AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0258180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0258180 MtrunA17_Chr2g0320251 97.517 443 11 0 1 443 1 443 0.0 902
Msa0258180 MtrunA17_Chr4g0028201 73.961 457 99 3 5 443 7 461 0.0 677
Msa0258180 MtrunA17_Chr8g0385651 43.280 372 201 5 65 430 21 388 5.19e-104 315
Msa0258180 MtrunA17_Chr8g0385671 42.742 372 203 5 65 430 21 388 3.09e-103 313
Msa0258180 MtrunA17_Chr1g0179651 30.088 339 185 11 77 398 1 304 4.92e-38 141
Msa0258180 MtrunA17_Chr7g0265461 28.371 356 224 8 76 426 60 389 3.54e-36 137
Msa0258180 MtrunA17_Chr7g0265451 31.737 334 191 10 74 398 175 480 7.45e-36 138
Msa0258180 MtrunA17_Chr1g0173651 27.427 412 213 15 77 416 342 739 6.33e-31 126
Msa0258180 MtrunA17_Chr5g0411991 26.331 357 216 9 81 426 86 406 1.25e-29 119
Msa0258180 MtrunA17_Chr3g0131051 26.911 327 216 6 74 398 151 456 3.58e-29 119
Msa0258180 MtrunA17_Chr4g0055991 31.164 292 174 10 132 410 244 521 1.77e-28 117
Msa0258180 MtrunA17_Chr3g0137491 28.212 358 211 14 83 426 57 382 3.58e-26 109
Msa0258180 MtrunA17_Chr4g0064471 25.691 362 218 13 83 426 133 461 4.57e-26 110
Msa0258180 MtrunA17_Chr7g0253441 27.684 354 210 13 84 426 154 472 2.28e-25 108
Msa0258180 MtrunA17_Chr4g0064411 26.496 351 225 11 84 426 128 453 7.59e-25 106
Msa0258180 MtrunA17_Chr4g0039811 25.707 389 223 13 89 437 112 474 8.76e-25 106
Msa0258180 MtrunA17_Chr7g0265381 26.989 352 217 11 85 426 44 365 1.24e-24 104
Msa0258180 MtrunA17_Chr3g0120131 28.788 330 195 13 80 398 147 447 1.84e-23 102
Msa0258180 MtrunA17_Chr3g0119721 26.074 349 223 13 85 426 15 335 3.83e-23 100
Msa0258180 MtrunA17_Chr3g0140131 25.641 351 223 10 85 426 264 585 9.07e-22 98.2
Msa0258180 MtrunA17_Chr4g0064461 26.016 369 226 14 74 426 119 456 9.47e-22 97.4
Msa0258180 MtrunA17_Chr8g0390121 27.798 277 156 8 134 395 325 572 1.06e-20 94.7
Msa0258180 MtrunA17_Chr1g0197381 27.152 302 181 11 134 426 268 539 1.33e-19 91.3
Msa0258180 MtrunA17_Chr3g0140141 24.759 311 185 11 132 426 266 543 2.13e-18 87.8
Msa0258180 MtrunA17_Chr6g0460631 25.758 396 224 16 89 438 113 484 2.43e-18 87.4
Msa0258180 MtrunA17_Chr1g0146181 25.155 322 177 11 85 383 257 537 7.06e-18 86.3
Msa0258180 MtrunA17_Chr8g0377331 24.419 344 206 10 89 398 49 372 1.71e-16 81.3
Msa0258180 MtrunA17_Chr3g0122631 24.812 399 230 15 42 398 4 374 3.25e-16 80.5
Msa0258180 MtrunA17_Chr1g0200871 28.772 285 141 12 131 378 139 398 3.11e-13 71.6
Msa0258180 MtrunA17_Chr3g0092341 24.351 308 192 11 130 421 186 468 4.31e-13 70.9
Msa0258180 MtrunA17_Chr5g0421571 31.206 141 84 4 81 221 139 266 2.32e-12 67.8
Msa0258180 MtrunA17_Chr7g0254391 23.171 410 247 15 63 430 91 474 4.53e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0258180 AT2G28110.1 63.657 443 130 3 25 437 3 444 0.0 591
Msa0258180 AT5G22940.1 65.144 416 129 3 43 442 49 464 0.0 576
Msa0258180 AT5G22940.2 61.348 445 127 4 43 442 49 493 0.0 561
Msa0258180 AT1G27440.1 42.192 365 203 5 67 430 27 384 7.21e-101 307
Msa0258180 AT5G61840.1 43.052 367 197 6 67 430 30 387 9.30e-101 307
Msa0258180 AT4G38040.1 26.966 356 215 12 81 426 96 416 1.11e-30 123
Msa0258180 AT4G16745.1 31.419 296 168 9 132 410 240 517 3.92e-30 123
Msa0258180 AT4G16745.2 31.544 298 169 9 132 412 240 519 7.26e-30 122
Msa0258180 AT4G38040.2 27.439 328 195 11 81 398 96 390 1.45e-29 119
Msa0258180 AT5G33290.1 26.579 380 231 13 66 426 139 489 1.85e-28 117
Msa0258180 AT5G20260.3 26.722 363 220 13 78 426 21 351 5.30e-28 114
Msa0258180 AT3G57630.2 26.157 432 225 15 72 425 329 744 1.03e-27 117
Msa0258180 AT3G57630.3 26.157 432 225 15 72 425 331 746 1.08e-27 116
Msa0258180 AT3G57630.1 26.157 432 225 15 72 425 331 746 1.08e-27 116
Msa0258180 AT5G11130.1 26.289 388 226 14 64 426 115 467 2.72e-27 114
Msa0258180 AT5G20260.1 26.966 356 214 13 85 426 1 324 3.46e-27 111
Msa0258180 AT5G20260.2 26.431 367 224 13 74 426 122 456 3.99e-27 113
Msa0258180 AT5G11130.2 26.885 305 194 8 128 426 42 323 4.96e-27 111
Msa0258180 AT5G03795.1 27.467 375 207 11 74 426 175 506 1.54e-26 112
Msa0258180 AT3G42180.1 25.337 371 229 11 74 426 119 459 5.87e-26 110
Msa0258180 AT5G25310.1 26.243 362 231 11 74 426 136 470 9.76e-26 109
Msa0258180 AT3G42180.4 25.556 360 220 11 85 426 1 330 2.63e-25 106
Msa0258180 AT5G16890.1 26.020 392 218 16 89 437 118 480 3.89e-23 102
Msa0258180 AT5G37000.1 26.101 318 203 9 85 395 222 514 4.70e-23 102
Msa0258180 AT5G11610.1 28.144 334 181 13 85 398 216 510 2.18e-22 100
Msa0258180 AT3G07620.1 25.926 351 226 12 83 426 126 449 2.73e-22 99.4
Msa0258180 AT5G19670.1 26.462 359 211 13 85 426 277 599 1.87e-21 97.4
Msa0258180 AT5G19670.2 26.462 359 211 13 85 426 277 599 1.87e-21 97.4
Msa0258180 AT4G32790.1 26.632 383 232 14 58 426 238 585 5.84e-21 95.9
Msa0258180 AT4G32790.3 26.632 383 232 14 58 426 238 585 5.84e-21 95.9
Msa0258180 AT4G32790.2 26.632 383 232 14 58 426 238 585 5.84e-21 95.9
Msa0258180 AT5G25820.1 27.042 355 216 15 85 426 320 644 1.99e-20 94.7
Msa0258180 AT1G67410.1 26.515 396 220 15 44 399 9 373 8.27e-18 85.5
Msa0258180 AT1G74680.1 26.494 385 217 15 89 430 75 436 8.13e-16 79.7
Msa0258180 AT5G44930.3 22.794 408 253 12 20 396 1 377 8.57e-16 79.3
Msa0258180 AT5G44930.2 22.963 405 256 11 20 396 1 377 1.16e-15 79.3
Msa0258180 AT5G44930.1 22.963 405 256 11 20 396 1 377 1.16e-15 79.3
Msa0258180 AT3G45400.1 26.797 306 188 11 131 423 154 436 3.11e-15 78.2
Msa0258180 AT3G03650.1 24.768 323 206 10 114 423 176 474 2.95e-14 75.1

Find 153 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTTGGGTATGGGAATT+TGG 0.110453 2_2:-54376033 Msa0258180:three_prime_UTR
AACAATGATTGGCTTGTAAA+TGG 0.183723 2_2:-54378346 Msa0258180:CDS
CATATTACAAATGGCTTCTT+TGG 0.197461 2_2:-54378531 Msa0258180:CDS
TTCCCATTCCCTTCAATAAT+TGG 0.205193 2_2:-54378889 None:intergenic
TACACGTGGTGGAATGTTAT+TGG 0.205370 2_2:-54376367 Msa0258180:three_prime_UTR
TGAACGGACGGCGAGATATT+TGG 0.213752 2_2:-54377657 Msa0258180:CDS
TCTCCACCGAATATCCATTT+TGG 0.224514 2_2:-54378126 Msa0258180:CDS
GCCACGTGGCATCCCCTTCT+TGG 0.225858 2_2:+54376464 None:intergenic
GAGATTGAGGTTTGGGTTAT+TGG 0.237000 2_2:+54378703 None:intergenic
GGCTTTCTTTAGAGGGAAAA+TGG 0.259284 2_2:-54377635 Msa0258180:CDS
CAATTGTGTTGCAGACATTT+GGG 0.271293 2_2:-54377777 Msa0258180:CDS
GATATTTGGGCTTTCTTTAG+AGG 0.303718 2_2:-54377643 Msa0258180:CDS
TTCGTCGCTTCTCATGATTT+TGG 0.305883 2_2:-54378076 Msa0258180:CDS
TTTAATTTGGTACTTGAAGT+TGG 0.308446 2_2:-54376047 Msa0258180:three_prime_UTR
TCCCATTCCCTTCAATAATT+GGG 0.316760 2_2:-54378888 None:intergenic
TCAATTGTGTTGCAGACATT+TGG 0.318671 2_2:-54377778 Msa0258180:CDS
ATATCTACTCTATGTCTCAT+AGG 0.321852 2_2:+54376206 None:intergenic
AGTAGTTGAGATTGAGGTTT+GGG 0.326601 2_2:+54378696 None:intergenic
ATTACAGCTAGGAATTTCTT+TGG 0.339615 2_2:-54376267 Msa0258180:three_prime_UTR
CCTCTGCTTCGATTCCAAAA+TGG 0.341711 2_2:+54378112 None:intergenic
TGTTGTGTCTTGTGGGTCAT+TGG 0.347100 2_2:+54378468 None:intergenic
GAACGGACGGCGAGATATTT+GGG 0.348869 2_2:-54377656 Msa0258180:CDS
GATTCTTCTGAATTTGAGTT+AGG 0.355459 2_2:+54376533 None:intergenic
ATTGAATTCTTCAATATTTG+TGG 0.363156 2_2:+54377796 None:intergenic
ACACGTGGTGGAATGTTATT+GGG 0.365435 2_2:-54376366 Msa0258180:three_prime_UTR
CTTCAAAATACAACAATGAT+TGG 0.372602 2_2:-54378357 Msa0258180:CDS
GAAGTTGGGTATGGGAATTT+GGG 0.386150 2_2:-54376032 Msa0258180:three_prime_UTR
ACCACGTGTAATTTCACAAT+TGG 0.386592 2_2:+54376380 None:intergenic
TTTGTTTGTGAAGGAGGATA+AGG 0.389184 2_2:-54377836 Msa0258180:intron
ATAGAATCCTTTGTTCTTGA+GGG 0.392954 2_2:+54378627 None:intergenic
CAATGTTTGTTGTGTCTTGT+GGG 0.395264 2_2:+54378461 None:intergenic
CCGATCGTGCAATTTCCATT+TGG 0.397921 2_2:+54376761 None:intergenic
TCGATTCCAAAATGGATATT+CGG 0.399390 2_2:+54378120 None:intergenic
TGCAACTTCAGCACGGTTAA+CGG 0.399763 2_2:-54378202 Msa0258180:CDS
GGCGAGTGCGAACCGAGATA+TGG 0.400049 2_2:-54376840 Msa0258180:CDS
TTAATTTGGTACTTGAAGTT+GGG 0.401304 2_2:-54376046 Msa0258180:three_prime_UTR
AGAGGAAGGTATGAGTTTGT+TGG 0.402814 2_2:+54378768 None:intergenic
GCCACCGTGAGTGATATTTC+CGG 0.405716 2_2:+54376610 None:intergenic
TTCCCTTCAATAATTGGGTT+GGG 0.405987 2_2:-54378883 None:intergenic
CATTTCACAGTAGAGGAAAA+TGG 0.406466 2_2:+54376634 None:intergenic
TTGGTACTTGAAGTTGGGTA+TGG 0.409813 2_2:-54376041 Msa0258180:three_prime_UTR
AAGAAGAAATCAGCTTCATA+AGG 0.417900 2_2:+54378244 None:intergenic
GAGGAAGGTATGAGTTTGTT+GGG 0.420304 2_2:+54378769 None:intergenic
TTGAAATGATTGATGAATAT+AGG 0.429858 2_2:-54376007 Msa0258180:three_prime_UTR
AAACCCTTCTCTTCAAATGT+TGG 0.434983 2_2:-54378662 Msa0258180:exon
GGGTCCATTGATGAGGTGGC+AGG 0.440189 2_2:-54376160 Msa0258180:three_prime_UTR
TATTCCATCGGCAATTATAA+CGG 0.440851 2_2:+54376663 None:intergenic
GGCATTTATTGCTCAGAAGA+AGG 0.442653 2_2:-54376246 Msa0258180:three_prime_UTR
CATAGAATCCTTTGTTCTTG+AGG 0.451453 2_2:+54378626 None:intergenic
ACAATGTTTGTTGTGTCTTG+TGG 0.455497 2_2:+54378460 None:intergenic
CTACGTGGATGCGTTGGCAT+TGG 0.458114 2_2:-54376310 Msa0258180:three_prime_UTR
TTAAATTACTCACCAGGGTA+TGG 0.462671 2_2:+54378044 None:intergenic
GAGTAGTTGAGATTGAGGTT+TGG 0.468390 2_2:+54378695 None:intergenic
TGAATGAGTAGTTGAGATTG+AGG 0.475097 2_2:+54378690 None:intergenic
ACAATGATTGGCTTGTAAAT+GGG 0.476562 2_2:-54378345 Msa0258180:CDS
GTTGAATTTCCTCCATATCT+CGG 0.479855 2_2:+54376828 None:intergenic
TCTCCCAACCCAATTATTGA+AGG 0.481646 2_2:+54378880 None:intergenic
GGACCTCACTGTTGAAAAGT+AGG 0.486436 2_2:+54376485 None:intergenic
TGGTACTTGAAGTTGGGTAT+GGG 0.487158 2_2:-54376040 Msa0258180:three_prime_UTR
ATTCCCTTCAATAATTGGGT+TGG 0.487440 2_2:-54378884 None:intergenic
ACTTTCTCCGGCGAGACATA+AGG 0.490904 2_2:+54377706 None:intergenic
GAGGTTTGGGTTATTGGTGT+AGG 0.491263 2_2:+54378709 None:intergenic
AAAACCTGCCACCTCATCAA+TGG 0.491703 2_2:+54376156 None:intergenic
ATTCCATCGGCAATTATAAC+GGG 0.492324 2_2:+54376664 None:intergenic
CATGAGAAGCGACGAAAACA+TGG 0.495556 2_2:+54378083 None:intergenic
AGTTCCAACATTTGAAGAGA+AGG 0.496102 2_2:+54378658 None:intergenic
TCTGTCTATGTCCACTAGGG+TGG 0.502220 2_2:-54376735 Msa0258180:CDS
TGAATATAGGAGAGAGATGT+GGG 0.502417 2_2:-54375994 Msa0258180:three_prime_UTR
TTCAACAGTGAGGTCCAAGA+AGG 0.503528 2_2:-54376478 Msa0258180:CDS
AGAAGAAATCAGCTTCATAA+GGG 0.512054 2_2:+54378245 None:intergenic
GTATTCTGTCTATGTCCACT+AGG 0.512595 2_2:-54376739 Msa0258180:CDS
TTCCGGCCATTTCACAGTAG+AGG 0.516050 2_2:+54376627 None:intergenic
AAGTTGCAAGAAACATAAAC+AGG 0.516113 2_2:+54378217 None:intergenic
TTCGGTGGAGACGAGATTAA+CGG 0.517380 2_2:+54378138 None:intergenic
GTTATTGGTGTAGGAAGAAG+TGG 0.517800 2_2:+54378718 None:intergenic
CTCCCAACCCAATTATTGAA+GGG 0.518457 2_2:+54378881 None:intergenic
TTCCTCTACTGTGAAATGGC+CGG 0.518492 2_2:-54376629 Msa0258180:CDS
CTCAAATTCAGAAGAATCTG+TGG 0.521949 2_2:-54376528 Msa0258180:CDS
GTTCCAACATTTGAAGAGAA+GGG 0.523681 2_2:+54378659 None:intergenic
ATATTTGGGCTTTCTTTAGA+GGG 0.526071 2_2:-54377642 Msa0258180:CDS
GGGTGGGTCCATTGATGAGG+TGG 0.526216 2_2:-54376164 Msa0258180:three_prime_UTR
CCAAATGGAAATTGCACGAT+CGG 0.526424 2_2:-54376761 Msa0258180:CDS
ATATCTCGCCGTCCGTTCAC+CGG 0.527175 2_2:+54377661 None:intergenic
GAAGAAGGAATTCTGATGGT+GGG 0.529622 2_2:-54376231 Msa0258180:three_prime_UTR
TATCTACTCTATGTCTCATA+GGG 0.532667 2_2:+54376207 None:intergenic
TCTACAAGCCTCGGACTCCA+TGG 0.543867 2_2:+54376709 None:intergenic
GTAGGAAGAAGTGGAAATGA+AGG 0.544837 2_2:+54378727 None:intergenic
TGGCGCATGTTTCCATACCC+TGG 0.550490 2_2:-54378056 Msa0258180:intron
TGGCAATGACAACTATAAAG+TGG 0.550975 2_2:-54376290 Msa0258180:three_prime_UTR
AGTTTCCTACGTGGATGCGT+TGG 0.554385 2_2:-54376316 Msa0258180:three_prime_UTR
CTGTCTATGTCCACTAGGGT+GGG 0.559699 2_2:-54376734 Msa0258180:CDS
TAGGTCCTACCGTAGGTCCA+TGG 0.561146 2_2:-54376410 Msa0258180:CDS
TTTAGTCTAAGTCAGAAGCT+TGG 0.565244 2_2:-54376433 Msa0258180:CDS
GCACGGGCATGTCCAATAGC+CGG 0.565290 2_2:+54378175 None:intergenic
TCAACAGTGAGGTCCAAGAA+GGG 0.566785 2_2:-54376477 Msa0258180:CDS
GACCTCACTGTTGAAAAGTA+GGG 0.567183 2_2:+54376486 None:intergenic
AAAACAAGGGAAGAAGAGGA+AGG 0.570113 2_2:+54378754 None:intergenic
GGTGGGTGGGTCCATTGATG+AGG 0.571396 2_2:-54376167 Msa0258180:three_prime_UTR
GGTGGGCACCATGGAGTCCG+AGG 0.573935 2_2:-54376717 Msa0258180:CDS
CTTGTAGAATCAGTTGCACT+CGG 0.574677 2_2:-54376694 Msa0258180:CDS
TCACAATTGGCTAGAAACCA+TGG 0.575572 2_2:+54376393 None:intergenic
GGCTAGAAACCATGGACCTA+CGG 0.575901 2_2:+54376401 None:intergenic
ATGAATATAGGAGAGAGATG+TGG 0.577671 2_2:-54375995 Msa0258180:three_prime_UTR
TAGAATCCTTTGTTCTTGAG+GGG 0.578623 2_2:+54378628 None:intergenic
CTTGAGAAAGCTCCGGTGAA+CGG 0.579154 2_2:-54377673 Msa0258180:CDS
GGCGGAGGAAATGAGAGCAC+GGG 0.580674 2_2:+54378159 None:intergenic
GCAACTGATTCTACAAGCCT+CGG 0.580697 2_2:+54376700 None:intergenic
AGAAAGCTCCGGTGAACGGA+CGG 0.583778 2_2:-54377669 Msa0258180:CDS
GTCCACTAGGGTGGGCACCA+TGG 0.587305 2_2:-54376726 Msa0258180:CDS
CTATAAAGTGGATTACAGCT+AGG 0.589040 2_2:-54376278 Msa0258180:three_prime_UTR
ATTCCAAAATGGATATTCGG+TGG 0.597749 2_2:+54378123 None:intergenic
AGCGTCCTCGAAACTTTCTC+CGG 0.598726 2_2:+54377694 None:intergenic
CAACAGTGAGGTCCAAGAAG+GGG 0.599472 2_2:-54376476 Msa0258180:CDS
GAGGACGCTTGAGAAAGCTC+CGG 0.603668 2_2:-54377680 Msa0258180:CDS
GTCTAAGTCAGAAGCTTGGT+AGG 0.604636 2_2:-54376429 Msa0258180:CDS
GAGTGCGAACCGAGATATGG+AGG 0.605750 2_2:-54376837 Msa0258180:CDS
GAGGATAAGGCAATGGACGA+TGG 0.606572 2_2:-54377823 Msa0258180:CDS
TATTCTGTCTATGTCCACTA+GGG 0.612391 2_2:-54376738 Msa0258180:CDS
AAATGGCAAACGTATTCCAT+CGG 0.615484 2_2:+54376651 None:intergenic
CTCAGAAGAAGGAATTCTGA+TGG 0.617316 2_2:-54376235 Msa0258180:three_prime_UTR
AGAAGAAGGAATTCTGATGG+TGG 0.617831 2_2:-54376232 Msa0258180:three_prime_UTR
CTCCATGGTGCCCACCCTAG+TGG 0.618009 2_2:+54376724 None:intergenic
CGGCGGAGGAAATGAGAGCA+CGG 0.619007 2_2:+54378158 None:intergenic
GGAGACGAGATTAACGGCGG+AGG 0.620953 2_2:+54378144 None:intergenic
AATTGTGAAATTACACGTGG+TGG 0.632003 2_2:-54376378 Msa0258180:exon
AAGACTCCCCTCAAGAACAA+AGG 0.632480 2_2:-54378634 Msa0258180:CDS
TGTTTCTTGCAACTTCAGCA+CGG 0.635379 2_2:-54378209 Msa0258180:CDS
TTGTCAAGATGTTGAGAATG+TGG 0.637717 2_2:-54377737 Msa0258180:CDS
AAGAAGGAATTCTGATGGTG+GGG 0.639354 2_2:-54376230 Msa0258180:three_prime_UTR
TCTCGCCGGAGAAAGTTTCG+AGG 0.644312 2_2:-54377699 Msa0258180:CDS
AATTGTGTTGCAGACATTTG+GGG 0.645114 2_2:-54377776 Msa0258180:CDS
TGTGAAGGAGGATAAGGCAA+TGG 0.645309 2_2:-54377830 Msa0258180:intron
GTCATTGGTGATGAGATAAG+AGG 0.648425 2_2:+54378483 None:intergenic
TCAAATTCAGAAGAATCTGT+GGG 0.650477 2_2:-54376527 Msa0258180:CDS
TCCAAGAAGGGGATGCCACG+TGG 0.655039 2_2:-54376465 Msa0258180:CDS
CGACAAAATTCTCAAGCGCG+TGG 0.655151 2_2:-54376566 Msa0258180:CDS
AGAAACCATGGACCTACGGT+AGG 0.657642 2_2:+54376405 None:intergenic
GTGCCCGTTATAATTGCCGA+TGG 0.661063 2_2:-54376667 Msa0258180:CDS
ATGGCCGGAAATATCACTCA+CGG 0.666857 2_2:-54376614 Msa0258180:CDS
AGAAGCGACGAAAACATGGT+CGG 0.680161 2_2:+54378087 None:intergenic
ACATTCTCAACATCTTGACA+AGG 0.692808 2_2:+54377739 None:intergenic
TGAGTTATGAGAAACAACGT+TGG 0.700940 2_2:+54378423 None:intergenic
TATACCGCCTTATGTCTCGC+CGG 0.705097 2_2:-54377713 Msa0258180:CDS
TTCTCCGGCGAGACATAAGG+CGG 0.706232 2_2:+54377709 None:intergenic
ATGATTGGCTTGTAAATGGG+AGG 0.708014 2_2:-54378342 Msa0258180:CDS
CAATGCCAACGCATCCACGT+AGG 0.713905 2_2:+54376311 None:intergenic
AGCTTGGTAGGTCCTACCGT+AGG 0.716431 2_2:-54376417 Msa0258180:CDS
GCCAATTGTGAAATTACACG+TGG 0.723864 2_2:-54376381 Msa0258180:exon
ATGAAGCTTAGTTTCCTACG+TGG 0.725233 2_2:-54376325 Msa0258180:three_prime_UTR
ACGGTGGCAGAGAAAGACGT+AGG 0.728097 2_2:-54376595 Msa0258180:CDS
ACTAAATAATACTTGCCACG+TGG 0.759736 2_2:+54376450 None:intergenic
GCCGGAAATATCACTCACGG+TGG 0.764283 2_2:-54376611 Msa0258180:CDS
GGTGGAGACGAGATTAACGG+CGG 0.770816 2_2:+54378141 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATATATTTTTGTATTTTT+AGG - chr2_2:54378626-54378645 Msa0258180:CDS 10.0%
!!! GATATATTTTTGTATTTTTA+GGG - chr2_2:54378627-54378646 Msa0258180:CDS 10.0%
!!! TCAATAAAGAAATTTTAATT+TGG - chr2_2:54378755-54378774 Msa0258180:five_prime_UTR 10.0%
!!! TAAGTTATAATTTTGTTAGA+GGG + chr2_2:54375988-54376007 None:intergenic 15.0%
!! AACAAAAATAAACAAATTGT+AGG + chr2_2:54376013-54376032 None:intergenic 15.0%
!!! TCTTTTTTACATATTACAAA+TGG - chr2_2:54376275-54376294 Msa0258180:three_prime_UTR 15.0%
!!! TTTTTACACGTTTTAAATTA+AGG - chr2_2:54377296-54377315 Msa0258180:intron 15.0%
!! TTTAACAGAAATTTAAATCA+TGG + chr2_2:54377350-54377369 None:intergenic 15.0%
!!! ATCTTTTGAATGTAATTATT+TGG - chr2_2:54377815-54377834 Msa0258180:CDS 15.0%
!!! ATTTTTAATGTGAAAACATA+TGG - chr2_2:54378867-54378886 Msa0258180:five_prime_UTR 15.0%
!!! TTTTTAATGTGAAAACATAT+GGG - chr2_2:54378868-54378887 Msa0258180:five_prime_UTR 15.0%
!!! GTAAGTTATAATTTTGTTAG+AGG + chr2_2:54375989-54376008 None:intergenic 20.0%
!! AAATGAAGGAAAAAAAACAA+GGG + chr2_2:54376077-54376096 None:intergenic 20.0%
!!! AAAAGAAACATGTTTTATGA+TGG + chr2_2:54376261-54376280 None:intergenic 20.0%
!! AGATATGTTGAAAAATTTGA+AGG - chr2_2:54376417-54376436 Msa0258180:CDS 20.0%
!! AAATTGAATGTTTGATATCA+CGG + chr2_2:54376920-54376939 None:intergenic 20.0%
!!! TTTGAATTTTTTGTTTGTGA+AGG - chr2_2:54376970-54376989 Msa0258180:intron 20.0%
!! ATTGAATTCTTCAATATTTG+TGG + chr2_2:54377022-54377041 None:intergenic 20.0%
!!! TAGTTAATGATTTTATTGCA+GGG + chr2_2:54377263-54377282 None:intergenic 20.0%
!!! TTAGTTAATGATTTTATTGC+AGG + chr2_2:54377264-54377283 None:intergenic 20.0%
!!! AGGTGATTTTAATCAAATTA+TGG - chr2_2:54377316-54377335 Msa0258180:intron 20.0%
!!! TTTAAGTAAAAGCTACATTT+TGG + chr2_2:54377476-54377495 None:intergenic 20.0%
!! ATTTCTTAAGATGTGTTTAT+AGG + chr2_2:54377565-54377584 None:intergenic 20.0%
!!! TTTTCGTACAAATATACATT+TGG - chr2_2:54377695-54377714 Msa0258180:CDS 20.0%
!! AATCTATTAAACTCATTGAA+TGG - chr2_2:54377730-54377749 Msa0258180:CDS 20.0%
!! CAATTACATTAATTCATCAA+CGG + chr2_2:54377934-54377953 None:intergenic 20.0%
!!! ATATTTTTGTATTTTTAGGG+TGG - chr2_2:54378630-54378649 Msa0258180:CDS 20.0%
!!! TATTTTTGTATTTTTAGGGT+GGG - chr2_2:54378631-54378650 Msa0258180:CDS 20.0%
!! TTGAAATGATTGATGAATAT+AGG - chr2_2:54378808-54378827 Msa0258180:five_prime_UTR 20.0%
! GAAATGAAGGAAAAAAAACA+AGG + chr2_2:54376078-54376097 None:intergenic 25.0%
!!! CAATCATTGTTGTATTTTGA+AGG + chr2_2:54376460-54376479 None:intergenic 25.0%
! CTTCAAAATACAACAATGAT+TGG - chr2_2:54376458-54376477 Msa0258180:CDS 25.0%
!!! ACTGTGTTTTTTATTTGTGA+AGG - chr2_2:54377954-54377973 Msa0258180:intron 25.0%
!!! TTTAATTTGGTACTTGAAGT+TGG - chr2_2:54378768-54378787 Msa0258180:five_prime_UTR 25.0%
!! TTAATTTGGTACTTGAAGTT+GGG - chr2_2:54378769-54378788 Msa0258180:five_prime_UTR 25.0%
AAAAAAAACAAGGGAAGAAG+AGG + chr2_2:54376068-54376087 None:intergenic 30.0%
ATAGAATCCTTTGTTCTTGA+GGG + chr2_2:54376191-54376210 None:intergenic 30.0%
CATATTACAAATGGCTTCTT+TGG - chr2_2:54376284-54376303 Msa0258180:three_prime_UTR 30.0%
!! AACAATGATTGGCTTGTAAA+TGG - chr2_2:54376469-54376488 Msa0258180:CDS 30.0%
!! ACAATGATTGGCTTGTAAAT+GGG - chr2_2:54376470-54376489 Msa0258180:CDS 30.0%
AGAAGAAATCAGCTTCATAA+GGG + chr2_2:54376573-54376592 None:intergenic 30.0%
AAGAAGAAATCAGCTTCATA+AGG + chr2_2:54376574-54376593 None:intergenic 30.0%
AAGTTGCAAGAAACATAAAC+AGG + chr2_2:54376601-54376620 None:intergenic 30.0%
TCGATTCCAAAATGGATATT+CGG + chr2_2:54376698-54376717 None:intergenic 30.0%
TGATGTTAAATTACTCACCA+GGG + chr2_2:54376779-54376798 None:intergenic 30.0%
ATGATGTTAAATTACTCACC+AGG + chr2_2:54376780-54376799 None:intergenic 30.0%
! GTAGTTTTTGAATGTCAACA+TGG - chr2_2:54376870-54376889 Msa0258180:intron 30.0%
!!! GAATTTTTTGTTTGTGAAGG+AGG - chr2_2:54376973-54376992 Msa0258180:intron 30.0%
ATATTTGGGCTTTCTTTAGA+GGG - chr2_2:54377173-54377192 Msa0258180:intron 30.0%
GTTCATCCTAAAAATGTTAG+TGG - chr2_2:54377205-54377224 Msa0258180:intron 30.0%
ATCCTAAAAATGTTAGTGGA+CGG - chr2_2:54377209-54377228 Msa0258180:intron 30.0%
CTACCTTTGTGAATTGATAT+GGG - chr2_2:54377874-54377893 Msa0258180:intron 30.0%
TAACCCATATCAATTCACAA+AGG + chr2_2:54377880-54377899 None:intergenic 30.0%
! ATTCATTCTGCATTGCATTT+TGG - chr2_2:54377905-54377924 Msa0258180:intron 30.0%
!!! TAGATTTTCAGGTTACCAAA+TGG - chr2_2:54378039-54378058 Msa0258180:intron 30.0%
TATTCCATCGGCAATTATAA+CGG + chr2_2:54378155-54378174 None:intergenic 30.0%
! GATTCTTCTGAATTTGAGTT+AGG + chr2_2:54378285-54378304 None:intergenic 30.0%
TCAAATTCAGAAGAATCTGT+GGG - chr2_2:54378288-54378307 Msa0258180:CDS 30.0%
ATTACAGCTAGGAATTTCTT+TGG - chr2_2:54378548-54378567 Msa0258180:CDS 30.0%
TATCTACTCTATGTCTCATA+GGG + chr2_2:54378611-54378630 None:intergenic 30.0%
ATATCTACTCTATGTCTCAT+AGG + chr2_2:54378612-54378631 None:intergenic 30.0%
AGTAGTTGAGATTGAGGTTT+GGG + chr2_2:54376122-54376141 None:intergenic 35.0%
TGAATGAGTAGTTGAGATTG+AGG + chr2_2:54376128-54376147 None:intergenic 35.0%
AAACCCTTCTCTTCAAATGT+TGG - chr2_2:54376153-54376172 Msa0258180:three_prime_UTR 35.0%
GTTCCAACATTTGAAGAGAA+GGG + chr2_2:54376159-54376178 None:intergenic 35.0%
AGTTCCAACATTTGAAGAGA+AGG + chr2_2:54376160-54376179 None:intergenic 35.0%
TAGAATCCTTTGTTCTTGAG+GGG + chr2_2:54376190-54376209 None:intergenic 35.0%
CATAGAATCCTTTGTTCTTG+AGG + chr2_2:54376192-54376211 None:intergenic 35.0%
!!! GTTTTATGATGGTCTTGTTG+AGG + chr2_2:54376250-54376269 None:intergenic 35.0%
!! CAATGTTTGTTGTGTCTTGT+GGG + chr2_2:54376357-54376376 None:intergenic 35.0%
!! ACAATGTTTGTTGTGTCTTG+TGG + chr2_2:54376358-54376377 None:intergenic 35.0%
TGAGTTATGAGAAACAACGT+TGG + chr2_2:54376395-54376414 None:intergenic 35.0%
!! CACATCACTTTTCAACAATG+CGG + chr2_2:54376539-54376558 None:intergenic 35.0%
ATTCCAAAATGGATATTCGG+TGG + chr2_2:54376695-54376714 None:intergenic 35.0%
TTAAATTACTCACCAGGGTA+TGG + chr2_2:54376774-54376793 None:intergenic 35.0%
TTTGTTTGTGAAGGAGGATA+AGG - chr2_2:54376979-54376998 Msa0258180:intron 35.0%
TCAATTGTGTTGCAGACATT+TGG - chr2_2:54377037-54377056 Msa0258180:intron 35.0%
CAATTGTGTTGCAGACATTT+GGG - chr2_2:54377038-54377057 Msa0258180:intron 35.0%
AATTGTGTTGCAGACATTTG+GGG - chr2_2:54377039-54377058 Msa0258180:intron 35.0%
ACATTCTCAACATCTTGACA+AGG + chr2_2:54377079-54377098 None:intergenic 35.0%
TTGTCAAGATGTTGAGAATG+TGG - chr2_2:54377078-54377097 Msa0258180:intron 35.0%
GATATTTGGGCTTTCTTTAG+AGG - chr2_2:54377172-54377191 Msa0258180:intron 35.0%
! TACCGTCCACTAACATTTTT+AGG + chr2_2:54377214-54377233 None:intergenic 35.0%
! TTGTGTTATTAACACGTGCA+TGG - chr2_2:54377660-54377679 Msa0258180:CDS 35.0%
ATGCTATCTCAATCAAAGTG+AGG + chr2_2:54377791-54377810 None:intergenic 35.0%
GCTACCTTTGTGAATTGATA+TGG - chr2_2:54377873-54377892 Msa0258180:intron 35.0%
GTTGAATTTCCTCCATATCT+CGG + chr2_2:54377990-54378009 None:intergenic 35.0%
! CTACAACGACATAGATTTTC+AGG - chr2_2:54378028-54378047 Msa0258180:intron 35.0%
TATTCTGTCTATGTCCACTA+GGG - chr2_2:54378077-54378096 Msa0258180:CDS 35.0%
ATTCCATCGGCAATTATAAC+GGG + chr2_2:54378154-54378173 None:intergenic 35.0%
AAATGGCAAACGTATTCCAT+CGG + chr2_2:54378167-54378186 None:intergenic 35.0%
CATTTCACAGTAGAGGAAAA+TGG + chr2_2:54378184-54378203 None:intergenic 35.0%
! CATTTTCCTCTACTGTGAAA+TGG - chr2_2:54378182-54378201 Msa0258180:CDS 35.0%
CTCAAATTCAGAAGAATCTG+TGG - chr2_2:54378287-54378306 Msa0258180:CDS 35.0%
ACTAAATAATACTTGCCACG+TGG + chr2_2:54378368-54378387 None:intergenic 35.0%
TTTAGTCTAAGTCAGAAGCT+TGG - chr2_2:54378382-54378401 Msa0258180:CDS 35.0%
ACCACGTGTAATTTCACAAT+TGG + chr2_2:54378438-54378457 None:intergenic 35.0%
AATTGTGAAATTACACGTGG+TGG - chr2_2:54378437-54378456 Msa0258180:CDS 35.0%
TGGCAATGACAACTATAAAG+TGG - chr2_2:54378525-54378544 Msa0258180:CDS 35.0%
!! CTATAAAGTGGATTACAGCT+AGG - chr2_2:54378537-54378556 Msa0258180:CDS 35.0%
!!! TTTTGTATTTTTAGGGTGGG+TGG - chr2_2:54378634-54378653 Msa0258180:CDS 35.0%
!!! TTTGTATTTTTAGGGTGGGT+GGG - chr2_2:54378635-54378654 Msa0258180:CDS 35.0%
ATGAATATAGGAGAGAGATG+TGG - chr2_2:54378820-54378839 Msa0258180:five_prime_UTR 35.0%
TGAATATAGGAGAGAGATGT+GGG - chr2_2:54378821-54378840 Msa0258180:five_prime_UTR 35.0%
!! AGAGGGTTTTGTGTTGTGTT+GGG + chr2_2:54375971-54375990 None:intergenic 40.0%
!! TAGAGGGTTTTGTGTTGTGT+TGG + chr2_2:54375972-54375991 None:intergenic 40.0%
! GAGGAAGGTATGAGTTTGTT+GGG + chr2_2:54376049-54376068 None:intergenic 40.0%
! AGAGGAAGGTATGAGTTTGT+TGG + chr2_2:54376050-54376069 None:intergenic 40.0%
AAAACAAGGGAAGAAGAGGA+AGG + chr2_2:54376064-54376083 None:intergenic 40.0%
GTAGGAAGAAGTGGAAATGA+AGG + chr2_2:54376091-54376110 None:intergenic 40.0%
GTTATTGGTGTAGGAAGAAG+TGG + chr2_2:54376100-54376119 None:intergenic 40.0%
! GAGATTGAGGTTTGGGTTAT+TGG + chr2_2:54376115-54376134 None:intergenic 40.0%
GAGTAGTTGAGATTGAGGTT+TGG + chr2_2:54376123-54376142 None:intergenic 40.0%
!! GTCATTGGTGATGAGATAAG+AGG + chr2_2:54376335-54376354 None:intergenic 40.0%
!! ATGATTGGCTTGTAAATGGG+AGG - chr2_2:54376473-54376492 Msa0258180:CDS 40.0%
TGTTTCTTGCAACTTCAGCA+CGG - chr2_2:54376606-54376625 Msa0258180:CDS 40.0%
! TCTCCACCGAATATCCATTT+TGG - chr2_2:54376689-54376708 Msa0258180:CDS 40.0%
! TTCGTCGCTTCTCATGATTT+TGG - chr2_2:54376739-54376758 Msa0258180:CDS 40.0%
! GGCTTTCTTTAGAGGGAAAA+TGG - chr2_2:54377180-54377199 Msa0258180:intron 40.0%
CCAAATGGAAATTGCACGAT+CGG - chr2_2:54378054-54378073 Msa0258180:intron 40.0%
GTATTCTGTCTATGTCCACT+AGG - chr2_2:54378076-54378095 Msa0258180:CDS 40.0%
CTTGTAGAATCAGTTGCACT+CGG - chr2_2:54378121-54378140 Msa0258180:CDS 40.0%
GACCTCACTGTTGAAAAGTA+GGG + chr2_2:54378332-54378351 None:intergenic 40.0%
TCACAATTGGCTAGAAACCA+TGG + chr2_2:54378425-54378444 None:intergenic 40.0%
GCCAATTGTGAAATTACACG+TGG - chr2_2:54378434-54378453 Msa0258180:CDS 40.0%
! TACACGTGGTGGAATGTTAT+TGG - chr2_2:54378448-54378467 Msa0258180:CDS 40.0%
! ACACGTGGTGGAATGTTATT+GGG - chr2_2:54378449-54378468 Msa0258180:CDS 40.0%
ATGAAGCTTAGTTTCCTACG+TGG - chr2_2:54378490-54378509 Msa0258180:CDS 40.0%
GGCATTTATTGCTCAGAAGA+AGG - chr2_2:54378569-54378588 Msa0258180:CDS 40.0%
CTCAGAAGAAGGAATTCTGA+TGG - chr2_2:54378580-54378599 Msa0258180:CDS 40.0%
AGAAGAAGGAATTCTGATGG+TGG - chr2_2:54378583-54378602 Msa0258180:CDS 40.0%
GAAGAAGGAATTCTGATGGT+GGG - chr2_2:54378584-54378603 Msa0258180:CDS 40.0%
! AAGAAGGAATTCTGATGGTG+GGG - chr2_2:54378585-54378604 Msa0258180:CDS 40.0%
TTGGTACTTGAAGTTGGGTA+TGG - chr2_2:54378774-54378793 Msa0258180:five_prime_UTR 40.0%
TGGTACTTGAAGTTGGGTAT+GGG - chr2_2:54378775-54378794 Msa0258180:five_prime_UTR 40.0%
TGAAGTTGGGTATGGGAATT+TGG - chr2_2:54378782-54378801 Msa0258180:five_prime_UTR 40.0%
GAAGTTGGGTATGGGAATTT+GGG - chr2_2:54378783-54378802 Msa0258180:five_prime_UTR 40.0%
! GAGGTTTGGGTTATTGGTGT+AGG + chr2_2:54376109-54376128 None:intergenic 45.0%
AAGACTCCCCTCAAGAACAA+AGG - chr2_2:54376181-54376200 Msa0258180:three_prime_UTR 45.0%
TGTTGTGTCTTGTGGGTCAT+TGG + chr2_2:54376350-54376369 None:intergenic 45.0%
TGCAACTTCAGCACGGTTAA+CGG - chr2_2:54376613-54376632 Msa0258180:CDS 45.0%
! GTTAACGGTTTTCCGGCTAT+TGG - chr2_2:54376628-54376647 Msa0258180:CDS 45.0%
TTCGGTGGAGACGAGATTAA+CGG + chr2_2:54376680-54376699 None:intergenic 45.0%
CCTCTGCTTCGATTCCAAAA+TGG + chr2_2:54376706-54376725 None:intergenic 45.0%
!!! CCATTTTGGAATCGAAGCAG+AGG - chr2_2:54376703-54376722 Msa0258180:CDS 45.0%
AGAAGCGACGAAAACATGGT+CGG + chr2_2:54376731-54376750 None:intergenic 45.0%
CATGAGAAGCGACGAAAACA+TGG + chr2_2:54376735-54376754 None:intergenic 45.0%
TGTGAAGGAGGATAAGGCAA+TGG - chr2_2:54376985-54377004 Msa0258180:intron 45.0%
CCGATCGTGCAATTTCCATT+TGG + chr2_2:54378057-54378076 None:intergenic 45.0%
! GCAACTGATTCTACAAGCCT+CGG + chr2_2:54378118-54378137 None:intergenic 45.0%
TTCCTCTACTGTGAAATGGC+CGG - chr2_2:54378186-54378205 Msa0258180:CDS 45.0%
ATGGCCGGAAATATCACTCA+CGG - chr2_2:54378201-54378220 Msa0258180:CDS 45.0%
!!! GTAGGGCCTGTCTTGTTTTT+GGG + chr2_2:54378315-54378334 None:intergenic 45.0%
!!! AGTAGGGCCTGTCTTGTTTT+TGG + chr2_2:54378316-54378335 None:intergenic 45.0%
GGACCTCACTGTTGAAAAGT+AGG + chr2_2:54378333-54378352 None:intergenic 45.0%
TTCAACAGTGAGGTCCAAGA+AGG - chr2_2:54378337-54378356 Msa0258180:CDS 45.0%
TCAACAGTGAGGTCCAAGAA+GGG - chr2_2:54378338-54378357 Msa0258180:CDS 45.0%
GTCTAAGTCAGAAGCTTGGT+AGG - chr2_2:54378386-54378405 Msa0258180:CDS 45.0%
AAAACCTGCCACCTCATCAA+TGG + chr2_2:54378662-54378681 None:intergenic 45.0%
!!! TGAGGTGGCAGGTTTTGATT+TGG - chr2_2:54378666-54378685 Msa0258180:exon 45.0%
!! AAAAATATATATTAAATTCA+TGG + chr2_2:54377518-54377537 None:intergenic 5.0%
! CAGCACGGTTAACGGTTTTC+CGG - chr2_2:54376621-54376640 Msa0258180:CDS 50.0%
GAGGATAAGGCAATGGACGA+TGG - chr2_2:54376992-54377011 Msa0258180:intron 50.0%
TATACCGCCTTATGTCTCGC+CGG - chr2_2:54377102-54377121 Msa0258180:intron 50.0%
ACTTTCTCCGGCGAGACATA+AGG + chr2_2:54377112-54377131 None:intergenic 50.0%
AGCGTCCTCGAAACTTTCTC+CGG + chr2_2:54377124-54377143 None:intergenic 50.0%
CTTGAGAAAGCTCCGGTGAA+CGG - chr2_2:54377142-54377161 Msa0258180:intron 50.0%
TGAACGGACGGCGAGATATT+TGG - chr2_2:54377158-54377177 Msa0258180:intron 50.0%
GAACGGACGGCGAGATATTT+GGG - chr2_2:54377159-54377178 Msa0258180:intron 50.0%
TCTGTCTATGTCCACTAGGG+TGG - chr2_2:54378080-54378099 Msa0258180:CDS 50.0%
CTGTCTATGTCCACTAGGGT+GGG - chr2_2:54378081-54378100 Msa0258180:CDS 50.0%
GTGCCCGTTATAATTGCCGA+TGG - chr2_2:54378148-54378167 Msa0258180:CDS 50.0%
TTCCGGCCATTTCACAGTAG+AGG + chr2_2:54378191-54378210 None:intergenic 50.0%
GCCACCGTGAGTGATATTTC+CGG + chr2_2:54378208-54378227 None:intergenic 50.0%
CGACAAAATTCTCAAGCGCG+TGG - chr2_2:54378249-54378268 Msa0258180:CDS 50.0%
GTGGGACCCAAAAACAAGAC+AGG - chr2_2:54378306-54378325 Msa0258180:CDS 50.0%
!! GGCCCTACTTTTCAACAGTG+AGG - chr2_2:54378327-54378346 Msa0258180:CDS 50.0%
CAACAGTGAGGTCCAAGAAG+GGG - chr2_2:54378339-54378358 Msa0258180:CDS 50.0%
AGAAACCATGGACCTACGGT+AGG + chr2_2:54378413-54378432 None:intergenic 50.0%
GGCTAGAAACCATGGACCTA+CGG + chr2_2:54378417-54378436 None:intergenic 50.0%
AGTTTCCTACGTGGATGCGT+TGG - chr2_2:54378499-54378518 Msa0258180:CDS 50.0%
GGTGGAGACGAGATTAACGG+CGG + chr2_2:54376677-54376696 None:intergenic 55.0%
TGGCGCATGTTTCCATACCC+TGG - chr2_2:54376759-54376778 Msa0258180:CDS 55.0%
TTCTCCGGCGAGACATAAGG+CGG + chr2_2:54377109-54377128 None:intergenic 55.0%
TCTCGCCGGAGAAAGTTTCG+AGG - chr2_2:54377116-54377135 Msa0258180:intron 55.0%
GAGGACGCTTGAGAAAGCTC+CGG - chr2_2:54377135-54377154 Msa0258180:intron 55.0%
AGAAAGCTCCGGTGAACGGA+CGG - chr2_2:54377146-54377165 Msa0258180:intron 55.0%
ATATCTCGCCGTCCGTTCAC+CGG + chr2_2:54377157-54377176 None:intergenic 55.0%
GAGTGCGAACCGAGATATGG+AGG - chr2_2:54377978-54377997 Msa0258180:intron 55.0%
TCTACAAGCCTCGGACTCCA+TGG + chr2_2:54378109-54378128 None:intergenic 55.0%
GCCGGAAATATCACTCACGG+TGG - chr2_2:54378204-54378223 Msa0258180:CDS 55.0%
ACGGTGGCAGAGAAAGACGT+AGG - chr2_2:54378220-54378239 Msa0258180:CDS 55.0%
AGCTTGGTAGGTCCTACCGT+AGG - chr2_2:54378398-54378417 Msa0258180:CDS 55.0%
TAGGTCCTACCGTAGGTCCA+TGG - chr2_2:54378405-54378424 Msa0258180:CDS 55.0%
CAATGCCAACGCATCCACGT+AGG + chr2_2:54378507-54378526 None:intergenic 55.0%
! CTACGTGGATGCGTTGGCAT+TGG - chr2_2:54378505-54378524 Msa0258180:CDS 55.0%
GCACGGGCATGTCCAATAGC+CGG + chr2_2:54376643-54376662 None:intergenic 60.0%
! GGCGGAGGAAATGAGAGCAC+GGG + chr2_2:54376659-54376678 None:intergenic 60.0%
CGGCGGAGGAAATGAGAGCA+CGG + chr2_2:54376660-54376679 None:intergenic 60.0%
GGAGACGAGATTAACGGCGG+AGG + chr2_2:54376674-54376693 None:intergenic 60.0%
GGCGAGTGCGAACCGAGATA+TGG - chr2_2:54377975-54377994 Msa0258180:intron 60.0%
TCCAAGAAGGGGATGCCACG+TGG - chr2_2:54378350-54378369 Msa0258180:CDS 60.0%
GGTGGGTGGGTCCATTGATG+AGG - chr2_2:54378648-54378667 Msa0258180:CDS 60.0%
GGGTGGGTCCATTGATGAGG+TGG - chr2_2:54378651-54378670 Msa0258180:exon 60.0%
GGGTCCATTGATGAGGTGGC+AGG - chr2_2:54378655-54378674 Msa0258180:exon 60.0%
GTCCACTAGGGTGGGCACCA+TGG - chr2_2:54378089-54378108 Msa0258180:CDS 65.0%
CTCCATGGTGCCCACCCTAG+TGG + chr2_2:54378094-54378113 None:intergenic 65.0%
GCCACGTGGCATCCCCTTCT+TGG + chr2_2:54378354-54378373 None:intergenic 65.0%
GGTGGGCACCATGGAGTCCG+AGG - chr2_2:54378098-54378117 Msa0258180:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 54375937 54378900 54375937 ID=Msa0258180;Name=Msa0258180
chr2_2 mRNA 54375937 54378900 54375937 ID=Msa0258180-mRNA-1;Parent=Msa0258180;Name=Msa0258180-mRNA-1;_AED=0.00;_eAED=0.00;_QI=232|1|1|1|1|1|3|456|443
chr2_2 exon 54375937 54376861 54375937 ID=Msa0258180-mRNA-1:exon:16313;Parent=Msa0258180-mRNA-1
chr2_2 exon 54377595 54377845 54377595 ID=Msa0258180-mRNA-1:exon:16312;Parent=Msa0258180-mRNA-1
chr2_2 exon 54378057 54378900 54378057 ID=Msa0258180-mRNA-1:exon:16311;Parent=Msa0258180-mRNA-1
chr2_2 five_prime_UTR 54378669 54378900 54378669 ID=Msa0258180-mRNA-1:five_prime_utr;Parent=Msa0258180-mRNA-1
chr2_2 CDS 54378057 54378668 54378057 ID=Msa0258180-mRNA-1:cds;Parent=Msa0258180-mRNA-1
chr2_2 CDS 54377595 54377845 54377595 ID=Msa0258180-mRNA-1:cds;Parent=Msa0258180-mRNA-1
chr2_2 CDS 54376393 54376861 54376393 ID=Msa0258180-mRNA-1:cds;Parent=Msa0258180-mRNA-1
chr2_2 three_prime_UTR 54375937 54376392 54375937 ID=Msa0258180-mRNA-1:three_prime_utr;Parent=Msa0258180-mRNA-1
Gene Sequence

>Msa0258180

ATGTTGGAACTAAAGACTCCCCTCAAGAACAAAGGATTCTATGTTAGAATGAAACTCATGAACAAACATAACACAAAACCTCAACAAGACCATCATAAAACATGTTTCTTTTTTACATATTACAAATGGCTTCTTTGGTTTTCTCTTTCTCTTTATTTCTTCACCTCTTATCTCATCACCAATGACCCACAAGACACAACAAACATTGTCTCAAATTCTAACTCCAACGTTGTTTCTCATAACTCATTAGATATGTTGAAAAATTTGAAGGTGTTTGTTTATGAGCTACCTTCAAAATACAACAATGATTGGCTTGTAAATGGGAGGTGTAAAACACATTTGTTTGCTTCTGAAGTTGCTATTCACACCGCATTGTTGAAAAGTGATGTGAGAACTTTTGACCCTTATGAAGCTGATTTCTTCTTTGTTCCTGTTTATGTTTCTTGCAACTTCAGCACGGTTAACGGTTTTCCGGCTATTGGACATGCCCGTGCTCTCATTTCCTCCGCCGTTAATCTCGTCTCCACCGAATATCCATTTTGGAATCGAAGCAGAGGATCCGACCATGTTTTCGTCGCTTCTCATGATTTTGGCGCATGTTTCCATACCCTGGAGGATAAGGCAATGGACGATGGAATACCACAAATATTGAAGAATTCAATTGTGTTGCAGACATTTGGGGTTGAATATGAACATCCTTGTCAAGATGTTGAGAATGTGGTTATACCGCCTTATGTCTCGCCGGAGAAAGTTTCGAGGACGCTTGAGAAAGCTCCGGTGAACGGACGGCGAGATATTTGGGCTTTCTTTAGAGGGAAAATGGAAGTTCATCCTAAAAATGTTAGTGGACGGTATTACAGCAAGCGAGTGCGAACCGAGATATGGAGGAAATTCAACAGCGATCGAAGATTTTATCTACAACGACATAGATTTTCAGGTTACCAAATGGAAATTGCACGATCGGTATTCTGTCTATGTCCACTAGGGTGGGCACCATGGAGTCCGAGGCTTGTAGAATCAGTTGCACTCGGCTGTGTGCCCGTTATAATTGCCGATGGAATACGTTTGCCATTTTCCTCTACTGTGAAATGGCCGGAAATATCACTCACGGTGGCAGAGAAAGACGTAGGAAAACTCGACAAAATTCTCAAGCGCGTGGCAGCGACGAACCTAACTCAAATTCAGAAGAATCTGTGGGACCCAAAAACAAGACAGGCCCTACTTTTCAACAGTGAGGTCCAAGAAGGGGATGCCACGTGGCAAGTATTATTTAGTCTAAGTCAGAAGCTTGGTAGGTCCTACCGTAGGTCCATGGTTTCTAGCCAATTGTGA
Protein sequence

>Msa0258180

MLELKTPLKNKGFYVRMKLMNKHNTKPQQDHHKTCFFFTYYKWLLWFSLSLYFFTSYLITNDPQDTTNIVSNSNSNVVSHNSLDMLKNLKVFVYELPSKYNNDWLVNGRCKTHLFASEVAIHTALLKSDVRTFDPYEADFFFVPVYVSCNFSTVNGFPAIGHARALISSAVNLVSTEYPFWNRSRGSDHVFVASHDFGACFHTLEDKAMDDGIPQILKNSIVLQTFGVEYEHPCQDVENVVIPPYVSPEKVSRTLEKAPVNGRRDIWAFFRGKMEVHPKNVSGRYYSKRVRTEIWRKFNSDRRFYLQRHRFSGYQMEIARSVFCLCPLGWAPWSPRLVESVALGCVPVIIADGIRLPFSSTVKWPEISLTVAEKDVGKLDKILKRVAATNLTQIQKNLWDPKTRQALLFNSEVQEGDATWQVLFSLSQKLGRSYRRSMVSSQL*