AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0218100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218100 MtrunA17_Chr2g0320251 96.056 431 13 1 1 431 17 443 0.0 862
Msa0218100 MtrunA17_Chr4g0028201 73.708 445 101 3 1 431 19 461 0.0 654
Msa0218100 MtrunA17_Chr8g0385651 44.751 362 192 5 58 418 34 388 1.62e-104 316
Msa0218100 MtrunA17_Chr8g0385671 42.894 387 210 6 33 418 12 388 3.28e-104 315
Msa0218100 MtrunA17_Chr1g0179651 30.088 339 185 11 65 386 1 304 2.80e-37 139
Msa0218100 MtrunA17_Chr7g0265461 27.528 356 227 8 64 414 60 389 1.85e-34 132
Msa0218100 MtrunA17_Chr7g0265451 30.539 334 195 10 62 386 175 480 9.17e-34 132
Msa0218100 MtrunA17_Chr1g0173651 28.224 411 211 14 65 404 342 739 1.20e-31 127
Msa0218100 MtrunA17_Chr5g0411991 27.654 358 210 12 69 414 86 406 4.31e-30 120
Msa0218100 MtrunA17_Chr4g0055991 31.399 293 172 10 120 398 244 521 1.03e-28 118
Msa0218100 MtrunA17_Chr3g0131051 26.606 327 217 6 62 386 151 456 3.08e-28 116
Msa0218100 MtrunA17_Chr4g0064471 25.138 362 220 12 71 414 133 461 1.47e-26 111
Msa0218100 MtrunA17_Chr4g0039811 25.587 426 241 15 36 411 61 460 1.18e-25 108
Msa0218100 MtrunA17_Chr7g0253441 26.361 349 221 9 72 414 154 472 6.48e-25 106
Msa0218100 MtrunA17_Chr7g0265381 27.273 352 216 11 73 414 44 365 1.47e-24 104
Msa0218100 MtrunA17_Chr4g0064411 25.648 386 245 12 40 414 99 453 1.58e-24 105
Msa0218100 MtrunA17_Chr3g0137491 26.891 357 217 13 71 414 57 382 1.64e-24 104
Msa0218100 MtrunA17_Chr4g0064461 26.316 361 215 14 72 414 129 456 1.12e-22 100
Msa0218100 MtrunA17_Chr3g0120131 28.443 334 191 15 68 386 147 447 1.73e-22 99.8
Msa0218100 MtrunA17_Chr3g0119721 25.641 351 222 14 73 414 15 335 2.39e-22 97.4
Msa0218100 MtrunA17_Chr3g0140131 25.926 351 222 10 73 414 264 585 7.21e-22 98.2
Msa0218100 MtrunA17_Chr8g0390121 27.437 277 157 8 122 383 325 572 5.61e-20 92.4
Msa0218100 MtrunA17_Chr1g0197381 26.441 295 192 9 122 414 268 539 7.97e-19 89.0
Msa0218100 MtrunA17_Chr1g0146181 25.938 320 177 11 73 371 257 537 6.38e-18 86.3
Msa0218100 MtrunA17_Chr6g0460631 24.826 431 259 15 38 426 77 484 6.85e-18 85.9
Msa0218100 MtrunA17_Chr8g0377331 24.784 347 201 12 77 386 49 372 1.79e-17 84.0
Msa0218100 MtrunA17_Chr4g0043801 27.491 291 169 10 118 390 159 425 3.94e-16 80.5
Msa0218100 MtrunA17_Chr3g0122631 23.529 442 265 16 26 425 4 414 6.68e-16 79.3
Msa0218100 MtrunA17_Chr1g0200871 27.305 282 149 9 119 366 139 398 2.26e-13 71.6
Msa0218100 MtrunA17_Chr5g0421571 31.915 141 83 4 69 209 139 266 2.28e-13 70.9
Msa0218100 MtrunA17_Chr3g0092341 24.621 264 166 8 118 371 186 426 4.76e-13 70.9
Msa0218100 MtrunA17_Chr7g0254391 23.659 410 240 16 52 418 95 474 8.27e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218100 AT2G28110.1 62.528 443 139 4 9 425 3 444 0.0 586
Msa0218100 AT5G22940.1 64.508 417 134 4 27 430 49 464 0.0 574
Msa0218100 AT5G22940.2 60.314 446 134 4 27 430 49 493 0.0 561
Msa0218100 AT5G61840.1 44.505 364 190 6 58 418 33 387 3.07e-104 315
Msa0218100 AT1G27440.1 42.818 362 199 5 58 418 30 384 7.60e-102 309
Msa0218100 AT4G38040.1 28.090 356 211 14 69 414 96 416 2.92e-31 124
Msa0218100 AT4G38040.2 28.399 331 188 12 69 386 96 390 4.77e-30 120
Msa0218100 AT3G57630.3 26.852 432 222 15 60 413 331 746 1.55e-29 122
Msa0218100 AT3G57630.1 26.852 432 222 15 60 413 331 746 1.55e-29 122
Msa0218100 AT3G57630.2 26.852 432 222 15 60 413 329 744 1.59e-29 122
Msa0218100 AT5G33290.1 26.944 360 217 12 72 414 159 489 3.25e-29 119
Msa0218100 AT4G16745.1 28.814 295 177 7 120 398 240 517 3.25e-28 117
Msa0218100 AT4G16745.2 28.956 297 178 7 120 400 240 519 5.72e-28 116
Msa0218100 AT5G20260.3 26.722 363 220 13 66 414 21 351 1.50e-27 112
Msa0218100 AT5G20260.2 26.431 367 224 13 62 414 122 456 1.05e-26 112
Msa0218100 AT5G20260.1 26.966 356 214 13 73 414 1 324 1.13e-26 110
Msa0218100 AT3G42180.1 25.135 370 231 10 62 414 119 459 2.64e-26 110
Msa0218100 AT5G11130.2 26.230 305 196 8 116 414 42 323 3.95e-26 108
Msa0218100 AT5G11130.1 25.459 381 227 13 59 414 119 467 4.98e-26 110
Msa0218100 AT3G42180.4 25.348 359 222 10 73 414 1 330 1.23e-25 107
Msa0218100 AT5G03795.1 26.146 371 217 10 62 414 175 506 2.61e-25 108
Msa0218100 AT5G25310.1 25.485 361 235 12 62 414 136 470 1.88e-24 105
Msa0218100 AT5G16890.1 26.190 378 207 16 77 411 118 466 4.18e-24 105
Msa0218100 AT5G11610.1 29.429 333 178 13 73 386 216 510 2.56e-23 102
Msa0218100 AT5G37000.1 26.415 318 202 9 73 383 222 514 9.95e-23 101
Msa0218100 AT3G07620.1 25.641 351 227 12 71 414 126 449 1.58e-21 97.1
Msa0218100 AT5G19670.1 26.462 359 211 13 73 414 277 599 3.91e-21 96.3
Msa0218100 AT5G19670.2 26.462 359 211 13 73 414 277 599 3.91e-21 96.3
Msa0218100 AT4G32790.1 27.247 356 212 14 73 414 263 585 6.24e-21 95.9
Msa0218100 AT4G32790.3 27.247 356 212 14 73 414 263 585 6.24e-21 95.9
Msa0218100 AT4G32790.2 27.247 356 212 14 73 414 263 585 6.24e-21 95.9
Msa0218100 AT5G25820.1 27.606 355 214 14 73 414 320 644 2.67e-20 94.0
Msa0218100 AT1G67410.1 24.750 400 226 14 28 387 9 373 7.63e-18 85.5
Msa0218100 AT1G74680.1 26.042 384 220 15 77 418 75 436 4.02e-16 80.5
Msa0218100 AT5G44930.3 22.304 408 259 10 4 384 1 377 3.99e-15 77.0
Msa0218100 AT3G45400.1 26.797 306 188 11 119 411 154 436 5.54e-15 77.0
Msa0218100 AT5G44930.2 22.304 408 259 10 4 384 1 377 5.86e-15 77.0
Msa0218100 AT5G44930.1 22.304 408 259 10 4 384 1 377 5.86e-15 77.0
Msa0218100 AT3G03650.1 24.458 323 207 10 102 411 176 474 4.75e-14 74.3

Find 144 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTTGGGTATGGGAATT+TGG 0.110453 2_1:-65155209 Msa0218100:three_prime_UTR
AACAATGATTGGCTTGTAAA+TGG 0.183723 2_1:-65157546 Msa0218100:CDS
CATATTACAAATGGCTTCTT+TGG 0.197461 2_1:-65157743 Msa0218100:CDS
TACACGTGGTGGAATGTTAT+TGG 0.205370 2_1:-65155543 Msa0218100:three_prime_UTR
TGAACGGACGGCGAGATATT+TGG 0.213752 2_1:-65156833 Msa0218100:CDS
TCGCCACCGAATATCCATTT+TGG 0.223737 2_1:-65157326 Msa0218100:CDS
GCCACGTGGCATCCCCTTCT+TGG 0.225858 2_1:+65155640 None:intergenic
GGCTTTCTTTAGAGGGAAAA+TGG 0.259284 2_1:-65156811 Msa0218100:CDS
CAATTGTGTTGCAGACATTT+GGG 0.271293 2_1:-65156953 Msa0218100:CDS
TTCGTCGCTTCTCATGATTT+TGG 0.301970 2_1:-65157276 Msa0218100:CDS
AGCTGATTTCTTCTTTGTTC+CGG 0.303595 2_1:-65157436 Msa0218100:CDS
GATATTTGGGCTTTCTTTAG+AGG 0.303718 2_1:-65156819 Msa0218100:CDS
TTTAATTTGGTACTTGAAGT+TGG 0.308446 2_1:-65155223 Msa0218100:three_prime_UTR
TCAATTGTGTTGCAGACATT+TGG 0.318671 2_1:-65156954 Msa0218100:CDS
ATATCTACTCTATGTCTCAT+AGG 0.321852 2_1:+65155382 None:intergenic
ATTACAGCTAGGAATTTCTT+TGG 0.339615 2_1:-65155443 Msa0218100:three_prime_UTR
GAACGGACGGCGAGATATTT+GGG 0.348869 2_1:-65156832 Msa0218100:CDS
CCTCTGCTTCGATTCCAAAA+TGG 0.352642 2_1:+65157312 None:intergenic
GATTCTTCTGAATTTGAGTT+AGG 0.355459 2_1:+65155709 None:intergenic
TTTGTTTGTAAAGGAGGATA+AGG 0.361147 2_1:-65157012 Msa0218100:intron
ATTGAATTCTTCAATATTTG+TGG 0.363156 2_1:+65156972 None:intergenic
ACACGTGGTGGAATGTTATT+GGG 0.365435 2_1:-65155542 Msa0218100:three_prime_UTR
TGCAACTTCAGCACTGTTAA+CGG 0.369770 2_1:-65157402 Msa0218100:CDS
CTTCAAAATACAACAATGAT+TGG 0.372602 2_1:-65157557 Msa0218100:CDS
GAAGTTGGGTATGGGAATTT+GGG 0.386150 2_1:-65155208 Msa0218100:three_prime_UTR
ACCACGTGTAATTTCACAAT+TGG 0.386592 2_1:+65155556 None:intergenic
TGGTATGTCTTGTGGGTCAT+TGG 0.394408 2_1:+65157680 None:intergenic
CCGATCGTGCAATTTCCATT+TGG 0.397921 2_1:+65155937 None:intergenic
TCGATTCCAAAATGGATATT+CGG 0.399390 2_1:+65157320 None:intergenic
GGCGAGTGCGAACCGAGATA+TGG 0.400049 2_1:-65156016 Msa0218100:CDS
TTAATTTGGTACTTGAAGTT+GGG 0.401304 2_1:-65155222 Msa0218100:three_prime_UTR
GCCACCGTGAGTGATATTTC+CGG 0.405716 2_1:+65155786 None:intergenic
CATTTCACAGTAGAGGAAAA+TGG 0.406466 2_1:+65155810 None:intergenic
TTGGTACTTGAAGTTGGGTA+TGG 0.409813 2_1:-65155217 Msa0218100:three_prime_UTR
AAGAAGAAATCAGCTTCATA+AGG 0.417900 2_1:+65157444 None:intergenic
TTGAAATGATTGATGAATAT+AGG 0.429858 2_1:-65155183 Msa0218100:three_prime_UTR
GCACGGGCATGTCCAATTGC+CGG 0.435454 2_1:+65157375 None:intergenic
GGGTCCATTGATGAGGTGGC+AGG 0.440189 2_1:-65155336 Msa0218100:three_prime_UTR
TATTCCATCGGCAATTATAA+CGG 0.440851 2_1:+65155839 None:intergenic
GGCATTTATTGCTCAGAAGA+AGG 0.442653 2_1:-65155422 Msa0218100:three_prime_UTR
ACTTTCTCCGGCGGGACATA+AGG 0.442731 2_1:+65156882 None:intergenic
CTACGTGGATGCGTTGGCAT+TGG 0.458114 2_1:-65155486 Msa0218100:three_prime_UTR
ACAATGATTGGCTTGTAAAT+GGG 0.476562 2_1:-65157545 Msa0218100:CDS
GTTGAATTTCCTCCATATCT+CGG 0.477416 2_1:+65156004 None:intergenic
TTACATTCTGATCCATCATA+AGG 0.485167 2_1:-65157911 Msa0218100:five_prime_UTR
GGACCTCACTGTTGAAAAGT+AGG 0.486436 2_1:+65155661 None:intergenic
TGGTACTTGAAGTTGGGTAT+GGG 0.487158 2_1:-65155216 Msa0218100:three_prime_UTR
AAAACCTGCCACCTCATCAA+TGG 0.491703 2_1:+65155332 None:intergenic
ATTCCATCGGCAATTATAAC+GGG 0.492324 2_1:+65155840 None:intergenic
GTGTATCGGTTACCATATGC+TGG 0.493143 2_1:+65157942 None:intergenic
GTCACGTGGTTGCCTTATGA+TGG 0.496848 2_1:+65157899 None:intergenic
AGAGGAGCAAAACCAGCATA+TGG 0.499752 2_1:-65157954 None:intergenic
GGCTAGAAACCATGGACCTA+TGG 0.501853 2_1:+65155577 None:intergenic
TCTGTCTATGTCCACTAGGG+TGG 0.502220 2_1:-65155911 Msa0218100:CDS
TGAATATAGGAGAGAGATGT+GGG 0.502417 2_1:-65155170 Msa0218100:three_prime_UTR
GAGGATAAGGCAATGGGTGA+TGG 0.502896 2_1:-65156999 Msa0218100:CDS
TTCAACAGTGAGGTCCAAGA+AGG 0.503528 2_1:-65155654 Msa0218100:CDS
TTTGACTACTCACCAGGGTA+TGG 0.511672 2_1:+65157244 None:intergenic
AGAAGAAATCAGCTTCATAA+GGG 0.512054 2_1:+65157445 None:intergenic
GTATTCTGTCTATGTCCACT+AGG 0.512595 2_1:-65155915 Msa0218100:CDS
TTCCGGCCATTTCACAGTAG+AGG 0.516050 2_1:+65155803 None:intergenic
TTCCTCTACTGTGAAATGGC+CGG 0.518492 2_1:-65155805 Msa0218100:CDS
ATGTAACCCGGCGGGTGTAT+CGG 0.519061 2_1:+65157928 None:intergenic
CTCAAATTCAGAAGAATCTG+TGG 0.521949 2_1:-65155704 Msa0218100:CDS
GTCATTGGTTATGAGATAAG+AGG 0.523787 2_1:+65157695 None:intergenic
TCCTCGAAACTTTCTCCGGC+GGG 0.523894 2_1:+65156874 None:intergenic
ATATTTGGGCTTTCTTTAGA+GGG 0.526071 2_1:-65156818 Msa0218100:CDS
GGGTGGGTCCATTGATGAGG+TGG 0.526216 2_1:-65155340 Msa0218100:three_prime_UTR
CCAAATGGAAATTGCACGAT+CGG 0.526424 2_1:-65155937 Msa0218100:CDS
ATATCTCGCCGTCCGTTCAC+CGG 0.527175 2_1:+65156837 None:intergenic
GAAGAAGGAATTCTGATGGT+GGG 0.529622 2_1:-65155407 Msa0218100:three_prime_UTR
TATCTACTCTATGTCTCATA+GGG 0.532667 2_1:+65155383 None:intergenic
CATGAGAAGCGACGAAAACA+TGG 0.539964 2_1:+65157283 None:intergenic
CGCGGAGGAAATGAGAGCAC+GGG 0.543844 2_1:+65157359 None:intergenic
TCTACAAGCCTCGGACTCCA+TGG 0.543867 2_1:+65155885 None:intergenic
GGAATTAGAATTTGAGACAA+TGG 0.547428 2_1:+65157644 None:intergenic
GTAAAGGAGGATAAGGCAAT+GGG 0.549011 2_1:-65157005 Msa0218100:intron
TAGAATCCTTTGTTCTTGAG+TGG 0.549820 2_1:+65157840 None:intergenic
TGGCAATGACAACTATAAAG+TGG 0.550975 2_1:-65155466 Msa0218100:three_prime_UTR
AAGTTGCAAGAAACATAAAC+CGG 0.551101 2_1:+65157417 None:intergenic
TGGTGCATGTTTCCATACCC+TGG 0.551233 2_1:-65157256 Msa0218100:intron
AGTTTCCTACGTGGATGCGT+TGG 0.554385 2_1:-65155492 Msa0218100:three_prime_UTR
TGTAAAGGAGGATAAGGCAA+TGG 0.558528 2_1:-65157006 Msa0218100:intron
CTGTCTATGTCCACTAGGGT+GGG 0.559699 2_1:-65155910 Msa0218100:CDS
TTTAGTCTAAGTCAGAAGCT+TGG 0.565244 2_1:-65155609 Msa0218100:CDS
TCAACAGTGAGGTCCAAGAA+GGG 0.566785 2_1:-65155653 Msa0218100:CDS
GACCTCACTGTTGAAAAGTA+GGG 0.567183 2_1:+65155662 None:intergenic
GGTGGGTGGGTCCATTGATG+AGG 0.571396 2_1:-65155343 Msa0218100:three_prime_UTR
GGTGGGCACCATGGAGTCCG+AGG 0.573935 2_1:-65155893 Msa0218100:CDS
CTTGTAGAATCAGTTGCACT+CGG 0.574677 2_1:-65155870 Msa0218100:CDS
TCACAATTGGCTAGAAACCA+TGG 0.575572 2_1:+65155569 None:intergenic
TAGGTCCTACCATAGGTCCA+TGG 0.575747 2_1:-65155586 Msa0218100:CDS
ATGAATATAGGAGAGAGATG+TGG 0.577671 2_1:-65155171 Msa0218100:three_prime_UTR
CTTGAGAAAGCTCCGGTGAA+CGG 0.579154 2_1:-65156849 Msa0218100:CDS
GCAACTGATTCTACAAGCCT+CGG 0.580697 2_1:+65155876 None:intergenic
CCGCGGAGGAAATGAGAGCA+CGG 0.583735 2_1:+65157358 None:intergenic
AGAAAGCTCCGGTGAACGGA+CGG 0.583778 2_1:-65156845 Msa0218100:CDS
GTCCACTAGGGTGGGCACCA+TGG 0.587305 2_1:-65155902 Msa0218100:CDS
CTATAAAGTGGATTACAGCT+AGG 0.589040 2_1:-65155454 Msa0218100:three_prime_UTR
AGCGTCCTCGAAACTTTCTC+CGG 0.597937 2_1:+65156870 None:intergenic
CAACAGTGAGGTCCAAGAAG+GGG 0.599472 2_1:-65155652 Msa0218100:CDS
GGATCAGAATGTAACCCGGC+GGG 0.601768 2_1:+65157920 None:intergenic
GAGGACGCTTGAGAAAGCTC+CGG 0.603668 2_1:-65156856 Msa0218100:CDS
GTCTAAGTCAGAAGCTTGGT+AGG 0.604636 2_1:-65155605 Msa0218100:CDS
GAGTGCGAACCGAGATATGG+AGG 0.605750 2_1:-65156013 Msa0218100:CDS
TATTCTGTCTATGTCCACTA+GGG 0.612391 2_1:-65155914 Msa0218100:CDS
AAATGGCAAACGTATTCCAT+CGG 0.615484 2_1:+65155827 None:intergenic
TCCCGCCGGAGAAAGTTTCG+AGG 0.616182 2_1:-65156875 Msa0218100:CDS
CTCAGAAGAAGGAATTCTGA+TGG 0.617316 2_1:-65155411 Msa0218100:three_prime_UTR
AGAAGAAGGAATTCTGATGG+TGG 0.617831 2_1:-65155408 Msa0218100:three_prime_UTR
CTCCATGGTGCCCACCCTAG+TGG 0.618009 2_1:+65155900 None:intergenic
TTCTCCGGCGGGACATAAGG+CGG 0.619669 2_1:+65156885 None:intergenic
GGCGATGAGTTCAACCGCGG+AGG 0.624680 2_1:+65157344 None:intergenic
GTCCTCGAAACTTTCTCCGG+CGG 0.628866 2_1:+65156873 None:intergenic
AATTGTGAAATTACACGTGG+TGG 0.632003 2_1:-65155554 Msa0218100:exon
TTGTCAAGATGTTGAGAATG+TGG 0.637717 2_1:-65156913 Msa0218100:CDS
AAGAAGGAATTCTGATGGTG+GGG 0.639354 2_1:-65155406 Msa0218100:three_prime_UTR
ATGGTAACCGATACACCCGC+CGG 0.639793 2_1:-65157935 Msa0218100:five_prime_UTR
AGAAACCATGGACCTATGGT+AGG 0.643221 2_1:+65155581 None:intergenic
TGGTAACCGATACACCCGCC+GGG 0.643380 2_1:-65157934 Msa0218100:five_prime_UTR
ATTCCAAAATGGATATTCGG+TGG 0.644063 2_1:+65157323 None:intergenic
AATTGTGTTGCAGACATTTG+GGG 0.645114 2_1:-65156952 Msa0218100:CDS
ACAAAACCACTCAAGAACAA+AGG 0.645432 2_1:-65157846 Msa0218100:five_prime_UTR
TTTGAGTTTCAGAAGTCACG+TGG 0.647237 2_1:+65157885 None:intergenic
CCGTGCTCTCATTTCCTCCG+CGG 0.649042 2_1:-65157358 Msa0218100:CDS
TCAAATTCAGAAGAATCTGT+GGG 0.650477 2_1:-65155703 Msa0218100:CDS
TCCAAGAAGGGGATGCCACG+TGG 0.655039 2_1:-65155641 Msa0218100:CDS
CGACAAAATTCTCAAGCGCG+TGG 0.655151 2_1:-65155742 Msa0218100:CDS
GTGCCCGTTATAATTGCCGA+TGG 0.661063 2_1:-65155843 Msa0218100:CDS
ATGGCCGGAAATATCACTCA+CGG 0.666857 2_1:-65155790 Msa0218100:CDS
TGAGTTATGAGAAACAACGT+TGG 0.674637 2_1:+65157623 None:intergenic
TGATGGATCAGAATGTAACC+CGG 0.675513 2_1:+65157916 None:intergenic
AGCTTGGTAGGTCCTACCAT+AGG 0.686856 2_1:-65155593 Msa0218100:CDS
ACATTCTCAACATCTTGACA+AGG 0.692808 2_1:+65156915 None:intergenic
ATGATTGGCTTGTAAATGGG+AGG 0.708014 2_1:-65157542 Msa0218100:CDS
CAATGCCAACGCATCCACGT+AGG 0.713905 2_1:+65155487 None:intergenic
GCCAATTGTGAAATTACACG+TGG 0.723864 2_1:-65155557 Msa0218100:exon
ATGAAGCTTAGTTTCCTACG+TGG 0.725233 2_1:-65155501 Msa0218100:three_prime_UTR
ACGGTGGCAGAGAAAGACGT+AGG 0.728097 2_1:-65155771 Msa0218100:CDS
TGGATCAGAATGTAACCCGG+CGG 0.732273 2_1:+65157919 None:intergenic
TATACCGCCTTATGTCCCGC+CGG 0.733413 2_1:-65156889 Msa0218100:CDS
GGTGGCGATGAGTTCAACCG+CGG 0.757490 2_1:+65157341 None:intergenic
ACTAAATAATACTTGCCACG+TGG 0.759736 2_1:+65155626 None:intergenic
GCCGGAAATATCACTCACGG+TGG 0.764283 2_1:-65155787 Msa0218100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATATATTTTTGTATTTTT+AGG - chr2_1:65157709-65157728 Msa0218100:CDS 10.0%
!!! GATATATTTTTGTATTTTTA+GGG - chr2_1:65157710-65157729 Msa0218100:CDS 10.0%
!!! TCAATAAAGAAATTTTAATT+TGG - chr2_1:65157838-65157857 Msa0218100:five_prime_UTR 10.0%
!!! TCTTTTTTACATATTACAAA+TGG - chr2_1:65155322-65155341 Msa0218100:three_prime_UTR 15.0%
!!! TTTGAATTTTTTGTTTGTAA+AGG - chr2_1:65156053-65156072 Msa0218100:intron 15.0%
!!! TTTTTACACGTTTTAAATTA+AGG - chr2_1:65156379-65156398 Msa0218100:intron 15.0%
!! TTTAACAGAAATTTAAATCA+TGG + chr2_1:65156433-65156452 None:intergenic 15.0%
!!! ATCTTTTGAATGTAATTATT+TGG - chr2_1:65156898-65156917 Msa0218100:CDS 15.0%
!!! AAAAGAAACATGTTTTATGA+TGG + chr2_1:65155308-65155327 None:intergenic 20.0%
!! AGATATGTTGAAAAATTTGA+AGG - chr2_1:65155476-65155495 Msa0218100:three_prime_UTR 20.0%
!! ATTGAATTCTTCAATATTTG+TGG + chr2_1:65156105-65156124 None:intergenic 20.0%
!!! TAGTTAATGATTTTATTGCA+GGG + chr2_1:65156346-65156365 None:intergenic 20.0%
!!! TTAGTTAATGATTTTATTGC+AGG + chr2_1:65156347-65156366 None:intergenic 20.0%
!!! AGGTGATTTTAATCAAATTA+TGG - chr2_1:65156399-65156418 Msa0218100:intron 20.0%
!!! TTTAAGTAAAAGCTACATTT+TGG + chr2_1:65156559-65156578 None:intergenic 20.0%
!! ATTTCTTAAGATGTGTTTAT+AGG + chr2_1:65156648-65156667 None:intergenic 20.0%
!!! TTTTCGTACAAATATACATT+TGG - chr2_1:65156778-65156797 Msa0218100:CDS 20.0%
!! AATCTATTAAACTCATTGAA+TGG - chr2_1:65156813-65156832 Msa0218100:CDS 20.0%
!! CAATTACATTAATTCATCAA+CGG + chr2_1:65157017-65157036 None:intergenic 20.0%
!!! ATATTTTTGTATTTTTAGGG+TGG - chr2_1:65157713-65157732 Msa0218100:CDS 20.0%
!!! TATTTTTGTATTTTTAGGGT+GGG - chr2_1:65157714-65157733 Msa0218100:CDS 20.0%
!! TTGAAATGATTGATGAATAT+AGG - chr2_1:65157891-65157910 Msa0218100:five_prime_UTR 20.0%
!!! ACAATGGTTTTTGTTTTTGT+TGG + chr2_1:65155417-65155436 None:intergenic 25.0%
!!! CAATCATTGTTGTATTTTGA+AGG + chr2_1:65155519-65155538 None:intergenic 25.0%
! CTTCAAAATACAACAATGAT+TGG - chr2_1:65155517-65155536 Msa0218100:three_prime_UTR 25.0%
!!! GAATTTTTTGTTTGTAAAGG+AGG - chr2_1:65156056-65156075 Msa0218100:intron 25.0%
!!! ACTGTGTTTTTTATTTGTGA+AGG - chr2_1:65157037-65157056 Msa0218100:intron 25.0%
!!! TTTAATTTGGTACTTGAAGT+TGG - chr2_1:65157851-65157870 Msa0218100:five_prime_UTR 25.0%
!! TTAATTTGGTACTTGAAGTT+GGG - chr2_1:65157852-65157871 Msa0218100:five_prime_UTR 25.0%
TTACATTCTGATCCATCATA+AGG - chr2_1:65155163-65155182 Msa0218100:three_prime_UTR 30.0%
CATATTACAAATGGCTTCTT+TGG - chr2_1:65155331-65155350 Msa0218100:three_prime_UTR 30.0%
! TTTTTGTTGGTATGTCTTGT+GGG + chr2_1:65155404-65155423 None:intergenic 30.0%
! GGAATTAGAATTTGAGACAA+TGG + chr2_1:65155433-65155452 None:intergenic 30.0%
!! AACAATGATTGGCTTGTAAA+TGG - chr2_1:65155528-65155547 Msa0218100:three_prime_UTR 30.0%
!! ACAATGATTGGCTTGTAAAT+GGG - chr2_1:65155529-65155548 Msa0218100:three_prime_UTR 30.0%
AGAAGAAATCAGCTTCATAA+GGG + chr2_1:65155632-65155651 None:intergenic 30.0%
AAGAAGAAATCAGCTTCATA+AGG + chr2_1:65155633-65155652 None:intergenic 30.0%
AAGTTGCAAGAAACATAAAC+CGG + chr2_1:65155660-65155679 None:intergenic 30.0%
TCGATTCCAAAATGGATATT+CGG + chr2_1:65155757-65155776 None:intergenic 30.0%
GAAGTCAAACTTTGATATCA+CGG + chr2_1:65155979-65155998 None:intergenic 30.0%
TTTGTTTGTAAAGGAGGATA+AGG - chr2_1:65156062-65156081 Msa0218100:intron 30.0%
ATATTTGGGCTTTCTTTAGA+GGG - chr2_1:65156256-65156275 Msa0218100:intron 30.0%
GTTCATCCTAAAAATGTTAG+TGG - chr2_1:65156288-65156307 Msa0218100:intron 30.0%
ATCCTAAAAATGTTAGTGGA+CGG - chr2_1:65156292-65156311 Msa0218100:intron 30.0%
CTACCTTTGTGAATTGATAT+GGG - chr2_1:65156957-65156976 Msa0218100:CDS 30.0%
TAACCCATATCAATTCACAA+AGG + chr2_1:65156963-65156982 None:intergenic 30.0%
! ATTCATTCTGCATTGCATTT+TGG - chr2_1:65156988-65157007 Msa0218100:CDS 30.0%
!!! TAGATTTTCAGGTTACCAAA+TGG - chr2_1:65157122-65157141 Msa0218100:intron 30.0%
TATTCCATCGGCAATTATAA+CGG + chr2_1:65157238-65157257 None:intergenic 30.0%
! GATTCTTCTGAATTTGAGTT+AGG + chr2_1:65157368-65157387 None:intergenic 30.0%
TCAAATTCAGAAGAATCTGT+GGG - chr2_1:65157371-65157390 Msa0218100:CDS 30.0%
ATTACAGCTAGGAATTTCTT+TGG - chr2_1:65157631-65157650 Msa0218100:CDS 30.0%
TATCTACTCTATGTCTCATA+GGG + chr2_1:65157694-65157713 None:intergenic 30.0%
ATATCTACTCTATGTCTCAT+AGG + chr2_1:65157695-65157714 None:intergenic 30.0%
ACAAAACCACTCAAGAACAA+AGG - chr2_1:65155228-65155247 Msa0218100:three_prime_UTR 35.0%
TAGAATCCTTTGTTCTTGAG+TGG + chr2_1:65155237-65155256 None:intergenic 35.0%
!!! GTTTTATGATGGTCTTGTTG+AGG + chr2_1:65155297-65155316 None:intergenic 35.0%
! GTCATTGGTTATGAGATAAG+AGG + chr2_1:65155382-65155401 None:intergenic 35.0%
! GTTTTTGTTGGTATGTCTTG+TGG + chr2_1:65155405-65155424 None:intergenic 35.0%
TGAGTTATGAGAAACAACGT+TGG + chr2_1:65155454-65155473 None:intergenic 35.0%
!! CACATCACTTTTCAACAATG+CGG + chr2_1:65155598-65155617 None:intergenic 35.0%
! AGCTGATTTCTTCTTTGTTC+CGG - chr2_1:65155638-65155657 Msa0218100:CDS 35.0%
ATTCCAAAATGGATATTCGG+TGG + chr2_1:65155754-65155773 None:intergenic 35.0%
TCAATTGTGTTGCAGACATT+TGG - chr2_1:65156120-65156139 Msa0218100:intron 35.0%
CAATTGTGTTGCAGACATTT+GGG - chr2_1:65156121-65156140 Msa0218100:intron 35.0%
AATTGTGTTGCAGACATTTG+GGG - chr2_1:65156122-65156141 Msa0218100:intron 35.0%
ACATTCTCAACATCTTGACA+AGG + chr2_1:65156162-65156181 None:intergenic 35.0%
TTGTCAAGATGTTGAGAATG+TGG - chr2_1:65156161-65156180 Msa0218100:intron 35.0%
GATATTTGGGCTTTCTTTAG+AGG - chr2_1:65156255-65156274 Msa0218100:intron 35.0%
! TACCGTCCACTAACATTTTT+AGG + chr2_1:65156297-65156316 None:intergenic 35.0%
! TTGTGTTATTAACACGTGCA+TGG - chr2_1:65156743-65156762 Msa0218100:intron 35.0%
ATGCTATCTCAATCAAAGTG+AGG + chr2_1:65156874-65156893 None:intergenic 35.0%
GCTACCTTTGTGAATTGATA+TGG - chr2_1:65156956-65156975 Msa0218100:CDS 35.0%
GTTGAATTTCCTCCATATCT+CGG + chr2_1:65157073-65157092 None:intergenic 35.0%
! CTACAACGACATAGATTTTC+AGG - chr2_1:65157111-65157130 Msa0218100:intron 35.0%
TATTCTGTCTATGTCCACTA+GGG - chr2_1:65157160-65157179 Msa0218100:intron 35.0%
ATTCCATCGGCAATTATAAC+GGG + chr2_1:65157237-65157256 None:intergenic 35.0%
AAATGGCAAACGTATTCCAT+CGG + chr2_1:65157250-65157269 None:intergenic 35.0%
CATTTCACAGTAGAGGAAAA+TGG + chr2_1:65157267-65157286 None:intergenic 35.0%
! CATTTTCCTCTACTGTGAAA+TGG - chr2_1:65157265-65157284 Msa0218100:CDS 35.0%
CTCAAATTCAGAAGAATCTG+TGG - chr2_1:65157370-65157389 Msa0218100:CDS 35.0%
ACTAAATAATACTTGCCACG+TGG + chr2_1:65157451-65157470 None:intergenic 35.0%
TTTAGTCTAAGTCAGAAGCT+TGG - chr2_1:65157465-65157484 Msa0218100:CDS 35.0%
ACCACGTGTAATTTCACAAT+TGG + chr2_1:65157521-65157540 None:intergenic 35.0%
AATTGTGAAATTACACGTGG+TGG - chr2_1:65157520-65157539 Msa0218100:CDS 35.0%
TGGCAATGACAACTATAAAG+TGG - chr2_1:65157608-65157627 Msa0218100:CDS 35.0%
!! CTATAAAGTGGATTACAGCT+AGG - chr2_1:65157620-65157639 Msa0218100:CDS 35.0%
!!! TTTTGTATTTTTAGGGTGGG+TGG - chr2_1:65157717-65157736 Msa0218100:CDS 35.0%
!!! TTTGTATTTTTAGGGTGGGT+GGG - chr2_1:65157718-65157737 Msa0218100:CDS 35.0%
ATGAATATAGGAGAGAGATG+TGG - chr2_1:65157903-65157922 Msa0218100:five_prime_UTR 35.0%
TGAATATAGGAGAGAGATGT+GGG - chr2_1:65157904-65157923 Msa0218100:five_prime_UTR 35.0%
! TGATGGATCAGAATGTAACC+CGG + chr2_1:65155161-65155180 None:intergenic 40.0%
! TTTGAGTTTCAGAAGTCACG+TGG + chr2_1:65155192-65155211 None:intergenic 40.0%
!! ATGATTGGCTTGTAAATGGG+AGG - chr2_1:65155532-65155551 Msa0218100:three_prime_UTR 40.0%
TGCAACTTCAGCACTGTTAA+CGG - chr2_1:65155672-65155691 Msa0218100:CDS 40.0%
! TTCGTCGCTTCTCATGATTT+TGG - chr2_1:65155798-65155817 Msa0218100:CDS 40.0%
TGTAAAGGAGGATAAGGCAA+TGG - chr2_1:65156068-65156087 Msa0218100:intron 40.0%
GTAAAGGAGGATAAGGCAAT+GGG - chr2_1:65156069-65156088 Msa0218100:intron 40.0%
! GGCTTTCTTTAGAGGGAAAA+TGG - chr2_1:65156263-65156282 Msa0218100:intron 40.0%
CCAAATGGAAATTGCACGAT+CGG - chr2_1:65157137-65157156 Msa0218100:intron 40.0%
GTATTCTGTCTATGTCCACT+AGG - chr2_1:65157159-65157178 Msa0218100:intron 40.0%
CTTGTAGAATCAGTTGCACT+CGG - chr2_1:65157204-65157223 Msa0218100:intron 40.0%
GACCTCACTGTTGAAAAGTA+GGG + chr2_1:65157415-65157434 None:intergenic 40.0%
TCACAATTGGCTAGAAACCA+TGG + chr2_1:65157508-65157527 None:intergenic 40.0%
GCCAATTGTGAAATTACACG+TGG - chr2_1:65157517-65157536 Msa0218100:CDS 40.0%
! TACACGTGGTGGAATGTTAT+TGG - chr2_1:65157531-65157550 Msa0218100:CDS 40.0%
! ACACGTGGTGGAATGTTATT+GGG - chr2_1:65157532-65157551 Msa0218100:CDS 40.0%
ATGAAGCTTAGTTTCCTACG+TGG - chr2_1:65157573-65157592 Msa0218100:CDS 40.0%
GGCATTTATTGCTCAGAAGA+AGG - chr2_1:65157652-65157671 Msa0218100:CDS 40.0%
CTCAGAAGAAGGAATTCTGA+TGG - chr2_1:65157663-65157682 Msa0218100:CDS 40.0%
AGAAGAAGGAATTCTGATGG+TGG - chr2_1:65157666-65157685 Msa0218100:CDS 40.0%
GAAGAAGGAATTCTGATGGT+GGG - chr2_1:65157667-65157686 Msa0218100:CDS 40.0%
! AAGAAGGAATTCTGATGGTG+GGG - chr2_1:65157668-65157687 Msa0218100:CDS 40.0%
TTGGTACTTGAAGTTGGGTA+TGG - chr2_1:65157857-65157876 Msa0218100:five_prime_UTR 40.0%
TGGTACTTGAAGTTGGGTAT+GGG - chr2_1:65157858-65157877 Msa0218100:five_prime_UTR 40.0%
TGAAGTTGGGTATGGGAATT+TGG - chr2_1:65157865-65157884 Msa0218100:five_prime_UTR 40.0%
GAAGTTGGGTATGGGAATTT+GGG - chr2_1:65157866-65157885 Msa0218100:five_prime_UTR 40.0%
!! GTGTATCGGTTACCATATGC+TGG + chr2_1:65155135-65155154 None:intergenic 45.0%
TGGTATGTCTTGTGGGTCAT+TGG + chr2_1:65155397-65155416 None:intergenic 45.0%
! CAGCACTGTTAACGGTTTTC+CGG - chr2_1:65155680-65155699 Msa0218100:CDS 45.0%
! GTTAACGGTTTTCCGGCAAT+TGG - chr2_1:65155687-65155706 Msa0218100:CDS 45.0%
! TCGCCACCGAATATCCATTT+TGG - chr2_1:65155748-65155767 Msa0218100:CDS 45.0%
CCTCTGCTTCGATTCCAAAA+TGG + chr2_1:65155765-65155784 None:intergenic 45.0%
!!! CCATTTTGGAATCGAAGCAG+AGG - chr2_1:65155762-65155781 Msa0218100:CDS 45.0%
CATGAGAAGCGACGAAAACA+TGG + chr2_1:65155794-65155813 None:intergenic 45.0%
TTTGACTACTCACCAGGGTA+TGG + chr2_1:65155833-65155852 None:intergenic 45.0%
CGATGTTTGACTACTCACCA+GGG + chr2_1:65155838-65155857 None:intergenic 45.0%
ACGATGTTTGACTACTCACC+AGG + chr2_1:65155839-65155858 None:intergenic 45.0%
CCGATCGTGCAATTTCCATT+TGG + chr2_1:65157140-65157159 None:intergenic 45.0%
! GCAACTGATTCTACAAGCCT+CGG + chr2_1:65157201-65157220 None:intergenic 45.0%
TTCCTCTACTGTGAAATGGC+CGG - chr2_1:65157269-65157288 Msa0218100:CDS 45.0%
ATGGCCGGAAATATCACTCA+CGG - chr2_1:65157284-65157303 Msa0218100:CDS 45.0%
!!! GTAGGGCCTGTCTTGTTTTT+GGG + chr2_1:65157398-65157417 None:intergenic 45.0%
!!! AGTAGGGCCTGTCTTGTTTT+TGG + chr2_1:65157399-65157418 None:intergenic 45.0%
GGACCTCACTGTTGAAAAGT+AGG + chr2_1:65157416-65157435 None:intergenic 45.0%
TTCAACAGTGAGGTCCAAGA+AGG - chr2_1:65157420-65157439 Msa0218100:CDS 45.0%
TCAACAGTGAGGTCCAAGAA+GGG - chr2_1:65157421-65157440 Msa0218100:CDS 45.0%
GTCTAAGTCAGAAGCTTGGT+AGG - chr2_1:65157469-65157488 Msa0218100:CDS 45.0%
AGAAACCATGGACCTATGGT+AGG + chr2_1:65157496-65157515 None:intergenic 45.0%
AAAACCTGCCACCTCATCAA+TGG + chr2_1:65157745-65157764 None:intergenic 45.0%
!!! TGAGGTGGCAGGTTTTGATT+TGG - chr2_1:65157749-65157768 Msa0218100:CDS 45.0%
!! AAAAATATATAATAAATTCA+TGG + chr2_1:65156601-65156620 None:intergenic 5.0%
TGGATCAGAATGTAACCCGG+CGG + chr2_1:65155158-65155177 None:intergenic 50.0%
GTCACGTGGTTGCCTTATGA+TGG + chr2_1:65155178-65155197 None:intergenic 50.0%
TGGTGCATGTTTCCATACCC+TGG - chr2_1:65155818-65155837 Msa0218100:CDS 50.0%
GAGGATAAGGCAATGGGTGA+TGG - chr2_1:65156075-65156094 Msa0218100:intron 50.0%
AGCGTCCTCGAAACTTTCTC+CGG + chr2_1:65156207-65156226 None:intergenic 50.0%
CTTGAGAAAGCTCCGGTGAA+CGG - chr2_1:65156225-65156244 Msa0218100:intron 50.0%
TGAACGGACGGCGAGATATT+TGG - chr2_1:65156241-65156260 Msa0218100:intron 50.0%
GAACGGACGGCGAGATATTT+GGG - chr2_1:65156242-65156261 Msa0218100:intron 50.0%
TCTGTCTATGTCCACTAGGG+TGG - chr2_1:65157163-65157182 Msa0218100:intron 50.0%
CTGTCTATGTCCACTAGGGT+GGG - chr2_1:65157164-65157183 Msa0218100:intron 50.0%
GTGCCCGTTATAATTGCCGA+TGG - chr2_1:65157231-65157250 Msa0218100:intron 50.0%
TTCCGGCCATTTCACAGTAG+AGG + chr2_1:65157274-65157293 None:intergenic 50.0%
GCCACCGTGAGTGATATTTC+CGG + chr2_1:65157291-65157310 None:intergenic 50.0%
CGACAAAATTCTCAAGCGCG+TGG - chr2_1:65157332-65157351 Msa0218100:CDS 50.0%
GTGGGACCCAAAAACAAGAC+AGG - chr2_1:65157389-65157408 Msa0218100:CDS 50.0%
!! GGCCCTACTTTTCAACAGTG+AGG - chr2_1:65157410-65157429 Msa0218100:CDS 50.0%
CAACAGTGAGGTCCAAGAAG+GGG - chr2_1:65157422-65157441 Msa0218100:CDS 50.0%
AGCTTGGTAGGTCCTACCAT+AGG - chr2_1:65157481-65157500 Msa0218100:CDS 50.0%
TAGGTCCTACCATAGGTCCA+TGG - chr2_1:65157488-65157507 Msa0218100:CDS 50.0%
GGCTAGAAACCATGGACCTA+TGG + chr2_1:65157500-65157519 None:intergenic 50.0%
AGTTTCCTACGTGGATGCGT+TGG - chr2_1:65157582-65157601 Msa0218100:CDS 50.0%
ATGGTAACCGATACACCCGC+CGG - chr2_1:65155139-65155158 Msa0218100:three_prime_UTR 55.0%
ATGTAACCCGGCGGGTGTAT+CGG + chr2_1:65155149-65155168 None:intergenic 55.0%
GGATCAGAATGTAACCCGGC+GGG + chr2_1:65155157-65155176 None:intergenic 55.0%
TATACCGCCTTATGTCCCGC+CGG - chr2_1:65156185-65156204 Msa0218100:intron 55.0%
ACTTTCTCCGGCGGGACATA+AGG + chr2_1:65156195-65156214 None:intergenic 55.0%
TCCTCGAAACTTTCTCCGGC+GGG + chr2_1:65156203-65156222 None:intergenic 55.0%
GTCCTCGAAACTTTCTCCGG+CGG + chr2_1:65156204-65156223 None:intergenic 55.0%
GAGGACGCTTGAGAAAGCTC+CGG - chr2_1:65156218-65156237 Msa0218100:intron 55.0%
AGAAAGCTCCGGTGAACGGA+CGG - chr2_1:65156229-65156248 Msa0218100:intron 55.0%
ATATCTCGCCGTCCGTTCAC+CGG + chr2_1:65156240-65156259 None:intergenic 55.0%
GAGTGCGAACCGAGATATGG+AGG - chr2_1:65157061-65157080 Msa0218100:intron 55.0%
TCTACAAGCCTCGGACTCCA+TGG + chr2_1:65157192-65157211 None:intergenic 55.0%
GCCGGAAATATCACTCACGG+TGG - chr2_1:65157287-65157306 Msa0218100:CDS 55.0%
ACGGTGGCAGAGAAAGACGT+AGG - chr2_1:65157303-65157322 Msa0218100:CDS 55.0%
CAATGCCAACGCATCCACGT+AGG + chr2_1:65157590-65157609 None:intergenic 55.0%
! CTACGTGGATGCGTTGGCAT+TGG - chr2_1:65157588-65157607 Msa0218100:CDS 55.0%
TGGTAACCGATACACCCGCC+GGG - chr2_1:65155140-65155159 Msa0218100:three_prime_UTR 60.0%
GCACGGGCATGTCCAATTGC+CGG + chr2_1:65155702-65155721 None:intergenic 60.0%
! CGCGGAGGAAATGAGAGCAC+GGG + chr2_1:65155718-65155737 None:intergenic 60.0%
CCGCGGAGGAAATGAGAGCA+CGG + chr2_1:65155719-65155738 None:intergenic 60.0%
CCGTGCTCTCATTTCCTCCG+CGG - chr2_1:65155716-65155735 Msa0218100:CDS 60.0%
GGTGGCGATGAGTTCAACCG+CGG + chr2_1:65155736-65155755 None:intergenic 60.0%
TTCTCCGGCGGGACATAAGG+CGG + chr2_1:65156192-65156211 None:intergenic 60.0%
TCCCGCCGGAGAAAGTTTCG+AGG - chr2_1:65156199-65156218 Msa0218100:intron 60.0%
GGCGAGTGCGAACCGAGATA+TGG - chr2_1:65157058-65157077 Msa0218100:intron 60.0%
TCCAAGAAGGGGATGCCACG+TGG - chr2_1:65157433-65157452 Msa0218100:CDS 60.0%
GGTGGGTGGGTCCATTGATG+AGG - chr2_1:65157731-65157750 Msa0218100:CDS 60.0%
GGGTGGGTCCATTGATGAGG+TGG - chr2_1:65157734-65157753 Msa0218100:CDS 60.0%
GGGTCCATTGATGAGGTGGC+AGG - chr2_1:65157738-65157757 Msa0218100:CDS 60.0%
GGCGATGAGTTCAACCGCGG+AGG + chr2_1:65155733-65155752 None:intergenic 65.0%
GTCCACTAGGGTGGGCACCA+TGG - chr2_1:65157172-65157191 Msa0218100:intron 65.0%
CTCCATGGTGCCCACCCTAG+TGG + chr2_1:65157177-65157196 None:intergenic 65.0%
GCCACGTGGCATCCCCTTCT+TGG + chr2_1:65157437-65157456 None:intergenic 65.0%
GGTGGGCACCATGGAGTCCG+AGG - chr2_1:65157181-65157200 Msa0218100:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 65155126 65157970 65155126 ID=Msa0218100;Name=Msa0218100
chr2_1 mRNA 65155126 65157970 65155126 ID=Msa0218100-mRNA-1;Parent=Msa0218100;Name=Msa0218100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=138|1|1|1|1|1|3|443|431
chr2_1 exon 65155126 65156037 65155126 ID=Msa0218100-mRNA-1:exon:18113;Parent=Msa0218100-mRNA-1
chr2_1 exon 65156771 65157021 65156771 ID=Msa0218100-mRNA-1:exon:18112;Parent=Msa0218100-mRNA-1
chr2_1 exon 65157257 65157970 65157257 ID=Msa0218100-mRNA-1:exon:18111;Parent=Msa0218100-mRNA-1
chr2_1 five_prime_UTR 65157833 65157970 65157833 ID=Msa0218100-mRNA-1:five_prime_utr;Parent=Msa0218100-mRNA-1
chr2_1 CDS 65157257 65157832 65157257 ID=Msa0218100-mRNA-1:cds;Parent=Msa0218100-mRNA-1
chr2_1 CDS 65156771 65157021 65156771 ID=Msa0218100-mRNA-1:cds;Parent=Msa0218100-mRNA-1
chr2_1 CDS 65155569 65156037 65155569 ID=Msa0218100-mRNA-1:cds;Parent=Msa0218100-mRNA-1
chr2_1 three_prime_UTR 65155126 65155568 65155126 ID=Msa0218100-mRNA-1:three_prime_utr;Parent=Msa0218100-mRNA-1
Gene Sequence

>Msa0218100

ATGAAACTCATGAACAAACATAACACAAAACCTCAACAAGACCATCATAAAACATGTTTCTTTTTTACATATTACAAATGGCTTCTTTGGTTTTCTCTTTCTCTTTATTTCTTCACCTCTTATCTCATAACCAATGACCCACAAGACATACCAACAAAAACAAAAACCATTGTCTCAAATTCTAATTCCAACGTTGTTTCTCATAACTCATTAGATATGTTGAAAAATTTGAAGGTGTTTGTTTATGAGCTACCTTCAAAATACAACAATGATTGGCTTGTAAATGGGAGGTGTAAAACACATTTGTTTGCTTCAGAAGTTGCTATTCACACCGCATTGTTGAAAAGTGATGTGAGAACTTTTGACCCTTATGAAGCTGATTTCTTCTTTGTTCCGGTTTATGTTTCTTGCAACTTCAGCACTGTTAACGGTTTTCCGGCAATTGGACATGCCCGTGCTCTCATTTCCTCCGCGGTTGAACTCATCGCCACCGAATATCCATTTTGGAATCGAAGCAGAGGATCTGACCATGTTTTCGTCGCTTCTCATGATTTTGGTGCATGTTTCCATACCCTGGAGGATAAGGCAATGGGTGATGGAATACCACAAATATTGAAGAATTCAATTGTGTTGCAGACATTTGGGGTTGAATATGAACATCCTTGTCAAGATGTTGAGAATGTGGTTATACCGCCTTATGTCCCGCCGGAGAAAGTTTCGAGGACGCTTGAGAAAGCTCCGGTGAACGGACGGCGAGATATTTGGGCTTTCTTTAGAGGGAAAATGGAAGTTCATCCTAAAAATGTTAGTGGACGGTATTACAGCAAGCGAGTGCGAACCGAGATATGGAGGAAATTCAACAGCAATCGAAGATTTTATCTACAACGACATAGATTTTCAGGTTACCAAATGGAAATTGCACGATCGGTATTCTGTCTATGTCCACTAGGGTGGGCACCATGGAGTCCGAGGCTTGTAGAATCAGTTGCACTCGGCTGTGTGCCCGTTATAATTGCCGATGGAATACGTTTGCCATTTTCCTCTACTGTGAAATGGCCGGAAATATCACTCACGGTGGCAGAGAAAGACGTAGGAAAACTCGACAAAATTCTCAAGCGCGTGGCAGCGACGAACCTAACTCAAATTCAGAAGAATCTGTGGGACCCAAAAACAAGACAGGCCCTACTTTTCAACAGTGAGGTCCAAGAAGGGGATGCCACGTGGCAAGTATTATTTAGTCTAAGTCAGAAGCTTGGTAGGTCCTACCATAGGTCCATGGTTTCTAGCCAATTGTGA
Protein sequence

>Msa0218100

MKLMNKHNTKPQQDHHKTCFFFTYYKWLLWFSLSLYFFTSYLITNDPQDIPTKTKTIVSNSNSNVVSHNSLDMLKNLKVFVYELPSKYNNDWLVNGRCKTHLFASEVAIHTALLKSDVRTFDPYEADFFFVPVYVSCNFSTVNGFPAIGHARALISSAVELIATEYPFWNRSRGSDHVFVASHDFGACFHTLEDKAMGDGIPQILKNSIVLQTFGVEYEHPCQDVENVVIPPYVPPEKVSRTLEKAPVNGRRDIWAFFRGKMEVHPKNVSGRYYSKRVRTEIWRKFNSNRRFYLQRHRFSGYQMEIARSVFCLCPLGWAPWSPRLVESVALGCVPVIIADGIRLPFSSTVKWPEISLTVAEKDVGKLDKILKRVAATNLTQIQKNLWDPKTRQALLFNSEVQEGDATWQVLFSLSQKLGRSYHRSMVSSQL*