AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0321670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321670 MtrunA17_Chr2g0298851 92.395 618 47 0 1 618 1 618 0.0 1182
Msa0321670 MtrunA17_Chr7g0225671 79.445 613 123 3 6 617 13 623 0.0 1001
Msa0321670 MtrunA17_Chr7g0223851 78.444 617 131 2 1 616 10 625 0.0 999
Msa0321670 MtrunA17_Chr7g0223721 74.798 619 154 2 1 618 1 618 0.0 981
Msa0321670 MtrunA17_Chr4g0040931 74.434 618 156 2 1 617 1 617 0.0 967
Msa0321670 MtrunA17_Chr2g0298861 72.973 629 158 3 1 617 1 629 0.0 953
Msa0321670 MtrunA17_Chr7g0223821 76.898 619 136 5 1 614 1 617 0.0 949
Msa0321670 MtrunA17_Chr7g0223821 61.905 84 29 2 534 614 620 703 5.38e-19 91.3
Msa0321670 MtrunA17_Chr7g0223831 74.960 619 125 3 1 618 1 590 0.0 942
Msa0321670 MtrunA17_Chr7g0221661 73.984 615 157 3 1 614 1 613 0.0 940
Msa0321670 MtrunA17_Chr4g0012541 74.230 617 148 3 1 616 1 607 0.0 935
Msa0321670 MtrunA17_Chr2g0293581 72.903 620 164 4 1 617 1 619 0.0 934
Msa0321670 MtrunA17_Chr7g0215261 75.040 621 116 6 1 618 1 585 0.0 927
Msa0321670 MtrunA17_Chr5g0427901 72.134 628 161 4 1 616 1 626 0.0 921
Msa0321670 MtrunA17_Chr7g0233311 72.698 619 166 3 1 617 1 618 0.0 921
Msa0321670 MtrunA17_Chr7g0223801 72.609 617 164 5 1 616 1 613 0.0 910
Msa0321670 MtrunA17_Chr7g0228281 73.829 619 113 7 1 617 62 633 0.0 909
Msa0321670 MtrunA17_Chr2g0287631 72.213 619 169 3 1 617 1 618 0.0 905
Msa0321670 MtrunA17_Chr7g0217971 70.712 618 153 5 1 617 1 591 0.0 868
Msa0321670 MtrunA17_Chr7g0233301 69.144 619 157 3 1 617 1 587 0.0 864
Msa0321670 MtrunA17_Chr7g0223711 65.210 618 210 5 1 616 1 615 0.0 825
Msa0321670 MtrunA17_Chr8g0390821 63.582 670 173 9 1 616 1 653 0.0 822
Msa0321670 MtrunA17_Chr7g0223751 64.992 617 212 4 1 616 1 614 0.0 819
Msa0321670 MtrunA17_Chr7g0223031 65.353 609 207 4 1 608 1 606 0.0 813
Msa0321670 MtrunA17_Chr8g0350731 65.154 617 211 4 1 616 29 642 0.0 813
Msa0321670 MtrunA17_Chr7g0223731 64.019 617 218 4 1 616 1 614 0.0 810
Msa0321670 MtrunA17_Chr7g0223181 65.267 619 208 6 1 616 1 615 0.0 810
Msa0321670 MtrunA17_Chr7g0223741 67.682 591 187 4 1 589 1 589 0.0 809
Msa0321670 MtrunA17_Chr7g0223781 65.528 615 207 4 1 614 1 611 0.0 807
Msa0321670 MtrunA17_Chr2g0296391 71.834 529 147 2 1 528 1 528 0.0 807
Msa0321670 MtrunA17_Chr7g0223621 63.799 616 219 4 1 615 1 613 0.0 801
Msa0321670 MtrunA17_Chr4g0031321 64.297 619 214 6 1 616 1 615 0.0 800
Msa0321670 MtrunA17_Chr4g0031301 64.297 619 214 6 1 616 1 615 0.0 800
Msa0321670 MtrunA17_Chr7g0262821 63.974 619 216 6 1 616 1 615 0.0 795
Msa0321670 MtrunA17_Chr4g0073741 63.974 619 215 7 1 616 1 614 0.0 793
Msa0321670 MtrunA17_Chr7g0223291 63.651 619 218 6 1 616 1 615 0.0 792
Msa0321670 MtrunA17_Chr7g0223701 63.548 620 215 6 1 611 1 618 0.0 789
Msa0321670 MtrunA17_Chr7g0248131 63.285 621 221 7 1 616 1 619 0.0 783
Msa0321670 MtrunA17_Chr7g0248151 63.489 619 221 5 1 616 1 617 0.0 780
Msa0321670 MtrunA17_Chr7g0223631 63.209 617 209 6 1 616 1 600 0.0 775
Msa0321670 MtrunA17_Chr1g0166551 60.976 615 125 6 6 618 2 503 0.0 726
Msa0321670 MtrunA17_Chr2g0293601 68.462 520 128 3 1 519 1 485 0.0 723
Msa0321670 MtrunA17_Chr2g0331381 67.161 539 114 4 10 548 7 482 0.0 713
Msa0321670 MtrunA17_Chr7g0223541 63.303 545 195 5 74 616 31 572 0.0 694
Msa0321670 MtrunA17_Chr6g0462681 57.095 592 249 4 1 590 1 589 0.0 670
Msa0321670 MtrunA17_Chr7g0223681 63.378 527 189 4 91 616 2 525 0.0 665
Msa0321670 MtrunA17_Chr6g0462601 57.504 593 245 6 1 590 1 589 0.0 660
Msa0321670 MtrunA17_Chr6g0462711 55.519 616 262 8 9 616 10 621 0.0 643
Msa0321670 MtrunA17_Chr2g0293561 79.679 374 76 0 1 374 1 374 0.0 643
Msa0321670 MtrunA17_Chr2g0284681 54.693 618 253 9 5 613 4 603 0.0 635
Msa0321670 MtrunA17_Chr8g0388761 55.932 590 248 8 7 591 36 618 0.0 625
Msa0321670 MtrunA17_Chr6g0462641 55.184 598 256 7 1 590 1 594 0.0 624
Msa0321670 MtrunA17_Chr8g0388671 55.932 590 248 8 7 591 36 618 0.0 622
Msa0321670 MtrunA17_Chr6g0462771 56.441 590 245 7 9 590 9 594 0.0 622
Msa0321670 MtrunA17_Chr6g0462611 55.684 519 225 5 74 590 22 537 0.0 567
Msa0321670 MtrunA17_Chr2g0299921 70.051 394 114 2 3 396 1023 1412 0.0 563
Msa0321670 MtrunA17_Chr2g0299921 67.766 394 122 3 3 395 1407 1796 6.75e-168 523
Msa0321670 MtrunA17_Chr2g0293271 60.959 438 100 3 21 458 43 409 0.0 526
Msa0321670 MtrunA17_Chr2g0299821 58.850 452 170 4 1 438 1 450 0.0 525
Msa0321670 MtrunA17_Chr2g0299821 49.515 103 40 4 444 538 384 482 8.88e-17 83.6
Msa0321670 MtrunA17_Chr2g0299851 67.095 389 124 2 1 389 1030 1414 1.66e-175 535
Msa0321670 MtrunA17_Chr1g0151991 81.443 291 54 0 1 291 1 291 1.67e-175 499
Msa0321670 MtrunA17_Chr2g0299791 66.838 389 125 2 1 389 1034 1418 1.21e-173 531
Msa0321670 MtrunA17_Chr2g0299881 64.541 392 131 3 2 389 1031 1418 2.41e-166 513
Msa0321670 MtrunA17_Chr2g0299881 54.795 73 31 1 180 250 1403 1475 2.41e-16 83.2
Msa0321670 MtrunA17_Chr2g0279901 46.632 579 280 15 10 580 57 614 8.63e-164 484
Msa0321670 MtrunA17_Chr6g0462861 44.623 623 310 12 6 616 6 605 1.23e-160 475
Msa0321670 MtrunA17_Chr7g0245521 78.388 273 59 0 1 273 1 273 1.32e-158 455
Msa0321670 MtrunA17_Chr4g0049501 45.250 579 289 14 10 580 54 612 1.78e-158 470
Msa0321670 MtrunA17_Chr1g0152001 74.754 305 73 4 315 618 1 302 9.22e-158 455
Msa0321670 MtrunA17_Chr7g0223761 62.269 379 98 5 241 617 3 338 3.37e-157 455
Msa0321670 MtrunA17_Chr2g0277021 46.524 561 278 12 8 562 70 614 4.74e-153 457
Msa0321670 MtrunA17_Chr7g0245531 68.051 313 97 3 305 616 2 312 2.20e-140 410
Msa0321670 MtrunA17_Chr1g0188601 79.058 191 40 0 92 282 1 191 3.40e-108 323
Msa0321670 MtrunA17_Chr1g0188611 60.811 296 109 2 322 617 1 289 4.13e-105 319
Msa0321670 MtrunA17_Chr7g0223671 73.016 189 51 0 74 262 8 196 1.69e-100 304
Msa0321670 MtrunA17_Chr6g0462631 50.789 317 113 3 100 415 22 296 4.55e-96 296
Msa0321670 MtrunA17_Chr6g0462751 45.879 364 134 5 60 417 2 308 6.51e-95 294
Msa0321670 MtrunA17_Chr7g0223771 82.237 152 27 0 93 244 1 152 4.94e-89 273
Msa0321670 MtrunA17_Chr5g0394391 34.615 520 308 16 9 510 29 534 6.52e-83 271
Msa0321670 MtrunA17_Chr7g0223861 86.260 131 18 0 78 208 4 134 7.98e-78 243
Msa0321670 MtrunA17_Chr4g0004331 34.091 440 274 7 10 437 4 439 3.49e-76 259
Msa0321670 MtrunA17_Chr4g0046881 80.851 141 24 1 135 275 14 151 1.83e-75 237
Msa0321670 MtrunA17_Chr2g0331901 33.257 439 277 7 10 436 4 438 4.56e-74 254
Msa0321670 MtrunA17_Chr6g0462791 63.636 176 63 1 108 283 1 175 7.45e-74 234
Msa0321670 MtrunA17_Chr5g0444001 32.653 441 288 6 10 445 4 440 9.78e-74 246
Msa0321670 MtrunA17_Chr3g0098761 32.334 467 307 8 10 475 4 462 2.42e-73 250
Msa0321670 MtrunA17_Chr7g0245541 58.911 202 81 2 418 618 1 201 3.29e-73 233
Msa0321670 MtrunA17_Chr4g0064621 34.047 514 295 17 9 492 140 639 1.76e-63 222
Msa0321670 MtrunA17_Chr3g0120101 29.677 465 300 8 12 454 29 488 6.23e-60 215
Msa0321670 MtrunA17_Chr5g0394221 39.249 293 168 5 299 589 69 353 1.46e-55 192
Msa0321670 MtrunA17_Chr6g0481391 68.852 122 35 1 38 159 13 131 9.94e-55 182
Msa0321670 MtrunA17_Chr6g0462701 53.801 171 55 1 100 270 22 168 1.69e-54 183
Msa0321670 MtrunA17_Chr4g0060241 29.412 510 314 15 10 497 21 506 1.63e-52 189
Msa0321670 MtrunA17_Chr7g0223871 64.844 128 44 1 492 618 7 134 4.98e-46 159
Msa0321670 MtrunA17_Chr4g0046871 75.490 102 24 1 337 438 7 107 9.05e-45 154
Msa0321670 MtrunA17_Chr5g0442171 32.424 330 201 9 170 488 1 319 2.50e-43 158
Msa0321670 MtrunA17_Chr6g0462691 52.448 143 66 2 322 463 1 142 1.13e-38 140
Msa0321670 MtrunA17_Chr5g0394231 34.802 227 97 4 74 299 5 181 6.24e-38 139
Msa0321670 MtrunA17_Chr1g0188591 82.192 73 13 0 1 73 1 73 1.11e-35 129
Msa0321670 MtrunA17_Chr7g0223691 78.082 73 16 0 1 73 1 73 2.94e-33 122
Msa0321670 MtrunA17_Chr6g0481381 72.973 74 20 0 176 249 1 74 1.15e-31 121
Msa0321670 MtrunA17_Chr6g0481381 64.583 96 33 1 516 610 75 170 1.87e-31 120
Msa0321670 MtrunA17_Chr7g0223881 69.880 83 24 1 281 363 2 83 6.93e-31 115
Msa0321670 MtrunA17_Chr7g0223791 61.538 91 34 1 482 571 89 179 1.21e-29 115
Msa0321670 MtrunA17_Chr7g0223791 69.136 81 17 3 56 136 18 90 1.49e-27 109
Msa0321670 MtrunA17_Chr6g0462621 67.123 73 22 1 1 73 1 71 2.99e-26 102
Msa0321670 MtrunA17_Chr7g0221651 48.810 84 43 0 215 298 1 84 4.27e-20 85.1
Msa0321670 MtrunA17_Chr6g0462801 59.091 66 22 1 322 382 1 66 6.86e-17 75.9
Msa0321670 MtrunA17_Chr4g0064651 41.935 93 52 2 397 488 29 120 2.63e-16 75.5
Msa0321670 MtrunA17_Chr2g0284661 51.190 84 39 2 368 450 61 143 2.33e-15 73.6
Msa0321670 MtrunA17_Chr7g0223661 38.261 115 27 2 315 429 1 71 4.96e-15 70.9
Msa0321670 MtrunA17_Chr5g0442161 31.373 153 98 4 9 157 28 177 5.38e-15 73.6
Msa0321670 MtrunA17_Chr2g0299891 65.116 43 15 0 315 357 1 43 1.97e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321670 AT3G12580.1 64.620 619 212 6 1 616 1 615 0.0 806
Msa0321670 AT5G02500.1 63.328 619 220 6 1 616 1 615 0.0 792
Msa0321670 AT5G02490.1 63.005 619 222 6 1 616 1 615 0.0 786
Msa0321670 AT3G09440.1 63.489 619 219 6 1 616 1 615 0.0 784
Msa0321670 AT3G09440.4 63.489 619 219 6 1 616 1 615 0.0 784
Msa0321670 AT3G09440.2 63.489 619 219 6 1 616 1 615 0.0 784
Msa0321670 AT3G09440.3 63.489 619 219 6 1 616 1 615 0.0 784
Msa0321670 AT1G56410.1 64.239 618 214 6 1 616 1 613 0.0 784
Msa0321670 AT1G16030.1 62.197 619 226 7 1 616 1 614 0.0 774
Msa0321670 AT5G28540.1 54.486 613 266 9 7 614 35 639 0.0 632
Msa0321670 AT5G42020.1 54.323 613 267 9 7 614 35 639 0.0 629
Msa0321670 AT5G42020.3 54.323 613 267 9 7 614 40 644 0.0 628
Msa0321670 AT1G09080.2 54.120 619 268 9 7 617 40 650 0.0 619
Msa0321670 AT1G09080.1 54.120 619 268 9 7 617 50 660 0.0 618
Msa0321670 AT5G02500.2 73.008 389 103 1 1 389 1 387 0.0 605
Msa0321670 AT5G42020.2 49.673 612 242 10 7 614 35 584 0.0 549
Msa0321670 AT5G09590.1 46.114 579 284 15 10 580 60 618 8.46e-157 466
Msa0321670 AT4G24280.1 45.739 575 289 13 8 576 79 636 2.16e-153 459
Msa0321670 AT5G49910.1 46.702 561 277 12 8 562 79 623 2.36e-153 459
Msa0321670 AT4G37910.1 47.227 559 268 12 10 560 55 594 5.54e-153 457
Msa0321670 AT4G37910.2 47.227 559 268 12 10 560 55 594 5.54e-153 457
Msa0321670 AT1G79930.2 34.091 440 274 6 10 437 4 439 2.36e-78 265
Msa0321670 AT1G79930.1 34.091 440 274 6 10 437 4 439 5.57e-78 264
Msa0321670 AT1G11660.4 33.180 434 280 5 10 437 4 433 1.01e-77 262
Msa0321670 AT1G11660.3 33.180 434 280 5 10 437 4 433 1.01e-77 262
Msa0321670 AT1G11660.1 33.180 434 280 5 10 437 4 433 1.01e-77 262
Msa0321670 AT1G79920.1 33.409 440 277 5 10 437 4 439 2.05e-77 261
Msa0321670 AT1G79920.3 33.409 440 277 5 10 437 4 439 2.60e-77 261
Msa0321670 AT1G79920.2 33.409 440 277 5 10 437 4 439 2.60e-77 261
Msa0321670 AT1G79920.4 33.409 440 277 5 10 437 4 439 2.60e-77 261
Msa0321670 AT2G32120.1 33.204 515 309 13 9 508 30 524 1.64e-76 254
Msa0321670 AT2G32120.2 33.204 515 309 13 9 508 30 524 1.64e-76 254
Msa0321670 AT1G11660.2 33.166 398 258 4 46 437 1 396 1.09e-69 240
Msa0321670 AT4G16660.1 28.599 521 337 9 6 495 23 539 3.14e-62 221
Msa0321670 AT4G16660.2 28.599 521 337 9 6 495 47 563 3.97e-62 221

Find 148 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCCTTTCTTGTCTTCC+TGG 0.182291 2_4:-23200920 Msa0321670:CDS
CGTAATGTTGTGGCCATTTA+AGG 0.183498 2_4:-23202046 Msa0321670:CDS
TGAGGTAGATTCTTTATTTC+AGG 0.194357 2_4:-23201449 Msa0321670:CDS
GCTATTATAAGGGTTGAATT+TGG 0.194653 2_4:-23203116 Msa0321670:five_prime_UTR
AGAAGTTGCCGAGGCATTTA+TGG 0.220210 2_4:-23201917 Msa0321670:CDS
GAGCATTGAAAGCTATTATA+AGG 0.230410 2_4:-23203127 Msa0321670:five_prime_UTR
TGCAATAGATCTTGCACTTT+GGG 0.251522 2_4:+23201265 None:intergenic
CATGATTACTCTTAAGTATA+AGG 0.254806 2_4:-23201998 Msa0321670:CDS
TGATTATTCTAATGACATTA+AGG 0.273543 2_4:+23201803 None:intergenic
ATCCCGGCAATGACCTTAAA+TGG 0.282320 2_4:+23202033 None:intergenic
ATAGATGCTGGTGCCATTGC+TGG 0.288873 2_4:-23201826 Msa0321670:CDS
ACAGAAACAGTTTAAGTAAC+AGG 0.293835 2_4:+23200346 None:intergenic
CTCAACCTCCTCAAGGCTCT+TGG 0.305675 2_4:+23201526 None:intergenic
TGTGTCTATCCTTACAATTA+AGG 0.312423 2_4:-23201677 Msa0321670:CDS
ATATCTCCTCAGCACAAAAT+TGG 0.314318 2_4:+23201965 None:intergenic
TTGTCTTCCTGGTGCTCCTC+GGG 0.343237 2_4:-23200909 Msa0321670:CDS
GACACCAAAGCATACATCTA+AGG 0.344681 2_4:+23200880 None:intergenic
TTGTAAGTGATCAAACCTTT+AGG 0.346295 2_4:-23200421 Msa0321670:three_prime_UTR
AACCCACAAAACACCATCTT+TGG 0.349356 2_4:-23202616 Msa0321670:intron
TGGCCATTTAAGGTCATTGC+CGG 0.361953 2_4:-23202036 Msa0321670:CDS
TTCAAGTTGATGATAATGTT+TGG 0.364250 2_4:-23200375 Msa0321670:three_prime_UTR
CTTGTCTTCCTGGTGCTCCT+CGG 0.365730 2_4:-23200910 Msa0321670:CDS
CTGCCGGAAACACTCACCTT+GGG 0.368436 2_4:-23201630 Msa0321670:CDS
AGCAATGGCACCAGCATCTA+TGG 0.370613 2_4:+23201828 None:intergenic
CCGGACGAAGCTGTTGCTTA+TGG 0.371889 2_4:-23201202 Msa0321670:CDS
ACATCTCGCAACACCAAGTT+CGG 0.374558 2_4:+23201133 None:intergenic
ACTCATGATCAAAGGTTGAT+TGG 0.377170 2_4:-23202667 Msa0321670:CDS
CTATTATAAGGGTTGAATTT+GGG 0.379044 2_4:-23203115 Msa0321670:five_prime_UTR
GGAGTTTCGAAAGAAGAATA+AGG 0.381301 2_4:-23201566 Msa0321670:CDS
TCAACCTCCTCAAGGCTCTT+GGG 0.384888 2_4:+23201527 None:intergenic
CATGTTAATTTCCTCAAACT+TGG 0.390891 2_4:+23201396 None:intergenic
ATTGATGATGTTGTTCTTGT+GGG 0.393714 2_4:-23201304 Msa0321670:CDS
TTTCCTTAGCAGAAACAGTT+AGG 0.394977 2_4:+23200843 None:intergenic
CTTATACTTAAGAGTAATCA+TGG 0.395729 2_4:+23201999 None:intergenic
CAACTTCTCGCATCTTTGTA+AGG 0.397847 2_4:+23201932 None:intergenic
TCAGATGCTAAAAGGTTAAT+CGG 0.398046 2_4:-23202102 Msa0321670:intron
AAGAATAAGGTGGACATAAG+TGG 0.400065 2_4:-23201553 Msa0321670:CDS
CTGAAATACCAAGCGACAAT+GGG 0.401504 2_4:+23201107 None:intergenic
TTTAAATTTGGTTTATGCTA+TGG 0.402438 2_4:+23203053 None:intergenic
AAGGTGAGTGTTTCCGGCAG+TGG 0.402601 2_4:+23201633 None:intergenic
CTTGGTATTTCAGCTGGTGC+TGG 0.410689 2_4:-23201097 Msa0321670:CDS
TGCTTTGGTGTCGATGAAAA+TGG 0.410973 2_4:-23200869 Msa0321670:CDS
TTGTCGCTTGGTATTTCAGC+TGG 0.420910 2_4:-23201103 Msa0321670:CDS
GAAGTGATTGTGATGGTAAA+CGG 0.431924 2_4:-23201738 Msa0321670:CDS
TTCTTGTGGGTGGCTCATCT+AGG 0.432668 2_4:-23201291 Msa0321670:CDS
GTGTCTATCCTTACAATTAA+GGG 0.433200 2_4:-23201676 Msa0321670:CDS
CAACCCTTAGATGTATGCTT+TGG 0.435265 2_4:-23200884 Msa0321670:CDS
AAACATCACCCTTAATTGTA+AGG 0.451096 2_4:+23201668 None:intergenic
CCATAAGCAACAGCTTCGTC+CGG 0.455028 2_4:+23201202 None:intergenic
AAGGTGTTGTTCTGTTTCCT+TGG 0.457681 2_4:+23202704 None:intergenic
TTCTGTCTTTGTCACAGGTA+TGG 0.459531 2_4:+23201039 None:intergenic
GAGCATGTTGAAGCACGTTA+TGG 0.459634 2_4:-23200498 Msa0321670:CDS
AGCATTGAAAGCTATTATAA+GGG 0.462370 2_4:-23203126 Msa0321670:five_prime_UTR
CTTTAAGAATGTCCCGAACT+TGG 0.464429 2_4:-23201146 Msa0321670:CDS
ATGATTACTCTTAAGTATAA+GGG 0.468173 2_4:-23201997 Msa0321670:CDS
TTGCAATAGATCTTGCACTT+TGG 0.473482 2_4:+23201264 None:intergenic
AATCACCCGTGCCAAGTTTG+AGG 0.476109 2_4:-23201407 Msa0321670:CDS
GATCTTCTCAATTTCTTGAG+AGG 0.476656 2_4:+23200613 None:intergenic
AGTCTCGATTAATGTTTATG+AGG 0.482176 2_4:-23200984 Msa0321670:CDS
GGTTGGCCCCGAGGAGCACC+AGG 0.483124 2_4:+23200902 None:intergenic
TTCACAATGGTTATCTTTAG+CGG 0.485360 2_4:+23201006 None:intergenic
ACTGCTGCAGCTATTGCATA+TGG 0.493355 2_4:-23201772 Msa0321670:CDS
AGCACCAGGAAGACAAGAAA+GGG 0.493623 2_4:+23200916 None:intergenic
CGAAAAGCCACCATAGATGC+TGG 0.496808 2_4:-23201838 Msa0321670:CDS
TCATGAGTGTGGTGGTTCCT+AGG 0.497029 2_4:-23201069 Msa0321670:CDS
GAGCACCAGGAAGACAAGAA+AGG 0.498495 2_4:+23200915 None:intergenic
AAAATAAAACTAGCCAGTCT+TGG 0.498554 2_4:+23200475 None:intergenic
TATTGATGATGTTGTTCTTG+TGG 0.498968 2_4:-23201305 Msa0321670:CDS
GAGGTAGATTCTTTATTTCA+GGG 0.500232 2_4:-23201448 Msa0321670:CDS
ATCCAATGCATTCATTACCT+TGG 0.506126 2_4:+23200691 None:intergenic
TTTGTCTTTGACCTTGGCGG+TGG 0.507107 2_4:-23201709 Msa0321670:CDS
ACTGCCGGAAACACTCACCT+TGG 0.509634 2_4:-23201631 Msa0321670:CDS
AATCGTTCTGTCTTTGTCAC+AGG 0.515077 2_4:+23201034 None:intergenic
GAATGCTGTTGTTACTGTGC+CGG 0.516874 2_4:-23201881 Msa0321670:CDS
GCTACTGACAATAATCTGCT+TGG 0.517838 2_4:-23200947 Msa0321670:CDS
GATGTTACCCCATTGTCGCT+TGG 0.519252 2_4:-23201115 Msa0321670:CDS
TCTGCTAAGGAAATATCTAC+AGG 0.519487 2_4:-23200833 Msa0321670:CDS
TTGCTTTCACTCATGATCAA+AGG 0.522460 2_4:-23202675 Msa0321670:CDS
GTAACAACAGCATTCTTGAC+AGG 0.524961 2_4:+23201889 None:intergenic
GTCTCGATTAATGTTTATGA+GGG 0.526417 2_4:-23200983 Msa0321670:CDS
AAAAGAAAGATTGTCAACCT+TGG 0.531815 2_4:-23200780 Msa0321670:CDS
AGAATAAGGTGGACATAAGT+GGG 0.534398 2_4:-23201552 Msa0321670:CDS
TGAGTCTCATCTGAAGGAGA+TGG 0.534458 2_4:-23200522 Msa0321670:CDS
ACAGGTATGGAAGTGTTCCT+AGG 0.535301 2_4:+23201052 None:intergenic
TGAGTGAAAGCAACAAAAGA+AGG 0.535631 2_4:+23202685 None:intergenic
CATCTCGCAACACCAAGTTC+GGG 0.537564 2_4:+23201134 None:intergenic
ATGCTAAAAGGTTAATCGGT+AGG 0.537744 2_4:-23202098 Msa0321670:CDS
GATAATGACAATCAACCTAA+AGG 0.538448 2_4:+23200406 None:intergenic
TATCCTAACTGTTTCTGCTA+AGG 0.538654 2_4:-23200846 Msa0321670:CDS
GAATAGGAACATACCAAAGA+TGG 0.543527 2_4:+23202603 None:intergenic
TGCGAATCATTGAAGTAAGC+CGG 0.544988 2_4:+23201862 None:intergenic
ATTCTTATCAAGCAAACTTG+TGG 0.546269 2_4:+23200571 None:intergenic
AAGCACGTTATGGCCAAGAC+TGG 0.550525 2_4:-23200488 Msa0321670:CDS
CGTGCAGTGGGAATCGACCT+TGG 0.552866 2_4:-23202793 Msa0321670:CDS
TTCATGTGTTGGTGTGTGGA+TGG 0.555426 2_4:-23202761 Msa0321670:CDS
AAACAACCCCTCGAAAACTT+CGG 0.555908 2_4:-23203088 Msa0321670:five_prime_UTR
CATGGGTTTGTCATTCATCC+CGG 0.556897 2_4:+23202017 None:intergenic
CATAAGCAACAGCTTCGTCC+GGG 0.559975 2_4:+23201203 None:intergenic
GGCCAAGGTAATGAATGCAT+TGG 0.562571 2_4:-23200693 Msa0321670:CDS
GGCCATTTAAGGTCATTGCC+GGG 0.564420 2_4:-23202035 Msa0321670:CDS
GATCAACAATGCAATTATTG+TGG 0.566654 2_4:-23200594 Msa0321670:CDS
AATTTCCTCAAACTTGGCAC+GGG 0.568607 2_4:+23201402 None:intergenic
TGCCGGAAACACTCACCTTG+GGG 0.571947 2_4:-23201629 Msa0321670:CDS
TGCAGTTCTCAACCTCCTCA+AGG 0.573614 2_4:+23201519 None:intergenic
TAATTTCCTCAAACTTGGCA+CGG 0.574799 2_4:+23201401 None:intergenic
TAAAGAATCTACCTCAACAT+TGG 0.576497 2_4:+23201456 None:intergenic
CACATGAATTAGTTGTTCCA+AGG 0.578559 2_4:+23202776 None:intergenic
GATGATGTTGTTCTTGTGGG+TGG 0.585148 2_4:-23201301 Msa0321670:CDS
CAAGTTTGAGGAAATTAACA+TGG 0.586747 2_4:-23201395 Msa0321670:CDS
AAACATGAAGGACGTGCAGT+GGG 0.588952 2_4:-23202805 Msa0321670:CDS
AAAACATGAAGGACGTGCAG+TGG 0.589458 2_4:-23202806 Msa0321670:CDS
GCTGAAATACCAAGCGACAA+TGG 0.589730 2_4:+23201106 None:intergenic
TTCAAAATGACGTAATGTTG+TGG 0.591296 2_4:-23202056 Msa0321670:CDS
GGCTCTTGTGATTGAACGAG+AGG 0.591760 2_4:+23202975 None:intergenic
TTTGAGGTAAAAGCCACTGC+CGG 0.593305 2_4:-23201646 Msa0321670:CDS
GGAACAACTAATTCATGTGT+TGG 0.594307 2_4:-23202772 Msa0321670:CDS
ACACCAAAGCATACATCTAA+GGG 0.595426 2_4:+23200881 None:intergenic
TGTTCTTGAGTCTCATCTGA+AGG 0.596826 2_4:-23200528 Msa0321670:CDS
AGGCGACTCCATAAATGCCT+CGG 0.598734 2_4:+23201909 None:intergenic
GGTTGAGAACTGCATGTGAG+AGG 0.599826 2_4:-23201510 Msa0321670:CDS
GTTGAGAACTGCATGTGAGA+GGG 0.602153 2_4:-23201509 Msa0321670:CDS
AATGACATTAAGGCCAGCAA+TGG 0.607886 2_4:+23201813 None:intergenic
TGTCTTCCTGGTGCTCCTCG+GGG 0.608120 2_4:-23200908 Msa0321670:CDS
TTATACTTAAGAGTAATCAT+GGG 0.609335 2_4:+23202000 None:intergenic
CTAATTCATGTGTTGGTGTG+TGG 0.610145 2_4:-23202765 Msa0321670:CDS
TATGCAATAGCTGCAGCAGT+AGG 0.612017 2_4:+23201775 None:intergenic
AAAATTTCTTATCTTCCATG+TGG 0.619579 2_4:+23200723 None:intergenic
ACATCTAAGGGTTGGCCCCG+AGG 0.632340 2_4:+23200893 None:intergenic
TGCTGGTTGTGTCATGAGTG+TGG 0.633220 2_4:-23201080 Msa0321670:CDS
CAAAGCATACATCTAAGGGT+TGG 0.638080 2_4:+23200885 None:intergenic
CTGCATGTGAGAGGGCAAAG+AGG 0.638103 2_4:-23201501 Msa0321670:CDS
CATCAAAAGTACCACCGCCA+AGG 0.643146 2_4:+23201698 None:intergenic
GTTTCGAAAGAAGAATAAGG+TGG 0.643891 2_4:-23201563 Msa0321670:CDS
AACGGTGAACTACTTTGTAG+AGG 0.645214 2_4:-23201587 Msa0321670:CDS
GAGCCAGCACAAGAAAACAA+AGG 0.647648 2_4:-23202957 Msa0321670:intron
GCCGGAAACACTCACCTTGG+GGG 0.654515 2_4:-23201628 Msa0321670:CDS
AATGGCACCAGCATCTATGG+TGG 0.660947 2_4:+23201831 None:intergenic
TTTATGCAAGAGTATCAACC+CGG 0.661171 2_4:-23201221 Msa0321670:CDS
GGAACCCAAGAGCCTTGAGG+AGG 0.669996 2_4:-23201531 Msa0321670:CDS
AGAAGCTGAGAGATACCACA+TGG 0.679257 2_4:-23200738 Msa0321670:CDS
GTGGGAACCCAAGAGCCTTG+AGG 0.680636 2_4:-23201534 Msa0321670:CDS
AAAGATGCGAGAAGTTGCCG+AGG 0.691542 2_4:-23201926 Msa0321670:CDS
GAGATCATACACAATGACCA+AGG 0.693570 2_4:-23202721 Msa0321670:CDS
AGAGGACTTTGATAACAGAA+CGG 0.697041 2_4:-23201605 Msa0321670:CDS
TGAAATACCAAGCGACAATG+GGG 0.703067 2_4:+23201108 None:intergenic
GACAAGAGAAGTGATTGTGA+TGG 0.711617 2_4:-23201745 Msa0321670:CDS
TGGTTGTGTCATGAGTGTGG+TGG 0.717303 2_4:-23201077 Msa0321670:CDS
CATTAATCGAGACTTCACAA+TGG 0.738575 2_4:+23200993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAACATTTCTTAAAT+AGG + chr2_4:23200596-23200615 None:intergenic 10.0%
!!! TAATATATTTTTTTTTTTGG+TGG - chr2_4:23201120-23201139 Msa0321670:CDS 10.0%
!!! AATATATTTTTTTTTTTGGT+GGG - chr2_4:23201121-23201140 Msa0321670:CDS 10.0%
!! CAAAACTATGAATATTTATA+GGG + chr2_4:23200928-23200947 None:intergenic 15.0%
!!! CAGTTTTTTAGTTATTTTAA+GGG - chr2_4:23200972-23200991 Msa0321670:CDS 15.0%
!!! CTAAATTTTATTTAAAACCA+TGG - chr2_4:23201284-23201303 Msa0321670:CDS 15.0%
!!! AATCATTTTTTTAATTTCCA+AGG + chr2_4:23202692-23202711 None:intergenic 15.0%
!!! TTTTTGAATGGATTTCAATT+GGG + chr2_4:23200303-23200322 None:intergenic 20.0%
!!! TTTAAATTTGGTTTATGCTA+TGG + chr2_4:23200402-23200421 None:intergenic 20.0%
!! TTTGTATACTTGAAAGATTA+TGG - chr2_4:23200619-23200638 Msa0321670:CDS 20.0%
!! GCAAAACTATGAATATTTAT+AGG + chr2_4:23200929-23200948 None:intergenic 20.0%
!!! GCAGTTTTTTAGTTATTTTA+AGG - chr2_4:23200971-23200990 Msa0321670:CDS 20.0%
!!! TAATAAAACTCATTTTCCAT+CGG + chr2_4:23201029-23201048 None:intergenic 20.0%
!!! GAAAATGAGTTTTATTATCT+GGG - chr2_4:23201032-23201051 Msa0321670:CDS 20.0%
!!! ACTACTATTACGATTTTAAA+TGG - chr2_4:23201203-23201222 Msa0321670:CDS 20.0%
!! TTATACTTAAGAGTAATCAT+GGG + chr2_4:23201455-23201474 None:intergenic 20.0%
!! ATGATTACTCTTAAGTATAA+GGG - chr2_4:23201455-23201474 Msa0321670:CDS 20.0%
!! TGATTATTCTAATGACATTA+AGG + chr2_4:23201652-23201671 None:intergenic 20.0%
!!! AGATAAGAAATTTTTACAGA+AGG - chr2_4:23202738-23202757 Msa0321670:CDS 20.0%
!!! CTTTTTGAATGGATTTCAAT+TGG + chr2_4:23200304-23200323 None:intergenic 25.0%
!! AGCATTGAAAGCTATTATAA+GGG - chr2_4:23200326-23200345 Msa0321670:three_prime_UTR 25.0%
! CTATTATAAGGGTTGAATTT+GGG - chr2_4:23200337-23200356 Msa0321670:three_prime_UTR 25.0%
!! GTCTTTCAAGCTTTTAAATT+TGG + chr2_4:23200414-23200433 None:intergenic 25.0%
! ATTATGGCAAAAAAACATGA+AGG - chr2_4:23200635-23200654 Msa0321670:CDS 25.0%
! TAACCCTACAACAAATTATT+GGG + chr2_4:23200891-23200910 None:intergenic 25.0%
! TTAACCCTACAACAAATTAT+TGG + chr2_4:23200892-23200911 None:intergenic 25.0%
!!! GGAAAATGAGTTTTATTATC+TGG - chr2_4:23201031-23201050 Msa0321670:CDS 25.0%
!!! AAAATGCAGACACTTATTTT+TGG + chr2_4:23201058-23201077 None:intergenic 25.0%
!! ACTATTACGATTTTAAATGG+CGG - chr2_4:23201206-23201225 Msa0321670:CDS 25.0%
! TAATAATTGCATAATAGCCA+TGG + chr2_4:23201304-23201323 None:intergenic 25.0%
!!! TTGTTTTTTCAGATGCTAAA+AGG - chr2_4:23201342-23201361 Msa0321670:CDS 25.0%
! CTTATACTTAAGAGTAATCA+TGG + chr2_4:23201456-23201475 None:intergenic 25.0%
! CATGATTACTCTTAAGTATA+AGG - chr2_4:23201454-23201473 Msa0321670:CDS 25.0%
!!! AATATTTTTGTCTTTGACCT+TGG - chr2_4:23201737-23201756 Msa0321670:CDS 25.0%
!!! TCTTTCTTTTTCATTGGTTA+TGG + chr2_4:23202662-23202681 None:intergenic 25.0%
!!! TGACAATCTTTCTTTTTCAT+TGG + chr2_4:23202668-23202687 None:intergenic 25.0%
! AAAATTTCTTATCTTCCATG+TGG + chr2_4:23202732-23202751 None:intergenic 25.0%
!!! TTCAAGTTGATGATAATGTT+TGG - chr2_4:23203077-23203096 Msa0321670:five_prime_UTR 25.0%
! TTCAATGCTCTCTTTTTGAA+TGG + chr2_4:23200315-23200334 None:intergenic 30.0%
!! GAGCATTGAAAGCTATTATA+AGG - chr2_4:23200325-23200344 Msa0321670:three_prime_UTR 30.0%
GCTATTATAAGGGTTGAATT+TGG - chr2_4:23200336-23200355 Msa0321670:three_prime_UTR 30.0%
!!! AAACTGCGATTTTTTTGCAA+GGG + chr2_4:23200958-23200977 None:intergenic 30.0%
!!! AAAACTGCGATTTTTTTGCA+AGG + chr2_4:23200959-23200978 None:intergenic 30.0%
TAAGGGATCAAAAATGTTGA+TGG - chr2_4:23200989-23201008 Msa0321670:CDS 30.0%
!!! ATTTTTTTTTTTGGTGGGTG+AGG - chr2_4:23201126-23201145 Msa0321670:CDS 30.0%
CAACAACATTAAATACATGC+AGG + chr2_4:23201178-23201197 None:intergenic 30.0%
TCAGATGCTAAAAGGTTAAT+CGG - chr2_4:23201350-23201369 Msa0321670:CDS 30.0%
!! ATTACGTCATTTTGAACAAC+AGG + chr2_4:23201392-23201411 None:intergenic 30.0%
TTCAAAATGACGTAATGTTG+TGG - chr2_4:23201396-23201415 Msa0321670:CDS 30.0%
TGTGTCTATCCTTACAATTA+AGG - chr2_4:23201775-23201794 Msa0321670:CDS 30.0%
GTGTCTATCCTTACAATTAA+GGG - chr2_4:23201776-23201795 Msa0321670:CDS 30.0%
AAACATCACCCTTAATTGTA+AGG + chr2_4:23201787-23201806 None:intergenic 30.0%
!! AATTAAGGGTGATGTTTTTG+AGG - chr2_4:23201790-23201809 Msa0321670:CDS 30.0%
TAAAGAATCTACCTCAACAT+TGG + chr2_4:23201999-23202018 None:intergenic 30.0%
! TGAGGTAGATTCTTTATTTC+AGG - chr2_4:23202003-23202022 Msa0321670:CDS 30.0%
! GAGGTAGATTCTTTATTTCA+GGG - chr2_4:23202004-23202023 Msa0321670:CDS 30.0%
CATGTTAATTTCCTCAAACT+TGG + chr2_4:23202059-23202078 None:intergenic 30.0%
CAAGTTTGAGGAAATTAACA+TGG - chr2_4:23202057-23202076 Msa0321670:CDS 30.0%
!! TATTGATGATGTTGTTCTTG+TGG - chr2_4:23202147-23202166 Msa0321670:intron 30.0%
!! ATTGATGATGTTGTTCTTGT+GGG - chr2_4:23202148-23202167 Msa0321670:intron 30.0%
!!! TCTATTGCAAGACTTTTTCA+AGG - chr2_4:23202201-23202220 Msa0321670:intron 30.0%
!!! CTATTGCAAGACTTTTTCAA+GGG - chr2_4:23202202-23202221 Msa0321670:intron 30.0%
!!! TATTGCAAGACTTTTTCAAG+GGG - chr2_4:23202203-23202222 Msa0321670:intron 30.0%
TTCACAATGGTTATCTTTAG+CGG + chr2_4:23202449-23202468 None:intergenic 30.0%
AGTCTCGATTAATGTTTATG+AGG - chr2_4:23202468-23202487 Msa0321670:intron 30.0%
GTCTCGATTAATGTTTATGA+GGG - chr2_4:23202469-23202488 Msa0321670:intron 30.0%
AAAAGAAAGATTGTCAACCT+TGG - chr2_4:23202672-23202691 Msa0321670:CDS 30.0%
GATCAACAATGCAATTATTG+TGG - chr2_4:23202858-23202877 Msa0321670:intron 30.0%
ATTCTTATCAAGCAAACTTG+TGG + chr2_4:23202884-23202903 None:intergenic 30.0%
AAAATAAAACTAGCCAGTCT+TGG + chr2_4:23202980-23202999 None:intergenic 30.0%
TTGTAAGTGATCAAACCTTT+AGG - chr2_4:23203031-23203050 Msa0321670:five_prime_UTR 30.0%
GATAATGACAATCAACCTAA+AGG + chr2_4:23203049-23203068 None:intergenic 30.0%
ACAGAAACAGTTTAAGTAAC+AGG + chr2_4:23203109-23203128 None:intergenic 30.0%
CACATGAATTAGTTGTTCCA+AGG + chr2_4:23200679-23200698 None:intergenic 35.0%
GGAACAACTAATTCATGTGT+TGG - chr2_4:23200680-23200699 Msa0321670:CDS 35.0%
TGAGTGAAAGCAACAAAAGA+AGG + chr2_4:23200770-23200789 None:intergenic 35.0%
TTGCTTTCACTCATGATCAA+AGG - chr2_4:23200777-23200796 Msa0321670:CDS 35.0%
! ACTCATGATCAAAGGTTGAT+TGG - chr2_4:23200785-23200804 Msa0321670:CDS 35.0%
! TACCAAAGATGGTGTTTTGT+GGG + chr2_4:23200841-23200860 None:intergenic 35.0%
! ATACCAAAGATGGTGTTTTG+TGG + chr2_4:23200842-23200861 None:intergenic 35.0%
GAATAGGAACATACCAAAGA+TGG + chr2_4:23200852-23200871 None:intergenic 35.0%
ATGTTCCTATTCTCGTATCT+AGG - chr2_4:23200860-23200879 Msa0321670:CDS 35.0%
CCTACAACAAATTATTGGGT+CGG + chr2_4:23200887-23200906 None:intergenic 35.0%
!! CGACCCAATAATTTGTTGTA+GGG - chr2_4:23200885-23200904 Msa0321670:CDS 35.0%
! GGAAATTTTGTAAATGCCGA+TGG - chr2_4:23201010-23201029 Msa0321670:CDS 35.0%
ATGCTAAAAGGTTAATCGGT+AGG - chr2_4:23201354-23201373 Msa0321670:CDS 35.0%
ATATCTCCTCAGCACAAAAT+TGG + chr2_4:23201490-23201509 None:intergenic 35.0%
GAAGTGATTGTGATGGTAAA+CGG - chr2_4:23201714-23201733 Msa0321670:CDS 35.0%
! ATTTTTGTCTTTGACCTTGG+CGG - chr2_4:23201740-23201759 Msa0321670:CDS 35.0%
! AGAGGACTTTGATAACAGAA+CGG - chr2_4:23201847-23201866 Msa0321670:CDS 35.0%
GGAGTTTCGAAAGAAGAATA+AGG - chr2_4:23201886-23201905 Msa0321670:CDS 35.0%
GTTTCGAAAGAAGAATAAGG+TGG - chr2_4:23201889-23201908 Msa0321670:CDS 35.0%
AAGAATAAGGTGGACATAAG+TGG - chr2_4:23201899-23201918 Msa0321670:CDS 35.0%
AGAATAAGGTGGACATAAGT+GGG - chr2_4:23201900-23201919 Msa0321670:CDS 35.0%
TAATTTCCTCAAACTTGGCA+CGG + chr2_4:23202054-23202073 None:intergenic 35.0%
TGCAATAGATCTTGCACTTT+GGG + chr2_4:23202190-23202209 None:intergenic 35.0%
TTGCAATAGATCTTGCACTT+TGG + chr2_4:23202191-23202210 None:intergenic 35.0%
!! TTTATGCAAGAGTATCAACC+CGG - chr2_4:23202231-23202250 Msa0321670:intron 35.0%
CATTAATCGAGACTTCACAA+TGG + chr2_4:23202462-23202481 None:intergenic 35.0%
ACACCAAAGCATACATCTAA+GGG + chr2_4:23202574-23202593 None:intergenic 35.0%
!! TATCCTAACTGTTTCTGCTA+AGG - chr2_4:23202606-23202625 Msa0321670:intron 35.0%
TTTCCTTAGCAGAAACAGTT+AGG + chr2_4:23202612-23202631 None:intergenic 35.0%
TCTGCTAAGGAAATATCTAC+AGG - chr2_4:23202619-23202638 Msa0321670:CDS 35.0%
! GAAATTTTTACAGAAGGCCA+AGG - chr2_4:23202744-23202763 Msa0321670:CDS 35.0%
ATCCAATGCATTCATTACCT+TGG + chr2_4:23202764-23202783 None:intergenic 35.0%
GATCTTCTCAATTTCTTGAG+AGG + chr2_4:23202842-23202861 None:intergenic 35.0%
AAACAACCCCTCGAAAACTT+CGG - chr2_4:23200364-23200383 Msa0321670:three_prime_UTR 40.0%
!! TTAAGTCCGAAGTTTTCGAG+GGG + chr2_4:23200373-23200392 None:intergenic 40.0%
! CTTAAGTCCGAAGTTTTCGA+GGG + chr2_4:23200374-23200393 None:intergenic 40.0%
!!! CTACCTTTGTTTTCTTGTGC+TGG + chr2_4:23200501-23200520 None:intergenic 40.0%
! CTAATTCATGTGTTGGTGTG+TGG - chr2_4:23200687-23200706 Msa0321670:CDS 40.0%
GAGATCATACACAATGACCA+AGG - chr2_4:23200731-23200750 Msa0321670:CDS 40.0%
!! AAGGTGTTGTTCTGTTTCCT+TGG + chr2_4:23200751-23200770 None:intergenic 40.0%
AACCCACAAAACACCATCTT+TGG - chr2_4:23200836-23200855 Msa0321670:CDS 40.0%
!! CCGACCCAATAATTTGTTGT+AGG - chr2_4:23200884-23200903 Msa0321670:CDS 40.0%
CGTAATGTTGTGGCCATTTA+AGG - chr2_4:23201406-23201425 Msa0321670:CDS 40.0%
CAACTTCTCGCATCTTTGTA+AGG + chr2_4:23201523-23201542 None:intergenic 40.0%
GTAACAACAGCATTCTTGAC+AGG + chr2_4:23201566-23201585 None:intergenic 40.0%
TGCGAATCATTGAAGTAAGC+CGG + chr2_4:23201593-23201612 None:intergenic 40.0%
AATGACATTAAGGCCAGCAA+TGG + chr2_4:23201642-23201661 None:intergenic 40.0%
GACAAGAGAAGTGATTGTGA+TGG - chr2_4:23201707-23201726 Msa0321670:CDS 40.0%
AACGGTGAACTACTTTGTAG+AGG - chr2_4:23201865-23201884 Msa0321670:CDS 40.0%
! TGCTTTTGTCACCAATGTTG+AGG - chr2_4:23201985-23202004 Msa0321670:CDS 40.0%
AATTTCCTCAAACTTGGCAC+GGG + chr2_4:23202053-23202072 None:intergenic 40.0%
!!! CAAGCTGCTATTTTGAGCAA+AGG - chr2_4:23202283-23202302 Msa0321670:intron 40.0%
! CTTTAAGAATGTCCCGAACT+TGG - chr2_4:23202306-23202325 Msa0321670:intron 40.0%
TGAAATACCAAGCGACAATG+GGG + chr2_4:23202347-23202366 None:intergenic 40.0%
CTGAAATACCAAGCGACAAT+GGG + chr2_4:23202348-23202367 None:intergenic 40.0%
TTCTGTCTTTGTCACAGGTA+TGG + chr2_4:23202416-23202435 None:intergenic 40.0%
AATCGTTCTGTCTTTGTCAC+AGG + chr2_4:23202421-23202440 None:intergenic 40.0%
GCTACTGACAATAATCTGCT+TGG - chr2_4:23202505-23202524 Msa0321670:intron 40.0%
CAAAGCATACATCTAAGGGT+TGG + chr2_4:23202570-23202589 None:intergenic 40.0%
CAACCCTTAGATGTATGCTT+TGG - chr2_4:23202568-23202587 Msa0321670:intron 40.0%
GACACCAAAGCATACATCTA+AGG + chr2_4:23202575-23202594 None:intergenic 40.0%
!! TGCTTTGGTGTCGATGAAAA+TGG - chr2_4:23202583-23202602 Msa0321670:intron 40.0%
TGTTCTTGAGTCTCATCTGA+AGG - chr2_4:23202924-23202943 Msa0321670:intron 40.0%
! GCTTAAGTCCGAAGTTTTCG+AGG + chr2_4:23200375-23200394 None:intergenic 45.0%
GAGCCAGCACAAGAAAACAA+AGG - chr2_4:23200495-23200514 Msa0321670:CDS 45.0%
AAAACATGAAGGACGTGCAG+TGG - chr2_4:23200646-23200665 Msa0321670:CDS 45.0%
AAACATGAAGGACGTGCAGT+GGG - chr2_4:23200647-23200666 Msa0321670:CDS 45.0%
! TTCATGTGTTGGTGTGTGGA+TGG - chr2_4:23200691-23200710 Msa0321670:CDS 45.0%
!!! GATGGTGTTTTGTGGGTTAG+AGG + chr2_4:23200834-23200853 None:intergenic 45.0%
TCGGTCCTAGATACGAGAAT+AGG + chr2_4:23200868-23200887 None:intergenic 45.0%
! TTAGTAGCCTTACCCTGAGT+CGG - chr2_4:23201151-23201170 Msa0321670:CDS 45.0%
! TGGCCATTTAAGGTCATTGC+CGG - chr2_4:23201416-23201435 Msa0321670:CDS 45.0%
ATCCCGGCAATGACCTTAAA+TGG + chr2_4:23201422-23201441 None:intergenic 45.0%
CATGGGTTTGTCATTCATCC+CGG + chr2_4:23201438-23201457 None:intergenic 45.0%
! AGAGAGCCAATTTTGTGCTG+AGG - chr2_4:23201481-23201500 Msa0321670:CDS 45.0%
! AGAAGTTGCCGAGGCATTTA+TGG - chr2_4:23201535-23201554 Msa0321670:CDS 45.0%
GAATGCTGTTGTTACTGTGC+CGG - chr2_4:23201571-23201590 Msa0321670:CDS 45.0%
TATGCAATAGCTGCAGCAGT+AGG + chr2_4:23201680-23201699 None:intergenic 45.0%
ACTGCTGCAGCTATTGCATA+TGG - chr2_4:23201680-23201699 Msa0321670:CDS 45.0%
! TTTGAGGTAAAAGCCACTGC+CGG - chr2_4:23201806-23201825 Msa0321670:CDS 45.0%
GTTGAGAACTGCATGTGAGA+GGG - chr2_4:23201943-23201962 Msa0321670:CDS 45.0%
GATGATGTTGTTCTTGTGGG+TGG - chr2_4:23202151-23202170 Msa0321670:intron 45.0%
!!! GCAAGACTTTTTCAAGGGGA+AGG - chr2_4:23202207-23202226 Msa0321670:intron 45.0%
ACATCTCGCAACACCAAGTT+CGG + chr2_4:23202322-23202341 None:intergenic 45.0%
GCTGAAATACCAAGCGACAA+TGG + chr2_4:23202349-23202368 None:intergenic 45.0%
! TTGTCGCTTGGTATTTCAGC+TGG - chr2_4:23202349-23202368 Msa0321670:intron 45.0%
!! ACAGGTATGGAAGTGTTCCT+AGG + chr2_4:23202403-23202422 None:intergenic 45.0%
TTCACCCTTTCTTGTCTTCC+TGG - chr2_4:23202532-23202551 Msa0321670:intron 45.0%
AGCACCAGGAAGACAAGAAA+GGG + chr2_4:23202539-23202558 None:intergenic 45.0%
AGAAGCTGAGAGATACCACA+TGG - chr2_4:23202714-23202733 Msa0321670:CDS 45.0%
GGCCAAGGTAATGAATGCAT+TGG - chr2_4:23202759-23202778 Msa0321670:CDS 45.0%
TGAGTCTCATCTGAAGGAGA+TGG - chr2_4:23202930-23202949 Msa0321670:intron 45.0%
GAGCATGTTGAAGCACGTTA+TGG - chr2_4:23202954-23202973 Msa0321670:intron 45.0%
!!! ATCTAATATATTTTTTTTTT+TGG - chr2_4:23201117-23201136 Msa0321670:CDS 5.0%
GGCTCTTGTGATTGAACGAG+AGG + chr2_4:23200480-23200499 None:intergenic 50.0%
! ATACATGCAGGACCGACTCA+GGG + chr2_4:23201166-23201185 None:intergenic 50.0%
! AATACATGCAGGACCGACTC+AGG + chr2_4:23201167-23201186 None:intergenic 50.0%
! GGCCATTTAAGGTCATTGCC+GGG - chr2_4:23201417-23201436 Msa0321670:CDS 50.0%
AAAGATGCGAGAAGTTGCCG+AGG - chr2_4:23201526-23201545 Msa0321670:CDS 50.0%
AGGCGACTCCATAAATGCCT+CGG + chr2_4:23201546-23201565 None:intergenic 50.0%
CGAAAAGCCACCATAGATGC+TGG - chr2_4:23201614-23201633 Msa0321670:CDS 50.0%
AATGGCACCAGCATCTATGG+TGG + chr2_4:23201624-23201643 None:intergenic 50.0%
AGCAATGGCACCAGCATCTA+TGG + chr2_4:23201627-23201646 None:intergenic 50.0%
!! ATAGATGCTGGTGCCATTGC+TGG - chr2_4:23201626-23201645 Msa0321670:CDS 50.0%
TTTGTCTTTGACCTTGGCGG+TGG - chr2_4:23201743-23201762 Msa0321670:CDS 50.0%
!! CATCAAAAGTACCACCGCCA+AGG + chr2_4:23201757-23201776 None:intergenic 50.0%
TATCAAAGTCCTCTCCCCCA+AGG + chr2_4:23201841-23201860 None:intergenic 50.0%
TCAACCTCCTCAAGGCTCTT+GGG + chr2_4:23201928-23201947 None:intergenic 50.0%
TGCAGTTCTCAACCTCCTCA+AGG + chr2_4:23201936-23201955 None:intergenic 50.0%
GGTTGAGAACTGCATGTGAG+AGG - chr2_4:23201942-23201961 Msa0321670:CDS 50.0%
AATCACCCGTGCCAAGTTTG+AGG - chr2_4:23202045-23202064 Msa0321670:CDS 50.0%
TTCTTGTGGGTGGCTCATCT+AGG - chr2_4:23202161-23202180 Msa0321670:intron 50.0%
CATAAGCAACAGCTTCGTCC+GGG + chr2_4:23202252-23202271 None:intergenic 50.0%
CCATAAGCAACAGCTTCGTC+CGG + chr2_4:23202253-23202272 None:intergenic 50.0%
CATCTCGCAACACCAAGTTC+GGG + chr2_4:23202321-23202340 None:intergenic 50.0%
GATGTTACCCCATTGTCGCT+TGG - chr2_4:23202337-23202356 Msa0321670:intron 50.0%
CTTGGTATTTCAGCTGGTGC+TGG - chr2_4:23202355-23202374 Msa0321670:intron 50.0%
! TGCTGGTTGTGTCATGAGTG+TGG - chr2_4:23202372-23202391 Msa0321670:intron 50.0%
TGGTTGTGTCATGAGTGTGG+TGG - chr2_4:23202375-23202394 Msa0321670:intron 50.0%
TCATGAGTGTGGTGGTTCCT+AGG - chr2_4:23202383-23202402 Msa0321670:intron 50.0%
GAGCACCAGGAAGACAAGAA+AGG + chr2_4:23202540-23202559 None:intergenic 50.0%
AAGCACGTTATGGCCAAGAC+TGG - chr2_4:23202964-23202983 Msa0321670:five_prime_UTR 50.0%
AAGGTGAGTGTTTCCGGCAG+TGG + chr2_4:23201822-23201841 None:intergenic 55.0%
ACTGCCGGAAACACTCACCT+TGG - chr2_4:23201821-23201840 Msa0321670:CDS 55.0%
CTGCCGGAAACACTCACCTT+GGG - chr2_4:23201822-23201841 Msa0321670:CDS 55.0%
TGCCGGAAACACTCACCTTG+GGG - chr2_4:23201823-23201842 Msa0321670:CDS 55.0%
TCCCCCAAGGTGAGTGTTTC+CGG + chr2_4:23201828-23201847 None:intergenic 55.0%
AAACACTCACCTTGGGGGAG+AGG - chr2_4:23201829-23201848 Msa0321670:CDS 55.0%
CTCAACCTCCTCAAGGCTCT+TGG + chr2_4:23201929-23201948 None:intergenic 55.0%
CTGCATGTGAGAGGGCAAAG+AGG - chr2_4:23201951-23201970 Msa0321670:CDS 55.0%
! CCGGACGAAGCTGTTGCTTA+TGG - chr2_4:23202250-23202269 Msa0321670:intron 55.0%
CTTGTCTTCCTGGTGCTCCT+CGG - chr2_4:23202542-23202561 Msa0321670:intron 55.0%
TTGTCTTCCTGGTGCTCCTC+GGG - chr2_4:23202543-23202562 Msa0321670:intron 55.0%
CGTGCAGTGGGAATCGACCT+TGG - chr2_4:23200659-23200678 Msa0321670:CDS 60.0%
! TGCAGGACCGACTCAGGGTA+AGG + chr2_4:23201161-23201180 None:intergenic 60.0%
GCCGGAAACACTCACCTTGG+GGG - chr2_4:23201824-23201843 Msa0321670:CDS 60.0%
GTGGGAACCCAAGAGCCTTG+AGG - chr2_4:23201918-23201937 Msa0321670:CDS 60.0%
GGAACCCAAGAGCCTTGAGG+AGG - chr2_4:23201921-23201940 Msa0321670:CDS 60.0%
TGTCTTCCTGGTGCTCCTCG+GGG - chr2_4:23202544-23202563 Msa0321670:intron 60.0%
!! ACATCTAAGGGTTGGCCCCG+AGG + chr2_4:23202562-23202581 None:intergenic 60.0%
!! GGTTGGCCCCGAGGAGCACC+AGG + chr2_4:23202553-23202572 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 23200300 23203174 23200300 ID=Msa0321670;Name=Msa0321670
chr2_4 mRNA 23200300 23203174 23200300 ID=Msa0321670-mRNA-1;Parent=Msa0321670;Name=Msa0321670-mRNA-1;_AED=0.24;_eAED=0.24;_QI=220|1|1|1|0.5|0.33|3|184|618
chr2_4 exon 23200300 23202120 23200300 ID=Msa0321670-mRNA-1:exon:8538;Parent=Msa0321670-mRNA-1
chr2_4 exon 23202617 23202839 23202617 ID=Msa0321670-mRNA-1:exon:8537;Parent=Msa0321670-mRNA-1
chr2_4 exon 23202958 23203174 23202958 ID=Msa0321670-mRNA-1:exon:8536;Parent=Msa0321670-mRNA-1
chr2_4 five_prime_UTR 23202958 23203174 23202958 ID=Msa0321670-mRNA-1:five_prime_utr;Parent=Msa0321670-mRNA-1
chr2_4 five_prime_UTR 23202837 23202839 23202837 ID=Msa0321670-mRNA-1:five_prime_utr;Parent=Msa0321670-mRNA-1
chr2_4 CDS 23202617 23202836 23202617 ID=Msa0321670-mRNA-1:cds;Parent=Msa0321670-mRNA-1
chr2_4 CDS 23200484 23202120 23200484 ID=Msa0321670-mRNA-1:cds;Parent=Msa0321670-mRNA-1
chr2_4 three_prime_UTR 23200300 23200483 23200300 ID=Msa0321670-mRNA-1:three_prime_utr;Parent=Msa0321670-mRNA-1
Gene Sequence

>Msa0321670

ATGGCAAAAAAACATGAAGGACGTGCAGTGGGAATCGACCTTGGAACAACTAATTCATGTGTTGGTGTGTGGATGGAACATCAGAACAGAGTAGAGATCATACACAATGACCAAGGAAACAGAACAACACCTTCTTTTGTTGCTTTCACTCATGATCAAAGGTTGATTGGTGATGCTGCTAAGAATCAAGTTGCCTCTAACCCACAAAACACCATCTTTGATGCTAAAAGGTTAATCGGTAGGAAGTTTAGTGATCCTGTTGTTCAAAATGACGTAATGTTGTGGCCATTTAAGGTCATTGCCGGGATGAATGACAAACCCATGATTACTCTTAAGTATAAGGGTCAAGAGAGCCAATTTTGTGCTGAGGAGATATCATCTATGATCCTTACAAAGATGCGAGAAGTTGCCGAGGCATTTATGGAGTCGCCTGTCAAGAATGCTGTTGTTACTGTGCCGGCTTACTTCAATGATTCGCAGCGAAAAGCCACCATAGATGCTGGTGCCATTGCTGGCCTTAATGTCATTAGAATAATCAACGAACCTACTGCTGCAGCTATTGCATATGGTCTTGACAAGAGAAGTGATTGTGATGGTAAACGGAATATTTTTGTCTTTGACCTTGGCGGTGGTACTTTTGATGTGTCTATCCTTACAATTAAGGGTGATGTTTTTGAGGTAAAAGCCACTGCCGGAAACACTCACCTTGGGGGAGAGGACTTTGATAACAGAACGGTGAACTACTTTGTAGAGGAGTTTCGAAAGAAGAATAAGGTGGACATAAGTGGGAACCCAAGAGCCTTGAGGAGGTTGAGAACTGCATGTGAGAGGGCAAAGAGGACACTCTCTTTTGCTTTTGTCACCAATGTTGAGGTAGATTCTTTATTTCAGGGAATTGACTTTTCTTCATCAATCACCCGTGCCAAGTTTGAGGAAATTAACATGGATCTTTTCAACGAGTGTATGACAACTGTTGAGAATTGTCTTAGTGATTCAAAGATGCAAATTGCTGATATTGATGATGTTGTTCTTGTGGGTGGCTCATCTAGGATTCCCAAAGTGCAAGATCTATTGCAAGACTTTTTCAAGGGGAAGGATTTATGCAAGAGTATCAACCCGGACGAAGCTGTTGCTTATGGTGCAGCTGTTCAAGCTGCTATTTTGAGCAAAGGCTTTAAGAATGTCCCGAACTTGGTGTTGCGAGATGTTACCCCATTGTCGCTTGGTATTTCAGCTGGTGCTGGTTGTGTCATGAGTGTGGTGGTTCCTAGGAACACTTCCATACCTGTGACAAAGACAGAACGATTTCACACCGCTAAAGATAACCATTGTGAAGTCTCGATTAATGTTTATGAGGGAGAGAGAGCAAAAGCTACTGACAATAATCTGCTTGGTTTTTTCACCCTTTCTTGTCTTCCTGGTGCTCCTCGGGGCCAACCCTTAGATGTATGCTTTGGTGTCGATGAAAATGGTATCCTAACTGTTTCTGCTAAGGAAATATCTACAGGAAATACGAACAAGATCACCATAACCAATGAAAAAGAAAGATTGTCAACCTTGGAAATTAAAAAAATGATTGAAGAAGCTGAGAGATACCACATGGAAGATAAGAAATTTTTACAGAAGGCCAAGGTAATGAATGCATTGGATTATTGTGTTTACGATGTGAAGAATGCTTTAAAGAAAGATGTGAATTTAAAGCTCTCCTCTCAAGAAATTGAGAAGATCAACAATGCAATTATTGTGGCCACAAGTTTGCTTGATAAGAATAACAAACAAAATGAAATAGATGTTCTTGAGTCTCATCTGAAGGAGATGGAGAGCATGTTGAAGCACGTTATGGCCAAGACTGGCTAG
Protein sequence

>Msa0321670

MAKKHEGRAVGIDLGTTNSCVGVWMEHQNRVEIIHNDQGNRTTPSFVAFTHDQRLIGDAAKNQVASNPQNTIFDAKRLIGRKFSDPVVQNDVMLWPFKVIAGMNDKPMITLKYKGQESQFCAEEISSMILTKMREVAEAFMESPVKNAVVTVPAYFNDSQRKATIDAGAIAGLNVIRIINEPTAAAIAYGLDKRSDCDGKRNIFVFDLGGGTFDVSILTIKGDVFEVKATAGNTHLGGEDFDNRTVNYFVEEFRKKNKVDISGNPRALRRLRTACERAKRTLSFAFVTNVEVDSLFQGIDFSSSITRAKFEEINMDLFNECMTTVENCLSDSKMQIADIDDVVLVGGSSRIPKVQDLLQDFFKGKDLCKSINPDEAVAYGAAVQAAILSKGFKNVPNLVLRDVTPLSLGISAGAGCVMSVVVPRNTSIPVTKTERFHTAKDNHCEVSINVYEGERAKATDNNLLGFFTLSCLPGAPRGQPLDVCFGVDENGILTVSAKEISTGNTNKITITNEKERLSTLEIKKMIEEAERYHMEDKKFLQKAKVMNALDYCVYDVKNALKKDVNLKLSSQEIEKINNAIIVATSLLDKNNKQNEIDVLESHLKEMESMLKHVMAKTG*