AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0218510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218510 MtrunA17_Chr2g0320691 97.161 634 18 0 1 634 1 634 0.0 1281
Msa0218510 MtrunA17_Chr2g0303541 46.154 611 302 10 6 603 9 605 0.0 565
Msa0218510 MtrunA17_Chr8g0374011 39.130 667 340 17 13 629 10 660 3.60e-152 454
Msa0218510 MtrunA17_Chr4g0050021 36.738 607 336 10 6 576 27 621 4.31e-137 416
Msa0218510 MtrunA17_Chr2g0285511 37.329 584 326 7 6 577 9 564 8.94e-137 412
Msa0218510 MtrunA17_Chr1g0199631 38.370 589 325 10 41 619 8 568 2.03e-136 410
Msa0218510 MtrunA17_Chr2g0279481 35.691 622 343 11 6 583 27 635 6.29e-125 384
Msa0218510 MtrunA17_Chr1g0181181 35.915 568 316 8 10 570 13 539 8.32e-124 378
Msa0218510 MtrunA17_Chr4g0010091 35.921 554 326 7 23 571 34 563 1.34e-119 369
Msa0218510 MtrunA17_Chr5g0401451 35.726 585 337 13 7 584 24 576 1.12e-114 356
Msa0218510 MtrunA17_Chr7g0245201 33.548 620 357 14 18 631 58 628 5.93e-108 339
Msa0218510 MtrunA17_Chr7g0268781 34.354 588 338 12 21 591 34 590 7.74e-104 328
Msa0218510 MtrunA17_Chr8g0382231 30.508 590 335 10 15 582 44 580 5.04e-102 323
Msa0218510 MtrunA17_Chr4g0053841 33.278 607 363 15 16 609 6 583 5.36e-101 320
Msa0218510 MtrunA17_Chr1g0195911 33.950 595 330 13 21 592 34 588 7.94e-101 320
Msa0218510 MtrunA17_Chr5g0401341 38.716 483 259 9 23 495 27 482 2.34e-100 319
Msa0218510 MtrunA17_Chr1g0207461 33.746 566 319 10 10 546 13 551 2.27e-95 306
Msa0218510 MtrunA17_Chr1g0150191 34.927 481 289 6 21 495 25 487 2.06e-94 303
Msa0218510 MtrunA17_Chr3g0108331 32.979 564 320 11 2 563 3 510 1.83e-92 296
Msa0218510 MtrunA17_Chr5g0432861 33.278 607 330 12 4 597 6 550 4.30e-88 285
Msa0218510 MtrunA17_Chr8g0367891 33.024 539 295 10 26 536 29 529 6.54e-87 283
Msa0218510 MtrunA17_Chr5g0412591 34.800 500 264 10 21 494 24 487 4.03e-85 278
Msa0218510 MtrunA17_Chr4g0076521 34.454 476 284 9 21 494 24 473 8.46e-85 277
Msa0218510 MtrunA17_Chr1g0191711 27.739 566 342 8 13 576 48 548 2.10e-79 261
Msa0218510 MtrunA17_Chr2g0290741 31.579 475 289 9 21 494 24 463 1.72e-70 238
Msa0218510 MtrunA17_Chr4g0023801 28.055 581 348 18 20 580 2 532 5.55e-56 199
Msa0218510 MtrunA17_Chr4g0003891 36.177 293 162 4 209 497 43 314 1.30e-53 190
Msa0218510 MtrunA17_Chr4g0037661 27.677 495 308 10 26 500 6 470 6.52e-51 184
Msa0218510 MtrunA17_Chr2g0332281 34.843 287 170 3 212 497 24 294 3.26e-48 174
Msa0218510 MtrunA17_Chr1g0163311 25.132 569 335 18 36 576 17 522 1.32e-47 175
Msa0218510 MtrunA17_Chr2g0280541 26.055 403 242 10 91 482 1 358 8.27e-32 128
Msa0218510 MtrunA17_Chr1g0206621 34.058 138 91 0 31 168 76 213 7.06e-23 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218510 AT3G44820.1 63.761 654 211 11 1 634 1 648 0.0 822
Msa0218510 AT1G30440.1 44.949 594 305 7 6 583 9 596 2.55e-180 526
Msa0218510 AT5G03250.1 40.492 610 300 12 6 594 9 576 2.29e-148 442
Msa0218510 AT5G13600.1 40.204 587 297 15 10 582 13 559 1.59e-140 422
Msa0218510 AT5G66560.1 37.988 666 320 15 13 603 9 656 9.39e-138 417
Msa0218510 AT3G50840.1 38.358 597 307 13 17 603 10 555 2.99e-130 395
Msa0218510 AT5G64330.1 34.203 690 346 14 6 600 35 711 5.12e-128 395
Msa0218510 AT5G66560.3 37.209 645 312 14 34 603 1 627 8.92e-128 391
Msa0218510 AT5G66560.4 40.000 570 291 13 67 603 66 617 1.98e-126 387
Msa0218510 AT5G64330.2 36.170 611 287 12 6 521 35 637 3.51e-123 380
Msa0218510 AT3G50840.2 37.982 545 282 11 67 603 23 519 1.50e-115 356
Msa0218510 AT3G50840.3 37.982 545 282 11 67 603 23 519 1.50e-115 356
Msa0218510 AT5G48800.1 35.450 567 318 9 23 581 41 567 1.06e-114 356
Msa0218510 AT5G03250.2 38.552 511 241 12 110 594 1 464 1.78e-110 341
Msa0218510 AT5G64330.3 35.112 581 274 12 36 521 6 578 2.44e-108 339
Msa0218510 AT3G08570.1 34.397 564 326 9 18 571 31 560 2.44e-106 335
Msa0218510 AT3G08660.1 34.403 561 302 11 18 571 31 532 8.17e-104 327
Msa0218510 AT5G67385.1 33.515 549 331 8 7 553 9 525 9.88e-103 325
Msa0218510 AT2G14820.2 34.568 567 314 13 1 544 4 536 3.02e-99 317
Msa0218510 AT2G14820.1 34.568 567 314 13 1 544 4 536 3.02e-99 317
Msa0218510 AT1G03010.2 32.808 634 371 13 21 632 7 607 3.21e-99 316
Msa0218510 AT1G03010.3 32.716 648 379 14 7 632 21 633 3.30e-99 317
Msa0218510 AT1G03010.1 32.808 634 371 13 21 632 34 634 1.03e-98 315
Msa0218510 AT2G30520.1 33.759 548 316 8 16 562 23 524 5.75e-98 312
Msa0218510 AT3G08660.3 33.984 512 277 9 67 571 15 472 8.85e-94 299
Msa0218510 AT5G66560.2 36.939 490 235 11 13 446 9 480 2.82e-92 293
Msa0218510 AT5G10250.2 30.169 590 339 11 8 573 35 575 3.62e-92 298
Msa0218510 AT5G67385.4 33.333 513 306 9 44 553 1 480 1.41e-91 294
Msa0218510 AT5G67385.5 33.333 513 306 9 44 553 1 480 1.41e-91 294
Msa0218510 AT5G67385.2 33.333 513 306 9 44 553 1 480 1.41e-91 294
Msa0218510 AT5G67385.3 33.333 513 306 9 44 553 1 480 1.41e-91 294
Msa0218510 AT5G67440.3 35.611 483 275 8 23 499 27 479 1.75e-91 295
Msa0218510 AT5G67440.1 35.611 483 275 8 23 499 27 479 1.75e-91 295
Msa0218510 AT1G67900.5 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT1G67900.1 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT1G67900.3 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT1G67900.6 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT1G67900.2 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT1G67900.4 37.000 500 273 7 21 494 24 507 1.75e-90 293
Msa0218510 AT2G47860.1 31.579 608 353 13 21 603 35 604 2.10e-90 293
Msa0218510 AT2G30520.2 33.589 521 299 8 43 562 2 476 2.12e-89 288
Msa0218510 AT2G30520.3 33.589 521 299 8 43 562 2 476 2.12e-89 288
Msa0218510 AT5G10250.1 30.225 579 338 11 8 573 35 560 2.48e-89 290
Msa0218510 AT5G47800.3 34.801 477 276 9 24 494 27 474 1.31e-86 281
Msa0218510 AT5G47800.1 34.801 477 276 9 24 494 27 474 1.31e-86 281
Msa0218510 AT5G47800.4 34.801 477 276 9 24 494 27 474 1.31e-86 281
Msa0218510 AT2G47860.3 30.428 631 353 14 21 603 35 627 3.67e-85 280
Msa0218510 AT5G47800.2 34.801 477 276 9 24 494 140 587 5.40e-85 280
Msa0218510 AT5G67440.2 35.043 468 268 8 38 499 5 442 5.85e-85 276
Msa0218510 AT4G31820.1 33.951 486 273 11 18 495 22 467 2.45e-83 273
Msa0218510 AT2G23050.1 34.310 478 250 9 33 503 37 457 2.61e-82 268
Msa0218510 AT4G37590.1 34.196 541 294 14 1 529 4 494 3.21e-82 270
Msa0218510 AT3G26490.1 31.707 533 303 10 21 520 24 528 6.19e-81 267
Msa0218510 AT3G26490.3 31.707 533 303 10 21 520 24 528 6.19e-81 267
Msa0218510 AT3G26490.4 31.707 533 303 10 21 520 24 528 6.19e-81 267
Msa0218510 AT3G49970.1 30.280 535 287 12 31 563 2 452 5.14e-74 246
Msa0218510 AT3G49970.2 30.280 535 287 12 31 563 2 452 5.14e-74 246
Msa0218510 AT4G31820.2 33.484 442 248 10 62 495 11 414 1.66e-72 243
Msa0218510 AT3G08660.2 32.339 436 240 8 18 447 31 417 6.07e-70 233
Msa0218510 AT2G47860.5 29.480 519 308 11 110 603 1 486 5.63e-64 220
Msa0218510 AT2G47860.2 29.480 519 308 11 110 603 1 486 5.63e-64 220
Msa0218510 AT3G26490.2 30.283 459 262 8 21 446 24 457 2.90e-63 218
Msa0218510 AT2G47860.4 31.544 447 248 10 21 446 35 444 3.67e-61 210
Msa0218510 AT2G23050.2 31.156 398 213 8 110 503 1 341 5.15e-57 197
Msa0218510 AT2G47860.6 30.000 470 248 11 21 446 35 467 2.95e-56 198
Msa0218510 AT3G19850.1 28.319 452 274 6 65 499 50 468 1.29e-54 195
Msa0218510 AT5G48130.1 24.788 589 375 16 17 590 33 568 1.68e-50 185
Msa0218510 AT1G52770.1 35.395 291 169 4 212 497 28 304 5.48e-50 180
Msa0218510 AT5G17580.2 27.551 490 295 10 25 502 22 463 2.28e-48 178
Msa0218510 AT5G17580.1 27.551 490 295 10 25 502 7 448 2.58e-48 178
Msa0218510 AT1G50280.2 27.602 442 274 11 65 490 16 427 4.61e-48 176
Msa0218510 AT1G50280.1 27.602 442 274 11 65 490 49 460 8.05e-48 176
Msa0218510 AT3G22104.1 26.950 564 315 13 25 574 6 486 2.29e-47 174
Msa0218510 AT3G03510.1 29.062 437 228 11 75 502 4 367 2.31e-47 173
Msa0218510 AT3G15570.1 33.445 299 168 4 212 497 29 309 1.71e-46 171
Msa0218510 AT3G22104.4 26.122 490 268 10 97 574 4 411 1.84e-37 145
Msa0218510 AT3G22104.2 26.122 490 268 10 97 574 4 411 1.84e-37 145
Msa0218510 AT3G22104.3 26.122 490 268 10 97 574 4 411 1.84e-37 145
Msa0218510 AT3G49900.1 30.556 180 108 5 396 569 214 382 1.09e-13 74.3
Msa0218510 AT3G49900.2 29.508 183 109 5 396 569 214 385 3.46e-12 69.7

Find 166 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 2_1:+65679583 Msa0218510:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 2_1:+65679832 Msa0218510:intron
ATGGCACCTGCAAGGCTTTC+GGG 0.203178 2_1:-65681270 None:intergenic
GCAACAATCAAGAAAATTTC+AGG 0.212225 2_1:-65680784 None:intergenic
TTTCTGTGTGTCTGGACTTA+TGG 0.216589 2_1:-65682518 None:intergenic
TGCACGGACCCGAGTTTATT+TGG 0.228486 2_1:+65681066 Msa0218510:CDS
TTAGTATTTAACTGAGAATT+TGG 0.230331 2_1:+65679514 Msa0218510:five_prime_UTR
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 2_1:+65679584 Msa0218510:CDS
TGCTGTTGTGCGCGAAAATC+AGG 0.238977 2_1:+65682257 Msa0218510:CDS
TCGAAACCCGTAACAGAATT+TGG 0.239506 2_1:-65680806 None:intergenic
GTTCTTCTCATAGGCATTCT+TGG 0.242500 2_1:-65680728 None:intergenic
CAACTGGAAAGATTGTATTT+TGG 0.263796 2_1:+65680957 Msa0218510:CDS
CGTTGATCAGAGGGTTCTTT+TGG 0.270046 2_1:+65681401 Msa0218510:CDS
TCAGGGTTTGAAGGTGGATA+TGG 0.270583 2_1:+65682275 Msa0218510:CDS
GCCCGGGTGGAAGCATTCTT+TGG 0.292191 2_1:+65681121 Msa0218510:CDS
TCAAGCTTGCCAGGAATCTT+TGG 0.296925 2_1:+65681518 Msa0218510:CDS
TATGGCACCTGCAAGGCTTT+CGG 0.307178 2_1:-65681271 None:intergenic
ATTTGGTTAACAAAGTAAAA+AGG 0.310161 2_1:+65679531 Msa0218510:five_prime_UTR
AATATTGGAGATGCAAAGTT+TGG 0.317613 2_1:+65682919 Msa0218510:three_prime_UTR
ATGAAAATAAAGAGGAAATA+TGG 0.321948 2_1:-65682563 None:intergenic
TTCTGTGTGTCTGGACTTAT+GGG 0.322141 2_1:-65682517 None:intergenic
TTATTTCCTTTGCTTCAAAA+TGG 0.324437 2_1:+65679561 Msa0218510:exon
GCCAGGTCTTGGTCGGTGGC+AGG 0.331550 2_1:+65681317 Msa0218510:CDS
CGTATTCGTCCGTCATTTCA+AGG 0.342271 2_1:-65680879 None:intergenic
CTTTCTGGGTTTCATCTTCA+AGG 0.343380 2_1:+65679598 Msa0218510:CDS
AGCTGAAAATCTTCATTTAG+TGG 0.344621 2_1:+65681011 Msa0218510:CDS
GTGCCATAATGTATTATTCT+AGG 0.366832 2_1:+65681286 Msa0218510:CDS
TTCTTCTCTCCAAAGATTCC+TGG 0.369534 2_1:-65681527 None:intergenic
ATAGAATAGGCTCAGAGCTT+TGG 0.373664 2_1:-65680984 None:intergenic
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 2_1:+65679833 Msa0218510:intron
TTGTTGCCAAATTCTGTTAC+GGG 0.377436 2_1:+65680800 Msa0218510:CDS
AGGAAGCACTTGCCAGGTCT+TGG 0.387407 2_1:+65681306 Msa0218510:CDS
ATGAGACCCGAAAGCCTTGC+AGG 0.391213 2_1:+65681264 Msa0218510:CDS
GTCGATCGAGCAGGAACTGC+TGG 0.391220 2_1:-65682186 None:intergenic
TAAATACCGGCGCTAGGATT+CGG 0.393615 2_1:+65681151 Msa0218510:CDS
CTTCCTAGAATAATACATTA+TGG 0.393701 2_1:-65681289 None:intergenic
ACCGCGTGCACTGTGAGATT+TGG 0.397256 2_1:-65682370 None:intergenic
GGCATTCTTGGATTCTTCAT+AGG 0.400386 2_1:-65680716 None:intergenic
GAATCTTTGGAGAGAAGAAT+AGG 0.402865 2_1:+65681531 Msa0218510:CDS
GTGGTACTGGAAGAATTTCC+TGG 0.407573 2_1:+65680757 Msa0218510:CDS
ACAGAACGATCAAGGGGTTC+AGG 0.408900 2_1:-65682456 None:intergenic
GTGATCAAAAGAAAGAATAT+TGG 0.417030 2_1:+65682904 Msa0218510:three_prime_UTR
GAAACTTCTTCCTCAAAAGA+AGG 0.424762 2_1:+65681434 Msa0218510:CDS
AGCACTAGACATATATTTCA+AGG 0.428043 2_1:+65681872 Msa0218510:CDS
TTTCCTCTTGTTTCAAAATG+TGG 0.437919 2_1:+65680679 Msa0218510:CDS
GGTTGAGCATCTGAAAATCT+AGG 0.440908 2_1:-65682435 None:intergenic
CCAGGTCTTGGTCGGTGGCA+GGG 0.442080 2_1:+65681318 Msa0218510:CDS
TTTAAACGCTCTATCTATGA+TGG 0.443227 2_1:+65681041 Msa0218510:CDS
TTCTTTCCCTCATGTAAAGT+TGG 0.443597 2_1:+65682719 Msa0218510:three_prime_UTR
TGCTTCAAAATGGCTCCACC+TGG 0.443870 2_1:+65679571 Msa0218510:exon
ATTGTTGCCAAATTCTGTTA+CGG 0.444459 2_1:+65680799 Msa0218510:CDS
ATTCCATAAGTACAAAATGA+TGG 0.452056 2_1:-65681650 None:intergenic
CGGTTACTTGCAAGAAAACT+CGG 0.454375 2_1:+65682399 Msa0218510:CDS
GATAGAGTAACTGAGATATT+CGG 0.456605 2_1:+65679456 Msa0218510:five_prime_UTR
CTTTGGAATGGTATAAATAC+CGG 0.458255 2_1:+65681138 Msa0218510:CDS
AATCTTTGGAGAGAAGAATA+GGG 0.459226 2_1:+65681532 Msa0218510:CDS
GATATTACTGTGTCAATTGA+TGG 0.459358 2_1:+65679865 Msa0218510:CDS
AGCCGGATGTGTCAATGCTT+TGG 0.460297 2_1:+65682149 Msa0218510:CDS
CTCATGCATCTCAAAATGAT+AGG 0.461872 2_1:+65682064 Msa0218510:CDS
TGATCAACGGCAGCACTAGC+AGG 0.464029 2_1:-65681387 None:intergenic
AGGAGAAAAGAAAAGTATGA+AGG 0.466174 2_1:-65679376 None:intergenic
AAGTTGATAGATAGCTATAT+TGG 0.467102 2_1:+65681750 Msa0218510:CDS
CATCGTCGTCTGAATCGTTA+AGG 0.468385 2_1:+65681718 Msa0218510:CDS
GAGAAGAATAGGGATGCAAC+TGG 0.469240 2_1:+65681542 Msa0218510:CDS
GAATGTTGATCAAGCTTGCC+AGG 0.470324 2_1:+65681509 Msa0218510:CDS
GGTCGGTGGCAGGGTGGACA+AGG 0.471322 2_1:+65681327 Msa0218510:CDS
GTCAGAAATTAAGTAAAGTC+TGG 0.471560 2_1:+65679478 Msa0218510:five_prime_UTR
GGGAAGGGAAGCGAGAAGAA+AGG 0.472367 2_1:-65679355 None:intergenic
TATTCTAGGAAGCACTTGCC+AGG 0.478232 2_1:+65681300 Msa0218510:CDS
TTCATCTTCAAGGTCATCAC+TGG 0.484382 2_1:+65679608 Msa0218510:CDS
ACACTTTGCAGGCTCATCCT+TGG 0.485150 2_1:+65681971 Msa0218510:intron
CTTTACATGAGGGAAAGAAA+TGG 0.488916 2_1:-65682715 None:intergenic
ATGAATAAATGAAAATAAAG+AGG 0.488926 2_1:-65682571 None:intergenic
GGAGAAAAGAAAAGTATGAA+GGG 0.491413 2_1:-65679375 None:intergenic
GTGTGGATACAGAACGATCA+AGG 0.491533 2_1:-65682464 None:intergenic
GAAGAACCTGACAGTGGTAC+TGG 0.491597 2_1:+65680744 Msa0218510:CDS
CACTGTCAGGTTCTTCTCAT+AGG 0.491878 2_1:-65680737 None:intergenic
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 2_1:-65679589 None:intergenic
AAAAGAAAAGTATGAAGGGA+AGG 0.494164 2_1:-65679371 None:intergenic
CGGACCCGAGTTTATTTGGA+TGG 0.499303 2_1:+65681070 Msa0218510:CDS
GGATGGCCGATGATGATGTA+TGG 0.500030 2_1:+65681087 Msa0218510:CDS
GCCCGCTCGACTGATGCTCT+TGG 0.504243 2_1:-65682486 None:intergenic
CCTATGTGTTGATAAATACA+AGG 0.512785 2_1:-65682810 None:intergenic
GCATCAGAATCTGCATAAGC+AGG 0.514844 2_1:-65681594 None:intergenic
ATCCAAAGCATTGACACATC+CGG 0.515020 2_1:-65682151 None:intergenic
AAGTGCATACCTTGTGAAGA+TGG 0.517267 2_1:-65679897 None:intergenic
GGAGAAATTGTACAAAGAAA+CGG 0.517355 2_1:+65682228 Msa0218510:CDS
ATTCTTCCAGTACCACTGTC+AGG 0.520265 2_1:-65680750 None:intergenic
ATCAAGCGATGAAGGAGAAA+AGG 0.522933 2_1:-65681685 None:intergenic
CATTTGAGAACAGCATTAGC+CGG 0.524224 2_1:+65682132 Msa0218510:CDS
CCCTGCCACCGACCAAGACC+TGG 0.524414 2_1:-65681318 None:intergenic
CTAGTGCTGCCGTTGATCAG+AGG 0.525231 2_1:+65681391 Msa0218510:CDS
GCTTGCGGATCAAGCGATGA+AGG 0.528515 2_1:-65681693 None:intergenic
ACTGCTCGAGGTGGTACGGC+TGG 0.530026 2_1:+65682207 Msa0218510:CDS
ACCAAGAGCATCAGTCGAGC+GGG 0.538088 2_1:+65682485 Msa0218510:CDS
CCTTGTATTTATCAACACAT+AGG 0.544116 2_1:+65682810 Msa0218510:three_prime_UTR
ACAGTGCACGCGGTTCTCCT+CGG 0.546364 2_1:+65682379 Msa0218510:CDS
GCGCGAAAATCAGGGTTTGA+AGG 0.547646 2_1:+65682266 Msa0218510:CDS
TGGTACTGGAAGAATTTCCT+GGG 0.549040 2_1:+65680758 Msa0218510:CDS
TTCCAAAGAATGCTTCCACC+CGG 0.549351 2_1:-65681123 None:intergenic
GACTTGAAGAACAACTCTGA+GGG 0.551619 2_1:-65682091 None:intergenic
TAGTGCTGCCGTTGATCAGA+GGG 0.552150 2_1:+65681392 Msa0218510:CDS
TCTATCTATGATGGCTTGCA+CGG 0.554280 2_1:+65681050 Msa0218510:CDS
AAACTTCTTCCTCAAAAGAA+GGG 0.557294 2_1:+65681435 Msa0218510:CDS
GGTGGAAGCATTCTTTGGAA+TGG 0.559847 2_1:+65681126 Msa0218510:CDS
GCTGTTGTGCGCGAAAATCA+GGG 0.560005 2_1:+65682258 Msa0218510:CDS
AAAGAAAAGTATGAAGGGAA+GGG 0.563336 2_1:-65679370 None:intergenic
TCCAAAGAATGCTTCCACCC+GGG 0.565201 2_1:-65681122 None:intergenic
ATCGGCCATCCAAATAAACT+CGG 0.566431 2_1:-65681075 None:intergenic
ACTTGCCAGGTCTTGGTCGG+TGG 0.567054 2_1:+65681313 Msa0218510:CDS
AAACCACCACTCGGATTCTG+AGG 0.569410 2_1:-65681175 None:intergenic
ATGATCTCGATGACTCAGGA+AGG 0.570271 2_1:-65681830 None:intergenic
TGCAATGATCTCGATGACTC+AGG 0.571453 2_1:-65681834 None:intergenic
CTCGACTGATGCTCTTGGTG+TGG 0.573680 2_1:-65682481 None:intergenic
ATGGTATAAATACCGGCGCT+AGG 0.573781 2_1:+65681145 Msa0218510:CDS
CGAAAATCAGGGTTTGAAGG+TGG 0.575204 2_1:+65682269 Msa0218510:CDS
CCTCGAGCAGTCGATCGAGC+AGG 0.575521 2_1:-65682195 None:intergenic
ATCGACTGCTCGAGGTGGTA+CGG 0.579037 2_1:+65682203 Msa0218510:CDS
ATGACGGACGAATACGAAGA+AGG 0.579734 2_1:+65680886 Msa0218510:CDS
GAAGAAGTCCGAGTAAGAAA+CGG 0.583295 2_1:-65681470 None:intergenic
CGGTGGCAGGGTGGACAAGG+TGG 0.592330 2_1:+65681330 Msa0218510:CDS
TGTGGATACAGAACGATCAA+GGG 0.592917 2_1:-65682463 None:intergenic
TTCTCGTTATCAGAGAGCCA+AGG 0.599221 2_1:-65681988 None:intergenic
CAAAAGAACCCTCTGATCAA+CGG 0.599347 2_1:-65681400 None:intergenic
AGCACTTGCCAGGTCTTGGT+CGG 0.602120 2_1:+65681310 Msa0218510:CDS
CACCAAGAGCATCAGTCGAG+CGG 0.602703 2_1:+65682484 Msa0218510:CDS
TGAAATATATGTCTAGTGCT+CGG 0.602823 2_1:-65681869 None:intergenic
GCTTCAAAATGGCTCCACCT+GGG 0.603801 2_1:+65679572 Msa0218510:exon
GAGATGCATGAGCACATGCA+TGG 0.604027 2_1:-65682053 None:intergenic
TCTCAGTTACTCTATCATCA+TGG 0.604133 2_1:-65679449 None:intergenic
AATTGTACAAAGAAACGGAT+GGG 0.604670 2_1:+65682233 Msa0218510:CDS
TGCAGAATACCTTGAAATGA+CGG 0.605457 2_1:+65680870 Msa0218510:CDS
CCTGCTCGATCGACTGCTCG+AGG 0.610208 2_1:+65682195 Msa0218510:CDS
TGCACTGTGAGATTTGGTCA+CGG 0.613993 2_1:-65682364 None:intergenic
ATTAAAACGATGCGAGCGAG+AGG 0.617106 2_1:+65681240 Msa0218510:CDS
AAACTCCCATACATCATCAT+CGG 0.620072 2_1:-65681093 None:intergenic
GATAGAATGAGTACTAGAGT+CGG 0.620497 2_1:+65682297 Msa0218510:CDS
GATGGCCGATGATGATGTAT+GGG 0.621720 2_1:+65681088 Msa0218510:CDS
GGTCTTGGTCGGTGGCAGGG+TGG 0.626378 2_1:+65681321 Msa0218510:CDS
GGATGTGTCAATGCTTTGGA+TGG 0.630355 2_1:+65682153 Msa0218510:CDS
GGACCTCAGAATCCGAGTGG+TGG 0.632921 2_1:+65681172 Msa0218510:CDS
TGATCTCGATGACTCAGGAA+GGG 0.637384 2_1:-65681829 None:intergenic
TGAGGTCCGAATCCTAGCGC+CGG 0.638429 2_1:-65681157 None:intergenic
GATGTCTTCAAACCACCACT+CGG 0.642289 2_1:-65681184 None:intergenic
AATACATTATGGCACCTGCA+AGG 0.648443 2_1:-65681278 None:intergenic
GGTTACTTGCAAGAAAACTC+GGG 0.650025 2_1:+65682400 Msa0218510:CDS
TCAGACGACGATGATGCTTG+CGG 0.656201 2_1:-65681708 None:intergenic
ATTGACACAGTAATATCACT+TGG 0.657084 2_1:-65679859 None:intergenic
TCGGCCATCCAAATAAACTC+GGG 0.657524 2_1:-65681074 None:intergenic
AAATTGTACAAAGAAACGGA+TGG 0.658614 2_1:+65682232 Msa0218510:CDS
AGACTTGAAGAACAACTCTG+AGG 0.663274 2_1:-65682092 None:intergenic
CTATGTGTTGATAAATACAA+GGG 0.664074 2_1:-65682809 None:intergenic
TGTCAATTTCCATCTTCACA+AGG 0.664191 2_1:+65679888 Msa0218510:CDS
ACCAAATCTCACAGTGCACG+CGG 0.665622 2_1:+65682369 Msa0218510:CDS
TGTGTCTGGACTTATGGGAA+CGG 0.677659 2_1:-65682512 None:intergenic
TCATCGAGATCATTGCATGA+CGG 0.684043 2_1:+65681840 Msa0218510:CDS
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 2_1:-65679586 None:intergenic
GTGGATACAGAACGATCAAG+GGG 0.686942 2_1:-65682462 None:intergenic
GCTCGATCGACTGCTCGAGG+TGG 0.696171 2_1:+65682198 Msa0218510:CDS
GGTACTGGAAGAATTTCCTG+GGG 0.699090 2_1:+65680759 Msa0218510:CDS
ACTTGAAGAACAACTCTGAG+GGG 0.701967 2_1:-65682090 None:intergenic
TTCGGACCTCAGAATCCGAG+TGG 0.719040 2_1:+65681169 Msa0218510:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 2_1:-65680861 None:intergenic
ATACACATGAAAGTGTTGTG+TGG 0.736317 2_1:+65682596 Msa0218510:three_prime_UTR
CTATGAGAAGAACCTGACAG+TGG 0.742704 2_1:+65680738 Msa0218510:CDS
GTACTGGAAGAATTTCCTGG+GGG 0.781656 2_1:+65680760 Msa0218510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAAAGAAAGAAAA+AGG - chr2_1:65679399-65679418 None:intergenic 10.0%
!! CTTCTTTATTATTTATTTAT+TGG + chr2_1:65679655-65679674 Msa0218510:intron 10.0%
!!! TTTTTTTTTTCCTGTTTTTT+TGG + chr2_1:65679801-65679820 Msa0218510:intron 15.0%
!! TATTATGAATAGATTATTAG+TGG - chr2_1:65679951-65679970 None:intergenic 15.0%
!! TATTCATAATAATTTGATAG+TGG + chr2_1:65679960-65679979 Msa0218510:intron 15.0%
!! AATCAAAATAACAGTAAAAT+AGG - chr2_1:65680094-65680113 None:intergenic 15.0%
!!! AAAACTTTTTTACATCAATA+GGG - chr2_1:65680125-65680144 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG - chr2_1:65680126-65680145 None:intergenic 15.0%
!! AAGTAATTTATCATATCAAA+AGG - chr2_1:65680613-65680632 None:intergenic 15.0%
!! AGAATTCAGTAAAATAAAAA+AGG + chr2_1:65682332-65682351 Msa0218510:CDS 15.0%
!! ATGAATAAATGAAAATAAAG+AGG - chr2_1:65682574-65682593 None:intergenic 15.0%
!! TTAGTATTTAACTGAGAATT+TGG + chr2_1:65679514-65679533 Msa0218510:five_prime_UTR 20.0%
!! ATTTGGTTAACAAAGTAAAA+AGG + chr2_1:65679531-65679550 Msa0218510:five_prime_UTR 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG - chr2_1:65679814-65679833 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG + chr2_1:65679814-65679833 Msa0218510:intron 20.0%
!! ACAAAAGAACAAAAATGAAT+AGG - chr2_1:65680028-65680047 None:intergenic 20.0%
!!! AACTAATGCATTTAAAATGT+TGG - chr2_1:65680396-65680415 None:intergenic 20.0%
!! GATATTGGAGAAAATTTATT+TGG + chr2_1:65680479-65680498 Msa0218510:intron 20.0%
!! ATGAAAATAAAGAGGAAATA+TGG - chr2_1:65682566-65682585 None:intergenic 20.0%
!!! GTTTAAATTTTTTTGTACGT+GGG + chr2_1:65682618-65682637 Msa0218510:three_prime_UTR 20.0%
!! GCTTAAATAAAAATGCATAA+TGG - chr2_1:65682673-65682692 None:intergenic 20.0%
!!! TTTCTTTGTACATTTTTCAT+TGG + chr2_1:65682745-65682764 Msa0218510:three_prime_UTR 20.0%
! TTATTTCCTTTGCTTCAAAA+TGG + chr2_1:65679561-65679580 Msa0218510:exon 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG + chr2_1:65679723-65679742 Msa0218510:intron 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG + chr2_1:65679818-65679837 Msa0218510:intron 25.0%
! TAGATTATTAGTGGAAGAAA+AGG - chr2_1:65679942-65679961 None:intergenic 25.0%
!!! TTGGGATTTTTTTTCTTTTG+TGG + chr2_1:65679991-65680010 Msa0218510:intron 25.0%
!!! TTTTTGACTCTTTACATTTG+CGG + chr2_1:65680238-65680257 Msa0218510:intron 25.0%
!!! AGGTGAATATTTTGAGATAT+TGG + chr2_1:65680464-65680483 Msa0218510:intron 25.0%
! ATTGGAGAAAATTTATTTGG+AGG + chr2_1:65680482-65680501 Msa0218510:intron 25.0%
! AATTACTTGTAATGTTGTCT+TGG + chr2_1:65680625-65680644 Msa0218510:intron 25.0%
! CTTCCTAGAATAATACATTA+TGG - chr2_1:65681292-65681311 None:intergenic 25.0%
! ATTCCATAAGTACAAAATGA+TGG - chr2_1:65681653-65681672 None:intergenic 25.0%
!!! AAGTTGATAGATAGCTATAT+TGG + chr2_1:65681750-65681769 Msa0218510:CDS 25.0%
!!! GGTTTAAATTTTTTTGTACG+TGG + chr2_1:65682617-65682636 Msa0218510:three_prime_UTR 25.0%
! AAAAAACCAACTTTACATGA+GGG - chr2_1:65682728-65682747 None:intergenic 25.0%
!! CTATGTGTTGATAAATACAA+GGG - chr2_1:65682812-65682831 None:intergenic 25.0%
!! ATTTTAGACATCAATTGTGA+AGG + chr2_1:65682873-65682892 Msa0218510:three_prime_UTR 25.0%
! GTGATCAAAAGAAAGAATAT+TGG + chr2_1:65682904-65682923 Msa0218510:three_prime_UTR 25.0%
! AAAGAAAAGTATGAAGGGAA+GGG - chr2_1:65679373-65679392 None:intergenic 30.0%
! AAAAGAAAAGTATGAAGGGA+AGG - chr2_1:65679374-65679393 None:intergenic 30.0%
! GGAGAAAAGAAAAGTATGAA+GGG - chr2_1:65679378-65679397 None:intergenic 30.0%
! AGGAGAAAAGAAAAGTATGA+AGG - chr2_1:65679379-65679398 None:intergenic 30.0%
GATAGAGTAACTGAGATATT+CGG + chr2_1:65679456-65679475 Msa0218510:five_prime_UTR 30.0%
GTCAGAAATTAAGTAAAGTC+TGG + chr2_1:65679478-65679497 Msa0218510:five_prime_UTR 30.0%
TGGTTAACAAAGTAAAAAGG+AGG + chr2_1:65679534-65679553 Msa0218510:five_prime_UTR 30.0%
GGTTAACAAAGTAAAAAGGA+GGG + chr2_1:65679535-65679554 Msa0218510:five_prime_UTR 30.0%
ATTGACACAGTAATATCACT+TGG - chr2_1:65679862-65679881 None:intergenic 30.0%
GATATTACTGTGTCAATTGA+TGG + chr2_1:65679865-65679884 Msa0218510:CDS 30.0%
AGTAAAATAGGTCACAAAGT+AGG - chr2_1:65680082-65680101 None:intergenic 30.0%
TAATAAGTCACACGTATGAT+TGG - chr2_1:65680168-65680187 None:intergenic 30.0%
TTGTGATCTCTAGTTAATGA+GGG - chr2_1:65680307-65680326 None:intergenic 30.0%
ATTGTGATCTCTAGTTAATG+AGG - chr2_1:65680308-65680327 None:intergenic 30.0%
GTTATGTGGCTACAATTAAA+AGG + chr2_1:65680438-65680457 Msa0218510:intron 30.0%
TGGCTACAATTAAAAGGAAA+AGG + chr2_1:65680444-65680463 Msa0218510:intron 30.0%
ATATTTGCACGGTCAAATAA+GGG + chr2_1:65680540-65680559 Msa0218510:intron 30.0%
! TTTCCTCTTGTTTCAAAATG+TGG + chr2_1:65680679-65680698 Msa0218510:CDS 30.0%
!!! TTTCCACATTTTGAAACAAG+AGG - chr2_1:65680685-65680704 None:intergenic 30.0%
TCAAAATGTGGAAAAATTGC+TGG + chr2_1:65680691-65680710 Msa0218510:CDS 30.0%
GCAACAATCAAGAAAATTTC+AGG - chr2_1:65680787-65680806 None:intergenic 30.0%
ATTGTTGCCAAATTCTGTTA+CGG + chr2_1:65680799-65680818 Msa0218510:CDS 30.0%
!! AGTTGCGCATTATGTTTTTA+TGG - chr2_1:65680942-65680961 None:intergenic 30.0%
ATAAAAACATAATGCGCAAC+TGG + chr2_1:65680941-65680960 Msa0218510:CDS 30.0%
! CAACTGGAAAGATTGTATTT+TGG + chr2_1:65680957-65680976 Msa0218510:CDS 30.0%
! TTTTCAGCTCTTGATAGAAT+AGG - chr2_1:65681000-65681019 None:intergenic 30.0%
AGCTGAAAATCTTCATTTAG+TGG + chr2_1:65681011-65681030 Msa0218510:CDS 30.0%
TTTAAACGCTCTATCTATGA+TGG + chr2_1:65681041-65681060 Msa0218510:CDS 30.0%
!! CTTTGGAATGGTATAAATAC+CGG + chr2_1:65681138-65681157 Msa0218510:CDS 30.0%
GTGCCATAATGTATTATTCT+AGG + chr2_1:65681286-65681305 Msa0218510:CDS 30.0%
AAACTTCTTCCTCAAAAGAA+GGG + chr2_1:65681435-65681454 Msa0218510:CDS 30.0%
! AAAGATTTTCCCTTCTTTTG+AGG - chr2_1:65681447-65681466 None:intergenic 30.0%
! AATCTTTGGAGAGAAGAATA+GGG + chr2_1:65681532-65681551 Msa0218510:CDS 30.0%
! TGTCCATCATTTTGTACTTA+TGG + chr2_1:65681647-65681666 Msa0218510:CDS 30.0%
TGAAATATATGTCTAGTGCT+CGG - chr2_1:65681872-65681891 None:intergenic 30.0%
AGCACTAGACATATATTTCA+AGG + chr2_1:65681872-65681891 Msa0218510:CDS 30.0%
GGAGAAATTGTACAAAGAAA+CGG + chr2_1:65682228-65682247 Msa0218510:CDS 30.0%
AAATTGTACAAAGAAACGGA+TGG + chr2_1:65682232-65682251 Msa0218510:CDS 30.0%
AATTGTACAAAGAAACGGAT+GGG + chr2_1:65682233-65682252 Msa0218510:CDS 30.0%
!!! AATTTTTTTGTACGTGGGAA+AGG + chr2_1:65682623-65682642 Msa0218510:three_prime_UTR 30.0%
!!! ATTTTTTTGTACGTGGGAAA+GGG + chr2_1:65682624-65682643 Msa0218510:three_prime_UTR 30.0%
GAAAAAACCAACTTTACATG+AGG - chr2_1:65682729-65682748 None:intergenic 30.0%
!!! ATTGTCTTGTTTTGTTTGTG+TGG + chr2_1:65682779-65682798 Msa0218510:three_prime_UTR 30.0%
!!! TTGTCTTGTTTTGTTTGTGT+GGG + chr2_1:65682780-65682799 Msa0218510:three_prime_UTR 30.0%
! CCTATGTGTTGATAAATACA+AGG - chr2_1:65682813-65682832 None:intergenic 30.0%
CCTTGTATTTATCAACACAT+AGG + chr2_1:65682810-65682829 Msa0218510:three_prime_UTR 30.0%
!! CATTCTTCATTTTGACATAC+TGG + chr2_1:65682835-65682854 Msa0218510:three_prime_UTR 30.0%
TCTCAGTTACTCTATCATCA+TGG - chr2_1:65679452-65679471 None:intergenic 35.0%
AGTTGCATGTATGTATGACT+TGG - chr2_1:65679752-65679771 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG + chr2_1:65679832-65679851 Msa0218510:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG + chr2_1:65679833-65679852 Msa0218510:intron 35.0%
TGTCAATTTCCATCTTCACA+AGG + chr2_1:65679888-65679907 Msa0218510:CDS 35.0%
!! TTTGATAGTGGAATAGCTGT+TGG + chr2_1:65679972-65679991 Msa0218510:intron 35.0%
!! TTGATAGTGGAATAGCTGTT+GGG + chr2_1:65679973-65679992 Msa0218510:intron 35.0%
! CAAAGTAGGAGCACATTAAT+TGG - chr2_1:65680068-65680087 None:intergenic 35.0%
! GTTCACTTGATCTACTGTTT+TGG + chr2_1:65680413-65680432 Msa0218510:intron 35.0%
!!! CTACTGTTTTGGAAGTTATG+TGG + chr2_1:65680424-65680443 Msa0218510:intron 35.0%
GAGAAAATTTATTTGGAGGC+TGG + chr2_1:65680486-65680505 Msa0218510:intron 35.0%
TATTTGACCGTGCAAATATG+CGG - chr2_1:65680539-65680558 None:intergenic 35.0%
CATATTTGCACGGTCAAATA+AGG + chr2_1:65680539-65680558 Msa0218510:intron 35.0%
TATTTGCACGGTCAAATAAG+GGG + chr2_1:65680541-65680560 Msa0218510:intron 35.0%
TTGTTGCCAAATTCTGTTAC+GGG + chr2_1:65680800-65680819 Msa0218510:CDS 35.0%
TGCAGAATACCTTGAAATGA+CGG + chr2_1:65680870-65680889 Msa0218510:CDS 35.0%
AAACTCCCATACATCATCAT+CGG - chr2_1:65681096-65681115 None:intergenic 35.0%
GAAACTTCTTCCTCAAAAGA+AGG + chr2_1:65681434-65681453 Msa0218510:CDS 35.0%
!! TCTTTTTGCCGTTTCTTACT+CGG + chr2_1:65681462-65681481 Msa0218510:CDS 35.0%
! GAATCTTTGGAGAGAAGAAT+AGG + chr2_1:65681531-65681550 Msa0218510:CDS 35.0%
!! ATGCATGGATTGAGAGTTTT+TGG - chr2_1:65682041-65682060 None:intergenic 35.0%
CTCATGCATCTCAAAATGAT+AGG + chr2_1:65682064-65682083 Msa0218510:CDS 35.0%
GATAGAATGAGTACTAGAGT+CGG + chr2_1:65682297-65682316 Msa0218510:CDS 35.0%
AGAAAAACTTTCTGTGTGTC+TGG - chr2_1:65682529-65682548 None:intergenic 35.0%
!! ATACACATGAAAGTGTTGTG+TGG + chr2_1:65682596-65682615 Msa0218510:three_prime_UTR 35.0%
CTTTACATGAGGGAAAGAAA+TGG - chr2_1:65682718-65682737 None:intergenic 35.0%
TTCTTTCCCTCATGTAAAGT+TGG + chr2_1:65682719-65682738 Msa0218510:three_prime_UTR 35.0%
!!! TGTCTTGTTTTGTTTGTGTG+GGG + chr2_1:65682781-65682800 Msa0218510:three_prime_UTR 35.0%
CTTTCTGGGTTTCATCTTCA+AGG + chr2_1:65679598-65679617 Msa0218510:CDS 40.0%
TTCATCTTCAAGGTCATCAC+TGG + chr2_1:65679608-65679627 Msa0218510:CDS 40.0%
AAGTGCATACCTTGTGAAGA+TGG - chr2_1:65679900-65679919 None:intergenic 40.0%
TGAATTCCCGCATATTTGCA+CGG + chr2_1:65680529-65680548 Msa0218510:intron 40.0%
ATTTGACCGTGCAAATATGC+GGG - chr2_1:65680538-65680557 None:intergenic 40.0%
TAAGGGGAATTTACGCGAAA+CGG + chr2_1:65680557-65680576 Msa0218510:intron 40.0%
AAGAGGAAACTGCAGTTTCA+CGG - chr2_1:65680668-65680687 None:intergenic 40.0%
GGCATTCTTGGATTCTTCAT+AGG - chr2_1:65680719-65680738 None:intergenic 40.0%
GTTCTTCTCATAGGCATTCT+TGG - chr2_1:65680731-65680750 None:intergenic 40.0%
! TGGTACTGGAAGAATTTCCT+GGG + chr2_1:65680758-65680777 Msa0218510:CDS 40.0%
TCGAAACCCGTAACAGAATT+TGG - chr2_1:65680809-65680828 None:intergenic 40.0%
ATAGAATAGGCTCAGAGCTT+TGG - chr2_1:65680987-65681006 None:intergenic 40.0%
! TCTATCTATGATGGCTTGCA+CGG + chr2_1:65681050-65681069 Msa0218510:CDS 40.0%
ATCGGCCATCCAAATAAACT+CGG - chr2_1:65681078-65681097 None:intergenic 40.0%
!! TCGCTCGCATCGTTTTAATA+AGG - chr2_1:65681239-65681258 None:intergenic 40.0%
AATACATTATGGCACCTGCA+AGG - chr2_1:65681281-65681300 None:intergenic 40.0%
CAAAAGAACCCTCTGATCAA+CGG - chr2_1:65681403-65681422 None:intergenic 40.0%
GAAGAAGTCCGAGTAAGAAA+CGG - chr2_1:65681473-65681492 None:intergenic 40.0%
TTCTTCTCTCCAAAGATTCC+TGG - chr2_1:65681530-65681549 None:intergenic 40.0%
ATCAAGCGATGAAGGAGAAA+AGG - chr2_1:65681688-65681707 None:intergenic 40.0%
TCATCGAGATCATTGCATGA+CGG + chr2_1:65681840-65681859 Msa0218510:CDS 40.0%
ACTTGAAGAACAACTCTGAG+GGG - chr2_1:65682093-65682112 None:intergenic 40.0%
GACTTGAAGAACAACTCTGA+GGG - chr2_1:65682094-65682113 None:intergenic 40.0%
AGACTTGAAGAACAACTCTG+AGG - chr2_1:65682095-65682114 None:intergenic 40.0%
CATTTGAGAACAGCATTAGC+CGG + chr2_1:65682132-65682151 Msa0218510:CDS 40.0%
!! ATCCAAAGCATTGACACATC+CGG - chr2_1:65682154-65682173 None:intergenic 40.0%
! AGTTTTCTTGCAAGTAACCG+AGG - chr2_1:65682399-65682418 None:intergenic 40.0%
CGGTTACTTGCAAGAAAACT+CGG + chr2_1:65682399-65682418 Msa0218510:CDS 40.0%
GGTTACTTGCAAGAAAACTC+GGG + chr2_1:65682400-65682419 Msa0218510:CDS 40.0%
GGTTGAGCATCTGAAAATCT+AGG - chr2_1:65682438-65682457 None:intergenic 40.0%
TGTGGATACAGAACGATCAA+GGG - chr2_1:65682466-65682485 None:intergenic 40.0%
TTCTGTGTGTCTGGACTTAT+GGG - chr2_1:65682520-65682539 None:intergenic 40.0%
TTTCTGTGTGTCTGGACTTA+TGG - chr2_1:65682521-65682540 None:intergenic 40.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG - chr2_1:65679570-65679589 None:intergenic 45.0%
CACTGTCAGGTTCTTCTCAT+AGG - chr2_1:65680740-65680759 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG + chr2_1:65680738-65680757 Msa0218510:CDS 45.0%
ATTCTTCCAGTACCACTGTC+AGG - chr2_1:65680753-65680772 None:intergenic 45.0%
! GTGGTACTGGAAGAATTTCC+TGG + chr2_1:65680757-65680776 Msa0218510:CDS 45.0%
! GGTACTGGAAGAATTTCCTG+GGG + chr2_1:65680759-65680778 Msa0218510:CDS 45.0%
GTACTGGAAGAATTTCCTGG+GGG + chr2_1:65680760-65680779 Msa0218510:CDS 45.0%
AAGAAAATTTCAGGACCCCC+AGG - chr2_1:65680778-65680797 None:intergenic 45.0%
CGTATTCGTCCGTCATTTCA+AGG - chr2_1:65680882-65680901 None:intergenic 45.0%
!! ATGACGGACGAATACGAAGA+AGG + chr2_1:65680886-65680905 Msa0218510:CDS 45.0%
TCGGCCATCCAAATAAACTC+GGG - chr2_1:65681077-65681096 None:intergenic 45.0%
! GATGGCCGATGATGATGTAT+GGG + chr2_1:65681088-65681107 Msa0218510:CDS 45.0%
TTCCAAAGAATGCTTCCACC+CGG - chr2_1:65681126-65681145 None:intergenic 45.0%
!! GGTGGAAGCATTCTTTGGAA+TGG + chr2_1:65681126-65681145 Msa0218510:CDS 45.0%
ATGGTATAAATACCGGCGCT+AGG + chr2_1:65681145-65681164 Msa0218510:CDS 45.0%
TAAATACCGGCGCTAGGATT+CGG + chr2_1:65681151-65681170 Msa0218510:CDS 45.0%
GATGTCTTCAAACCACCACT+CGG - chr2_1:65681187-65681206 None:intergenic 45.0%
ATTAAAACGATGCGAGCGAG+AGG + chr2_1:65681240-65681259 Msa0218510:CDS 45.0%
!! TATTCTAGGAAGCACTTGCC+AGG + chr2_1:65681300-65681319 Msa0218510:CDS 45.0%
! CGTTGATCAGAGGGTTCTTT+TGG + chr2_1:65681401-65681420 Msa0218510:CDS 45.0%
GAATGTTGATCAAGCTTGCC+AGG + chr2_1:65681509-65681528 Msa0218510:CDS 45.0%
TCAAGCTTGCCAGGAATCTT+TGG + chr2_1:65681518-65681537 Msa0218510:CDS 45.0%
GAGAAGAATAGGGATGCAAC+TGG + chr2_1:65681542-65681561 Msa0218510:CDS 45.0%
GCATCAGAATCTGCATAAGC+AGG - chr2_1:65681597-65681616 None:intergenic 45.0%
CATCGTCGTCTGAATCGTTA+AGG + chr2_1:65681718-65681737 Msa0218510:CDS 45.0%
!! GCAAGAGCACGTAACTTTTC+TGG - chr2_1:65681804-65681823 None:intergenic 45.0%
TGATCTCGATGACTCAGGAA+GGG - chr2_1:65681832-65681851 None:intergenic 45.0%
ATGATCTCGATGACTCAGGA+AGG - chr2_1:65681833-65681852 None:intergenic 45.0%
TGCAATGATCTCGATGACTC+AGG - chr2_1:65681837-65681856 None:intergenic 45.0%
ATCGATCGAGAATGTAGCTC+AGG - chr2_1:65681907-65681926 None:intergenic 45.0%
GAATGCTTACGACACTTTGC+AGG + chr2_1:65681960-65681979 Msa0218510:intron 45.0%
TTCTCGTTATCAGAGAGCCA+AGG - chr2_1:65681991-65682010 None:intergenic 45.0%
! GGATGTGTCAATGCTTTGGA+TGG + chr2_1:65682153-65682172 Msa0218510:CDS 45.0%
CGAAAATCAGGGTTTGAAGG+TGG + chr2_1:65682269-65682288 Msa0218510:CDS 45.0%
TCAGGGTTTGAAGGTGGATA+TGG + chr2_1:65682275-65682294 Msa0218510:CDS 45.0%
! TGCACTGTGAGATTTGGTCA+CGG - chr2_1:65682367-65682386 None:intergenic 45.0%
GTGGATACAGAACGATCAAG+GGG - chr2_1:65682465-65682484 None:intergenic 45.0%
GTGTGGATACAGAACGATCA+AGG - chr2_1:65682467-65682486 None:intergenic 45.0%
TGTGTCTGGACTTATGGGAA+CGG - chr2_1:65682515-65682534 None:intergenic 45.0%
! TTTTGTACGTGGGAAAGGGA+AGG + chr2_1:65682628-65682647 Msa0218510:three_prime_UTR 45.0%
TGCTTCAAAATGGCTCCACC+TGG + chr2_1:65679571-65679590 Msa0218510:exon 50.0%
GCTTCAAAATGGCTCCACCT+GGG + chr2_1:65679572-65679591 Msa0218510:exon 50.0%
TGAAACCCAGAAAGCTTCCC+AGG - chr2_1:65679592-65679611 None:intergenic 50.0%
GAAGAACCTGACAGTGGTAC+TGG + chr2_1:65680744-65680763 Msa0218510:CDS 50.0%
! CAAGGTATTCTGCAGCACAG+TGG - chr2_1:65680864-65680883 None:intergenic 50.0%
TGCACGGACCCGAGTTTATT+TGG + chr2_1:65681066-65681085 Msa0218510:CDS 50.0%
CGGACCCGAGTTTATTTGGA+TGG + chr2_1:65681070-65681089 Msa0218510:CDS 50.0%
! GGATGGCCGATGATGATGTA+TGG + chr2_1:65681087-65681106 Msa0218510:CDS 50.0%
! GTATGGGAGTTTTCAGAGCC+CGG + chr2_1:65681104-65681123 Msa0218510:CDS 50.0%
! TATGGGAGTTTTCAGAGCCC+GGG + chr2_1:65681105-65681124 Msa0218510:CDS 50.0%
TCCAAAGAATGCTTCCACCC+GGG - chr2_1:65681125-65681144 None:intergenic 50.0%
AAACCACCACTCGGATTCTG+AGG - chr2_1:65681178-65681197 None:intergenic 50.0%
TATGGCACCTGCAAGGCTTT+CGG - chr2_1:65681274-65681293 None:intergenic 50.0%
TAGTGCTGCCGTTGATCAGA+GGG + chr2_1:65681392-65681411 Msa0218510:CDS 50.0%
TCAGACGACGATGATGCTTG+CGG - chr2_1:65681711-65681730 None:intergenic 50.0%
ACACTTTGCAGGCTCATCCT+TGG + chr2_1:65681971-65681990 Msa0218510:intron 50.0%
GAGATGCATGAGCACATGCA+TGG - chr2_1:65682056-65682075 None:intergenic 50.0%
AGCCGGATGTGTCAATGCTT+TGG + chr2_1:65682149-65682168 Msa0218510:CDS 50.0%
TGCTGTTGTGCGCGAAAATC+AGG + chr2_1:65682257-65682276 Msa0218510:CDS 50.0%
GCTGTTGTGCGCGAAAATCA+GGG + chr2_1:65682258-65682277 Msa0218510:CDS 50.0%
GCGCGAAAATCAGGGTTTGA+AGG + chr2_1:65682266-65682285 Msa0218510:CDS 50.0%
ACCAAATCTCACAGTGCACG+CGG + chr2_1:65682369-65682388 Msa0218510:CDS 50.0%
ACAGAACGATCAAGGGGTTC+AGG - chr2_1:65682459-65682478 None:intergenic 50.0%
GGGAAGGGAAGCGAGAAGAA+AGG - chr2_1:65679358-65679377 None:intergenic 55.0%
CTCCACCTGGGAAGCTTTCT+GGG + chr2_1:65679584-65679603 Msa0218510:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG - chr2_1:65679589-65679608 None:intergenic 55.0%
TTCGGACCTCAGAATCCGAG+TGG + chr2_1:65681169-65681188 Msa0218510:CDS 55.0%
ATGAGACCCGAAAGCCTTGC+AGG + chr2_1:65681264-65681283 Msa0218510:CDS 55.0%
ATGGCACCTGCAAGGCTTTC+GGG - chr2_1:65681273-65681292 None:intergenic 55.0%
!! AGGAAGCACTTGCCAGGTCT+TGG + chr2_1:65681306-65681325 Msa0218510:CDS 55.0%
AGCACTTGCCAGGTCTTGGT+CGG + chr2_1:65681310-65681329 Msa0218510:CDS 55.0%
TGATCAACGGCAGCACTAGC+AGG - chr2_1:65681390-65681409 None:intergenic 55.0%
CTAGTGCTGCCGTTGATCAG+AGG + chr2_1:65681391-65681410 Msa0218510:CDS 55.0%
GCTTGCGGATCAAGCGATGA+AGG - chr2_1:65681696-65681715 None:intergenic 55.0%
ATCGACTGCTCGAGGTGGTA+CGG + chr2_1:65682203-65682222 Msa0218510:CDS 55.0%
ACCGCGTGCACTGTGAGATT+TGG - chr2_1:65682373-65682392 None:intergenic 55.0%
CTCGACTGATGCTCTTGGTG+TGG - chr2_1:65682484-65682503 None:intergenic 55.0%
!! CACCAAGAGCATCAGTCGAG+CGG + chr2_1:65682484-65682503 Msa0218510:CDS 55.0%
!! ACCAAGAGCATCAGTCGAGC+GGG + chr2_1:65682485-65682504 Msa0218510:CDS 55.0%
GCTCCACCTGGGAAGCTTTC+TGG + chr2_1:65679583-65679602 Msa0218510:CDS 60.0%
!! GCCCGGGTGGAAGCATTCTT+TGG + chr2_1:65681121-65681140 Msa0218510:CDS 60.0%
TGAGGTCCGAATCCTAGCGC+CGG - chr2_1:65681160-65681179 None:intergenic 60.0%
GGACCTCAGAATCCGAGTGG+TGG + chr2_1:65681172-65681191 Msa0218510:CDS 60.0%
ACTTGCCAGGTCTTGGTCGG+TGG + chr2_1:65681313-65681332 Msa0218510:CDS 60.0%
GTCGATCGAGCAGGAACTGC+TGG - chr2_1:65682189-65682208 None:intergenic 60.0%
ACAGTGCACGCGGTTCTCCT+CGG + chr2_1:65682379-65682398 Msa0218510:CDS 60.0%
! GGGAGTTTTCAGAGCCCGGG+TGG + chr2_1:65681108-65681127 Msa0218510:CDS 65.0%
CCAGGTCTTGGTCGGTGGCA+GGG + chr2_1:65681318-65681337 Msa0218510:CDS 65.0%
CCTCGAGCAGTCGATCGAGC+AGG - chr2_1:65682198-65682217 None:intergenic 65.0%
CCTGCTCGATCGACTGCTCG+AGG + chr2_1:65682195-65682214 Msa0218510:CDS 65.0%
GCTCGATCGACTGCTCGAGG+TGG + chr2_1:65682198-65682217 Msa0218510:CDS 65.0%
ACTGCTCGAGGTGGTACGGC+TGG + chr2_1:65682207-65682226 Msa0218510:CDS 65.0%
GCCCGCTCGACTGATGCTCT+TGG - chr2_1:65682489-65682508 None:intergenic 65.0%
GCCAGGTCTTGGTCGGTGGC+AGG + chr2_1:65681317-65681336 Msa0218510:CDS 70.0%
CCCTGCCACCGACCAAGACC+TGG - chr2_1:65681321-65681340 None:intergenic 70.0%
GGTCTTGGTCGGTGGCAGGG+TGG + chr2_1:65681321-65681340 Msa0218510:CDS 70.0%
GGTCGGTGGCAGGGTGGACA+AGG + chr2_1:65681327-65681346 Msa0218510:CDS 70.0%
CGGTGGCAGGGTGGACAAGG+TGG + chr2_1:65681330-65681349 Msa0218510:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 65679324 65682940 65679324 ID=Msa0218510;Name=Msa0218510
chr2_1 mRNA 65679324 65682940 65679324 ID=Msa0218510-mRNA-1;Parent=Msa0218510;Name=Msa0218510-mRNA-1;_AED=0.05;_eAED=0.05;_QI=256|1|1|1|1|1|4|389|634
chr2_1 exon 65679324 65679629 65679324 ID=Msa0218510-mRNA-1:exon:18309;Parent=Msa0218510-mRNA-1
chr2_1 exon 65679840 65679909 65679840 ID=Msa0218510-mRNA-1:exon:18310;Parent=Msa0218510-mRNA-1
chr2_1 exon 65680679 65681893 65680679 ID=Msa0218510-mRNA-1:exon:18311;Parent=Msa0218510-mRNA-1
chr2_1 exon 65681982 65682940 65681982 ID=Msa0218510-mRNA-1:exon:18312;Parent=Msa0218510-mRNA-1
chr2_1 five_prime_UTR 65679324 65679579 65679324 ID=Msa0218510-mRNA-1:five_prime_utr;Parent=Msa0218510-mRNA-1
chr2_1 CDS 65679580 65679629 65679580 ID=Msa0218510-mRNA-1:cds;Parent=Msa0218510-mRNA-1
chr2_1 CDS 65679840 65679909 65679840 ID=Msa0218510-mRNA-1:cds;Parent=Msa0218510-mRNA-1
chr2_1 CDS 65680679 65681893 65680679 ID=Msa0218510-mRNA-1:cds;Parent=Msa0218510-mRNA-1
chr2_1 CDS 65681982 65682551 65681982 ID=Msa0218510-mRNA-1:cds;Parent=Msa0218510-mRNA-1
chr2_1 three_prime_UTR 65682552 65682940 65682552 ID=Msa0218510-mRNA-1:three_prime_utr;Parent=Msa0218510-mRNA-1
Gene Sequence

>Msa0218510

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGTCATCACTGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACTGTGTCAATTGATGGTGTCAATTTCCATCTTCACAAGTTTCCTCTTGTTTCAAAATGTGGAAAAATTGCTGGTACCTATGAAGAATCCAAGAATGCCTATGAGAAGAACCTGACAGTGGTACTGGAAGAATTTCCTGGGGGTCCTGAAATTTTCTTGATTGTTGCCAAATTCTGTTACGGGTTTCGAGTAGAGCTGACAGCGAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGACGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAACATAATGCGCAACTGGAAAGATTGTATTTTGGCTCTCCAAAGCTCTGAGCCTATTCTATCAAGAGCTGAAAATCTTCATTTAGTGGATAAGTGTTTAAACGCTCTATCTATGATGGCTTGCACGGACCCGAGTTTATTTGGATGGCCGATGATGATGTATGGGAGTTTTCAGAGCCCGGGTGGAAGCATTCTTTGGAATGGTATAAATACCGGCGCTAGGATTCGGACCTCAGAATCCGAGTGGTGGTTTGAAGACATCTCATATCTCAGTGTGAGATTGTTTGAGAGCCTTATTAAAACGATGCGAGCGAGAGGTATGAGACCCGAAAGCCTTGCAGGTGCCATAATGTATTATTCTAGGAAGCACTTGCCAGGTCTTGGTCGGTGGCAGGGTGGACAAGGTGGCAAAACAAGAACAGTTGCAAGCTTTAGTTTGACGCCTGCTAGTGCTGCCGTTGATCAGAGGGTTCTTTTGGAAAGCATTGAGAAACTTCTTCCTCAAAAGAAGGGAAAATCTTTTTGCCGTTTCTTACTCGGACTTCTTCGTGTAGCTTCGATATTGAATGTTGATCAAGCTTGCCAGGAATCTTTGGAGAGAAGAATAGGGATGCAACTGGAACTTGCAACATTAGATAGTCTTCTCATTCCTGCTTATGCAGATTCTGATGCATTGTATAACACAGATTGTATTGAACATATTGTCCATCATTTTGTACTTATGGAATCAAATTTAACTGCCTTTTCTCCTTCATCGCTTGATCCGCAAGCATCATCGTCGTCTGAATCGTTAAGGAAAGTTGCGAAGTTGATAGATAGCTATATTGGCGAAATTGCGTCTGATGTTAATTTAAAACCAGAAAAGTTACGTGCTCTTGCACAAGCCCTTCCTGAGTCATCGAGATCATTGCATGACGGACTCTACCGAGCACTAGACATATATTTCAAGGCTCATCCTTGGCTCTCTGATAACGAGAAAGAAGAAATATGCAACATCATTGATTACCAAAAACTCTCAATCCATGCATGTGCTCATGCATCTCAAAATGATAGGTTACCCCTCAGAGTTGTTCTTCAAGTCTTATTCTTTGAACAGCTGCATTTGAGAACAGCATTAGCCGGATGTGTCAATGCTTTGGATGGCGAAAGCGCTCCAGCAGTTCCTGCTCGATCGACTGCTCGAGGTGGTACGGCTGGAGAAATTGTACAAAGAAACGGATGGGTTGCTGTTGTGCGCGAAAATCAGGGTTTGAAGGTGGATATGGATAGAATGAGTACTAGAGTCGGAGAACTTGAAGAAGAATTCAGTAAAATAAAAAAGGAAATGAAAACCGTGACCAAATCTCACAGTGCACGCGGTTCTCCTCGGTTACTTGCAAGAAAACTCGGGTGCAAGCTTGTTCCTAGATTTTCAGATGCTCAACCTGAACCCCTTGATCGTTCTGTATCCACACCAAGAGCATCAGTCGAGCGGGCACGCCGTTCCCATAAGTCCAGACACACAGAAAGTTTTTCTTGA
Protein sequence

>Msa0218510

MAPPGKLSGFHLQGHHWFSDSGLPSDITVSIDGVNFHLHKFPLVSKCGKIAGTYEESKNAYEKNLTVVLEEFPGGPEIFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNIMRNWKDCILALQSSEPILSRAENLHLVDKCLNALSMMACTDPSLFGWPMMMYGSFQSPGGSILWNGINTGARIRTSESEWWFEDISYLSVRLFESLIKTMRARGMRPESLAGAIMYYSRKHLPGLGRWQGGQGGKTRTVASFSLTPASAAVDQRVLLESIEKLLPQKKGKSFCRFLLGLLRVASILNVDQACQESLERRIGMQLELATLDSLLIPAYADSDALYNTDCIEHIVHHFVLMESNLTAFSPSSLDPQASSSSESLRKVAKLIDSYIGEIASDVNLKPEKLRALAQALPESSRSLHDGLYRALDIYFKAHPWLSDNEKEEICNIIDYQKLSIHACAHASQNDRLPLRVVLQVLFFEQLHLRTALAGCVNALDGESAPAVPARSTARGGTAGEIVQRNGWVAVVRENQGLKVDMDRMSTRVGELEEEFSKIKKEMKTVTKSHSARGSPRLLARKLGCKLVPRFSDAQPEPLDRSVSTPRASVERARRSHKSRHTESFS*