AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0218630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218630 MtrunA17_Chr2g0320691 97.792 634 14 0 1 634 1 634 0.0 1285
Msa0218630 MtrunA17_Chr2g0303541 46.809 611 298 10 6 603 9 605 0.0 570
Msa0218630 MtrunA17_Chr8g0374011 39.130 667 340 17 13 629 10 660 1.22e-151 452
Msa0218630 MtrunA17_Chr4g0050021 37.397 607 332 10 6 576 27 621 1.13e-139 422
Msa0218630 MtrunA17_Chr1g0199631 38.879 589 322 10 41 619 8 568 1.42e-138 416
Msa0218630 MtrunA17_Chr2g0285511 37.500 584 325 7 6 577 9 564 2.30e-137 414
Msa0218630 MtrunA17_Chr2g0279481 36.334 622 339 11 6 583 27 635 1.67e-126 389
Msa0218630 MtrunA17_Chr1g0181181 36.444 568 313 8 10 570 13 539 1.58e-125 382
Msa0218630 MtrunA17_Chr4g0010091 36.101 554 325 7 23 571 34 563 1.33e-120 371
Msa0218630 MtrunA17_Chr5g0401451 35.556 585 338 13 7 584 24 576 3.46e-115 357
Msa0218630 MtrunA17_Chr7g0245201 33.710 620 356 14 18 631 58 628 1.49e-109 343
Msa0218630 MtrunA17_Chr7g0268781 34.524 588 337 12 21 591 34 590 3.30e-105 332
Msa0218630 MtrunA17_Chr8g0382231 30.678 590 334 10 15 582 44 580 1.78e-102 325
Msa0218630 MtrunA17_Chr1g0195911 34.175 594 328 13 21 591 34 587 2.78e-102 324
Msa0218630 MtrunA17_Chr5g0401341 38.716 483 259 9 23 495 27 482 9.23e-102 323
Msa0218630 MtrunA17_Chr4g0053841 32.949 607 365 15 16 609 6 583 6.63e-101 319
Msa0218630 MtrunA17_Chr1g0207461 33.569 566 320 10 10 546 13 551 1.80e-96 308
Msa0218630 MtrunA17_Chr1g0150191 34.719 481 290 6 21 495 25 487 2.77e-95 305
Msa0218630 MtrunA17_Chr3g0108331 32.149 563 326 10 2 563 3 510 2.32e-91 293
Msa0218630 MtrunA17_Chr5g0432861 33.114 607 331 12 4 597 6 550 4.99e-88 285
Msa0218630 MtrunA17_Chr8g0367891 33.024 539 295 10 26 536 29 529 9.73e-88 285
Msa0218630 MtrunA17_Chr4g0076521 34.874 476 282 9 21 494 24 473 6.68e-87 283
Msa0218630 MtrunA17_Chr5g0412591 34.800 500 264 10 21 494 24 487 4.17e-86 281
Msa0218630 MtrunA17_Chr1g0191711 28.445 566 338 8 13 576 48 548 2.48e-81 266
Msa0218630 MtrunA17_Chr2g0290741 32.000 475 287 9 21 494 24 463 1.47e-72 244
Msa0218630 MtrunA17_Chr4g0023801 27.883 581 349 18 20 580 2 532 1.04e-55 198
Msa0218630 MtrunA17_Chr4g0003891 36.177 293 162 4 209 497 43 314 5.36e-54 191
Msa0218630 MtrunA17_Chr4g0037661 27.475 495 309 10 26 500 6 470 2.19e-51 186
Msa0218630 MtrunA17_Chr1g0163311 25.308 569 334 18 36 576 17 522 4.62e-49 179
Msa0218630 MtrunA17_Chr2g0332281 34.843 287 170 3 212 497 24 294 3.16e-48 174
Msa0218630 MtrunA17_Chr2g0280541 26.055 403 242 10 91 482 1 358 3.11e-31 127
Msa0218630 MtrunA17_Chr1g0206621 33.333 138 92 0 31 168 76 213 3.28e-23 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218630 AT3G44820.1 63.914 654 210 11 1 634 1 648 0.0 825
Msa0218630 AT1G30440.1 45.455 594 302 7 6 583 9 596 0.0 531
Msa0218630 AT5G03250.1 40.820 610 298 12 6 594 9 576 5.75e-150 446
Msa0218630 AT5G13600.1 40.375 587 296 15 10 582 13 559 4.96e-141 423
Msa0218630 AT5G66560.1 37.838 666 321 15 13 603 9 656 2.48e-138 419
Msa0218630 AT5G64330.1 34.493 690 344 14 6 600 35 711 1.54e-129 399
Msa0218630 AT3G50840.1 37.856 597 310 13 17 603 10 555 8.40e-129 391
Msa0218630 AT5G66560.3 37.054 645 313 14 34 603 1 627 1.09e-128 393
Msa0218630 AT5G66560.4 40.000 570 291 13 67 603 66 617 9.55e-127 388
Msa0218630 AT5G64330.2 36.498 611 285 12 6 521 35 637 2.27e-124 383
Msa0218630 AT5G48800.1 35.802 567 316 9 23 581 41 567 1.05e-116 361
Msa0218630 AT3G50840.2 37.798 545 283 11 67 603 23 519 8.71e-115 354
Msa0218630 AT3G50840.3 37.798 545 283 11 67 603 23 519 8.71e-115 354
Msa0218630 AT5G03250.2 38.552 511 241 12 110 594 1 464 7.83e-110 339
Msa0218630 AT5G64330.3 35.456 581 272 12 36 521 6 578 1.61e-109 342
Msa0218630 AT3G08570.1 34.574 564 325 9 18 571 31 560 1.07e-107 338
Msa0218630 AT3G08660.1 34.938 561 299 11 18 571 31 532 1.31e-106 334
Msa0218630 AT5G67385.1 33.333 549 332 8 7 553 9 525 3.83e-103 326
Msa0218630 AT1G03010.3 33.025 648 377 14 7 632 21 633 1.02e-101 323
Msa0218630 AT1G03010.2 33.123 634 369 13 21 632 7 607 1.71e-101 322
Msa0218630 AT1G03010.1 33.123 634 369 13 21 632 34 634 5.10e-101 321
Msa0218630 AT2G14820.2 34.629 566 315 13 1 544 4 536 3.16e-100 319
Msa0218630 AT2G14820.1 34.629 566 315 13 1 544 4 536 3.16e-100 319
Msa0218630 AT2G30520.1 33.394 548 318 8 16 562 23 524 7.64e-97 309
Msa0218630 AT3G08660.3 33.962 530 283 10 49 571 3 472 4.38e-95 302
Msa0218630 AT5G10250.2 30.678 590 336 11 8 573 35 575 1.97e-93 301
Msa0218630 AT5G66560.2 36.735 490 236 11 13 446 9 480 4.11e-93 296
Msa0218630 AT2G47860.1 31.743 608 352 13 21 603 35 604 1.34e-92 299
Msa0218630 AT5G67385.4 32.812 512 310 8 44 553 1 480 2.71e-92 296
Msa0218630 AT5G67385.5 32.812 512 310 8 44 553 1 480 2.71e-92 296
Msa0218630 AT5G67385.2 32.812 512 310 8 44 553 1 480 2.71e-92 296
Msa0218630 AT5G67385.3 32.812 512 310 8 44 553 1 480 2.71e-92 296
Msa0218630 AT5G67440.3 35.611 483 275 8 23 499 27 479 3.37e-92 296
Msa0218630 AT5G67440.1 35.611 483 275 8 23 499 27 479 3.37e-92 296
Msa0218630 AT1G67900.5 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT1G67900.1 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT1G67900.3 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT1G67900.6 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT1G67900.2 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT1G67900.4 37.000 500 273 7 21 494 24 507 1.64e-91 296
Msa0218630 AT5G10250.1 30.743 579 335 11 8 573 35 560 2.37e-90 292
Msa0218630 AT2G30520.2 33.205 521 301 8 43 562 2 476 7.32e-88 284
Msa0218630 AT2G30520.3 33.205 521 301 8 43 562 2 476 7.32e-88 284
Msa0218630 AT5G47800.3 34.810 474 280 8 24 494 27 474 9.29e-88 284
Msa0218630 AT5G47800.1 34.810 474 280 8 24 494 27 474 9.29e-88 284
Msa0218630 AT5G47800.4 34.810 474 280 8 24 494 27 474 9.29e-88 284
Msa0218630 AT2G47860.3 30.586 631 352 14 21 603 35 627 2.49e-87 286
Msa0218630 AT5G47800.2 34.810 474 280 8 24 494 140 587 5.04e-86 283
Msa0218630 AT5G67440.2 35.043 468 268 8 38 499 5 442 8.06e-86 278
Msa0218630 AT4G31820.1 34.086 487 271 12 18 495 22 467 1.95e-84 276
Msa0218630 AT4G37590.1 33.826 541 296 14 1 529 4 494 2.28e-83 273
Msa0218630 AT2G23050.1 34.100 478 251 9 33 503 37 457 5.48e-83 269
Msa0218630 AT3G26490.1 31.391 532 306 9 21 520 24 528 1.71e-80 266
Msa0218630 AT3G26490.3 31.391 532 306 9 21 520 24 528 1.71e-80 266
Msa0218630 AT3G26490.4 31.391 532 306 9 21 520 24 528 1.71e-80 266
Msa0218630 AT3G49970.1 30.093 535 288 12 31 563 2 452 1.02e-74 248
Msa0218630 AT3G49970.2 30.093 535 288 12 31 563 2 452 1.02e-74 248
Msa0218630 AT4G31820.2 33.484 442 248 10 62 495 11 414 2.79e-73 245
Msa0218630 AT3G08660.2 33.028 436 237 8 18 447 31 417 1.18e-72 240
Msa0218630 AT2G47860.5 29.480 519 308 11 110 603 1 486 1.79e-64 221
Msa0218630 AT2G47860.2 29.480 519 308 11 110 603 1 486 1.79e-64 221
Msa0218630 AT2G47860.4 31.767 447 247 10 21 446 35 444 2.81e-63 216
Msa0218630 AT3G26490.2 29.913 458 265 7 21 446 24 457 5.72e-63 217
Msa0218630 AT2G47860.6 30.213 470 247 11 21 446 35 467 2.31e-58 203
Msa0218630 AT2G23050.2 31.156 398 213 8 110 503 1 341 2.77e-57 197
Msa0218630 AT3G19850.1 28.319 452 274 6 65 499 50 468 4.27e-55 197
Msa0218630 AT5G48130.1 25.297 589 372 16 17 590 33 568 1.29e-51 188
Msa0218630 AT1G52770.1 35.395 291 169 4 212 497 28 304 1.88e-50 182
Msa0218630 AT5G17580.1 27.823 496 298 10 25 508 7 454 2.67e-50 183
Msa0218630 AT5G17580.2 27.823 496 298 10 25 508 22 469 3.09e-50 183
Msa0218630 AT1G50280.2 27.602 442 274 11 65 490 16 427 2.20e-48 177
Msa0218630 AT3G22104.1 26.950 564 315 13 25 574 6 486 2.34e-48 177
Msa0218630 AT1G50280.1 27.602 442 274 11 65 490 49 460 3.01e-48 177
Msa0218630 AT3G15570.1 33.445 299 168 4 212 497 29 309 6.53e-47 172
Msa0218630 AT3G03510.1 29.062 437 228 11 75 502 4 367 1.76e-46 171
Msa0218630 AT3G22104.4 26.122 490 268 10 97 574 4 411 1.99e-37 145
Msa0218630 AT3G22104.2 26.122 490 268 10 97 574 4 411 1.99e-37 145
Msa0218630 AT3G22104.3 26.122 490 268 10 97 574 4 411 1.99e-37 145
Msa0218630 AT3G49900.1 30.556 180 108 5 396 569 214 382 1.26e-13 74.3
Msa0218630 AT3G49900.2 29.508 183 109 5 396 569 214 385 3.91e-12 69.7

Find 165 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 2_1:+65899488 Msa0218630:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 2_1:+65899747 Msa0218630:intron
ATGGCACCTGCAAGGCTTTC+GGG 0.203178 2_1:-65901157 None:intergenic
GCCACAATCAAGAATGTTTC+AGG 0.210799 2_1:-65900671 None:intergenic
TTTCTGTGTGTCTGGACTTA+TGG 0.216589 2_1:-65902405 None:intergenic
TGCACGGACCCGAGTTTATT+TGG 0.228486 2_1:+65900953 Msa0218630:CDS
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 2_1:+65899489 Msa0218630:CDS
GTTCTTCTCATAGGTATTCT+TGG 0.237719 2_1:-65900615 None:intergenic
TGCTGTTGTGCGCGAAAATC+AGG 0.238977 2_1:+65902144 Msa0218630:CDS
ACGAAACCCGTAACAGAATT+TGG 0.245072 2_1:-65900693 None:intergenic
CAACTGGAAAGATTGTATTT+TGG 0.263796 2_1:+65900844 Msa0218630:CDS
CGTTGATCAGAGGGTTCTTT+TGG 0.270046 2_1:+65901288 Msa0218630:CDS
TCAGGGTTTGAAGGTGGATA+TGG 0.270583 2_1:+65902162 Msa0218630:CDS
TCAAGCTTGCCAGGAATCTT+TGG 0.296925 2_1:+65901405 Msa0218630:CDS
TATGGCACCTGCAAGGCTTT+CGG 0.307178 2_1:-65901158 None:intergenic
GCCCTGGTGGAAGCATTCTT+TGG 0.309477 2_1:+65901008 Msa0218630:CDS
AATATTGGAGATGCAAAGTT+TGG 0.317613 2_1:+65902806 Msa0218630:three_prime_UTR
TTGTTTCCTTTGCTTCAAAA+TGG 0.321559 2_1:+65899466 Msa0218630:exon
ATGAAAATAAAGAGGAAATA+TGG 0.321948 2_1:-65902450 None:intergenic
TTCTGTGTGTCTGGACTTAT+GGG 0.322141 2_1:-65902404 None:intergenic
GCCAGGTCTTGGTCGGTGGC+AGG 0.331550 2_1:+65901204 Msa0218630:CDS
CGTATTCGTCCGTCATTTCA+AGG 0.342271 2_1:-65900766 None:intergenic
CTTTCTGGGTTTCATCTTCA+AGG 0.343380 2_1:+65899503 Msa0218630:CDS
AGCTGAAAATCTTCATTTAG+TGG 0.344621 2_1:+65900898 Msa0218630:CDS
ATAGAATAGGCTCGGAGCTT+TGG 0.362431 2_1:-65900871 None:intergenic
GGTATTCTTGGAATCTTCAT+AGG 0.366317 2_1:-65900603 None:intergenic
GTGCCATAATGTATTATTCT+AGG 0.366832 2_1:+65901173 Msa0218630:CDS
TTCTTCTCTCCAAAGATTCC+TGG 0.369534 2_1:-65901414 None:intergenic
TTGTGGCCAAATTCTGTTAC+GGG 0.375010 2_1:+65900687 Msa0218630:CDS
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 2_1:+65899748 Msa0218630:intron
AGGAAGCACTTGCCAGGTCT+TGG 0.387407 2_1:+65901193 Msa0218630:CDS
ATGAGACCCGAAAGCCTTGC+AGG 0.391213 2_1:+65901151 Msa0218630:CDS
GTCGATCGAGCAGGAACTGC+TGG 0.391220 2_1:-65902073 None:intergenic
TGATAGAGTAATTAGTTATT+CGG 0.391435 2_1:+65899355 Msa0218630:five_prime_UTR
TAAATACCGGCGCTAGGATT+CGG 0.393615 2_1:+65901038 Msa0218630:CDS
CTTCCTAGAATAATACATTA+TGG 0.393701 2_1:-65901176 None:intergenic
GAATCTTTGGAGAGAAGAAT+AGG 0.402865 2_1:+65901418 Msa0218630:CDS
ACCACGTGCACTGTGAGATT+TGG 0.408727 2_1:-65902257 None:intergenic
ACAGAACGATCAAGGGGTTC+AGG 0.408900 2_1:-65902343 None:intergenic
GTGGTGCTGGAAGAATTTCC+TGG 0.413339 2_1:+65900644 Msa0218630:CDS
GTGATCAAAAGAAAGAATAT+TGG 0.417030 2_1:+65902791 Msa0218630:three_prime_UTR
GAAACTTCTTCCTCAAAAGA+AGG 0.424762 2_1:+65901321 Msa0218630:CDS
AGCACTAGACATATATTTCA+AGG 0.428043 2_1:+65901759 Msa0218630:CDS
GGTTGAGCATCTGAAAATCT+AGG 0.440908 2_1:-65902322 None:intergenic
CCAGGTCTTGGTCGGTGGCA+GGG 0.442080 2_1:+65901205 Msa0218630:CDS
TTTAAACGCTCTATCTATGA+TGG 0.443227 2_1:+65900928 Msa0218630:CDS
TTCTTTCCCTCATGTAAAGT+TGG 0.443597 2_1:+65902606 Msa0218630:three_prime_UTR
TGCTTCAAAATGGCTCCACC+TGG 0.443870 2_1:+65899476 Msa0218630:exon
ATTCCATAAGTACAAAATGA+TGG 0.452056 2_1:-65901537 None:intergenic
CGGTTACTTGCAAGAAAACT+CGG 0.454375 2_1:+65902286 Msa0218630:CDS
CACTGTCAGGTTCTTCTCAT+AGG 0.456166 2_1:-65900624 None:intergenic
CTTTGGAATGGTATAAATAC+CGG 0.458255 2_1:+65901025 Msa0218630:CDS
AATCTTTGGAGAGAAGAATA+GGG 0.459226 2_1:+65901419 Msa0218630:CDS
GATATTACTGTGTCAATTGA+TGG 0.459358 2_1:+65899780 Msa0218630:CDS
ATTGTGGCCAAATTCTGTTA+CGG 0.459464 2_1:+65900686 Msa0218630:CDS
GCACTTTGCAGGCTCATCCT+TGG 0.459887 2_1:+65901858 Msa0218630:intron
AGCCGGATGTGTCAATGCTT+TGG 0.460297 2_1:+65902036 Msa0218630:CDS
CTCATGCATCTCAAAATGAT+AGG 0.461872 2_1:+65901951 Msa0218630:CDS
TGATCAACGGCAGCACTAGC+AGG 0.464029 2_1:-65901274 None:intergenic
CTTGGAATCTTCATAGGTAC+GGG 0.466842 2_1:-65900597 None:intergenic
AAGTTGATAGATAGCTATAT+TGG 0.467102 2_1:+65901637 Msa0218630:CDS
CATCGTCGTCTGAATCGTTA+AGG 0.468385 2_1:+65901605 Msa0218630:CDS
GAGAAGAATAGGGATGCAAC+TGG 0.469240 2_1:+65901429 Msa0218630:CDS
GAATGTTGATCAAGCTTGCC+AGG 0.470324 2_1:+65901396 Msa0218630:CDS
GGTCGGTGGCAGGGTGGACA+AGG 0.471322 2_1:+65901214 Msa0218630:CDS
TTTCCTCTTGTTTCAAGATG+TGG 0.472009 2_1:+65900566 Msa0218630:CDS
TATTCTAGGAAGCACTTGCC+AGG 0.478232 2_1:+65901187 Msa0218630:CDS
ATTCTTCCAGCACCACTGTC+AGG 0.483228 2_1:-65900637 None:intergenic
TTCATCTTCAAGGTCATCAC+TGG 0.484382 2_1:+65899513 Msa0218630:CDS
GTATGAATGAAGGGAAGAGA+AGG 0.487617 2_1:-65899266 None:intergenic
CTTTACATGAGGGAAAGAAA+TGG 0.488916 2_1:-65902602 None:intergenic
ATGAATAAATGAAAATAAAG+AGG 0.488926 2_1:-65902458 None:intergenic
GTGTGGATACAGAACGATCA+AGG 0.491533 2_1:-65902351 None:intergenic
GGGAAGAGAAGGGAGAAGAA+AGG 0.491869 2_1:-65899255 None:intergenic
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 2_1:-65899494 None:intergenic
TCTTGGAATCTTCATAGGTA+CGG 0.494586 2_1:-65900598 None:intergenic
CGGACCCGAGTTTATTTGGA+TGG 0.499303 2_1:+65900957 Msa0218630:CDS
TATGAATGAAGGGAAGAGAA+GGG 0.501269 2_1:-65899265 None:intergenic
GCCCGCTCGACTGATGCTCT+TGG 0.504243 2_1:-65902373 None:intergenic
CCTATGTGTTGATAAATACA+AGG 0.512785 2_1:-65902697 None:intergenic
GCATCAGAATCTGCATAAGC+AGG 0.514844 2_1:-65901481 None:intergenic
ATCCAAAGCATTGACACATC+CGG 0.515020 2_1:-65902038 None:intergenic
GAAGAACCTGACAGTGGTGC+TGG 0.515078 2_1:+65900631 Msa0218630:CDS
AAGTGCATACCTTGTGAAGA+TGG 0.517267 2_1:-65899812 None:intergenic
GGAGAAATTGTACAAAGAAA+CGG 0.517355 2_1:+65902115 Msa0218630:CDS
TCCTGAAACATTCTTGATTG+TGG 0.519192 2_1:+65900670 Msa0218630:CDS
TGGTGCTGGAAGAATTTCCT+GGG 0.521506 2_1:+65900645 Msa0218630:CDS
ATCAAGTGATGAAGGAGAAA+AGG 0.523343 2_1:-65901572 None:intergenic
CATTTGAGAACAGCATTAGC+CGG 0.524224 2_1:+65902019 Msa0218630:CDS
CCCTGCCACCGACCAAGACC+TGG 0.524414 2_1:-65901205 None:intergenic
CTAGTGCTGCCGTTGATCAG+AGG 0.525231 2_1:+65901278 Msa0218630:CDS
ACTGCTCGAGGTGGTACGGC+TGG 0.530026 2_1:+65902094 Msa0218630:CDS
AGCTCTTGATAGAATAGGCT+CGG 0.531807 2_1:-65900879 None:intergenic
TTCCAAAGAATGCTTCCACC+AGG 0.532570 2_1:-65901010 None:intergenic
ACCAAGAGCATCAGTCGAGC+GGG 0.538088 2_1:+65902372 Msa0218630:CDS
TTTCCACATCTTGAAACAAG+AGG 0.542911 2_1:-65900569 None:intergenic
CCTTGTATTTATCAACACAT+AGG 0.544116 2_1:+65902697 Msa0218630:three_prime_UTR
GCGCGAAAATCAGGGTTTGA+AGG 0.547646 2_1:+65902153 Msa0218630:CDS
GACTTGAAGAACAACTCTGA+GGG 0.551619 2_1:-65901978 None:intergenic
GGTGGAAGCATTCTTTGGAA+TGG 0.551948 2_1:+65901013 Msa0218630:CDS
TAGTGCTGCCGTTGATCAGA+GGG 0.552150 2_1:+65901279 Msa0218630:CDS
TCTATCTATGATGGCTTGCA+CGG 0.554280 2_1:+65900937 Msa0218630:CDS
AAACTTCTTCCTCAAAAGAA+GGG 0.557294 2_1:+65901322 Msa0218630:CDS
GCTGTTGTGCGCGAAAATCA+GGG 0.560005 2_1:+65902145 Msa0218630:CDS
GGATGGCCGATGATGATGTA+TGG 0.560892 2_1:+65900974 Msa0218630:CDS
AACTAATTACTCTATCATCA+TGG 0.564175 2_1:-65899349 None:intergenic
ATCGGCCATCCAAATAAACT+CGG 0.566431 2_1:-65900962 None:intergenic
ACTTGCCAGGTCTTGGTCGG+TGG 0.567054 2_1:+65901200 Msa0218630:CDS
AAACCACCACTCGGATTCTG+AGG 0.569410 2_1:-65901062 None:intergenic
ATGATCTCGATGACTCAGGA+AGG 0.570271 2_1:-65901717 None:intergenic
ACAGTGCACGTGGTTCTCCT+CGG 0.570458 2_1:+65902266 Msa0218630:CDS
TGCAATGATCTCGATGACTC+AGG 0.571453 2_1:-65901721 None:intergenic
CTCGACTGATGCTCTTGGTG+TGG 0.573680 2_1:-65902368 None:intergenic
ATGGTATAAATACCGGCGCT+AGG 0.573781 2_1:+65901032 Msa0218630:CDS
CGAAAATCAGGGTTTGAAGG+TGG 0.575204 2_1:+65902156 Msa0218630:CDS
CCTCGAGCAGTCGATCGAGC+AGG 0.575521 2_1:-65902082 None:intergenic
ATCGACTGCTCGAGGTGGTA+CGG 0.579037 2_1:+65902090 Msa0218630:CDS
ATGACGGACGAATACGAAGA+AGG 0.579734 2_1:+65900773 Msa0218630:CDS
GAAGAAGTCCGAGTAAGAAA+CGG 0.583295 2_1:-65901357 None:intergenic
TCCAAAGAATGCTTCCACCA+GGG 0.589108 2_1:-65901009 None:intergenic
CGGTGGCAGGGTGGACAAGG+TGG 0.592330 2_1:+65901217 Msa0218630:CDS
TGTGGATACAGAACGATCAA+GGG 0.592917 2_1:-65902350 None:intergenic
TTCTCGTTATCAGAGAGCCA+AGG 0.599221 2_1:-65901875 None:intergenic
CAAAAGAACCCTCTGATCAA+CGG 0.599347 2_1:-65901287 None:intergenic
AGCACTTGCCAGGTCTTGGT+CGG 0.602120 2_1:+65901197 Msa0218630:CDS
CACCAAGAGCATCAGTCGAG+CGG 0.602703 2_1:+65902371 Msa0218630:CDS
TGAAATATATGTCTAGTGCT+CGG 0.602823 2_1:-65901756 None:intergenic
GCTTCAAAATGGCTCCACCT+GGG 0.603801 2_1:+65899477 Msa0218630:exon
GAGATGCATGAGCACATGCA+TGG 0.604027 2_1:-65901940 None:intergenic
AATTGTACAAAGAAACGGAT+GGG 0.604670 2_1:+65902120 Msa0218630:CDS
TGCAGAATACCTTGAAATGA+CGG 0.605457 2_1:+65900757 Msa0218630:CDS
CCTGCTCGATCGACTGCTCG+AGG 0.610208 2_1:+65902082 Msa0218630:CDS
TGCACTGTGAGATTTGGTCA+CGG 0.616859 2_1:-65902251 None:intergenic
ATTAAAACGATGCGAGCGAG+AGG 0.617106 2_1:+65901127 Msa0218630:CDS
AAACTTCCATACATCATCAT+CGG 0.618818 2_1:-65900980 None:intergenic
GCTTGCGGATCAAGTGATGA+AGG 0.619541 2_1:-65901580 None:intergenic
GATAGAATGAGTACTAGAGT+CGG 0.620497 2_1:+65902184 Msa0218630:CDS
GGTCTTGGTCGGTGGCAGGG+TGG 0.626378 2_1:+65901208 Msa0218630:CDS
GGATGTGTCAATGCTTTGGA+TGG 0.630355 2_1:+65902040 Msa0218630:CDS
GGACCTCAGAATCCGAGTGG+TGG 0.632921 2_1:+65901059 Msa0218630:CDS
ACCAAATCTCACAGTGCACG+TGG 0.635145 2_1:+65902256 Msa0218630:CDS
TGATCTCGATGACTCAGGAA+GGG 0.637384 2_1:-65901716 None:intergenic
GGTGCTGGAAGAATTTCCTG+GGG 0.637441 2_1:+65900646 Msa0218630:CDS
TGAGGTCCGAATCCTAGCGC+CGG 0.638429 2_1:-65901044 None:intergenic
GATGTCTTCAAACCACCACT+CGG 0.642289 2_1:-65901071 None:intergenic
AATACATTATGGCACCTGCA+AGG 0.648443 2_1:-65901165 None:intergenic
GGTTACTTGCAAGAAAACTC+GGG 0.650025 2_1:+65902287 Msa0218630:CDS
TCAGACGACGATGATGCTTG+CGG 0.656201 2_1:-65901595 None:intergenic
ATTGACACAGTAATATCACT+TGG 0.657084 2_1:-65899774 None:intergenic
TCGGCCATCCAAATAAACTC+GGG 0.657524 2_1:-65900961 None:intergenic
AAATTGTACAAAGAAACGGA+TGG 0.658614 2_1:+65902119 Msa0218630:CDS
AGACTTGAAGAACAACTCTG+AGG 0.663274 2_1:-65901979 None:intergenic
CTATGTGTTGATAAATACAA+GGG 0.664074 2_1:-65902696 None:intergenic
TGTCAATTTCCATCTTCACA+AGG 0.664191 2_1:+65899803 Msa0218630:CDS
TGTGTCTGGACTTATGGGAA+CGG 0.677659 2_1:-65902399 None:intergenic
TCATCGAGATCATTGCATGA+CGG 0.684043 2_1:+65901727 Msa0218630:CDS
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 2_1:-65899491 None:intergenic
GTGGATACAGAACGATCAAG+GGG 0.686942 2_1:-65902349 None:intergenic
GCTCGATCGACTGCTCGAGG+TGG 0.696171 2_1:+65902085 Msa0218630:CDS
ACTTGAAGAACAACTCTGAG+GGG 0.701967 2_1:-65901977 None:intergenic
GTGCTGGAAGAATTTCCTGG+GGG 0.715345 2_1:+65900647 Msa0218630:CDS
TTCGGACCTCAGAATCCGAG+TGG 0.719040 2_1:+65901056 Msa0218630:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 2_1:-65900748 None:intergenic
ATACACATGAAAGTGTTGTG+TGG 0.736317 2_1:+65902483 Msa0218630:three_prime_UTR
CTATGAGAAGAACCTGACAG+TGG 0.738065 2_1:+65900625 Msa0218630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAGATTAAAAAAGTAAA+AGG + chr2_1:65899436-65899455 Msa0218630:five_prime_UTR 10.0%
!! TTGTTACATGAATTATTTAT+TGG + chr2_1:65899574-65899593 Msa0218630:intron 15.0%
!!! AAAATGACATTTTTTTTTAG+TGG + chr2_1:65899642-65899661 Msa0218630:intron 15.0%
!!! TTTTTTTTTTCCTGTTTTTT+TGG + chr2_1:65899716-65899735 Msa0218630:intron 15.0%
!!! AAAACTTTTTTACATCAATA+GGG - chr2_1:65900037-65900056 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG - chr2_1:65900038-65900057 None:intergenic 15.0%
!! AGAATTCAGTAAAATAAAAA+AGG + chr2_1:65902219-65902238 Msa0218630:CDS 15.0%
!! ATGAATAAATGAAAATAAAG+AGG - chr2_1:65902461-65902480 None:intergenic 15.0%
!!! GAAAAAAAAGTATGAATGAA+GGG - chr2_1:65899278-65899297 None:intergenic 20.0%
!!! AGAAAAAAAAGTATGAATGA+AGG - chr2_1:65899279-65899298 None:intergenic 20.0%
!! TGATAGAGTAATTAGTTATT+CGG + chr2_1:65899355-65899374 Msa0218630:five_prime_UTR 20.0%
!! TAGATTAAAAAAGTAAAAGG+AGG + chr2_1:65899439-65899458 Msa0218630:five_prime_UTR 20.0%
!! AGATTAAAAAAGTAAAAGGA+GGG + chr2_1:65899440-65899459 Msa0218630:five_prime_UTR 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG - chr2_1:65899729-65899748 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG + chr2_1:65899729-65899748 Msa0218630:intron 20.0%
!! ACAAAAGAACAAAAATGAAT+AGG - chr2_1:65899940-65899959 None:intergenic 20.0%
!! AAACAAACTAACAGTAAAAA+AGG - chr2_1:65900006-65900025 None:intergenic 20.0%
!!! ACTAAATGCATTTAAAATGT+TGG - chr2_1:65900282-65900301 None:intergenic 20.0%
!!! AGATGAATATTTTGAGATAT+TGG + chr2_1:65900351-65900370 Msa0218630:intron 20.0%
!! GATATTGGAGAAAATTTATT+TGG + chr2_1:65900366-65900385 Msa0218630:intron 20.0%
!! ATGAAAATAAAGAGGAAATA+TGG - chr2_1:65902453-65902472 None:intergenic 20.0%
!!! GTTTAAATTTTTTTGTACGT+GGG + chr2_1:65902505-65902524 Msa0218630:three_prime_UTR 20.0%
!! GCTTAAATAAAAATGCATAA+TGG - chr2_1:65902560-65902579 None:intergenic 20.0%
!!! TTTCTTTGTACATTTTTCAT+TGG + chr2_1:65902632-65902651 Msa0218630:three_prime_UTR 20.0%
! AACTAATTACTCTATCATCA+TGG - chr2_1:65899352-65899371 None:intergenic 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG + chr2_1:65899733-65899752 Msa0218630:intron 25.0%
! TAGATTATTAGTGGAAGAAA+AGG - chr2_1:65899857-65899876 None:intergenic 25.0%
! TACGATGAATAGATTATTAG+TGG - chr2_1:65899866-65899885 None:intergenic 25.0%
! TATTCATCGTAATTTGATAG+TGG + chr2_1:65899875-65899894 Msa0218630:intron 25.0%
!!! TTGTTGGGATTTTTTTTTTG+TGG + chr2_1:65899903-65899922 Msa0218630:intron 25.0%
! TATAAAGTCACACATATGAT+TGG - chr2_1:65900080-65900099 None:intergenic 25.0%
! GTTATGTGACTACAATTAAA+AGG + chr2_1:65900325-65900344 Msa0218630:intron 25.0%
! ATTGGAGAAAATTTATTTGG+AGG + chr2_1:65900369-65900388 Msa0218630:intron 25.0%
! CACGTAATTTATCATATCAA+AGG - chr2_1:65900501-65900520 None:intergenic 25.0%
!!! AGTTGCGCAATATATTTTTA+TGG - chr2_1:65900829-65900848 None:intergenic 25.0%
! ATAAAAATATATTGCGCAAC+TGG + chr2_1:65900828-65900847 Msa0218630:CDS 25.0%
! CTTCCTAGAATAATACATTA+TGG - chr2_1:65901179-65901198 None:intergenic 25.0%
! ATTCCATAAGTACAAAATGA+TGG - chr2_1:65901540-65901559 None:intergenic 25.0%
!!! AAGTTGATAGATAGCTATAT+TGG + chr2_1:65901637-65901656 Msa0218630:CDS 25.0%
!!! GGTTTAAATTTTTTTGTACG+TGG + chr2_1:65902504-65902523 Msa0218630:three_prime_UTR 25.0%
! AAAAAACCAACTTTACATGA+GGG - chr2_1:65902615-65902634 None:intergenic 25.0%
!! CTATGTGTTGATAAATACAA+GGG - chr2_1:65902699-65902718 None:intergenic 25.0%
!! ATTTTAGACATCAATTGTGA+AGG + chr2_1:65902760-65902779 Msa0218630:three_prime_UTR 25.0%
! GTGATCAAAAGAAAGAATAT+TGG + chr2_1:65902791-65902810 Msa0218630:three_prime_UTR 25.0%
GTCGAAAAATAAGTAAAGTC+TGG + chr2_1:65899377-65899396 Msa0218630:five_prime_UTR 30.0%
TTGTTTCCTTTGCTTCAAAA+TGG + chr2_1:65899466-65899485 Msa0218630:exon 30.0%
ATAAAGTTGCATGTATGACT+TGG - chr2_1:65899671-65899690 None:intergenic 30.0%
ATTGACACAGTAATATCACT+TGG - chr2_1:65899777-65899796 None:intergenic 30.0%
GATATTACTGTGTCAATTGA+TGG + chr2_1:65899780-65899799 Msa0218630:CDS 30.0%
! AAAGTAGGAGCACATTAATT+GGG - chr2_1:65899979-65899998 None:intergenic 30.0%
AGTAAAAAAGGTCACAAAGT+AGG - chr2_1:65899994-65900013 None:intergenic 30.0%
TTGTGATCTCTAGTTAATGA+GGG - chr2_1:65900193-65900212 None:intergenic 30.0%
ATTGTGATCTCTAGTTAATG+AGG - chr2_1:65900194-65900213 None:intergenic 30.0%
ATATTTGCACGGTCAAATAA+GGG + chr2_1:65900427-65900446 Msa0218630:intron 30.0%
AATTACGTGAAATGTTGTCT+TGG + chr2_1:65900512-65900531 Msa0218630:intron 30.0%
! CAACTGGAAAGATTGTATTT+TGG + chr2_1:65900844-65900863 Msa0218630:CDS 30.0%
! TTTTCAGCTCTTGATAGAAT+AGG - chr2_1:65900887-65900906 None:intergenic 30.0%
AGCTGAAAATCTTCATTTAG+TGG + chr2_1:65900898-65900917 Msa0218630:CDS 30.0%
TTTAAACGCTCTATCTATGA+TGG + chr2_1:65900928-65900947 Msa0218630:CDS 30.0%
AAACTTCCATACATCATCAT+CGG - chr2_1:65900983-65901002 None:intergenic 30.0%
!! CTTTGGAATGGTATAAATAC+CGG + chr2_1:65901025-65901044 Msa0218630:CDS 30.0%
GTGCCATAATGTATTATTCT+AGG + chr2_1:65901173-65901192 Msa0218630:CDS 30.0%
AAACTTCTTCCTCAAAAGAA+GGG + chr2_1:65901322-65901341 Msa0218630:CDS 30.0%
! AAAGATTTTCCCTTCTTTTG+AGG - chr2_1:65901334-65901353 None:intergenic 30.0%
! AATCTTTGGAGAGAAGAATA+GGG + chr2_1:65901419-65901438 Msa0218630:CDS 30.0%
! TGTCCATCATTTTGTACTTA+TGG + chr2_1:65901534-65901553 Msa0218630:CDS 30.0%
TGAAATATATGTCTAGTGCT+CGG - chr2_1:65901759-65901778 None:intergenic 30.0%
AGCACTAGACATATATTTCA+AGG + chr2_1:65901759-65901778 Msa0218630:CDS 30.0%
! GTTAGTTTGATGAATGCTTA+CGG + chr2_1:65901836-65901855 Msa0218630:intron 30.0%
GGAGAAATTGTACAAAGAAA+CGG + chr2_1:65902115-65902134 Msa0218630:CDS 30.0%
AAATTGTACAAAGAAACGGA+TGG + chr2_1:65902119-65902138 Msa0218630:CDS 30.0%
AATTGTACAAAGAAACGGAT+GGG + chr2_1:65902120-65902139 Msa0218630:CDS 30.0%
!!! AATTTTTTTGTACGTGGGAA+AGG + chr2_1:65902510-65902529 Msa0218630:three_prime_UTR 30.0%
!!! ATTTTTTTGTACGTGGGAAA+GGG + chr2_1:65902511-65902530 Msa0218630:three_prime_UTR 30.0%
GAAAAAACCAACTTTACATG+AGG - chr2_1:65902616-65902635 None:intergenic 30.0%
!!! ATTGTCTTGTTTTGTTTGTG+TGG + chr2_1:65902666-65902685 Msa0218630:three_prime_UTR 30.0%
!!! TTGTCTTGTTTTGTTTGTGT+GGG + chr2_1:65902667-65902686 Msa0218630:three_prime_UTR 30.0%
! CCTATGTGTTGATAAATACA+AGG - chr2_1:65902700-65902719 None:intergenic 30.0%
CCTTGTATTTATCAACACAT+AGG + chr2_1:65902697-65902716 Msa0218630:three_prime_UTR 30.0%
!! CATTCTTCATTTTGACATAC+TGG + chr2_1:65902722-65902741 Msa0218630:three_prime_UTR 30.0%
TATGAATGAAGGGAAGAGAA+GGG - chr2_1:65899268-65899287 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG + chr2_1:65899747-65899766 Msa0218630:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG + chr2_1:65899748-65899767 Msa0218630:intron 35.0%
TGTCAATTTCCATCTTCACA+AGG + chr2_1:65899803-65899822 Msa0218630:CDS 35.0%
!! TTTGATAGTGGAGTAGTTGT+TGG + chr2_1:65899887-65899906 Msa0218630:intron 35.0%
!! TTGATAGTGGAGTAGTTGTT+GGG + chr2_1:65899888-65899907 Msa0218630:intron 35.0%
! CAAAGTAGGAGCACATTAAT+TGG - chr2_1:65899980-65899999 None:intergenic 35.0%
TGTGATCTCTAGTTAATGAG+GGG - chr2_1:65900192-65900211 None:intergenic 35.0%
! GTTCACTTGATCTACTGTTT+TGG + chr2_1:65900300-65900319 Msa0218630:intron 35.0%
GAGAAAATTTATTTGGAGGC+TGG + chr2_1:65900373-65900392 Msa0218630:intron 35.0%
TATTTGACCGTGCAAATATG+CGG - chr2_1:65900426-65900445 None:intergenic 35.0%
CATATTTGCACGGTCAAATA+AGG + chr2_1:65900426-65900445 Msa0218630:intron 35.0%
TATTTGCACGGTCAAATAAG+GGG + chr2_1:65900428-65900447 Msa0218630:intron 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG + chr2_1:65900566-65900585 Msa0218630:CDS 35.0%
TTTCCACATCTTGAAACAAG+AGG - chr2_1:65900572-65900591 None:intergenic 35.0%
TCTTGGAATCTTCATAGGTA+CGG - chr2_1:65900601-65900620 None:intergenic 35.0%
GGTATTCTTGGAATCTTCAT+AGG - chr2_1:65900606-65900625 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG - chr2_1:65900618-65900637 None:intergenic 35.0%
TCCTGAAACATTCTTGATTG+TGG + chr2_1:65900670-65900689 Msa0218630:CDS 35.0%
ATTGTGGCCAAATTCTGTTA+CGG + chr2_1:65900686-65900705 Msa0218630:CDS 35.0%
TGCAGAATACCTTGAAATGA+CGG + chr2_1:65900757-65900776 Msa0218630:CDS 35.0%
GAAACTTCTTCCTCAAAAGA+AGG + chr2_1:65901321-65901340 Msa0218630:CDS 35.0%
!! TCTTTTTGCCGTTTCTTACT+CGG + chr2_1:65901349-65901368 Msa0218630:CDS 35.0%
! GAATCTTTGGAGAGAAGAAT+AGG + chr2_1:65901418-65901437 Msa0218630:CDS 35.0%
ATCAAGTGATGAAGGAGAAA+AGG - chr2_1:65901575-65901594 None:intergenic 35.0%
!! ATGCATGGATTGAGAGTTTT+TGG - chr2_1:65901928-65901947 None:intergenic 35.0%
CTCATGCATCTCAAAATGAT+AGG + chr2_1:65901951-65901970 Msa0218630:CDS 35.0%
GATAGAATGAGTACTAGAGT+CGG + chr2_1:65902184-65902203 Msa0218630:CDS 35.0%
AGAAAAACTTTCTGTGTGTC+TGG - chr2_1:65902416-65902435 None:intergenic 35.0%
!! ATACACATGAAAGTGTTGTG+TGG + chr2_1:65902483-65902502 Msa0218630:three_prime_UTR 35.0%
CTTTACATGAGGGAAAGAAA+TGG - chr2_1:65902605-65902624 None:intergenic 35.0%
TTCTTTCCCTCATGTAAAGT+TGG + chr2_1:65902606-65902625 Msa0218630:three_prime_UTR 35.0%
!!! TGTCTTGTTTTGTTTGTGTG+GGG + chr2_1:65902668-65902687 Msa0218630:three_prime_UTR 35.0%
GTATGAATGAAGGGAAGAGA+AGG - chr2_1:65899269-65899288 None:intergenic 40.0%
CTTTCTGGGTTTCATCTTCA+AGG + chr2_1:65899503-65899522 Msa0218630:CDS 40.0%
TTCATCTTCAAGGTCATCAC+TGG + chr2_1:65899513-65899532 Msa0218630:CDS 40.0%
AAGTGCATACCTTGTGAAGA+TGG - chr2_1:65899815-65899834 None:intergenic 40.0%
TGAATTCCCGCATATTTGCA+CGG + chr2_1:65900416-65900435 Msa0218630:intron 40.0%
ATTTGACCGTGCAAATATGC+GGG - chr2_1:65900425-65900444 None:intergenic 40.0%
AAGAGGAAACTGCAGTTTCA+CGG - chr2_1:65900555-65900574 None:intergenic 40.0%
CTTGGAATCTTCATAGGTAC+GGG - chr2_1:65900600-65900619 None:intergenic 40.0%
GCCACAATCAAGAATGTTTC+AGG - chr2_1:65900674-65900693 None:intergenic 40.0%
TTGTGGCCAAATTCTGTTAC+GGG + chr2_1:65900687-65900706 Msa0218630:CDS 40.0%
ACGAAACCCGTAACAGAATT+TGG - chr2_1:65900696-65900715 None:intergenic 40.0%
AGCTCTTGATAGAATAGGCT+CGG - chr2_1:65900882-65900901 None:intergenic 40.0%
! TCTATCTATGATGGCTTGCA+CGG + chr2_1:65900937-65900956 Msa0218630:CDS 40.0%
ATCGGCCATCCAAATAAACT+CGG - chr2_1:65900965-65900984 None:intergenic 40.0%
! TATGGAAGTTTTCAAAGCCC+TGG + chr2_1:65900992-65901011 Msa0218630:CDS 40.0%
!! TCGCTCGCATCGTTTTAATA+AGG - chr2_1:65901126-65901145 None:intergenic 40.0%
AATACATTATGGCACCTGCA+AGG - chr2_1:65901168-65901187 None:intergenic 40.0%
CAAAAGAACCCTCTGATCAA+CGG - chr2_1:65901290-65901309 None:intergenic 40.0%
GAAGAAGTCCGAGTAAGAAA+CGG - chr2_1:65901360-65901379 None:intergenic 40.0%
TTCTTCTCTCCAAAGATTCC+TGG - chr2_1:65901417-65901436 None:intergenic 40.0%
!! GCAAGAGCACATAACTTTTC+TGG - chr2_1:65901691-65901710 None:intergenic 40.0%
TCATCGAGATCATTGCATGA+CGG + chr2_1:65901727-65901746 Msa0218630:CDS 40.0%
ACTTGAAGAACAACTCTGAG+GGG - chr2_1:65901980-65901999 None:intergenic 40.0%
GACTTGAAGAACAACTCTGA+GGG - chr2_1:65901981-65902000 None:intergenic 40.0%
AGACTTGAAGAACAACTCTG+AGG - chr2_1:65901982-65902001 None:intergenic 40.0%
CATTTGAGAACAGCATTAGC+CGG + chr2_1:65902019-65902038 Msa0218630:CDS 40.0%
!! ATCCAAAGCATTGACACATC+CGG - chr2_1:65902041-65902060 None:intergenic 40.0%
! AGTTTTCTTGCAAGTAACCG+AGG - chr2_1:65902286-65902305 None:intergenic 40.0%
CGGTTACTTGCAAGAAAACT+CGG + chr2_1:65902286-65902305 Msa0218630:CDS 40.0%
GGTTACTTGCAAGAAAACTC+GGG + chr2_1:65902287-65902306 Msa0218630:CDS 40.0%
GGTTGAGCATCTGAAAATCT+AGG - chr2_1:65902325-65902344 None:intergenic 40.0%
TGTGGATACAGAACGATCAA+GGG - chr2_1:65902353-65902372 None:intergenic 40.0%
TTCTGTGTGTCTGGACTTAT+GGG - chr2_1:65902407-65902426 None:intergenic 40.0%
TTTCTGTGTGTCTGGACTTA+TGG - chr2_1:65902408-65902427 None:intergenic 40.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG - chr2_1:65899475-65899494 None:intergenic 45.0%
CACTGTCAGGTTCTTCTCAT+AGG - chr2_1:65900627-65900646 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG + chr2_1:65900625-65900644 Msa0218630:CDS 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG + chr2_1:65900645-65900664 Msa0218630:CDS 45.0%
CGTATTCGTCCGTCATTTCA+AGG - chr2_1:65900769-65900788 None:intergenic 45.0%
!! ATGACGGACGAATACGAAGA+AGG + chr2_1:65900773-65900792 Msa0218630:CDS 45.0%
ATAGAATAGGCTCGGAGCTT+TGG - chr2_1:65900874-65900893 None:intergenic 45.0%
TCGGCCATCCAAATAAACTC+GGG - chr2_1:65900964-65900983 None:intergenic 45.0%
TCCAAAGAATGCTTCCACCA+GGG - chr2_1:65901012-65901031 None:intergenic 45.0%
TTCCAAAGAATGCTTCCACC+AGG - chr2_1:65901013-65901032 None:intergenic 45.0%
!! GGTGGAAGCATTCTTTGGAA+TGG + chr2_1:65901013-65901032 Msa0218630:CDS 45.0%
ATGGTATAAATACCGGCGCT+AGG + chr2_1:65901032-65901051 Msa0218630:CDS 45.0%
TAAATACCGGCGCTAGGATT+CGG + chr2_1:65901038-65901057 Msa0218630:CDS 45.0%
GATGTCTTCAAACCACCACT+CGG - chr2_1:65901074-65901093 None:intergenic 45.0%
ATTAAAACGATGCGAGCGAG+AGG + chr2_1:65901127-65901146 Msa0218630:CDS 45.0%
!! TATTCTAGGAAGCACTTGCC+AGG + chr2_1:65901187-65901206 Msa0218630:CDS 45.0%
! CGTTGATCAGAGGGTTCTTT+TGG + chr2_1:65901288-65901307 Msa0218630:CDS 45.0%
GAATGTTGATCAAGCTTGCC+AGG + chr2_1:65901396-65901415 Msa0218630:CDS 45.0%
TCAAGCTTGCCAGGAATCTT+TGG + chr2_1:65901405-65901424 Msa0218630:CDS 45.0%
GAGAAGAATAGGGATGCAAC+TGG + chr2_1:65901429-65901448 Msa0218630:CDS 45.0%
GCATCAGAATCTGCATAAGC+AGG - chr2_1:65901484-65901503 None:intergenic 45.0%
CATCGTCGTCTGAATCGTTA+AGG + chr2_1:65901605-65901624 Msa0218630:CDS 45.0%
TGATCTCGATGACTCAGGAA+GGG - chr2_1:65901719-65901738 None:intergenic 45.0%
ATGATCTCGATGACTCAGGA+AGG - chr2_1:65901720-65901739 None:intergenic 45.0%
TGCAATGATCTCGATGACTC+AGG - chr2_1:65901724-65901743 None:intergenic 45.0%
ATCGATCGAGAATGTAGCTC+AGG - chr2_1:65901794-65901813 None:intergenic 45.0%
TTCTCGTTATCAGAGAGCCA+AGG - chr2_1:65901878-65901897 None:intergenic 45.0%
! GGATGTGTCAATGCTTTGGA+TGG + chr2_1:65902040-65902059 Msa0218630:CDS 45.0%
CGAAAATCAGGGTTTGAAGG+TGG + chr2_1:65902156-65902175 Msa0218630:CDS 45.0%
TCAGGGTTTGAAGGTGGATA+TGG + chr2_1:65902162-65902181 Msa0218630:CDS 45.0%
! TGCACTGTGAGATTTGGTCA+CGG - chr2_1:65902254-65902273 None:intergenic 45.0%
GTGGATACAGAACGATCAAG+GGG - chr2_1:65902352-65902371 None:intergenic 45.0%
GTGTGGATACAGAACGATCA+AGG - chr2_1:65902354-65902373 None:intergenic 45.0%
TGTGTCTGGACTTATGGGAA+CGG - chr2_1:65902402-65902421 None:intergenic 45.0%
! TTTTGTACGTGGGAAAGGGA+AGG + chr2_1:65902515-65902534 Msa0218630:three_prime_UTR 45.0%
!!! TTTTTTGAATAAAAAAAAAA+GGG - chr2_1:65899306-65899325 None:intergenic 5.0%
!!! TTTTTTTGAATAAAAAAAAA+AGG - chr2_1:65899307-65899326 None:intergenic 5.0%
GGGAAGAGAAGGGAGAAGAA+AGG - chr2_1:65899258-65899277 None:intergenic 50.0%
TGCTTCAAAATGGCTCCACC+TGG + chr2_1:65899476-65899495 Msa0218630:exon 50.0%
GCTTCAAAATGGCTCCACCT+GGG + chr2_1:65899477-65899496 Msa0218630:exon 50.0%
TGAAACCCAGAAAGCTTCCC+AGG - chr2_1:65899497-65899516 None:intergenic 50.0%
ATTCTTCCAGCACCACTGTC+AGG - chr2_1:65900640-65900659 None:intergenic 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG + chr2_1:65900644-65900663 Msa0218630:CDS 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG + chr2_1:65900646-65900665 Msa0218630:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG + chr2_1:65900647-65900666 Msa0218630:CDS 50.0%
AAGAATGTTTCAGGACCCCC+AGG - chr2_1:65900665-65900684 None:intergenic 50.0%
! CAAGGTATTCTGCAGCACAG+TGG - chr2_1:65900751-65900770 None:intergenic 50.0%
TGCACGGACCCGAGTTTATT+TGG + chr2_1:65900953-65900972 Msa0218630:CDS 50.0%
CGGACCCGAGTTTATTTGGA+TGG + chr2_1:65900957-65900976 Msa0218630:CDS 50.0%
! GGATGGCCGATGATGATGTA+TGG + chr2_1:65900974-65900993 Msa0218630:CDS 50.0%
! GGAAGTTTTCAAAGCCCTGG+TGG + chr2_1:65900995-65901014 Msa0218630:CDS 50.0%
AAACCACCACTCGGATTCTG+AGG - chr2_1:65901065-65901084 None:intergenic 50.0%
TATGGCACCTGCAAGGCTTT+CGG - chr2_1:65901161-65901180 None:intergenic 50.0%
TAGTGCTGCCGTTGATCAGA+GGG + chr2_1:65901279-65901298 Msa0218630:CDS 50.0%
GCTTGCGGATCAAGTGATGA+AGG - chr2_1:65901583-65901602 None:intergenic 50.0%
TCAGACGACGATGATGCTTG+CGG - chr2_1:65901598-65901617 None:intergenic 50.0%
GAATGCTTACGGCACTTTGC+AGG + chr2_1:65901847-65901866 Msa0218630:intron 50.0%
GAGATGCATGAGCACATGCA+TGG - chr2_1:65901943-65901962 None:intergenic 50.0%
AGCCGGATGTGTCAATGCTT+TGG + chr2_1:65902036-65902055 Msa0218630:CDS 50.0%
TGCTGTTGTGCGCGAAAATC+AGG + chr2_1:65902144-65902163 Msa0218630:CDS 50.0%
GCTGTTGTGCGCGAAAATCA+GGG + chr2_1:65902145-65902164 Msa0218630:CDS 50.0%
GCGCGAAAATCAGGGTTTGA+AGG + chr2_1:65902153-65902172 Msa0218630:CDS 50.0%
ACCAAATCTCACAGTGCACG+TGG + chr2_1:65902256-65902275 Msa0218630:CDS 50.0%
ACCACGTGCACTGTGAGATT+TGG - chr2_1:65902260-65902279 None:intergenic 50.0%
ACAGAACGATCAAGGGGTTC+AGG - chr2_1:65902346-65902365 None:intergenic 50.0%
CTCCACCTGGGAAGCTTTCT+GGG + chr2_1:65899489-65899508 Msa0218630:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG - chr2_1:65899494-65899513 None:intergenic 55.0%
GAAGAACCTGACAGTGGTGC+TGG + chr2_1:65900631-65900650 Msa0218630:CDS 55.0%
!! GCCCTGGTGGAAGCATTCTT+TGG + chr2_1:65901008-65901027 Msa0218630:CDS 55.0%
TTCGGACCTCAGAATCCGAG+TGG + chr2_1:65901056-65901075 Msa0218630:CDS 55.0%
ATGAGACCCGAAAGCCTTGC+AGG + chr2_1:65901151-65901170 Msa0218630:CDS 55.0%
ATGGCACCTGCAAGGCTTTC+GGG - chr2_1:65901160-65901179 None:intergenic 55.0%
!! AGGAAGCACTTGCCAGGTCT+TGG + chr2_1:65901193-65901212 Msa0218630:CDS 55.0%
AGCACTTGCCAGGTCTTGGT+CGG + chr2_1:65901197-65901216 Msa0218630:CDS 55.0%
TGATCAACGGCAGCACTAGC+AGG - chr2_1:65901277-65901296 None:intergenic 55.0%
CTAGTGCTGCCGTTGATCAG+AGG + chr2_1:65901278-65901297 Msa0218630:CDS 55.0%
GCACTTTGCAGGCTCATCCT+TGG + chr2_1:65901858-65901877 Msa0218630:intron 55.0%
ATCGACTGCTCGAGGTGGTA+CGG + chr2_1:65902090-65902109 Msa0218630:CDS 55.0%
ACAGTGCACGTGGTTCTCCT+CGG + chr2_1:65902266-65902285 Msa0218630:CDS 55.0%
CTCGACTGATGCTCTTGGTG+TGG - chr2_1:65902371-65902390 None:intergenic 55.0%
!! CACCAAGAGCATCAGTCGAG+CGG + chr2_1:65902371-65902390 Msa0218630:CDS 55.0%
!! ACCAAGAGCATCAGTCGAGC+GGG + chr2_1:65902372-65902391 Msa0218630:CDS 55.0%
GCTCCACCTGGGAAGCTTTC+TGG + chr2_1:65899488-65899507 Msa0218630:CDS 60.0%
TGAGGTCCGAATCCTAGCGC+CGG - chr2_1:65901047-65901066 None:intergenic 60.0%
GGACCTCAGAATCCGAGTGG+TGG + chr2_1:65901059-65901078 Msa0218630:CDS 60.0%
ACTTGCCAGGTCTTGGTCGG+TGG + chr2_1:65901200-65901219 Msa0218630:CDS 60.0%
GTCGATCGAGCAGGAACTGC+TGG - chr2_1:65902076-65902095 None:intergenic 60.0%
CCAGGTCTTGGTCGGTGGCA+GGG + chr2_1:65901205-65901224 Msa0218630:CDS 65.0%
CCTCGAGCAGTCGATCGAGC+AGG - chr2_1:65902085-65902104 None:intergenic 65.0%
CCTGCTCGATCGACTGCTCG+AGG + chr2_1:65902082-65902101 Msa0218630:CDS 65.0%
GCTCGATCGACTGCTCGAGG+TGG + chr2_1:65902085-65902104 Msa0218630:CDS 65.0%
ACTGCTCGAGGTGGTACGGC+TGG + chr2_1:65902094-65902113 Msa0218630:CDS 65.0%
GCCCGCTCGACTGATGCTCT+TGG - chr2_1:65902376-65902395 None:intergenic 65.0%
GCCAGGTCTTGGTCGGTGGC+AGG + chr2_1:65901204-65901223 Msa0218630:CDS 70.0%
CCCTGCCACCGACCAAGACC+TGG - chr2_1:65901208-65901227 None:intergenic 70.0%
GGTCTTGGTCGGTGGCAGGG+TGG + chr2_1:65901208-65901227 Msa0218630:CDS 70.0%
GGTCGGTGGCAGGGTGGACA+AGG + chr2_1:65901214-65901233 Msa0218630:CDS 70.0%
CGGTGGCAGGGTGGACAAGG+TGG + chr2_1:65901217-65901236 Msa0218630:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 65899226 65902827 65899226 ID=Msa0218630;Name=Msa0218630
chr2_1 mRNA 65899226 65902827 65899226 ID=Msa0218630-mRNA-1;Parent=Msa0218630;Name=Msa0218630-mRNA-1;_AED=0.05;_eAED=0.05;_QI=259|1|1|1|1|1|4|389|634
chr2_1 exon 65899226 65899534 65899226 ID=Msa0218630-mRNA-1:exon:18491;Parent=Msa0218630-mRNA-1
chr2_1 exon 65899755 65899824 65899755 ID=Msa0218630-mRNA-1:exon:18492;Parent=Msa0218630-mRNA-1
chr2_1 exon 65900566 65901780 65900566 ID=Msa0218630-mRNA-1:exon:18493;Parent=Msa0218630-mRNA-1
chr2_1 exon 65901869 65902827 65901869 ID=Msa0218630-mRNA-1:exon:18494;Parent=Msa0218630-mRNA-1
chr2_1 five_prime_UTR 65899226 65899484 65899226 ID=Msa0218630-mRNA-1:five_prime_utr;Parent=Msa0218630-mRNA-1
chr2_1 CDS 65899485 65899534 65899485 ID=Msa0218630-mRNA-1:cds;Parent=Msa0218630-mRNA-1
chr2_1 CDS 65899755 65899824 65899755 ID=Msa0218630-mRNA-1:cds;Parent=Msa0218630-mRNA-1
chr2_1 CDS 65900566 65901780 65900566 ID=Msa0218630-mRNA-1:cds;Parent=Msa0218630-mRNA-1
chr2_1 CDS 65901869 65902438 65901869 ID=Msa0218630-mRNA-1:cds;Parent=Msa0218630-mRNA-1
chr2_1 three_prime_UTR 65902439 65902827 65902439 ID=Msa0218630-mRNA-1:three_prime_utr;Parent=Msa0218630-mRNA-1
Gene Sequence

>Msa0218630

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGTCATCACTGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACTGTGTCAATTGATGGTGTCAATTTCCATCTTCACAAGTTTCCTCTTGTTTCAAGATGTGGAAAAATATCCCGTACCTATGAAGATTCCAAGAATACCTATGAGAAGAACCTGACAGTGGTGCTGGAAGAATTTCCTGGGGGTCCTGAAACATTCTTGATTGTGGCCAAATTCTGTTACGGGTTTCGTGTAGAGTTGACAGCAAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGACGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAATATATTGCGCAACTGGAAAGATTGTATTTTGGCTCTCCAAAGCTCCGAGCCTATTCTATCAAGAGCTGAAAATCTTCATTTAGTGGATAAGTGTTTAAACGCTCTATCTATGATGGCTTGCACGGACCCGAGTTTATTTGGATGGCCGATGATGATGTATGGAAGTTTTCAAAGCCCTGGTGGAAGCATTCTTTGGAATGGTATAAATACCGGCGCTAGGATTCGGACCTCAGAATCCGAGTGGTGGTTTGAAGACATCTCATATCTCAGTGTGAGATTGTTTGAGAGCCTTATTAAAACGATGCGAGCGAGAGGTATGAGACCCGAAAGCCTTGCAGGTGCCATAATGTATTATTCTAGGAAGCACTTGCCAGGTCTTGGTCGGTGGCAGGGTGGACAAGGTGGCAAAACAAGAACAGTTGCAAGCTTTAGTTTGACGCCTGCTAGTGCTGCCGTTGATCAGAGGGTTCTTTTGGAAAGCATTGAGAAACTTCTTCCTCAAAAGAAGGGAAAATCTTTTTGCCGTTTCTTACTCGGACTTCTTCGTGTAGCTTCGATATTGAATGTTGATCAAGCTTGCCAGGAATCTTTGGAGAGAAGAATAGGGATGCAACTGGAACTTGCAACATTAGATAGTCTTCTCATTCCTGCTTATGCAGATTCTGATGCATTGTATAACACAGATTGTATTGAACATATTGTCCATCATTTTGTACTTATGGAATCAAATTTAACTGCCTTTTCTCCTTCATCACTTGATCCGCAAGCATCATCGTCGTCTGAATCGTTAAGGAAAGTTGCGAAGTTGATAGATAGCTATATTGGCGAAATTGCGTCTGATGTTAATTTAAAACCAGAAAAGTTATGTGCTCTTGCACAAGCCCTTCCTGAGTCATCGAGATCATTGCATGACGGACTCTACCGAGCACTAGACATATATTTCAAGGCTCATCCTTGGCTCTCTGATAACGAGAAAGAAGAAATATGCAACATCATTGATTACCAAAAACTCTCAATCCATGCATGTGCTCATGCATCTCAAAATGATAGGTTACCCCTCAGAGTTGTTCTTCAAGTCTTATTCTTTGAACAGCTGCATTTGAGAACAGCATTAGCCGGATGTGTCAATGCTTTGGATGGCGAAAGCGCTCCAGCAGTTCCTGCTCGATCGACTGCTCGAGGTGGTACGGCTGGAGAAATTGTACAAAGAAACGGATGGGTTGCTGTTGTGCGCGAAAATCAGGGTTTGAAGGTGGATATGGATAGAATGAGTACTAGAGTCGGAGAACTTGAAGAAGAATTCAGTAAAATAAAAAAGGAAATGAAAACCGTGACCAAATCTCACAGTGCACGTGGTTCTCCTCGGTTACTTGCAAGAAAACTCGGGTGCAAGCTTGTTCCTAGATTTTCAGATGCTCAACCTGAACCCCTTGATCGTTCTGTATCCACACCAAGAGCATCAGTCGAGCGGGCACGCCGTTCCCATAAGTCCAGACACACAGAAAGTTTTTCTTGA
Protein sequence

>Msa0218630

MAPPGKLSGFHLQGHHWFSDSGLPSDITVSIDGVNFHLHKFPLVSRCGKISRTYEDSKNTYEKNLTVVLEEFPGGPETFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNILRNWKDCILALQSSEPILSRAENLHLVDKCLNALSMMACTDPSLFGWPMMMYGSFQSPGGSILWNGINTGARIRTSESEWWFEDISYLSVRLFESLIKTMRARGMRPESLAGAIMYYSRKHLPGLGRWQGGQGGKTRTVASFSLTPASAAVDQRVLLESIEKLLPQKKGKSFCRFLLGLLRVASILNVDQACQESLERRIGMQLELATLDSLLIPAYADSDALYNTDCIEHIVHHFVLMESNLTAFSPSSLDPQASSSSESLRKVAKLIDSYIGEIASDVNLKPEKLCALAQALPESSRSLHDGLYRALDIYFKAHPWLSDNEKEEICNIIDYQKLSIHACAHASQNDRLPLRVVLQVLFFEQLHLRTALAGCVNALDGESAPAVPARSTARGGTAGEIVQRNGWVAVVRENQGLKVDMDRMSTRVGELEEEFSKIKKEMKTVTKSHSARGSPRLLARKLGCKLVPRFSDAQPEPLDRSVSTPRASVERARRSHKSRHTESFS*