AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0297350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0297350 MtrunA17_Chr2g0320691 97.950 634 13 0 1 634 1 634 0.0 1288
Msa0297350 MtrunA17_Chr2g0303541 46.809 611 298 10 6 603 9 605 0.0 572
Msa0297350 MtrunA17_Chr8g0374011 39.130 667 340 17 13 629 10 660 7.11e-152 453
Msa0297350 MtrunA17_Chr4g0050021 37.397 607 332 10 6 576 27 621 4.06e-140 423
Msa0297350 MtrunA17_Chr1g0199631 38.879 589 322 10 41 619 8 568 2.48e-139 418
Msa0297350 MtrunA17_Chr2g0285511 37.500 584 325 7 6 577 9 564 4.11e-138 416
Msa0297350 MtrunA17_Chr2g0279481 36.334 622 339 11 6 583 27 635 5.16e-127 390
Msa0297350 MtrunA17_Chr1g0181181 36.444 568 313 8 10 570 13 539 2.77e-126 384
Msa0297350 MtrunA17_Chr4g0010091 36.101 554 325 7 23 571 34 563 1.18e-120 371
Msa0297350 MtrunA17_Chr5g0401451 35.556 585 338 13 7 584 24 576 5.72e-115 357
Msa0297350 MtrunA17_Chr7g0245201 33.710 620 356 14 18 631 58 628 2.43e-109 343
Msa0297350 MtrunA17_Chr7g0268781 34.524 588 337 12 21 591 34 590 8.86e-105 330
Msa0297350 MtrunA17_Chr8g0382231 30.678 590 334 10 15 582 44 580 3.75e-103 326
Msa0297350 MtrunA17_Chr1g0195911 34.175 594 328 13 21 591 34 587 8.11e-102 322
Msa0297350 MtrunA17_Chr5g0401341 38.716 483 259 9 23 495 27 482 1.82e-101 322
Msa0297350 MtrunA17_Chr4g0053841 32.949 607 365 15 16 609 6 583 2.40e-100 318
Msa0297350 MtrunA17_Chr1g0207461 33.569 566 320 10 10 546 13 551 3.25e-96 308
Msa0297350 MtrunA17_Chr1g0150191 34.719 481 290 6 21 495 25 487 5.12e-95 305
Msa0297350 MtrunA17_Chr3g0108331 32.149 563 326 10 2 563 3 510 1.14e-91 294
Msa0297350 MtrunA17_Chr5g0432861 33.114 607 331 12 4 597 6 550 2.71e-88 286
Msa0297350 MtrunA17_Chr8g0367891 33.024 539 295 10 26 536 29 529 1.36e-87 285
Msa0297350 MtrunA17_Chr4g0076521 34.874 476 282 9 21 494 24 473 9.75e-87 282
Msa0297350 MtrunA17_Chr5g0412591 34.800 500 264 10 21 494 24 487 8.70e-86 280
Msa0297350 MtrunA17_Chr1g0191711 28.445 566 338 8 13 576 48 548 3.07e-82 269
Msa0297350 MtrunA17_Chr2g0290741 32.211 475 286 10 21 494 24 463 1.83e-72 244
Msa0297350 MtrunA17_Chr4g0023801 27.883 581 349 18 20 580 2 532 1.32e-55 197
Msa0297350 MtrunA17_Chr4g0003891 36.177 293 162 4 209 497 43 314 1.12e-53 190
Msa0297350 MtrunA17_Chr4g0037661 27.475 495 309 10 26 500 6 470 2.84e-51 186
Msa0297350 MtrunA17_Chr1g0163311 25.308 569 334 18 36 576 17 522 3.13e-49 179
Msa0297350 MtrunA17_Chr2g0332281 34.843 287 170 3 212 497 24 294 4.02e-48 174
Msa0297350 MtrunA17_Chr2g0280541 26.055 403 242 10 91 482 1 358 1.08e-31 128
Msa0297350 MtrunA17_Chr1g0206621 33.333 138 92 0 31 168 76 213 3.44e-23 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0297350 AT3G44820.1 64.067 654 209 11 1 634 1 648 0.0 829
Msa0297350 AT1G30440.1 45.455 594 302 7 6 583 9 596 0.0 533
Msa0297350 AT5G03250.1 40.820 610 298 12 6 594 9 576 2.04e-150 447
Msa0297350 AT5G13600.1 40.375 587 296 15 10 582 13 559 1.12e-141 425
Msa0297350 AT5G66560.1 37.838 666 321 15 13 603 9 656 1.76e-138 419
Msa0297350 AT5G64330.1 34.493 690 344 14 6 600 35 711 3.88e-130 400
Msa0297350 AT3G50840.1 37.856 597 310 13 17 603 10 555 7.96e-129 391
Msa0297350 AT5G66560.3 37.054 645 313 14 34 603 1 627 1.07e-128 393
Msa0297350 AT5G66560.4 40.000 570 291 13 67 603 66 617 6.70e-127 388
Msa0297350 AT5G64330.2 36.498 611 285 12 6 521 35 637 6.48e-125 384
Msa0297350 AT5G48800.1 35.802 567 316 9 23 581 41 567 1.03e-116 361
Msa0297350 AT3G50840.2 37.798 545 283 11 67 603 23 519 7.26e-115 354
Msa0297350 AT3G50840.3 37.798 545 283 11 67 603 23 519 7.26e-115 354
Msa0297350 AT5G03250.2 38.552 511 241 12 110 594 1 464 1.42e-110 341
Msa0297350 AT5G64330.3 35.456 581 272 12 36 521 6 578 3.18e-110 344
Msa0297350 AT3G08570.1 34.574 564 325 9 18 571 31 560 9.68e-108 338
Msa0297350 AT3G08660.1 34.938 561 299 11 18 571 31 532 1.90e-106 334
Msa0297350 AT5G67385.1 33.333 549 332 8 7 553 9 525 9.88e-103 325
Msa0297350 AT1G03010.3 33.025 648 377 14 7 632 21 633 2.63e-101 322
Msa0297350 AT1G03010.2 33.123 634 369 13 21 632 7 607 5.01e-101 320
Msa0297350 AT1G03010.1 33.123 634 369 13 21 632 34 634 1.22e-100 320
Msa0297350 AT2G14820.2 34.629 566 315 13 1 544 4 536 5.04e-100 318
Msa0297350 AT2G14820.1 34.629 566 315 13 1 544 4 536 5.04e-100 318
Msa0297350 AT2G30520.1 33.394 548 318 8 16 562 23 524 3.34e-97 310
Msa0297350 AT3G08660.3 33.962 530 283 10 49 571 3 472 4.98e-95 302
Msa0297350 AT5G10250.2 30.678 590 336 11 8 573 35 575 5.48e-94 302
Msa0297350 AT5G66560.2 36.735 490 236 11 13 446 9 480 3.36e-93 296
Msa0297350 AT2G47860.1 31.743 608 352 13 21 603 35 604 3.56e-92 298
Msa0297350 AT5G67385.4 32.812 512 310 8 44 553 1 480 5.93e-92 295
Msa0297350 AT5G67385.5 32.812 512 310 8 44 553 1 480 5.93e-92 295
Msa0297350 AT5G67385.2 32.812 512 310 8 44 553 1 480 5.93e-92 295
Msa0297350 AT5G67385.3 32.812 512 310 8 44 553 1 480 5.93e-92 295
Msa0297350 AT5G67440.3 35.611 483 275 8 23 499 27 479 7.86e-92 295
Msa0297350 AT5G67440.1 35.611 483 275 8 23 499 27 479 7.86e-92 295
Msa0297350 AT1G67900.5 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT1G67900.1 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT1G67900.3 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT1G67900.6 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT1G67900.2 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT1G67900.4 37.000 500 273 7 21 494 24 507 2.71e-91 295
Msa0297350 AT5G10250.1 30.743 579 335 11 8 573 35 560 5.76e-91 294
Msa0297350 AT2G30520.2 33.205 521 301 8 43 562 2 476 3.35e-88 285
Msa0297350 AT2G30520.3 33.205 521 301 8 43 562 2 476 3.35e-88 285
Msa0297350 AT5G47800.3 34.810 474 280 8 24 494 27 474 1.71e-87 283
Msa0297350 AT5G47800.1 34.810 474 280 8 24 494 27 474 1.71e-87 283
Msa0297350 AT5G47800.4 34.810 474 280 8 24 494 27 474 1.71e-87 283
Msa0297350 AT2G47860.3 30.586 631 352 14 21 603 35 627 5.63e-87 285
Msa0297350 AT5G47800.2 34.810 474 280 8 24 494 140 587 8.15e-86 282
Msa0297350 AT5G67440.2 35.043 468 268 8 38 499 5 442 2.17e-85 277
Msa0297350 AT4G31820.1 34.086 487 271 12 18 495 22 467 5.17e-84 275
Msa0297350 AT4G37590.1 34.579 535 286 16 1 521 4 488 3.62e-83 273
Msa0297350 AT2G23050.1 34.100 478 251 9 33 503 37 457 7.93e-83 269
Msa0297350 AT3G26490.1 31.423 506 291 7 21 494 24 505 3.23e-80 265
Msa0297350 AT3G26490.3 31.423 506 291 7 21 494 24 505 3.23e-80 265
Msa0297350 AT3G26490.4 31.423 506 291 7 21 494 24 505 3.23e-80 265
Msa0297350 AT3G49970.1 30.037 536 287 13 31 563 2 452 2.50e-74 248
Msa0297350 AT3G49970.2 30.037 536 287 13 31 563 2 452 2.50e-74 248
Msa0297350 AT4G31820.2 33.484 442 248 10 62 495 11 414 8.71e-73 243
Msa0297350 AT3G08660.2 33.028 436 237 8 18 447 31 417 1.28e-72 240
Msa0297350 AT2G47860.5 29.480 519 308 11 110 603 1 486 4.22e-64 220
Msa0297350 AT2G47860.2 29.480 519 308 11 110 603 1 486 4.22e-64 220
Msa0297350 AT2G47860.4 31.767 447 247 10 21 446 35 444 4.76e-63 215
Msa0297350 AT3G26490.2 29.913 458 265 7 21 446 24 457 1.11e-62 216
Msa0297350 AT2G47860.6 30.213 470 247 11 21 446 35 467 3.97e-58 203
Msa0297350 AT2G23050.2 31.156 398 213 8 110 503 1 341 4.74e-57 197
Msa0297350 AT3G19850.1 28.319 452 274 6 65 499 50 468 5.22e-55 196
Msa0297350 AT5G48130.1 25.297 589 372 16 17 590 33 568 1.52e-51 188
Msa0297350 AT1G52770.1 35.395 291 169 4 212 497 28 304 3.77e-50 181
Msa0297350 AT5G17580.1 28.163 490 292 10 25 502 7 448 5.43e-50 182
Msa0297350 AT5G17580.2 28.163 490 292 10 25 502 22 463 6.33e-50 182
Msa0297350 AT1G50280.2 27.602 442 274 11 65 490 16 427 2.18e-48 177
Msa0297350 AT3G22104.1 26.950 564 315 13 25 574 6 486 2.41e-48 177
Msa0297350 AT1G50280.1 27.602 442 274 11 65 490 49 460 3.63e-48 177
Msa0297350 AT3G03510.1 29.062 437 228 11 75 502 4 367 5.97e-47 172
Msa0297350 AT3G15570.1 33.445 299 168 4 212 497 29 309 1.76e-46 171
Msa0297350 AT3G22104.4 26.122 490 268 10 97 574 4 411 1.97e-37 145
Msa0297350 AT3G22104.2 26.122 490 268 10 97 574 4 411 1.97e-37 145
Msa0297350 AT3G22104.3 26.122 490 268 10 97 574 4 411 1.97e-37 145
Msa0297350 AT3G49900.1 30.556 180 108 5 396 569 214 382 1.27e-13 74.3
Msa0297350 AT3G49900.2 29.508 183 109 5 396 569 214 385 3.78e-12 69.7

Find 165 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCACCTGGGAAGCTTTC+TGG 0.046977 2_3:+51031390 Msa0297350:CDS
TTTGGTAGGTTTAGTGATTC+TGG 0.090514 2_3:+51031655 Msa0297350:intron
AACTGAGAATTTGGTTAAAA+AGG 0.164530 2_3:+51031330 Msa0297350:five_prime_UTR
ATGGCACCTGCAAGGCTTTC+GGG 0.203178 2_3:-51033065 None:intergenic
GCCACAATCAAGAATGTTTC+AGG 0.210799 2_3:-51032579 None:intergenic
TTTCTGTGTGTCTGGACTTA+TGG 0.216589 2_3:-51034313 None:intergenic
TGCACGGACCCGAGTTTATT+TGG 0.228486 2_3:+51032861 Msa0297350:CDS
TTAGTATTTAACTGAGAATT+TGG 0.230331 2_3:+51031321 Msa0297350:five_prime_UTR
CTCCACCTGGGAAGCTTTCT+GGG 0.231623 2_3:+51031391 Msa0297350:CDS
GTTCTTCTCATAGGTATTCT+TGG 0.237719 2_3:-51032523 None:intergenic
TGCTGTTGTGCGCGAAAATC+AGG 0.238977 2_3:+51034052 Msa0297350:CDS
ACGAAACCCGTAACAGAATT+TGG 0.245072 2_3:-51032601 None:intergenic
CAACTGGAAAGATTGTATTT+TGG 0.263796 2_3:+51032752 Msa0297350:CDS
CGTTGATCAGAGGGTTCTTT+TGG 0.270046 2_3:+51033196 Msa0297350:CDS
TCAGGGTTTGAAGGTGGATA+TGG 0.270583 2_3:+51034070 Msa0297350:CDS
TCAAGCTTGCCAGGAATCTT+TGG 0.296925 2_3:+51033313 Msa0297350:CDS
TATGGCACCTGCAAGGCTTT+CGG 0.307178 2_3:-51033066 None:intergenic
GCCCTGGTGGAAGCATTCTT+TGG 0.309477 2_3:+51032916 Msa0297350:CDS
AATATTGGAGATGCAAAGTT+TGG 0.317613 2_3:+51034714 Msa0297350:three_prime_UTR
ATGAAAATAAAGAGGAAATA+TGG 0.321948 2_3:-51034358 None:intergenic
TTCTGTGTGTCTGGACTTAT+GGG 0.322141 2_3:-51034312 None:intergenic
TTATTTCCTTTGCTTCAAAA+TGG 0.324437 2_3:+51031368 Msa0297350:exon
GCCAGGTCTTGGTCGGTGGC+AGG 0.331550 2_3:+51033112 Msa0297350:CDS
CGTATTCGTCCGTCATTTCA+AGG 0.342271 2_3:-51032674 None:intergenic
CTTTCTGGGTTTCATCTTCA+AGG 0.342709 2_3:+51031405 Msa0297350:CDS
AGCTGAAAATCTTCATTTAG+TGG 0.344621 2_3:+51032806 Msa0297350:CDS
ATAGAATAGGCTCGGAGCTT+TGG 0.362431 2_3:-51032779 None:intergenic
GGTATTCTTGGAATCTTCAT+AGG 0.366317 2_3:-51032511 None:intergenic
GTGCCATAATGTATTATTCT+AGG 0.366832 2_3:+51033081 Msa0297350:CDS
TTCTTCTCTCCAAAGATTCC+TGG 0.369534 2_3:-51033322 None:intergenic
TTGTGGCCAAATTCTGTTAC+GGG 0.375010 2_3:+51032595 Msa0297350:CDS
TTGGTAGGTTTAGTGATTCT+GGG 0.376444 2_3:+51031656 Msa0297350:intron
AGGAAGCACTTGCCAGGTCT+TGG 0.387407 2_3:+51033101 Msa0297350:CDS
ATGAGACCCGAAAGCCTTGC+AGG 0.391213 2_3:+51033059 Msa0297350:CDS
GTCGATCGAGCAGGAACTGC+TGG 0.391220 2_3:-51033981 None:intergenic
TAAATACCGGCGCTAGGATT+CGG 0.393615 2_3:+51032946 Msa0297350:CDS
CTTCCTAGAATAATACATTA+TGG 0.393701 2_3:-51033084 None:intergenic
ACCGCGTGCACTGTGAGATT+TGG 0.397256 2_3:-51034165 None:intergenic
GAATCTTTGGAGAGAAGAAT+AGG 0.402865 2_3:+51033326 Msa0297350:CDS
ACAGAACGATCAAGGGGTTC+AGG 0.408900 2_3:-51034251 None:intergenic
GTGGTGCTGGAAGAATTTCC+TGG 0.413339 2_3:+51032552 Msa0297350:CDS
GTGATCAAAAGAAAGAATAT+TGG 0.417030 2_3:+51034699 Msa0297350:three_prime_UTR
GAAACTTCTTCCTCAAAAGA+AGG 0.424762 2_3:+51033229 Msa0297350:CDS
AGCACTAGACATATATTTCA+AGG 0.428043 2_3:+51033667 Msa0297350:CDS
GGTTGAGCATCTGAAAATCT+AGG 0.440908 2_3:-51034230 None:intergenic
CCAGGTCTTGGTCGGTGGCA+GGG 0.442080 2_3:+51033113 Msa0297350:CDS
TTTAAACGCTCTATCTATGA+TGG 0.443227 2_3:+51032836 Msa0297350:CDS
TTCTTTCCCTCATGTAAAGT+TGG 0.443597 2_3:+51034514 Msa0297350:three_prime_UTR
TGCTTCAAAATGGCTCCACC+TGG 0.443870 2_3:+51031378 Msa0297350:exon
ATTCCATAAGTACAAAATGA+TGG 0.452056 2_3:-51033445 None:intergenic
CGGTTACTTGCAAGAAAACT+CGG 0.454375 2_3:+51034194 Msa0297350:CDS
CACTGTCAGGTTCTTCTCAT+AGG 0.456166 2_3:-51032532 None:intergenic
CTTTGGAATGGTATAAATAC+CGG 0.458255 2_3:+51032933 Msa0297350:CDS
AATCTTTGGAGAGAAGAATA+GGG 0.459226 2_3:+51033327 Msa0297350:CDS
GATATTACTGTGTCAATTGA+TGG 0.459358 2_3:+51031688 Msa0297350:CDS
ATTGTGGCCAAATTCTGTTA+CGG 0.459464 2_3:+51032594 Msa0297350:CDS
AGCCGGATGTGTCAATGCTT+TGG 0.460297 2_3:+51033944 Msa0297350:CDS
CTCATGCATCTCAAAATGAT+AGG 0.461872 2_3:+51033859 Msa0297350:CDS
TGATCAACGGCAGCACTAGC+AGG 0.464029 2_3:-51033182 None:intergenic
CTTGGAATCTTCATAGGTAC+GGG 0.466842 2_3:-51032505 None:intergenic
AAGTTGATAGATAGCTATAT+TGG 0.467102 2_3:+51033545 Msa0297350:CDS
CATCGTCGTCTGAATCGTTA+AGG 0.468385 2_3:+51033513 Msa0297350:CDS
GATAGAGTGACTGAGATATT+CGG 0.469082 2_3:+51031263 Msa0297350:five_prime_UTR
GAGAAGAATAGGGATGCAAC+TGG 0.469240 2_3:+51033337 Msa0297350:CDS
GAATGTTGATCAAGCTTGCC+AGG 0.470324 2_3:+51033304 Msa0297350:CDS
GGTCGGTGGCAGGGTGGACA+AGG 0.471322 2_3:+51033122 Msa0297350:CDS
GTCAGAAATTAAGTAAAGTC+TGG 0.471560 2_3:+51031285 Msa0297350:five_prime_UTR
TTTCCTCTTGTTTCAAGATG+TGG 0.472009 2_3:+51032474 Msa0297350:CDS
GGGAAGGGAAGCGAGAAGAA+AGG 0.472367 2_3:-51031161 None:intergenic
TATTCTAGGAAGCACTTGCC+AGG 0.478232 2_3:+51033095 Msa0297350:CDS
ATTCTTCCAGCACCACTGTC+AGG 0.483228 2_3:-51032545 None:intergenic
ACACTTTGCAGGCTCATCCT+TGG 0.485150 2_3:+51033766 Msa0297350:intron
TTCATCTTCAAGGCCATCAC+TGG 0.487943 2_3:+51031415 Msa0297350:CDS
CTTTACATGAGGGAAAGAAA+TGG 0.488916 2_3:-51034510 None:intergenic
ATGAATAAATGAAAATAAAG+AGG 0.488926 2_3:-51034366 None:intergenic
GTGTGGATACAGAACGATCA+AGG 0.491533 2_3:-51034259 None:intergenic
TGAAACCCAGAAAGCTTCCC+AGG 0.493717 2_3:-51031396 None:intergenic
TCTTGGAATCTTCATAGGTA+CGG 0.494586 2_3:-51032506 None:intergenic
CGGACCCGAGTTTATTTGGA+TGG 0.499303 2_3:+51032865 Msa0297350:CDS
GCCCGCTCGACTGATGCTCT+TGG 0.504243 2_3:-51034281 None:intergenic
CCTATGTGTTGATAAATACA+AGG 0.512785 2_3:-51034605 None:intergenic
GCATCAGAATCTGCATAAGC+AGG 0.514844 2_3:-51033389 None:intergenic
ATCCAAAGCATTGACACATC+CGG 0.515020 2_3:-51033946 None:intergenic
GAAGAACCTGACAGTGGTGC+TGG 0.515078 2_3:+51032539 Msa0297350:CDS
GGAGAAATTGTACAAAGAAA+CGG 0.517355 2_3:+51034023 Msa0297350:CDS
TCCTGAAACATTCTTGATTG+TGG 0.519192 2_3:+51032578 Msa0297350:CDS
TGGTGCTGGAAGAATTTCCT+GGG 0.521506 2_3:+51032553 Msa0297350:CDS
ATCAAGCGATGAAGGAGAAA+AGG 0.522933 2_3:-51033480 None:intergenic
CATTTGAGAACAGCATTAGC+CGG 0.524224 2_3:+51033927 Msa0297350:CDS
CCCTGCCACCGACCAAGACC+TGG 0.524414 2_3:-51033113 None:intergenic
CTAGTGCTGCCGTTGATCAG+AGG 0.525231 2_3:+51033186 Msa0297350:CDS
GCTTGCGGATCAAGCGATGA+AGG 0.528515 2_3:-51033488 None:intergenic
ACTGCTCGAGGTGGTACGGC+TGG 0.530026 2_3:+51034002 Msa0297350:CDS
AGCTCTTGATAGAATAGGCT+CGG 0.531807 2_3:-51032787 None:intergenic
TTCCAAAGAATGCTTCCACC+AGG 0.532570 2_3:-51032918 None:intergenic
AAGAAAGAAAATACCAGTGA+TGG 0.535137 2_3:-51031428 None:intergenic
ACCAAGAGCATCAGTCGAGC+GGG 0.538088 2_3:+51034280 Msa0297350:CDS
TTTCCACATCTTGAAACAAG+AGG 0.542911 2_3:-51032477 None:intergenic
CCTTGTATTTATCAACACAT+AGG 0.544116 2_3:+51034605 Msa0297350:three_prime_UTR
ACAGTGCACGCGGTTCTCCT+CGG 0.546364 2_3:+51034174 Msa0297350:CDS
GCGCGAAAATCAGGGTTTGA+AGG 0.547646 2_3:+51034061 Msa0297350:CDS
GACTTGAAGAACAACTCTGA+GGG 0.551619 2_3:-51033886 None:intergenic
GGTGGAAGCATTCTTTGGAA+TGG 0.551948 2_3:+51032921 Msa0297350:CDS
TAGTGCTGCCGTTGATCAGA+GGG 0.552150 2_3:+51033187 Msa0297350:CDS
TCTATCTATGATGGCTTGCA+CGG 0.554280 2_3:+51032845 Msa0297350:CDS
AAACTTCTTCCTCAAAAGAA+GGG 0.557294 2_3:+51033230 Msa0297350:CDS
GCTGTTGTGCGCGAAAATCA+GGG 0.560005 2_3:+51034053 Msa0297350:CDS
GGATGGCCGATGATGATGTA+TGG 0.560892 2_3:+51032882 Msa0297350:CDS
ATCGGCCATCCAAATAAACT+CGG 0.566431 2_3:-51032870 None:intergenic
ACTTGCCAGGTCTTGGTCGG+TGG 0.567054 2_3:+51033108 Msa0297350:CDS
AAACCACCACTCGGATTCTG+AGG 0.569410 2_3:-51032970 None:intergenic
ATGATCTCGATGACTCAGGA+AGG 0.570271 2_3:-51033625 None:intergenic
TGCAATGATCTCGATGACTC+AGG 0.571453 2_3:-51033629 None:intergenic
CTCGACTGATGCTCTTGGTG+TGG 0.573680 2_3:-51034276 None:intergenic
ATGGTATAAATACCGGCGCT+AGG 0.573781 2_3:+51032940 Msa0297350:CDS
CGAAAATCAGGGTTTGAAGG+TGG 0.575204 2_3:+51034064 Msa0297350:CDS
CCTCGAGCAGTCGATCGAGC+AGG 0.575521 2_3:-51033990 None:intergenic
TCTCAGTCACTCTATCATCA+TGG 0.578833 2_3:-51031256 None:intergenic
ATCGACTGCTCGAGGTGGTA+CGG 0.579037 2_3:+51033998 Msa0297350:CDS
ATGACGGACGAATACGAAGA+AGG 0.579734 2_3:+51032681 Msa0297350:CDS
GAAGAAGTCCGAGTAAGAAA+CGG 0.583295 2_3:-51033265 None:intergenic
TCCAAAGAATGCTTCCACCA+GGG 0.589108 2_3:-51032917 None:intergenic
CGGTGGCAGGGTGGACAAGG+TGG 0.592330 2_3:+51033125 Msa0297350:CDS
TGTGGATACAGAACGATCAA+GGG 0.592917 2_3:-51034258 None:intergenic
TTCTCGTTATCAGAGAGCCA+AGG 0.599221 2_3:-51033783 None:intergenic
CAAAAGAACCCTCTGATCAA+CGG 0.599347 2_3:-51033195 None:intergenic
AGCACTTGCCAGGTCTTGGT+CGG 0.602120 2_3:+51033105 Msa0297350:CDS
CACCAAGAGCATCAGTCGAG+CGG 0.602703 2_3:+51034279 Msa0297350:CDS
TGAAATATATGTCTAGTGCT+CGG 0.602823 2_3:-51033664 None:intergenic
GCTTCAAAATGGCTCCACCT+GGG 0.603801 2_3:+51031379 Msa0297350:exon
GAGATGCATGAGCACATGCA+TGG 0.604027 2_3:-51033848 None:intergenic
AATTGTACAAAGAAACGGAT+GGG 0.604670 2_3:+51034028 Msa0297350:CDS
TGCAGAATACCTTGAAATGA+CGG 0.605457 2_3:+51032665 Msa0297350:CDS
CCTGCTCGATCGACTGCTCG+AGG 0.610208 2_3:+51033990 Msa0297350:CDS
TGCACTGTGAGATTTGGTCA+CGG 0.613993 2_3:-51034159 None:intergenic
ATTAAAACGATGCGAGCGAG+AGG 0.617106 2_3:+51033035 Msa0297350:CDS
AAACTTCCATACATCATCAT+CGG 0.618818 2_3:-51032888 None:intergenic
GATAGAATGAGTACTAGAGT+CGG 0.620497 2_3:+51034092 Msa0297350:CDS
GGTCTTGGTCGGTGGCAGGG+TGG 0.626378 2_3:+51033116 Msa0297350:CDS
GGATGTGTCAATGCTTTGGA+TGG 0.630355 2_3:+51033948 Msa0297350:CDS
GGACCTCAGAATCCGAGTGG+TGG 0.632921 2_3:+51032967 Msa0297350:CDS
TGATCTCGATGACTCAGGAA+GGG 0.637384 2_3:-51033624 None:intergenic
GGTGCTGGAAGAATTTCCTG+GGG 0.637441 2_3:+51032554 Msa0297350:CDS
TGAGGTCCGAATCCTAGCGC+CGG 0.638429 2_3:-51032952 None:intergenic
GATGTCTTCAAACCACCACT+CGG 0.642289 2_3:-51032979 None:intergenic
AATACATTATGGCACCTGCA+AGG 0.648443 2_3:-51033073 None:intergenic
GGTTACTTGCAAGAAAACTC+GGG 0.650025 2_3:+51034195 Msa0297350:CDS
TCAGACGACGATGATGCTTG+CGG 0.656201 2_3:-51033503 None:intergenic
ATTGACACAGTAATATCACT+TGG 0.657084 2_3:-51031682 None:intergenic
TCGGCCATCCAAATAAACTC+GGG 0.657524 2_3:-51032869 None:intergenic
AAATTGTACAAAGAAACGGA+TGG 0.658614 2_3:+51034027 Msa0297350:CDS
AGACTTGAAGAACAACTCTG+AGG 0.663274 2_3:-51033887 None:intergenic
CTATGTGTTGATAAATACAA+GGG 0.664074 2_3:-51034604 None:intergenic
ACCAAATCTCACAGTGCACG+CGG 0.665622 2_3:+51034164 Msa0297350:CDS
TGTGTCTGGACTTATGGGAA+CGG 0.677659 2_3:-51034307 None:intergenic
TCATCGAGATCATTGCATGA+CGG 0.684043 2_3:+51033635 Msa0297350:CDS
AACCCAGAAAGCTTCCCAGG+TGG 0.686788 2_3:-51031393 None:intergenic
GTGGATACAGAACGATCAAG+GGG 0.686942 2_3:-51034257 None:intergenic
GCTCGATCGACTGCTCGAGG+TGG 0.696171 2_3:+51033993 Msa0297350:CDS
ACTTGAAGAACAACTCTGAG+GGG 0.701967 2_3:-51033885 None:intergenic
GTGCTGGAAGAATTTCCTGG+GGG 0.715345 2_3:+51032555 Msa0297350:CDS
TTCGGACCTCAGAATCCGAG+TGG 0.719040 2_3:+51032964 Msa0297350:CDS
CAAGGTATTCTGCAGCACAG+TGG 0.723725 2_3:-51032656 None:intergenic
ATACACATGAAAGTGTTGTG+TGG 0.736317 2_3:+51034391 Msa0297350:three_prime_UTR
CTATGAGAAGAACCTGACAG+TGG 0.738065 2_3:+51032533 Msa0297350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTATTTATTTATTTAT+TGG + chr2_3:51031479-51031498 Msa0297350:intron 0.0%
!! ATAAAAAAAAAGAAAGAAAA+AGG - chr2_3:51031206-51031225 None:intergenic 10.0%
!!! ATTTTTTTTTCCTGTTTTTT+TGG + chr2_3:51031624-51031643 Msa0297350:intron 15.0%
!! TATTATGAATAGATTATTAG+TGG - chr2_3:51031771-51031790 None:intergenic 15.0%
!! TATTCATAATAATTTGATAG+TGG + chr2_3:51031780-51031799 Msa0297350:intron 15.0%
!!! AAAACTTTTTTACATCAATA+GGG - chr2_3:51031945-51031964 None:intergenic 15.0%
!!! TAAAACTTTTTTACATCAAT+AGG - chr2_3:51031946-51031965 None:intergenic 15.0%
!! AGAATTCAGTAAAATAAAAA+AGG + chr2_3:51034127-51034146 Msa0297350:CDS 15.0%
!! ATGAATAAATGAAAATAAAG+AGG - chr2_3:51034369-51034388 None:intergenic 15.0%
!!! GAGAAAAAAAAAAGTATGAA+GGG - chr2_3:51031184-51031203 None:intergenic 20.0%
!! TTAGTATTTAACTGAGAATT+TGG + chr2_3:51031321-51031340 Msa0297350:five_prime_UTR 20.0%
!! ATTTGGTTAAAAAGGTAAAA+AGG + chr2_3:51031338-51031357 Msa0297350:five_prime_UTR 20.0%
!! AAACAAAATTCCAAAAAAAC+AGG - chr2_3:51031637-51031656 None:intergenic 20.0%
!!! GTTTTTTTGGAATTTTGTTT+TGG + chr2_3:51031637-51031656 Msa0297350:intron 20.0%
!! ACAAAAGAACAAAAATGAAT+AGG - chr2_3:51031848-51031867 None:intergenic 20.0%
!! AAACAAACTAACAGTAAAAA+AGG - chr2_3:51031914-51031933 None:intergenic 20.0%
!!! ACTAAATGCATTTAAAATGT+TGG - chr2_3:51032190-51032209 None:intergenic 20.0%
!!! AGATGAATATTTTGAGATAT+TGG + chr2_3:51032259-51032278 Msa0297350:intron 20.0%
!! GATATTGGAGAAAATTTATT+TGG + chr2_3:51032274-51032293 Msa0297350:intron 20.0%
!! ATGAAAATAAAGAGGAAATA+TGG - chr2_3:51034361-51034380 None:intergenic 20.0%
!!! GTTTAAATTTTTTTGTACGT+GGG + chr2_3:51034413-51034432 Msa0297350:three_prime_UTR 20.0%
!! GCTTAAATAAAAATGCATAA+TGG - chr2_3:51034468-51034487 None:intergenic 20.0%
!!! TTTCTTTGTACATTTTTCAT+TGG + chr2_3:51034540-51034559 Msa0297350:three_prime_UTR 20.0%
!! AAAAAAAAGTATGAAGGGAA+GGG - chr2_3:51031179-51031198 None:intergenic 25.0%
!! AAAAAAAAAGTATGAAGGGA+AGG - chr2_3:51031180-51031199 None:intergenic 25.0%
!! GGAGAAAAAAAAAAGTATGA+AGG - chr2_3:51031185-51031204 None:intergenic 25.0%
! AACTGAGAATTTGGTTAAAA+AGG + chr2_3:51031330-51031349 Msa0297350:five_prime_UTR 25.0%
! TTATTTCCTTTGCTTCAAAA+TGG + chr2_3:51031368-51031387 Msa0297350:exon 25.0%
!!! GAAAATGACATGTTTTTTAG+TGG + chr2_3:51031547-51031566 Msa0297350:intron 25.0%
!!! TTTTGGAATTTTGTTTTGGT+AGG + chr2_3:51031641-51031660 Msa0297350:intron 25.0%
! TAGATTATTAGTGGAAGAAA+AGG - chr2_3:51031762-51031781 None:intergenic 25.0%
!!! TTGGGATTTTTTTTCTTTTG+TGG + chr2_3:51031811-51031830 Msa0297350:intron 25.0%
! TATAAAGTCACACATATGAT+TGG - chr2_3:51031988-51032007 None:intergenic 25.0%
!!! TTTTTGACTCTTTACATTTG+CGG + chr2_3:51032032-51032051 Msa0297350:intron 25.0%
! ATTGGAGAAAATTTATTTGG+AGG + chr2_3:51032277-51032296 Msa0297350:intron 25.0%
! CACGTAATTTATCATATCAA+AGG - chr2_3:51032409-51032428 None:intergenic 25.0%
!!! AGTTGCGCAATATATTTTTA+TGG - chr2_3:51032737-51032756 None:intergenic 25.0%
! ATAAAAATATATTGCGCAAC+TGG + chr2_3:51032736-51032755 Msa0297350:CDS 25.0%
! CTTCCTAGAATAATACATTA+TGG - chr2_3:51033087-51033106 None:intergenic 25.0%
! ATTCCATAAGTACAAAATGA+TGG - chr2_3:51033448-51033467 None:intergenic 25.0%
!!! AAGTTGATAGATAGCTATAT+TGG + chr2_3:51033545-51033564 Msa0297350:CDS 25.0%
!!! GGTTTAAATTTTTTTGTACG+TGG + chr2_3:51034412-51034431 Msa0297350:three_prime_UTR 25.0%
! AAAAAACCAACTTTACATGA+GGG - chr2_3:51034523-51034542 None:intergenic 25.0%
!! CTATGTGTTGATAAATACAA+GGG - chr2_3:51034607-51034626 None:intergenic 25.0%
!! ATTTTAGACATCAATTGTGA+AGG + chr2_3:51034668-51034687 Msa0297350:three_prime_UTR 25.0%
! GTGATCAAAAGAAAGAATAT+TGG + chr2_3:51034699-51034718 Msa0297350:three_prime_UTR 25.0%
GTCAGAAATTAAGTAAAGTC+TGG + chr2_3:51031285-51031304 Msa0297350:five_prime_UTR 30.0%
TGGTTAAAAAGGTAAAAAGG+AGG + chr2_3:51031341-51031360 Msa0297350:five_prime_UTR 30.0%
GGTTAAAAAGGTAAAAAGGA+GGG + chr2_3:51031342-51031361 Msa0297350:five_prime_UTR 30.0%
AAGAAAGAAAATACCAGTGA+TGG - chr2_3:51031431-51031450 None:intergenic 30.0%
ATTGACACAGTAATATCACT+TGG - chr2_3:51031685-51031704 None:intergenic 30.0%
GATATTACTGTGTCAATTGA+TGG + chr2_3:51031688-51031707 Msa0297350:CDS 30.0%
! TGTCAATTTTCATCTTCACA+AGG + chr2_3:51031711-51031730 Msa0297350:CDS 30.0%
! AAAGTAGGAGCACATTAATT+GGG - chr2_3:51031887-51031906 None:intergenic 30.0%
AGTAAAAAAGGTCACAAAGT+AGG - chr2_3:51031902-51031921 None:intergenic 30.0%
TTGTGATCTCTAGTTAATGA+GGG - chr2_3:51032101-51032120 None:intergenic 30.0%
ATTGTGATCTCTAGTTAATG+AGG - chr2_3:51032102-51032121 None:intergenic 30.0%
GTTATGTGGCTACAATTAAA+AGG + chr2_3:51032233-51032252 Msa0297350:intron 30.0%
ATATTTGCACGGTCAAATAA+GGG + chr2_3:51032335-51032354 Msa0297350:intron 30.0%
AATTACGTGAAATGTTGTCT+TGG + chr2_3:51032420-51032439 Msa0297350:intron 30.0%
! CAACTGGAAAGATTGTATTT+TGG + chr2_3:51032752-51032771 Msa0297350:CDS 30.0%
! TTTTCAGCTCTTGATAGAAT+AGG - chr2_3:51032795-51032814 None:intergenic 30.0%
AGCTGAAAATCTTCATTTAG+TGG + chr2_3:51032806-51032825 Msa0297350:CDS 30.0%
TTTAAACGCTCTATCTATGA+TGG + chr2_3:51032836-51032855 Msa0297350:CDS 30.0%
AAACTTCCATACATCATCAT+CGG - chr2_3:51032891-51032910 None:intergenic 30.0%
!! CTTTGGAATGGTATAAATAC+CGG + chr2_3:51032933-51032952 Msa0297350:CDS 30.0%
GTGCCATAATGTATTATTCT+AGG + chr2_3:51033081-51033100 Msa0297350:CDS 30.0%
AAACTTCTTCCTCAAAAGAA+GGG + chr2_3:51033230-51033249 Msa0297350:CDS 30.0%
! AAAGATTTTCCCTTCTTTTG+AGG - chr2_3:51033242-51033261 None:intergenic 30.0%
! AATCTTTGGAGAGAAGAATA+GGG + chr2_3:51033327-51033346 Msa0297350:CDS 30.0%
! TGTCCATCATTTTGTACTTA+TGG + chr2_3:51033442-51033461 Msa0297350:CDS 30.0%
TGAAATATATGTCTAGTGCT+CGG - chr2_3:51033667-51033686 None:intergenic 30.0%
AGCACTAGACATATATTTCA+AGG + chr2_3:51033667-51033686 Msa0297350:CDS 30.0%
GGAGAAATTGTACAAAGAAA+CGG + chr2_3:51034023-51034042 Msa0297350:CDS 30.0%
AAATTGTACAAAGAAACGGA+TGG + chr2_3:51034027-51034046 Msa0297350:CDS 30.0%
AATTGTACAAAGAAACGGAT+GGG + chr2_3:51034028-51034047 Msa0297350:CDS 30.0%
GAAAAAACCAACTTTACATG+AGG - chr2_3:51034524-51034543 None:intergenic 30.0%
!!! ATTGTCTTGTTTTGTTTGTG+TGG + chr2_3:51034574-51034593 Msa0297350:three_prime_UTR 30.0%
!!! TTGTCTTGTTTTGTTTGTGT+GGG + chr2_3:51034575-51034594 Msa0297350:three_prime_UTR 30.0%
! CCTATGTGTTGATAAATACA+AGG - chr2_3:51034608-51034627 None:intergenic 30.0%
CCTTGTATTTATCAACACAT+AGG + chr2_3:51034605-51034624 Msa0297350:three_prime_UTR 30.0%
!! CATTCTTCATTTTGACATAC+TGG + chr2_3:51034630-51034649 Msa0297350:three_prime_UTR 30.0%
GATAGAGTGACTGAGATATT+CGG + chr2_3:51031263-51031282 Msa0297350:five_prime_UTR 35.0%
AGTTGCATGTATGTATGACT+TGG - chr2_3:51031576-51031595 None:intergenic 35.0%
TTTGGTAGGTTTAGTGATTC+TGG + chr2_3:51031655-51031674 Msa0297350:intron 35.0%
TTGGTAGGTTTAGTGATTCT+GGG + chr2_3:51031656-51031675 Msa0297350:intron 35.0%
!! TTTGATAGTGGAGTAGTTGT+TGG + chr2_3:51031792-51031811 Msa0297350:intron 35.0%
!! TTGATAGTGGAGTAGTTGTT+GGG + chr2_3:51031793-51031812 Msa0297350:intron 35.0%
! CAAAGTAGGAGCACATTAAT+TGG - chr2_3:51031888-51031907 None:intergenic 35.0%
TGTGATCTCTAGTTAATGAG+GGG - chr2_3:51032100-51032119 None:intergenic 35.0%
! GTTCACTTGATCTACTGTTT+TGG + chr2_3:51032208-51032227 Msa0297350:intron 35.0%
!!! CTACTGTTTTGGAAGTTATG+TGG + chr2_3:51032219-51032238 Msa0297350:intron 35.0%
GAGAAAATTTATTTGGAGGC+TGG + chr2_3:51032281-51032300 Msa0297350:intron 35.0%
TATTTGACCGTGCAAATATG+CGG - chr2_3:51032334-51032353 None:intergenic 35.0%
CATATTTGCACGGTCAAATA+AGG + chr2_3:51032334-51032353 Msa0297350:intron 35.0%
TATTTGCACGGTCAAATAAG+GGG + chr2_3:51032336-51032355 Msa0297350:intron 35.0%
! TTTCCTCTTGTTTCAAGATG+TGG + chr2_3:51032474-51032493 Msa0297350:CDS 35.0%
TTTCCACATCTTGAAACAAG+AGG - chr2_3:51032480-51032499 None:intergenic 35.0%
TCTTGGAATCTTCATAGGTA+CGG - chr2_3:51032509-51032528 None:intergenic 35.0%
GGTATTCTTGGAATCTTCAT+AGG - chr2_3:51032514-51032533 None:intergenic 35.0%
GTTCTTCTCATAGGTATTCT+TGG - chr2_3:51032526-51032545 None:intergenic 35.0%
TCCTGAAACATTCTTGATTG+TGG + chr2_3:51032578-51032597 Msa0297350:CDS 35.0%
ATTGTGGCCAAATTCTGTTA+CGG + chr2_3:51032594-51032613 Msa0297350:CDS 35.0%
TGCAGAATACCTTGAAATGA+CGG + chr2_3:51032665-51032684 Msa0297350:CDS 35.0%
GAAACTTCTTCCTCAAAAGA+AGG + chr2_3:51033229-51033248 Msa0297350:CDS 35.0%
!! TCTTTTTGCCGTTTCTTACT+CGG + chr2_3:51033257-51033276 Msa0297350:CDS 35.0%
! GAATCTTTGGAGAGAAGAAT+AGG + chr2_3:51033326-51033345 Msa0297350:CDS 35.0%
!! ATGCATGGATTGAGAGTTTT+TGG - chr2_3:51033836-51033855 None:intergenic 35.0%
CTCATGCATCTCAAAATGAT+AGG + chr2_3:51033859-51033878 Msa0297350:CDS 35.0%
GATAGAATGAGTACTAGAGT+CGG + chr2_3:51034092-51034111 Msa0297350:CDS 35.0%
AGAAAAACTTTCTGTGTGTC+TGG - chr2_3:51034324-51034343 None:intergenic 35.0%
!! ATACACATGAAAGTGTTGTG+TGG + chr2_3:51034391-51034410 Msa0297350:three_prime_UTR 35.0%
CTTTACATGAGGGAAAGAAA+TGG - chr2_3:51034513-51034532 None:intergenic 35.0%
TTCTTTCCCTCATGTAAAGT+TGG + chr2_3:51034514-51034533 Msa0297350:three_prime_UTR 35.0%
!!! TGTCTTGTTTTGTTTGTGTG+GGG + chr2_3:51034576-51034595 Msa0297350:three_prime_UTR 35.0%
TCTCAGTCACTCTATCATCA+TGG - chr2_3:51031259-51031278 None:intergenic 40.0%
CTTTCTGGGTTTCATCTTCA+AGG + chr2_3:51031405-51031424 Msa0297350:CDS 40.0%
TGAATTCCCGCATATTTGCA+CGG + chr2_3:51032324-51032343 Msa0297350:intron 40.0%
ATTTGACCGTGCAAATATGC+GGG - chr2_3:51032333-51032352 None:intergenic 40.0%
AAGAGGAAACTGCAGTTTCA+CGG - chr2_3:51032463-51032482 None:intergenic 40.0%
CTTGGAATCTTCATAGGTAC+GGG - chr2_3:51032508-51032527 None:intergenic 40.0%
GCCACAATCAAGAATGTTTC+AGG - chr2_3:51032582-51032601 None:intergenic 40.0%
TTGTGGCCAAATTCTGTTAC+GGG + chr2_3:51032595-51032614 Msa0297350:CDS 40.0%
ACGAAACCCGTAACAGAATT+TGG - chr2_3:51032604-51032623 None:intergenic 40.0%
AGCTCTTGATAGAATAGGCT+CGG - chr2_3:51032790-51032809 None:intergenic 40.0%
! TCTATCTATGATGGCTTGCA+CGG + chr2_3:51032845-51032864 Msa0297350:CDS 40.0%
ATCGGCCATCCAAATAAACT+CGG - chr2_3:51032873-51032892 None:intergenic 40.0%
! TATGGAAGTTTTCAAAGCCC+TGG + chr2_3:51032900-51032919 Msa0297350:CDS 40.0%
!! TCGCTCGCATCGTTTTAATA+AGG - chr2_3:51033034-51033053 None:intergenic 40.0%
AATACATTATGGCACCTGCA+AGG - chr2_3:51033076-51033095 None:intergenic 40.0%
CAAAAGAACCCTCTGATCAA+CGG - chr2_3:51033198-51033217 None:intergenic 40.0%
GAAGAAGTCCGAGTAAGAAA+CGG - chr2_3:51033268-51033287 None:intergenic 40.0%
TTCTTCTCTCCAAAGATTCC+TGG - chr2_3:51033325-51033344 None:intergenic 40.0%
ATCAAGCGATGAAGGAGAAA+AGG - chr2_3:51033483-51033502 None:intergenic 40.0%
TCATCGAGATCATTGCATGA+CGG + chr2_3:51033635-51033654 Msa0297350:CDS 40.0%
ACTTGAAGAACAACTCTGAG+GGG - chr2_3:51033888-51033907 None:intergenic 40.0%
GACTTGAAGAACAACTCTGA+GGG - chr2_3:51033889-51033908 None:intergenic 40.0%
AGACTTGAAGAACAACTCTG+AGG - chr2_3:51033890-51033909 None:intergenic 40.0%
CATTTGAGAACAGCATTAGC+CGG + chr2_3:51033927-51033946 Msa0297350:CDS 40.0%
!! ATCCAAAGCATTGACACATC+CGG - chr2_3:51033949-51033968 None:intergenic 40.0%
! AGTTTTCTTGCAAGTAACCG+AGG - chr2_3:51034194-51034213 None:intergenic 40.0%
CGGTTACTTGCAAGAAAACT+CGG + chr2_3:51034194-51034213 Msa0297350:CDS 40.0%
GGTTACTTGCAAGAAAACTC+GGG + chr2_3:51034195-51034214 Msa0297350:CDS 40.0%
GGTTGAGCATCTGAAAATCT+AGG - chr2_3:51034233-51034252 None:intergenic 40.0%
TGTGGATACAGAACGATCAA+GGG - chr2_3:51034261-51034280 None:intergenic 40.0%
TTCTGTGTGTCTGGACTTAT+GGG - chr2_3:51034315-51034334 None:intergenic 40.0%
TTTCTGTGTGTCTGGACTTA+TGG - chr2_3:51034316-51034335 None:intergenic 40.0%
! TTTTGTACGTGGGAAAGAGA+AGG + chr2_3:51034423-51034442 Msa0297350:three_prime_UTR 40.0%
!!! GTGGAGCCATTTTGAAGCAA+AGG - chr2_3:51031377-51031396 None:intergenic 45.0%
TTCATCTTCAAGGCCATCAC+TGG + chr2_3:51031415-51031434 Msa0297350:CDS 45.0%
CACTGTCAGGTTCTTCTCAT+AGG - chr2_3:51032535-51032554 None:intergenic 45.0%
CTATGAGAAGAACCTGACAG+TGG + chr2_3:51032533-51032552 Msa0297350:CDS 45.0%
! TGGTGCTGGAAGAATTTCCT+GGG + chr2_3:51032553-51032572 Msa0297350:CDS 45.0%
CGTATTCGTCCGTCATTTCA+AGG - chr2_3:51032677-51032696 None:intergenic 45.0%
!! ATGACGGACGAATACGAAGA+AGG + chr2_3:51032681-51032700 Msa0297350:CDS 45.0%
ATAGAATAGGCTCGGAGCTT+TGG - chr2_3:51032782-51032801 None:intergenic 45.0%
TCGGCCATCCAAATAAACTC+GGG - chr2_3:51032872-51032891 None:intergenic 45.0%
TCCAAAGAATGCTTCCACCA+GGG - chr2_3:51032920-51032939 None:intergenic 45.0%
TTCCAAAGAATGCTTCCACC+AGG - chr2_3:51032921-51032940 None:intergenic 45.0%
!! GGTGGAAGCATTCTTTGGAA+TGG + chr2_3:51032921-51032940 Msa0297350:CDS 45.0%
ATGGTATAAATACCGGCGCT+AGG + chr2_3:51032940-51032959 Msa0297350:CDS 45.0%
TAAATACCGGCGCTAGGATT+CGG + chr2_3:51032946-51032965 Msa0297350:CDS 45.0%
GATGTCTTCAAACCACCACT+CGG - chr2_3:51032982-51033001 None:intergenic 45.0%
ATTAAAACGATGCGAGCGAG+AGG + chr2_3:51033035-51033054 Msa0297350:CDS 45.0%
!! TATTCTAGGAAGCACTTGCC+AGG + chr2_3:51033095-51033114 Msa0297350:CDS 45.0%
! CGTTGATCAGAGGGTTCTTT+TGG + chr2_3:51033196-51033215 Msa0297350:CDS 45.0%
GAATGTTGATCAAGCTTGCC+AGG + chr2_3:51033304-51033323 Msa0297350:CDS 45.0%
TCAAGCTTGCCAGGAATCTT+TGG + chr2_3:51033313-51033332 Msa0297350:CDS 45.0%
GAGAAGAATAGGGATGCAAC+TGG + chr2_3:51033337-51033356 Msa0297350:CDS 45.0%
GCATCAGAATCTGCATAAGC+AGG - chr2_3:51033392-51033411 None:intergenic 45.0%
CATCGTCGTCTGAATCGTTA+AGG + chr2_3:51033513-51033532 Msa0297350:CDS 45.0%
!! GCAAGAGCACGTAACTTTTC+TGG - chr2_3:51033599-51033618 None:intergenic 45.0%
TGATCTCGATGACTCAGGAA+GGG - chr2_3:51033627-51033646 None:intergenic 45.0%
ATGATCTCGATGACTCAGGA+AGG - chr2_3:51033628-51033647 None:intergenic 45.0%
TGCAATGATCTCGATGACTC+AGG - chr2_3:51033632-51033651 None:intergenic 45.0%
ATCGATCGAGAATGTAGCTC+AGG - chr2_3:51033702-51033721 None:intergenic 45.0%
GAATGCTTACGACACTTTGC+AGG + chr2_3:51033755-51033774 Msa0297350:intron 45.0%
TTCTCGTTATCAGAGAGCCA+AGG - chr2_3:51033786-51033805 None:intergenic 45.0%
! GGATGTGTCAATGCTTTGGA+TGG + chr2_3:51033948-51033967 Msa0297350:CDS 45.0%
CGAAAATCAGGGTTTGAAGG+TGG + chr2_3:51034064-51034083 Msa0297350:CDS 45.0%
TCAGGGTTTGAAGGTGGATA+TGG + chr2_3:51034070-51034089 Msa0297350:CDS 45.0%
! TGCACTGTGAGATTTGGTCA+CGG - chr2_3:51034162-51034181 None:intergenic 45.0%
GTGGATACAGAACGATCAAG+GGG - chr2_3:51034260-51034279 None:intergenic 45.0%
GTGTGGATACAGAACGATCA+AGG - chr2_3:51034262-51034281 None:intergenic 45.0%
TGTGTCTGGACTTATGGGAA+CGG - chr2_3:51034310-51034329 None:intergenic 45.0%
TGCTTCAAAATGGCTCCACC+TGG + chr2_3:51031378-51031397 Msa0297350:exon 50.0%
GCTTCAAAATGGCTCCACCT+GGG + chr2_3:51031379-51031398 Msa0297350:exon 50.0%
TGAAACCCAGAAAGCTTCCC+AGG - chr2_3:51031399-51031418 None:intergenic 50.0%
ATTCTTCCAGCACCACTGTC+AGG - chr2_3:51032548-51032567 None:intergenic 50.0%
! GTGGTGCTGGAAGAATTTCC+TGG + chr2_3:51032552-51032571 Msa0297350:CDS 50.0%
! GGTGCTGGAAGAATTTCCTG+GGG + chr2_3:51032554-51032573 Msa0297350:CDS 50.0%
GTGCTGGAAGAATTTCCTGG+GGG + chr2_3:51032555-51032574 Msa0297350:CDS 50.0%
AAGAATGTTTCAGGACCCCC+AGG - chr2_3:51032573-51032592 None:intergenic 50.0%
! CAAGGTATTCTGCAGCACAG+TGG - chr2_3:51032659-51032678 None:intergenic 50.0%
TGCACGGACCCGAGTTTATT+TGG + chr2_3:51032861-51032880 Msa0297350:CDS 50.0%
CGGACCCGAGTTTATTTGGA+TGG + chr2_3:51032865-51032884 Msa0297350:CDS 50.0%
! GGATGGCCGATGATGATGTA+TGG + chr2_3:51032882-51032901 Msa0297350:CDS 50.0%
! GGAAGTTTTCAAAGCCCTGG+TGG + chr2_3:51032903-51032922 Msa0297350:CDS 50.0%
AAACCACCACTCGGATTCTG+AGG - chr2_3:51032973-51032992 None:intergenic 50.0%
TATGGCACCTGCAAGGCTTT+CGG - chr2_3:51033069-51033088 None:intergenic 50.0%
TAGTGCTGCCGTTGATCAGA+GGG + chr2_3:51033187-51033206 Msa0297350:CDS 50.0%
TCAGACGACGATGATGCTTG+CGG - chr2_3:51033506-51033525 None:intergenic 50.0%
ACACTTTGCAGGCTCATCCT+TGG + chr2_3:51033766-51033785 Msa0297350:intron 50.0%
GAGATGCATGAGCACATGCA+TGG - chr2_3:51033851-51033870 None:intergenic 50.0%
AGCCGGATGTGTCAATGCTT+TGG + chr2_3:51033944-51033963 Msa0297350:CDS 50.0%
TGCTGTTGTGCGCGAAAATC+AGG + chr2_3:51034052-51034071 Msa0297350:CDS 50.0%
GCTGTTGTGCGCGAAAATCA+GGG + chr2_3:51034053-51034072 Msa0297350:CDS 50.0%
GCGCGAAAATCAGGGTTTGA+AGG + chr2_3:51034061-51034080 Msa0297350:CDS 50.0%
ACCAAATCTCACAGTGCACG+CGG + chr2_3:51034164-51034183 Msa0297350:CDS 50.0%
ACAGAACGATCAAGGGGTTC+AGG - chr2_3:51034254-51034273 None:intergenic 50.0%
GGGAAGGGAAGCGAGAAGAA+AGG - chr2_3:51031164-51031183 None:intergenic 55.0%
CTCCACCTGGGAAGCTTTCT+GGG + chr2_3:51031391-51031410 Msa0297350:CDS 55.0%
AACCCAGAAAGCTTCCCAGG+TGG - chr2_3:51031396-51031415 None:intergenic 55.0%
GAAGAACCTGACAGTGGTGC+TGG + chr2_3:51032539-51032558 Msa0297350:CDS 55.0%
!! GCCCTGGTGGAAGCATTCTT+TGG + chr2_3:51032916-51032935 Msa0297350:CDS 55.0%
TTCGGACCTCAGAATCCGAG+TGG + chr2_3:51032964-51032983 Msa0297350:CDS 55.0%
ATGAGACCCGAAAGCCTTGC+AGG + chr2_3:51033059-51033078 Msa0297350:CDS 55.0%
ATGGCACCTGCAAGGCTTTC+GGG - chr2_3:51033068-51033087 None:intergenic 55.0%
!! AGGAAGCACTTGCCAGGTCT+TGG + chr2_3:51033101-51033120 Msa0297350:CDS 55.0%
AGCACTTGCCAGGTCTTGGT+CGG + chr2_3:51033105-51033124 Msa0297350:CDS 55.0%
TGATCAACGGCAGCACTAGC+AGG - chr2_3:51033185-51033204 None:intergenic 55.0%
CTAGTGCTGCCGTTGATCAG+AGG + chr2_3:51033186-51033205 Msa0297350:CDS 55.0%
GCTTGCGGATCAAGCGATGA+AGG - chr2_3:51033491-51033510 None:intergenic 55.0%
ATCGACTGCTCGAGGTGGTA+CGG + chr2_3:51033998-51034017 Msa0297350:CDS 55.0%
ACCGCGTGCACTGTGAGATT+TGG - chr2_3:51034168-51034187 None:intergenic 55.0%
CTCGACTGATGCTCTTGGTG+TGG - chr2_3:51034279-51034298 None:intergenic 55.0%
!! CACCAAGAGCATCAGTCGAG+CGG + chr2_3:51034279-51034298 Msa0297350:CDS 55.0%
!! ACCAAGAGCATCAGTCGAGC+GGG + chr2_3:51034280-51034299 Msa0297350:CDS 55.0%
GCTCCACCTGGGAAGCTTTC+TGG + chr2_3:51031390-51031409 Msa0297350:CDS 60.0%
TGAGGTCCGAATCCTAGCGC+CGG - chr2_3:51032955-51032974 None:intergenic 60.0%
GGACCTCAGAATCCGAGTGG+TGG + chr2_3:51032967-51032986 Msa0297350:CDS 60.0%
ACTTGCCAGGTCTTGGTCGG+TGG + chr2_3:51033108-51033127 Msa0297350:CDS 60.0%
GTCGATCGAGCAGGAACTGC+TGG - chr2_3:51033984-51034003 None:intergenic 60.0%
ACAGTGCACGCGGTTCTCCT+CGG + chr2_3:51034174-51034193 Msa0297350:CDS 60.0%
CCAGGTCTTGGTCGGTGGCA+GGG + chr2_3:51033113-51033132 Msa0297350:CDS 65.0%
CCTCGAGCAGTCGATCGAGC+AGG - chr2_3:51033993-51034012 None:intergenic 65.0%
CCTGCTCGATCGACTGCTCG+AGG + chr2_3:51033990-51034009 Msa0297350:CDS 65.0%
GCTCGATCGACTGCTCGAGG+TGG + chr2_3:51033993-51034012 Msa0297350:CDS 65.0%
ACTGCTCGAGGTGGTACGGC+TGG + chr2_3:51034002-51034021 Msa0297350:CDS 65.0%
GCCCGCTCGACTGATGCTCT+TGG - chr2_3:51034284-51034303 None:intergenic 65.0%
GCCAGGTCTTGGTCGGTGGC+AGG + chr2_3:51033112-51033131 Msa0297350:CDS 70.0%
CCCTGCCACCGACCAAGACC+TGG - chr2_3:51033116-51033135 None:intergenic 70.0%
GGTCTTGGTCGGTGGCAGGG+TGG + chr2_3:51033116-51033135 Msa0297350:CDS 70.0%
GGTCGGTGGCAGGGTGGACA+AGG + chr2_3:51033122-51033141 Msa0297350:CDS 70.0%
CGGTGGCAGGGTGGACAAGG+TGG + chr2_3:51033125-51033144 Msa0297350:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 51031130 51034735 51031130 ID=Msa0297350;Name=Msa0297350
chr2_3 mRNA 51031130 51034735 51031130 ID=Msa0297350-mRNA-1;Parent=Msa0297350;Name=Msa0297350-mRNA-1;_AED=0.05;_eAED=0.05;_QI=257|1|1|1|1|1|4|389|634
chr2_3 exon 51031130 51031436 51031130 ID=Msa0297350-mRNA-1:exon:16094;Parent=Msa0297350-mRNA-1
chr2_3 exon 51031663 51031732 51031663 ID=Msa0297350-mRNA-1:exon:16095;Parent=Msa0297350-mRNA-1
chr2_3 exon 51032474 51033688 51032474 ID=Msa0297350-mRNA-1:exon:16096;Parent=Msa0297350-mRNA-1
chr2_3 exon 51033777 51034735 51033777 ID=Msa0297350-mRNA-1:exon:16097;Parent=Msa0297350-mRNA-1
chr2_3 five_prime_UTR 51031130 51031386 51031130 ID=Msa0297350-mRNA-1:five_prime_utr;Parent=Msa0297350-mRNA-1
chr2_3 CDS 51031387 51031436 51031387 ID=Msa0297350-mRNA-1:cds;Parent=Msa0297350-mRNA-1
chr2_3 CDS 51031663 51031732 51031663 ID=Msa0297350-mRNA-1:cds;Parent=Msa0297350-mRNA-1
chr2_3 CDS 51032474 51033688 51032474 ID=Msa0297350-mRNA-1:cds;Parent=Msa0297350-mRNA-1
chr2_3 CDS 51033777 51034346 51033777 ID=Msa0297350-mRNA-1:cds;Parent=Msa0297350-mRNA-1
chr2_3 three_prime_UTR 51034347 51034735 51034347 ID=Msa0297350-mRNA-1:three_prime_utr;Parent=Msa0297350-mRNA-1
Gene Sequence

>Msa0297350

ATGGCTCCACCTGGGAAGCTTTCTGGGTTTCATCTTCAAGGCCATCACTGGTTTAGTGATTCTGGGTTGCCAAGTGATATTACTGTGTCAATTGATGGTGTCAATTTTCATCTTCACAAGTTTCCTCTTGTTTCAAGATGTGGAAAAATATCCCGTACCTATGAAGATTCCAAGAATACCTATGAGAAGAACCTGACAGTGGTGCTGGAAGAATTTCCTGGGGGTCCTGAAACATTCTTGATTGTGGCCAAATTCTGTTACGGGTTTCGTGTAGAGTTGACAGCAAAAAATGTAGTATTAGTCCACTGTGCTGCAGAATACCTTGAAATGACGGACGAATACGAAGAAGGCAACTTGTTGACAAAGTCAGAGAGCTTTTTCCATAAAAATATATTGCGCAACTGGAAAGATTGTATTTTGGCTCTCCAAAGCTCCGAGCCTATTCTATCAAGAGCTGAAAATCTTCATTTAGTGGATAAGTGTTTAAACGCTCTATCTATGATGGCTTGCACGGACCCGAGTTTATTTGGATGGCCGATGATGATGTATGGAAGTTTTCAAAGCCCTGGTGGAAGCATTCTTTGGAATGGTATAAATACCGGCGCTAGGATTCGGACCTCAGAATCCGAGTGGTGGTTTGAAGACATCTCATATCTCAGTGTGAGATTGTTTGAGAGCCTTATTAAAACGATGCGAGCGAGAGGTATGAGACCCGAAAGCCTTGCAGGTGCCATAATGTATTATTCTAGGAAGCACTTGCCAGGTCTTGGTCGGTGGCAGGGTGGACAAGGTGGCAAAACAAGAACAGTTGCAAGCTTTAGTTTGACGCCTGCTAGTGCTGCCGTTGATCAGAGGGTTCTTTTGGAAAGCATTGAGAAACTTCTTCCTCAAAAGAAGGGAAAATCTTTTTGCCGTTTCTTACTCGGACTTCTTCGTGTAGCTTCGATATTGAATGTTGATCAAGCTTGCCAGGAATCTTTGGAGAGAAGAATAGGGATGCAACTGGAACTTGCAACATTAGATAGTCTTCTCATTCCTGCTTATGCAGATTCTGATGCATTGTATAACACAGATTGTATTGAACATATTGTCCATCATTTTGTACTTATGGAATCAAATTTAACTGCCTTTTCTCCTTCATCGCTTGATCCGCAAGCATCATCGTCGTCTGAATCGTTAAGGAAAGTTGCGAAGTTGATAGATAGCTATATTGGCGAAATTGCGTCTGATGTTAATTTAAAACCAGAAAAGTTACGTGCTCTTGCACAAGCCCTTCCTGAGTCATCGAGATCATTGCATGACGGACTCTACCGAGCACTAGACATATATTTCAAGGCTCATCCTTGGCTCTCTGATAACGAGAAAGAAGAAATATGCAACATCATTGATTACCAAAAACTCTCAATCCATGCATGTGCTCATGCATCTCAAAATGATAGGTTACCCCTCAGAGTTGTTCTTCAAGTCTTATTCTTTGAACAGCTGCATTTGAGAACAGCATTAGCCGGATGTGTCAATGCTTTGGATGGCGAAAGCGCTCCAGCAGTTCCTGCTCGATCGACTGCTCGAGGTGGTACGGCTGGAGAAATTGTACAAAGAAACGGATGGGTTGCTGTTGTGCGCGAAAATCAGGGTTTGAAGGTGGATATGGATAGAATGAGTACTAGAGTCGGAGAACTTGAAGAAGAATTCAGTAAAATAAAAAAGGAAATGAAAACCGTGACCAAATCTCACAGTGCACGCGGTTCTCCTCGGTTACTTGCAAGAAAACTCGGGTGCAAGCTTGTTCCTAGATTTTCAGATGCTCAACCTGAACCCCTTGATCGTTCTGTATCCACACCAAGAGCATCAGTCGAGCGGGCACGCCGTTCCCATAAGTCCAGACACACAGAAAGTTTTTCTTGA
Protein sequence

>Msa0297350

MAPPGKLSGFHLQGHHWFSDSGLPSDITVSIDGVNFHLHKFPLVSRCGKISRTYEDSKNTYEKNLTVVLEEFPGGPETFLIVAKFCYGFRVELTAKNVVLVHCAAEYLEMTDEYEEGNLLTKSESFFHKNILRNWKDCILALQSSEPILSRAENLHLVDKCLNALSMMACTDPSLFGWPMMMYGSFQSPGGSILWNGINTGARIRTSESEWWFEDISYLSVRLFESLIKTMRARGMRPESLAGAIMYYSRKHLPGLGRWQGGQGGKTRTVASFSLTPASAAVDQRVLLESIEKLLPQKKGKSFCRFLLGLLRVASILNVDQACQESLERRIGMQLELATLDSLLIPAYADSDALYNTDCIEHIVHHFVLMESNLTAFSPSSLDPQASSSSESLRKVAKLIDSYIGEIASDVNLKPEKLRALAQALPESSRSLHDGLYRALDIYFKAHPWLSDNEKEEICNIIDYQKLSIHACAHASQNDRLPLRVVLQVLFFEQLHLRTALAGCVNALDGESAPAVPARSTARGGTAGEIVQRNGWVAVVRENQGLKVDMDRMSTRVGELEEEFSKIKKEMKTVTKSHSARGSPRLLARKLGCKLVPRFSDAQPEPLDRSVSTPRASVERARRSHKSRHTESFS*