Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0203910 | Msa1393500 | 0.803706 | 3.066618e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0203910 | MtrunA17_Chr5g0398751 | 72.500 | 40 | 11 | 0 | 40 | 79 | 77 | 116 | 2.78e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGTTTGATGAATGTATT+AGG | 0.235303 | 2_1:+28497012 | Msa0203910:CDS |
| CGATGGATTTGAGTTGGGCT+TGG | 0.312614 | 2_1:-28496866 | None:intergenic |
| ATTTGAGTTGGGCTTGGGTC+GGG | 0.416027 | 2_1:-28496860 | None:intergenic |
| CGGCGACGATGGATTTGAGT+TGG | 0.421727 | 2_1:-28496872 | None:intergenic |
| GATGGATTTGAGTTGGGCTT+GGG | 0.428812 | 2_1:-28496865 | None:intergenic |
| GATTTGAGTTGGGCTTGGGT+CGG | 0.446248 | 2_1:-28496861 | None:intergenic |
| GGCGACGATGGATTTGAGTT+GGG | 0.457758 | 2_1:-28496871 | None:intergenic |
| GTTCTCGATAAAGATGCTAC+TGG | 0.465422 | 2_1:+28496922 | Msa0203910:CDS |
| GAAATCGAAAGGAGAACATT+CGG | 0.467430 | 2_1:-28496892 | None:intergenic |
| TACCGCCGAGAGAAAGCAAC+AGG | 0.474309 | 2_1:-28496836 | None:intergenic |
| GGAATCCTGTTGCTTTCTCT+CGG | 0.475828 | 2_1:+28496831 | Msa0203910:CDS |
| AGGAGAACATTCGGCGACGA+TGG | 0.475961 | 2_1:-28496883 | None:intergenic |
| TGAGTTTGATGAATGTATTA+GGG | 0.475992 | 2_1:+28497013 | Msa0203910:CDS |
| GAAGTTGATGTTGGGTCTGA+TGG | 0.478332 | 2_1:+28497035 | Msa0203910:CDS |
| TGTATTAGGGAAGTTGATGT+TGG | 0.500218 | 2_1:+28497026 | Msa0203910:CDS |
| AAGATCGCACCATCGAAGCT+TGG | 0.500822 | 2_1:+28496810 | Msa0203910:CDS |
| TGCGATCTTTCCGTGGTTGT+CGG | 0.518371 | 2_1:-28496796 | None:intergenic |
| AAACAATTGCATTCATCACT+TGG | 0.521261 | 2_1:-28497098 | None:intergenic |
| ATACATTCATCAAACTCAGC+AGG | 0.529884 | 2_1:-28497008 | None:intergenic |
| GTATTAGGGAAGTTGATGTT+GGG | 0.530223 | 2_1:+28497027 | Msa0203910:CDS |
| ATGAAGATTCATCTCAAGAA+TGG | 0.533917 | 2_1:+28497069 | Msa0203910:CDS |
| CTTGAGATGAATCTTCATAA+CGG | 0.540411 | 2_1:-28497064 | None:intergenic |
| AAGTTGATGTTGGGTCTGAT+GGG | 0.550763 | 2_1:+28497036 | Msa0203910:CDS |
| GAACTTTGAGAGAAATCGAA+AGG | 0.580421 | 2_1:-28496903 | None:intergenic |
| AACAGGATTCCAAGCTTCGA+TGG | 0.609426 | 2_1:-28496819 | None:intergenic |
| ATCCTGTTGCTTTCTCTCGG+CGG | 0.610054 | 2_1:+28496834 | Msa0203910:CDS |
| CTCTTCGACACCGACAACCA+CGG | 0.610443 | 2_1:+28496786 | Msa0203910:CDS |
| TCGATGGTGCGATCTTTCCG+TGG | 0.631360 | 2_1:-28496803 | None:intergenic |
| AAGATTCATCTCAAGAATGG+TGG | 0.655263 | 2_1:+28497072 | Msa0203910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAGTTTGATGAATGTATTA+GGG | + | chr2_1:28497013-28497032 | Msa0203910:CDS | 25.0% |
| !!! | CATCATATTTTGACGAGTAT+TGG | + | chr2_1:28496967-28496986 | Msa0203910:CDS | 30.0% |
| CTGAGTTTGATGAATGTATT+AGG | + | chr2_1:28497012-28497031 | Msa0203910:CDS | 30.0% | |
| CTTGAGATGAATCTTCATAA+CGG | - | chr2_1:28497067-28497086 | None:intergenic | 30.0% | |
| ATGAAGATTCATCTCAAGAA+TGG | + | chr2_1:28497069-28497088 | Msa0203910:CDS | 30.0% | |
| AAACAATTGCATTCATCACT+TGG | - | chr2_1:28497101-28497120 | None:intergenic | 30.0% | |
| GAAATCGAAAGGAGAACATT+CGG | - | chr2_1:28496895-28496914 | None:intergenic | 35.0% | |
| ! | GAACTTTGAGAGAAATCGAA+AGG | - | chr2_1:28496906-28496925 | None:intergenic | 35.0% |
| ATACATTCATCAAACTCAGC+AGG | - | chr2_1:28497011-28497030 | None:intergenic | 35.0% | |
| !! | TGTATTAGGGAAGTTGATGT+TGG | + | chr2_1:28497026-28497045 | Msa0203910:CDS | 35.0% |
| !! | GTATTAGGGAAGTTGATGTT+GGG | + | chr2_1:28497027-28497046 | Msa0203910:CDS | 35.0% |
| AAGATTCATCTCAAGAATGG+TGG | + | chr2_1:28497072-28497091 | Msa0203910:CDS | 35.0% | |
| GTTCTCGATAAAGATGCTAC+TGG | + | chr2_1:28496922-28496941 | Msa0203910:CDS | 40.0% | |
| !! | AAGTTGATGTTGGGTCTGAT+GGG | + | chr2_1:28497036-28497055 | Msa0203910:CDS | 40.0% |
| AACAGGATTCCAAGCTTCGA+TGG | - | chr2_1:28496822-28496841 | None:intergenic | 45.0% | |
| !! | GGAATCCTGTTGCTTTCTCT+CGG | + | chr2_1:28496831-28496850 | Msa0203910:CDS | 45.0% |
| !! | GATGGATTTGAGTTGGGCTT+GGG | - | chr2_1:28496868-28496887 | None:intergenic | 45.0% |
| !! | GAAGTTGATGTTGGGTCTGA+TGG | + | chr2_1:28497035-28497054 | Msa0203910:CDS | 45.0% |
| TGCGATCTTTCCGTGGTTGT+CGG | - | chr2_1:28496799-28496818 | None:intergenic | 50.0% | |
| AAGATCGCACCATCGAAGCT+TGG | + | chr2_1:28496810-28496829 | Msa0203910:CDS | 50.0% | |
| !! | ATCCTGTTGCTTTCTCTCGG+CGG | + | chr2_1:28496834-28496853 | Msa0203910:CDS | 50.0% |
| !! | ATTTGAGTTGGGCTTGGGTC+GGG | - | chr2_1:28496863-28496882 | None:intergenic | 50.0% |
| !! | GATTTGAGTTGGGCTTGGGT+CGG | - | chr2_1:28496864-28496883 | None:intergenic | 50.0% |
| !! | CGATGGATTTGAGTTGGGCT+TGG | - | chr2_1:28496869-28496888 | None:intergenic | 50.0% |
| ! | GGCGACGATGGATTTGAGTT+GGG | - | chr2_1:28496874-28496893 | None:intergenic | 50.0% |
| CTCTTCGACACCGACAACCA+CGG | + | chr2_1:28496786-28496805 | Msa0203910:CDS | 55.0% | |
| TCGATGGTGCGATCTTTCCG+TGG | - | chr2_1:28496806-28496825 | None:intergenic | 55.0% | |
| TACCGCCGAGAGAAAGCAAC+AGG | - | chr2_1:28496839-28496858 | None:intergenic | 55.0% | |
| ! | CGGCGACGATGGATTTGAGT+TGG | - | chr2_1:28496875-28496894 | None:intergenic | 55.0% |
| AGGAGAACATTCGGCGACGA+TGG | - | chr2_1:28496886-28496905 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 28496766 | 28497122 | 28496766 | ID=Msa0203910;Name=Msa0203910 |
| chr2_1 | mRNA | 28496766 | 28497122 | 28496766 | ID=Msa0203910-mRNA-1;Parent=Msa0203910;Name=Msa0203910-mRNA-1;_AED=0.54;_eAED=0.82;_QI=0|-1|0|1|-1|1|1|0|118 |
| chr2_1 | exon | 28496766 | 28497122 | 28496766 | ID=Msa0203910-mRNA-1:exon:10338;Parent=Msa0203910-mRNA-1 |
| chr2_1 | CDS | 28496766 | 28497122 | 28496766 | ID=Msa0203910-mRNA-1:cds;Parent=Msa0203910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
