AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1319490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1319490 MtrunA17_Chr8g0353751 81.319 91 5 1 1 79 46 136 1.31e-32 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1319490 AT3G12280.2 45.652 92 32 2 1 77 42 130 2.42e-17 75.1
Msa1319490 AT3G12280.1 45.652 92 32 2 1 77 42 130 2.42e-17 75.1

Find 87 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATATATGTTGTTATTT+TGG 0.157057 8_4:-22708930 Msa1319490:three_prime_UTR
CTAAATCAATTGCTTAAAAT+TGG 0.181934 8_4:+22710393 None:intergenic
AGATGGAAGTTGGGGATATT+AGG 0.184788 8_4:+22711572 None:intergenic
GCTTCTGCTGTTTGGCCTTT+TGG 0.186951 8_4:-22710997 Msa1319490:five_prime_UTR
TTACTAGAACTTTATCCTTT+TGG 0.247208 8_4:-22708968 Msa1319490:three_prime_UTR
CCCAACTAATGACACAAATT+TGG 0.248709 8_4:-22709058 Msa1319490:three_prime_UTR
TAAATCTTTCTCCTTTCTAT+TGG 0.265278 8_4:+22709932 None:intergenic
TTGTCAATTTGTTCAACTTT+AGG 0.267025 8_4:+22709123 None:intergenic
AACATAAGCACAAATAGATT+TGG 0.270375 8_4:+22709673 None:intergenic
CGAGAGAGTGAGATGGAAGT+TGG 0.292056 8_4:+22711562 None:intergenic
AAATGAAATGTAATGAAAAT+AGG 0.297485 8_4:-22709504 Msa1319490:three_prime_UTR
TCCAAATTTGTGTCATTAGT+TGG 0.309488 8_4:+22709057 None:intergenic
ACATAAGCACAAATAGATTT+GGG 0.318687 8_4:+22709674 None:intergenic
TTCCCTTCTCCGTCATTTCA+CGG 0.319534 8_4:-22711476 Msa1319490:five_prime_UTR
TAAATTATATAACAAAATTA+TGG 0.320560 8_4:+22709439 None:intergenic
GATGGAAGTTGGGGATATTA+GGG 0.321992 8_4:+22711573 None:intergenic
TTTGTATTATCACGTTCTTT+AGG 0.327327 8_4:+22709258 None:intergenic
AGAGAGAAAGTTGAATGAAT+TGG 0.333762 8_4:+22711625 None:intergenic
TAGCTAAACACGACCGTTGA+TGG 0.336469 8_4:-22709897 Msa1319490:three_prime_UTR
TGAGAACATGCAAGTGTTAC+CGG 0.341443 8_4:-22709151 Msa1319490:three_prime_UTR
CCAAATTTGTGTCATTAGTT+GGG 0.352428 8_4:+22709058 None:intergenic
GCAATTGATTTAGCTCTGAT+TGG 0.356685 8_4:-22710383 Msa1319490:exon
AGATACGACACTATGAGTTT+TGG 0.358379 8_4:-22709625 Msa1319490:three_prime_UTR
TTGTGGACCGTGGCAGGTTT+TGG 0.358608 8_4:-22711084 Msa1319490:five_prime_UTR
ATGTGTAGGAGGTTTATATT+TGG 0.360770 8_4:-22709721 Msa1319490:three_prime_UTR
CATGGCAAATGTTTCATCAA+TGG 0.366338 8_4:-22710784 Msa1319490:CDS
GAGAGAGTGAGATGGAAGTT+GGG 0.366637 8_4:+22711563 None:intergenic
GTAAATTATATAGAAGTTGT+AGG 0.376919 8_4:-22709574 Msa1319490:three_prime_UTR
ATTTGCCATGAGAAGATGTT+TGG 0.379727 8_4:+22710797 None:intergenic
TTAGACCTGCCATTTCAAAA+AGG 0.384281 8_4:-22709384 Msa1319490:three_prime_UTR
AAGAACGTGATAATACAAAT+TGG 0.384601 8_4:-22709254 Msa1319490:three_prime_UTR
TTCACTATTTATGTCATGAA+TGG 0.398659 8_4:+22709538 None:intergenic
AAAGATGAGATTGAGAATAC+TGG 0.403583 8_4:-22710585 Msa1319490:CDS
CAGAAGAAGCAGAGCGGTGC+TGG 0.404107 8_4:-22710665 Msa1319490:CDS
AATGTTTCATCAATGGGAAA+TGG 0.408483 8_4:-22710777 Msa1319490:CDS
TGGGGATATTAGGGTTTCAA+TGG 0.409813 8_4:+22711582 None:intergenic
TGTTCAACTTTAGGAGTAAC+CGG 0.415342 8_4:+22709132 None:intergenic
AAAGAAGCAATGGATCTGTT+TGG 0.423829 8_4:-22710825 Msa1319490:exon
CGGTCGTGTTTAGCTAGATT+AGG 0.423966 8_4:+22709904 None:intergenic
ATGGCAAATGTTTCATCAAT+GGG 0.427540 8_4:-22710783 Msa1319490:CDS
GTTCAACTATGATGATGAAA+TGG 0.434720 8_4:-22709026 Msa1319490:three_prime_UTR
ATAGACTGCATTTGTGTTTG+TGG 0.436210 8_4:-22711101 Msa1319490:five_prime_UTR
TTGTGTTTGTGGACCGTGGC+AGG 0.441265 8_4:-22711090 Msa1319490:five_prime_UTR
GACACAAATTTGGAAGATGA+AGG 0.468448 8_4:-22709048 Msa1319490:three_prime_UTR
CAATTGATTTAGCTCTGATT+GGG 0.469143 8_4:-22710382 Msa1319490:exon
CTGCTGTTATAGCTCTCGAC+TGG 0.487878 8_4:-22711028 Msa1319490:five_prime_UTR
ACTTCCTCAGTTTGTTGTCA+AGG 0.489779 8_4:-22710421 Msa1319490:CDS
AAATGTTGTGAGCTACAATC+TGG 0.491307 8_4:-22709286 Msa1319490:three_prime_UTR
ACGAATAAACCGTGAAATGA+CGG 0.502367 8_4:+22711467 None:intergenic
TAAATTATATAGAAGTTGTA+GGG 0.503058 8_4:-22709573 Msa1319490:three_prime_UTR
TATAATTTACAAGTTTGTCT+AGG 0.510316 8_4:-22709425 Msa1319490:three_prime_UTR
TCCTTTCTATTGGATTTAGA+CGG 0.512168 8_4:+22709942 None:intergenic
GTTGCTCAGGACGTAATTTG+AGG 0.514531 8_4:-22711126 Msa1319490:intron
ACCGTCTAAATCCAATAGAA+AGG 0.518114 8_4:-22709943 Msa1319490:three_prime_UTR
GGGGATATTAGGGTTTCAAT+GGG 0.524270 8_4:+22711583 None:intergenic
GGGTGTTTAGTATGTGTAGG+AGG 0.528912 8_4:-22709732 Msa1319490:three_prime_UTR
CGATGTGGTCAGAGATGAGA+AGG 0.536025 8_4:+22711499 None:intergenic
TGATAATACAAATTGGTTGG+TGG 0.537737 8_4:-22709247 Msa1319490:three_prime_UTR
AGAAAACTGTAAAGAAGCAA+TGG 0.539140 8_4:-22710835 Msa1319490:exon
AGAGAGTGAGATGGAAGTTG+GGG 0.540686 8_4:+22711564 None:intergenic
CACAAAACTGAAACACCAAA+AGG 0.551426 8_4:+22710982 None:intergenic
GCAGCTGAATGAGCTAGCAT+TGG 0.552255 8_4:-22710861 Msa1319490:intron
AGTAGATCGAGAGAGTGAGA+TGG 0.559499 8_4:+22711555 None:intergenic
AACCGTGAAATGACGGAGAA+GGG 0.559698 8_4:+22711474 None:intergenic
CTTGGGTGTTTAGTATGTGT+AGG 0.561793 8_4:-22709735 Msa1319490:three_prime_UTR
GGGTTTAGAGCTTCCATCAA+CGG 0.561850 8_4:+22709884 None:intergenic
AAACCGTGAAATGACGGAGA+AGG 0.567863 8_4:+22711473 None:intergenic
CTCTCGATCTACTCCACTGA+AGG 0.575069 8_4:-22711545 Msa1319490:five_prime_UTR
CAAATATGTCAAACATGAGA+CGG 0.588251 8_4:+22711439 None:intergenic
ACGTGATAATACAAATTGGT+TGG 0.591902 8_4:-22709250 Msa1319490:three_prime_UTR
GGAGACAAGAGAGCCTTCAG+TGG 0.593032 8_4:+22711532 None:intergenic
TTCATCAATGGGAAATGGAA+CGG 0.599659 8_4:-22710772 Msa1319490:intron
TAAAACACCAAAACCTGCCA+CGG 0.601138 8_4:+22711077 None:intergenic
AGAAACCAAACATCTTCTCA+TGG 0.615290 8_4:-22710802 Msa1319490:CDS
ATAAGCACAAATAGATTTGG+GGG 0.617177 8_4:+22709676 None:intergenic
AGATGAGAAGGAGATAGCGA+TGG 0.617672 8_4:+22711511 None:intergenic
CATAAGCACAAATAGATTTG+GGG 0.621352 8_4:+22709675 None:intergenic
GTTGGTGGTTATCATGACAA+AGG 0.626169 8_4:-22709232 Msa1319490:three_prime_UTR
GCATTTGTGTTTGTGGACCG+TGG 0.648507 8_4:-22711094 Msa1319490:five_prime_UTR
TGATTGGGAAAACAAGCTTG+AGG 0.653830 8_4:-22710367 Msa1319490:intron
TAAGCACAAATAGATTTGGG+GGG 0.671507 8_4:+22709677 None:intergenic
AATAGGTGAACTGACTGCAA+TGG 0.672632 8_4:-22709487 Msa1319490:three_prime_UTR
TTGATGGAAGCTCTAAACCC+AGG 0.677318 8_4:-22709881 Msa1319490:three_prime_UTR
TTCAGACAGAAGAAGCAGAG+CGG 0.693835 8_4:-22710671 Msa1319490:intron
AAGCACAAATAGATTTGGGG+GGG 0.699974 8_4:+22709678 None:intergenic
TGACGGAGAAGGGAACGATG+TGG 0.700100 8_4:+22711484 None:intergenic
TCAGCCTTGACAACAAACTG+AGG 0.709180 8_4:+22710417 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTGTTTTTAATGAAA+TGG - chr8_4:22709184-22709203 Msa1319490:three_prime_UTR 10.0%
!!! ATTTTTGTTTTTAATGAAAT+GGG - chr8_4:22709185-22709204 Msa1319490:three_prime_UTR 10.0%
!! TAAAAATTAATATCATGAAA+GGG + chr8_4:22709342-22709361 None:intergenic 10.0%
!!! ATATTAATTTTTACTTTGAT+GGG - chr8_4:22709349-22709368 Msa1319490:three_prime_UTR 10.0%
!! AAAAATTATCATAATAAAAG+AGG + chr8_4:22710672-22710691 None:intergenic 10.0%
!! GTAAAAATTAATATCATGAA+AGG + chr8_4:22709343-22709362 None:intergenic 15.0%
!!! GATATTAATTTTTACTTTGA+TGG - chr8_4:22709348-22709367 Msa1319490:three_prime_UTR 15.0%
!! AAAATATTATATTATCTCGT+AGG + chr8_4:22709572-22709591 None:intergenic 15.0%
!!! TAATATAATATTTTTGTGAC+AGG - chr8_4:22709578-22709597 Msa1319490:three_prime_UTR 15.0%
!! AATAAACAAACAATCAATAT+TGG + chr8_4:22710460-22710479 None:intergenic 15.0%
!! TAAATTATATAGAAGTTGTA+GGG - chr8_4:22710924-22710943 Msa1319490:intron 15.0%
!!! TATTTTCATTACATTTCATT+TGG + chr8_4:22710994-22711013 None:intergenic 15.0%
!! AAATGAAATGTAATGAAAAT+AGG - chr8_4:22710993-22711012 Msa1319490:five_prime_UTR 15.0%
!!! TTTGATATATGTTGTTATTT+TGG - chr8_4:22711567-22711586 Msa1319490:five_prime_UTR 15.0%
!!! ATTTGTTGAAAATTAGAGTT+TGG - chr8_4:22709239-22709258 Msa1319490:three_prime_UTR 20.0%
!!! TTATTTTATACTCTATCAAG+AGG - chr8_4:22709862-22709881 Msa1319490:three_prime_UTR 20.0%
!! CTAAATCAATTGCTTAAAAT+TGG + chr8_4:22710107-22710126 None:intergenic 20.0%
!! GTATATAATCACAATTAACT+TGG + chr8_4:22710191-22710210 None:intergenic 20.0%
!!! TAAGTATATAACTGATTTGA+AGG + chr8_4:22710222-22710241 None:intergenic 20.0%
!! CACATATATTAATTCAAAAC+AGG + chr8_4:22710275-22710294 None:intergenic 20.0%
!! GTAAATTATATAGAAGTTGT+AGG - chr8_4:22710923-22710942 Msa1319490:intron 20.0%
!! TATAATTTACAAGTTTGTCT+AGG - chr8_4:22711072-22711091 Msa1319490:five_prime_UTR 20.0%
!!! ATATATGTTGTTATTTTGGA+TGG - chr8_4:22711571-22711590 Msa1319490:five_prime_UTR 20.0%
! AATTGAACATCTCCTATATT+GGG + chr8_4:22709133-22709152 None:intergenic 25.0%
! AAATTGAACATCTCCTATAT+TGG + chr8_4:22709134-22709153 None:intergenic 25.0%
!!! AAATGGGTTTGTTGAAATTT+TGG - chr8_4:22709201-22709220 Msa1319490:three_prime_UTR 25.0%
! AAAATTAGAGTTTGGTTAGT+GGG - chr8_4:22709247-22709266 Msa1319490:three_prime_UTR 25.0%
!!! AGTTTTTATTTGAAGTCATG+AGG - chr8_4:22709791-22709810 Msa1319490:three_prime_UTR 25.0%
!! TAAAATGGTAGCATTGAATA+TGG - chr8_4:22710383-22710402 Msa1319490:exon 25.0%
!! AAAATGGTAGCATTGAATAT+GGG - chr8_4:22710384-22710403 Msa1319490:exon 25.0%
! TAAATCTTTCTCCTTTCTAT+TGG + chr8_4:22710568-22710587 None:intergenic 25.0%
! ACATAAGCACAAATAGATTT+GGG + chr8_4:22710826-22710845 None:intergenic 25.0%
! AACATAAGCACAAATAGATT+TGG + chr8_4:22710827-22710846 None:intergenic 25.0%
! TTCACTATTTATGTCATGAA+TGG + chr8_4:22710962-22710981 None:intergenic 25.0%
!!! AACTATCATCCTTTTTGAAA+TGG + chr8_4:22711125-22711144 None:intergenic 25.0%
! TTTGTATTATCACGTTCTTT+AGG + chr8_4:22711242-22711261 None:intergenic 25.0%
! AAGAACGTGATAATACAAAT+TGG - chr8_4:22711243-22711262 Msa1319490:intron 25.0%
!! TTGTCAATTTGTTCAACTTT+AGG + chr8_4:22711377-22711396 None:intergenic 25.0%
!! TTACTAGAACTTTATCCTTT+TGG - chr8_4:22711529-22711548 Msa1319490:five_prime_UTR 25.0%
!! TACTAGAACTTTATCCTTTT+GGG - chr8_4:22711530-22711549 Msa1319490:five_prime_UTR 25.0%
AGAGAGAAAGTTGAATGAAT+TGG + chr8_4:22708875-22708894 None:intergenic 30.0%
CAAATATGTCAAACATGAGA+CGG + chr8_4:22709061-22709080 None:intergenic 30.0%
!!! TATTTGTTTTGTGCAATCTC+AGG - chr8_4:22709075-22709094 Msa1319490:three_prime_UTR 30.0%
ATTGAACATCTCCTATATTG+GGG + chr8_4:22709132-22709151 None:intergenic 30.0%
GAAAATTAGAGTTTGGTTAG+TGG - chr8_4:22709246-22709265 Msa1319490:three_prime_UTR 30.0%
AGAAAACTGTAAAGAAGCAA+TGG - chr8_4:22709662-22709681 Msa1319490:three_prime_UTR 30.0%
ATGGCAAATGTTTCATCAAT+GGG - chr8_4:22709714-22709733 Msa1319490:three_prime_UTR 30.0%
AATGTTTCATCAATGGGAAA+TGG - chr8_4:22709720-22709739 Msa1319490:three_prime_UTR 30.0%
AAAGATGAGATTGAGAATAC+TGG - chr8_4:22709912-22709931 Msa1319490:three_prime_UTR 30.0%
TAGCATTAGAGTTTGTAGTT+TGG - chr8_4:22709994-22710013 Msa1319490:intron 30.0%
!! CAATTGATTTAGCTCTGATT+GGG - chr8_4:22710115-22710134 Msa1319490:intron 30.0%
CACAAGCAAACATAAAAGAT+CGG + chr8_4:22710246-22710265 None:intergenic 30.0%
! ATCTTTTATGTTTGCTTGTG+CGG - chr8_4:22710246-22710265 Msa1319490:intron 30.0%
! AGAGCTTTGACTAGATAAAA+TGG - chr8_4:22710368-22710387 Msa1319490:three_prime_UTR 30.0%
GGTTGTACAATGATGAATTT+GGG - chr8_4:22710405-22710424 Msa1319490:CDS 30.0%
CGAAAACAAACGAAAATTAG+TGG + chr8_4:22710513-22710532 None:intergenic 30.0%
!!! TTTCGTTTGTTTTGAGTTCA+AGG - chr8_4:22710528-22710547 Msa1319490:intron 30.0%
TCCTTTCTATTGGATTTAGA+CGG + chr8_4:22710558-22710577 None:intergenic 30.0%
TGAAAAAAAGTTGAAGACCT+GGG + chr8_4:22710636-22710655 None:intergenic 30.0%
ATGAAAAAAAGTTGAAGACC+TGG + chr8_4:22710637-22710656 None:intergenic 30.0%
!!! TGCATTTTTTTCAAGTGTCT+TGG - chr8_4:22710744-22710763 Msa1319490:intron 30.0%
!!! GCATTTTTTTCAAGTGTCTT+GGG - chr8_4:22710745-22710764 Msa1319490:intron 30.0%
ATGTGTAGGAGGTTTATATT+TGG - chr8_4:22710776-22710795 Msa1319490:CDS 30.0%
ATAAGCACAAATAGATTTGG+GGG + chr8_4:22710824-22710843 None:intergenic 30.0%
CATAAGCACAAATAGATTTG+GGG + chr8_4:22710825-22710844 None:intergenic 30.0%
ACGTGATAATACAAATTGGT+TGG - chr8_4:22711247-22711266 Msa1319490:intron 30.0%
TGATAATACAAATTGGTTGG+TGG - chr8_4:22711250-22711269 Msa1319490:intron 30.0%
!! TAATTTTGAAGAAGTAGACG+AGG - chr8_4:22711415-22711434 Msa1319490:intron 30.0%
! CCAAATTTGTGTCATTAGTT+GGG + chr8_4:22711442-22711461 None:intergenic 30.0%
! TCCAAATTTGTGTCATTAGT+TGG + chr8_4:22711443-22711462 None:intergenic 30.0%
! GTTCAACTATGATGATGAAA+TGG - chr8_4:22711471-22711490 Msa1319490:five_prime_UTR 30.0%
! TTTTTATCTCTACACCCAAA+AGG + chr8_4:22711547-22711566 None:intergenic 30.0%
ACGAATAAACCGTGAAATGA+CGG + chr8_4:22709033-22709052 None:intergenic 35.0%
ATAGACTGCATTTGTGTTTG+TGG - chr8_4:22709396-22709415 Msa1319490:three_prime_UTR 35.0%
CACAAAACTGAAACACCAAA+AGG + chr8_4:22709518-22709537 None:intergenic 35.0%
!! TGTTTCAGTTTTGTGCTGTT+AGG - chr8_4:22709523-22709542 Msa1319490:three_prime_UTR 35.0%
!! TTTGTGCTGTTAGGTTGTTT+TGG - chr8_4:22709532-22709551 Msa1319490:three_prime_UTR 35.0%
AAAGAAGCAATGGATCTGTT+TGG - chr8_4:22709672-22709691 Msa1319490:three_prime_UTR 35.0%
AGAAACCAAACATCTTCTCA+TGG - chr8_4:22709695-22709714 Msa1319490:three_prime_UTR 35.0%
ATTTGCCATGAGAAGATGTT+TGG + chr8_4:22709703-22709722 None:intergenic 35.0%
CATGGCAAATGTTTCATCAA+TGG - chr8_4:22709713-22709732 Msa1319490:three_prime_UTR 35.0%
TTCATCAATGGGAAATGGAA+CGG - chr8_4:22709725-22709744 Msa1319490:three_prime_UTR 35.0%
TGTTCTCAGTATTCAACACA+AGG + chr8_4:22709943-22709962 None:intergenic 35.0%
!! GCAATTGATTTAGCTCTGAT+TGG - chr8_4:22710114-22710133 Msa1319490:intron 35.0%
! TCTTTTATGTTTGCTTGTGC+GGG - chr8_4:22710247-22710266 Msa1319490:intron 35.0%
TGAACAGAGTAACGTCAATA+AGG - chr8_4:22710308-22710327 Msa1319490:intron 35.0%
! TTATCTAGTCAAAGCTCTAG+TGG + chr8_4:22710366-22710385 None:intergenic 35.0%
GGGTTGTACAATGATGAATT+TGG - chr8_4:22710404-22710423 Msa1319490:CDS 35.0%
ACCGTCTAAATCCAATAGAA+AGG - chr8_4:22710554-22710573 Msa1319490:intron 35.0%
ACTTGAAAAAAATGCAGCAG+AGG + chr8_4:22710740-22710759 None:intergenic 35.0%
TAAGCACAAATAGATTTGGG+GGG + chr8_4:22710823-22710842 None:intergenic 35.0%
! AGATACGACACTATGAGTTT+TGG - chr8_4:22710872-22710891 Msa1319490:intron 35.0%
! TTAGACCTGCCATTTCAAAA+AGG - chr8_4:22711113-22711132 Msa1319490:five_prime_UTR 35.0%
!!! ATCATCCTTTTTGAAATGGC+AGG + chr8_4:22711121-22711140 None:intergenic 35.0%
AGAAGAAGCAAAAAGAAAGC+AGG + chr8_4:22711159-22711178 None:intergenic 35.0%
! GCTTTCTTTTTGCTTCTTCT+TGG - chr8_4:22711159-22711178 Msa1319490:intron 35.0%
AAATGTTGTGAGCTACAATC+TGG - chr8_4:22711211-22711230 Msa1319490:intron 35.0%
! CTTGCATGTTCTCAACTTTT+TGG + chr8_4:22711340-22711359 None:intergenic 35.0%
! TGTTCAACTTTAGGAGTAAC+CGG + chr8_4:22711368-22711387 None:intergenic 35.0%
CCCAACTAATGACACAAATT+TGG - chr8_4:22711439-22711458 Msa1319490:five_prime_UTR 35.0%
GACACAAATTTGGAAGATGA+AGG - chr8_4:22711449-22711468 Msa1319490:five_prime_UTR 35.0%
GGGGATATTAGGGTTTCAAT+GGG + chr8_4:22708917-22708936 None:intergenic 40.0%
TGGGGATATTAGGGTTTCAA+TGG + chr8_4:22708918-22708937 None:intergenic 40.0%
GATGGAAGTTGGGGATATTA+GGG + chr8_4:22708927-22708946 None:intergenic 40.0%
AGATGGAAGTTGGGGATATT+AGG + chr8_4:22708928-22708947 None:intergenic 40.0%
GGAACAAAGCTGACAAATAG+AGG + chr8_4:22709111-22709130 None:intergenic 40.0%
!! TCAGCTTTGTTCCCCAATAT+AGG - chr8_4:22709118-22709137 Msa1319490:three_prime_UTR 40.0%
!! TTTACTTTGATGGGTTGCTC+AGG - chr8_4:22709358-22709377 Msa1319490:three_prime_UTR 40.0%
TAAAACACCAAAACCTGCCA+CGG + chr8_4:22709423-22709442 None:intergenic 40.0%
! CTTTTGATGCTTCTGCTGTT+TGG - chr8_4:22709492-22709511 Msa1319490:three_prime_UTR 40.0%
!! ACTTCCTCAGTTTGTTGTCA+AGG - chr8_4:22710076-22710095 Msa1319490:intron 40.0%
TGATTGGGAAAACAAGCTTG+AGG - chr8_4:22710130-22710149 Msa1319490:intron 40.0%
! CTTTTATGTTTGCTTGTGCG+GGG - chr8_4:22710248-22710267 Msa1319490:intron 40.0%
! TTTTATGTTTGCTTGTGCGG+GGG - chr8_4:22710249-22710268 Msa1319490:intron 40.0%
CTTGGGTGTTTAGTATGTGT+AGG - chr8_4:22710762-22710781 Msa1319490:intron 40.0%
AAGCACAAATAGATTTGGGG+GGG + chr8_4:22710822-22710841 None:intergenic 40.0%
AATAGGTGAACTGACTGCAA+TGG - chr8_4:22711010-22711029 Msa1319490:five_prime_UTR 40.0%
!! GTTGGTGGTTATCATGACAA+AGG - chr8_4:22711265-22711284 Msa1319490:intron 40.0%
TGAGAACATGCAAGTGTTAC+CGG - chr8_4:22711346-22711365 Msa1319490:intron 40.0%
AGAGAGTGAGATGGAAGTTG+GGG + chr8_4:22708936-22708955 None:intergenic 45.0%
GAGAGAGTGAGATGGAAGTT+GGG + chr8_4:22708937-22708956 None:intergenic 45.0%
AGTAGATCGAGAGAGTGAGA+TGG + chr8_4:22708945-22708964 None:intergenic 45.0%
AGATGAGAAGGAGATAGCGA+TGG + chr8_4:22708989-22709008 None:intergenic 45.0%
TTCCCTTCTCCGTCATTTCA+CGG - chr8_4:22709021-22709040 Msa1319490:three_prime_UTR 45.0%
! AACCGTGAAATGACGGAGAA+GGG + chr8_4:22709026-22709045 None:intergenic 45.0%
! AAACCGTGAAATGACGGAGA+AGG + chr8_4:22709027-22709046 None:intergenic 45.0%
GTTGCTCAGGACGTAATTTG+AGG - chr8_4:22709371-22709390 Msa1319490:three_prime_UTR 45.0%
!!! GGCAGGTTTTGGTGTTTTAG+TGG - chr8_4:22709424-22709443 Msa1319490:three_prime_UTR 45.0%
TTCAGACAGAAGAAGCAGAG+CGG - chr8_4:22709826-22709845 Msa1319490:three_prime_UTR 45.0%
! GAGGCTGACTCAGAAAAATG+AGG - chr8_4:22709881-22709900 Msa1319490:three_prime_UTR 45.0%
TCAGCCTTGACAACAAACTG+AGG + chr8_4:22710083-22710102 None:intergenic 45.0%
CGGTCGTGTTTAGCTAGATT+AGG + chr8_4:22710596-22710615 None:intergenic 45.0%
TAGCTAAACACGACCGTTGA+TGG - chr8_4:22710600-22710619 Msa1319490:CDS 45.0%
! GGGTTTAGAGCTTCCATCAA+CGG + chr8_4:22710616-22710635 None:intergenic 45.0%
!! TTGATGGAAGCTCTAAACCC+AGG - chr8_4:22710616-22710635 Msa1319490:CDS 45.0%
GGGTGTTTAGTATGTGTAGG+AGG - chr8_4:22710765-22710784 Msa1319490:intron 45.0%
AAATAGATTTGGGGGGGTCA+TGG + chr8_4:22710816-22710835 None:intergenic 45.0%
!! TAAATTATATAACAAAATTA+TGG + chr8_4:22711061-22711080 None:intergenic 5.0%
CGAGAGAGTGAGATGGAAGT+TGG + chr8_4:22708938-22708957 None:intergenic 50.0%
CTCTCGATCTACTCCACTGA+AGG - chr8_4:22708952-22708971 Msa1319490:three_prime_UTR 50.0%
CGATGTGGTCAGAGATGAGA+AGG + chr8_4:22709001-22709020 None:intergenic 50.0%
GCATTTGTGTTTGTGGACCG+TGG - chr8_4:22709403-22709422 Msa1319490:three_prime_UTR 50.0%
CTGCTGTTATAGCTCTCGAC+TGG - chr8_4:22709469-22709488 Msa1319490:three_prime_UTR 50.0%
!! GCTTCTGCTGTTTGGCCTTT+TGG - chr8_4:22709500-22709519 Msa1319490:three_prime_UTR 50.0%
GCAGCTGAATGAGCTAGCAT+TGG - chr8_4:22709636-22709655 Msa1319490:three_prime_UTR 50.0%
GGAGACAAGAGAGCCTTCAG+TGG + chr8_4:22708968-22708987 None:intergenic 55.0%
TGACGGAGAAGGGAACGATG+TGG + chr8_4:22709016-22709035 None:intergenic 55.0%
TTGTGTTTGTGGACCGTGGC+AGG - chr8_4:22709407-22709426 Msa1319490:three_prime_UTR 55.0%
! TTGTGGACCGTGGCAGGTTT+TGG - chr8_4:22709413-22709432 Msa1319490:three_prime_UTR 55.0%
CAGAAGAAGCAGAGCGGTGC+TGG - chr8_4:22709832-22709851 Msa1319490:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 22708870 22711649 22708870 ID=Msa1319490;Name=Msa1319490
chr8_4 mRNA 22708870 22711649 22708870 ID=Msa1319490-mRNA-1;Parent=Msa1319490;Name=Msa1319490-mRNA-1;_AED=0.07;_eAED=0.07;_QI=430|0.8|0.83|1|0.4|0.16|6|1130|79
chr8_4 exon 22708870 22709969 22708870 ID=Msa1319490-mRNA-1:exon:8052;Parent=Msa1319490-mRNA-1
chr8_4 exon 22710368 22710456 22710368 ID=Msa1319490-mRNA-1:exon:8051;Parent=Msa1319490-mRNA-1
chr8_4 exon 22710574 22710688 22710574 ID=Msa1319490-mRNA-1:exon:8050;Parent=Msa1319490-mRNA-1
chr8_4 exon 22710773 22710876 22710773 ID=Msa1319490-mRNA-1:exon:8049;Parent=Msa1319490-mRNA-1
chr8_4 exon 22710975 22711139 22710975 ID=Msa1319490-mRNA-1:exon:8048;Parent=Msa1319490-mRNA-1
chr8_4 exon 22711423 22711649 22711423 ID=Msa1319490-mRNA-1:exon:8047;Parent=Msa1319490-mRNA-1
chr8_4 five_prime_UTR 22711423 22711649 22711423 ID=Msa1319490-mRNA-1:five_prime_utr;Parent=Msa1319490-mRNA-1
chr8_4 five_prime_UTR 22710975 22711139 22710975 ID=Msa1319490-mRNA-1:five_prime_utr;Parent=Msa1319490-mRNA-1
chr8_4 five_prime_UTR 22710839 22710876 22710839 ID=Msa1319490-mRNA-1:five_prime_utr;Parent=Msa1319490-mRNA-1
chr8_4 CDS 22710773 22710838 22710773 ID=Msa1319490-mRNA-1:cds;Parent=Msa1319490-mRNA-1
chr8_4 CDS 22710574 22710688 22710574 ID=Msa1319490-mRNA-1:cds;Parent=Msa1319490-mRNA-1
chr8_4 CDS 22710398 22710456 22710398 ID=Msa1319490-mRNA-1:cds;Parent=Msa1319490-mRNA-1
chr8_4 three_prime_UTR 22710368 22710397 22710368 ID=Msa1319490-mRNA-1:three_prime_utr;Parent=Msa1319490-mRNA-1
chr8_4 three_prime_UTR 22708870 22709969 22708870 ID=Msa1319490-mRNA-1:three_prime_utr;Parent=Msa1319490-mRNA-1
Gene Sequence

>Msa1319490

ATGGATCTGTTTGGAGAAACCAAACATCTTCTCATGGCAAATGTTTCATCAATGGGAAATGGAACGACAGAAGAAGCAGAGCGGTGCTGGTTTGCTTTTATTTTATACTCTATCAAGAGGCTGACTCAGAAAAATGAGGAGAGTGAAAAAGATGAGATTGAGAATACTGGTCTTACCTTGTATTTTTTCAAAGAACTTCCTCAGTTTGTTGTCAAGGCTGATCCAATTTTAAGCAATTGA
Protein sequence

>Msa1319490

MDLFGETKHLLMANVSSMGNGTTEEAERCWFAFILYSIKRLTQKNEESEKDEIENTGLTLYFFKELPQFVVKADPILSN*