AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0215550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215550 MtrunA17_Chr2g0316841 97.205 322 9 0 1 322 1 322 0.0 646
Msa0215550 MtrunA17_Chr2g0319971 47.059 102 42 5 225 319 134 230 3.49e-18 82.8
Msa0215550 MtrunA17_Chr2g0284311 39.053 169 83 5 167 320 66 229 4.20e-18 82.0
Msa0215550 MtrunA17_Chr8g0379321 41.322 121 52 5 216 320 123 240 9.39e-17 78.2
Msa0215550 MtrunA17_Chr4g0041451 40.367 109 47 3 229 319 128 236 5.18e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0215550 AT3G15115.1 29.429 350 171 15 18 319 12 333 1.51e-19 88.2

Find 66 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCTTATTTCCCCAATT+TGG 0.135591 2_1:+60839996 None:intergenic
TCTTCTTCTTTCACTCTAAT+TGG 0.176697 2_1:+60841290 None:intergenic
CAACTCCTCCTGTTCCAAAT+TGG 0.187842 2_1:-60840010 Msa0215550:CDS
CTTAAACAAAGAGGGTATTT+TGG 0.192411 2_1:-60840267 Msa0215550:CDS
TGGAGGCAAGTTGTCATTAT+TGG 0.201109 2_1:+60841028 None:intergenic
GTATGTATTCTGAATTTATA+TGG 0.253315 2_1:+60841614 None:intergenic
GGTGCTCTTTGCAGCTTCTT+AGG 0.254314 2_1:+60841323 None:intergenic
GAATGACACCTTAACATCTT+TGG 0.264564 2_1:+60841434 None:intergenic
CAAGAAACTGATTCAAGAAT+TGG 0.268407 2_1:-60841080 Msa0215550:CDS
AACTCCTCCTGTTCCAAATT+GGG 0.290556 2_1:-60840009 Msa0215550:CDS
GGAGGCAAGTTGTCATTATT+GGG 0.324183 2_1:+60841029 None:intergenic
CAAGAGCTATTATTATCATT+TGG 0.336403 2_1:+60841545 None:intergenic
CGCATCTATTATCTATAGTT+AGG 0.337594 2_1:+60839803 None:intergenic
ACTCTAATTGGTGGCATAGA+TGG 0.345119 2_1:+60841302 None:intergenic
CCATCTTTGCCTCCAACAAT+AGG 0.347224 2_1:-60841119 Msa0215550:CDS
AGCTTAGCTAACATAATTCC+TGG 0.370469 2_1:-60840122 Msa0215550:CDS
GCCTCCAAGGTTAACCTCTA+AGG 0.388438 2_1:-60841012 Msa0215550:intron
TCCTCTCTCCCTATTGTTGG+AGG 0.400398 2_1:+60841110 None:intergenic
TTAGTCATCCACAAAGTTGA+TGG 0.405237 2_1:+60839774 None:intergenic
TCCTAAATCAGAAAACAATA+AGG 0.414045 2_1:-60840229 Msa0215550:CDS
CGAAGTATCCATCAACTTTG+TGG 0.415896 2_1:-60839782 Msa0215550:three_prime_UTR
TTATTTCCCCAATTTGGAAC+AGG 0.427610 2_1:+60840002 None:intergenic
ATATAAAACCAAAGATGTTA+AGG 0.433545 2_1:-60841442 Msa0215550:CDS
GGAATTATGTTAGCTAAGCT+TGG 0.440726 2_1:+60840125 None:intergenic
AGAATACATACATACATGTA+AGG 0.443988 2_1:-60841602 Msa0215550:five_prime_UTR
TCTTCTTTCACTCTAATTGG+TGG 0.448745 2_1:+60841293 None:intergenic
CCTATTGTTGGAGGCAAAGA+TGG 0.449300 2_1:+60841119 None:intergenic
CCCACTAAAGGTGGCCAACT+AGG 0.457150 2_1:+60839738 None:intergenic
CTTGAGTATGAAGAAGTTCA+AGG 0.460785 2_1:-60840191 Msa0215550:CDS
ATAGAAGTGATAGCAATAGA+AGG 0.463972 2_1:-60841163 Msa0215550:CDS
TATGTATTCTGAATTTATAT+GGG 0.471012 2_1:+60841615 None:intergenic
ATTAGTCTTGTTGAAGGGTA+AGG 0.475567 2_1:+60841407 None:intergenic
GTTCAAGGGTTCAAGGACTT+AGG 0.476645 2_1:-60840176 Msa0215550:CDS
CATCTTTGCCTCCAACAATA+GGG 0.480386 2_1:-60841118 Msa0215550:CDS
ACCTTAGAGGTTAACCTTGG+AGG 0.482914 2_1:+60841011 None:intergenic
GAGCTGATTAGTCTTGTTGA+AGG 0.486253 2_1:+60841401 None:intergenic
ATCGGATTCTGCTATGCATA+AGG 0.492617 2_1:-60841228 Msa0215550:CDS
AGTACCTTAGAGGTTAACCT+TGG 0.525551 2_1:+60841008 None:intergenic
AACCATGCCTCTGATAAGTA+AGG 0.533103 2_1:+60840047 None:intergenic
TCATTATTGGGAGGTGTCGA+TGG 0.539813 2_1:+60841041 None:intergenic
TGAAGAAGTTCAAGGGTTCA+AGG 0.542575 2_1:-60840183 Msa0215550:CDS
ATAATGACAACTTGCCTCCA+AGG 0.544515 2_1:-60841025 Msa0215550:CDS
ATCTCCGTCGAAAACCTAGT+TGG 0.548097 2_1:-60839752 Msa0215550:three_prime_UTR
TTACTTATCAGAGGCATGGT+TGG 0.560586 2_1:-60840045 Msa0215550:CDS
CATCATTGTGCAAAGATGAA+AGG 0.561895 2_1:-60841194 Msa0215550:CDS
ACTCCTCCTGTTCCAAATTG+GGG 0.566945 2_1:-60840008 Msa0215550:CDS
TGAATCTACTTGAAGAGTGT+TGG 0.568822 2_1:-60841484 Msa0215550:CDS
CTTCATACTCAAGATCACTT+AGG 0.571003 2_1:+60840201 None:intergenic
CAGCATTCCAAGAAGTAGAC+CGG 0.572646 2_1:-60840312 Msa0215550:CDS
GACCTTACTTATCAGAGGCA+TGG 0.573969 2_1:-60840049 Msa0215550:CDS
ATTGAAAGCTTAAACAAAGA+GGG 0.576048 2_1:-60840275 Msa0215550:CDS
AGCTGATTAGTCTTGTTGAA+GGG 0.577448 2_1:+60841402 None:intergenic
TTTCCCCAATTTGGAACAGG+AGG 0.577483 2_1:+60840005 None:intergenic
GATTCTGCTATGCATAAGGC+AGG 0.577729 2_1:-60841224 Msa0215550:CDS
AATTGAAAGCTTAAACAAAG+AGG 0.586217 2_1:-60840276 Msa0215550:CDS
GCCTCCAACAATAGGGAGAG+AGG 0.599344 2_1:-60841111 Msa0215550:CDS
TTGAGTATGAAGAAGTTCAA+GGG 0.610506 2_1:-60840190 Msa0215550:CDS
GAGGAAGATAAAGCAGCAAG+AGG 0.618815 2_1:-60840071 Msa0215550:CDS
GAATACATACATACATGTAA+GGG 0.624199 2_1:-60841601 Msa0215550:five_prime_UTR
CAAATTGGTGCATCAAACAT+CGG 0.624498 2_1:-60841246 Msa0215550:CDS
GGCAAGTTGTCATTATTGGG+AGG 0.626871 2_1:+60841032 None:intergenic
AGGATGAACAAAATCACCAT+CGG 0.638726 2_1:+60841343 None:intergenic
GTTAGAAAAGTTGAAACACA+TGG 0.639189 2_1:-60841570 Msa0215550:exon
AAGAGGACCTTACTTATCAG+AGG 0.645280 2_1:-60840054 Msa0215550:CDS
TGAACAAAATCACCATCGGA+TGG 0.647374 2_1:+60841347 None:intergenic
TCAGCAAGTAAACCATCCGA+TGG 0.664328 2_1:-60841359 Msa0215550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAAATACTAAAATTATT+TGG + chr2_1:60840720-60840739 None:intergenic 10.0%
!! ACTTAAATACTAAAATTATT+TGG + chr2_1:60840720-60840739 None:intergenic 10.0%
!! TATGTATTCTGAATTTATAT+GGG + chr2_1:60839786-60839805 None:intergenic 15.0%
!! ATAATGATTTAAAAAAAGGT+AGG + chr2_1:60840616-60840635 None:intergenic 15.0%
!! TGACATAATGATTTAAAAAA+AGG + chr2_1:60840620-60840639 None:intergenic 15.0%
!! TATGTATTCTGAATTTATAT+GGG + chr2_1:60839786-60839805 None:intergenic 15.0%
!! ATAATGATTTAAAAAAAGGT+AGG + chr2_1:60840616-60840635 None:intergenic 15.0%
!! TGACATAATGATTTAAAAAA+AGG + chr2_1:60840620-60840639 None:intergenic 15.0%
!! GTATGTATTCTGAATTTATA+TGG + chr2_1:60839787-60839806 None:intergenic 20.0%
!! ATATAAAACCAAAGATGTTA+AGG - chr2_1:60839956-60839975 Msa0215550:CDS 20.0%
!! GAAGAAAAAGATGATAAAAA+AGG - chr2_1:60840126-60840145 Msa0215550:CDS 20.0%
!! TAATATCAATCATAAACTCT+TGG + chr2_1:60840646-60840665 None:intergenic 20.0%
!! AACACCAATTAATACAAATA+TGG - chr2_1:60840686-60840705 Msa0215550:intron 20.0%
!! TTAGCCATATTTGTATTAAT+TGG + chr2_1:60840693-60840712 None:intergenic 20.0%
!! TTAATACAAATATGGCTAAA+AGG - chr2_1:60840694-60840713 Msa0215550:intron 20.0%
!! ATGATCAATCAAAATATGAT+TGG + chr2_1:60840913-60840932 None:intergenic 20.0%
!! GTACATATATTCAAGAATAT+AGG + chr2_1:60840989-60841008 None:intergenic 20.0%
!!! TGAAAAAACATATCAAGTTT+TGG - chr2_1:60841436-60841455 Msa0215550:CDS 20.0%
!!! GAAAAAACATATCAAGTTTT+GGG - chr2_1:60841437-60841456 Msa0215550:CDS 20.0%
!!! TTATTCTTTTGATTGTTCTT+AGG + chr2_1:60841543-60841562 None:intergenic 20.0%
!! GTATGTATTCTGAATTTATA+TGG + chr2_1:60839787-60839806 None:intergenic 20.0%
!! ATATAAAACCAAAGATGTTA+AGG - chr2_1:60839956-60839975 Msa0215550:CDS 20.0%
!! GAAGAAAAAGATGATAAAAA+AGG - chr2_1:60840126-60840145 Msa0215550:CDS 20.0%
!! TAATATCAATCATAAACTCT+TGG + chr2_1:60840646-60840665 None:intergenic 20.0%
!! AACACCAATTAATACAAATA+TGG - chr2_1:60840686-60840705 Msa0215550:intron 20.0%
!! TTAGCCATATTTGTATTAAT+TGG + chr2_1:60840693-60840712 None:intergenic 20.0%
!! TTAATACAAATATGGCTAAA+AGG - chr2_1:60840694-60840713 Msa0215550:intron 20.0%
!! ATGATCAATCAAAATATGAT+TGG + chr2_1:60840913-60840932 None:intergenic 20.0%
!! GTACATATATTCAAGAATAT+AGG + chr2_1:60840989-60841008 None:intergenic 20.0%
!!! TGAAAAAACATATCAAGTTT+TGG - chr2_1:60841436-60841455 Msa0215550:CDS 20.0%
!!! GAAAAAACATATCAAGTTTT+GGG - chr2_1:60841437-60841456 Msa0215550:CDS 20.0%
!!! TTATTCTTTTGATTGTTCTT+AGG + chr2_1:60841543-60841562 None:intergenic 20.0%
! AGAATACATACATACATGTA+AGG - chr2_1:60839796-60839815 Msa0215550:three_prime_UTR 25.0%
! GAATACATACATACATGTAA+GGG - chr2_1:60839797-60839816 Msa0215550:three_prime_UTR 25.0%
! CAAGAGCTATTATTATCATT+TGG + chr2_1:60839856-60839875 None:intergenic 25.0%
!! TTTTTGCAAAGATGAAAAAG+TGG - chr2_1:60839885-60839904 Msa0215550:three_prime_UTR 25.0%
! TGATAAAAAAGGAAGCAAAT+TGG - chr2_1:60840137-60840156 Msa0215550:CDS 25.0%
!! TGATTTTTATAGTACCTTAG+AGG + chr2_1:60840403-60840422 None:intergenic 25.0%
! CGAAGAATTGTGATTTAATA+TGG - chr2_1:60840523-60840542 Msa0215550:intron 25.0%
!!! TATTAATTGGTGTTACTTCT+TGG + chr2_1:60840680-60840699 None:intergenic 25.0%
! ATAGCATCAGAAATAGAAAA+GGG - chr2_1:60840746-60840765 Msa0215550:intron 25.0%
! ATATTTAAACATAGCATGAC+AGG + chr2_1:60840821-60840840 None:intergenic 25.0%
! AATTGAAAGCTTAAACAAAG+AGG - chr2_1:60841122-60841141 Msa0215550:CDS 25.0%
! ATTGAAAGCTTAAACAAAGA+GGG - chr2_1:60841123-60841142 Msa0215550:CDS 25.0%
! TCCTAAATCAGAAAACAATA+AGG - chr2_1:60841169-60841188 Msa0215550:CDS 25.0%
!! TCCTTATTGTTTTCTGATTT+AGG + chr2_1:60841173-60841192 None:intergenic 25.0%
! AGAATACATACATACATGTA+AGG - chr2_1:60839796-60839815 Msa0215550:three_prime_UTR 25.0%
! GAATACATACATACATGTAA+GGG - chr2_1:60839797-60839816 Msa0215550:three_prime_UTR 25.0%
! CAAGAGCTATTATTATCATT+TGG + chr2_1:60839856-60839875 None:intergenic 25.0%
!! TTTTTGCAAAGATGAAAAAG+TGG - chr2_1:60839885-60839904 Msa0215550:three_prime_UTR 25.0%
! TGATAAAAAAGGAAGCAAAT+TGG - chr2_1:60840137-60840156 Msa0215550:CDS 25.0%
!! TGATTTTTATAGTACCTTAG+AGG + chr2_1:60840403-60840422 None:intergenic 25.0%
! CGAAGAATTGTGATTTAATA+TGG - chr2_1:60840523-60840542 Msa0215550:intron 25.0%
!!! TATTAATTGGTGTTACTTCT+TGG + chr2_1:60840680-60840699 None:intergenic 25.0%
! ATAGCATCAGAAATAGAAAA+GGG - chr2_1:60840746-60840765 Msa0215550:intron 25.0%
! ATATTTAAACATAGCATGAC+AGG + chr2_1:60840821-60840840 None:intergenic 25.0%
! AATTGAAAGCTTAAACAAAG+AGG - chr2_1:60841122-60841141 Msa0215550:CDS 25.0%
! ATTGAAAGCTTAAACAAAGA+GGG - chr2_1:60841123-60841142 Msa0215550:CDS 25.0%
! TCCTAAATCAGAAAACAATA+AGG - chr2_1:60841169-60841188 Msa0215550:CDS 25.0%
!! TCCTTATTGTTTTCTGATTT+AGG + chr2_1:60841173-60841192 None:intergenic 25.0%
GTTAGAAAAGTTGAAACACA+TGG - chr2_1:60839828-60839847 Msa0215550:three_prime_UTR 30.0%
! AATCAGCTCAGAATTTTTGA+AGG - chr2_1:60840011-60840030 Msa0215550:CDS 30.0%
TCTTCTTCTTTCACTCTAAT+TGG + chr2_1:60840111-60840130 None:intergenic 30.0%
ATAGAAGTGATAGCAATAGA+AGG - chr2_1:60840235-60840254 Msa0215550:CDS 30.0%
! CAAGAAACTGATTCAAGAAT+TGG - chr2_1:60840318-60840337 Msa0215550:CDS 30.0%
ATTTAATATGGCAAGTAGTC+TGG - chr2_1:60840535-60840554 Msa0215550:intron 30.0%
TATGATTGATATTATGTGCC+TGG - chr2_1:60840652-60840671 Msa0215550:intron 30.0%
CATAGCATCAGAAATAGAAA+AGG - chr2_1:60840745-60840764 Msa0215550:intron 30.0%
ACAATAATACGTGAAAGGAA+GGG + chr2_1:60840950-60840969 None:intergenic 30.0%
AACAATAATACGTGAAAGGA+AGG + chr2_1:60840951-60840970 None:intergenic 30.0%
ATGCAACAATAATACGTGAA+AGG + chr2_1:60840955-60840974 None:intergenic 30.0%
!!! TCAATTTCGATGTTTTTAGC+CGG + chr2_1:60841108-60841127 None:intergenic 30.0%
!!! CTTAAACAAAGAGGGTATTT+TGG - chr2_1:60841131-60841150 Msa0215550:CDS 30.0%
TTGAGTATGAAGAAGTTCAA+GGG - chr2_1:60841208-60841227 Msa0215550:CDS 30.0%
GAAAAATAGAGATGAAAGTG+AGG - chr2_1:60841308-60841327 Msa0215550:CDS 30.0%
CGCATCTATTATCTATAGTT+AGG + chr2_1:60841598-60841617 None:intergenic 30.0%
! ATCTTGTGACACTGAATTTT+TGG + chr2_1:60839753-60839772 None:intergenic 30.0%
GTTAGAAAAGTTGAAACACA+TGG - chr2_1:60839828-60839847 Msa0215550:three_prime_UTR 30.0%
! AATCAGCTCAGAATTTTTGA+AGG - chr2_1:60840011-60840030 Msa0215550:CDS 30.0%
TCTTCTTCTTTCACTCTAAT+TGG + chr2_1:60840111-60840130 None:intergenic 30.0%
ATAGAAGTGATAGCAATAGA+AGG - chr2_1:60840235-60840254 Msa0215550:CDS 30.0%
! CAAGAAACTGATTCAAGAAT+TGG - chr2_1:60840318-60840337 Msa0215550:CDS 30.0%
ATTTAATATGGCAAGTAGTC+TGG - chr2_1:60840535-60840554 Msa0215550:intron 30.0%
TATGATTGATATTATGTGCC+TGG - chr2_1:60840652-60840671 Msa0215550:intron 30.0%
CATAGCATCAGAAATAGAAA+AGG - chr2_1:60840745-60840764 Msa0215550:intron 30.0%
ACAATAATACGTGAAAGGAA+GGG + chr2_1:60840950-60840969 None:intergenic 30.0%
AACAATAATACGTGAAAGGA+AGG + chr2_1:60840951-60840970 None:intergenic 30.0%
ATGCAACAATAATACGTGAA+AGG + chr2_1:60840955-60840974 None:intergenic 30.0%
!!! TCAATTTCGATGTTTTTAGC+CGG + chr2_1:60841108-60841127 None:intergenic 30.0%
!!! CTTAAACAAAGAGGGTATTT+TGG - chr2_1:60841131-60841150 Msa0215550:CDS 30.0%
TTGAGTATGAAGAAGTTCAA+GGG - chr2_1:60841208-60841227 Msa0215550:CDS 30.0%
GAAAAATAGAGATGAAAGTG+AGG - chr2_1:60841308-60841327 Msa0215550:CDS 30.0%
CGCATCTATTATCTATAGTT+AGG + chr2_1:60841598-60841617 None:intergenic 30.0%
! TGAATCTACTTGAAGAGTGT+TGG - chr2_1:60839914-60839933 Msa0215550:exon 35.0%
GAATGACACCTTAACATCTT+TGG + chr2_1:60839967-60839986 None:intergenic 35.0%
!! ATTAGTCTTGTTGAAGGGTA+AGG + chr2_1:60839994-60840013 None:intergenic 35.0%
!! AGCTGATTAGTCTTGTTGAA+GGG + chr2_1:60839999-60840018 None:intergenic 35.0%
AGGATGAACAAAATCACCAT+CGG + chr2_1:60840058-60840077 None:intergenic 35.0%
TCTTCTTTCACTCTAATTGG+TGG + chr2_1:60840108-60840127 None:intergenic 35.0%
CAAATTGGTGCATCAAACAT+CGG - chr2_1:60840152-60840171 Msa0215550:CDS 35.0%
CATCATTGTGCAAAGATGAA+AGG - chr2_1:60840204-60840223 Msa0215550:CDS 35.0%
TACCAAAAAGAGAAGAATGC+AGG - chr2_1:60840871-60840890 Msa0215550:intron 35.0%
! TTCCTGCATTCTTCTCTTTT+TGG + chr2_1:60840876-60840895 None:intergenic 35.0%
AGAGAAGAATGCAGGAATAT+AGG - chr2_1:60840879-60840898 Msa0215550:intron 35.0%
!! TATTTTGGAGATGAGGAGAA+AGG - chr2_1:60841146-60841165 Msa0215550:CDS 35.0%
CTTCATACTCAAGATCACTT+AGG + chr2_1:60841200-60841219 None:intergenic 35.0%
CTTGAGTATGAAGAAGTTCA+AGG - chr2_1:60841207-60841226 Msa0215550:CDS 35.0%
GGAATTATGTTAGCTAAGCT+TGG + chr2_1:60841276-60841295 None:intergenic 35.0%
AGCTTAGCTAACATAATTCC+TGG - chr2_1:60841276-60841295 Msa0215550:CDS 35.0%
!! CTATTTTTCTCTTGCAAACC+AGG + chr2_1:60841297-60841316 None:intergenic 35.0%
! TTATTTCCCCAATTTGGAAC+AGG + chr2_1:60841399-60841418 None:intergenic 35.0%
! GACATCTTATTTCCCCAATT+TGG + chr2_1:60841405-60841424 None:intergenic 35.0%
TTAGTCATCCACAAAGTTGA+TGG + chr2_1:60841627-60841646 None:intergenic 35.0%
! TGAATCTACTTGAAGAGTGT+TGG - chr2_1:60839914-60839933 Msa0215550:exon 35.0%
GAATGACACCTTAACATCTT+TGG + chr2_1:60839967-60839986 None:intergenic 35.0%
!! ATTAGTCTTGTTGAAGGGTA+AGG + chr2_1:60839994-60840013 None:intergenic 35.0%
!! AGCTGATTAGTCTTGTTGAA+GGG + chr2_1:60839999-60840018 None:intergenic 35.0%
AGGATGAACAAAATCACCAT+CGG + chr2_1:60840058-60840077 None:intergenic 35.0%
TCTTCTTTCACTCTAATTGG+TGG + chr2_1:60840108-60840127 None:intergenic 35.0%
CAAATTGGTGCATCAAACAT+CGG - chr2_1:60840152-60840171 Msa0215550:CDS 35.0%
CATCATTGTGCAAAGATGAA+AGG - chr2_1:60840204-60840223 Msa0215550:CDS 35.0%
TACCAAAAAGAGAAGAATGC+AGG - chr2_1:60840871-60840890 Msa0215550:intron 35.0%
! TTCCTGCATTCTTCTCTTTT+TGG + chr2_1:60840876-60840895 None:intergenic 35.0%
AGAGAAGAATGCAGGAATAT+AGG - chr2_1:60840879-60840898 Msa0215550:intron 35.0%
!! TATTTTGGAGATGAGGAGAA+AGG - chr2_1:60841146-60841165 Msa0215550:CDS 35.0%
CTTCATACTCAAGATCACTT+AGG + chr2_1:60841200-60841219 None:intergenic 35.0%
CTTGAGTATGAAGAAGTTCA+AGG - chr2_1:60841207-60841226 Msa0215550:CDS 35.0%
GGAATTATGTTAGCTAAGCT+TGG + chr2_1:60841276-60841295 None:intergenic 35.0%
AGCTTAGCTAACATAATTCC+TGG - chr2_1:60841276-60841295 Msa0215550:CDS 35.0%
!! CTATTTTTCTCTTGCAAACC+AGG + chr2_1:60841297-60841316 None:intergenic 35.0%
! TTATTTCCCCAATTTGGAAC+AGG + chr2_1:60841399-60841418 None:intergenic 35.0%
! GACATCTTATTTCCCCAATT+TGG + chr2_1:60841405-60841424 None:intergenic 35.0%
TTAGTCATCCACAAAGTTGA+TGG + chr2_1:60841627-60841646 None:intergenic 35.0%
!! GAGCTGATTAGTCTTGTTGA+AGG + chr2_1:60840000-60840019 None:intergenic 40.0%
TGAACAAAATCACCATCGGA+TGG + chr2_1:60840054-60840073 None:intergenic 40.0%
! ACTCTAATTGGTGGCATAGA+TGG + chr2_1:60840099-60840118 None:intergenic 40.0%
ATCGGATTCTGCTATGCATA+AGG - chr2_1:60840170-60840189 Msa0215550:CDS 40.0%
CATCTTTGCCTCCAACAATA+GGG - chr2_1:60840280-60840299 Msa0215550:CDS 40.0%
! TTTTCCTCTCTCCCTATTGT+TGG + chr2_1:60840294-60840313 None:intergenic 40.0%
GGAGGCAAGTTGTCATTATT+GGG + chr2_1:60840372-60840391 None:intergenic 40.0%
TGGAGGCAAGTTGTCATTAT+TGG + chr2_1:60840373-60840392 None:intergenic 40.0%
ATAATGACAACTTGCCTCCA+AGG - chr2_1:60840373-60840392 Msa0215550:intron 40.0%
AGTACCTTAGAGGTTAACCT+TGG + chr2_1:60840393-60840412 None:intergenic 40.0%
TCGTTTATGATTAAGCGCGT+AGG - chr2_1:60840467-60840486 Msa0215550:intron 40.0%
ATATGGCAAGTAGTCTGGTA+CGG - chr2_1:60840540-60840559 Msa0215550:intron 40.0%
!! TTTTTAGCCGGTCTACTTCT+TGG + chr2_1:60841096-60841115 None:intergenic 40.0%
!!! AAGAGGGTATTTTGGAGATG+AGG - chr2_1:60841139-60841158 Msa0215550:CDS 40.0%
TGAAGAAGTTCAAGGGTTCA+AGG - chr2_1:60841215-60841234 Msa0215550:CDS 40.0%
AAGAGGACCTTACTTATCAG+AGG - chr2_1:60841344-60841363 Msa0215550:CDS 40.0%
AACCATGCCTCTGATAAGTA+AGG + chr2_1:60841354-60841373 None:intergenic 40.0%
! TTACTTATCAGAGGCATGGT+TGG - chr2_1:60841353-60841372 Msa0215550:CDS 40.0%
AACTCCTCCTGTTCCAAATT+GGG - chr2_1:60841389-60841408 Msa0215550:CDS 40.0%
! CGAAGTATCCATCAACTTTG+TGG - chr2_1:60841616-60841635 Msa0215550:five_prime_UTR 40.0%
!! GAGCTGATTAGTCTTGTTGA+AGG + chr2_1:60840000-60840019 None:intergenic 40.0%
TGAACAAAATCACCATCGGA+TGG + chr2_1:60840054-60840073 None:intergenic 40.0%
! ACTCTAATTGGTGGCATAGA+TGG + chr2_1:60840099-60840118 None:intergenic 40.0%
ATCGGATTCTGCTATGCATA+AGG - chr2_1:60840170-60840189 Msa0215550:CDS 40.0%
CATCTTTGCCTCCAACAATA+GGG - chr2_1:60840280-60840299 Msa0215550:CDS 40.0%
! TTTTCCTCTCTCCCTATTGT+TGG + chr2_1:60840294-60840313 None:intergenic 40.0%
GGAGGCAAGTTGTCATTATT+GGG + chr2_1:60840372-60840391 None:intergenic 40.0%
TGGAGGCAAGTTGTCATTAT+TGG + chr2_1:60840373-60840392 None:intergenic 40.0%
ATAATGACAACTTGCCTCCA+AGG - chr2_1:60840373-60840392 Msa0215550:intron 40.0%
AGTACCTTAGAGGTTAACCT+TGG + chr2_1:60840393-60840412 None:intergenic 40.0%
TCGTTTATGATTAAGCGCGT+AGG - chr2_1:60840467-60840486 Msa0215550:intron 40.0%
ATATGGCAAGTAGTCTGGTA+CGG - chr2_1:60840540-60840559 Msa0215550:intron 40.0%
!! TTTTTAGCCGGTCTACTTCT+TGG + chr2_1:60841096-60841115 None:intergenic 40.0%
!!! AAGAGGGTATTTTGGAGATG+AGG - chr2_1:60841139-60841158 Msa0215550:CDS 40.0%
TGAAGAAGTTCAAGGGTTCA+AGG - chr2_1:60841215-60841234 Msa0215550:CDS 40.0%
AAGAGGACCTTACTTATCAG+AGG - chr2_1:60841344-60841363 Msa0215550:CDS 40.0%
AACCATGCCTCTGATAAGTA+AGG + chr2_1:60841354-60841373 None:intergenic 40.0%
! TTACTTATCAGAGGCATGGT+TGG - chr2_1:60841353-60841372 Msa0215550:CDS 40.0%
AACTCCTCCTGTTCCAAATT+GGG - chr2_1:60841389-60841408 Msa0215550:CDS 40.0%
! CGAAGTATCCATCAACTTTG+TGG - chr2_1:60841616-60841635 Msa0215550:five_prime_UTR 40.0%
TCAGCAAGTAAACCATCCGA+TGG - chr2_1:60840039-60840058 Msa0215550:CDS 45.0%
GATTCTGCTATGCATAAGGC+AGG - chr2_1:60840174-60840193 Msa0215550:CDS 45.0%
CCTATTGTTGGAGGCAAAGA+TGG + chr2_1:60840282-60840301 None:intergenic 45.0%
CCATCTTTGCCTCCAACAAT+AGG - chr2_1:60840279-60840298 Msa0215550:CDS 45.0%
!!! AGGTGTCGATGGTTGTTTTC+GGG + chr2_1:60840349-60840368 None:intergenic 45.0%
!!! GAGGTGTCGATGGTTGTTTT+CGG + chr2_1:60840350-60840369 None:intergenic 45.0%
!! TCATTATTGGGAGGTGTCGA+TGG + chr2_1:60840360-60840379 None:intergenic 45.0%
GGCAAGTTGTCATTATTGGG+AGG + chr2_1:60840369-60840388 None:intergenic 45.0%
ACCTTAGAGGTTAACCTTGG+AGG + chr2_1:60840390-60840409 None:intergenic 45.0%
CAGCATTCCAAGAAGTAGAC+CGG - chr2_1:60841086-60841105 Msa0215550:CDS 45.0%
GTTCAAGGGTTCAAGGACTT+AGG - chr2_1:60841222-60841241 Msa0215550:CDS 45.0%
GAGGAAGATAAAGCAGCAAG+AGG - chr2_1:60841327-60841346 Msa0215550:CDS 45.0%
GACCTTACTTATCAGAGGCA+TGG - chr2_1:60841349-60841368 Msa0215550:CDS 45.0%
CAACTCCTCCTGTTCCAAAT+TGG - chr2_1:60841388-60841407 Msa0215550:CDS 45.0%
ACTCCTCCTGTTCCAAATTG+GGG - chr2_1:60841390-60841409 Msa0215550:CDS 45.0%
! TTTCCCCAATTTGGAACAGG+AGG + chr2_1:60841396-60841415 None:intergenic 45.0%
ATCTCCGTCGAAAACCTAGT+TGG - chr2_1:60841646-60841665 Msa0215550:five_prime_UTR 45.0%
TCAGCAAGTAAACCATCCGA+TGG - chr2_1:60840039-60840058 Msa0215550:CDS 45.0%
GATTCTGCTATGCATAAGGC+AGG - chr2_1:60840174-60840193 Msa0215550:CDS 45.0%
CCTATTGTTGGAGGCAAAGA+TGG + chr2_1:60840282-60840301 None:intergenic 45.0%
CCATCTTTGCCTCCAACAAT+AGG - chr2_1:60840279-60840298 Msa0215550:CDS 45.0%
!!! AGGTGTCGATGGTTGTTTTC+GGG + chr2_1:60840349-60840368 None:intergenic 45.0%
!!! GAGGTGTCGATGGTTGTTTT+CGG + chr2_1:60840350-60840369 None:intergenic 45.0%
!! TCATTATTGGGAGGTGTCGA+TGG + chr2_1:60840360-60840379 None:intergenic 45.0%
GGCAAGTTGTCATTATTGGG+AGG + chr2_1:60840369-60840388 None:intergenic 45.0%
ACCTTAGAGGTTAACCTTGG+AGG + chr2_1:60840390-60840409 None:intergenic 45.0%
CAGCATTCCAAGAAGTAGAC+CGG - chr2_1:60841086-60841105 Msa0215550:CDS 45.0%
GTTCAAGGGTTCAAGGACTT+AGG - chr2_1:60841222-60841241 Msa0215550:CDS 45.0%
GAGGAAGATAAAGCAGCAAG+AGG - chr2_1:60841327-60841346 Msa0215550:CDS 45.0%
GACCTTACTTATCAGAGGCA+TGG - chr2_1:60841349-60841368 Msa0215550:CDS 45.0%
CAACTCCTCCTGTTCCAAAT+TGG - chr2_1:60841388-60841407 Msa0215550:CDS 45.0%
ACTCCTCCTGTTCCAAATTG+GGG - chr2_1:60841390-60841409 Msa0215550:CDS 45.0%
! TTTCCCCAATTTGGAACAGG+AGG + chr2_1:60841396-60841415 None:intergenic 45.0%
ATCTCCGTCGAAAACCTAGT+TGG - chr2_1:60841646-60841665 Msa0215550:five_prime_UTR 45.0%
GGTGCTCTTTGCAGCTTCTT+AGG + chr2_1:60840078-60840097 None:intergenic 50.0%
TCCTCTCTCCCTATTGTTGG+AGG + chr2_1:60840291-60840310 None:intergenic 50.0%
GCCTCCAAGGTTAACCTCTA+AGG - chr2_1:60840386-60840405 Msa0215550:intron 50.0%
GTCTGGTACGGTTCCAATCA+CGG - chr2_1:60840552-60840571 Msa0215550:intron 50.0%
AACTGCAACGTGACCGTGAT+TGG + chr2_1:60840568-60840587 None:intergenic 50.0%
!! TGGTGTTACTTCTTGGTGCC+AGG + chr2_1:60840673-60840692 None:intergenic 50.0%
!!! CAAGTTTTGGGCTAGAGCTG+TGG - chr2_1:60841449-60841468 Msa0215550:CDS 50.0%
GGTGCTCTTTGCAGCTTCTT+AGG + chr2_1:60840078-60840097 None:intergenic 50.0%
TCCTCTCTCCCTATTGTTGG+AGG + chr2_1:60840291-60840310 None:intergenic 50.0%
GCCTCCAAGGTTAACCTCTA+AGG - chr2_1:60840386-60840405 Msa0215550:intron 50.0%
GTCTGGTACGGTTCCAATCA+CGG - chr2_1:60840552-60840571 Msa0215550:intron 50.0%
AACTGCAACGTGACCGTGAT+TGG + chr2_1:60840568-60840587 None:intergenic 50.0%
!! TGGTGTTACTTCTTGGTGCC+AGG + chr2_1:60840673-60840692 None:intergenic 50.0%
!!! CAAGTTTTGGGCTAGAGCTG+TGG - chr2_1:60841449-60841468 Msa0215550:CDS 50.0%
GCCTCCAACAATAGGGAGAG+AGG - chr2_1:60840287-60840306 Msa0215550:CDS 55.0%
CACGGTCACGTTGCAGTTAC+AGG - chr2_1:60840570-60840589 Msa0215550:intron 55.0%
GCCTCCAACAATAGGGAGAG+AGG - chr2_1:60840287-60840306 Msa0215550:CDS 55.0%
CACGGTCACGTTGCAGTTAC+AGG - chr2_1:60840570-60840589 Msa0215550:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 60839751 60841669 60839751 ID=Msa0215550;Name=Msa0215550
chr2_1 mRNA 60839751 60841669 60839751 ID=Msa0215550-mRNA-1;Parent=Msa0215550;Name=Msa0215550-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|181|322
chr2_1 exon 60839751 60840339 60839751 ID=Msa0215550-mRNA-1:exon:16722;Parent=Msa0215550-mRNA-1
chr2_1 exon 60841013 60841669 60841013 ID=Msa0215550-mRNA-1:exon:16721;Parent=Msa0215550-mRNA-1
chr2_1 five_prime_UTR 60841574 60841669 60841574 ID=Msa0215550-mRNA-1:five_prime_utr;Parent=Msa0215550-mRNA-1
chr2_1 CDS 60841013 60841573 60841013 ID=Msa0215550-mRNA-1:cds;Parent=Msa0215550-mRNA-1
chr2_1 CDS 60839932 60840339 60839932 ID=Msa0215550-mRNA-1:cds;Parent=Msa0215550-mRNA-1
chr2_1 three_prime_UTR 60839751 60839931 60839751 ID=Msa0215550-mRNA-1:three_prime_utr;Parent=Msa0215550-mRNA-1
Gene Sequence

>Msa0215550

ATGGATCCAAATGATAATAATAGCTCTTGTGAATCTTCTTTTTGCAAAGATGAAAAAGTGGAAGCAATGAATCTACTTGAAGAGTGTTGGTACTTTGATAACTTGTTAAATATAAAACCAAAGATGTTAAGGTGTCATTCTGATCCTTACCCTTCAACAAGACTAATCAGCTCAGAATTTTTGAAGGATTCATCAGCAAGTAAACCATCCGATGGTGATTTTGTTCATCCTAAGAAGCTGCAAAGAGCACCATCTATGCCACCAATTAGAGTGAAAGAAGAAGAAAAAGATGATAAAAAAGGAAGCAAATTGGTGCATCAAACATCGGATTCTGCTATGCATAAGGCAGGTAGTAAACATCATTGTGCAAAGATGAAAGGACTTGATCATAGAAGTGATAGCAATAGAAGGAAAAGCAAATTGCTAAGAACACCATCTTTGCCTCCAACAATAGGGAGAGAGGAAAAGTTTCAAGAAACTGATTCAAGAATTGGAAGATCCCGAAAACAACCATCGACACCTCCCAATAATGACAACTTGCCTCCAAGGTTAACCTCTAAGAGTTGCAGCATTCCAAGAAGTAGACCGGCTAAAAACATCGAAATTGAAAGCTTAAACAAAGAGGGTATTTTGGAGATGAGGAGAAAGGTCCTAAATCAGAAAACAATAAGGAGAAGCCTAAGTGATCTTGAGTATGAAGAAGTTCAAGGGTTCAAGGACTTAGGATTCTCATTTGAGAAAGAAGCTTTAAGTCCAAGCTTAGCTAACATAATTCCTGGTTTGCAAGAGAAAAATAGAGATGAAAGTGAGGAAGATAAAGCAGCAAGAGGACCTTACTTATCAGAGGCATGGTTGGTGCAAAGTTGTGCAACTCCTCCTGTTCCAAATTGGGGAAATAAGATGTCTGCAGATGACATGAAAAAACATATCAAGTTTTGGGCTAGAGCTGTGGCTTCAACTAAATGTTGA
Protein sequence

>Msa0215550

MDPNDNNSSCESSFCKDEKVEAMNLLEECWYFDNLLNIKPKMLRCHSDPYPSTRLISSEFLKDSSASKPSDGDFVHPKKLQRAPSMPPIRVKEEEKDDKKGSKLVHQTSDSAMHKAGSKHHCAKMKGLDHRSDSNRRKSKLLRTPSLPPTIGREEKFQETDSRIGRSRKQPSTPPNNDNLPPRLTSKSCSIPRSRPAKNIEIESLNKEGILEMRRKVLNQKTIRRSLSDLEYEEVQGFKDLGFSFEKEALSPSLANIIPGLQEKNRDESEEDKAARGPYLSEAWLVQSCATPPVPNWGNKMSADDMKKHIKFWARAVASTKC*