AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0217380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217380 MtrunA17_Chr2g0319191 94.697 132 7 0 1 132 1 132 5.14e-86 246
Msa0217380 MtrunA17_Chr2g0319181 94.697 132 6 1 1 132 1 131 5.44e-85 244
Msa0217380 MtrunA17_Chr7g0268831 85.600 125 14 1 3 127 13 133 2.89e-70 207
Msa0217380 MtrunA17_Chr7g0273351 85.600 125 14 1 3 127 10 130 3.16e-70 207
Msa0217380 MtrunA17_Chr7g0273331 85.600 125 14 1 3 127 66 186 2.19e-69 207
Msa0217380 MtrunA17_Chr7g0259151 79.200 125 22 1 3 127 13 133 1.09e-67 201
Msa0217380 MtrunA17_Chr7g0272611 78.378 111 24 0 11 121 21 131 1.70e-58 177
Msa0217380 MtrunA17_Chr2g0327951 78.704 108 23 0 14 121 57 164 2.96e-58 178
Msa0217380 MtrunA17_Chr4g0031041 77.982 109 24 0 14 122 16 124 1.68e-57 175
Msa0217380 MtrunA17_Chr4g0031001 77.570 107 24 0 16 122 24 130 2.03e-56 172
Msa0217380 MtrunA17_Chr4g0031651 77.570 107 24 0 16 122 24 130 2.07e-56 172
Msa0217380 MtrunA17_Chr4g0035621 77.570 107 24 0 16 122 24 130 3.41e-56 172
Msa0217380 MtrunA17_Chr7g0273341 80.508 118 10 3 13 130 55 159 3.78e-56 172
Msa0217380 MtrunA17_Chr4g0032301 78.302 106 23 0 16 121 24 129 3.82e-56 172
Msa0217380 MtrunA17_Chr8g0379311 77.570 107 24 0 16 122 24 130 4.07e-56 172
Msa0217380 MtrunA17_Chr2g0327931 77.570 107 24 0 16 122 23 129 4.43e-56 172
Msa0217380 MtrunA17_Chr1g0148961 64.545 110 37 2 14 122 24 132 1.58e-40 132
Msa0217380 MtrunA17_Chr1g0148951 64.545 110 37 2 14 122 24 132 1.58e-40 132
Msa0217380 MtrunA17_Chr6g0449661 63.303 109 31 2 16 123 85 185 2.34e-40 133
Msa0217380 MtrunA17_Chr3g0119971 62.037 108 32 2 17 123 81 180 1.83e-39 131
Msa0217380 MtrunA17_Chr1g0211651 58.462 130 45 4 1 122 1 129 2.79e-39 128
Msa0217380 MtrunA17_Chr1g0184901 64.486 107 36 2 17 122 27 132 1.04e-38 127
Msa0217380 MtrunA17_Chr4g0031031 78.378 74 16 0 37 110 10 83 5.08e-35 117
Msa0217380 MtrunA17_Chr4g0000531 85.714 56 8 0 77 132 5 60 7.27e-29 100
Msa0217380 MtrunA17_Chr4g0024701 51.429 105 31 2 16 120 26 110 2.07e-25 92.8
Msa0217380 MtrunA17_Chr1g0183701 50.685 73 36 0 59 131 30 102 1.96e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217380 AT1G51060.1 93.939 132 8 0 1 132 1 132 5.00e-86 247
Msa0217380 AT4G27230.2 95.420 131 6 0 1 131 1 131 8.84e-86 246
Msa0217380 AT4G27230.1 95.420 131 6 0 1 131 1 131 8.84e-86 246
Msa0217380 AT5G54640.1 98.413 126 2 0 1 126 1 126 7.59e-85 244
Msa0217380 AT3G20670.1 91.667 132 11 0 1 132 1 132 2.30e-83 240
Msa0217380 AT1G54690.1 84.496 129 17 1 2 127 5 133 8.70e-71 209
Msa0217380 AT1G08880.1 83.721 129 18 1 2 127 5 133 1.87e-70 208
Msa0217380 AT5G59870.1 76.852 108 25 0 15 122 23 130 1.10e-57 176
Msa0217380 AT5G27670.1 73.333 120 32 0 11 130 20 139 6.44e-57 174
Msa0217380 AT5G02560.1 77.064 109 25 0 14 122 22 130 9.21e-57 174
Msa0217380 AT5G02560.2 63.158 133 25 1 14 122 22 154 2.77e-51 160
Msa0217380 AT2G38810.1 62.963 108 38 2 14 120 26 132 5.44e-38 125
Msa0217380 AT2G38810.4 62.963 108 38 2 14 120 26 132 5.44e-38 125
Msa0217380 AT2G38810.3 62.963 108 38 2 14 120 26 132 5.44e-38 125
Msa0217380 AT2G38810.2 62.963 108 38 2 14 120 26 132 5.44e-38 125
Msa0217380 AT1G52740.1 63.208 106 37 2 18 122 28 132 7.27e-38 125
Msa0217380 AT3G54560.1 62.500 104 37 2 18 120 30 132 2.86e-36 121
Msa0217380 AT3G54560.2 62.500 104 37 2 18 120 30 132 2.86e-36 121
Msa0217380 AT4G13570.2 44.037 109 59 2 7 114 17 124 2.24e-22 85.9
Msa0217380 AT4G13570.1 50.000 92 44 2 24 114 28 118 5.36e-22 84.7

Find 50 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAACAAGTTGTGAATATT+TGG 0.203212 2_1:-64245611 None:intergenic
TTGGAAGGACCAGCTTTCTT+TGG 0.296401 2_1:-64245635 None:intergenic
GAAAGATTGAAATACTAAAA+AGG 0.335968 2_1:-64245833 None:intergenic
TTCAATCTTTCGCAGTTCTT+TGG 0.343247 2_1:+64245845 Msa0217380:three_prime_UTR
AAAATTTAATTGTGTTAAAT+GGG 0.358658 2_1:+64245702 Msa0217380:three_prime_UTR
GTCGTTGTAGTTCTAGTTAG+TGG 0.363313 2_1:+64245730 Msa0217380:three_prime_UTR
GAAGAATTGAGCAAGCTTCT+TGG 0.376859 2_1:+64245557 Msa0217380:CDS
AGTACGGCGGCGAGGTAAAC+AGG 0.402491 2_1:-64245220 None:intergenic
TATTTCAATCTCTTCATCAA+TGG 0.413109 2_1:+64245054 Msa0217380:exon
GGTGATGTTACCATTGCTAA+TGG 0.441296 2_1:+64245578 Msa0217380:CDS
TAATTGTGTTAAATGGGTCT+TGG 0.444820 2_1:+64245708 Msa0217380:three_prime_UTR
TTTATCTGTTATTATCATCA+TGG 0.446226 2_1:+64245908 Msa0217380:three_prime_UTR
TTCATCTTCAGCAGCACCCT+TGG 0.446246 2_1:-64245654 None:intergenic
ACCTCAAGAAGCAGCAAAGC+TGG 0.455395 2_1:+64245121 Msa0217380:CDS
AGCTTTGCTGCTTCTTGAGG+TGG 0.455549 2_1:-64245119 None:intergenic
TGTGCCAAGGCATATTCAAT+TGG 0.459542 2_1:+64245520 Msa0217380:CDS
ACCAGCTTTGCTGCTTCTTG+AGG 0.463693 2_1:-64245122 None:intergenic
AGAGGCAAAACCCTTGGTTC+CGG 0.465510 2_1:+64245082 Msa0217380:CDS
TGGATATATTTATCAAAGCT+TGG 0.483607 2_1:+64245773 Msa0217380:three_prime_UTR
AGAGAGAGAGATTTGAAGAA+CGG 0.495986 2_1:-64245017 None:intergenic
TTTAGTTAATGATGTTTATG+TGG 0.504931 2_1:+64245753 Msa0217380:three_prime_UTR
TTGTTGCTTCCAAAGAAAGC+TGG 0.506478 2_1:+64245626 Msa0217380:CDS
TCAATCTCTTCATCAATGGC+TGG 0.508829 2_1:+64245058 Msa0217380:exon
AAAGAAAGCTGGTCCTTCCA+AGG 0.525187 2_1:+64245637 Msa0217380:CDS
ATCGCAAGATTCCTCAAGGC+CGG 0.538535 2_1:+64245166 Msa0217380:CDS
TCTTCATCAATGGCTGGTAG+AGG 0.543571 2_1:+64245064 Msa0217380:exon
GGAAAATACGCTGAACGTGT+TGG 0.559261 2_1:+64245187 Msa0217380:CDS
CTTAGCAGCACCGGAACCAA+GGG 0.561448 2_1:-64245092 None:intergenic
TACGCTGAACGTGTTGGTGC+TGG 0.564093 2_1:+64245193 Msa0217380:CDS
TCTTAGCAGCACCGGAACCA+AGG 0.565384 2_1:-64245093 None:intergenic
TCTTCAGCAGCACCCTTGGA+AGG 0.566645 2_1:-64245650 None:intergenic
GCTGGTAGAGGCAAAACCCT+TGG 0.567239 2_1:+64245076 Msa0217380:CDS
GAATCTTGCGATTCTTCCGA+CGG 0.571187 2_1:-64245155 None:intergenic
GAGAGATTTGAAGAACGGTG+AGG 0.576871 2_1:-64245012 None:intergenic
ACAGCCAATTGAATATGCCT+TGG 0.577584 2_1:-64245524 None:intergenic
AAGAAAGCTGGTCCTTCCAA+GGG 0.587991 2_1:+64245638 Msa0217380:CDS
GGTGGCTTTCTTAGCAGCAC+CGG 0.590996 2_1:-64245101 None:intergenic
AATCTTGCGATTCTTCCGAC+GGG 0.601933 2_1:-64245154 None:intergenic
AAGAATCGCAAGATTCCTCA+AGG 0.619754 2_1:+64245162 Msa0217380:CDS
GTTGGCTGGAAATGCTGCAA+GGG 0.629600 2_1:+64245478 Msa0217380:CDS
AGCGGCGAGATATTCGAGTA+CGG 0.641335 2_1:-64245236 None:intergenic
CATAACACCACCATTAGCAA+TGG 0.644464 2_1:-64245588 None:intergenic
ACTCGAATATCTCGCCGCTG+AGG 0.650436 2_1:+64245240 Msa0217380:CDS
AGAAGACAAGAATTGTGCCA+AGG 0.658370 2_1:+64245507 Msa0217380:CDS
GCTGGTCTTCAATTTCCCGT+CGG 0.659854 2_1:+64245139 Msa0217380:CDS
AGTTGGCTGGAAATGCTGCA+AGG 0.665089 2_1:+64245477 Msa0217380:CDS
GATGTTACCATTGCTAATGG+TGG 0.667704 2_1:+64245581 Msa0217380:CDS
GATATTCGAGTACGGCGGCG+AGG 0.699553 2_1:-64245228 None:intergenic
GGTGATGGAAATTACCTCAG+CGG 0.723800 2_1:-64245254 None:intergenic
GGCGAGATATTCGAGTACGG+CGG 0.753133 2_1:-64245233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGAATAATTACTAAAAT+TGG - chr2_1:64245349-64245368 None:intergenic 10.0%
!! AAAAATTTAATTGTGTTAAA+TGG + chr2_1:64245701-64245720 Msa0217380:three_prime_UTR 10.0%
!! AAAATTTAATTGTGTTAAAT+GGG + chr2_1:64245702-64245721 Msa0217380:three_prime_UTR 10.0%
!! TTATGAATAATTACTAAAAT+TGG - chr2_1:64245349-64245368 None:intergenic 10.0%
!! AAAAATTTAATTGTGTTAAA+TGG + chr2_1:64245701-64245720 Msa0217380:three_prime_UTR 10.0%
!! AAAATTTAATTGTGTTAAAT+GGG + chr2_1:64245702-64245721 Msa0217380:three_prime_UTR 10.0%
!!! AATTTGTGATTTTTGTGTTT+AGG + chr2_1:64245448-64245467 Msa0217380:intron 20.0%
!! TTTAGTTAATGATGTTTATG+TGG + chr2_1:64245753-64245772 Msa0217380:three_prime_UTR 20.0%
!!! TTTTCATTAATCTCTTCAAA+AGG - chr2_1:64245800-64245819 None:intergenic 20.0%
!! GAAAGATTGAAATACTAAAA+AGG - chr2_1:64245836-64245855 None:intergenic 20.0%
!!! AATTTGTGATTTTTGTGTTT+AGG + chr2_1:64245448-64245467 Msa0217380:intron 20.0%
!! TTTAGTTAATGATGTTTATG+TGG + chr2_1:64245753-64245772 Msa0217380:three_prime_UTR 20.0%
!!! TTTTCATTAATCTCTTCAAA+AGG - chr2_1:64245800-64245819 None:intergenic 20.0%
!! GAAAGATTGAAATACTAAAA+AGG - chr2_1:64245836-64245855 None:intergenic 20.0%
! TATTTCAATCTCTTCATCAA+TGG + chr2_1:64245054-64245073 Msa0217380:exon 25.0%
! AGGGTTTCTTAAATTCAATT+AGG + chr2_1:64245317-64245336 Msa0217380:intron 25.0%
!! TTGTTGAATTTGAAGTGATT+TGG + chr2_1:64245404-64245423 Msa0217380:intron 25.0%
!! TGTTGAATTTGAAGTGATTT+GGG + chr2_1:64245405-64245424 Msa0217380:intron 25.0%
!!! TGATTTTTGTGTTTAGGTTT+TGG + chr2_1:64245454-64245473 Msa0217380:intron 25.0%
! TGGATATATTTATCAAAGCT+TGG + chr2_1:64245773-64245792 Msa0217380:three_prime_UTR 25.0%
! TATTTCAATCTCTTCATCAA+TGG + chr2_1:64245054-64245073 Msa0217380:exon 25.0%
! AGGGTTTCTTAAATTCAATT+AGG + chr2_1:64245317-64245336 Msa0217380:intron 25.0%
!! TTGTTGAATTTGAAGTGATT+TGG + chr2_1:64245404-64245423 Msa0217380:intron 25.0%
!! TGTTGAATTTGAAGTGATTT+GGG + chr2_1:64245405-64245424 Msa0217380:intron 25.0%
!!! TGATTTTTGTGTTTAGGTTT+TGG + chr2_1:64245454-64245473 Msa0217380:intron 25.0%
! TGGATATATTTATCAAAGCT+TGG + chr2_1:64245773-64245792 Msa0217380:three_prime_UTR 25.0%
AATTTAAGAAACCCTAGATC+TGG - chr2_1:64245312-64245331 None:intergenic 30.0%
AGCAACAAGTTGTGAATATT+TGG - chr2_1:64245614-64245633 None:intergenic 30.0%
TAATTGTGTTAAATGGGTCT+TGG + chr2_1:64245708-64245727 Msa0217380:three_prime_UTR 30.0%
AATTTAAGAAACCCTAGATC+TGG - chr2_1:64245312-64245331 None:intergenic 30.0%
AGCAACAAGTTGTGAATATT+TGG - chr2_1:64245614-64245633 None:intergenic 30.0%
TAATTGTGTTAAATGGGTCT+TGG + chr2_1:64245708-64245727 Msa0217380:three_prime_UTR 30.0%
AGAGAGAGAGATTTGAAGAA+CGG - chr2_1:64245020-64245039 None:intergenic 35.0%
ATATTGAAGGTGAAAGAAGG+TGG - chr2_1:64245278-64245297 None:intergenic 35.0%
GTGATATTGAAGGTGAAAGA+AGG - chr2_1:64245281-64245300 None:intergenic 35.0%
GGTTATGTGTGTGATATTGA+AGG - chr2_1:64245291-64245310 None:intergenic 35.0%
!! TGAAGTGATTTGGGTTGATT+AGG + chr2_1:64245414-64245433 Msa0217380:intron 35.0%
!! GAAGTGATTTGGGTTGATTA+GGG + chr2_1:64245415-64245434 Msa0217380:intron 35.0%
!!! TTGTGTTTAGGTTTTGGAGT+TGG + chr2_1:64245460-64245479 Msa0217380:intron 35.0%
TTCAATCTTTCGCAGTTCTT+TGG + chr2_1:64245845-64245864 Msa0217380:three_prime_UTR 35.0%
AGAGAGAGAGATTTGAAGAA+CGG - chr2_1:64245020-64245039 None:intergenic 35.0%
ATATTGAAGGTGAAAGAAGG+TGG - chr2_1:64245278-64245297 None:intergenic 35.0%
GTGATATTGAAGGTGAAAGA+AGG - chr2_1:64245281-64245300 None:intergenic 35.0%
GGTTATGTGTGTGATATTGA+AGG - chr2_1:64245291-64245310 None:intergenic 35.0%
!! TGAAGTGATTTGGGTTGATT+AGG + chr2_1:64245414-64245433 Msa0217380:intron 35.0%
!! GAAGTGATTTGGGTTGATTA+GGG + chr2_1:64245415-64245434 Msa0217380:intron 35.0%
!!! TTGTGTTTAGGTTTTGGAGT+TGG + chr2_1:64245460-64245479 Msa0217380:intron 35.0%
TTCAATCTTTCGCAGTTCTT+TGG + chr2_1:64245845-64245864 Msa0217380:three_prime_UTR 35.0%
! GAACGGTGAGGTTTTTGAAT+CGG - chr2_1:64245003-64245022 None:intergenic 40.0%
TCAATCTCTTCATCAATGGC+TGG + chr2_1:64245058-64245077 Msa0217380:exon 40.0%
AAGAATCGCAAGATTCCTCA+AGG + chr2_1:64245162-64245181 Msa0217380:CDS 40.0%
! AACACGTTCAGCGTATTTTC+CGG - chr2_1:64245188-64245207 None:intergenic 40.0%
TCACACACATAACCAGATCT+AGG + chr2_1:64245297-64245316 Msa0217380:intron 40.0%
CACACACATAACCAGATCTA+GGG + chr2_1:64245298-64245317 Msa0217380:intron 40.0%
AGAAGACAAGAATTGTGCCA+AGG + chr2_1:64245507-64245526 Msa0217380:CDS 40.0%
TGTGCCAAGGCATATTCAAT+TGG + chr2_1:64245520-64245539 Msa0217380:CDS 40.0%
ACAGCCAATTGAATATGCCT+TGG - chr2_1:64245527-64245546 None:intergenic 40.0%
GAAGAATTGAGCAAGCTTCT+TGG + chr2_1:64245557-64245576 Msa0217380:CDS 40.0%
GGTGATGTTACCATTGCTAA+TGG + chr2_1:64245578-64245597 Msa0217380:CDS 40.0%
GATGTTACCATTGCTAATGG+TGG + chr2_1:64245581-64245600 Msa0217380:CDS 40.0%
CATAACACCACCATTAGCAA+TGG - chr2_1:64245591-64245610 None:intergenic 40.0%
! TTGTTGCTTCCAAAGAAAGC+TGG + chr2_1:64245626-64245645 Msa0217380:CDS 40.0%
!! GTCGTTGTAGTTCTAGTTAG+TGG + chr2_1:64245730-64245749 Msa0217380:three_prime_UTR 40.0%
! GAACGGTGAGGTTTTTGAAT+CGG - chr2_1:64245003-64245022 None:intergenic 40.0%
TCAATCTCTTCATCAATGGC+TGG + chr2_1:64245058-64245077 Msa0217380:exon 40.0%
AAGAATCGCAAGATTCCTCA+AGG + chr2_1:64245162-64245181 Msa0217380:CDS 40.0%
! AACACGTTCAGCGTATTTTC+CGG - chr2_1:64245188-64245207 None:intergenic 40.0%
TCACACACATAACCAGATCT+AGG + chr2_1:64245297-64245316 Msa0217380:intron 40.0%
CACACACATAACCAGATCTA+GGG + chr2_1:64245298-64245317 Msa0217380:intron 40.0%
AGAAGACAAGAATTGTGCCA+AGG + chr2_1:64245507-64245526 Msa0217380:CDS 40.0%
TGTGCCAAGGCATATTCAAT+TGG + chr2_1:64245520-64245539 Msa0217380:CDS 40.0%
ACAGCCAATTGAATATGCCT+TGG - chr2_1:64245527-64245546 None:intergenic 40.0%
GAAGAATTGAGCAAGCTTCT+TGG + chr2_1:64245557-64245576 Msa0217380:CDS 40.0%
GGTGATGTTACCATTGCTAA+TGG + chr2_1:64245578-64245597 Msa0217380:CDS 40.0%
GATGTTACCATTGCTAATGG+TGG + chr2_1:64245581-64245600 Msa0217380:CDS 40.0%
CATAACACCACCATTAGCAA+TGG - chr2_1:64245591-64245610 None:intergenic 40.0%
! TTGTTGCTTCCAAAGAAAGC+TGG + chr2_1:64245626-64245645 Msa0217380:CDS 40.0%
!! GTCGTTGTAGTTCTAGTTAG+TGG + chr2_1:64245730-64245749 Msa0217380:three_prime_UTR 40.0%
GAGAGATTTGAAGAACGGTG+AGG - chr2_1:64245015-64245034 None:intergenic 45.0%
TCTTCATCAATGGCTGGTAG+AGG + chr2_1:64245064-64245083 Msa0217380:exon 45.0%
AATCTTGCGATTCTTCCGAC+GGG - chr2_1:64245157-64245176 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG - chr2_1:64245158-64245177 None:intergenic 45.0%
GGAAAATACGCTGAACGTGT+TGG + chr2_1:64245187-64245206 Msa0217380:CDS 45.0%
!! GGTGATGGAAATTACCTCAG+CGG - chr2_1:64245257-64245276 None:intergenic 45.0%
AAGGTGAAAGAAGGTGGTGA+TGG - chr2_1:64245272-64245291 None:intergenic 45.0%
!!! GTTTAGGTTTTGGAGTTGGC+TGG + chr2_1:64245464-64245483 Msa0217380:intron 45.0%
TTGGAAGGACCAGCTTTCTT+TGG - chr2_1:64245638-64245657 None:intergenic 45.0%
AAAGAAAGCTGGTCCTTCCA+AGG + chr2_1:64245637-64245656 Msa0217380:CDS 45.0%
AAGAAAGCTGGTCCTTCCAA+GGG + chr2_1:64245638-64245657 Msa0217380:CDS 45.0%
GAGAGATTTGAAGAACGGTG+AGG - chr2_1:64245015-64245034 None:intergenic 45.0%
TCTTCATCAATGGCTGGTAG+AGG + chr2_1:64245064-64245083 Msa0217380:exon 45.0%
AATCTTGCGATTCTTCCGAC+GGG - chr2_1:64245157-64245176 None:intergenic 45.0%
GAATCTTGCGATTCTTCCGA+CGG - chr2_1:64245158-64245177 None:intergenic 45.0%
GGAAAATACGCTGAACGTGT+TGG + chr2_1:64245187-64245206 Msa0217380:CDS 45.0%
!! GGTGATGGAAATTACCTCAG+CGG - chr2_1:64245257-64245276 None:intergenic 45.0%
AAGGTGAAAGAAGGTGGTGA+TGG - chr2_1:64245272-64245291 None:intergenic 45.0%
!!! GTTTAGGTTTTGGAGTTGGC+TGG + chr2_1:64245464-64245483 Msa0217380:intron 45.0%
TTGGAAGGACCAGCTTTCTT+TGG - chr2_1:64245638-64245657 None:intergenic 45.0%
AAAGAAAGCTGGTCCTTCCA+AGG + chr2_1:64245637-64245656 Msa0217380:CDS 45.0%
AAGAAAGCTGGTCCTTCCAA+GGG + chr2_1:64245638-64245657 Msa0217380:CDS 45.0%
AGAGGCAAAACCCTTGGTTC+CGG + chr2_1:64245082-64245101 Msa0217380:CDS 50.0%
! AGCTTTGCTGCTTCTTGAGG+TGG - chr2_1:64245122-64245141 None:intergenic 50.0%
ACCTCAAGAAGCAGCAAAGC+TGG + chr2_1:64245121-64245140 Msa0217380:CDS 50.0%
! ACCAGCTTTGCTGCTTCTTG+AGG - chr2_1:64245125-64245144 None:intergenic 50.0%
!! GCTGGTCTTCAATTTCCCGT+CGG + chr2_1:64245139-64245158 Msa0217380:CDS 50.0%
ATCGCAAGATTCCTCAAGGC+CGG + chr2_1:64245166-64245185 Msa0217380:CDS 50.0%
AGCGGCGAGATATTCGAGTA+CGG - chr2_1:64245239-64245258 None:intergenic 50.0%
AGTTGGCTGGAAATGCTGCA+AGG + chr2_1:64245477-64245496 Msa0217380:CDS 50.0%
GTTGGCTGGAAATGCTGCAA+GGG + chr2_1:64245478-64245497 Msa0217380:CDS 50.0%
TTCATCTTCAGCAGCACCCT+TGG - chr2_1:64245657-64245676 None:intergenic 50.0%
AGAGGCAAAACCCTTGGTTC+CGG + chr2_1:64245082-64245101 Msa0217380:CDS 50.0%
! AGCTTTGCTGCTTCTTGAGG+TGG - chr2_1:64245122-64245141 None:intergenic 50.0%
ACCTCAAGAAGCAGCAAAGC+TGG + chr2_1:64245121-64245140 Msa0217380:CDS 50.0%
! ACCAGCTTTGCTGCTTCTTG+AGG - chr2_1:64245125-64245144 None:intergenic 50.0%
!! GCTGGTCTTCAATTTCCCGT+CGG + chr2_1:64245139-64245158 Msa0217380:CDS 50.0%
ATCGCAAGATTCCTCAAGGC+CGG + chr2_1:64245166-64245185 Msa0217380:CDS 50.0%
AGCGGCGAGATATTCGAGTA+CGG - chr2_1:64245239-64245258 None:intergenic 50.0%
AGTTGGCTGGAAATGCTGCA+AGG + chr2_1:64245477-64245496 Msa0217380:CDS 50.0%
GTTGGCTGGAAATGCTGCAA+GGG + chr2_1:64245478-64245497 Msa0217380:CDS 50.0%
TTCATCTTCAGCAGCACCCT+TGG - chr2_1:64245657-64245676 None:intergenic 50.0%
GCTGGTAGAGGCAAAACCCT+TGG + chr2_1:64245076-64245095 Msa0217380:CDS 55.0%
! CTTAGCAGCACCGGAACCAA+GGG - chr2_1:64245095-64245114 None:intergenic 55.0%
! TCTTAGCAGCACCGGAACCA+AGG - chr2_1:64245096-64245115 None:intergenic 55.0%
GGTGGCTTTCTTAGCAGCAC+CGG - chr2_1:64245104-64245123 None:intergenic 55.0%
! CAGCGTATTTTCCGGCCTTG+AGG - chr2_1:64245180-64245199 None:intergenic 55.0%
! TACGCTGAACGTGTTGGTGC+TGG + chr2_1:64245193-64245212 Msa0217380:CDS 55.0%
GGCGAGATATTCGAGTACGG+CGG - chr2_1:64245236-64245255 None:intergenic 55.0%
ACTCGAATATCTCGCCGCTG+AGG + chr2_1:64245240-64245259 Msa0217380:CDS 55.0%
TCTTCAGCAGCACCCTTGGA+AGG - chr2_1:64245653-64245672 None:intergenic 55.0%
GCTGGTAGAGGCAAAACCCT+TGG + chr2_1:64245076-64245095 Msa0217380:CDS 55.0%
! CTTAGCAGCACCGGAACCAA+GGG - chr2_1:64245095-64245114 None:intergenic 55.0%
! TCTTAGCAGCACCGGAACCA+AGG - chr2_1:64245096-64245115 None:intergenic 55.0%
GGTGGCTTTCTTAGCAGCAC+CGG - chr2_1:64245104-64245123 None:intergenic 55.0%
! CAGCGTATTTTCCGGCCTTG+AGG - chr2_1:64245180-64245199 None:intergenic 55.0%
! TACGCTGAACGTGTTGGTGC+TGG + chr2_1:64245193-64245212 Msa0217380:CDS 55.0%
GGCGAGATATTCGAGTACGG+CGG - chr2_1:64245236-64245255 None:intergenic 55.0%
ACTCGAATATCTCGCCGCTG+AGG + chr2_1:64245240-64245259 Msa0217380:CDS 55.0%
TCTTCAGCAGCACCCTTGGA+AGG - chr2_1:64245653-64245672 None:intergenic 55.0%
AGTACGGCGGCGAGGTAAAC+AGG - chr2_1:64245223-64245242 None:intergenic 60.0%
GATATTCGAGTACGGCGGCG+AGG - chr2_1:64245231-64245250 None:intergenic 60.0%
AGTACGGCGGCGAGGTAAAC+AGG - chr2_1:64245223-64245242 None:intergenic 60.0%
GATATTCGAGTACGGCGGCG+AGG - chr2_1:64245231-64245250 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 64244968 64245929 64244968 ID=Msa0217380;Name=Msa0217380
chr2_1 mRNA 64244968 64245929 64244968 ID=Msa0217380-mRNA-1;Parent=Msa0217380;Name=Msa0217380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=105|1|1|1|1|1|2|250|132
chr2_1 exon 64244968 64245261 64244968 ID=Msa0217380-mRNA-1:exon:17771;Parent=Msa0217380-mRNA-1
chr2_1 exon 64245470 64245929 64245470 ID=Msa0217380-mRNA-1:exon:17772;Parent=Msa0217380-mRNA-1
chr2_1 five_prime_UTR 64244968 64245072 64244968 ID=Msa0217380-mRNA-1:five_prime_utr;Parent=Msa0217380-mRNA-1
chr2_1 CDS 64245073 64245261 64245073 ID=Msa0217380-mRNA-1:cds;Parent=Msa0217380-mRNA-1
chr2_1 CDS 64245470 64245679 64245470 ID=Msa0217380-mRNA-1:cds;Parent=Msa0217380-mRNA-1
chr2_1 three_prime_UTR 64245680 64245929 64245680 ID=Msa0217380-mRNA-1:three_prime_utr;Parent=Msa0217380-mRNA-1
Gene Sequence

>Msa0217380

ATGGCTGGTAGAGGCAAAACCCTTGGTTCCGGTGCTGCTAAGAAAGCCACCTCAAGAAGCAGCAAAGCTGGTCTTCAATTTCCCGTCGGAAGAATCGCAAGATTCCTCAAGGCCGGAAAATACGCTGAACGTGTTGGTGCTGGTGCTCCTGTTTACCTCGCCGCCGTACTCGAATATCTCGCCGCTGAGGTTTTGGAGTTGGCTGGAAATGCTGCAAGGGACAACAAGAAGACAAGAATTGTGCCAAGGCATATTCAATTGGCTGTGAGAAATGATGAAGAATTGAGCAAGCTTCTTGGTGATGTTACCATTGCTAATGGTGGTGTTATGCCAAATATTCACAACTTGTTGCTTCCAAAGAAAGCTGGTCCTTCCAAGGGTGCTGCTGAAGATGAATGA
Protein sequence

>Msa0217380

MAGRGKTLGSGAAKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAVLEYLAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLLPKKAGPSKGAAEDE*