AlfalfaGEDB | Msa0204230

M. sativa cultivar zhongmu-4 / Msa0204230

KEGG: K04681
GO: GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0204230	PNY08177.1	71.845	103	13	1	1	87	48	150	3.99e-38	146

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0204230	sp\|Q9SLZ4\|RBR1_PEA	70.874	103	14	1	1	87	48	150	3.03e-40	144

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0204230	A0A2K3NYS1	71.845	103	13	1	1	87	48	150	1.91e-38	146

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0204230	Msa1081670	0.810770	9.768873e-51	-8.615850e-47
Msa0204230	Msa1082240	0.815382	9.515668e-52	-8.615850e-47
Msa0204230	Msa1319490	0.913361	6.726025e-84	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0204230	MtrunA17_Chr8g0353751	78.641	103	6	1	1	87	46	148	2.11e-38	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0204230	AT3G12280.2	46.602	103	39	2	1	87	42	144	6.44e-23	92.0
Msa0204230	AT3G12280.1	46.602	103	39	2	1	87	42	144	6.44e-23	92.0

Find 46 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAAATCGATTGCTTAAAAT+TGG	0.181934	2_1:-29141722	None:intergenic
AGATGGAAGTTGGGGATATT+AGG	0.184788	2_1:-29140542	None:intergenic
GCTTCTGCTGTTTGGCCTTT+TGG	0.186951	2_1:+29141118	Msa0204230:five_prime_UTR
CGAGAGAGTGAGATGGAAGT+TGG	0.292056	2_1:-29140552	None:intergenic
TTCCCTTCTCCGTCATTTCA+CGG	0.319534	2_1:+29140638	Msa0204230:five_prime_UTR
GATGGAAGTTGGGGATATTA+GGG	0.321992	2_1:-29140541	None:intergenic
AGAGAGAAAGTTGAATGAAT+TGG	0.333762	2_1:-29140489	None:intergenic
TGAACAGAGTAACGTCAATA+AGG	0.358090	2_1:+29141926	Msa0204230:intron
CATGGCAAATGTTTCATCAA+TGG	0.366338	2_1:+29141331	Msa0204230:CDS
GAGAGAGTGAGATGGAAGTT+GGG	0.366637	2_1:-29140551	None:intergenic
ATTTGCCATGAGAAGATGTT+TGG	0.379727	2_1:-29141318	None:intergenic
AAAGATGAGATTGAGAATAC+TGG	0.403583	2_1:+29141530	Msa0204230:CDS
CAGAAGAAGCAGAGCGGTGC+TGG	0.404107	2_1:+29141450	Msa0204230:CDS
AATGTTTCATCAATGGGAAA+TGG	0.408483	2_1:+29141338	Msa0204230:CDS
TGGGGATATTAGGGTTTCAA+TGG	0.409813	2_1:-29140532	None:intergenic
GCAATCGATTTAGCTCTGAT+TGG	0.415284	2_1:+29141732	Msa0204230:CDS
AAAGAAGCAATGGATCTGTT+TGG	0.423829	2_1:+29141290	Msa0204230:exon
ATGGCAAATGTTTCATCAAT+GGG	0.427540	2_1:+29141332	Msa0204230:CDS
ATAGACTGCATTTGTGTTTG+TGG	0.436210	2_1:+29141013	Msa0204230:five_prime_UTR
TTGTGTTTGTGGACCGTGGC+AGG	0.441265	2_1:+29141024	Msa0204230:five_prime_UTR
CAATCGATTTAGCTCTGATT+GGG	0.455232	2_1:+29141733	Msa0204230:CDS
CTGCTGTTATAGCTCTCGAC+TGG	0.487878	2_1:+29141087	Msa0204230:five_prime_UTR
ACTTCCTCAGTTTGTTGTCA+AGG	0.489779	2_1:+29141694	Msa0204230:CDS
ACGAATAAACCGTGAAATGA+CGG	0.502367	2_1:-29140647	None:intergenic
GTTGCTCAGGACGTAATTTG+AGG	0.514531	2_1:+29140988	Msa0204230:intron
GGGGATATTAGGGTTTCAAT+GGG	0.524270	2_1:-29140531	None:intergenic
CGATGTGGTCAGAGATGAGA+AGG	0.536025	2_1:-29140615	None:intergenic
AGAAAACTGTAAAGAAGCAA+TGG	0.539140	2_1:+29141280	Msa0204230:exon
AGAGAGTGAGATGGAAGTTG+GGG	0.540686	2_1:-29140550	None:intergenic
CACAAAACTGAAACACCAAA+AGG	0.551426	2_1:-29141133	None:intergenic
GCAGCTGAATGAGCTAGCAT+TGG	0.552255	2_1:+29141254	Msa0204230:intron
AGTAGATCGAGAGAGTGAGA+TGG	0.559499	2_1:-29140559	None:intergenic
AACCGTGAAATGACGGAGAA+GGG	0.559698	2_1:-29140640	None:intergenic
AAACCGTGAAATGACGGAGA+AGG	0.567863	2_1:-29140641	None:intergenic
CTCTCGATCTACTCCACTGA+AGG	0.575069	2_1:+29140569	Msa0204230:five_prime_UTR
CAAATATGTCAAACATGAGA+CGG	0.588251	2_1:-29140675	None:intergenic
TTATCTAGTCAAAGCTCTAG+TGG	0.590980	2_1:-29141981	None:intergenic
GGAGACAAGAGAGCCTTCAG+TGG	0.593032	2_1:-29140582	None:intergenic
TTCATCAATGGGAAATGGAA+CGG	0.599659	2_1:+29141343	Msa0204230:CDS
AGAAACCAAACATCTTCTCA+TGG	0.615290	2_1:+29141313	Msa0204230:CDS
AGATGAGAAGGAGATAGCGA+TGG	0.617672	2_1:-29140603	None:intergenic
GCATTTGTGTTTGTGGACCG+TGG	0.648507	2_1:+29141020	Msa0204230:five_prime_UTR
TGATTGGGAAAACAAGCTTG+AGG	0.653830	2_1:+29141748	Msa0204230:CDS
TTCAGACAGAAGAAGCAGAG+CGG	0.693835	2_1:+29141444	Msa0204230:intron
TGACGGAGAAGGGAACGATG+TGG	0.700100	2_1:-29140630	None:intergenic
TCAGCCTTGACAACAAACTG+AGG	0.709180	2_1:-29141698	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTGTTTTTAATGAAA+TGG	+	chr2_1:29140801-29140820	Msa0204230:intron	10.0%
!!!	ATTTTTGTTTTTAATGAAAT+GGG	+	chr2_1:29140802-29140821	Msa0204230:intron	10.0%
!!	TAAAAATTAATATCATGAAA+GGG	-	chr2_1:29140959-29140978	None:intergenic	10.0%
!!!	ATATTAATTTTTACTTTGAT+GGG	+	chr2_1:29140966-29140985	Msa0204230:intron	10.0%
!!!	AATTTTTGTTTTTAATGAAA+TGG	+	chr2_1:29140801-29140820	Msa0204230:intron	10.0%
!!!	ATTTTTGTTTTTAATGAAAT+GGG	+	chr2_1:29140802-29140821	Msa0204230:intron	10.0%
!!	TAAAAATTAATATCATGAAA+GGG	-	chr2_1:29140959-29140978	None:intergenic	10.0%
!!!	ATATTAATTTTTACTTTGAT+GGG	+	chr2_1:29140966-29140985	Msa0204230:intron	10.0%
!!	GTAAAAATTAATATCATGAA+AGG	-	chr2_1:29140960-29140979	None:intergenic	15.0%
!!!	GATATTAATTTTTACTTTGA+TGG	+	chr2_1:29140965-29140984	Msa0204230:intron	15.0%
!!	AAAATATTATATTATCTCGT+AGG	-	chr2_1:29141190-29141209	None:intergenic	15.0%
!!!	TAATATAATATTTTTGTGAC+AGG	+	chr2_1:29141196-29141215	Msa0204230:intron	15.0%
!!	GTAAAAATTAATATCATGAA+AGG	-	chr2_1:29140960-29140979	None:intergenic	15.0%
!!!	GATATTAATTTTTACTTTGA+TGG	+	chr2_1:29140965-29140984	Msa0204230:intron	15.0%
!!	AAAATATTATATTATCTCGT+AGG	-	chr2_1:29141190-29141209	None:intergenic	15.0%
!!!	TAATATAATATTTTTGTGAC+AGG	+	chr2_1:29141196-29141215	Msa0204230:intron	15.0%
!!!	ATTTGTTGAAAATTAGAGTT+TGG	+	chr2_1:29140856-29140875	Msa0204230:intron	20.0%
!!!	TTATTTTATACTCTATCAAG+AGG	+	chr2_1:29141480-29141499	Msa0204230:CDS	20.0%
!!	GTATATAATCACAATTAACT+TGG	-	chr2_1:29141809-29141828	None:intergenic	20.0%
!!!	TAAGTATATAACTGATTTGA+AGG	-	chr2_1:29141840-29141859	None:intergenic	20.0%
!!	CACATATATTAATTCAAAAC+AGG	-	chr2_1:29141893-29141912	None:intergenic	20.0%
!!!	ATTTGTTGAAAATTAGAGTT+TGG	+	chr2_1:29140856-29140875	Msa0204230:intron	20.0%
!!!	TTATTTTATACTCTATCAAG+AGG	+	chr2_1:29141480-29141499	Msa0204230:CDS	20.0%
!!	GTATATAATCACAATTAACT+TGG	-	chr2_1:29141809-29141828	None:intergenic	20.0%
!!!	TAAGTATATAACTGATTTGA+AGG	-	chr2_1:29141840-29141859	None:intergenic	20.0%
!!	CACATATATTAATTCAAAAC+AGG	-	chr2_1:29141893-29141912	None:intergenic	20.0%
!	AATTGAACATCTCCTATATT+GGG	-	chr2_1:29140750-29140769	None:intergenic	25.0%
!	AAATTGAACATCTCCTATAT+TGG	-	chr2_1:29140751-29140770	None:intergenic	25.0%
!!!	AAATGGGTTTGTTGAAATTT+TGG	+	chr2_1:29140818-29140837	Msa0204230:intron	25.0%
!	AAAATTAGAGTTTGGTTAGT+GGG	+	chr2_1:29140864-29140883	Msa0204230:intron	25.0%
!!!	AGTTTTTATTTGAAGTCATG+AGG	+	chr2_1:29141409-29141428	Msa0204230:intron	25.0%
!!	TAGCATTATAGTTTGTAGTT+TGG	+	chr2_1:29141612-29141631	Msa0204230:intron	25.0%
!	CTAAATCGATTGCTTAAAAT+TGG	-	chr2_1:29141725-29141744	None:intergenic	25.0%
!	AATTGAACATCTCCTATATT+GGG	-	chr2_1:29140750-29140769	None:intergenic	25.0%
!	AAATTGAACATCTCCTATAT+TGG	-	chr2_1:29140751-29140770	None:intergenic	25.0%
!!!	AAATGGGTTTGTTGAAATTT+TGG	+	chr2_1:29140818-29140837	Msa0204230:intron	25.0%
!	AAAATTAGAGTTTGGTTAGT+GGG	+	chr2_1:29140864-29140883	Msa0204230:intron	25.0%
!!!	AGTTTTTATTTGAAGTCATG+AGG	+	chr2_1:29141409-29141428	Msa0204230:intron	25.0%
!!	TAGCATTATAGTTTGTAGTT+TGG	+	chr2_1:29141612-29141631	Msa0204230:intron	25.0%
!	CTAAATCGATTGCTTAAAAT+TGG	-	chr2_1:29141725-29141744	None:intergenic	25.0%
	AGAGAGAAAGTTGAATGAAT+TGG	-	chr2_1:29140492-29140511	None:intergenic	30.0%
	CAAATATGTCAAACATGAGA+CGG	-	chr2_1:29140678-29140697	None:intergenic	30.0%
!!!	TATTTGTTTTGTGCAATCTC+AGG	+	chr2_1:29140692-29140711	Msa0204230:five_prime_UTR	30.0%
	ATTGAACATCTCCTATATTG+GGG	-	chr2_1:29140749-29140768	None:intergenic	30.0%
	GAAAATTAGAGTTTGGTTAG+TGG	+	chr2_1:29140863-29140882	Msa0204230:intron	30.0%
	AGAAAACTGTAAAGAAGCAA+TGG	+	chr2_1:29141280-29141299	Msa0204230:exon	30.0%
	ATGGCAAATGTTTCATCAAT+GGG	+	chr2_1:29141332-29141351	Msa0204230:CDS	30.0%
	AATGTTTCATCAATGGGAAA+TGG	+	chr2_1:29141338-29141357	Msa0204230:CDS	30.0%
	AAAGATGAGATTGAGAATAC+TGG	+	chr2_1:29141530-29141549	Msa0204230:CDS	30.0%
	CACAAGCAAACATAAAAGAT+CGG	-	chr2_1:29141864-29141883	None:intergenic	30.0%
!	ATCTTTTATGTTTGCTTGTG+CGG	+	chr2_1:29141864-29141883	Msa0204230:intron	30.0%
	AGAGAGAAAGTTGAATGAAT+TGG	-	chr2_1:29140492-29140511	None:intergenic	30.0%
	CAAATATGTCAAACATGAGA+CGG	-	chr2_1:29140678-29140697	None:intergenic	30.0%
!!!	TATTTGTTTTGTGCAATCTC+AGG	+	chr2_1:29140692-29140711	Msa0204230:five_prime_UTR	30.0%
	ATTGAACATCTCCTATATTG+GGG	-	chr2_1:29140749-29140768	None:intergenic	30.0%
	GAAAATTAGAGTTTGGTTAG+TGG	+	chr2_1:29140863-29140882	Msa0204230:intron	30.0%
	AGAAAACTGTAAAGAAGCAA+TGG	+	chr2_1:29141280-29141299	Msa0204230:exon	30.0%
	ATGGCAAATGTTTCATCAAT+GGG	+	chr2_1:29141332-29141351	Msa0204230:CDS	30.0%
	AATGTTTCATCAATGGGAAA+TGG	+	chr2_1:29141338-29141357	Msa0204230:CDS	30.0%
	AAAGATGAGATTGAGAATAC+TGG	+	chr2_1:29141530-29141549	Msa0204230:CDS	30.0%
	CACAAGCAAACATAAAAGAT+CGG	-	chr2_1:29141864-29141883	None:intergenic	30.0%
!	ATCTTTTATGTTTGCTTGTG+CGG	+	chr2_1:29141864-29141883	Msa0204230:intron	30.0%
	ACGAATAAACCGTGAAATGA+CGG	-	chr2_1:29140650-29140669	None:intergenic	35.0%
	ATAGACTGCATTTGTGTTTG+TGG	+	chr2_1:29141013-29141032	Msa0204230:five_prime_UTR	35.0%
	CACAAAACTGAAACACCAAA+AGG	-	chr2_1:29141136-29141155	None:intergenic	35.0%
!!	TGTTTCAGTTTTGTGCTGTT+AGG	+	chr2_1:29141141-29141160	Msa0204230:five_prime_UTR	35.0%
!!	TTTGTGCTGTTAGGTTGTTT+TGG	+	chr2_1:29141150-29141169	Msa0204230:intron	35.0%
	AAAGAAGCAATGGATCTGTT+TGG	+	chr2_1:29141290-29141309	Msa0204230:exon	35.0%
	AGAAACCAAACATCTTCTCA+TGG	+	chr2_1:29141313-29141332	Msa0204230:CDS	35.0%
	ATTTGCCATGAGAAGATGTT+TGG	-	chr2_1:29141321-29141340	None:intergenic	35.0%
	CATGGCAAATGTTTCATCAA+TGG	+	chr2_1:29141331-29141350	Msa0204230:CDS	35.0%
	TTCATCAATGGGAAATGGAA+CGG	+	chr2_1:29141343-29141362	Msa0204230:CDS	35.0%
!!	CAATCGATTTAGCTCTGATT+GGG	+	chr2_1:29141733-29141752	Msa0204230:CDS	35.0%
!	TCTTTTATGTTTGCTTGTGC+GGG	+	chr2_1:29141865-29141884	Msa0204230:intron	35.0%
	TGAACAGAGTAACGTCAATA+AGG	+	chr2_1:29141926-29141945	Msa0204230:intron	35.0%
	ACGAATAAACCGTGAAATGA+CGG	-	chr2_1:29140650-29140669	None:intergenic	35.0%
	ATAGACTGCATTTGTGTTTG+TGG	+	chr2_1:29141013-29141032	Msa0204230:five_prime_UTR	35.0%
	CACAAAACTGAAACACCAAA+AGG	-	chr2_1:29141136-29141155	None:intergenic	35.0%
!!	TGTTTCAGTTTTGTGCTGTT+AGG	+	chr2_1:29141141-29141160	Msa0204230:five_prime_UTR	35.0%
!!	TTTGTGCTGTTAGGTTGTTT+TGG	+	chr2_1:29141150-29141169	Msa0204230:intron	35.0%
	AAAGAAGCAATGGATCTGTT+TGG	+	chr2_1:29141290-29141309	Msa0204230:exon	35.0%
	AGAAACCAAACATCTTCTCA+TGG	+	chr2_1:29141313-29141332	Msa0204230:CDS	35.0%
	ATTTGCCATGAGAAGATGTT+TGG	-	chr2_1:29141321-29141340	None:intergenic	35.0%
	CATGGCAAATGTTTCATCAA+TGG	+	chr2_1:29141331-29141350	Msa0204230:CDS	35.0%
	TTCATCAATGGGAAATGGAA+CGG	+	chr2_1:29141343-29141362	Msa0204230:CDS	35.0%
!!	CAATCGATTTAGCTCTGATT+GGG	+	chr2_1:29141733-29141752	Msa0204230:CDS	35.0%
!	TCTTTTATGTTTGCTTGTGC+GGG	+	chr2_1:29141865-29141884	Msa0204230:intron	35.0%
	TGAACAGAGTAACGTCAATA+AGG	+	chr2_1:29141926-29141945	Msa0204230:intron	35.0%
	GGGGATATTAGGGTTTCAAT+GGG	-	chr2_1:29140534-29140553	None:intergenic	40.0%
	TGGGGATATTAGGGTTTCAA+TGG	-	chr2_1:29140535-29140554	None:intergenic	40.0%
	GATGGAAGTTGGGGATATTA+GGG	-	chr2_1:29140544-29140563	None:intergenic	40.0%
	AGATGGAAGTTGGGGATATT+AGG	-	chr2_1:29140545-29140564	None:intergenic	40.0%
	GGAACAAAGCTGACAAATAG+AGG	-	chr2_1:29140728-29140747	None:intergenic	40.0%
!!	TCAGCTTTGTTCCCCAATAT+AGG	+	chr2_1:29140735-29140754	Msa0204230:intron	40.0%
!!	TTTACTTTGATGGGTTGCTC+AGG	+	chr2_1:29140975-29140994	Msa0204230:intron	40.0%
	AAAACACCAAAAACCTGCCA+CGG	-	chr2_1:29141040-29141059	None:intergenic	40.0%
!!!	GCAGGTTTTTGGTGTTTTAG+TGG	+	chr2_1:29141042-29141061	Msa0204230:five_prime_UTR	40.0%
!	CTTTTGATGCTTCTGCTGTT+TGG	+	chr2_1:29141110-29141129	Msa0204230:five_prime_UTR	40.0%
	TGTTCTCAGTATTCGACACA+AGG	-	chr2_1:29141561-29141580	None:intergenic	40.0%
!!	ACTTCCTCAGTTTGTTGTCA+AGG	+	chr2_1:29141694-29141713	Msa0204230:CDS	40.0%
!!	GCAATCGATTTAGCTCTGAT+TGG	+	chr2_1:29141732-29141751	Msa0204230:CDS	40.0%
	TGATTGGGAAAACAAGCTTG+AGG	+	chr2_1:29141748-29141767	Msa0204230:CDS	40.0%
!	CTTTTATGTTTGCTTGTGCG+GGG	+	chr2_1:29141866-29141885	Msa0204230:intron	40.0%
!	TTTTATGTTTGCTTGTGCGG+GGG	+	chr2_1:29141867-29141886	Msa0204230:intron	40.0%
	GGGGATATTAGGGTTTCAAT+GGG	-	chr2_1:29140534-29140553	None:intergenic	40.0%
	TGGGGATATTAGGGTTTCAA+TGG	-	chr2_1:29140535-29140554	None:intergenic	40.0%
	GATGGAAGTTGGGGATATTA+GGG	-	chr2_1:29140544-29140563	None:intergenic	40.0%
	AGATGGAAGTTGGGGATATT+AGG	-	chr2_1:29140545-29140564	None:intergenic	40.0%
	GGAACAAAGCTGACAAATAG+AGG	-	chr2_1:29140728-29140747	None:intergenic	40.0%
!!	TCAGCTTTGTTCCCCAATAT+AGG	+	chr2_1:29140735-29140754	Msa0204230:intron	40.0%
!!	TTTACTTTGATGGGTTGCTC+AGG	+	chr2_1:29140975-29140994	Msa0204230:intron	40.0%
	AAAACACCAAAAACCTGCCA+CGG	-	chr2_1:29141040-29141059	None:intergenic	40.0%
!!!	GCAGGTTTTTGGTGTTTTAG+TGG	+	chr2_1:29141042-29141061	Msa0204230:five_prime_UTR	40.0%
!	CTTTTGATGCTTCTGCTGTT+TGG	+	chr2_1:29141110-29141129	Msa0204230:five_prime_UTR	40.0%
	TGTTCTCAGTATTCGACACA+AGG	-	chr2_1:29141561-29141580	None:intergenic	40.0%
!!	ACTTCCTCAGTTTGTTGTCA+AGG	+	chr2_1:29141694-29141713	Msa0204230:CDS	40.0%
!!	GCAATCGATTTAGCTCTGAT+TGG	+	chr2_1:29141732-29141751	Msa0204230:CDS	40.0%
	TGATTGGGAAAACAAGCTTG+AGG	+	chr2_1:29141748-29141767	Msa0204230:CDS	40.0%
!	CTTTTATGTTTGCTTGTGCG+GGG	+	chr2_1:29141866-29141885	Msa0204230:intron	40.0%
!	TTTTATGTTTGCTTGTGCGG+GGG	+	chr2_1:29141867-29141886	Msa0204230:intron	40.0%
	AGAGAGTGAGATGGAAGTTG+GGG	-	chr2_1:29140553-29140572	None:intergenic	45.0%
	GAGAGAGTGAGATGGAAGTT+GGG	-	chr2_1:29140554-29140573	None:intergenic	45.0%
	AGTAGATCGAGAGAGTGAGA+TGG	-	chr2_1:29140562-29140581	None:intergenic	45.0%
	AGATGAGAAGGAGATAGCGA+TGG	-	chr2_1:29140606-29140625	None:intergenic	45.0%
	TTCCCTTCTCCGTCATTTCA+CGG	+	chr2_1:29140638-29140657	Msa0204230:five_prime_UTR	45.0%
!	AACCGTGAAATGACGGAGAA+GGG	-	chr2_1:29140643-29140662	None:intergenic	45.0%
!	AAACCGTGAAATGACGGAGA+AGG	-	chr2_1:29140644-29140663	None:intergenic	45.0%
	GTTGCTCAGGACGTAATTTG+AGG	+	chr2_1:29140988-29141007	Msa0204230:intron	45.0%
	TTCAGACAGAAGAAGCAGAG+CGG	+	chr2_1:29141444-29141463	Msa0204230:intron	45.0%
!	GAGGCTGACTCAGAAAAATG+AGG	+	chr2_1:29141499-29141518	Msa0204230:CDS	45.0%
	TCAGCCTTGACAACAAACTG+AGG	-	chr2_1:29141701-29141720	None:intergenic	45.0%
	AGAGAGTGAGATGGAAGTTG+GGG	-	chr2_1:29140553-29140572	None:intergenic	45.0%
	GAGAGAGTGAGATGGAAGTT+GGG	-	chr2_1:29140554-29140573	None:intergenic	45.0%
	AGTAGATCGAGAGAGTGAGA+TGG	-	chr2_1:29140562-29140581	None:intergenic	45.0%
	AGATGAGAAGGAGATAGCGA+TGG	-	chr2_1:29140606-29140625	None:intergenic	45.0%
	TTCCCTTCTCCGTCATTTCA+CGG	+	chr2_1:29140638-29140657	Msa0204230:five_prime_UTR	45.0%
!	AACCGTGAAATGACGGAGAA+GGG	-	chr2_1:29140643-29140662	None:intergenic	45.0%
!	AAACCGTGAAATGACGGAGA+AGG	-	chr2_1:29140644-29140663	None:intergenic	45.0%
	GTTGCTCAGGACGTAATTTG+AGG	+	chr2_1:29140988-29141007	Msa0204230:intron	45.0%
	TTCAGACAGAAGAAGCAGAG+CGG	+	chr2_1:29141444-29141463	Msa0204230:intron	45.0%
!	GAGGCTGACTCAGAAAAATG+AGG	+	chr2_1:29141499-29141518	Msa0204230:CDS	45.0%
	TCAGCCTTGACAACAAACTG+AGG	-	chr2_1:29141701-29141720	None:intergenic	45.0%
	CGAGAGAGTGAGATGGAAGT+TGG	-	chr2_1:29140555-29140574	None:intergenic	50.0%
	CTCTCGATCTACTCCACTGA+AGG	+	chr2_1:29140569-29140588	Msa0204230:five_prime_UTR	50.0%
	CGATGTGGTCAGAGATGAGA+AGG	-	chr2_1:29140618-29140637	None:intergenic	50.0%
	GCATTTGTGTTTGTGGACCG+TGG	+	chr2_1:29141020-29141039	Msa0204230:five_prime_UTR	50.0%
	CTGCTGTTATAGCTCTCGAC+TGG	+	chr2_1:29141087-29141106	Msa0204230:five_prime_UTR	50.0%
!!	GCTTCTGCTGTTTGGCCTTT+TGG	+	chr2_1:29141118-29141137	Msa0204230:five_prime_UTR	50.0%
	GCAGCTGAATGAGCTAGCAT+TGG	+	chr2_1:29141254-29141273	Msa0204230:intron	50.0%
	CGAGAGAGTGAGATGGAAGT+TGG	-	chr2_1:29140555-29140574	None:intergenic	50.0%
	CTCTCGATCTACTCCACTGA+AGG	+	chr2_1:29140569-29140588	Msa0204230:five_prime_UTR	50.0%
	CGATGTGGTCAGAGATGAGA+AGG	-	chr2_1:29140618-29140637	None:intergenic	50.0%
	GCATTTGTGTTTGTGGACCG+TGG	+	chr2_1:29141020-29141039	Msa0204230:five_prime_UTR	50.0%
	CTGCTGTTATAGCTCTCGAC+TGG	+	chr2_1:29141087-29141106	Msa0204230:five_prime_UTR	50.0%
!!	GCTTCTGCTGTTTGGCCTTT+TGG	+	chr2_1:29141118-29141137	Msa0204230:five_prime_UTR	50.0%
	GCAGCTGAATGAGCTAGCAT+TGG	+	chr2_1:29141254-29141273	Msa0204230:intron	50.0%
	GGAGACAAGAGAGCCTTCAG+TGG	-	chr2_1:29140585-29140604	None:intergenic	55.0%
	TGACGGAGAAGGGAACGATG+TGG	-	chr2_1:29140633-29140652	None:intergenic	55.0%
	TTGTGTTTGTGGACCGTGGC+AGG	+	chr2_1:29141024-29141043	Msa0204230:five_prime_UTR	55.0%
!	TGTGGACCGTGGCAGGTTTT+TGG	+	chr2_1:29141031-29141050	Msa0204230:five_prime_UTR	55.0%
	GGAGACAAGAGAGCCTTCAG+TGG	-	chr2_1:29140585-29140604	None:intergenic	55.0%
	TGACGGAGAAGGGAACGATG+TGG	-	chr2_1:29140633-29140652	None:intergenic	55.0%
	TTGTGTTTGTGGACCGTGGC+AGG	+	chr2_1:29141024-29141043	Msa0204230:five_prime_UTR	55.0%
!	TGTGGACCGTGGCAGGTTTT+TGG	+	chr2_1:29141031-29141050	Msa0204230:five_prime_UTR	55.0%
	CAGAAGAAGCAGAGCGGTGC+TGG	+	chr2_1:29141450-29141469	Msa0204230:CDS	60.0%
	CAGAAGAAGCAGAGCGGTGC+TGG	+	chr2_1:29141450-29141469	Msa0204230:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	29140487	29141995	29140487	ID=Msa0204230;Name=Msa0204230
chr2_1	mRNA	29140487	29141995	29140487	ID=Msa0204230-mRNA-1;Parent=Msa0204230;Name=Msa0204230-mRNA-1;_AED=0.39;_eAED=0.39;_QI=431\|0.6\|0.66\|0.83\|0.6\|0.5\|6\|0\|106
chr2_1	exon	29140487	29140713	29140487	ID=Msa0204230-mRNA-1:exon:10439;Parent=Msa0204230-mRNA-1
chr2_1	exon	29140997	29141162	29140997	ID=Msa0204230-mRNA-1:exon:10440;Parent=Msa0204230-mRNA-1
chr2_1	exon	29141261	29141364	29141261	ID=Msa0204230-mRNA-1:exon:10441;Parent=Msa0204230-mRNA-1
chr2_1	exon	29141449	29141551	29141449	ID=Msa0204230-mRNA-1:exon:10442;Parent=Msa0204230-mRNA-1
chr2_1	exon	29141681	29141769	29141681	ID=Msa0204230-mRNA-1:exon:10443;Parent=Msa0204230-mRNA-1
chr2_1	exon	29141933	29141995	29141933	ID=Msa0204230-mRNA-1:exon:10444;Parent=Msa0204230-mRNA-1
chr2_1	five_prime_UTR	29140487	29140713	29140487	ID=Msa0204230-mRNA-1:five_prime_utr;Parent=Msa0204230-mRNA-1
chr2_1	five_prime_UTR	29140997	29141162	29140997	ID=Msa0204230-mRNA-1:five_prime_utr;Parent=Msa0204230-mRNA-1
chr2_1	five_prime_UTR	29141261	29141298	29141261	ID=Msa0204230-mRNA-1:five_prime_utr;Parent=Msa0204230-mRNA-1
chr2_1	CDS	29141299	29141364	29141299	ID=Msa0204230-mRNA-1:cds;Parent=Msa0204230-mRNA-1
chr2_1	CDS	29141449	29141551	29141449	ID=Msa0204230-mRNA-1:cds;Parent=Msa0204230-mRNA-1
chr2_1	CDS	29141681	29141769	29141681	ID=Msa0204230-mRNA-1:cds;Parent=Msa0204230-mRNA-1
chr2_1	CDS	29141933	29141995	29141933	ID=Msa0204230-mRNA-1:cds;Parent=Msa0204230-mRNA-1

Gene Sequence	>Msa0204230 ATGGATCTGTTTGGAGAAACCAAACATCTTCTCATGGCAAATGTTTCATCAATGGGAAATGGAACGACAGAAGAAGCAGAGCGGTGCTGGTTTGCTTTTATTTTATACTCTATCAAGAGGCTGACTCAGAAAAATGAGGAGAGTGAAAAAGATGAGATTGAGAATACTGATTTTTTCAAAGAACTTCCTCAGTTTGTTGTCAAGGCTGATCCAATTTTAAGCAATCGATTTAGCTCTGATTGGGAAAACAAGCTTGAGAGTAACGTCAATAAGGTTTTGAACAGAGTAACATCAATAACAATATTACCACTAGAGCTTTGA
Protein sequence	>Msa0204230 MDLFGETKHLLMANVSSMGNGTTEEAERCWFAFILYSIKRLTQKNEESEKDEIENTDFFKELPQFVVKADPILSNRFSSDWENKLESNVNKVLNRVTSITILPLEL*