AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0578050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0578050 MtrunA17_Chr2g0320691 95.781 640 21 2 1 640 1 634 0.0 1260
Msa0578050 MtrunA17_Chr2g0303541 45.161 620 301 11 6 609 9 605 0.0 549
Msa0578050 MtrunA17_Chr8g0374011 38.209 670 348 17 13 635 10 660 1.51e-146 440
Msa0578050 MtrunA17_Chr1g0199631 39.216 561 304 9 75 625 35 568 3.61e-134 405
Msa0578050 MtrunA17_Chr4g0050021 35.726 613 340 10 6 582 27 621 2.21e-130 398
Msa0578050 MtrunA17_Chr2g0285511 36.441 590 329 9 6 583 9 564 7.46e-129 392
Msa0578050 MtrunA17_Chr2g0279481 34.921 630 343 12 6 589 27 635 1.59e-117 365
Msa0578050 MtrunA17_Chr1g0181181 35.017 574 319 10 10 576 13 539 2.07e-115 356
Msa0578050 MtrunA17_Chr4g0010091 34.821 560 330 8 23 577 34 563 2.58e-111 347
Msa0578050 MtrunA17_Chr5g0401451 34.576 590 343 13 7 590 24 576 1.35e-106 335
Msa0578050 MtrunA17_Chr7g0245201 32.640 625 362 14 18 637 58 628 5.00e-102 324
Msa0578050 MtrunA17_Chr5g0401341 37.832 489 261 9 23 501 27 482 4.81e-96 308
Msa0578050 MtrunA17_Chr7g0268781 33.502 594 341 14 21 597 34 590 1.68e-95 306
Msa0578050 MtrunA17_Chr8g0382231 29.195 596 341 11 15 588 44 580 4.22e-93 300
Msa0578050 MtrunA17_Chr1g0195911 33.000 600 333 15 21 597 34 587 7.02e-92 296
Msa0578050 MtrunA17_Chr4g0053841 31.811 613 370 16 16 615 6 583 3.75e-91 294
Msa0578050 MtrunA17_Chr1g0207461 32.867 572 322 10 10 552 13 551 6.06e-90 291
Msa0578050 MtrunA17_Chr1g0150191 33.881 487 292 7 21 501 25 487 1.49e-89 290
Msa0578050 MtrunA17_Chr3g0108331 31.634 569 327 11 2 569 3 510 1.33e-86 281
Msa0578050 MtrunA17_Chr5g0432861 31.699 612 339 11 4 603 6 550 1.10e-82 271
Msa0578050 MtrunA17_Chr8g0367891 31.927 545 299 11 26 542 29 529 8.13e-81 267
Msa0578050 MtrunA17_Chr5g0412591 33.794 506 267 12 21 500 24 487 8.23e-79 261
Msa0578050 MtrunA17_Chr4g0076521 33.402 482 287 11 21 500 24 473 3.25e-78 260
Msa0578050 MtrunA17_Chr1g0191711 27.544 570 344 8 13 582 48 548 5.75e-77 255
Msa0578050 MtrunA17_Chr2g0290741 30.769 481 291 11 21 500 24 463 4.52e-64 221
Msa0578050 MtrunA17_Chr4g0023801 28.411 535 316 15 71 586 46 532 3.45e-54 194
Msa0578050 MtrunA17_Chr4g0003891 36.177 293 162 4 215 503 43 314 9.72e-54 190
Msa0578050 MtrunA17_Chr2g0332281 34.843 287 170 3 218 503 24 294 3.68e-48 174
Msa0578050 MtrunA17_Chr4g0037661 27.200 500 310 11 26 506 6 470 2.23e-47 175
Msa0578050 MtrunA17_Chr1g0163311 25.560 536 314 17 75 582 44 522 1.11e-45 170
Msa0578050 MtrunA17_Chr2g0280541 26.055 403 242 10 97 488 1 358 8.82e-32 128
Msa0578050 MtrunA17_Chr1g0206621 30.973 113 78 0 62 174 101 213 2.34e-15 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0578050 AT3G44820.1 62.727 660 214 13 1 640 1 648 0.0 803
Msa0578050 AT1G30440.1 44.833 600 303 10 6 589 9 596 8.28e-174 510
Msa0578050 AT5G03250.1 39.355 620 299 14 6 600 9 576 1.34e-140 422
Msa0578050 AT5G13600.1 38.926 596 298 16 10 588 13 559 4.97e-133 403
Msa0578050 AT5G66560.1 37.519 669 325 17 13 609 9 656 7.24e-132 402
Msa0578050 AT5G66560.4 40.000 570 291 13 73 609 66 617 2.43e-126 387
Msa0578050 AT5G66560.3 39.474 570 294 12 73 609 76 627 3.07e-126 387
Msa0578050 AT3G50840.1 37.438 601 313 13 17 609 10 555 3.90e-124 379
Msa0578050 AT5G64330.1 33.859 697 345 17 6 606 35 711 3.52e-121 377
Msa0578050 AT5G64330.2 35.656 617 288 14 6 527 35 637 8.24e-116 361
Msa0578050 AT3G50840.2 37.982 545 282 11 73 609 23 519 2.19e-115 355
Msa0578050 AT3G50840.3 37.982 545 282 11 73 609 23 519 2.19e-115 355
Msa0578050 AT5G03250.2 38.552 511 241 12 116 600 1 464 2.23e-110 341
Msa0578050 AT5G48800.1 34.206 573 323 9 23 587 41 567 1.06e-106 335
Msa0578050 AT5G64330.3 35.636 550 253 11 73 527 35 578 7.96e-105 330
Msa0578050 AT3G08660.1 34.039 567 302 12 18 577 31 532 7.07e-99 314
Msa0578050 AT3G08570.1 33.625 571 327 12 18 577 31 560 1.68e-98 314
Msa0578050 AT5G67385.1 32.374 556 334 10 7 559 9 525 5.88e-94 302
Msa0578050 AT3G08660.3 33.647 532 286 10 53 577 1 472 9.61e-94 299
Msa0578050 AT2G14820.2 34.035 570 319 14 1 550 4 536 4.13e-93 301
Msa0578050 AT2G14820.1 34.035 570 319 14 1 550 4 536 4.13e-93 301
Msa0578050 AT2G30520.1 32.130 554 323 8 16 568 23 524 2.03e-92 298
Msa0578050 AT1G03010.3 31.193 654 387 14 7 638 21 633 5.88e-91 295
Msa0578050 AT1G03010.2 31.250 640 379 13 21 638 7 607 8.66e-91 294
Msa0578050 AT1G03010.1 31.250 640 379 13 21 638 34 634 2.47e-90 293
Msa0578050 AT5G67385.4 33.000 500 301 8 63 559 12 480 1.18e-89 289
Msa0578050 AT5G67385.5 33.000 500 301 8 63 559 12 480 1.18e-89 289
Msa0578050 AT5G67385.2 33.000 500 301 8 63 559 12 480 1.18e-89 289
Msa0578050 AT5G67385.3 33.000 500 301 8 63 559 12 480 1.18e-89 289
Msa0578050 AT2G30520.2 33.939 495 282 7 75 568 26 476 3.36e-88 285
Msa0578050 AT2G30520.3 33.939 495 282 7 75 568 26 476 3.36e-88 285
Msa0578050 AT5G67440.3 34.639 485 283 7 23 505 27 479 3.15e-87 283
Msa0578050 AT5G67440.1 34.639 485 283 7 23 505 27 479 3.15e-87 283
Msa0578050 AT5G66560.2 36.308 493 240 13 13 452 9 480 5.11e-87 280
Msa0578050 AT5G10250.2 29.027 596 344 12 8 579 35 575 2.33e-84 277
Msa0578050 AT1G67900.5 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT1G67900.1 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT1G67900.3 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT1G67900.6 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT1G67900.2 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT1G67900.4 35.771 506 277 8 21 500 24 507 2.48e-84 277
Msa0578050 AT5G47800.3 34.167 480 281 9 24 500 27 474 7.82e-84 274
Msa0578050 AT5G47800.1 34.167 480 281 9 24 500 27 474 7.82e-84 274
Msa0578050 AT5G47800.4 34.167 480 281 9 24 500 27 474 7.82e-84 274
Msa0578050 AT5G67440.2 35.797 433 249 6 75 505 37 442 1.15e-82 270
Msa0578050 AT2G47860.3 29.712 626 370 14 21 609 35 627 1.37e-82 273
Msa0578050 AT2G47860.1 30.782 614 356 15 21 609 35 604 2.32e-82 272
Msa0578050 AT5G47800.2 34.167 480 281 9 24 500 140 587 2.37e-82 273
Msa0578050 AT5G10250.1 29.060 585 343 12 8 579 35 560 3.22e-81 268
Msa0578050 AT4G37590.1 33.271 535 299 15 1 527 4 488 1.90e-77 258
Msa0578050 AT4G31820.1 33.130 492 275 13 18 501 22 467 3.47e-77 257
Msa0578050 AT3G26490.1 31.311 511 291 9 21 500 24 505 8.61e-77 256
Msa0578050 AT3G26490.3 31.311 511 291 9 21 500 24 505 8.61e-77 256
Msa0578050 AT3G26490.4 31.311 511 291 9 21 500 24 505 8.61e-77 256
Msa0578050 AT2G23050.1 32.848 481 259 9 33 509 37 457 1.57e-76 253
Msa0578050 AT4G31820.2 33.484 442 248 10 68 501 11 414 1.49e-72 243
Msa0578050 AT3G49970.1 28.835 541 293 13 31 569 2 452 2.12e-65 224
Msa0578050 AT3G49970.2 28.835 541 293 13 31 569 2 452 2.12e-65 224
Msa0578050 AT3G08660.2 31.900 442 240 9 18 453 31 417 2.83e-65 221
Msa0578050 AT2G47860.5 29.480 519 308 11 116 609 1 486 3.70e-64 221
Msa0578050 AT2G47860.2 29.480 519 308 11 116 609 1 486 3.70e-64 221
Msa0578050 AT3G26490.2 29.806 463 265 9 21 452 24 457 1.61e-59 207
Msa0578050 AT2G23050.2 31.156 398 213 8 116 509 1 341 4.63e-57 197
Msa0578050 AT3G19850.1 28.348 448 271 6 71 501 50 464 6.92e-55 196
Msa0578050 AT2G47860.6 29.032 465 265 11 21 452 35 467 1.14e-53 191
Msa0578050 AT2G47860.4 30.464 453 251 12 21 452 35 444 2.68e-53 189
Msa0578050 AT1G52770.1 35.395 291 169 4 218 503 28 304 4.64e-50 181
Msa0578050 AT5G48130.1 24.791 597 371 17 17 596 33 568 2.22e-48 179
Msa0578050 AT1G50280.2 27.602 442 274 11 71 496 16 427 4.72e-48 176
Msa0578050 AT1G50280.1 27.602 442 274 11 71 496 49 460 9.28e-48 176
Msa0578050 AT3G03510.1 29.062 437 228 11 81 508 4 367 2.07e-47 173
Msa0578050 AT3G22104.1 27.290 524 286 11 70 580 45 486 8.53e-47 173
Msa0578050 AT3G15570.1 33.445 299 168 4 218 503 29 309 1.42e-46 171
Msa0578050 AT5G17580.1 27.000 500 291 11 25 508 7 448 6.40e-46 171
Msa0578050 AT5G17580.2 26.547 501 304 10 25 514 22 469 8.80e-46 171
Msa0578050 AT3G22104.4 26.122 490 268 10 103 580 4 411 1.96e-37 145
Msa0578050 AT3G22104.2 26.122 490 268 10 103 580 4 411 1.96e-37 145
Msa0578050 AT3G22104.3 26.122 490 268 10 103 580 4 411 1.96e-37 145
Msa0578050 AT3G49900.1 30.556 180 108 5 402 575 214 382 1.24e-13 74.3
Msa0578050 AT3G49900.2 29.508 183 109 5 402 575 214 385 4.37e-12 69.3

Find 170 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 4_1:+82484015 Msa0578050:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 4_1:+82484263 Msa0578050:intron
ATGGCACCTGCAAGGCTTTC+GGG 0.203178 4_1:-82485698 None:intergenic
GCAACAATCAAGAAAATTTC+AGG 0.212225 4_1:-82485212 None:intergenic
TTTCTGTGTGTCTGGACTTA+TGG 0.216589 4_1:-82486946 None:intergenic
TGCACGGACCCGAGTTTATT+TGG 0.228486 4_1:+82485494 Msa0578050:CDS
TTAGTATTTAACTGAGAATT+TGG 0.230331 4_1:+82483946 Msa0578050:five_prime_UTR
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 4_1:+82484016 Msa0578050:CDS
ACGAAACCCGTAACAGAATT+TGG 0.238252 4_1:-82485234 None:intergenic
GTTCTTCTCATAGGTATTCT+TGG 0.238932 4_1:-82485156 None:intergenic
TGCTGTTGTGCGCGAAAATC+AGG 0.238977 4_1:+82486685 Msa0578050:CDS
CAACTGGAAAGATTGTATTT+TGG 0.263796 4_1:+82485385 Msa0578050:CDS
CGTTGATCAGAGGGTTCTTT+TGG 0.270046 4_1:+82485829 Msa0578050:CDS
TCAGGGTTTGAAGGTGGATA+TGG 0.270583 4_1:+82486703 Msa0578050:CDS
GCCCGGGTGGAAGCATTCTT+TGG 0.292191 4_1:+82485549 Msa0578050:CDS
TCAAGCTTGCCAGGAATCTT+TGG 0.296925 4_1:+82485946 Msa0578050:CDS
TATGGCACCTGCAAGGCTTT+CGG 0.307178 4_1:-82485699 None:intergenic
ATTTGGTTAACAAAGTAAAA+AGG 0.310161 4_1:+82483963 Msa0578050:five_prime_UTR
AATATTGGAGATGCAAAGTT+TGG 0.317613 4_1:+82487347 Msa0578050:three_prime_UTR
ATGAAAATAAAGAGGAAATA+TGG 0.321948 4_1:-82486991 None:intergenic
TTCTGTGTGTCTGGACTTAT+GGG 0.322141 4_1:-82486945 None:intergenic
TTATTTCCTTTGCTTCAAAA+TGG 0.324437 4_1:+82483993 Msa0578050:exon
GCCAGGTCTTGGTCGGTGGC+AGG 0.331550 4_1:+82485745 Msa0578050:CDS
CGTATTCGTCCGTCATTTCA+AGG 0.342271 4_1:-82485307 None:intergenic
CTTTCTGGGTTTCATCTTCA+AGG 0.343380 4_1:+82484030 Msa0578050:CDS
AGCTGAAAATCTTCATTTAG+TGG 0.344621 4_1:+82485439 Msa0578050:CDS
GGTATTCTTGGATTCTTCAT+AGG 0.349120 4_1:-82485144 None:intergenic
GTGCCATAATGTATTATTCT+AGG 0.366832 4_1:+82485714 Msa0578050:CDS
TTCTTCTCTCCAAAGATTCC+TGG 0.369534 4_1:-82485955 None:intergenic
ATAGAATAGGCTCAGAGCTT+TGG 0.373664 4_1:-82485412 None:intergenic
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 4_1:+82484264 Msa0578050:intron
TTGTTGCCAAATTCTGTTAC+GGG 0.377436 4_1:+82485228 Msa0578050:CDS
AGGAAGCACTTGCCAGGTCT+TGG 0.387407 4_1:+82485734 Msa0578050:CDS
ATGAGACCCGAAAGCCTTGC+AGG 0.391213 4_1:+82485692 Msa0578050:CDS
GTCGATCGAGCAGGAACTGC+TGG 0.391220 4_1:-82486614 None:intergenic
TAAATACCGGCGCTAGGATT+CGG 0.393615 4_1:+82485579 Msa0578050:CDS
CTTCCTAGAATAATACATTA+TGG 0.393701 4_1:-82485717 None:intergenic
ACCGCGTGCACTGTGAGATT+TGG 0.397256 4_1:-82486798 None:intergenic
GAATCTTTGGAGAGAAGAAT+AGG 0.402865 4_1:+82485959 Msa0578050:CDS
ACAGAACGATCAAGGGGTTC+AGG 0.408900 4_1:-82486884 None:intergenic
GTGGTGCTGGAAGAATTTCC+TGG 0.413339 4_1:+82485185 Msa0578050:CDS
GTGATCAAAAGAAAGAATAT+TGG 0.417030 4_1:+82487332 Msa0578050:three_prime_UTR
GAAACTTCTTCCTCAAAAGA+AGG 0.424762 4_1:+82485862 Msa0578050:CDS
AGCACTAGACATATATTTCA+AGG 0.428043 4_1:+82486300 Msa0578050:CDS
CTTGGATTCTTCATAGGTAC+GGG 0.432098 4_1:-82485138 None:intergenic
GGTTGAGCATCTGAAAATCT+AGG 0.440908 4_1:-82486863 None:intergenic
CCAGGTCTTGGTCGGTGGCA+GGG 0.442080 4_1:+82485746 Msa0578050:CDS
TTTAAACGCTCTATCTATGA+TGG 0.443227 4_1:+82485469 Msa0578050:CDS
TTCTTTCCCTCATGTAAAGT+TGG 0.443597 4_1:+82487147 Msa0578050:three_prime_UTR
TGCTTCAAAATGGCTCCACC+TGG 0.443870 4_1:+82484003 Msa0578050:exon
ATTGTTGCCAAATTCTGTTA+CGG 0.444459 4_1:+82485227 Msa0578050:CDS
ATTCCATAAGTACAAAATGA+TGG 0.452056 4_1:-82486078 None:intergenic
CGGTTACTTGCAAGAAAACT+CGG 0.454375 4_1:+82486827 Msa0578050:CDS
CACTGTCAGGTTCTTCTCAT+AGG 0.456166 4_1:-82485165 None:intergenic
GATAGAGTAACTGAGATATT+CGG 0.456605 4_1:+82483888 Msa0578050:five_prime_UTR
CTTTGGAATGGTATAAATAC+CGG 0.458255 4_1:+82485566 Msa0578050:CDS
AATCTTTGGAGAGAAGAATA+GGG 0.459226 4_1:+82485960 Msa0578050:CDS
GATATTACTGTGTCAATTGA+TGG 0.459358 4_1:+82484296 Msa0578050:CDS
AGCCGGATGTGTCAATGCTT+TGG 0.460297 4_1:+82486577 Msa0578050:CDS
CTCATGCATCTCAAAATGAT+AGG 0.461872 4_1:+82486492 Msa0578050:CDS
TGATCAACGGCAGCACTAGC+AGG 0.464029 4_1:-82485815 None:intergenic
AGGAGAAAAGAAAAGTATGA+AGG 0.466174 4_1:-82483808 None:intergenic
AAGTTGATAGATAGCTATAT+TGG 0.467102 4_1:+82486178 Msa0578050:CDS
CATCGTCGTCTGAATCGTTA+AGG 0.468385 4_1:+82486146 Msa0578050:CDS
GAGAAGAATAGGGATGCAAC+TGG 0.469240 4_1:+82485970 Msa0578050:CDS
GAATGTTGATCAAGCTTGCC+AGG 0.470324 4_1:+82485937 Msa0578050:CDS
GGTCGGTGGCAGGGTGGACA+AGG 0.471322 4_1:+82485755 Msa0578050:CDS
GTCAGAAATTAAGTAAAGTC+TGG 0.471560 4_1:+82483910 Msa0578050:five_prime_UTR
TTTCCTCTTGTTTCAAGATG+TGG 0.472009 4_1:+82485108 Msa0578050:CDS
GGGAAGGGAAGCGAGAAGAA+AGG 0.472367 4_1:-82483787 None:intergenic
TCTTGGATTCTTCATAGGTA+CGG 0.472809 4_1:-82485139 None:intergenic
TATTCTAGGAAGCACTTGCC+AGG 0.478232 4_1:+82485728 Msa0578050:CDS
ATTCTTCCAGCACCACTGTC+AGG 0.483228 4_1:-82485178 None:intergenic
TTCATCTTCAAGGTCATCAC+TGG 0.484382 4_1:+82484040 Msa0578050:CDS
ACACTTTGCAGGCTCATCCT+TGG 0.485150 4_1:+82486399 Msa0578050:intron
CTTTACATGAGGGAAAGAAA+TGG 0.488916 4_1:-82487143 None:intergenic
ATGAATAAATGAAAATAAAG+AGG 0.488926 4_1:-82486999 None:intergenic
GGAGAAAAGAAAAGTATGAA+GGG 0.491413 4_1:-82483807 None:intergenic
GTGTGGATACAGAACGATCA+AGG 0.491533 4_1:-82486892 None:intergenic
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 4_1:-82484021 None:intergenic
AAAAGAAAAGTATGAAGGGA+AGG 0.494164 4_1:-82483803 None:intergenic
CGGACCCGAGTTTATTTGGA+TGG 0.499303 4_1:+82485498 Msa0578050:CDS
GGATGGCCGATGATGATGTA+TGG 0.500030 4_1:+82485515 Msa0578050:CDS
GCCCGCTCGACTGATGCTCT+TGG 0.504243 4_1:-82486914 None:intergenic
CCTATGTGTTGATAAATACA+AGG 0.512785 4_1:-82487238 None:intergenic
GCATCAGAATCTGCATAAGC+AGG 0.514844 4_1:-82486022 None:intergenic
ATCCAAAGCATTGACACATC+CGG 0.515020 4_1:-82486579 None:intergenic
GAAGAACCTGACAGTGGTGC+TGG 0.515078 4_1:+82485172 Msa0578050:CDS
AAGTGCATACCTTGTGAAGA+TGG 0.517267 4_1:-82484328 None:intergenic
GGAGAAATTGTACAAAGAAA+CGG 0.517355 4_1:+82486656 Msa0578050:CDS
TGGTGCTGGAAGAATTTCCT+GGG 0.521506 4_1:+82485186 Msa0578050:CDS
ATCAAGCGATGAAGGAGAAA+AGG 0.522933 4_1:-82486113 None:intergenic
CATTTGAGAACAGCATTAGC+CGG 0.524224 4_1:+82486560 Msa0578050:CDS
CCCTGCCACCGACCAAGACC+TGG 0.524414 4_1:-82485746 None:intergenic
CTAGTGCTGCCGTTGATCAG+AGG 0.525231 4_1:+82485819 Msa0578050:CDS
GCTTGCGGATCAAGCGATGA+AGG 0.528515 4_1:-82486121 None:intergenic
ACTGCTCGAGGTGGTACGGC+TGG 0.530026 4_1:+82486635 Msa0578050:CDS
ACCAAGAGCATCAGTCGAGC+GGG 0.538088 4_1:+82486913 Msa0578050:CDS
TTTCCACATCTTGAAACAAG+AGG 0.538173 4_1:-82485111 None:intergenic
AAGAGGAAACTGCAGTTTCA+CGG 0.540573 4_1:-82485094 None:intergenic
CCTTGTATTTATCAACACAT+AGG 0.544116 4_1:+82487238 Msa0578050:three_prime_UTR
ACAGTGCACGCGGTTCTCCT+CGG 0.546364 4_1:+82486807 Msa0578050:CDS
GCGCGAAAATCAGGGTTTGA+AGG 0.547646 4_1:+82486694 Msa0578050:CDS
TTCCAAAGAATGCTTCCACC+CGG 0.549351 4_1:-82485551 None:intergenic
GACTTGAAGAACAACTCTGA+GGG 0.551619 4_1:-82486519 None:intergenic
TAGTGCTGCCGTTGATCAGA+GGG 0.552150 4_1:+82485820 Msa0578050:CDS
TCTATCTATGATGGCTTGCA+CGG 0.554280 4_1:+82485478 Msa0578050:CDS
AAACTTCTTCCTCAAAAGAA+GGG 0.557294 4_1:+82485863 Msa0578050:CDS
GGTGGAAGCATTCTTTGGAA+TGG 0.559847 4_1:+82485554 Msa0578050:CDS
GCTGTTGTGCGCGAAAATCA+GGG 0.560005 4_1:+82486686 Msa0578050:CDS
AAAGAAAAGTATGAAGGGAA+GGG 0.563336 4_1:-82483802 None:intergenic
TCCAAAGAATGCTTCCACCC+GGG 0.565201 4_1:-82485550 None:intergenic
ATCGGCCATCCAAATAAACT+CGG 0.566431 4_1:-82485503 None:intergenic
ACTTGCCAGGTCTTGGTCGG+TGG 0.567054 4_1:+82485741 Msa0578050:CDS
AAACCACCACTCGGATTCTG+AGG 0.569410 4_1:-82485603 None:intergenic
ATGATCTCGATGACTCAGGA+AGG 0.570271 4_1:-82486258 None:intergenic
TGCAATGATCTCGATGACTC+AGG 0.571453 4_1:-82486262 None:intergenic
CTCGACTGATGCTCTTGGTG+TGG 0.573680 4_1:-82486909 None:intergenic
ATGGTATAAATACCGGCGCT+AGG 0.573781 4_1:+82485573 Msa0578050:CDS
CGAAAATCAGGGTTTGAAGG+TGG 0.575204 4_1:+82486697 Msa0578050:CDS
CCTCGAGCAGTCGATCGAGC+AGG 0.575521 4_1:-82486623 None:intergenic
ATCGACTGCTCGAGGTGGTA+CGG 0.579037 4_1:+82486631 Msa0578050:CDS
ATGACGGACGAATACGAAGA+AGG 0.579734 4_1:+82485314 Msa0578050:CDS
GAAGAAGTCCGAGTAAGAAA+CGG 0.583295 4_1:-82485898 None:intergenic
CGGTGGCAGGGTGGACAAGG+TGG 0.592330 4_1:+82485758 Msa0578050:CDS
TGTGGATACAGAACGATCAA+GGG 0.592917 4_1:-82486891 None:intergenic
TTCTCGTTATCAGAGAGCCA+AGG 0.599221 4_1:-82486416 None:intergenic
CAAAAGAACCCTCTGATCAA+CGG 0.599347 4_1:-82485828 None:intergenic
AGCACTTGCCAGGTCTTGGT+CGG 0.602120 4_1:+82485738 Msa0578050:CDS
CACCAAGAGCATCAGTCGAG+CGG 0.602703 4_1:+82486912 Msa0578050:CDS
TGAAATATATGTCTAGTGCT+CGG 0.602823 4_1:-82486297 None:intergenic
GCTTCAAAATGGCTCCACCT+GGG 0.603801 4_1:+82484004 Msa0578050:exon
GAGATGCATGAGCACATGCA+TGG 0.604027 4_1:-82486481 None:intergenic
TCTCAGTTACTCTATCATCA+TGG 0.604133 4_1:-82483881 None:intergenic
AATTGTACAAAGAAACGGAT+GGG 0.604670 4_1:+82486661 Msa0578050:CDS
TGCAGAATACCTTGAAATGA+CGG 0.605457 4_1:+82485298 Msa0578050:CDS
CCTGCTCGATCGACTGCTCG+AGG 0.610208 4_1:+82486623 Msa0578050:CDS
TGCACTGTGAGATTTGGTCA+CGG 0.613993 4_1:-82486792 None:intergenic
ATTAAAACGATGCGAGCGAG+AGG 0.617106 4_1:+82485668 Msa0578050:CDS
AAACTCCCATACATCATCAT+CGG 0.620072 4_1:-82485521 None:intergenic
GATAGAATGAGTACTAGAGT+CGG 0.620497 4_1:+82486725 Msa0578050:CDS
GATGGCCGATGATGATGTAT+GGG 0.621720 4_1:+82485516 Msa0578050:CDS
GGTCTTGGTCGGTGGCAGGG+TGG 0.626378 4_1:+82485749 Msa0578050:CDS
GGATGTGTCAATGCTTTGGA+TGG 0.630355 4_1:+82486581 Msa0578050:CDS
GGACCTCAGAATCCGAGTGG+TGG 0.632921 4_1:+82485600 Msa0578050:CDS
TGATCTCGATGACTCAGGAA+GGG 0.637384 4_1:-82486257 None:intergenic
GGTGCTGGAAGAATTTCCTG+GGG 0.637441 4_1:+82485187 Msa0578050:CDS
TGAGGTCCGAATCCTAGCGC+CGG 0.638429 4_1:-82485585 None:intergenic
GATGTCTTCAAACCACCACT+CGG 0.642289 4_1:-82485612 None:intergenic
AATACATTATGGCACCTGCA+AGG 0.648443 4_1:-82485706 None:intergenic
GGTTACTTGCAAGAAAACTC+GGG 0.650025 4_1:+82486828 Msa0578050:CDS
TCAGACGACGATGATGCTTG+CGG 0.656201 4_1:-82486136 None:intergenic
ATTGACACAGTAATATCACT+TGG 0.657084 4_1:-82484290 None:intergenic
TCGGCCATCCAAATAAACTC+GGG 0.657524 4_1:-82485502 None:intergenic
AAATTGTACAAAGAAACGGA+TGG 0.658614 4_1:+82486660 Msa0578050:CDS
AGACTTGAAGAACAACTCTG+AGG 0.663274 4_1:-82486520 None:intergenic
CTATGTGTTGATAAATACAA+GGG 0.664074 4_1:-82487237 None:intergenic
TGTCAATTTCCATCTTCACA+AGG 0.664191 4_1:+82484319 Msa0578050:CDS
ACCAAATCTCACAGTGCACG+CGG 0.665622 4_1:+82486797 Msa0578050:CDS
TGTGTCTGGACTTATGGGAA+CGG 0.677659 4_1:-82486940 None:intergenic
TCATCGAGATCATTGCATGA+CGG 0.684043 4_1:+82486268 Msa0578050:CDS
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 4_1:-82484018 None:intergenic
GTGGATACAGAACGATCAAG+GGG 0.686942 4_1:-82486890 None:intergenic
GCTCGATCGACTGCTCGAGG+TGG 0.696171 4_1:+82486626 Msa0578050:CDS
ACTTGAAGAACAACTCTGAG+GGG 0.701967 4_1:-82486518 None:intergenic
GTGCTGGAAGAATTTCCTGG+GGG 0.715345 4_1:+82485188 Msa0578050:CDS
TTCGGACCTCAGAATCCGAG+TGG 0.719040 4_1:+82485597 Msa0578050:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 4_1:-82485289 None:intergenic
ATACACATGAAAGTGTTGTG+TGG 0.736317 4_1:+82487024 Msa0578050:three_prime_UTR
CTATGAGAAGAACCTGACAG+TGG 0.738065 4_1:+82485166 Msa0578050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAAAGAAAGAAAA+AGG - chr4_1:82483831-82483850 None:intergenic 10.0%
!! CTTCTTTATTATTTATTTAT+TGG + chr4_1:82484087-82484106 Msa0578050:intron 10.0%
!!! ATTTTTTTTTCCTGTTTTTT+TGG + chr4_1:82484232-82484251 Msa0578050:intron 15.0%
!! TATTATGAATAGATTATTAG+TGG - chr4_1:82484382-82484401 None:intergenic 15.0%
!! TATTCATAATAATTTGATAG+TGG + chr4_1:82484391-82484410 Msa0578050:intron 15.0%
!! AATCAAAATAACAGTAAAAT+AGG - chr4_1:82484525-82484544 None:intergenic 15.0%
!!! AAAACTTTTTTACATCAATA+GGG - chr4_1:82484555-82484574 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG - chr4_1:82484556-82484575 None:intergenic 15.0%
!! AGAATTCAGTAAAATAAAAA+AGG + chr4_1:82486760-82486779 Msa0578050:CDS 15.0%
!! ATGAATAAATGAAAATAAAG+AGG - chr4_1:82487002-82487021 None:intergenic 15.0%
!! TTAGTATTTAACTGAGAATT+TGG + chr4_1:82483946-82483965 Msa0578050:five_prime_UTR 20.0%
!! ATTTGGTTAACAAAGTAAAA+AGG + chr4_1:82483963-82483982 Msa0578050:five_prime_UTR 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG - chr4_1:82484245-82484264 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG + chr4_1:82484245-82484264 Msa0578050:intron 20.0%
!! ACAAAAGAACAAAAATGAAT+AGG - chr4_1:82484459-82484478 None:intergenic 20.0%
!! GATATTGGAGAAAATTTATT+TGG + chr4_1:82484909-82484928 Msa0578050:intron 20.0%
!! ATGAAAATAAAGAGGAAATA+TGG - chr4_1:82486994-82487013 None:intergenic 20.0%
!!! GTTTAAATTTTTTTGTACGT+GGG + chr4_1:82487046-82487065 Msa0578050:three_prime_UTR 20.0%
!! GCTTAAATAAAAATGCATAA+TGG - chr4_1:82487101-82487120 None:intergenic 20.0%
!!! TTTCTTTGTACATTTTTCAT+TGG + chr4_1:82487173-82487192 Msa0578050:three_prime_UTR 20.0%
! TTATTTCCTTTGCTTCAAAA+TGG + chr4_1:82483993-82484012 Msa0578050:exon 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG + chr4_1:82484155-82484174 Msa0578050:intron 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG + chr4_1:82484249-82484268 Msa0578050:intron 25.0%
! TAGATTATTAGTGGAAGAAA+AGG - chr4_1:82484373-82484392 None:intergenic 25.0%
!!! TTGGGATTTTTTTTCTTTTG+TGG + chr4_1:82484422-82484441 Msa0578050:intron 25.0%
!!! TTTTTGACTCTTTACATTTG+CGG + chr4_1:82484668-82484687 Msa0578050:intron 25.0%
!!! AGGTGAATATTTTGAGATAT+TGG + chr4_1:82484894-82484913 Msa0578050:intron 25.0%
! ATTGGAGAAAATTTATTTGG+AGG + chr4_1:82484912-82484931 Msa0578050:intron 25.0%
! CACGTAATTTATCATATCAA+AGG - chr4_1:82485043-82485062 None:intergenic 25.0%
!!! AGTTGCGCATTATATTTTTA+TGG - chr4_1:82485370-82485389 None:intergenic 25.0%
! ATAAAAATATAATGCGCAAC+TGG + chr4_1:82485369-82485388 Msa0578050:CDS 25.0%
! CTTCCTAGAATAATACATTA+TGG - chr4_1:82485720-82485739 None:intergenic 25.0%
! ATTCCATAAGTACAAAATGA+TGG - chr4_1:82486081-82486100 None:intergenic 25.0%
!!! AAGTTGATAGATAGCTATAT+TGG + chr4_1:82486178-82486197 Msa0578050:CDS 25.0%
!!! GGTTTAAATTTTTTTGTACG+TGG + chr4_1:82487045-82487064 Msa0578050:three_prime_UTR 25.0%
! AAAAAACCAACTTTACATGA+GGG - chr4_1:82487156-82487175 None:intergenic 25.0%
!! CTATGTGTTGATAAATACAA+GGG - chr4_1:82487240-82487259 None:intergenic 25.0%
!! ATTTTAGACATCAATTGTGA+AGG + chr4_1:82487301-82487320 Msa0578050:three_prime_UTR 25.0%
! GTGATCAAAAGAAAGAATAT+TGG + chr4_1:82487332-82487351 Msa0578050:three_prime_UTR 25.0%
! AAAGAAAAGTATGAAGGGAA+GGG - chr4_1:82483805-82483824 None:intergenic 30.0%
! AAAAGAAAAGTATGAAGGGA+AGG - chr4_1:82483806-82483825 None:intergenic 30.0%
! GGAGAAAAGAAAAGTATGAA+GGG - chr4_1:82483810-82483829 None:intergenic 30.0%
! AGGAGAAAAGAAAAGTATGA+AGG - chr4_1:82483811-82483830 None:intergenic 30.0%
GATAGAGTAACTGAGATATT+CGG + chr4_1:82483888-82483907 Msa0578050:five_prime_UTR 30.0%
GTCAGAAATTAAGTAAAGTC+TGG + chr4_1:82483910-82483929 Msa0578050:five_prime_UTR 30.0%
TGGTTAACAAAGTAAAAAGG+AGG + chr4_1:82483966-82483985 Msa0578050:five_prime_UTR 30.0%
GGTTAACAAAGTAAAAAGGA+GGG + chr4_1:82483967-82483986 Msa0578050:five_prime_UTR 30.0%
ATTGACACAGTAATATCACT+TGG - chr4_1:82484293-82484312 None:intergenic 30.0%
GATATTACTGTGTCAATTGA+TGG + chr4_1:82484296-82484315 Msa0578050:CDS 30.0%
AGTAAAATAGGTCACAAAGT+AGG - chr4_1:82484513-82484532 None:intergenic 30.0%
TAATAAGTCACACGTATGAT+TGG - chr4_1:82484598-82484617 None:intergenic 30.0%
TTGTGATCTCTAGTTAATGA+GGG - chr4_1:82484737-82484756 None:intergenic 30.0%
ATTGTGATCTCTAGTTAATG+AGG - chr4_1:82484738-82484757 None:intergenic 30.0%
GTTATGTGGCTACAATTAAA+AGG + chr4_1:82484868-82484887 Msa0578050:intron 30.0%
TGGCTACAATTAAAAGGAAA+AGG + chr4_1:82484874-82484893 Msa0578050:intron 30.0%
ATATTTGCACGGTCAAATAA+GGG + chr4_1:82484970-82484989 Msa0578050:intron 30.0%
AATTACGTGTAATGTTGTCT+TGG + chr4_1:82485054-82485073 Msa0578050:intron 30.0%
GCAACAATCAAGAAAATTTC+AGG - chr4_1:82485215-82485234 None:intergenic 30.0%
ATTGTTGCCAAATTCTGTTA+CGG + chr4_1:82485227-82485246 Msa0578050:CDS 30.0%
! CAACTGGAAAGATTGTATTT+TGG + chr4_1:82485385-82485404 Msa0578050:CDS 30.0%
! TTTTCAGCTCTTGATAGAAT+AGG - chr4_1:82485428-82485447 None:intergenic 30.0%
AGCTGAAAATCTTCATTTAG+TGG + chr4_1:82485439-82485458 Msa0578050:CDS 30.0%
TTTAAACGCTCTATCTATGA+TGG + chr4_1:82485469-82485488 Msa0578050:CDS 30.0%
!! CTTTGGAATGGTATAAATAC+CGG + chr4_1:82485566-82485585 Msa0578050:CDS 30.0%
GTGCCATAATGTATTATTCT+AGG + chr4_1:82485714-82485733 Msa0578050:CDS 30.0%
AAACTTCTTCCTCAAAAGAA+GGG + chr4_1:82485863-82485882 Msa0578050:CDS 30.0%
! AAAGATTTTCCCTTCTTTTG+AGG - chr4_1:82485875-82485894 None:intergenic 30.0%
! AATCTTTGGAGAGAAGAATA+GGG + chr4_1:82485960-82485979 Msa0578050:CDS 30.0%
! TGTCCATCATTTTGTACTTA+TGG + chr4_1:82486075-82486094 Msa0578050:CDS 30.0%
TGAAATATATGTCTAGTGCT+CGG - chr4_1:82486300-82486319 None:intergenic 30.0%
AGCACTAGACATATATTTCA+AGG + chr4_1:82486300-82486319 Msa0578050:CDS 30.0%
GGAGAAATTGTACAAAGAAA+CGG + chr4_1:82486656-82486675 Msa0578050:CDS 30.0%
AAATTGTACAAAGAAACGGA+TGG + chr4_1:82486660-82486679 Msa0578050:CDS 30.0%
AATTGTACAAAGAAACGGAT+GGG + chr4_1:82486661-82486680 Msa0578050:CDS 30.0%
!!! AATTTTTTTGTACGTGGGAA+AGG + chr4_1:82487051-82487070 Msa0578050:three_prime_UTR 30.0%
!!! ATTTTTTTGTACGTGGGAAA+GGG + chr4_1:82487052-82487071 Msa0578050:three_prime_UTR 30.0%
GAAAAAACCAACTTTACATG+AGG - chr4_1:82487157-82487176 None:intergenic 30.0%
!!! ATTGTCTTGTTTTGTTTGTG+TGG + chr4_1:82487207-82487226 Msa0578050:three_prime_UTR 30.0%
!!! TTGTCTTGTTTTGTTTGTGT+GGG + chr4_1:82487208-82487227 Msa0578050:three_prime_UTR 30.0%
! CCTATGTGTTGATAAATACA+AGG - chr4_1:82487241-82487260 None:intergenic 30.0%
CCTTGTATTTATCAACACAT+AGG + chr4_1:82487238-82487257 Msa0578050:three_prime_UTR 30.0%
!! CATTCTTCATTTTGACATAC+TGG + chr4_1:82487263-82487282 Msa0578050:three_prime_UTR 30.0%
TCTCAGTTACTCTATCATCA+TGG - chr4_1:82483884-82483903 None:intergenic 35.0%
AGTTGCATGTATGTATGACT+TGG - chr4_1:82484184-82484203 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG + chr4_1:82484263-82484282 Msa0578050:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG + chr4_1:82484264-82484283 Msa0578050:intron 35.0%
TGTCAATTTCCATCTTCACA+AGG + chr4_1:82484319-82484338 Msa0578050:CDS 35.0%
!! TTTGATAGTGGAGTAGTTGT+TGG + chr4_1:82484403-82484422 Msa0578050:intron 35.0%
!! TTGATAGTGGAGTAGTTGTT+GGG + chr4_1:82484404-82484423 Msa0578050:intron 35.0%
! CAAAGTAGGAGCACATTAAT+TGG - chr4_1:82484499-82484518 None:intergenic 35.0%
!!! CTTCTGTTTTGGAAGTTATG+TGG + chr4_1:82484854-82484873 Msa0578050:intron 35.0%
GAGAAAATTTATTTGGAGGC+TGG + chr4_1:82484916-82484935 Msa0578050:intron 35.0%
TATTTGACCGTGCAAATATG+CGG - chr4_1:82484969-82484988 None:intergenic 35.0%
CATATTTGCACGGTCAAATA+AGG + chr4_1:82484969-82484988 Msa0578050:intron 35.0%
TATTTGCACGGTCAAATAAG+GGG + chr4_1:82484971-82484990 Msa0578050:intron 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG + chr4_1:82485108-82485127 Msa0578050:CDS 35.0%
TTTCCACATCTTGAAACAAG+AGG - chr4_1:82485114-82485133 None:intergenic 35.0%
TCTTGGATTCTTCATAGGTA+CGG - chr4_1:82485142-82485161 None:intergenic 35.0%
GGTATTCTTGGATTCTTCAT+AGG - chr4_1:82485147-82485166 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG - chr4_1:82485159-82485178 None:intergenic 35.0%
TTGTTGCCAAATTCTGTTAC+GGG + chr4_1:82485228-82485247 Msa0578050:CDS 35.0%
TGCAGAATACCTTGAAATGA+CGG + chr4_1:82485298-82485317 Msa0578050:CDS 35.0%
AAACTCCCATACATCATCAT+CGG - chr4_1:82485524-82485543 None:intergenic 35.0%
GAAACTTCTTCCTCAAAAGA+AGG + chr4_1:82485862-82485881 Msa0578050:CDS 35.0%
!! TCTTTTTGCCGTTTCTTACT+CGG + chr4_1:82485890-82485909 Msa0578050:CDS 35.0%
! GAATCTTTGGAGAGAAGAAT+AGG + chr4_1:82485959-82485978 Msa0578050:CDS 35.0%
!! ATGCATGGATTGAGAGTTTT+TGG - chr4_1:82486469-82486488 None:intergenic 35.0%
CTCATGCATCTCAAAATGAT+AGG + chr4_1:82486492-82486511 Msa0578050:CDS 35.0%
GATAGAATGAGTACTAGAGT+CGG + chr4_1:82486725-82486744 Msa0578050:CDS 35.0%
AGAAAAACTTTCTGTGTGTC+TGG - chr4_1:82486957-82486976 None:intergenic 35.0%
!! ATACACATGAAAGTGTTGTG+TGG + chr4_1:82487024-82487043 Msa0578050:three_prime_UTR 35.0%
CTTTACATGAGGGAAAGAAA+TGG - chr4_1:82487146-82487165 None:intergenic 35.0%
TTCTTTCCCTCATGTAAAGT+TGG + chr4_1:82487147-82487166 Msa0578050:three_prime_UTR 35.0%
!!! TGTCTTGTTTTGTTTGTGTG+GGG + chr4_1:82487209-82487228 Msa0578050:three_prime_UTR 35.0%
CTTTCTGGGTTTCATCTTCA+AGG + chr4_1:82484030-82484049 Msa0578050:CDS 40.0%
TTCATCTTCAAGGTCATCAC+TGG + chr4_1:82484040-82484059 Msa0578050:CDS 40.0%
AAGTGCATACCTTGTGAAGA+TGG - chr4_1:82484331-82484350 None:intergenic 40.0%
! GTTCGCTTGATCTTCTGTTT+TGG + chr4_1:82484843-82484862 Msa0578050:intron 40.0%
TGAATTCCCGCATATTTGCA+CGG + chr4_1:82484959-82484978 Msa0578050:intron 40.0%
ATTTGACCGTGCAAATATGC+GGG - chr4_1:82484968-82484987 None:intergenic 40.0%
TAAGGGGAATTTACGCGAAA+CGG + chr4_1:82484987-82485006 Msa0578050:intron 40.0%
AAGAGGAAACTGCAGTTTCA+CGG - chr4_1:82485097-82485116 None:intergenic 40.0%
CTTGGATTCTTCATAGGTAC+GGG - chr4_1:82485141-82485160 None:intergenic 40.0%
ACGAAACCCGTAACAGAATT+TGG - chr4_1:82485237-82485256 None:intergenic 40.0%
ATAGAATAGGCTCAGAGCTT+TGG - chr4_1:82485415-82485434 None:intergenic 40.0%
! TCTATCTATGATGGCTTGCA+CGG + chr4_1:82485478-82485497 Msa0578050:CDS 40.0%
ATCGGCCATCCAAATAAACT+CGG - chr4_1:82485506-82485525 None:intergenic 40.0%
!! TCGCTCGCATCGTTTTAATA+AGG - chr4_1:82485667-82485686 None:intergenic 40.0%
AATACATTATGGCACCTGCA+AGG - chr4_1:82485709-82485728 None:intergenic 40.0%
CAAAAGAACCCTCTGATCAA+CGG - chr4_1:82485831-82485850 None:intergenic 40.0%
GAAGAAGTCCGAGTAAGAAA+CGG - chr4_1:82485901-82485920 None:intergenic 40.0%
TTCTTCTCTCCAAAGATTCC+TGG - chr4_1:82485958-82485977 None:intergenic 40.0%
ATCAAGCGATGAAGGAGAAA+AGG - chr4_1:82486116-82486135 None:intergenic 40.0%
TCATCGAGATCATTGCATGA+CGG + chr4_1:82486268-82486287 Msa0578050:CDS 40.0%
ACTTGAAGAACAACTCTGAG+GGG - chr4_1:82486521-82486540 None:intergenic 40.0%
GACTTGAAGAACAACTCTGA+GGG - chr4_1:82486522-82486541 None:intergenic 40.0%
AGACTTGAAGAACAACTCTG+AGG - chr4_1:82486523-82486542 None:intergenic 40.0%
CATTTGAGAACAGCATTAGC+CGG + chr4_1:82486560-82486579 Msa0578050:CDS 40.0%
!! ATCCAAAGCATTGACACATC+CGG - chr4_1:82486582-82486601 None:intergenic 40.0%
! AGTTTTCTTGCAAGTAACCG+AGG - chr4_1:82486827-82486846 None:intergenic 40.0%
CGGTTACTTGCAAGAAAACT+CGG + chr4_1:82486827-82486846 Msa0578050:CDS 40.0%
GGTTACTTGCAAGAAAACTC+GGG + chr4_1:82486828-82486847 Msa0578050:CDS 40.0%
GGTTGAGCATCTGAAAATCT+AGG - chr4_1:82486866-82486885 None:intergenic 40.0%
TGTGGATACAGAACGATCAA+GGG - chr4_1:82486894-82486913 None:intergenic 40.0%
TTCTGTGTGTCTGGACTTAT+GGG - chr4_1:82486948-82486967 None:intergenic 40.0%
TTTCTGTGTGTCTGGACTTA+TGG - chr4_1:82486949-82486968 None:intergenic 40.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG - chr4_1:82484002-82484021 None:intergenic 45.0%
CACTGTCAGGTTCTTCTCAT+AGG - chr4_1:82485168-82485187 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG + chr4_1:82485166-82485185 Msa0578050:CDS 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG + chr4_1:82485186-82485205 Msa0578050:CDS 45.0%
AAGAAAATTTCAGGACCCCC+AGG - chr4_1:82485206-82485225 None:intergenic 45.0%
CGTATTCGTCCGTCATTTCA+AGG - chr4_1:82485310-82485329 None:intergenic 45.0%
!! ATGACGGACGAATACGAAGA+AGG + chr4_1:82485314-82485333 Msa0578050:CDS 45.0%
TCGGCCATCCAAATAAACTC+GGG - chr4_1:82485505-82485524 None:intergenic 45.0%
! GATGGCCGATGATGATGTAT+GGG + chr4_1:82485516-82485535 Msa0578050:CDS 45.0%
TTCCAAAGAATGCTTCCACC+CGG - chr4_1:82485554-82485573 None:intergenic 45.0%
!! GGTGGAAGCATTCTTTGGAA+TGG + chr4_1:82485554-82485573 Msa0578050:CDS 45.0%
ATGGTATAAATACCGGCGCT+AGG + chr4_1:82485573-82485592 Msa0578050:CDS 45.0%
TAAATACCGGCGCTAGGATT+CGG + chr4_1:82485579-82485598 Msa0578050:CDS 45.0%
GATGTCTTCAAACCACCACT+CGG - chr4_1:82485615-82485634 None:intergenic 45.0%
ATTAAAACGATGCGAGCGAG+AGG + chr4_1:82485668-82485687 Msa0578050:CDS 45.0%
!! TATTCTAGGAAGCACTTGCC+AGG + chr4_1:82485728-82485747 Msa0578050:CDS 45.0%
! CGTTGATCAGAGGGTTCTTT+TGG + chr4_1:82485829-82485848 Msa0578050:CDS 45.0%
GAATGTTGATCAAGCTTGCC+AGG + chr4_1:82485937-82485956 Msa0578050:CDS 45.0%
TCAAGCTTGCCAGGAATCTT+TGG + chr4_1:82485946-82485965 Msa0578050:CDS 45.0%
GAGAAGAATAGGGATGCAAC+TGG + chr4_1:82485970-82485989 Msa0578050:CDS 45.0%
GCATCAGAATCTGCATAAGC+AGG - chr4_1:82486025-82486044 None:intergenic 45.0%
CATCGTCGTCTGAATCGTTA+AGG + chr4_1:82486146-82486165 Msa0578050:CDS 45.0%
!! GCAAGAGCACGTAACTTTTC+TGG - chr4_1:82486232-82486251 None:intergenic 45.0%
TGATCTCGATGACTCAGGAA+GGG - chr4_1:82486260-82486279 None:intergenic 45.0%
ATGATCTCGATGACTCAGGA+AGG - chr4_1:82486261-82486280 None:intergenic 45.0%
TGCAATGATCTCGATGACTC+AGG - chr4_1:82486265-82486284 None:intergenic 45.0%
ATCGATCGAGAATGTAGCTC+AGG - chr4_1:82486335-82486354 None:intergenic 45.0%
GAATGCTTACGACACTTTGC+AGG + chr4_1:82486388-82486407 Msa0578050:intron 45.0%
TTCTCGTTATCAGAGAGCCA+AGG - chr4_1:82486419-82486438 None:intergenic 45.0%
! GGATGTGTCAATGCTTTGGA+TGG + chr4_1:82486581-82486600 Msa0578050:CDS 45.0%
CGAAAATCAGGGTTTGAAGG+TGG + chr4_1:82486697-82486716 Msa0578050:CDS 45.0%
TCAGGGTTTGAAGGTGGATA+TGG + chr4_1:82486703-82486722 Msa0578050:CDS 45.0%
! TGCACTGTGAGATTTGGTCA+CGG - chr4_1:82486795-82486814 None:intergenic 45.0%
GTGGATACAGAACGATCAAG+GGG - chr4_1:82486893-82486912 None:intergenic 45.0%
GTGTGGATACAGAACGATCA+AGG - chr4_1:82486895-82486914 None:intergenic 45.0%
TGTGTCTGGACTTATGGGAA+CGG - chr4_1:82486943-82486962 None:intergenic 45.0%
! TTTTGTACGTGGGAAAGGGA+AGG + chr4_1:82487056-82487075 Msa0578050:three_prime_UTR 45.0%
TGCTTCAAAATGGCTCCACC+TGG + chr4_1:82484003-82484022 Msa0578050:exon 50.0%
GCTTCAAAATGGCTCCACCT+GGG + chr4_1:82484004-82484023 Msa0578050:exon 50.0%
TGAAACCCAGAAAGCTTCCC+AGG - chr4_1:82484024-82484043 None:intergenic 50.0%
ATTCTTCCAGCACCACTGTC+AGG - chr4_1:82485181-82485200 None:intergenic 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG + chr4_1:82485185-82485204 Msa0578050:CDS 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG + chr4_1:82485187-82485206 Msa0578050:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG + chr4_1:82485188-82485207 Msa0578050:CDS 50.0%
! CAAGGTATTCTGCAGCACAG+TGG - chr4_1:82485292-82485311 None:intergenic 50.0%
TGCACGGACCCGAGTTTATT+TGG + chr4_1:82485494-82485513 Msa0578050:CDS 50.0%
CGGACCCGAGTTTATTTGGA+TGG + chr4_1:82485498-82485517 Msa0578050:CDS 50.0%
! GGATGGCCGATGATGATGTA+TGG + chr4_1:82485515-82485534 Msa0578050:CDS 50.0%
! GTATGGGAGTTTTCAGAGCC+CGG + chr4_1:82485532-82485551 Msa0578050:CDS 50.0%
! TATGGGAGTTTTCAGAGCCC+GGG + chr4_1:82485533-82485552 Msa0578050:CDS 50.0%
TCCAAAGAATGCTTCCACCC+GGG - chr4_1:82485553-82485572 None:intergenic 50.0%
AAACCACCACTCGGATTCTG+AGG - chr4_1:82485606-82485625 None:intergenic 50.0%
TATGGCACCTGCAAGGCTTT+CGG - chr4_1:82485702-82485721 None:intergenic 50.0%
TAGTGCTGCCGTTGATCAGA+GGG + chr4_1:82485820-82485839 Msa0578050:CDS 50.0%
TCAGACGACGATGATGCTTG+CGG - chr4_1:82486139-82486158 None:intergenic 50.0%
ACACTTTGCAGGCTCATCCT+TGG + chr4_1:82486399-82486418 Msa0578050:intron 50.0%
GAGATGCATGAGCACATGCA+TGG - chr4_1:82486484-82486503 None:intergenic 50.0%
AGCCGGATGTGTCAATGCTT+TGG + chr4_1:82486577-82486596 Msa0578050:CDS 50.0%
TGCTGTTGTGCGCGAAAATC+AGG + chr4_1:82486685-82486704 Msa0578050:CDS 50.0%
GCTGTTGTGCGCGAAAATCA+GGG + chr4_1:82486686-82486705 Msa0578050:CDS 50.0%
GCGCGAAAATCAGGGTTTGA+AGG + chr4_1:82486694-82486713 Msa0578050:CDS 50.0%
ACCAAATCTCACAGTGCACG+CGG + chr4_1:82486797-82486816 Msa0578050:CDS 50.0%
ACAGAACGATCAAGGGGTTC+AGG - chr4_1:82486887-82486906 None:intergenic 50.0%
GGGAAGGGAAGCGAGAAGAA+AGG - chr4_1:82483790-82483809 None:intergenic 55.0%
CTCCACCTGGGAAGCTTTCT+GGG + chr4_1:82484016-82484035 Msa0578050:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG - chr4_1:82484021-82484040 None:intergenic 55.0%
GAAGAACCTGACAGTGGTGC+TGG + chr4_1:82485172-82485191 Msa0578050:CDS 55.0%
TTCGGACCTCAGAATCCGAG+TGG + chr4_1:82485597-82485616 Msa0578050:CDS 55.0%
ATGAGACCCGAAAGCCTTGC+AGG + chr4_1:82485692-82485711 Msa0578050:CDS 55.0%
ATGGCACCTGCAAGGCTTTC+GGG - chr4_1:82485701-82485720 None:intergenic 55.0%
!! AGGAAGCACTTGCCAGGTCT+TGG + chr4_1:82485734-82485753 Msa0578050:CDS 55.0%
AGCACTTGCCAGGTCTTGGT+CGG + chr4_1:82485738-82485757 Msa0578050:CDS 55.0%
TGATCAACGGCAGCACTAGC+AGG - chr4_1:82485818-82485837 None:intergenic 55.0%
CTAGTGCTGCCGTTGATCAG+AGG + chr4_1:82485819-82485838 Msa0578050:CDS 55.0%
GCTTGCGGATCAAGCGATGA+AGG - chr4_1:82486124-82486143 None:intergenic 55.0%
ATCGACTGCTCGAGGTGGTA+CGG + chr4_1:82486631-82486650 Msa0578050:CDS 55.0%
ACCGCGTGCACTGTGAGATT+TGG - chr4_1:82486801-82486820 None:intergenic 55.0%
CTCGACTGATGCTCTTGGTG+TGG - chr4_1:82486912-82486931 None:intergenic 55.0%
!! CACCAAGAGCATCAGTCGAG+CGG + chr4_1:82486912-82486931 Msa0578050:CDS 55.0%
!! ACCAAGAGCATCAGTCGAGC+GGG + chr4_1:82486913-82486932 Msa0578050:CDS 55.0%
GCTCCACCTGGGAAGCTTTC+TGG + chr4_1:82484015-82484034 Msa0578050:CDS 60.0%
!! GCCCGGGTGGAAGCATTCTT+TGG + chr4_1:82485549-82485568 Msa0578050:CDS 60.0%
TGAGGTCCGAATCCTAGCGC+CGG - chr4_1:82485588-82485607 None:intergenic 60.0%
GGACCTCAGAATCCGAGTGG+TGG + chr4_1:82485600-82485619 Msa0578050:CDS 60.0%
ACTTGCCAGGTCTTGGTCGG+TGG + chr4_1:82485741-82485760 Msa0578050:CDS 60.0%
GTCGATCGAGCAGGAACTGC+TGG - chr4_1:82486617-82486636 None:intergenic 60.0%
ACAGTGCACGCGGTTCTCCT+CGG + chr4_1:82486807-82486826 Msa0578050:CDS 60.0%
! GGGAGTTTTCAGAGCCCGGG+TGG + chr4_1:82485536-82485555 Msa0578050:CDS 65.0%
CCAGGTCTTGGTCGGTGGCA+GGG + chr4_1:82485746-82485765 Msa0578050:CDS 65.0%
CCTCGAGCAGTCGATCGAGC+AGG - chr4_1:82486626-82486645 None:intergenic 65.0%
CCTGCTCGATCGACTGCTCG+AGG + chr4_1:82486623-82486642 Msa0578050:CDS 65.0%
GCTCGATCGACTGCTCGAGG+TGG + chr4_1:82486626-82486645 Msa0578050:CDS 65.0%
ACTGCTCGAGGTGGTACGGC+TGG + chr4_1:82486635-82486654 Msa0578050:CDS 65.0%
GCCCGCTCGACTGATGCTCT+TGG - chr4_1:82486917-82486936 None:intergenic 65.0%
GCCAGGTCTTGGTCGGTGGC+AGG + chr4_1:82485745-82485764 Msa0578050:CDS 70.0%
CCCTGCCACCGACCAAGACC+TGG - chr4_1:82485749-82485768 None:intergenic 70.0%
GGTCTTGGTCGGTGGCAGGG+TGG + chr4_1:82485749-82485768 Msa0578050:CDS 70.0%
GGTCGGTGGCAGGGTGGACA+AGG + chr4_1:82485755-82485774 Msa0578050:CDS 70.0%
CGGTGGCAGGGTGGACAAGG+TGG + chr4_1:82485758-82485777 Msa0578050:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 82483756 82487368 82483756 ID=Msa0578050;Name=Msa0578050
chr4_1 mRNA 82483756 82487368 82483756 ID=Msa0578050-mRNA-1;Parent=Msa0578050;Name=Msa0578050-mRNA-1;_AED=0.05;_eAED=0.05;_QI=256|0.66|0.75|1|0.66|0.75|4|389|640
chr4_1 exon 82483756 82484061 82483756 ID=Msa0578050-mRNA-1:exon:29830;Parent=Msa0578050-mRNA-1
chr4_1 exon 82484271 82484340 82484271 ID=Msa0578050-mRNA-1:exon:29831;Parent=Msa0578050-mRNA-1
chr4_1 exon 82485089 82486321 82485089 ID=Msa0578050-mRNA-1:exon:29832;Parent=Msa0578050-mRNA-1
chr4_1 exon 82486410 82487368 82486410 ID=Msa0578050-mRNA-1:exon:29833;Parent=Msa0578050-mRNA-1
chr4_1 five_prime_UTR 82483756 82484011 82483756 ID=Msa0578050-mRNA-1:five_prime_utr;Parent=Msa0578050-mRNA-1
chr4_1 CDS 82484012 82484061 82484012 ID=Msa0578050-mRNA-1:cds;Parent=Msa0578050-mRNA-1
chr4_1 CDS 82484271 82484340 82484271 ID=Msa0578050-mRNA-1:cds;Parent=Msa0578050-mRNA-1
chr4_1 CDS 82485089 82486321 82485089 ID=Msa0578050-mRNA-1:cds;Parent=Msa0578050-mRNA-1
chr4_1 CDS 82486410 82486979 82486410 ID=Msa0578050-mRNA-1:cds;Parent=Msa0578050-mRNA-1
chr4_1 three_prime_UTR 82486980 82487368 82486980 ID=Msa0578050-mRNA-1:three_prime_utr;Parent=Msa0578050-mRNA-1
Gene Sequence

>Msa0578050

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGTCATCACTGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACTGTGTCAATTGATGGTGTCAATTTCCATCTTCACAAGATATGCCGTGAAACTGCAGTTTCCTCTTGTTTCAAGATGTGGAAAAATGCCCGTACCTATGAAGAATCCAAGAATACCTATGAGAAGAACCTGACAGTGGTGCTGGAAGAATTTCCTGGGGGTCCTGAAATTTTCTTGATTGTTGCCAAATTCTGTTACGGGTTTCGTGTAGAGCTGACAGCGAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGACGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAATATAATGCGCAACTGGAAAGATTGTATTTTGGCTCTCCAAAGCTCTGAGCCTATTCTATCAAGAGCTGAAAATCTTCATTTAGTGGATAAGTGTTTAAACGCTCTATCTATGATGGCTTGCACGGACCCGAGTTTATTTGGATGGCCGATGATGATGTATGGGAGTTTTCAGAGCCCGGGTGGAAGCATTCTTTGGAATGGTATAAATACCGGCGCTAGGATTCGGACCTCAGAATCCGAGTGGTGGTTTGAAGACATCTCATATCTCAGTGTGAGATTGTTTGAGAGCCTTATTAAAACGATGCGAGCGAGAGGTATGAGACCCGAAAGCCTTGCAGGTGCCATAATGTATTATTCTAGGAAGCACTTGCCAGGTCTTGGTCGGTGGCAGGGTGGACAAGGTGGCAAAACAAGAACAGTTGCAAGCTTTAGTTTGACGCCTGCTAGTGCTGCCGTTGATCAGAGGGTTCTTTTGGAAAGCATTGAGAAACTTCTTCCTCAAAAGAAGGGAAAATCTTTTTGCCGTTTCTTACTCGGACTTCTTCGTGTAGCTTCGATATTGAATGTTGATCAAGCTTGCCAGGAATCTTTGGAGAGAAGAATAGGGATGCAACTGGAACTTGCAACATTAGATAGTCTTCTCATTCCTGCTTATGCAGATTCTGATGCATTGTATAACACAGATTGTATTGAACATATTGTCCATCATTTTGTACTTATGGAATCAAATTTAACTGCCTTTTCTCCTTCATCGCTTGATCCGCAAGCATCATCGTCGTCTGAATCGTTAAGGAAAGTTGCGAAGTTGATAGATAGCTATATTGGCGAAATTGCGTCTGATGTTAATTTAAAACCAGAAAAGTTACGTGCTCTTGCACAAGCCCTTCCTGAGTCATCGAGATCATTGCATGACGGACTCTACCGAGCACTAGACATATATTTCAAGGCTCATCCTTGGCTCTCTGATAACGAGAAAGAAGAAATATGCAACATCATTGATTACCAAAAACTCTCAATCCATGCATGTGCTCATGCATCTCAAAATGATAGGTTACCCCTCAGAGTTGTTCTTCAAGTCTTATTCTTTGAACAGCTGCATTTGAGAACAGCATTAGCCGGATGTGTCAATGCTTTGGATGGCGAAAGCGCTCCAGCAGTTCCTGCTCGATCGACTGCTCGAGGTGGTACGGCTGGAGAAATTGTACAAAGAAACGGATGGGTTGCTGTTGTGCGCGAAAATCAGGGTTTGAAGGTGGATATGGATAGAATGAGTACTAGAGTCGGAGAACTTGAAGAAGAATTCAGTAAAATAAAAAAGGAAATGAAAACCGTGACCAAATCTCACAGTGCACGCGGTTCTCCTCGGTTACTTGCAAGAAAACTCGGGTGCAAGCTTGTTCCTAGATTTTCAGATGCTCAACCTGAACCCCTTGATCGTTCTGTATCCACACCAAGAGCATCAGTCGAGCGGGCACGCCGTTCCCATAAGTCCAGACACACAGAAAGTTTTTCTTGA
Protein sequence

>Msa0578050

MAPPGKLSGFHLQGHHWFSDSGLPSDITVSIDGVNFHLHKICRETAVSSCFKMWKNARTYEESKNTYEKNLTVVLEEFPGGPEIFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNIMRNWKDCILALQSSEPILSRAENLHLVDKCLNALSMMACTDPSLFGWPMMMYGSFQSPGGSILWNGINTGARIRTSESEWWFEDISYLSVRLFESLIKTMRARGMRPESLAGAIMYYSRKHLPGLGRWQGGQGGKTRTVASFSLTPASAAVDQRVLLESIEKLLPQKKGKSFCRFLLGLLRVASILNVDQACQESLERRIGMQLELATLDSLLIPAYADSDALYNTDCIEHIVHHFVLMESNLTAFSPSSLDPQASSSSESLRKVAKLIDSYIGEIASDVNLKPEKLRALAQALPESSRSLHDGLYRALDIYFKAHPWLSDNEKEEICNIIDYQKLSIHACAHASQNDRLPLRVVLQVLFFEQLHLRTALAGCVNALDGESAPAVPARSTARGGTAGEIVQRNGWVAVVRENQGLKVDMDRMSTRVGELEEEFSKIKKEMKTVTKSHSARGSPRLLARKLGCKLVPRFSDAQPEPLDRSVSTPRASVERARRSHKSRHTESFS*