTGCCGGAAACACTCACCTT+GGG 0.368436 2_1:+24005097 Msa0202030:CDS
CTATTACAAGGGTTGAATTT+GGG 0.370506 2_1:+24003613 Msa0202030:five_prime_UTR
AGCAATGGCACCAGCATCTA+TGG 0.370613 2_1:-24004899 None:intergenic
CCGGACGAAGCTGTTGCTTA+TGG 0.371889 2_1:+24005525 Msa0202030:CDS
TCAACCTCCTCAGGGCTCTT+GGG 0.372118 2_1:-24005200 None:intergenic
ACATCTCGCAACACCAAGTT+CGG 0.374558 2_1:-24005594 None:intergenic
ACTCATGATCAAAGGTTGAT+TGG 0.377170 2_1:+24004061 Msa0202030:CDS
GGAGTTTCGAAAGAAGAATA+AGG 0.381301 2_1:+24005161 Msa0202030:CDS
CATGTTAATTTCCTCAAACT+TGG 0.390891 2_1:-24005331 None:intergenic
ATTGATGATGTTGTTCTTGT+GGG 0.393714 2_1:+24005423 Msa0202030:CDS
TTTCCTTAGCAGAAACAGTT+AGG 0.394977 2_1:-24005884 None:intergenic
CTTATACTTAAGAGTAATCA+TGG 0.395729 2_1:-24004728 None:intergenic
CAACTTCTCGCATCTTTGTA+AGG 0.397847 2_1:-24004795 None:intergenic
TCAGATGCTAAAAGGTTAAT+CGG 0.398046 2_1:+24004625 Msa0202030:intron
AAGAATAAGGTGGACATAAG+TGG 0.400065 2_1:+24005174 Msa0202030:CDS
CTGAAATACCAAGCGACAAT+GGG 0.401504 2_1:-24005620 None:intergenic
AAGGTGAGTGTTTCCGGCAG+TGG 0.402601 2_1:-24005094 None:intergenic
ATGCAGTTCTCAACCTCCTC+AGG 0.403568 2_1:-24005209 None:intergenic
CTTGGTATTTCAGCTGGTGC+TGG 0.410689 2_1:+24005630 Msa0202030:CDS
TGCTTTGGTGTCGATGAAAA+TGG 0.410973 2_1:+24005858 Msa0202030:CDS
TTTAAATTTGGTCTATGCTA+TGG 0.420325 2_1:-24003675 None:intergenic
TTGTCGCTTGGTATTTCAGC+TGG 0.420910 2_1:+24005624 Msa0202030:CDS
GAAGTGATTGTGATGGTAAA+CGG 0.431924 2_1:+24004989 Msa0202030:CDS
TTCTTGTGGGTGGCTCATCT+AGG 0.432668 2_1:+24005436 Msa0202030:CDS
GTGTCTATCCTTACAATTAA+GGG 0.433200 2_1:+24005051 Msa0202030:CDS
CAACCCTTAGATGTATGCTT+TGG 0.435265 2_1:+24005843 Msa0202030:CDS
CCCAATTGATAGCTATTACA+AGG 0.445649 2_1:+24003601 None:intergenic
AAACATCACCCTTAATTGTA+AGG 0.451096 2_1:-24005059 None:intergenic
CCATAAGCAACAGCTTCGTC+CGG 0.455028 2_1:-24005525 None:intergenic
AAGGTGTTGTTCTGTTTCCT+TGG 0.457681 2_1:-24004024 None:intergenic
TTCTGTCTTTGTCACAGGTA+TGG 0.459531 2_1:-24005688 None:intergenic
CTTTAAGAATGTCCCGAACT+TGG 0.464429 2_1:+24005581 Msa0202030:CDS
ATGATTACTCTTAAGTATAA+GGG 0.468173 2_1:+24004730 Msa0202030:CDS
TTGCAATAGATCTTGCACTT+TGG 0.473482 2_1:-24005463 None:intergenic
AATCACCCGTGCCAAGTTTG+AGG 0.476109 2_1:+24005320 Msa0202030:CDS
GATCTTCTCAATTTCTTGAG+AGG 0.476656 2_1:-24006114 None:intergenic
AGTCTCGATTAATGTTTATG+AGG 0.482176 2_1:+24005743 Msa0202030:CDS
GGTTGGCCCCGAGGAGCACC+AGG 0.483124 2_1:-24005825 None:intergenic
AAAATAAAACTAGCCAGTCT+TGG 0.483979 2_1:-24006252 None:intergenic
TTCACAATGGTTATCTTTAG+CGG 0.485360 2_1:-24005721 None:intergenic
ACTGCTGCAGCTATTGCATA+TGG 0.493355 2_1:+24004955 Msa0202030:CDS
AGCACCAGGAAGACAAGAAA+GGG 0.493623 2_1:-24005811 None:intergenic
CGAAAAGCCACCATAGATGC+TGG 0.496808 2_1:+24004889 Msa0202030:CDS
TCATGAGTGTGGTGGTTCCT+AGG 0.497029 2_1:+24005658 Msa0202030:CDS
GAGCACCAGGAAGACAAGAA+AGG 0.498495 2_1:-24005812 None:intergenic
TATTGATGATGTTGTTCTTG+TGG 0.498968 2_1:+24005422 Msa0202030:CDS
GAGGTAGATTCTTTATTTCA+GGG 0.500232 2_1:+24005279 Msa0202030:CDS
TGGTGATGCTGCTAAGAATC+AGG 0.503682 2_1:+24004081 Msa0202030:CDS
ATCCAATGCATTCATTACCT+TGG 0.506126 2_1:-24006036 None:intergenic
TTTGTCTTTGACCTTGGCGG+TGG 0.507107 2_1:+24005018 Msa0202030:CDS
ACTGCCGGAAACACTCACCT+TGG 0.509634 2_1:+24005096 Msa0202030:CDS
AATCGTTCTGTCTTTGTCAC+AGG 0.515077 2_1:-24005693 None:intergenic
GAATGCTGTTGTTACTGTGC+CGG 0.516874 2_1:+24004846 Msa0202030:CDS
GCTACTGACAATAATCTGCT+TGG 0.517838 2_1:+24005780 Msa0202030:CDS
GATGTTACCCCATTGTCGCT+TGG 0.519252 2_1:+24005612 Msa0202030:CDS
TCTGCTAAGGAAATATCTAC+AGG 0.519487 2_1:+24005894 Msa0202030:CDS
TTGCTTTCACTCATGATCAA+AGG 0.522460 2_1:+24004053 Msa0202030:CDS
GAATAGAAACATACCAAAGA+TGG 0.524336 2_1:-24004125 None:intergenic
GTAACAACAGCATTCTTGAC+AGG 0.524961 2_1:-24004838 None:intergenic
GTCTCGATTAATGTTTATGA+GGG 0.526417 2_1:+24005744 Msa0202030:CDS
AAAAGAAAGATTGTCAACCT+TGG 0.531815 2_1:+24005947 Msa0202030:CDS
AGAATAAGGTGGACATAAGT+GGG 0.534398 2_1:+24005175 Msa0202030:CDS
TGAGTCTCATCTGAAGGAGA+TGG 0.534458 2_1:+24006205 Msa0202030:CDS
ACAGGTATGGAAGTGTTCCT+AGG 0.535301 2_1:-24005675 None:intergenic
TGAGTGAAAGCAACAAAAGA+AGG 0.535631 2_1:-24004043 None:intergenic
CATCTCGCAACACCAAGTTC+GGG 0.537564 2_1:-24005593 None:intergenic
ATGCTAAAAGGTTAATCGGT+AGG 0.537744 2_1:+24004629 Msa0202030:CDS
CCAATTGATAGCTATTACAA+GGG 0.538001 2_1:+24003602 None:intergenic
GATAATGACAATCAACCTAA+AGG 0.538448 2_1:-24006321 None:intergenic
TATCCTAACTGTTTCTGCTA+AGG 0.538654 2_1:+24005881 Msa0202030:CDS
TGCGAATCATTGAAGTAAGC+CGG 0.544988 2_1:-24004865 None:intergenic
ATTCTTATCAAGCAAACTTG+TGG 0.546269 2_1:-24006156 None:intergenic
CGTGCAGTGGGAATCGACCT+TGG 0.552866 2_1:+24003935 Msa0202030:CDS
TTCATGTGTTGGTGTGTGGA+TGG 0.555426 2_1:+24003967 Msa0202030:CDS
AAACAACCCCTCGAAAACTT+CGG 0.555908 2_1:+24003640 Msa0202030:five_prime_UTR
CATGGGTTTGTCATTCATCC+CGG 0.556897 2_1:-24004710 None:intergenic
CATAAGCAACAGCTTCGTCC+GGG 0.559975 2_1:-24005524 None:intergenic
GGCCAAGGTAATGAATGCAT+TGG 0.562571 2_1:+24006034 Msa0202030:CDS
GGCCATTTAAGGTCATTGCC+GGG 0.564420 2_1:+24004692 Msa0202030:CDS
AATTTCCTCAAACTTGGCAC+GGG 0.568607 2_1:-24005325 None:intergenic
TGCCGGAAACACTCACCTTG+GGG 0.571947 2_1:+24005098 Msa0202030:CDS
TGCAGTTCTCAACCTCCTCA+GGG 0.574648 2_1:-24005208 None:intergenic
TAATTTCCTCAAACTTGGCA+CGG 0.574799 2_1:-24005326 None:intergenic
TAAAGAATCTACCTCAACAT+TGG 0.576497 2_1:-24005271 None:intergenic
CACATGAATTAGTTGTTCCA+AGG 0.578559 2_1:-24003952 None:intergenic
GATGATGTTGTTCTTGTGGG+TGG 0.585148 2_1:+24005426 Msa0202030:CDS
CAAGTTTGAGGAAATTAACA+TGG 0.586747 2_1:+24005332 Msa0202030:CDS
AAACATGAAGGACGTGCAGT+GGG 0.588952 2_1:+24003923 Msa0202030:CDS
AAAACATGAAGGACGTGCAG+TGG 0.589458 2_1:+24003922 Msa0202030:CDS
GCTGAAATACCAAGCGACAA+TGG 0.589730 2_1:-24005621 None:intergenic
TTCAAAATGACGTAATGTTG+TGG 0.591296 2_1:+24004671 Msa0202030:CDS
GGCTCTTGTGATTGAACGAG+AGG 0.591760 2_1:-24003751 None:intergenic
TTTGAGGTAAAAGCCACTGC+CGG 0.593305 2_1:+24005081 Msa0202030:CDS
GGAACAACTAATTCATGTGT+TGG 0.594307 2_1:+24003956 Msa0202030:CDS
ACACCAAAGCATACATCTAA+GGG 0.595426 2_1:-24005846 None:intergenic
TGTTCTTGAGTCTCATCTGA+AGG 0.596826 2_1:+24006199 Msa0202030:CDS
AGGCGACTCCATAAATGCCT+CGG 0.598734 2_1:-24004818 None:intergenic
GGTTGAGAACTGCATGTGAG+AGG 0.599826 2_1:+24005217 Msa0202030:CDS
AAGCACGTTATAGCCAAGAC+TGG 0.601529 2_1:+24006239 Msa0202030:CDS
GTTGAGAACTGCATGTGAGA+GGG 0.602153 2_1:+24005218 Msa0202030:CDS
AATGACATTAAGGCCAGCAA+TGG 0.607886 2_1:-24004914 None:intergenic
TGTCTTCCTGGTGCTCCTCG+GGG 0.608120 2_1:+24005819 Msa0202030:CDS
TTATACTTAAGAGTAATCAT+GGG 0.609335 2_1:-24004727 None:intergenic
CTAATTCATGTGTTGGTGTG+TGG 0.610145 2_1:+24003963 Msa0202030:CDS
TATGCAATAGCTGCAGCAGT+AGG 0.612017 2_1:-24004952 None:intergenic
AAAATTTCTTATCTTCCATG+TGG 0.619579 2_1:-24006004 None:intergenic
ACATCTAAGGGTTGGCCCCG+AGG 0.632340 2_1:-24005834 None:intergenic
TGCTGGTTGTGTCATGAGTG+TGG 0.633220 2_1:+24005647 Msa0202030:CDS
CAAAGCATACATCTAAGGGT+TGG 0.638080 2_1:-24005842 None:intergenic
CTGCATGTGAGAGGGCAAAG+AGG 0.638103 2_1:+24005226 Msa0202030:CDS
CATCAAAAGTACCACCGCCA+AGG 0.643146 2_1:-24005029 None:intergenic
GTTTCGAAAGAAGAATAAGG+TGG 0.643891 2_1:+24005164 Msa0202030:CDS
AACGGTGAACTACTTTGTAG+AGG 0.645214 2_1:+24005140 Msa0202030:CDS
GAGCCAGCACAAGAAAACAA+AGG 0.647648 2_1:+24003769 Msa0202030:five_prime_UTR
GCCGGAAACACTCACCTTGG+GGG 0.654515 2_1:+24005099 Msa0202030:CDS
GGAACCCAAGAGCCCTGAGG+AGG 0.658456 2_1:+24005196 Msa0202030:CDS
AATGGCACCAGCATCTATGG+TGG 0.660947 2_1:-24004896 None:intergenic
TTTATGCAAGAGTATCAACC+CGG 0.661171 2_1:+24005506 Msa0202030:CDS
AGAAGCTGAGAGATACCACA+TGG 0.679257 2_1:+24005989 Msa0202030:CDS
GATCAACAATGCAATTACTG+TGG 0.689297 2_1:+24006133 Msa0202030:CDS
AAAGATGCGAGAAGTTGCCG+AGG 0.691542 2_1:+24004801 Msa0202030:CDS
GAGATCATACACAATGACCA+AGG 0.693570 2_1:+24004007 Msa0202030:CDS
AGAGGACTTTGATAACAGAA+CGG 0.697041 2_1:+24005122 Msa0202030:CDS
TGAAATACCAAGCGACAATG+GGG 0.703067 2_1:-24005619 None:intergenic
GACAAGAGAAGTGATTGTGA+TGG 0.711617 2_1:+24004982 Msa0202030:CDS
TGGTTGTGTCATGAGTGTGG+TGG 0.717303 2_1:+24005650 Msa0202030:CDS
CATTAATCGAGACTTCACAA+TGG 0.738575 2_1:-24005734 None:intergenic
GTGGGAACCCAAGAGCCCTG+AGG 0.755266 2_1:+24005193 Msa0202030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACATTTCTTAAAT+AGG - chr2_1:24003869-24003888 None:intergenic 10.0%
!!! TAATATATTTTTTTTTTGGT+GGG + chr2_1:24004396-24004415 Msa0202030:intron 10.0%
!!! CAGTTTTTTAGTTATTTTAA+GGG + chr2_1:24004248-24004267 Msa0202030:intron 15.0%
!!! CTAATATATTTTTTTTTTGG+TGG + chr2_1:24004395-24004414 Msa0202030:intron 15.0%
!!! CTAAATTTTATTTAAAACCA+TGG + chr2_1:24004559-24004578 Msa0202030:intron 15.0%
!!! AATCATTTTTTTAATTTCCA+AGG - chr2_1:24005967-24005986 None:intergenic 15.0%
!! TTTGTATACTTGAAAGATTA+TGG + chr2_1:24003895-24003914 Msa0202030:intron 20.0%
!! CAAAACTGTGAATATTTATA+GGG - chr2_1:24004204-24004223 None:intergenic 20.0%
!!! GCAGTTTTTTAGTTATTTTA+AGG + chr2_1:24004247-24004266 Msa0202030:intron 20.0%
!!! TAATAAAACTCATTTTCCAT+CGG - chr2_1:24004305-24004324 None:intergenic 20.0%
!!! GAAAATGAGTTTTATTATCT+GGG + chr2_1:24004308-24004327 Msa0202030:intron 20.0%
!!! ACTACTATTACGATTTTAAA+TGG + chr2_1:24004478-24004497 Msa0202030:intron 20.0%
!! TTATACTTAAGAGTAATCAT+GGG - chr2_1:24004730-24004749 None:intergenic 20.0%
!! ATGATTACTCTTAAGTATAA+GGG + chr2_1:24004730-24004749 Msa0202030:CDS 20.0%
!! TGATTATTCTAATGACATTA+AGG - chr2_1:24004927-24004946 None:intergenic 20.0%
!!! AGATAAGAAATTTTTACAGA+AGG + chr2_1:24006013-24006032 Msa0202030:CDS 20.0%
! TTTAAATTTGGTCTATGCTA+TGG - chr2_1:24003678-24003697 None:intergenic 25.0%
!! GTCTTTCAAGCTTTTAAATT+TGG - chr2_1:24003690-24003709 None:intergenic 25.0%
! ATTATGGCAAAAAAACATGA+AGG + chr2_1:24003911-24003930 Msa0202030:exon 25.0%
! TAACCCTACAACAAATTATT+GGG - chr2_1:24004167-24004186 None:intergenic 25.0%
! TTAACCCTACAACAAATTAT+TGG - chr2_1:24004168-24004187 None:intergenic 25.0%
! GCAAAACTGTGAATATTTAT+AGG - chr2_1:24004205-24004224 None:intergenic 25.0%
!!! GGAAAATGAGTTTTATTATC+TGG + chr2_1:24004307-24004326 Msa0202030:intron 25.0%
!!! AAAATGCAGACACTTATTTT+TGG - chr2_1:24004334-24004353 None:intergenic 25.0%
!! ACTATTACGATTTTAAATGG+CGG + chr2_1:24004481-24004500 Msa0202030:intron 25.0%
! TAATAATTGCATAATAGCCA+TGG - chr2_1:24004579-24004598 None:intergenic 25.0%
!!! TTGTTTTTTCAGATGCTAAA+AGG + chr2_1:24004617-24004636 Msa0202030:intron 25.0%
! CTTATACTTAAGAGTAATCA+TGG - chr2_1:24004731-24004750 None:intergenic 25.0%
! CATGATTACTCTTAAGTATA+AGG + chr2_1:24004729-24004748 Msa0202030:CDS 25.0%
!!! AATATTTTTGTCTTTGACCT+TGG + chr2_1:24005012-24005031 Msa0202030:CDS 25.0%
!!! TCTTTCTTTTTCATTGGTTA+TGG - chr2_1:24005937-24005956 None:intergenic 25.0%
!!! TGACAATCTTTCTTTTTCAT+TGG - chr2_1:24005943-24005962 None:intergenic 25.0%
! AAAATTTCTTATCTTCCATG+TGG - chr2_1:24006007-24006026 None:intergenic 25.0%
!!! TTCAAGTTGATGATAATGTT+TGG + chr2_1:24006352-24006371 Msa0202030:three_prime_UTR 25.0%
CTATTACAAGGGTTGAATTT+GGG + chr2_1:24003613-24003632 Msa0202030:five_prime_UTR 30.0%
GAATAGAAACATACCAAAGA+TGG - chr2_1:24004128-24004147 None:intergenic 30.0%
! ATGTTTCTATTCTCGTATCT+AGG + chr2_1:24004136-24004155 Msa0202030:intron 30.0%
! TGTTTCTATTCTCGTATCTA+GGG + chr2_1:24004137-24004156 Msa0202030:intron 30.0%
!!! AAACTGCGATTTTTTTGCAA+GGG - chr2_1:24004234-24004253 None:intergenic 30.0%
!!! AAAACTGCGATTTTTTTGCA+AGG - chr2_1:24004235-24004254 None:intergenic 30.0%
TAAGGGATCAAAAATGTTGA+TGG + chr2_1:24004265-24004284 Msa0202030:intron 30.0%
!!! TATTTTTTTTTTGGTGGGTG+AGG + chr2_1:24004401-24004420 Msa0202030:intron 30.0%
CAACAACATTAAATACATGC+AGG - chr2_1:24004453-24004472 None:intergenic 30.0%
TCAGATGCTAAAAGGTTAAT+CGG + chr2_1:24004625-24004644 Msa0202030:intron 30.0%
!! ATTACGTCATTTTGAACAAC+AGG - chr2_1:24004667-24004686 None:intergenic 30.0%
TTCAAAATGACGTAATGTTG+TGG + chr2_1:24004671-24004690 Msa0202030:CDS 30.0%
TGTGTCTATCCTTACAATTA+AGG + chr2_1:24005050-24005069 Msa0202030:CDS 30.0%
GTGTCTATCCTTACAATTAA+GGG + chr2_1:24005051-24005070 Msa0202030:CDS 30.0%
AAACATCACCCTTAATTGTA+AGG - chr2_1:24005062-24005081 None:intergenic 30.0%
!! AATTAAGGGTGATGTTTTTG+AGG + chr2_1:24005065-24005084 Msa0202030:CDS 30.0%
TAAAGAATCTACCTCAACAT+TGG - chr2_1:24005274-24005293 None:intergenic 30.0%
! TGAGGTAGATTCTTTATTTC+AGG + chr2_1:24005278-24005297 Msa0202030:CDS 30.0%
! GAGGTAGATTCTTTATTTCA+GGG + chr2_1:24005279-24005298 Msa0202030:CDS 30.0%
CATGTTAATTTCCTCAAACT+TGG - chr2_1:24005334-24005353 None:intergenic 30.0%
CAAGTTTGAGGAAATTAACA+TGG + chr2_1:24005332-24005351 Msa0202030:CDS 30.0%
!! TATTGATGATGTTGTTCTTG+TGG + chr2_1:24005422-24005441 Msa0202030:CDS 30.0%
!! ATTGATGATGTTGTTCTTGT+GGG + chr2_1:24005423-24005442 Msa0202030:CDS 30.0%
!!! TCTATTGCAAGACTTTTTCA+AGG + chr2_1:24005476-24005495 Msa0202030:CDS 30.0%
!!! CTATTGCAAGACTTTTTCAA+GGG + chr2_1:24005477-24005496 Msa0202030:CDS 30.0%
!!! TATTGCAAGACTTTTTCAAG+GGG + chr2_1:24005478-24005497 Msa0202030:CDS 30.0%
TTCACAATGGTTATCTTTAG+CGG - chr2_1:24005724-24005743 None:intergenic 30.0%
AGTCTCGATTAATGTTTATG+AGG + chr2_1:24005743-24005762 Msa0202030:CDS 30.0%
GTCTCGATTAATGTTTATGA+GGG + chr2_1:24005744-24005763 Msa0202030:CDS 30.0%
AAAAGAAAGATTGTCAACCT+TGG + chr2_1:24005947-24005966 Msa0202030:CDS 30.0%
ATTCTTATCAAGCAAACTTG+TGG - chr2_1:24006159-24006178 None:intergenic 30.0%
AAAATAAAACTAGCCAGTCT+TGG - chr2_1:24006255-24006274 None:intergenic 30.0%
TTGTAAGTGATCAAACCTTT+AGG + chr2_1:24006306-24006325 Msa0202030:three_prime_UTR 30.0%
GATAATGACAATCAACCTAA+AGG - chr2_1:24006324-24006343 None:intergenic 30.0%
ACAGAAACAGTTTAAGTAAC+AGG - chr2_1:24006384-24006403 None:intergenic 30.0%
GCTATTACAAGGGTTGAATT+TGG + chr2_1:24003612-24003631 Msa0202030:five_prime_UTR 35.0%
CACATGAATTAGTTGTTCCA+AGG - chr2_1:24003955-24003974 None:intergenic 35.0%
GGAACAACTAATTCATGTGT+TGG + chr2_1:24003956-24003975 Msa0202030:CDS 35.0%
TGAGTGAAAGCAACAAAAGA+AGG - chr2_1:24004046-24004065 None:intergenic 35.0%
TTGCTTTCACTCATGATCAA+AGG + chr2_1:24004053-24004072 Msa0202030:CDS 35.0%
! ACTCATGATCAAAGGTTGAT+TGG + chr2_1:24004061-24004080 Msa0202030:CDS 35.0%
! TACCAAAGATGGTGTTTTGT+GGG - chr2_1:24004117-24004136 None:intergenic 35.0%
! ATACCAAAGATGGTGTTTTG+TGG - chr2_1:24004118-24004137 None:intergenic 35.0%
CCTACAACAAATTATTGGGT+CGG - chr2_1:24004163-24004182 None:intergenic 35.0%
!! CGACCCAATAATTTGTTGTA+GGG + chr2_1:24004161-24004180 Msa0202030:intron 35.0%
! GGAAATTTTGTAAATGCCGA+TGG + chr2_1:24004286-24004305 Msa0202030:intron 35.0%
ATGCTAAAAGGTTAATCGGT+AGG + chr2_1:24004629-24004648 Msa0202030:CDS 35.0%
ATATCTCCTCAGCACAAAAT+TGG - chr2_1:24004765-24004784 None:intergenic 35.0%
GAAGTGATTGTGATGGTAAA+CGG + chr2_1:24004989-24005008 Msa0202030:CDS 35.0%
! ATTTTTGTCTTTGACCTTGG+CGG + chr2_1:24005015-24005034 Msa0202030:CDS 35.0%
! AGAGGACTTTGATAACAGAA+CGG + chr2_1:24005122-24005141 Msa0202030:CDS 35.0%
GGAGTTTCGAAAGAAGAATA+AGG + chr2_1:24005161-24005180 Msa0202030:CDS 35.0%
GTTTCGAAAGAAGAATAAGG+TGG + chr2_1:24005164-24005183 Msa0202030:CDS 35.0%
AAGAATAAGGTGGACATAAG+TGG + chr2_1:24005174-24005193 Msa0202030:CDS 35.0%
AGAATAAGGTGGACATAAGT+GGG + chr2_1:24005175-24005194 Msa0202030:CDS 35.0%
TAATTTCCTCAAACTTGGCA+CGG - chr2_1:24005329-24005348 None:intergenic 35.0%
TGCAATAGATCTTGCACTTT+GGG - chr2_1:24005465-24005484 None:intergenic 35.0%
TTGCAATAGATCTTGCACTT+TGG - chr2_1:24005466-24005485 None:intergenic 35.0%
!! TTTATGCAAGAGTATCAACC+CGG + chr2_1:24005506-24005525 Msa0202030:CDS 35.0%
CATTAATCGAGACTTCACAA+TGG - chr2_1:24005737-24005756 None:intergenic 35.0%
ACACCAAAGCATACATCTAA+GGG - chr2_1:24005849-24005868 None:intergenic 35.0%
!! TATCCTAACTGTTTCTGCTA+AGG + chr2_1:24005881-24005900 Msa0202030:CDS 35.0%
TTTCCTTAGCAGAAACAGTT+AGG - chr2_1:24005887-24005906 None:intergenic 35.0%
TCTGCTAAGGAAATATCTAC+AGG + chr2_1:24005894-24005913 Msa0202030:CDS 35.0%
! GAAATTTTTACAGAAGGCCA+AGG + chr2_1:24006019-24006038 Msa0202030:CDS 35.0%
ATCCAATGCATTCATTACCT+TGG - chr2_1:24006039-24006058 None:intergenic 35.0%
GATCTTCTCAATTTCTTGAG+AGG - chr2_1:24006117-24006136 None:intergenic 35.0%
GATCAACAATGCAATTACTG+TGG + chr2_1:24006133-24006152 Msa0202030:CDS 35.0%
AAACAACCCCTCGAAAACTT+CGG + chr2_1:24003640-24003659 Msa0202030:five_prime_UTR 40.0%
!! TTAAGTCCGAAGTTTTCGAG+GGG - chr2_1:24003649-24003668 None:intergenic 40.0%
! CTTAAGTCCGAAGTTTTCGA+GGG - chr2_1:24003650-24003669 None:intergenic 40.0%
!!! CTACCTTTGTTTTCTTGTGC+TGG - chr2_1:24003775-24003794 None:intergenic 40.0%
! CTAATTCATGTGTTGGTGTG+TGG + chr2_1:24003963-24003982 Msa0202030:CDS 40.0%
GAGATCATACACAATGACCA+AGG + chr2_1:24004007-24004026 Msa0202030:CDS 40.0%
!! AAGGTGTTGTTCTGTTTCCT+TGG - chr2_1:24004027-24004046 None:intergenic 40.0%
AACCCACAAAACACCATCTT+TGG + chr2_1:24004112-24004131 Msa0202030:CDS 40.0%
!! CCGACCCAATAATTTGTTGT+AGG + chr2_1:24004160-24004179 Msa0202030:intron 40.0%
CGTAATGTTGTGGCCATTTA+AGG + chr2_1:24004681-24004700 Msa0202030:CDS 40.0%
CAACTTCTCGCATCTTTGTA+AGG - chr2_1:24004798-24004817 None:intergenic 40.0%
GTAACAACAGCATTCTTGAC+AGG - chr2_1:24004841-24004860 None:intergenic 40.0%
TGCGAATCATTGAAGTAAGC+CGG - chr2_1:24004868-24004887 None:intergenic 40.0%
AATGACATTAAGGCCAGCAA+TGG - chr2_1:24004917-24004936 None:intergenic 40.0%
GACAAGAGAAGTGATTGTGA+TGG + chr2_1:24004982-24005001 Msa0202030:CDS 40.0%
AACGGTGAACTACTTTGTAG+AGG + chr2_1:24005140-24005159 Msa0202030:CDS 40.0%
! TGCTTTTGTCACCAATGTTG+AGG + chr2_1:24005260-24005279 Msa0202030:CDS 40.0%
AATTTCCTCAAACTTGGCAC+GGG - chr2_1:24005328-24005347 None:intergenic 40.0%
!!! CAAGCTGCTATTTTGAGCAA+AGG + chr2_1:24005558-24005577 Msa0202030:CDS 40.0%
! CTTTAAGAATGTCCCGAACT+TGG + chr2_1:24005581-24005600 Msa0202030:CDS 40.0%
TGAAATACCAAGCGACAATG+GGG - chr2_1:24005622-24005641 None:intergenic 40.0%
CTGAAATACCAAGCGACAAT+GGG - chr2_1:24005623-24005642 None:intergenic 40.0%
TTCTGTCTTTGTCACAGGTA+TGG - chr2_1:24005691-24005710 None:intergenic 40.0%
AATCGTTCTGTCTTTGTCAC+AGG - chr2_1:24005696-24005715 None:intergenic 40.0%
GCTACTGACAATAATCTGCT+TGG + chr2_1:24005780-24005799 Msa0202030:CDS 40.0%
CAAAGCATACATCTAAGGGT+TGG - chr2_1:24005845-24005864 None:intergenic 40.0%
CAACCCTTAGATGTATGCTT+TGG + chr2_1:24005843-24005862 Msa0202030:CDS 40.0%
GACACCAAAGCATACATCTA+AGG - chr2_1:24005850-24005869 None:intergenic 40.0%
!! TGCTTTGGTGTCGATGAAAA+TGG + chr2_1:24005858-24005877 Msa0202030:CDS 40.0%
TGTTCTTGAGTCTCATCTGA+AGG + chr2_1:24006199-24006218 Msa0202030:CDS 40.0%
! GCTTAAGTCCGAAGTTTTCG+AGG - chr2_1:24003651-24003670 None:intergenic 45.0%
GAGCCAGCACAAGAAAACAA+AGG + chr2_1:24003769-24003788 Msa0202030:five_prime_UTR 45.0%
AAAACATGAAGGACGTGCAG+TGG + chr2_1:24003922-24003941 Msa0202030:CDS 45.0%
AAACATGAAGGACGTGCAGT+GGG + chr2_1:24003923-24003942 Msa0202030:CDS 45.0%
! TTCATGTGTTGGTGTGTGGA+TGG + chr2_1:24003967-24003986 Msa0202030:CDS 45.0%
! TGGTGATGCTGCTAAGAATC+AGG + chr2_1:24004081-24004100 Msa0202030:CDS 45.0%
!!! GATGGTGTTTTGTGGGTTAG+CGG - chr2_1:24004110-24004129 None:intergenic 45.0%
! TTAGTAGCCTTACCCTGAGT+CGG + chr2_1:24004426-24004445 Msa0202030:intron 45.0%
! TGGCCATTTAAGGTCATTGC+CGG + chr2_1:24004691-24004710 Msa0202030:CDS 45.0%
ATCCCGGCAATGACCTTAAA+TGG - chr2_1:24004697-24004716 None:intergenic 45.0%
CATGGGTTTGTCATTCATCC+CGG - chr2_1:24004713-24004732 None:intergenic 45.0%
! AGAGAGCCAATTTTGTGCTG+AGG + chr2_1:24004756-24004775 Msa0202030:CDS 45.0%
! AGAAGTTGCCGAGGCATTTA+TGG + chr2_1:24004810-24004829 Msa0202030:CDS 45.0%
GAATGCTGTTGTTACTGTGC+CGG + chr2_1:24004846-24004865 Msa0202030:CDS 45.0%
TATGCAATAGCTGCAGCAGT+AGG - chr2_1:24004955-24004974 None:intergenic 45.0%
ACTGCTGCAGCTATTGCATA+TGG + chr2_1:24004955-24004974 Msa0202030:CDS 45.0%
! TTTGAGGTAAAAGCCACTGC+CGG + chr2_1:24005081-24005100 Msa0202030:CDS 45.0%
GTTGAGAACTGCATGTGAGA+GGG + chr2_1:24005218-24005237 Msa0202030:CDS 45.0%
GATGATGTTGTTCTTGTGGG+TGG + chr2_1:24005426-24005445 Msa0202030:CDS 45.0%
!!! GCAAGACTTTTTCAAGGGGA+AGG + chr2_1:24005482-24005501 Msa0202030:CDS 45.0%
ACATCTCGCAACACCAAGTT+CGG - chr2_1:24005597-24005616 None:intergenic 45.0%
GCTGAAATACCAAGCGACAA+TGG - chr2_1:24005624-24005643 None:intergenic 45.0%
! TTGTCGCTTGGTATTTCAGC+TGG + chr2_1:24005624-24005643 Msa0202030:CDS 45.0%
!! ACAGGTATGGAAGTGTTCCT+AGG - chr2_1:24005678-24005697 None:intergenic 45.0%
TTCACCCTTTCTTGTCTTCC+TGG + chr2_1:24005807-24005826 Msa0202030:CDS 45.0%
AGCACCAGGAAGACAAGAAA+GGG - chr2_1:24005814-24005833 None:intergenic 45.0%
AGAAGCTGAGAGATACCACA+TGG + chr2_1:24005989-24006008 Msa0202030:CDS 45.0%
GGCCAAGGTAATGAATGCAT+TGG + chr2_1:24006034-24006053 Msa0202030:CDS 45.0%
TGAGTCTCATCTGAAGGAGA+TGG + chr2_1:24006205-24006224 Msa0202030:CDS 45.0%
AAGCACGTTATAGCCAAGAC+TGG + chr2_1:24006239-24006258 Msa0202030:CDS 45.0%
!!! AATCTAATATATTTTTTTTT+TGG + chr2_1:24004364-24004383 Msa0202030:intron 5.0%
GGCTCTTGTGATTGAACGAG+AGG - chr2_1:24003754-24003773 None:intergenic 50.0%
! ATACATGCAGGACCGACTCA+GGG - chr2_1:24004441-24004460 None:intergenic 50.0%
! AATACATGCAGGACCGACTC+AGG - chr2_1:24004442-24004461 None:intergenic 50.0%
! GGCCATTTAAGGTCATTGCC+GGG + chr2_1:24004692-24004711 Msa0202030:CDS 50.0%
AAAGATGCGAGAAGTTGCCG+AGG + chr2_1:24004801-24004820 Msa0202030:CDS 50.0%
AGGCGACTCCATAAATGCCT+CGG - chr2_1:24004821-24004840 None:intergenic 50.0%
CGAAAAGCCACCATAGATGC+TGG + chr2_1:24004889-24004908 Msa0202030:CDS 50.0%
AATGGCACCAGCATCTATGG+TGG - chr2_1:24004899-24004918 None:intergenic 50.0%
AGCAATGGCACCAGCATCTA+TGG - chr2_1:24004902-24004921 None:intergenic 50.0%
!! ATAGATGCTGGTGCCATTGC+TGG + chr2_1:24004901-24004920 Msa0202030:CDS 50.0%
TTTGTCTTTGACCTTGGCGG+TGG + chr2_1:24005018-24005037 Msa0202030:CDS 50.0%
!! CATCAAAAGTACCACCGCCA+AGG - chr2_1:24005032-24005051 None:intergenic 50.0%
TATCAAAGTCCTCTCCCCCA+AGG - chr2_1:24005116-24005135 None:intergenic 50.0%
TGCAGTTCTCAACCTCCTCA+GGG - chr2_1:24005211-24005230 None:intergenic 50.0%
ATGCAGTTCTCAACCTCCTC+AGG - chr2_1:24005212-24005231 None:intergenic 50.0%
GGTTGAGAACTGCATGTGAG+AGG + chr2_1:24005217-24005236 Msa0202030:CDS 50.0%
AATCACCCGTGCCAAGTTTG+AGG + chr2_1:24005320-24005339 Msa0202030:CDS 50.0%
TTCTTGTGGGTGGCTCATCT+AGG + chr2_1:24005436-24005455 Msa0202030:CDS 50.0%
CATAAGCAACAGCTTCGTCC+GGG - chr2_1:24005527-24005546 None:intergenic 50.0%
CCATAAGCAACAGCTTCGTC+CGG - chr2_1:24005528-24005547 None:intergenic 50.0%
CATCTCGCAACACCAAGTTC+GGG - chr2_1:24005596-24005615 None:intergenic 50.0%
GATGTTACCCCATTGTCGCT+TGG + chr2_1:24005612-24005631 Msa0202030:CDS 50.0%
CTTGGTATTTCAGCTGGTGC+TGG + chr2_1:24005630-24005649 Msa0202030:CDS 50.0%
! TGCTGGTTGTGTCATGAGTG+TGG + chr2_1:24005647-24005666 Msa0202030:CDS 50.0%
TGGTTGTGTCATGAGTGTGG+TGG + chr2_1:24005650-24005669 Msa0202030:CDS 50.0%
TCATGAGTGTGGTGGTTCCT+AGG + chr2_1:24005658-24005677 Msa0202030:CDS 50.0%
GAGCACCAGGAAGACAAGAA+AGG - chr2_1:24005815-24005834 None:intergenic 50.0%
AAGGTGAGTGTTTCCGGCAG+TGG - chr2_1:24005097-24005116 None:intergenic 55.0%
ACTGCCGGAAACACTCACCT+TGG + chr2_1:24005096-24005115 Msa0202030:CDS 55.0%
CTGCCGGAAACACTCACCTT+GGG + chr2_1:24005097-24005116 Msa0202030:CDS 55.0%
TGCCGGAAACACTCACCTTG+GGG + chr2_1:24005098-24005117 Msa0202030:CDS 55.0%
TCCCCCAAGGTGAGTGTTTC+CGG - chr2_1:24005103-24005122 None:intergenic 55.0%
AAACACTCACCTTGGGGGAG+AGG + chr2_1:24005104-24005123 Msa0202030:CDS 55.0%
TCAACCTCCTCAGGGCTCTT+GGG - chr2_1:24005203-24005222 None:intergenic 55.0%
CTGCATGTGAGAGGGCAAAG+AGG + chr2_1:24005226-24005245 Msa0202030:CDS 55.0%
! CCGGACGAAGCTGTTGCTTA+TGG + chr2_1:24005525-24005544 Msa0202030:CDS 55.0%
CTTGTCTTCCTGGTGCTCCT+CGG + chr2_1:24005817-24005836 Msa0202030:CDS 55.0%
TTGTCTTCCTGGTGCTCCTC+GGG + chr2_1:24005818-24005837 Msa0202030:CDS 55.0%
CGTGCAGTGGGAATCGACCT+TGG + chr2_1:24003935-24003954 Msa0202030:CDS 60.0%
! TGCAGGACCGACTCAGGGTA+AGG - chr2_1:24004436-24004455 None:intergenic 60.0%
GCCGGAAACACTCACCTTGG+GGG + chr2_1:24005099-24005118 Msa0202030:CDS 60.0%
CTCAACCTCCTCAGGGCTCT+TGG - chr2_1:24005204-24005223 None:intergenic 60.0%
TGTCTTCCTGGTGCTCCTCG+GGG + chr2_1:24005819-24005838 Msa0202030:CDS 60.0%
!! ACATCTAAGGGTTGGCCCCG+AGG - chr2_1:24005837-24005856 None:intergenic 60.0%
GTGGGAACCCAAGAGCCCTG+AGG + chr2_1:24005193-24005212 Msa0202030:CDS 65.0%
GGAACCCAAGAGCCCTGAGG+AGG + chr2_1:24005196-24005215 Msa0202030:CDS 65.0%
!! GGTTGGCCCCGAGGAGCACC+AGG - chr2_1:24005828-24005847 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 24003605 24006449 24003605 ID=Msa0202030;Name=Msa0202030
chr2_1 mRNA 24003605 24006449 24003605 ID=Msa0202030-mRNA-1;Parent=Msa0202030;Name=Msa0202030-mRNA-1;_AED=0.22;_eAED=0.22;_QI=189|1|1|1|0.5|0.33|3|184|618
chr2_1 exon 24003605 24003790 24003605 ID=Msa0202030-mRNA-1:exon:9285;Parent=Msa0202030-mRNA-1
chr2_1 exon 24003911 24004133 24003911 ID=Msa0202030-mRNA-1:exon:9286;Parent=Msa0202030-mRNA-1
chr2_1 exon 24004629 24006449 24004629 ID=Msa0202030-mRNA-1:exon:9287;Parent=Msa0202030-mRNA-1
chr2_1 five_prime_UTR 24003605 24003790 24003605 ID=Msa0202030-mRNA-1:five_prime_utr;Parent=Msa0202030-mRNA-1
chr2_1 five_prime_UTR 24003911 24003913 24003911 ID=Msa0202030-mRNA-1:five_prime_utr;Parent=Msa0202030-mRNA-1
chr2_1 CDS 24003914 24004133 24003914 ID=Msa0202030-mRNA-1:cds;Parent=Msa0202030-mRNA-1
chr2_1 CDS 24004629 24006265 24004629 ID=Msa0202030-mRNA-1:cds;Parent=Msa0202030-mRNA-1
chr2_1 three_prime_UTR 24006266 24006449 24006266 ID=Msa0202030-mRNA-1:three_prime_utr;Parent=Msa0202030-mRNA-1
Gene Sequence

>Msa0202030

ATGGCAAAAAAACATGAAGGACGTGCAGTGGGAATCGACCTTGGAACAACTAATTCATGTGTTGGTGTGTGGATGGAACATCAGAACAGAGTAGAGATCATACACAATGACCAAGGAAACAGAACAACACCTTCTTTTGTTGCTTTCACTCATGATCAAAGGTTGATTGGTGATGCTGCTAAGAATCAGGTTGCCGCTAACCCACAAAACACCATCTTTGATGCTAAAAGGTTAATCGGTAGGAAGTTTAGTGATCCTGTTGTTCAAAATGACGTAATGTTGTGGCCATTTAAGGTCATTGCCGGGATGAATGACAAACCCATGATTACTCTTAAGTATAAGGGTCAAGAGAGCCAATTTTGTGCTGAGGAGATATCATCTATGATCCTTACAAAGATGCGAGAAGTTGCCGAGGCATTTATGGAGTCGCCTGTCAAGAATGCTGTTGTTACTGTGCCGGCTTACTTCAATGATTCGCAGCGAAAAGCCACCATAGATGCTGGTGCCATTGCTGGCCTTAATGTCATTAGAATAATCAACGAACCTACTGCTGCAGCTATTGCATATGGTCTTGACAAGAGAAGTGATTGTGATGGTAAACGGAATATTTTTGTCTTTGACCTTGGCGGTGGTACTTTTGATGTGTCTATCCTTACAATTAAGGGTGATGTTTTTGAGGTAAAAGCCACTGCCGGAAACACTCACCTTGGGGGAGAGGACTTTGATAACAGAACGGTGAACTACTTTGTAGAGGAGTTTCGAAAGAAGAATAAGGTGGACATAAGTGGGAACCCAAGAGCCCTGAGGAGGTTGAGAACTGCATGTGAGAGGGCAAAGAGGACACTCTCTTTTGCTTTTGTCACCAATGTTGAGGTAGATTCTTTATTTCAGGGAATTGACTTTTCTTCATCAATCACCCGTGCCAAGTTTGAGGAAATTAACATGGATCTTTTCAACGAGTGTATGACAACTGTTGAGAATTGTCTTAGTGATTCAAAGATGCAAATTGCTGATATTGATGATGTTGTTCTTGTGGGTGGCTCATCTAGGATTCCCAAAGTGCAAGATCTATTGCAAGACTTTTTCAAGGGGAAGGATTTATGCAAGAGTATCAACCCGGACGAAGCTGTTGCTTATGGTGCAGCTGTTCAAGCTGCTATTTTGAGCAAAGGCTTTAAGAATGTCCCGAACTTGGTGTTGCGAGATGTTACCCCATTGTCGCTTGGTATTTCAGCTGGTGCTGGTTGTGTCATGAGTGTGGTGGTTCCTAGGAACACTTCCATACCTGTGACAAAGACAGAACGATTTCACACCGCTAAAGATAACCATTGTGAAGTCTCGATTAATGTTTATGAGGGAGAGAGAGCAAAAGCTACTGACAATAATCTGCTTGGTTTTTTCACCCTTTCTTGTCTTCCTGGTGCTCCTCGGGGCCAACCCTTAGATGTATGCTTTGGTGTCGATGAAAATGGTATCCTAACTGTTTCTGCTAAGGAAATATCTACAGGAAATACGAACAAGATCACCATAACCAATGAAAAAGAAAGATTGTCAACCTTGGAAATTAAAAAAATGATTGAAGAAGCTGAGAGATACCACATGGAAGATAAGAAATTTTTACAGAAGGCCAAGGTAATGAATGCATTGGATTATTGTGTTTACGATGTGAAGAATGCTTTAAAGAAAGATGTGAATTTAAAGCTCTCCTCTCAAGAAATTGAGAAGATCAACAATGCAATTACTGTGGCCACAAGTTTGCTTGATAAGAATAACAAACAAAATGAAATAGATGTTCTTGAGTCTCATCTGAAGGAGATGGAGAGCATGTTGAAGCACGTTATAGCCAAGACTGGCTAG
Protein sequence

>Msa0202030

MAKKHEGRAVGIDLGTTNSCVGVWMEHQNRVEIIHNDQGNRTTPSFVAFTHDQRLIGDAAKNQVAANPQNTIFDAKRLIGRKFSDPVVQNDVMLWPFKVIAGMNDKPMITLKYKGQESQFCAEEISSMILTKMREVAEAFMESPVKNAVVTVPAYFNDSQRKATIDAGAIAGLNVIRIINEPTAAAIAYGLDKRSDCDGKRNIFVFDLGGGTFDVSILTIKGDVFEVKATAGNTHLGGEDFDNRTVNYFVEEFRKKNKVDISGNPRALRRLRTACERAKRTLSFAFVTNVEVDSLFQGIDFSSSITRAKFEEINMDLFNECMTTVENCLSDSKMQIADIDDVVLVGGSSRIPKVQDLLQDFFKGKDLCKSINPDEAVAYGAAVQAAILSKGFKNVPNLVLRDVTPLSLGISAGAGCVMSVVVPRNTSIPVTKTERFHTAKDNHCEVSINVYEGERAKATDNNLLGFFTLSCLPGAPRGQPLDVCFGVDENGILTVSAKEISTGNTNKITITNEKERLSTLEIKKMIEEAERYHMEDKKFLQKAKVMNALDYCVYDVKNALKKDVNLKLSSQEIEKINNAITVATSLLDKNNKQNEIDVLESHLKEMESMLKHVIAKTG*