Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0202340 | AES65449.2 | 61.538 | 78 | 19 | 1 | 9 | 86 | 199 | 265 | 4.69e-17 | 83.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0202340 | G7IIK6 | 61.538 | 78 | 19 | 1 | 9 | 86 | 199 | 265 | 2.24e-17 | 83.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0202340 | Msa0202370 | 0.808764 | 2.637699e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0202340 | MtrunA17_Chr2g0299461 | 61.538 | 78 | 19 | 1 | 9 | 86 | 251 | 317 | 8.36e-21 | 83.6 |
| Msa0202340 | MtrunA17_Chr2g0299421 | 48.718 | 78 | 29 | 1 | 9 | 86 | 207 | 273 | 1.96e-16 | 71.2 |
| Msa0202340 | MtrunA17_Chr2g0299401 | 46.154 | 78 | 31 | 1 | 9 | 86 | 203 | 269 | 3.54e-14 | 65.1 |
| Msa0202340 | MtrunA17_Chr2g0299391 | 45.333 | 75 | 30 | 1 | 7 | 81 | 204 | 267 | 1.75e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTAAGGTTACATCATATA+TGG | 0.311999 | 2_1:-24699156 | Msa0202340:CDS |
| ATGCAAAATTACATCATATA+TGG | 0.340076 | 2_1:-24699242 | Msa0202340:CDS |
| AACTCGTGCGCACTTTGAAC+GGG | 0.413947 | 2_1:+24699179 | None:intergenic |
| GGCAGGGTCCTTACAGTTCA+CGG | 0.470147 | 2_1:-24699135 | Msa0202340:CDS |
| GGCAGGGTCCTTACAGTTCA+CGG | 0.470147 | 2_1:-24699221 | Msa0202340:CDS |
| CAATAGCGGTATTGTTAGCT+AGG | 0.475110 | 2_1:-24698960 | None:intergenic |
| AAACTCGTGCGCACTTTGAA+CGG | 0.492795 | 2_1:+24699178 | None:intergenic |
| AAAATTACATCATATATGGC+AGG | 0.503422 | 2_1:-24699238 | Msa0202340:CDS |
| AAGGTTACATCATATATGGC+AGG | 0.506738 | 2_1:-24699152 | Msa0202340:CDS |
| CAGAAGAACCGTGAACTGTA+AGG | 0.532860 | 2_1:+24699213 | None:intergenic |
| AGTTGTTATGAGCTTCATCA+AGG | 0.533763 | 2_1:-24698996 | Msa0202340:CDS |
| AAGTGCGCACGAGTTTGCTA+AGG | 0.546002 | 2_1:-24699171 | Msa0202340:CDS |
| TAGAAGAACCGTGAACTGTA+AGG | 0.552489 | 2_1:+24699127 | None:intergenic |
| AAGTGCGCACGAGTTTGCTA+AGG | 0.575019 | 2_1:-24699085 | Msa0202340:intron |
| AGCTAACAATACCGCTATTG+TGG | 0.576694 | 2_1:+24698963 | None:intergenic |
| ACTCGTGCGCACTTTGAACA+AGG | 0.595182 | 2_1:+24699094 | None:intergenic |
| AAATTACATCATATATGGCA+GGG | 0.602506 | 2_1:-24699237 | Msa0202340:CDS |
| AGGTTACATCATATATGGCA+GGG | 0.638088 | 2_1:-24699151 | Msa0202340:CDS |
| ACTCGTGCGCACTTTGAACG+GGG | 0.650817 | 2_1:+24699180 | None:intergenic |
| GATGAAGCTCATAACAACTG+AGG | 0.686040 | 2_1:+24699000 | None:intergenic |
| GAAAGCACTGACCACAATAG+CGG | 0.699989 | 2_1:-24698974 | Msa0202340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCAAAATTACATCATATA+TGG | - | chr2_1:24698961-24698980 | Msa0202340:CDS | 20.0% |
| ! | AAAATTACATCATATATGGC+AGG | - | chr2_1:24698965-24698984 | Msa0202340:CDS | 25.0% |
| ! | AAATTACATCATATATGGCA+GGG | - | chr2_1:24698966-24698985 | Msa0202340:CDS | 25.0% |
| TGCTAAGGTTACATCATATA+TGG | - | chr2_1:24699047-24699066 | Msa0202340:intron | 30.0% | |
| ATGATATGCAACTTGTGATT+TGG | + | chr2_1:24699149-24699168 | None:intergenic | 30.0% | |
| AAGGTTACATCATATATGGC+AGG | - | chr2_1:24699051-24699070 | Msa0202340:intron | 35.0% | |
| AGGTTACATCATATATGGCA+GGG | - | chr2_1:24699052-24699071 | Msa0202340:intron | 35.0% | |
| AGTTGTTATGAGCTTCATCA+AGG | - | chr2_1:24699207-24699226 | Msa0202340:CDS | 35.0% | |
| TAGAAGAACCGTGAACTGTA+AGG | + | chr2_1:24699079-24699098 | None:intergenic | 40.0% | |
| GATGAAGCTCATAACAACTG+AGG | + | chr2_1:24699206-24699225 | None:intergenic | 40.0% | |
| AGCTAACAATACCGCTATTG+TGG | + | chr2_1:24699243-24699262 | None:intergenic | 40.0% | |
| CAGAAGAACCGTGAACTGTA+AGG | + | chr2_1:24698993-24699012 | None:intergenic | 45.0% | |
| AAACTCGTGCGCACTTTGAA+CGG | + | chr2_1:24699028-24699047 | None:intergenic | 45.0% | |
| !! | GAAAGCACTGACCACAATAG+CGG | - | chr2_1:24699229-24699248 | Msa0202340:CDS | 45.0% |
| AACTCGTGCGCACTTTGAAC+GGG | + | chr2_1:24699027-24699046 | None:intergenic | 50.0% | |
| !! | AAGTGCGCACGAGTTTGCTA+AGG | - | chr2_1:24699032-24699051 | Msa0202340:intron | 50.0% |
| ACTCGTGCGCACTTTGAACA+AGG | + | chr2_1:24699112-24699131 | None:intergenic | 50.0% | |
| !! | AAGTGCGCACGAGTTTGCTA+AGG | - | chr2_1:24699032-24699051 | Msa0202340:intron | 50.0% |
| GGCAGGGTCCTTACAGTTCA+CGG | - | chr2_1:24698982-24699001 | Msa0202340:CDS | 55.0% | |
| ACTCGTGCGCACTTTGAACG+GGG | + | chr2_1:24699026-24699045 | None:intergenic | 55.0% | |
| GGCAGGGTCCTTACAGTTCA+CGG | - | chr2_1:24698982-24699001 | Msa0202340:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 24698961 | 24699264 | 24698961 | ID=Msa0202340;Name=Msa0202340 |
| chr2_1 | mRNA | 24698961 | 24699264 | 24698961 | ID=Msa0202340-mRNA-1;Parent=Msa0202340;Name=Msa0202340-mRNA-1;_AED=0.42;_eAED=0.60;_QI=0|0|0|1|0|0|2|0|86 |
| chr2_1 | exon | 24699086 | 24699264 | 24699086 | ID=Msa0202340-mRNA-1:exon:9474;Parent=Msa0202340-mRNA-1 |
| chr2_1 | exon | 24698961 | 24699042 | 24698961 | ID=Msa0202340-mRNA-1:exon:9473;Parent=Msa0202340-mRNA-1 |
| chr2_1 | CDS | 24699086 | 24699264 | 24699086 | ID=Msa0202340-mRNA-1:cds;Parent=Msa0202340-mRNA-1 |
| chr2_1 | CDS | 24698961 | 24699042 | 24698961 | ID=Msa0202340-mRNA-1:cds;Parent=Msa0202340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
