AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0333390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0333390 MtrunA17_Chr2g0316841 97.826 322 7 0 1 322 1 322 0.0 648
Msa0333390 MtrunA17_Chr2g0319971 47.059 102 42 5 225 319 134 230 3.85e-18 82.4
Msa0333390 MtrunA17_Chr2g0284311 39.053 169 83 5 167 320 66 229 4.88e-18 81.6
Msa0333390 MtrunA17_Chr8g0379321 41.322 121 52 5 216 320 123 240 9.58e-17 78.2
Msa0333390 MtrunA17_Chr4g0041451 40.367 109 47 3 229 319 128 236 5.18e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0333390 AT3G15115.1 30.000 350 169 16 18 319 12 333 7.22e-20 89.0

Find 65 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCTTATTTCCCCAATT+TGG 0.135591 2_4:+51113661 None:intergenic
TCTTCTTCTTTCACTCTAAT+TGG 0.176697 2_4:+51114971 None:intergenic
CAACTCCTCCTGTTCCAAAT+TGG 0.187842 2_4:-51113675 Msa0333390:CDS
CTTAAACAAAGAGGGTATTT+TGG 0.192411 2_4:-51113932 Msa0333390:CDS
TGGAGGCAAGTTGTCATTAT+TGG 0.201109 2_4:+51114709 None:intergenic
AGTTCAAGGGTTCAAGGATT+TGG 0.219405 2_4:-51113842 Msa0333390:CDS
GTATTCTGAATTTATATATA+TGG 0.237258 2_4:+51115302 None:intergenic
GAATGACATCTTAACATCTT+TGG 0.252145 2_4:+51115115 None:intergenic
GGTGCTCTTTGCAGCTTCTT+AGG 0.254314 2_4:+51115004 None:intergenic
CAAGAAACTGATTCAAGAAT+TGG 0.268407 2_4:-51114761 Msa0333390:CDS
AACTCCTCCTGTTCCAAATT+GGG 0.290556 2_4:-51113674 Msa0333390:CDS
GTTCAAGGGTTCAAGGATTT+GGG 0.318307 2_4:-51113841 Msa0333390:CDS
GGAGGCAAGTTGTCATTATT+GGG 0.324183 2_4:+51114710 None:intergenic
CAAGAGCTATTATTATCATT+TGG 0.336403 2_4:+51115226 None:intergenic
CGCATCTATTATCTATAGTT+AGG 0.337594 2_4:+51113468 None:intergenic
ACTCTAATTGGTGGCATAGA+TGG 0.345119 2_4:+51114983 None:intergenic
CCATCTTTGCCTCCAACAAT+AGG 0.347224 2_4:-51114800 Msa0333390:CDS
AGCTTAGCTAACATAATTCC+TGG 0.370469 2_4:-51113787 Msa0333390:CDS
TATTCTGAATTTATATATAT+GGG 0.384564 2_4:+51115303 None:intergenic
GCCTCCAAGGTTAACCTCTA+AGG 0.388438 2_4:-51114693 Msa0333390:intron
TCCTCTCTCCCTATTGTTGG+AGG 0.400398 2_4:+51114791 None:intergenic
TTAGTCATCCACAAAGTTGA+TGG 0.405237 2_4:+51113439 None:intergenic
TCCTAAATCAGAAAACAATA+AGG 0.414045 2_4:-51113894 Msa0333390:CDS
CGAAGTATCCATCAACTTTG+TGG 0.415896 2_4:-51113447 Msa0333390:three_prime_UTR
TACATACATACAAACATGTA+AGG 0.423768 2_4:-51115282 Msa0333390:five_prime_UTR
TTATTTCCCCAATTTGGAAC+AGG 0.427610 2_4:+51113667 None:intergenic
GGAATTATGTTAGCTAAGCT+TGG 0.440726 2_4:+51113790 None:intergenic
TCTTCTTTCACTCTAATTGG+TGG 0.448745 2_4:+51114974 None:intergenic
CCTATTGTTGGAGGCAAAGA+TGG 0.449300 2_4:+51114800 None:intergenic
CCCACTAAAGGTGGCCAACT+AGG 0.457150 2_4:+51113402 None:intergenic
CTTGAGTATGAAGAAGTTCA+AGG 0.460785 2_4:-51113856 Msa0333390:CDS
ATAGAAGTGATAGCAATAGA+AGG 0.463972 2_4:-51114844 Msa0333390:CDS
ATTAGTCTTGTTGAAGGGTA+AGG 0.475567 2_4:+51115088 None:intergenic
CATCTTTGCCTCCAACAATA+GGG 0.480386 2_4:-51114799 Msa0333390:CDS
ACCTTAGAGGTTAACCTTGG+AGG 0.482914 2_4:+51114692 None:intergenic
GAGCTGATTAGTCTTGTTGA+AGG 0.486253 2_4:+51115082 None:intergenic
ATCGGATTCTGCTATGCATA+AGG 0.492617 2_4:-51114909 Msa0333390:CDS
TGAAGAAGTTCAAGGGTTCA+AGG 0.522733 2_4:-51113848 Msa0333390:CDS
AGTACCTTAGAGGTTAACCT+TGG 0.525551 2_4:+51114689 None:intergenic
AACCATGCCTCTGATAAGTA+AGG 0.533103 2_4:+51113712 None:intergenic
TCATTATTGGGAGGTGTCGA+TGG 0.539813 2_4:+51114722 None:intergenic
ATAATGACAACTTGCCTCCA+AGG 0.544515 2_4:-51114706 Msa0333390:CDS
TGAATCTACTTGAAGAGTGT+TGG 0.552194 2_4:-51115165 Msa0333390:CDS
TTACTTATCAGAGGCATGGT+TGG 0.560586 2_4:-51113710 Msa0333390:CDS
CATCATTGTGCAAAGATGAA+AGG 0.561895 2_4:-51114875 Msa0333390:CDS
ACTCCTCCTGTTCCAAATTG+GGG 0.566945 2_4:-51113673 Msa0333390:CDS
CTTCATACTCAAGATCACTT+AGG 0.571003 2_4:+51113866 None:intergenic
CAGCATTCCAAGAAGTAGAC+CGG 0.572646 2_4:-51113977 Msa0333390:CDS
GACCTTACTTATCAGAGGCA+TGG 0.573969 2_4:-51113714 Msa0333390:CDS
GTTGAAAGCTTAAACAAAGA+GGG 0.576048 2_4:-51113940 Msa0333390:CDS
AGCTGATTAGTCTTGTTGAA+GGG 0.577448 2_4:+51115083 None:intergenic
TTTCCCCAATTTGGAACAGG+AGG 0.577483 2_4:+51113670 None:intergenic
GATTCTGCTATGCATAAGGC+AGG 0.577729 2_4:-51114905 Msa0333390:CDS
GCCTCCAACAATAGGGAGAG+AGG 0.599344 2_4:-51114792 Msa0333390:CDS
AGTTGAAAGCTTAAACAAAG+AGG 0.608171 2_4:-51113941 Msa0333390:CDS
TTGAGTATGAAGAAGTTCAA+GGG 0.610506 2_4:-51113855 Msa0333390:CDS
GAGGAAGATAAAGCAGCAAG+AGG 0.618815 2_4:-51113736 Msa0333390:CDS
ACATACATACAAACATGTAA+GGG 0.620573 2_4:-51115281 Msa0333390:five_prime_UTR
CAAATTGGTGCATCAAACAT+CGG 0.624498 2_4:-51114927 Msa0333390:CDS
GGCAAGTTGTCATTATTGGG+AGG 0.626871 2_4:+51114713 None:intergenic
AGGATGAACAAAATCACCAT+CGG 0.638726 2_4:+51115024 None:intergenic
GTTAGAAAAGTTGAAACACA+TGG 0.639189 2_4:-51115251 Msa0333390:exon
AAGAGGACCTTACTTATCAG+AGG 0.645280 2_4:-51113719 Msa0333390:CDS
TGAACAAAATCACCATCGGA+TGG 0.647374 2_4:+51115028 None:intergenic
TCAGCAAGTAAACCATCCGA+TGG 0.664328 2_4:-51115040 Msa0333390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTGAATTTATATATAT+GGG + chr2_4:51113436-51113455 None:intergenic 10.0%
!! TATTCTGAATTTATATATAT+GGG + chr2_4:51113436-51113455 None:intergenic 10.0%
!! GTATTCTGAATTTATATATA+TGG + chr2_4:51113437-51113456 None:intergenic 15.0%
!! ACTTAAATGCTAAAATTATT+TGG + chr2_4:51114377-51114396 None:intergenic 15.0%
!! GTATTCTGAATTTATATATA+TGG + chr2_4:51113437-51113456 None:intergenic 15.0%
!! ACTTAAATGCTAAAATTATT+TGG + chr2_4:51114377-51114396 None:intergenic 15.0%
!! GAAGAAAAAGATGATAAAAA+AGG - chr2_4:51113783-51113802 Msa0333390:CDS 20.0%
!! GACATAATGATTTAAAAAGA+GGG + chr2_4:51114276-51114295 None:intergenic 20.0%
!! TGACATAATGATTTAAAAAG+AGG + chr2_4:51114277-51114296 None:intergenic 20.0%
!! TAATATCAATCATAAACTCT+TGG + chr2_4:51114303-51114322 None:intergenic 20.0%
!! AACACCAATTAATACAAATA+TGG - chr2_4:51114343-51114362 Msa0333390:intron 20.0%
!! TTAGCCATATTTGTATTAAT+TGG + chr2_4:51114350-51114369 None:intergenic 20.0%
!! TTAATACAAATATGGCTAAA+AGG - chr2_4:51114351-51114370 Msa0333390:intron 20.0%
!! ATGATCAATCAAAATATGAT+TGG + chr2_4:51114571-51114590 None:intergenic 20.0%
!!! TGAAAAAACATATCAAGTTT+TGG - chr2_4:51115109-51115128 Msa0333390:CDS 20.0%
!!! GAAAAAACATATCAAGTTTT+GGG - chr2_4:51115110-51115129 Msa0333390:CDS 20.0%
!!! TTATTCTTTTGATTGTTCTT+AGG + chr2_4:51115216-51115235 None:intergenic 20.0%
!! GAAGAAAAAGATGATAAAAA+AGG - chr2_4:51113783-51113802 Msa0333390:CDS 20.0%
!! GACATAATGATTTAAAAAGA+GGG + chr2_4:51114276-51114295 None:intergenic 20.0%
!! TGACATAATGATTTAAAAAG+AGG + chr2_4:51114277-51114296 None:intergenic 20.0%
!! TAATATCAATCATAAACTCT+TGG + chr2_4:51114303-51114322 None:intergenic 20.0%
!! AACACCAATTAATACAAATA+TGG - chr2_4:51114343-51114362 Msa0333390:intron 20.0%
!! TTAGCCATATTTGTATTAAT+TGG + chr2_4:51114350-51114369 None:intergenic 20.0%
!! TTAATACAAATATGGCTAAA+AGG - chr2_4:51114351-51114370 Msa0333390:intron 20.0%
!! ATGATCAATCAAAATATGAT+TGG + chr2_4:51114571-51114590 None:intergenic 20.0%
!!! TGAAAAAACATATCAAGTTT+TGG - chr2_4:51115109-51115128 Msa0333390:CDS 20.0%
!!! GAAAAAACATATCAAGTTTT+GGG - chr2_4:51115110-51115129 Msa0333390:CDS 20.0%
!!! TTATTCTTTTGATTGTTCTT+AGG + chr2_4:51115216-51115235 None:intergenic 20.0%
! TACATACATACAAACATGTA+AGG - chr2_4:51113454-51113473 Msa0333390:three_prime_UTR 25.0%
! ACATACATACAAACATGTAA+GGG - chr2_4:51113455-51113474 Msa0333390:three_prime_UTR 25.0%
! CAAGAGCTATTATTATCATT+TGG + chr2_4:51113513-51113532 None:intergenic 25.0%
!! TTTTTGCAAAGATGAAAAAG+TGG - chr2_4:51113542-51113561 Msa0333390:three_prime_UTR 25.0%
! TGATAAAAAAGGAAGCAAAT+TGG - chr2_4:51113794-51113813 Msa0333390:CDS 25.0%
!! TGATTTTTATAGTACCTTAG+AGG + chr2_4:51114060-51114079 None:intergenic 25.0%
! CGAAGAATTGTGATTTAATA+TGG - chr2_4:51114179-51114198 Msa0333390:intron 25.0%
! TAATGATTTAAAAAGAGGGT+AGG + chr2_4:51114272-51114291 None:intergenic 25.0%
!!! TATTAATTGGTGTTACTTCT+TGG + chr2_4:51114337-51114356 None:intergenic 25.0%
! ATAGCATCAGAAATAGAAAA+GGG - chr2_4:51114403-51114422 Msa0333390:intron 25.0%
! ATATTTGAACATAGCATGAT+AGG + chr2_4:51114478-51114497 None:intergenic 25.0%
! TCCTAAATCAGAAAACAATA+AGG - chr2_4:51114842-51114861 Msa0333390:CDS 25.0%
!! TCCTTATTGTTTTCTGATTT+AGG + chr2_4:51114846-51114865 None:intergenic 25.0%
! TACATACATACAAACATGTA+AGG - chr2_4:51113454-51113473 Msa0333390:three_prime_UTR 25.0%
! ACATACATACAAACATGTAA+GGG - chr2_4:51113455-51113474 Msa0333390:three_prime_UTR 25.0%
! CAAGAGCTATTATTATCATT+TGG + chr2_4:51113513-51113532 None:intergenic 25.0%
!! TTTTTGCAAAGATGAAAAAG+TGG - chr2_4:51113542-51113561 Msa0333390:three_prime_UTR 25.0%
! TGATAAAAAAGGAAGCAAAT+TGG - chr2_4:51113794-51113813 Msa0333390:CDS 25.0%
!! TGATTTTTATAGTACCTTAG+AGG + chr2_4:51114060-51114079 None:intergenic 25.0%
! CGAAGAATTGTGATTTAATA+TGG - chr2_4:51114179-51114198 Msa0333390:intron 25.0%
! TAATGATTTAAAAAGAGGGT+AGG + chr2_4:51114272-51114291 None:intergenic 25.0%
!!! TATTAATTGGTGTTACTTCT+TGG + chr2_4:51114337-51114356 None:intergenic 25.0%
! ATAGCATCAGAAATAGAAAA+GGG - chr2_4:51114403-51114422 Msa0333390:intron 25.0%
! ATATTTGAACATAGCATGAT+AGG + chr2_4:51114478-51114497 None:intergenic 25.0%
! TCCTAAATCAGAAAACAATA+AGG - chr2_4:51114842-51114861 Msa0333390:CDS 25.0%
!! TCCTTATTGTTTTCTGATTT+AGG + chr2_4:51114846-51114865 None:intergenic 25.0%
GTTAGAAAAGTTGAAACACA+TGG - chr2_4:51113485-51113504 Msa0333390:three_prime_UTR 30.0%
GAATGACATCTTAACATCTT+TGG + chr2_4:51113624-51113643 None:intergenic 30.0%
TCTTCTTCTTTCACTCTAAT+TGG + chr2_4:51113768-51113787 None:intergenic 30.0%
ATAGAAGTGATAGCAATAGA+AGG - chr2_4:51113892-51113911 Msa0333390:CDS 30.0%
! CAAGAAACTGATTCAAGAAT+TGG - chr2_4:51113975-51113994 Msa0333390:CDS 30.0%
ATTTAATATGGCAAGTAGTC+TGG - chr2_4:51114191-51114210 Msa0333390:intron 30.0%
CATAGCATCAGAAATAGAAA+AGG - chr2_4:51114402-51114421 Msa0333390:intron 30.0%
ACAAAAAAAGAGAAGAATGC+AGG - chr2_4:51114529-51114548 Msa0333390:intron 30.0%
ATGCAACAATAATACGTGAA+AGG + chr2_4:51114613-51114632 None:intergenic 30.0%
AATATCGCTTATTGTTACGA+AGG + chr2_4:51114647-51114666 None:intergenic 30.0%
AGTTGAAAGCTTAAACAAAG+AGG - chr2_4:51114795-51114814 Msa0333390:CDS 30.0%
GTTGAAAGCTTAAACAAAGA+GGG - chr2_4:51114796-51114815 Msa0333390:CDS 30.0%
!!! CTTAAACAAAGAGGGTATTT+TGG - chr2_4:51114804-51114823 Msa0333390:CDS 30.0%
TTGAGTATGAAGAAGTTCAA+GGG - chr2_4:51114881-51114900 Msa0333390:CDS 30.0%
GAAAAATAGAGATGAAAGTG+AGG - chr2_4:51114981-51115000 Msa0333390:CDS 30.0%
CGCATCTATTATCTATAGTT+AGG + chr2_4:51115271-51115290 None:intergenic 30.0%
GTTAGAAAAGTTGAAACACA+TGG - chr2_4:51113485-51113504 Msa0333390:three_prime_UTR 30.0%
GAATGACATCTTAACATCTT+TGG + chr2_4:51113624-51113643 None:intergenic 30.0%
TCTTCTTCTTTCACTCTAAT+TGG + chr2_4:51113768-51113787 None:intergenic 30.0%
ATAGAAGTGATAGCAATAGA+AGG - chr2_4:51113892-51113911 Msa0333390:CDS 30.0%
! CAAGAAACTGATTCAAGAAT+TGG - chr2_4:51113975-51113994 Msa0333390:CDS 30.0%
ATTTAATATGGCAAGTAGTC+TGG - chr2_4:51114191-51114210 Msa0333390:intron 30.0%
CATAGCATCAGAAATAGAAA+AGG - chr2_4:51114402-51114421 Msa0333390:intron 30.0%
ACAAAAAAAGAGAAGAATGC+AGG - chr2_4:51114529-51114548 Msa0333390:intron 30.0%
ATGCAACAATAATACGTGAA+AGG + chr2_4:51114613-51114632 None:intergenic 30.0%
AATATCGCTTATTGTTACGA+AGG + chr2_4:51114647-51114666 None:intergenic 30.0%
AGTTGAAAGCTTAAACAAAG+AGG - chr2_4:51114795-51114814 Msa0333390:CDS 30.0%
GTTGAAAGCTTAAACAAAGA+GGG - chr2_4:51114796-51114815 Msa0333390:CDS 30.0%
!!! CTTAAACAAAGAGGGTATTT+TGG - chr2_4:51114804-51114823 Msa0333390:CDS 30.0%
TTGAGTATGAAGAAGTTCAA+GGG - chr2_4:51114881-51114900 Msa0333390:CDS 30.0%
GAAAAATAGAGATGAAAGTG+AGG - chr2_4:51114981-51115000 Msa0333390:CDS 30.0%
CGCATCTATTATCTATAGTT+AGG + chr2_4:51115271-51115290 None:intergenic 30.0%
! TGAATCTACTTGAAGAGTGT+TGG - chr2_4:51113571-51113590 Msa0333390:three_prime_UTR 35.0%
!! ATTAGTCTTGTTGAAGGGTA+AGG + chr2_4:51113651-51113670 None:intergenic 35.0%
!! AGCTGATTAGTCTTGTTGAA+GGG + chr2_4:51113656-51113675 None:intergenic 35.0%
! AATCAGCTCAGAACTTTTGA+AGG - chr2_4:51113668-51113687 Msa0333390:CDS 35.0%
AGGATGAACAAAATCACCAT+CGG + chr2_4:51113715-51113734 None:intergenic 35.0%
TCTTCTTTCACTCTAATTGG+TGG + chr2_4:51113765-51113784 None:intergenic 35.0%
CAAATTGGTGCATCAAACAT+CGG - chr2_4:51113809-51113828 Msa0333390:CDS 35.0%
CATCATTGTGCAAAGATGAA+AGG - chr2_4:51113861-51113880 Msa0333390:CDS 35.0%
AGAGAAGAATGCAGGAATAT+AGG - chr2_4:51114537-51114556 Msa0333390:intron 35.0%
!!! TCAACTTCGATGTTTTTAGC+CGG + chr2_4:51114781-51114800 None:intergenic 35.0%
!! TATTTTGGAGATGAGGAGAA+AGG - chr2_4:51114819-51114838 Msa0333390:CDS 35.0%
CTTCATACTCAAGATCACTT+AGG + chr2_4:51114873-51114892 None:intergenic 35.0%
CTTGAGTATGAAGAAGTTCA+AGG - chr2_4:51114880-51114899 Msa0333390:CDS 35.0%
GGAATTATGTTAGCTAAGCT+TGG + chr2_4:51114949-51114968 None:intergenic 35.0%
AGCTTAGCTAACATAATTCC+TGG - chr2_4:51114949-51114968 Msa0333390:CDS 35.0%
!! CTATTTTTCTCTTGCAAACC+AGG + chr2_4:51114970-51114989 None:intergenic 35.0%
! TTATTTCCCCAATTTGGAAC+AGG + chr2_4:51115072-51115091 None:intergenic 35.0%
! GACATCTTATTTCCCCAATT+TGG + chr2_4:51115078-51115097 None:intergenic 35.0%
TTAGTCATCCACAAAGTTGA+TGG + chr2_4:51115300-51115319 None:intergenic 35.0%
! TGAATCTACTTGAAGAGTGT+TGG - chr2_4:51113571-51113590 Msa0333390:three_prime_UTR 35.0%
!! ATTAGTCTTGTTGAAGGGTA+AGG + chr2_4:51113651-51113670 None:intergenic 35.0%
!! AGCTGATTAGTCTTGTTGAA+GGG + chr2_4:51113656-51113675 None:intergenic 35.0%
! AATCAGCTCAGAACTTTTGA+AGG - chr2_4:51113668-51113687 Msa0333390:CDS 35.0%
AGGATGAACAAAATCACCAT+CGG + chr2_4:51113715-51113734 None:intergenic 35.0%
TCTTCTTTCACTCTAATTGG+TGG + chr2_4:51113765-51113784 None:intergenic 35.0%
CAAATTGGTGCATCAAACAT+CGG - chr2_4:51113809-51113828 Msa0333390:CDS 35.0%
CATCATTGTGCAAAGATGAA+AGG - chr2_4:51113861-51113880 Msa0333390:CDS 35.0%
AGAGAAGAATGCAGGAATAT+AGG - chr2_4:51114537-51114556 Msa0333390:intron 35.0%
!!! TCAACTTCGATGTTTTTAGC+CGG + chr2_4:51114781-51114800 None:intergenic 35.0%
!! TATTTTGGAGATGAGGAGAA+AGG - chr2_4:51114819-51114838 Msa0333390:CDS 35.0%
CTTCATACTCAAGATCACTT+AGG + chr2_4:51114873-51114892 None:intergenic 35.0%
CTTGAGTATGAAGAAGTTCA+AGG - chr2_4:51114880-51114899 Msa0333390:CDS 35.0%
GGAATTATGTTAGCTAAGCT+TGG + chr2_4:51114949-51114968 None:intergenic 35.0%
AGCTTAGCTAACATAATTCC+TGG - chr2_4:51114949-51114968 Msa0333390:CDS 35.0%
!! CTATTTTTCTCTTGCAAACC+AGG + chr2_4:51114970-51114989 None:intergenic 35.0%
! TTATTTCCCCAATTTGGAAC+AGG + chr2_4:51115072-51115091 None:intergenic 35.0%
! GACATCTTATTTCCCCAATT+TGG + chr2_4:51115078-51115097 None:intergenic 35.0%
TTAGTCATCCACAAAGTTGA+TGG + chr2_4:51115300-51115319 None:intergenic 35.0%
!! GAGCTGATTAGTCTTGTTGA+AGG + chr2_4:51113657-51113676 None:intergenic 40.0%
TGAACAAAATCACCATCGGA+TGG + chr2_4:51113711-51113730 None:intergenic 40.0%
! ACTCTAATTGGTGGCATAGA+TGG + chr2_4:51113756-51113775 None:intergenic 40.0%
ATCGGATTCTGCTATGCATA+AGG - chr2_4:51113827-51113846 Msa0333390:CDS 40.0%
CATCTTTGCCTCCAACAATA+GGG - chr2_4:51113937-51113956 Msa0333390:CDS 40.0%
! TTTTCCTCTCTCCCTATTGT+TGG + chr2_4:51113951-51113970 None:intergenic 40.0%
GGAGGCAAGTTGTCATTATT+GGG + chr2_4:51114029-51114048 None:intergenic 40.0%
TGGAGGCAAGTTGTCATTAT+TGG + chr2_4:51114030-51114049 None:intergenic 40.0%
ATAATGACAACTTGCCTCCA+AGG - chr2_4:51114030-51114049 Msa0333390:intron 40.0%
AGTACCTTAGAGGTTAACCT+TGG + chr2_4:51114050-51114069 None:intergenic 40.0%
TCGTTTATGATTAAGCGCGT+AGG - chr2_4:51114123-51114142 Msa0333390:intron 40.0%
ATATGGCAAGTAGTCTGGTA+CGG - chr2_4:51114196-51114215 Msa0333390:intron 40.0%
!! TTTTTAGCCGGTCTACTTCT+TGG + chr2_4:51114769-51114788 None:intergenic 40.0%
!!! AAGAGGGTATTTTGGAGATG+AGG - chr2_4:51114812-51114831 Msa0333390:CDS 40.0%
TGAAGAAGTTCAAGGGTTCA+AGG - chr2_4:51114888-51114907 Msa0333390:CDS 40.0%
AGTTCAAGGGTTCAAGGATT+TGG - chr2_4:51114894-51114913 Msa0333390:CDS 40.0%
GTTCAAGGGTTCAAGGATTT+GGG - chr2_4:51114895-51114914 Msa0333390:CDS 40.0%
AAGAGGACCTTACTTATCAG+AGG - chr2_4:51115017-51115036 Msa0333390:CDS 40.0%
AACCATGCCTCTGATAAGTA+AGG + chr2_4:51115027-51115046 None:intergenic 40.0%
! TTACTTATCAGAGGCATGGT+TGG - chr2_4:51115026-51115045 Msa0333390:CDS 40.0%
AACTCCTCCTGTTCCAAATT+GGG - chr2_4:51115062-51115081 Msa0333390:CDS 40.0%
! CGAAGTATCCATCAACTTTG+TGG - chr2_4:51115289-51115308 Msa0333390:five_prime_UTR 40.0%
! TGGGATCTTTTTGCATCTTG+TGG + chr2_4:51113417-51113436 None:intergenic 40.0%
!! GAGCTGATTAGTCTTGTTGA+AGG + chr2_4:51113657-51113676 None:intergenic 40.0%
TGAACAAAATCACCATCGGA+TGG + chr2_4:51113711-51113730 None:intergenic 40.0%
! ACTCTAATTGGTGGCATAGA+TGG + chr2_4:51113756-51113775 None:intergenic 40.0%
ATCGGATTCTGCTATGCATA+AGG - chr2_4:51113827-51113846 Msa0333390:CDS 40.0%
CATCTTTGCCTCCAACAATA+GGG - chr2_4:51113937-51113956 Msa0333390:CDS 40.0%
! TTTTCCTCTCTCCCTATTGT+TGG + chr2_4:51113951-51113970 None:intergenic 40.0%
GGAGGCAAGTTGTCATTATT+GGG + chr2_4:51114029-51114048 None:intergenic 40.0%
TGGAGGCAAGTTGTCATTAT+TGG + chr2_4:51114030-51114049 None:intergenic 40.0%
ATAATGACAACTTGCCTCCA+AGG - chr2_4:51114030-51114049 Msa0333390:intron 40.0%
AGTACCTTAGAGGTTAACCT+TGG + chr2_4:51114050-51114069 None:intergenic 40.0%
TCGTTTATGATTAAGCGCGT+AGG - chr2_4:51114123-51114142 Msa0333390:intron 40.0%
ATATGGCAAGTAGTCTGGTA+CGG - chr2_4:51114196-51114215 Msa0333390:intron 40.0%
!! TTTTTAGCCGGTCTACTTCT+TGG + chr2_4:51114769-51114788 None:intergenic 40.0%
!!! AAGAGGGTATTTTGGAGATG+AGG - chr2_4:51114812-51114831 Msa0333390:CDS 40.0%
TGAAGAAGTTCAAGGGTTCA+AGG - chr2_4:51114888-51114907 Msa0333390:CDS 40.0%
AGTTCAAGGGTTCAAGGATT+TGG - chr2_4:51114894-51114913 Msa0333390:CDS 40.0%
GTTCAAGGGTTCAAGGATTT+GGG - chr2_4:51114895-51114914 Msa0333390:CDS 40.0%
AAGAGGACCTTACTTATCAG+AGG - chr2_4:51115017-51115036 Msa0333390:CDS 40.0%
AACCATGCCTCTGATAAGTA+AGG + chr2_4:51115027-51115046 None:intergenic 40.0%
! TTACTTATCAGAGGCATGGT+TGG - chr2_4:51115026-51115045 Msa0333390:CDS 40.0%
AACTCCTCCTGTTCCAAATT+GGG - chr2_4:51115062-51115081 Msa0333390:CDS 40.0%
! CGAAGTATCCATCAACTTTG+TGG - chr2_4:51115289-51115308 Msa0333390:five_prime_UTR 40.0%
TCAGCAAGTAAACCATCCGA+TGG - chr2_4:51113696-51113715 Msa0333390:CDS 45.0%
GATTCTGCTATGCATAAGGC+AGG - chr2_4:51113831-51113850 Msa0333390:CDS 45.0%
CCTATTGTTGGAGGCAAAGA+TGG + chr2_4:51113939-51113958 None:intergenic 45.0%
CCATCTTTGCCTCCAACAAT+AGG - chr2_4:51113936-51113955 Msa0333390:CDS 45.0%
!!! AGGTGTCGATGGTTGTTTTC+GGG + chr2_4:51114006-51114025 None:intergenic 45.0%
!!! GAGGTGTCGATGGTTGTTTT+CGG + chr2_4:51114007-51114026 None:intergenic 45.0%
!! TCATTATTGGGAGGTGTCGA+TGG + chr2_4:51114017-51114036 None:intergenic 45.0%
GGCAAGTTGTCATTATTGGG+AGG + chr2_4:51114026-51114045 None:intergenic 45.0%
ACCTTAGAGGTTAACCTTGG+AGG + chr2_4:51114047-51114066 None:intergenic 45.0%
!! TGGTGTTACTTCTTGGTGCT+AGG + chr2_4:51114330-51114349 None:intergenic 45.0%
CAGCATTCCAAGAAGTAGAC+CGG - chr2_4:51114759-51114778 Msa0333390:CDS 45.0%
GAGGAAGATAAAGCAGCAAG+AGG - chr2_4:51115000-51115019 Msa0333390:CDS 45.0%
GACCTTACTTATCAGAGGCA+TGG - chr2_4:51115022-51115041 Msa0333390:CDS 45.0%
CAACTCCTCCTGTTCCAAAT+TGG - chr2_4:51115061-51115080 Msa0333390:CDS 45.0%
ACTCCTCCTGTTCCAAATTG+GGG - chr2_4:51115063-51115082 Msa0333390:CDS 45.0%
! TTTCCCCAATTTGGAACAGG+AGG + chr2_4:51115069-51115088 None:intergenic 45.0%
TCTCCGTCGAAAAACCTAGT+TGG - chr2_4:51115320-51115339 Msa0333390:five_prime_UTR 45.0%
TCAGCAAGTAAACCATCCGA+TGG - chr2_4:51113696-51113715 Msa0333390:CDS 45.0%
GATTCTGCTATGCATAAGGC+AGG - chr2_4:51113831-51113850 Msa0333390:CDS 45.0%
CCTATTGTTGGAGGCAAAGA+TGG + chr2_4:51113939-51113958 None:intergenic 45.0%
CCATCTTTGCCTCCAACAAT+AGG - chr2_4:51113936-51113955 Msa0333390:CDS 45.0%
!!! AGGTGTCGATGGTTGTTTTC+GGG + chr2_4:51114006-51114025 None:intergenic 45.0%
!!! GAGGTGTCGATGGTTGTTTT+CGG + chr2_4:51114007-51114026 None:intergenic 45.0%
!! TCATTATTGGGAGGTGTCGA+TGG + chr2_4:51114017-51114036 None:intergenic 45.0%
GGCAAGTTGTCATTATTGGG+AGG + chr2_4:51114026-51114045 None:intergenic 45.0%
ACCTTAGAGGTTAACCTTGG+AGG + chr2_4:51114047-51114066 None:intergenic 45.0%
!! TGGTGTTACTTCTTGGTGCT+AGG + chr2_4:51114330-51114349 None:intergenic 45.0%
CAGCATTCCAAGAAGTAGAC+CGG - chr2_4:51114759-51114778 Msa0333390:CDS 45.0%
GAGGAAGATAAAGCAGCAAG+AGG - chr2_4:51115000-51115019 Msa0333390:CDS 45.0%
GACCTTACTTATCAGAGGCA+TGG - chr2_4:51115022-51115041 Msa0333390:CDS 45.0%
CAACTCCTCCTGTTCCAAAT+TGG - chr2_4:51115061-51115080 Msa0333390:CDS 45.0%
ACTCCTCCTGTTCCAAATTG+GGG - chr2_4:51115063-51115082 Msa0333390:CDS 45.0%
! TTTCCCCAATTTGGAACAGG+AGG + chr2_4:51115069-51115088 None:intergenic 45.0%
TCTCCGTCGAAAAACCTAGT+TGG - chr2_4:51115320-51115339 Msa0333390:five_prime_UTR 45.0%
GGTGCTCTTTGCAGCTTCTT+AGG + chr2_4:51113735-51113754 None:intergenic 50.0%
TCCTCTCTCCCTATTGTTGG+AGG + chr2_4:51113948-51113967 None:intergenic 50.0%
GCCTCCAAGGTTAACCTCTA+AGG - chr2_4:51114043-51114062 Msa0333390:intron 50.0%
GTCTGGTACGGTTCCAATCA+CGG - chr2_4:51114208-51114227 Msa0333390:intron 50.0%
AACTGCAACGTGACCGTGAT+TGG + chr2_4:51114224-51114243 None:intergenic 50.0%
GGTGCTCTTTGCAGCTTCTT+AGG + chr2_4:51113735-51113754 None:intergenic 50.0%
TCCTCTCTCCCTATTGTTGG+AGG + chr2_4:51113948-51113967 None:intergenic 50.0%
GCCTCCAAGGTTAACCTCTA+AGG - chr2_4:51114043-51114062 Msa0333390:intron 50.0%
GTCTGGTACGGTTCCAATCA+CGG - chr2_4:51114208-51114227 Msa0333390:intron 50.0%
AACTGCAACGTGACCGTGAT+TGG + chr2_4:51114224-51114243 None:intergenic 50.0%
GCCTCCAACAATAGGGAGAG+AGG - chr2_4:51113944-51113963 Msa0333390:CDS 55.0%
CACGGTCACGTTGCAGTTAC+AGG - chr2_4:51114226-51114245 Msa0333390:intron 55.0%
GCCTCCAACAATAGGGAGAG+AGG - chr2_4:51113944-51113963 Msa0333390:CDS 55.0%
CACGGTCACGTTGCAGTTAC+AGG - chr2_4:51114226-51114245 Msa0333390:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 51113415 51115343 51113415 ID=Msa0333390;Name=Msa0333390
chr2_4 mRNA 51113415 51115343 51113415 ID=Msa0333390-mRNA-1;Parent=Msa0333390;Name=Msa0333390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=89|1|1|1|1|1|2|182|322
chr2_4 exon 51113415 51114004 51113415 ID=Msa0333390-mRNA-1:exon:15272;Parent=Msa0333390-mRNA-1
chr2_4 exon 51114694 51115343 51114694 ID=Msa0333390-mRNA-1:exon:15271;Parent=Msa0333390-mRNA-1
chr2_4 five_prime_UTR 51115255 51115343 51115255 ID=Msa0333390-mRNA-1:five_prime_utr;Parent=Msa0333390-mRNA-1
chr2_4 CDS 51114694 51115254 51114694 ID=Msa0333390-mRNA-1:cds;Parent=Msa0333390-mRNA-1
chr2_4 CDS 51113597 51114004 51113597 ID=Msa0333390-mRNA-1:cds;Parent=Msa0333390-mRNA-1
chr2_4 three_prime_UTR 51113415 51113596 51113415 ID=Msa0333390-mRNA-1:three_prime_utr;Parent=Msa0333390-mRNA-1
Gene Sequence

>Msa0333390

ATGGATCCAAATGATAATAATAGCTCTTGTGAATCTTCTTTTTGCAAAGATGAAAAAGTGGAAGCAATGAATCTACTTGAAGAGTGTTGGTATTTTGATAACTTGTTAAATATAAAACCAAAGATGTTAAGATGTCATTCTGATCCTTACCCTTCAACAAGACTAATCAGCTCAGAACTTTTGAAGGATTCATCAGCAAGTAAACCATCCGATGGTGATTTTGTTCATCCTAAGAAGCTGCAAAGAGCACCATCTATGCCACCAATTAGAGTGAAAGAAGAAGAAAAAGATGATAAAAAAGGAAGCAAATTGGTGCATCAAACATCGGATTCTGCTATGCATAAGGCAGGTAGTAAACATCATTGTGCAAAGATGAAAGGACTTGATCATAGAAGTGATAGCAATAGAAGGAAAAGCAAATTGCTAAGAACACCATCTTTGCCTCCAACAATAGGGAGAGAGGAAAAGTTTCAAGAAACTGATTCAAGAATTGGAAGATCCCGAAAACAACCATCGACACCTCCCAATAATGACAACTTGCCTCCAAGGTTAACCTCTAAGAGTTGCAGCATTCCAAGAAGTAGACCGGCTAAAAACATCGAAGTTGAAAGCTTAAACAAAGAGGGTATTTTGGAGATGAGGAGAAAGGTCCTAAATCAGAAAACAATAAGGAGAAGCCTAAGTGATCTTGAGTATGAAGAAGTTCAAGGGTTCAAGGATTTGGGATTCTCATTTGAGAAAGAAGCTTTAAGTCCAAGCTTAGCTAACATAATTCCTGGTTTGCAAGAGAAAAATAGAGATGAAAGTGAGGAAGATAAAGCAGCAAGAGGACCTTACTTATCAGAGGCATGGTTGGTGCAAAGTTGTGCAACTCCTCCTGTTCCAAATTGGGGAAATAAGATGTCTGCAGATGACATGAAAAAACATATCAAGTTTTGGGCTAGAGCTGTTGCTTCAACTAAATGTTGA
Protein sequence

>Msa0333390

MDPNDNNSSCESSFCKDEKVEAMNLLEECWYFDNLLNIKPKMLRCHSDPYPSTRLISSELLKDSSASKPSDGDFVHPKKLQRAPSMPPIRVKEEEKDDKKGSKLVHQTSDSAMHKAGSKHHCAKMKGLDHRSDSNRRKSKLLRTPSLPPTIGREEKFQETDSRIGRSRKQPSTPPNNDNLPPRLTSKSCSIPRSRPAKNIEVESLNKEGILEMRRKVLNQKTIRRSLSDLEYEEVQGFKDLGFSFEKEALSPSLANIIPGLQEKNRDESEEDKAARGPYLSEAWLVQSCATPPVPNWGNKMSADDMKKHIKFWARAVASTKC*