Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212030 | AFK43929.1 | 98.571 | 70 | 1 | 0 | 41 | 110 | 1 | 70 | 2.45e-41 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212030 | sp|Q9ASY8|TOM71_ARATH | 67.308 | 52 | 17 | 0 | 59 | 110 | 24 | 75 | 1.14e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212030 | I3T329 | 98.571 | 70 | 1 | 0 | 41 | 110 | 1 | 70 | 1.17e-41 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0212030 | Msa1351920 | -0.809986 | 1.442820e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212030 | MtrunA17_Chr2g0311211 | 98.571 | 70 | 1 | 0 | 41 | 110 | 1 | 70 | 2.26e-45 | 141 |
| Msa0212030 | MtrunA17_Chr3g0103621 | 90.000 | 70 | 7 | 0 | 41 | 110 | 1 | 70 | 1.05e-40 | 129 |
| Msa0212030 | MtrunA17_Chr3g0100491 | 85.507 | 69 | 10 | 0 | 41 | 109 | 1 | 69 | 1.20e-38 | 124 |
| Msa0212030 | MtrunA17_Chr3g0103591 | 80.000 | 70 | 14 | 0 | 41 | 110 | 1 | 70 | 2.20e-34 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0212030 | AT5G41685.1 | 67.308 | 52 | 17 | 0 | 59 | 110 | 24 | 75 | 1.16e-22 | 84.3 |
| Msa0212030 | AT1G64220.1 | 72.093 | 43 | 12 | 0 | 68 | 110 | 35 | 77 | 6.09e-18 | 72.4 |
Find 28 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGAGAAATTTGTGGTTT+TGG | 0.210299 | 2_1:+53660363 | None:intergenic |
| CCAATGATGTTAAAGTTTCT+TGG | 0.256698 | 2_1:-53660139 | Msa0212030:three_prime_UTR |
| CCAAGAAACTTTAACATCAT+TGG | 0.283864 | 2_1:+53660139 | None:intergenic |
| ACAAAGGTTATTGCTCACTA+TGG | 0.348329 | 2_1:-53660426 | Msa0212030:CDS |
| CAACACAGACACTTTGTTCC+TGG | 0.376546 | 2_1:-53660737 | Msa0212030:CDS |
| ATTCCTTTGATTATCATCAT+TGG | 0.388372 | 2_1:-53660399 | Msa0212030:CDS |
| AGAAGACGAGAGAGAGAGCC+AGG | 0.391716 | 2_1:+53660719 | None:intergenic |
| CTGAGAAGCTGAGAAATTTG+TGG | 0.449278 | 2_1:+53660357 | None:intergenic |
| ATGTTTATGCTTGGTTGAGC+AGG | 0.453629 | 2_1:-53660176 | Msa0212030:three_prime_UTR |
| TACTTGATGATGTTTATGCT+TGG | 0.483997 | 2_1:-53660185 | Msa0212030:three_prime_UTR |
| GAAAAGAAGAATGGCGCCTA+GGG | 0.517130 | 2_1:-53660547 | Msa0212030:CDS |
| CTGTGAAGGAATGGACAACA+TGG | 0.524007 | 2_1:-53660461 | Msa0212030:CDS |
| TTGTACTTCTCTCTATCGAA+AGG | 0.526051 | 2_1:-53660767 | Msa0212030:CDS |
| TGATCGATTCTGTGAAGGAA+TGG | 0.539433 | 2_1:-53660470 | Msa0212030:CDS |
| TTTGCTTTCAGAGAAACCCT+AGG | 0.540311 | 2_1:+53660531 | None:intergenic |
| AATGTGCAACATCGATTACA+CGG | 0.540372 | 2_1:+53660332 | None:intergenic |
| AGGGTTTCTCTGAAAGCAAA+GGG | 0.547703 | 2_1:-53660528 | Msa0212030:CDS |
| GAGCGAAGAGAAAAGAAGAA+TGG | 0.558171 | 2_1:-53660556 | Msa0212030:CDS |
| AGAAAAGAAGAATGGCGCCT+AGG | 0.558230 | 2_1:-53660548 | Msa0212030:CDS |
| TAGGGTTTCTCTGAAAGCAA+AGG | 0.590299 | 2_1:-53660529 | Msa0212030:CDS |
| ATACCAATGATGATAATCAA+AGG | 0.596226 | 2_1:+53660396 | None:intergenic |
| ATCAGTGATCGATTCTGTGA+AGG | 0.598645 | 2_1:-53660475 | Msa0212030:CDS |
| ATGTGCAACATCGATTACAC+GGG | 0.623342 | 2_1:+53660333 | None:intergenic |
| TGTGAAGGAATGGACAACAT+GGG | 0.649737 | 2_1:-53660460 | Msa0212030:CDS |
| GGAATATTATGGAATCAATG+TGG | 0.678165 | 2_1:-53660291 | Msa0212030:three_prime_UTR |
| ATGGGGTATGAAGAAAACAA+AGG | 0.679010 | 2_1:-53660442 | Msa0212030:CDS |
| GTGAAGGAATGGACAACATG+GGG | 0.691902 | 2_1:-53660459 | Msa0212030:CDS |
| TGTGCAACATCGATTACACG+GGG | 0.748285 | 2_1:+53660334 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAGAAATTAATGAAAATT+TGG | + | chr2_1:53660251-53660270 | None:intergenic | 15.0% |
| !! | CAAAGATAACAAAAAAAAAT+TGG | + | chr2_1:53660298-53660317 | None:intergenic | 15.0% |
| !!! | TTCTGTAGTTTTTTACTTTT+TGG | - | chr2_1:53660616-53660635 | Msa0212030:intron | 20.0% |
| !!! | TCTGTAGTTTTTTACTTTTT+GGG | - | chr2_1:53660617-53660636 | Msa0212030:intron | 20.0% |
| !! | CTTGGTTAAAAAAATGAATT+AGG | - | chr2_1:53660760-53660779 | Msa0212030:CDS | 20.0% |
| ! | ATTCCTTTGATTATCATCAT+TGG | - | chr2_1:53660482-53660501 | Msa0212030:CDS | 25.0% |
| ! | ATACCAATGATGATAATCAA+AGG | + | chr2_1:53660488-53660507 | None:intergenic | 25.0% |
| !! | ATTTTCACTTGCTTTTCTGA+TGG | - | chr2_1:53660569-53660588 | Msa0212030:intron | 30.0% |
| ! | GCTTTTCTGATGGAATATTA+TGG | - | chr2_1:53660579-53660598 | Msa0212030:intron | 30.0% |
| GGAATATTATGGAATCAATG+TGG | - | chr2_1:53660590-53660609 | Msa0212030:intron | 30.0% | |
| !! | TACTTGATGATGTTTATGCT+TGG | - | chr2_1:53660696-53660715 | Msa0212030:CDS | 30.0% |
| CCAAGAAACTTTAACATCAT+TGG | + | chr2_1:53660745-53660764 | None:intergenic | 30.0% | |
| CCAATGATGTTAAAGTTTCT+TGG | - | chr2_1:53660742-53660761 | Msa0212030:CDS | 30.0% | |
| TTGTACTTCTCTCTATCGAA+AGG | - | chr2_1:53660114-53660133 | Msa0212030:three_prime_UTR | 35.0% | |
| ATGGGGTATGAAGAAAACAA+AGG | - | chr2_1:53660439-53660458 | Msa0212030:CDS | 35.0% | |
| ACAAAGGTTATTGCTCACTA+TGG | - | chr2_1:53660455-53660474 | Msa0212030:CDS | 35.0% | |
| ! | AGCTGAGAAATTTGTGGTTT+TGG | + | chr2_1:53660521-53660540 | None:intergenic | 35.0% |
| ! | AATGTGCAACATCGATTACA+CGG | + | chr2_1:53660552-53660571 | None:intergenic | 35.0% |
| GAAAAGAGAGTCGAATCGAA+AGG | + | chr2_1:53660224-53660243 | None:intergenic | 40.0% | |
| GAGCGAAGAGAAAAGAAGAA+TGG | - | chr2_1:53660325-53660344 | Msa0212030:three_prime_UTR | 40.0% | |
| TTTGCTTTCAGAGAAACCCT+AGG | + | chr2_1:53660353-53660372 | None:intergenic | 40.0% | |
| ! | TAGGGTTTCTCTGAAAGCAA+AGG | - | chr2_1:53660352-53660371 | Msa0212030:CDS | 40.0% |
| ! | AGGGTTTCTCTGAAAGCAAA+GGG | - | chr2_1:53660353-53660372 | Msa0212030:CDS | 40.0% |
| ! | ATCAGTGATCGATTCTGTGA+AGG | - | chr2_1:53660406-53660425 | Msa0212030:CDS | 40.0% |
| ! | TGATCGATTCTGTGAAGGAA+TGG | - | chr2_1:53660411-53660430 | Msa0212030:CDS | 40.0% |
| TGTGAAGGAATGGACAACAT+GGG | - | chr2_1:53660421-53660440 | Msa0212030:CDS | 40.0% | |
| CTGAGAAGCTGAGAAATTTG+TGG | + | chr2_1:53660527-53660546 | None:intergenic | 40.0% | |
| ! | ATGTGCAACATCGATTACAC+GGG | + | chr2_1:53660551-53660570 | None:intergenic | 40.0% |
| ATGTTTATGCTTGGTTGAGC+AGG | - | chr2_1:53660705-53660724 | Msa0212030:CDS | 40.0% | |
| CAACACAGACACTTTGTTCC+TGG | - | chr2_1:53660144-53660163 | Msa0212030:three_prime_UTR | 45.0% | |
| AGAAAAGAAGAATGGCGCCT+AGG | - | chr2_1:53660333-53660352 | Msa0212030:exon | 45.0% | |
| GAAAAGAAGAATGGCGCCTA+GGG | - | chr2_1:53660334-53660353 | Msa0212030:exon | 45.0% | |
| CTGTGAAGGAATGGACAACA+TGG | - | chr2_1:53660420-53660439 | Msa0212030:CDS | 45.0% | |
| GTGAAGGAATGGACAACATG+GGG | - | chr2_1:53660422-53660441 | Msa0212030:CDS | 45.0% | |
| ! | TGTGCAACATCGATTACACG+GGG | + | chr2_1:53660550-53660569 | None:intergenic | 45.0% |
| AGAAGACGAGAGAGAGAGCC+AGG | + | chr2_1:53660165-53660184 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 53660114 | 53660789 | 53660114 | ID=Msa0212030;Name=Msa0212030 |
| chr2_1 | mRNA | 53660114 | 53660789 | 53660114 | ID=Msa0212030-mRNA-1;Parent=Msa0212030;Name=Msa0212030-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0|0.5|1|0|0|2|233|110 |
| chr2_1 | exon | 53660114 | 53660577 | 53660114 | ID=Msa0212030-mRNA-1:exon:14936;Parent=Msa0212030-mRNA-1 |
| chr2_1 | exon | 53660688 | 53660789 | 53660688 | ID=Msa0212030-mRNA-1:exon:14935;Parent=Msa0212030-mRNA-1 |
| chr2_1 | CDS | 53660688 | 53660789 | 53660688 | ID=Msa0212030-mRNA-1:cds;Parent=Msa0212030-mRNA-1 |
| chr2_1 | CDS | 53660347 | 53660577 | 53660347 | ID=Msa0212030-mRNA-1:cds;Parent=Msa0212030-mRNA-1 |
| chr2_1 | three_prime_UTR | 53660114 | 53660346 | 53660114 | ID=Msa0212030-mRNA-1:three_prime_utr;Parent=Msa0212030-mRNA-1 |
| Gene Sequence |
| Protein sequence |
