AlfalfaGEDB | Msa0203550

M. sativa cultivar zhongmu-4 / Msa0203550

KEGG: K14486
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009791 GO:0009889 GO:0010015 GO:0010033 GO:0010101 GO:0010102 GO:0010311 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0046983 GO:0048364 GO:0048366 GO:0048367 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:1905392 GO:1905393 GO:2000112 GO:2001141
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203550	XP_013463581.1	84.538	692	80	8	37	707	1	686	0.0	1144

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203550	sp\|Q8RYC8\|ARFS_ARATH	80.658	486	84	4	37	518	1	480	0.0	801

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203550	A0A072V7K3	84.538	692	80	8	37	707	1	686	0.0	1144

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TFs/TRs:

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Gene ID	Type	Classification
Msa0203550	TF	B3-ARF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0203550	Msa0655660	0.809439	1.891113e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203550	MtrunA17_Chr2g0301251	84.538	692	80	8	37	707	1	686	0.0	1144
Msa0203550	MtrunA17_Chr4g0070181	85.542	498	55	6	37	523	1	492	0.0	866
Msa0203550	MtrunA17_Chr8g0389081	61.996	571	168	14	51	588	21	575	0.0	586
Msa0203550	MtrunA17_Chr2g0285461	60.707	481	164	12	50	521	14	478	0.0	541
Msa0203550	MtrunA17_Chr8g0376771	57.996	519	187	14	37	543	1	500	2.10e-178	534
Msa0203550	MtrunA17_Chr5g0433301	58.750	480	169	12	37	500	1	467	7.62e-176	525
Msa0203550	MtrunA17_Chr1g0156321	68.097	373	111	5	51	419	35	403	3.00e-174	522
Msa0203550	MtrunA17_Chr3g0109391	52.827	566	206	15	53	586	20	556	4.10e-173	519
Msa0203550	MtrunA17_Chr8g0385791	50.000	388	192	2	30	416	30	416	6.21e-128	401
Msa0203550	MtrunA17_Chr2g0276781	53.982	339	151	4	59	395	23	358	4.50e-120	375

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203550	AT1G19220.1	80.658	486	84	4	37	518	1	480	0.0	801
Msa0203550	AT5G20730.2	78.351	485	97	5	37	514	1	484	0.0	770
Msa0203550	AT5G20730.3	78.189	486	97	6	37	514	1	485	0.0	765
Msa0203550	AT5G20730.1	78.189	486	97	6	37	514	1	485	0.0	765
Msa0203550	AT1G30330.1	68.090	398	121	6	37	430	1	396	0.0	543
Msa0203550	AT1G30330.3	69.531	384	111	6	51	430	17	398	1.90e-179	538
Msa0203550	AT1G30330.2	69.531	384	111	6	51	430	17	398	1.90e-179	538
Msa0203550	AT5G37020.2	59.821	448	154	11	37	466	1	440	1.39e-171	513
Msa0203550	AT5G37020.1	59.821	448	154	11	37	466	1	440	8.33e-171	512
Msa0203550	AT1G19850.2	57.456	456	169	9	51	491	34	479	5.72e-169	509

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Find 164 sgRNAs with CRISPR-Local

Find 362 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATTCTGAGGGTTGT+TGG	0.430251	2_1:+27480812	None:intergenic
AACAAGCCGGTCTCTTACAG+CGG	0.618906	2_1:-27478309	Msa0203550:CDS
AACTAAAACAAGTGAAACTA+AGG	0.430778	2_1:+27482980	None:intergenic
AAGAGAGCTATGCCATGGCT+TGG	0.516884	2_1:-27479027	Msa0203550:CDS
AAGAGGCATCTATTGACTAC+TGG	0.515402	2_1:-27480436	Msa0203550:CDS
AAGATTTGCATGACACTACA+TGG	0.497978	2_1:-27480577	Msa0203550:CDS
AATCACTGGGATCAGTGACT+TGG	0.541031	2_1:-27479877	Msa0203550:CDS
AATGGAATCACAAATTCACA+GGG	0.688736	2_1:+27480002	None:intergenic
AATGGTTACTTGGCTAATTC+TGG	0.236946	2_1:-27482622	Msa0203550:CDS
ACAACAAGGCTTCCAACAGG+CGG	0.694325	2_1:+27481908	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAGCTCATATTTCAATGT+CGG	-	chr2_1:27477961-27477980	Msa0203550:CDS	30.0%
AAAAAGCAGAAGCAGAAAAT+GGG	+	chr2_1:27478128-27478147	None:intergenic	30.0%
AAAAAAGCAGAAGCAGAAAA+TGG	+	chr2_1:27478129-27478148	None:intergenic	30.0%
GAATATTGAAGAAGATTCAG+AGG	-	chr2_1:27478369-27478388	Msa0203550:CDS	30.0%
CTTTGTGGTAAGAATCAAAA+AGG	-	chr2_1:27478463-27478482	Msa0203550:CDS	30.0%
TTGATATTGTGGAATACTTG+AGG	-	chr2_1:27478550-27478569	Msa0203550:CDS	30.0%
TGTTCAGAGATTAATGAAAC+AGG	+	chr2_1:27478756-27478775	None:intergenic	30.0%
AACCCCTTACTCATATTTAT+TGG	+	chr2_1:27478860-27478879	None:intergenic	30.0%
GTGAGAAGGTGATTAAATTA+AGG	+	chr2_1:27479084-27479103	None:intergenic	30.0%
TTGATTTCTGTACCTATAAC+AGG	-	chr2_1:27479300-27479319	Msa0203550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	27477915	27483399	27477915	ID=Msa0203550;Name=Msa0203550
chr2_1	mRNA	27477915	27483399	27477915	ID=Msa0203550-mRNA-1;Parent=Msa0203550;Name=Msa0203550-mRNA-1;_AED=0.17;_eAED=0.23;_QI=0\|0.58\|0.5\|1\|0.58\|0.61\|18\|0\|757
chr2_1	exon	27483396	27483399	27483396	ID=Msa0203550-mRNA-1:exon:10181;Parent=Msa0203550-mRNA-1
chr2_1	exon	27482924	27483021	27482924	ID=Msa0203550-mRNA-1:exon:10180;Parent=Msa0203550-mRNA-1
chr2_1	exon	27482617	27482665	27482617	ID=Msa0203550-mRNA-1:exon:10179;Parent=Msa0203550-mRNA-1
chr2_1	exon	27481880	27481992	27481880	ID=Msa0203550-mRNA-1:exon:10178;Parent=Msa0203550-mRNA-1
chr2_1	exon	27481708	27481803	27481708	ID=Msa0203550-mRNA-1:exon:10177;Parent=Msa0203550-mRNA-1
chr2_1	exon	27481001	27481057	27481001	ID=Msa0203550-mRNA-1:exon:10176;Parent=Msa0203550-mRNA-1
chr2_1	exon	27480726	27480881	27480726	ID=Msa0203550-mRNA-1:exon:10175;Parent=Msa0203550-mRNA-1
chr2_1	exon	27480555	27480639	27480555	ID=Msa0203550-mRNA-1:exon:10174;Parent=Msa0203550-mRNA-1
chr2_1	exon	27480376	27480466	27480376	ID=Msa0203550-mRNA-1:exon:10173;Parent=Msa0203550-mRNA-1
chr2_1	exon	27480127	27480291	27480127	ID=Msa0203550-mRNA-1:exon:10172;Parent=Msa0203550-mRNA-1
chr2_1	exon	27479836	27480031	27479836	ID=Msa0203550-mRNA-1:exon:10171;Parent=Msa0203550-mRNA-1
chr2_1	exon	27479284	27479422	27479284	ID=Msa0203550-mRNA-1:exon:10170;Parent=Msa0203550-mRNA-1
chr2_1	exon	27478983	27479078	27478983	ID=Msa0203550-mRNA-1:exon:10169;Parent=Msa0203550-mRNA-1
chr2_1	exon	27478762	27478970	27478762	ID=Msa0203550-mRNA-1:exon:10168;Parent=Msa0203550-mRNA-1
chr2_1	exon	27478509	27478743	27478509	ID=Msa0203550-mRNA-1:exon:10167;Parent=Msa0203550-mRNA-1
chr2_1	exon	27478277	27478497	27478277	ID=Msa0203550-mRNA-1:exon:10166;Parent=Msa0203550-mRNA-1
chr2_1	exon	27478163	27478269	27478163	ID=Msa0203550-mRNA-1:exon:10165;Parent=Msa0203550-mRNA-1
chr2_1	exon	27477915	27478071	27477915	ID=Msa0203550-mRNA-1:exon:10164;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27483396	27483399	27483396	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27482924	27483021	27482924	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27482617	27482665	27482617	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27481880	27481992	27481880	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27481708	27481803	27481708	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27481001	27481057	27481001	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27480726	27480881	27480726	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27480555	27480639	27480555	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27480376	27480466	27480376	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27480127	27480291	27480127	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27479836	27480031	27479836	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27479284	27479422	27479284	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27478983	27479078	27478983	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27478762	27478970	27478762	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27478509	27478743	27478509	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27478277	27478497	27478277	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27478163	27478269	27478163	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1
chr2_1	CDS	27477915	27478071	27477915	ID=Msa0203550-mRNA-1:cds;Parent=Msa0203550-mRNA-1

Gene Sequence

>Msa0203550

ATGAATTTTAGCTTGTTGGGCACCCTTAGTTTCACTTGTTTTAGTTGCAAAGTTGTGGTTTTTGGCTTTTTTGAAGTTTTGAATATTGAAGAAGATTCAGAGGGTATAATGAAGGCTCCTTCTAATGGTTACTTGGCTAATTCTGGTGAAGGTGAAAGGAAGACCATCAATTCAGAGTTATGGCATGCTTGTGCCGGGCCATTGGTTTCTATACCGCCTGTTGGAAGCCTTGTTGTTTACTTTCCTCAAGGACATAGTGAACAAGTTGCAGCATCTATGCAAAAGGAGGCTGACATCATACCAAGTTACCCTAACCTTCCATCCAAGTTGATCTGTATGCTTCACAATGTTGCCCTGCATGCTGACCCTGAAACCGACGAGGTCTATGCACAGATGACCCTTCAACCTGTAAATAAATATGACAAGGAAGCAATGCTAGCTTCAGACATGGGTCTCAAACAAAACCAACAACCCTCAGAATTCTTTTGCAAAACTCTAACAGCTAGTGACACTAGCACTCACGGTGGATTTTCAGTGCCTCGTAGAGCTGCTGAGAAAATATTCCCACCTCTGGACTTCTCAATGCAACCGCCAGCTCAGGAAATAGTTGCCAAAGATTTGCATGACACTACATGGACATTTAGACATATTTATCGCGGACAACCAAAGAGGCATCTATTGACTACTGGTTGGAGTGTCTTCGTTAGCACAAAAAGGCTCTTTGCTGGAGATTCTGTTCTTTTTATCAGAGATGAAAAGCAGCAACTTCTCTTAGGTATAAAGAGATCCAGTAGACAGCAACCAGCACTATCTTCATCGGTAATATCAAGTGACAGCATGCATATCGGCATTCTTGCTGCTGCGGCTCATGCTGCTTCGAATAACAGTCCATTTACTATATTTTATAATCCAAGGACTAGCCCCTGTGAATTTGTGATTCCATTGGCAAAGTATAACAAAGCTCTGTACACTCATGTTTCCCTTGGAATGAGATTCAGAATGATGTTTGAGACAGAGGATTCCGGAGTACGCAGATATATGGGCACAATCACTGGGATCAGTGACTTGGATCCTGTCCGCTGGAAAAATTCACAATGGCGCAATCTTCAGGTTGGATGGGACGAATCAACAGCTGGTGAGCGCCCAAGTAGAGTTTCAATTTGGGACATTGAACCAGTTGTGACTCCTTTTTACATTTGTCCACCTCCGTTTTTTAGGCCGAAGTTCCCGAAGCAACCAGGAATGCCAGAAGATGAGTCTGAAATCGAAAATGCATTCAAGAGAGCTATGCCATGGCTTGGAGATGAACTCGGCATGAAGGATACCTCAAGTTCAATCTTCCCTGTACAATGGATGAGTATGCAGCAGAATAATAATAATAATAATCAGTTTTCCGCTGCTCAATCAGGGCTTTTACCTCCATCCATGCTTTCGTCAAATGCAATGCATGGTAACCTTAGCACTGATGACCCGTCCAAGATACTGAACTTCCAATCCCCTGCTTCGCTCTCTGCTCCAAGTCTTCAATTTAATAAACCTAATTTACCGAACCAACCCCCGGCCTCGTGGTCTCAGCAACAGCAGCAGAAGATGCAGTCGTTGTTGCCGTTAAACCAGCTGCAGCAGCAGCAGCAACAGCAGCAGAGGCAACAGCAGCTGGCTGGACCACAAAATCTGCCGCAGCCACAGCAGCAACAGCCTCAACTGACTCAACAGAATCCGCAGCAGCCTCAACAGCAGCAACAACACCAACAGCCTCAAAATACGACTATGAATAACGGCACAATTGATTCCGAATCAGTGTGTACAGCAACCAGTAAACTACTCTCAGCTTCAGCAACAGTTACTCTCAGGAAGTATCCAATCCCAACAAAATTTCCAATCAGCTGGCAAGAATGGATTGATAATGACATCCTTACCACAAGACTCGCAATTCCAGCAACAAATAGACCAACAACAAGCCGGTCTCTTACAGCGGCAGCAGCAACAGAATCAGTTGCAGCAATCTTCTTATTGCAACAAAGCATGTTGCAAAGGGCACCACAACAATCACAAATGTCTCAAATTTCGCCGCAAAACATCTCAGACCAACAGCAACAGTTGCAGTTGTTACAGAATCTCAGCTTCTGCCGCAACAAAGCCAGCAATTACCTCAGCAGCCTATGTCCCAGCATCAGCCCCAACAGCTCGGAAACAACGCATTCTCAATGGAGAAGCTTCTCAACAACAATTACTCTTCTTCACCTCTTATGCATCCACAGCAGCTCCCTGTGA

Protein sequence

>Msa0203550

MNFSLLGTLSFTCFSCKVVVFGFFEVLNIEEDSEGIMKAPSNGYLANSGEGERKTINSELWHACAGPLVSIPPVGSLVVYFPQGHSEQVAASMQKEADIIPSYPNLPSKLICMLHNVALHADPETDEVYAQMTLQPVNKYDKEAMLASDMGLKQNQQPSEFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFSMQPPAQEIVAKDLHDTTWTFRHIYRGQPKRHLLTTGWSVFVSTKRLFAGDSVLFIRDEKQQLLLGIKRSSRQQPALSSSVISSDSMHIGILAAAAHAASNNSPFTIFYNPRTSPCEFVIPLAKYNKALYTHVSLGMRFRMMFETEDSGVRRYMGTITGISDLDPVRWKNSQWRNLQVGWDESTAGERPSRVSIWDIEPVVTPFYICPPPFFRPKFPKQPGMPEDESEIENAFKRAMPWLGDELGMKDTSSSIFPVQWMSMQQNNNNNNQFSAAQSGLLPPSMLSSNAMHGNLSTDDPSKILNFQSPASLSAPSLQFNKPNLPNQPPASWSQQQQQKMQSLLPLNQLQQQQQQQQRQQQLAGPQNLPQPQQQQPQLTQQNPQQPQQQQQHQQPQNTTMNNGTIDSESVCTATSKLLSASATVTLRKYPIPTKFPISWQEWIDNDILTTRLAIPATNRPTTSRSLTAAAATESVAAIFLLQQSMLQRAPQQSQMSQISPQNISDQQQQLQLLQNLSFCRNKASNYLSSLCPSISPNSSETTHSQWRSFSTTITLLHLLCIHSSSL*