AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0220870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220870 MtrunA17_Chr2g0324961 86.667 285 4 1 1 251 1 285 2.62e-179 493
Msa0220870 MtrunA17_Chr4g0040541 66.436 289 54 6 1 251 1 284 1.51e-124 355
Msa0220870 MtrunA17_Chr4g0046431 55.477 283 79 9 10 250 27 304 4.63e-94 279
Msa0220870 MtrunA17_Chr2g0282871 46.599 294 113 5 1 251 1 293 1.12e-83 252
Msa0220870 MtrunA17_Chr8g0367761 61.026 195 68 2 26 220 46 232 7.85e-78 235
Msa0220870 MtrunA17_Chr8g0389661 59.596 198 34 5 27 183 30 222 3.43e-67 215
Msa0220870 MtrunA17_Chr5g0422431 44.554 202 74 3 20 187 32 229 4.77e-48 164
Msa0220870 MtrunA17_Chr1g0198751 45.026 191 67 3 25 181 37 223 1.78e-47 164
Msa0220870 MtrunA17_Chr7g0227071 43.386 189 69 3 25 178 34 219 1.74e-41 148
Msa0220870 MtrunA17_Chr1g0198741 36.937 111 36 1 105 181 8 118 7.96e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220870 AT4G28500.1 52.313 281 69 8 25 251 36 305 1.24e-85 257
Msa0220870 AT1G28470.1 62.371 194 37 2 24 181 50 243 8.08e-76 233
Msa0220870 AT4G29230.3 57.214 201 39 3 25 183 29 224 1.50e-68 218
Msa0220870 AT4G29230.1 57.214 201 39 3 25 183 29 224 4.77e-68 218
Msa0220870 AT4G29230.2 57.214 201 38 3 25 183 29 223 1.23e-67 217
Msa0220870 AT5G56620.3 54.271 199 42 5 27 180 25 219 2.94e-61 198
Msa0220870 AT5G56620.2 54.271 199 42 5 27 180 25 219 2.99e-61 198
Msa0220870 AT5G56620.1 54.271 199 42 5 27 180 25 219 3.16e-61 197
Msa0220870 AT1G25580.1 47.283 184 63 4 25 179 37 215 1.62e-46 160
Msa0220870 AT3G01600.1 48.507 134 62 2 30 156 30 163 3.04e-32 121
Msa0220870 AT5G14490.2 40.000 190 73 5 30 179 30 218 3.15e-32 119
Msa0220870 AT5G14490.4 40.000 190 73 5 30 179 30 218 5.43e-32 120
Msa0220870 AT5G14490.3 40.000 190 73 5 30 179 33 221 5.67e-32 120
Msa0220870 AT5G14490.1 40.000 190 73 5 30 179 33 221 6.15e-32 120

Find 68 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTAATTCCTCTTACTAA+TGG 0.263928 2_1:+69381746 None:intergenic
TCTTAATTCCTCTTACTAAT+GGG 0.282660 2_1:+69381747 None:intergenic
AACTTGCCACTCACGAAAAC+TGG 0.294833 2_1:+69380616 None:intergenic
TTATACATAGAAATGGAATA+AGG 0.294893 2_1:-69381459 Msa0220870:five_prime_UTR
AGAGGAGAAAGAAGGGGAAT+TGG 0.315805 2_1:-69380489 Msa0220870:CDS
TCAAGAGATTCTTGAACATT+TGG 0.330357 2_1:-69381025 Msa0220870:CDS
CACATTGTCTAGGCTGTGTT+TGG 0.340883 2_1:+69380447 None:intergenic
AATTAACCTGGTAATTTCTC+TGG 0.347710 2_1:+69380907 None:intergenic
AGAAATCACTTGAGCATTGT+TGG 0.356347 2_1:+69380271 None:intergenic
TTCGTGAGTGGCAAGTTAAA+AGG 0.360040 2_1:-69380610 Msa0220870:intron
TAATCATAGTACTATCCTTA+TGG 0.361262 2_1:-69381395 Msa0220870:CDS
CTCTCTCTGTGTCTCCCTCT+TGG 0.363793 2_1:-69381516 Msa0220870:five_prime_UTR
GAAGAACTTGTCTCTTCTAA+AGG 0.383819 2_1:+69381602 None:intergenic
GAGTTGCCTGGATTACCTGC+TGG 0.394171 2_1:-69381069 Msa0220870:CDS
ACAATGAAGAGGAGAAAGAA+GGG 0.411720 2_1:-69380496 Msa0220870:intron
AAGAACTTGTCTCTTCTAAA+GGG 0.412652 2_1:+69381603 None:intergenic
GAATTCATCCCTACTCTTGA+AGG 0.419664 2_1:-69380955 Msa0220870:CDS
GTTAATTAATTATAGGGTGC+TGG 0.422909 2_1:-69381099 Msa0220870:intron
GGGATGAATTCATCAATTAA+AGG 0.427373 2_1:+69380967 None:intergenic
GAAACAAGATGGCACAAAAC+AGG 0.437008 2_1:-69380649 Msa0220870:CDS
TATACATAGAAATGGAATAA+GGG 0.441055 2_1:-69381458 Msa0220870:five_prime_UTR
CTGTGTTTGGTAGAAAACTT+TGG 0.443831 2_1:+69380460 None:intergenic
CCTACTCTTGAAGGAGAGAA+TGG 0.452976 2_1:-69380946 Msa0220870:CDS
TTCTTGTTGAAAGGTGGAAA+GGG 0.456011 2_1:+69381644 None:intergenic
AGTTGCCTGGATTACCTGCT+GGG 0.468652 2_1:-69381068 Msa0220870:CDS
TTCACCCCAGCAGGTAATCC+AGG 0.471003 2_1:+69381063 None:intergenic
AGATAGTTTGAGTTAAGAGA+TGG 0.473289 2_1:+69381539 None:intergenic
TCATGCCACTTCTTGTTGAA+AGG 0.473832 2_1:+69381635 None:intergenic
TCATTCACCCCATTAGTAAG+AGG 0.474578 2_1:-69381755 None:intergenic
GCTGGAATTCATGAGTTGCC+TGG 0.479458 2_1:-69381081 Msa0220870:CDS
TATGCAGGTGTCGGCAAAGA+TGG 0.479967 2_1:-69380763 Msa0220870:intron
CCATTCTCTCCTTCAAGAGT+AGG 0.481831 2_1:+69380946 None:intergenic
TGATCCGACATTTCTTTCAC+CGG 0.491329 2_1:-69380738 Msa0220870:CDS
GGATCAAACTTCACCCCAGC+AGG 0.493343 2_1:+69381054 None:intergenic
CATTCTCTCCTTCAAGAGTA+GGG 0.494352 2_1:+69380947 None:intergenic
TATCTTTGCGTCTCTCCATA+AGG 0.495599 2_1:+69381380 None:intergenic
AACAATGAAGAGGAGAAAGA+AGG 0.499608 2_1:-69380497 Msa0220870:intron
CCATCAAAAGCATACACAAC+AGG 0.501280 2_1:-69380715 Msa0220870:CDS
TCATATCAAATGCCAAGATC+AGG 0.502996 2_1:-69381326 Msa0220870:intron
GAATGTGAAGAACCTGATCT+TGG 0.505797 2_1:+69381314 None:intergenic
TTCCACCTTTCAACAAGAAG+TGG 0.515646 2_1:-69381640 Msa0220870:five_prime_UTR
CTTAATTCCTCTTACTAATG+GGG 0.519058 2_1:+69381748 None:intergenic
CTTCTTGTTGAAAGGTGGAA+AGG 0.524933 2_1:+69381643 None:intergenic
TGCCACTTCTTGTTGAAAGG+TGG 0.541565 2_1:+69381638 None:intergenic
TCAAGAATCTCTTGATCAGT+AGG 0.542994 2_1:+69381033 None:intergenic
ATAGTATTCAACAAATCCAC+TGG 0.553691 2_1:+69380337 None:intergenic
TTCAACAAGAAGTGGCATGA+GGG 0.571306 2_1:-69381632 Msa0220870:five_prime_UTR
GAATGAAAGAAGTACCATCA+TGG 0.572077 2_1:+69380237 None:intergenic
AAAGAAGTACCATCATGGAC+AGG 0.572495 2_1:+69380242 None:intergenic
ATTAGTAAGAGGAATTAAGA+AGG 0.576149 2_1:-69381744 Msa0220870:five_prime_UTR
ACTCATCCAGAGAAATTACC+AGG 0.579439 2_1:-69380913 Msa0220870:intron
TTGATATGATGACCACATGT+AGG 0.596208 2_1:+69381340 None:intergenic
AATGAACCACCACATTGTCT+AGG 0.596526 2_1:+69380437 None:intergenic
GTGGTTAATCATAAAACCAG+TGG 0.602289 2_1:-69380353 Msa0220870:CDS
CAATGAAGAGGAGAAAGAAG+GGG 0.610991 2_1:-69380495 Msa0220870:intron
ATGGCCGGTGAAAGAAATGT+CGG 0.616052 2_1:+69380734 None:intergenic
AACATTTGGAAGCAAAAGTG+AGG 0.616853 2_1:-69381011 Msa0220870:CDS
TCAACAAGAAGTGGCATGAG+GGG 0.619040 2_1:-69381631 Msa0220870:five_prime_UTR
TTTCAACAAGAAGTGGCATG+AGG 0.638930 2_1:-69381633 Msa0220870:five_prime_UTR
ATCAGAACTTGTCCTACATG+TGG 0.643015 2_1:-69381352 Msa0220870:CDS
AAAGTGCACACAGATGCTGA+TGG 0.650930 2_1:-69380676 Msa0220870:CDS
GGCACAAAACAGGTAAAACA+AGG 0.653352 2_1:-69380639 Msa0220870:CDS
CTGATGGAAGTGAAACAAGA+TGG 0.659352 2_1:-69380660 Msa0220870:CDS
CAAACACAGCCTAGACAATG+TGG 0.666262 2_1:-69380446 Msa0220870:CDS
TGATCAAAGTGTTCATGAAG+TGG 0.671005 2_1:-69380372 Msa0220870:CDS
ACATAGAAATGGAATAAGGG+AGG 0.680191 2_1:-69381455 Msa0220870:five_prime_UTR
GTTGCCTGGATTACCTGCTG+GGG 0.708832 2_1:-69381067 Msa0220870:CDS
ACACAGCCTAGACAATGTGG+TGG 0.735435 2_1:-69380443 Msa0220870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTATTATACATAGAAA+TGG - chr2_1:69380513-69380532 Msa0220870:intron 15.0%
!! TACTAAGAATATAAATATTG+TGG - chr2_1:69380856-69380875 Msa0220870:intron 15.0%
!!! TCTTTTATTATACATAGAAA+TGG - chr2_1:69380513-69380532 Msa0220870:intron 15.0%
!! TACTAAGAATATAAATATTG+TGG - chr2_1:69380856-69380875 Msa0220870:intron 15.0%
!!! AAATTCTACCTTACTTTTTT+TGG + chr2_1:69380311-69380330 None:intergenic 20.0%
!! TTATACATAGAAATGGAATA+AGG - chr2_1:69380520-69380539 Msa0220870:intron 20.0%
!! TATACATAGAAATGGAATAA+GGG - chr2_1:69380521-69380540 Msa0220870:intron 20.0%
!!! TAGTACTATGATTATTTTCA+GGG + chr2_1:69380578-69380597 None:intergenic 20.0%
!!! ATAGTACTATGATTATTTTC+AGG + chr2_1:69380579-69380598 None:intergenic 20.0%
!!! ATCATATTGTTATTTTTCCT+TGG - chr2_1:69380800-69380819 Msa0220870:intron 20.0%
!!! TTGTGGTGTTAATTAATTAT+AGG - chr2_1:69380873-69380892 Msa0220870:intron 20.0%
!!! TGTGGTGTTAATTAATTATA+GGG - chr2_1:69380874-69380893 Msa0220870:intron 20.0%
!! TAAAACATGTATAATTAACC+TGG + chr2_1:69381087-69381106 None:intergenic 20.0%
!!! TTTTCTGATAAAAAGCTAAA+TGG - chr2_1:69381584-69381603 Msa0220870:five_prime_UTR 20.0%
!!! AAATTCTACCTTACTTTTTT+TGG + chr2_1:69380311-69380330 None:intergenic 20.0%
!! TTATACATAGAAATGGAATA+AGG - chr2_1:69380520-69380539 Msa0220870:intron 20.0%
!! TATACATAGAAATGGAATAA+GGG - chr2_1:69380521-69380540 Msa0220870:intron 20.0%
!!! TAGTACTATGATTATTTTCA+GGG + chr2_1:69380578-69380597 None:intergenic 20.0%
!!! ATAGTACTATGATTATTTTC+AGG + chr2_1:69380579-69380598 None:intergenic 20.0%
!!! ATCATATTGTTATTTTTCCT+TGG - chr2_1:69380800-69380819 Msa0220870:intron 20.0%
!!! TTGTGGTGTTAATTAATTAT+AGG - chr2_1:69380873-69380892 Msa0220870:intron 20.0%
!!! TGTGGTGTTAATTAATTATA+GGG - chr2_1:69380874-69380893 Msa0220870:intron 20.0%
!! TAAAACATGTATAATTAACC+TGG + chr2_1:69381087-69381106 None:intergenic 20.0%
!!! TTTTCTGATAAAAAGCTAAA+TGG - chr2_1:69381584-69381603 Msa0220870:five_prime_UTR 20.0%
! TCTTAATTCCTCTTACTAAT+GGG + chr2_1:69380235-69380254 None:intergenic 25.0%
! TTCTTAATTCCTCTTACTAA+TGG + chr2_1:69380236-69380255 None:intergenic 25.0%
! ATTAGTAAGAGGAATTAAGA+AGG - chr2_1:69380235-69380254 Msa0220870:CDS 25.0%
! ATCAAGTACCAAAAAAAGTA+AGG - chr2_1:69380300-69380319 Msa0220870:CDS 25.0%
! TAATCATAGTACTATCCTTA+TGG - chr2_1:69380584-69380603 Msa0220870:intron 25.0%
!! TTAAAGCTCTAACAAAATCA+CGG + chr2_1:69380736-69380755 None:intergenic 25.0%
! TAAAATCATTAACATCACCA+AGG + chr2_1:69380820-69380839 None:intergenic 25.0%
! CTATGAAAGAACAAAAGAAA+TGG + chr2_1:69381121-69381140 None:intergenic 25.0%
! ACACAAAAAATGTCATACAT+AGG + chr2_1:69381172-69381191 None:intergenic 25.0%
!! TCTTTCATATCTTTTGATCA+AGG - chr2_1:69381671-69381690 Msa0220870:five_prime_UTR 25.0%
! TCTTAATTCCTCTTACTAAT+GGG + chr2_1:69380235-69380254 None:intergenic 25.0%
! TTCTTAATTCCTCTTACTAA+TGG + chr2_1:69380236-69380255 None:intergenic 25.0%
! ATTAGTAAGAGGAATTAAGA+AGG - chr2_1:69380235-69380254 Msa0220870:CDS 25.0%
! ATCAAGTACCAAAAAAAGTA+AGG - chr2_1:69380300-69380319 Msa0220870:CDS 25.0%
! TAATCATAGTACTATCCTTA+TGG - chr2_1:69380584-69380603 Msa0220870:intron 25.0%
!! TTAAAGCTCTAACAAAATCA+CGG + chr2_1:69380736-69380755 None:intergenic 25.0%
! TAAAATCATTAACATCACCA+AGG + chr2_1:69380820-69380839 None:intergenic 25.0%
! CTATGAAAGAACAAAAGAAA+TGG + chr2_1:69381121-69381140 None:intergenic 25.0%
! ACACAAAAAATGTCATACAT+AGG + chr2_1:69381172-69381191 None:intergenic 25.0%
!! TCTTTCATATCTTTTGATCA+AGG - chr2_1:69381671-69381690 Msa0220870:five_prime_UTR 25.0%
AAGAACTTGTCTCTTCTAAA+GGG + chr2_1:69380379-69380398 None:intergenic 30.0%
! AGATAGTTTGAGTTAAGAGA+TGG + chr2_1:69380443-69380462 None:intergenic 30.0%
! ATTTTCTGTCATCGTGATTT+TGG + chr2_1:69380772-69380791 None:intergenic 30.0%
GTTAATTAATTATAGGGTGC+TGG - chr2_1:69380880-69380899 Msa0220870:intron 30.0%
TCAAGAGATTCTTGAACATT+TGG - chr2_1:69380954-69380973 Msa0220870:CDS 30.0%
GGGATGAATTCATCAATTAA+AGG + chr2_1:69381015-69381034 None:intergenic 30.0%
AATTAACCTGGTAATTTCTC+TGG + chr2_1:69381075-69381094 None:intergenic 30.0%
! ATATTTTACGTCTGATATGC+AGG - chr2_1:69381201-69381220 Msa0220870:intron 30.0%
AAAGCCAGAGAAAACAAATT+GGG - chr2_1:69381436-69381455 Msa0220870:exon 30.0%
!! ATAACCCAATTTGTTTTCTC+TGG + chr2_1:69381443-69381462 None:intergenic 30.0%
ATAGTATTCAACAAATCCAC+TGG + chr2_1:69381645-69381664 None:intergenic 30.0%
AAGAACTTGTCTCTTCTAAA+GGG + chr2_1:69380379-69380398 None:intergenic 30.0%
! AGATAGTTTGAGTTAAGAGA+TGG + chr2_1:69380443-69380462 None:intergenic 30.0%
! ATTTTCTGTCATCGTGATTT+TGG + chr2_1:69380772-69380791 None:intergenic 30.0%
GTTAATTAATTATAGGGTGC+TGG - chr2_1:69380880-69380899 Msa0220870:intron 30.0%
TCAAGAGATTCTTGAACATT+TGG - chr2_1:69380954-69380973 Msa0220870:CDS 30.0%
GGGATGAATTCATCAATTAA+AGG + chr2_1:69381015-69381034 None:intergenic 30.0%
AATTAACCTGGTAATTTCTC+TGG + chr2_1:69381075-69381094 None:intergenic 30.0%
! ATATTTTACGTCTGATATGC+AGG - chr2_1:69381201-69381220 Msa0220870:intron 30.0%
AAAGCCAGAGAAAACAAATT+GGG - chr2_1:69381436-69381455 Msa0220870:exon 30.0%
!! ATAACCCAATTTGTTTTCTC+TGG + chr2_1:69381443-69381462 None:intergenic 30.0%
ATAGTATTCAACAAATCCAC+TGG + chr2_1:69381645-69381664 None:intergenic 30.0%
TTCTTGTTGAAAGGTGGAAA+GGG + chr2_1:69380338-69380357 None:intergenic 35.0%
GAAGAACTTGTCTCTTCTAA+AGG + chr2_1:69380380-69380399 None:intergenic 35.0%
ACATAGAAATGGAATAAGGG+AGG - chr2_1:69380524-69380543 Msa0220870:intron 35.0%
TTGATATGATGACCACATGT+AGG + chr2_1:69380642-69380661 None:intergenic 35.0%
TCATATCAAATGCCAAGATC+AGG - chr2_1:69380653-69380672 Msa0220870:CDS 35.0%
TCAAGAATCTCTTGATCAGT+AGG + chr2_1:69380949-69380968 None:intergenic 35.0%
AACATTTGGAAGCAAAAGTG+AGG - chr2_1:69380968-69380987 Msa0220870:CDS 35.0%
GAAAGCCAGAGAAAACAAAT+TGG - chr2_1:69381435-69381454 Msa0220870:exon 35.0%
CATTGTTTCCAAGATGGTAT+TGG + chr2_1:69381469-69381488 None:intergenic 35.0%
AACAATGAAGAGGAGAAAGA+AGG - chr2_1:69381482-69381501 Msa0220870:five_prime_UTR 35.0%
ACAATGAAGAGGAGAAAGAA+GGG - chr2_1:69381483-69381502 Msa0220870:five_prime_UTR 35.0%
CTGTGTTTGGTAGAAAACTT+TGG + chr2_1:69381522-69381541 None:intergenic 35.0%
! TGATCAAAGTGTTCATGAAG+TGG - chr2_1:69381607-69381626 Msa0220870:five_prime_UTR 35.0%
GTGGTTAATCATAAAACCAG+TGG - chr2_1:69381626-69381645 Msa0220870:five_prime_UTR 35.0%
! AGAAATCACTTGAGCATTGT+TGG + chr2_1:69381711-69381730 None:intergenic 35.0%
!! GAATGAAAGAAGTACCATCA+TGG + chr2_1:69381745-69381764 None:intergenic 35.0%
TTCTTGTTGAAAGGTGGAAA+GGG + chr2_1:69380338-69380357 None:intergenic 35.0%
GAAGAACTTGTCTCTTCTAA+AGG + chr2_1:69380380-69380399 None:intergenic 35.0%
ACATAGAAATGGAATAAGGG+AGG - chr2_1:69380524-69380543 Msa0220870:intron 35.0%
TTGATATGATGACCACATGT+AGG + chr2_1:69380642-69380661 None:intergenic 35.0%
TCATATCAAATGCCAAGATC+AGG - chr2_1:69380653-69380672 Msa0220870:CDS 35.0%
TCAAGAATCTCTTGATCAGT+AGG + chr2_1:69380949-69380968 None:intergenic 35.0%
AACATTTGGAAGCAAAAGTG+AGG - chr2_1:69380968-69380987 Msa0220870:CDS 35.0%
GAAAGCCAGAGAAAACAAAT+TGG - chr2_1:69381435-69381454 Msa0220870:exon 35.0%
CATTGTTTCCAAGATGGTAT+TGG + chr2_1:69381469-69381488 None:intergenic 35.0%
AACAATGAAGAGGAGAAAGA+AGG - chr2_1:69381482-69381501 Msa0220870:five_prime_UTR 35.0%
ACAATGAAGAGGAGAAAGAA+GGG - chr2_1:69381483-69381502 Msa0220870:five_prime_UTR 35.0%
CTGTGTTTGGTAGAAAACTT+TGG + chr2_1:69381522-69381541 None:intergenic 35.0%
! TGATCAAAGTGTTCATGAAG+TGG - chr2_1:69381607-69381626 Msa0220870:five_prime_UTR 35.0%
GTGGTTAATCATAAAACCAG+TGG - chr2_1:69381626-69381645 Msa0220870:five_prime_UTR 35.0%
! AGAAATCACTTGAGCATTGT+TGG + chr2_1:69381711-69381730 None:intergenic 35.0%
!! GAATGAAAGAAGTACCATCA+TGG + chr2_1:69381745-69381764 None:intergenic 35.0%
CTTCTTGTTGAAAGGTGGAA+AGG + chr2_1:69380339-69380358 None:intergenic 40.0%
TTCCACCTTTCAACAAGAAG+TGG - chr2_1:69380339-69380358 Msa0220870:CDS 40.0%
! TCATGCCACTTCTTGTTGAA+AGG + chr2_1:69380347-69380366 None:intergenic 40.0%
TTTCAACAAGAAGTGGCATG+AGG - chr2_1:69380346-69380365 Msa0220870:CDS 40.0%
TTCAACAAGAAGTGGCATGA+GGG - chr2_1:69380347-69380366 Msa0220870:CDS 40.0%
CCAAAAAAGAGAGACCAAGA+GGG + chr2_1:69380480-69380499 None:intergenic 40.0%
ACCAAAAAAGAGAGACCAAG+AGG + chr2_1:69380481-69380500 None:intergenic 40.0%
TATCTTTGCGTCTCTCCATA+AGG + chr2_1:69380602-69380621 None:intergenic 40.0%
ATCAGAACTTGTCCTACATG+TGG - chr2_1:69380627-69380646 Msa0220870:CDS 40.0%
GAATGTGAAGAACCTGATCT+TGG + chr2_1:69380668-69380687 None:intergenic 40.0%
CTCTAACAAAATCACGGTGT+CGG + chr2_1:69380730-69380749 None:intergenic 40.0%
GAATTCATCCCTACTCTTGA+AGG - chr2_1:69381024-69381043 Msa0220870:CDS 40.0%
CATTCTCTCCTTCAAGAGTA+GGG + chr2_1:69381035-69381054 None:intergenic 40.0%
ACTCATCCAGAGAAATTACC+AGG - chr2_1:69381066-69381085 Msa0220870:CDS 40.0%
TGATCCGACATTTCTTTCAC+CGG - chr2_1:69381241-69381260 Msa0220870:intron 40.0%
!! TTGTGTATGCTTTTGATGGC+CGG + chr2_1:69381263-69381282 None:intergenic 40.0%
! CCTGTTGTGTATGCTTTTGA+TGG + chr2_1:69381267-69381286 None:intergenic 40.0%
! CCATCAAAAGCATACACAAC+AGG - chr2_1:69381264-69381283 Msa0220870:intron 40.0%
CTGATGGAAGTGAAACAAGA+TGG - chr2_1:69381319-69381338 Msa0220870:intron 40.0%
GAAACAAGATGGCACAAAAC+AGG - chr2_1:69381330-69381349 Msa0220870:CDS 40.0%
GGCACAAAACAGGTAAAACA+AGG - chr2_1:69381340-69381359 Msa0220870:CDS 40.0%
TTCGTGAGTGGCAAGTTAAA+AGG - chr2_1:69381369-69381388 Msa0220870:CDS 40.0%
GTTATGCACCAATACCATCT+TGG - chr2_1:69381458-69381477 Msa0220870:five_prime_UTR 40.0%
CCTCTTCATTGTTTCCAAGA+TGG + chr2_1:69381475-69381494 None:intergenic 40.0%
CCATCTTGGAAACAATGAAG+AGG - chr2_1:69381472-69381491 Msa0220870:five_prime_UTR 40.0%
CAATGAAGAGGAGAAAGAAG+GGG - chr2_1:69381484-69381503 Msa0220870:five_prime_UTR 40.0%
AATGAACCACCACATTGTCT+AGG + chr2_1:69381545-69381564 None:intergenic 40.0%
! TCTCATTTTCCTGTCCATGA+TGG - chr2_1:69381728-69381747 Msa0220870:five_prime_UTR 40.0%
!! AAAGAAGTACCATCATGGAC+AGG + chr2_1:69381740-69381759 None:intergenic 40.0%
CTTCTTGTTGAAAGGTGGAA+AGG + chr2_1:69380339-69380358 None:intergenic 40.0%
TTCCACCTTTCAACAAGAAG+TGG - chr2_1:69380339-69380358 Msa0220870:CDS 40.0%
! TCATGCCACTTCTTGTTGAA+AGG + chr2_1:69380347-69380366 None:intergenic 40.0%
TTTCAACAAGAAGTGGCATG+AGG - chr2_1:69380346-69380365 Msa0220870:CDS 40.0%
TTCAACAAGAAGTGGCATGA+GGG - chr2_1:69380347-69380366 Msa0220870:CDS 40.0%
CCAAAAAAGAGAGACCAAGA+GGG + chr2_1:69380480-69380499 None:intergenic 40.0%
ACCAAAAAAGAGAGACCAAG+AGG + chr2_1:69380481-69380500 None:intergenic 40.0%
TATCTTTGCGTCTCTCCATA+AGG + chr2_1:69380602-69380621 None:intergenic 40.0%
ATCAGAACTTGTCCTACATG+TGG - chr2_1:69380627-69380646 Msa0220870:CDS 40.0%
GAATGTGAAGAACCTGATCT+TGG + chr2_1:69380668-69380687 None:intergenic 40.0%
CTCTAACAAAATCACGGTGT+CGG + chr2_1:69380730-69380749 None:intergenic 40.0%
GAATTCATCCCTACTCTTGA+AGG - chr2_1:69381024-69381043 Msa0220870:CDS 40.0%
CATTCTCTCCTTCAAGAGTA+GGG + chr2_1:69381035-69381054 None:intergenic 40.0%
ACTCATCCAGAGAAATTACC+AGG - chr2_1:69381066-69381085 Msa0220870:CDS 40.0%
TGATCCGACATTTCTTTCAC+CGG - chr2_1:69381241-69381260 Msa0220870:intron 40.0%
!! TTGTGTATGCTTTTGATGGC+CGG + chr2_1:69381263-69381282 None:intergenic 40.0%
! CCTGTTGTGTATGCTTTTGA+TGG + chr2_1:69381267-69381286 None:intergenic 40.0%
! CCATCAAAAGCATACACAAC+AGG - chr2_1:69381264-69381283 Msa0220870:intron 40.0%
CTGATGGAAGTGAAACAAGA+TGG - chr2_1:69381319-69381338 Msa0220870:intron 40.0%
GAAACAAGATGGCACAAAAC+AGG - chr2_1:69381330-69381349 Msa0220870:CDS 40.0%
GGCACAAAACAGGTAAAACA+AGG - chr2_1:69381340-69381359 Msa0220870:CDS 40.0%
TTCGTGAGTGGCAAGTTAAA+AGG - chr2_1:69381369-69381388 Msa0220870:CDS 40.0%
GTTATGCACCAATACCATCT+TGG - chr2_1:69381458-69381477 Msa0220870:five_prime_UTR 40.0%
CCTCTTCATTGTTTCCAAGA+TGG + chr2_1:69381475-69381494 None:intergenic 40.0%
CCATCTTGGAAACAATGAAG+AGG - chr2_1:69381472-69381491 Msa0220870:five_prime_UTR 40.0%
CAATGAAGAGGAGAAAGAAG+GGG - chr2_1:69381484-69381503 Msa0220870:five_prime_UTR 40.0%
AATGAACCACCACATTGTCT+AGG + chr2_1:69381545-69381564 None:intergenic 40.0%
! TCTCATTTTCCTGTCCATGA+TGG - chr2_1:69381728-69381747 Msa0220870:five_prime_UTR 40.0%
!! AAAGAAGTACCATCATGGAC+AGG + chr2_1:69381740-69381759 None:intergenic 40.0%
! TGCCACTTCTTGTTGAAAGG+TGG + chr2_1:69380344-69380363 None:intergenic 45.0%
TCAACAAGAAGTGGCATGAG+GGG - chr2_1:69380348-69380367 Msa0220870:CDS 45.0%
!!! CCCTCTTGGTCTCTCTTTTT+TGG - chr2_1:69380477-69380496 Msa0220870:CDS 45.0%
AGGTTCTTCACATTCGTGCA+TGG - chr2_1:69380673-69380692 Msa0220870:CDS 45.0%
CCATTCTCTCCTTCAAGAGT+AGG + chr2_1:69381036-69381055 None:intergenic 45.0%
CCTACTCTTGAAGGAGAGAA+TGG - chr2_1:69381033-69381052 Msa0220870:CDS 45.0%
TACGTCTGATATGCAGGTGT+CGG - chr2_1:69381207-69381226 Msa0220870:intron 45.0%
ATGGCCGGTGAAAGAAATGT+CGG + chr2_1:69381248-69381267 None:intergenic 45.0%
AAAGTGCACACAGATGCTGA+TGG - chr2_1:69381303-69381322 Msa0220870:intron 45.0%
AACTTGCCACTCACGAAAAC+TGG + chr2_1:69381366-69381385 None:intergenic 45.0%
AGAGGAGAAAGAAGGGGAAT+TGG - chr2_1:69381490-69381509 Msa0220870:five_prime_UTR 45.0%
CACATTGTCTAGGCTGTGTT+TGG + chr2_1:69381535-69381554 None:intergenic 45.0%
CAAACACAGCCTAGACAATG+TGG - chr2_1:69381533-69381552 Msa0220870:five_prime_UTR 45.0%
! TGCCACTTCTTGTTGAAAGG+TGG + chr2_1:69380344-69380363 None:intergenic 45.0%
TCAACAAGAAGTGGCATGAG+GGG - chr2_1:69380348-69380367 Msa0220870:CDS 45.0%
!!! CCCTCTTGGTCTCTCTTTTT+TGG - chr2_1:69380477-69380496 Msa0220870:CDS 45.0%
AGGTTCTTCACATTCGTGCA+TGG - chr2_1:69380673-69380692 Msa0220870:CDS 45.0%
CCATTCTCTCCTTCAAGAGT+AGG + chr2_1:69381036-69381055 None:intergenic 45.0%
CCTACTCTTGAAGGAGAGAA+TGG - chr2_1:69381033-69381052 Msa0220870:CDS 45.0%
TACGTCTGATATGCAGGTGT+CGG - chr2_1:69381207-69381226 Msa0220870:intron 45.0%
ATGGCCGGTGAAAGAAATGT+CGG + chr2_1:69381248-69381267 None:intergenic 45.0%
AAAGTGCACACAGATGCTGA+TGG - chr2_1:69381303-69381322 Msa0220870:intron 45.0%
AACTTGCCACTCACGAAAAC+TGG + chr2_1:69381366-69381385 None:intergenic 45.0%
AGAGGAGAAAGAAGGGGAAT+TGG - chr2_1:69381490-69381509 Msa0220870:five_prime_UTR 45.0%
CACATTGTCTAGGCTGTGTT+TGG + chr2_1:69381535-69381554 None:intergenic 45.0%
CAAACACAGCCTAGACAATG+TGG - chr2_1:69381533-69381552 Msa0220870:five_prime_UTR 45.0%
GCTGGAATTCATGAGTTGCC+TGG - chr2_1:69380898-69380917 Msa0220870:intron 50.0%
AGTTGCCTGGATTACCTGCT+GGG - chr2_1:69380911-69380930 Msa0220870:intron 50.0%
!! TATGCAGGTGTCGGCAAAGA+TGG - chr2_1:69381216-69381235 Msa0220870:intron 50.0%
! ACAAGGCCAGTTTTCGTGAG+TGG - chr2_1:69381357-69381376 Msa0220870:CDS 50.0%
ACACAGCCTAGACAATGTGG+TGG - chr2_1:69381536-69381555 Msa0220870:five_prime_UTR 50.0%
GCTGGAATTCATGAGTTGCC+TGG - chr2_1:69380898-69380917 Msa0220870:intron 50.0%
AGTTGCCTGGATTACCTGCT+GGG - chr2_1:69380911-69380930 Msa0220870:intron 50.0%
!! TATGCAGGTGTCGGCAAAGA+TGG - chr2_1:69381216-69381235 Msa0220870:intron 50.0%
! ACAAGGCCAGTTTTCGTGAG+TGG - chr2_1:69381357-69381376 Msa0220870:CDS 50.0%
ACACAGCCTAGACAATGTGG+TGG - chr2_1:69381536-69381555 Msa0220870:five_prime_UTR 50.0%
CTCTCTCTGTGTCTCCCTCT+TGG - chr2_1:69380463-69380482 Msa0220870:CDS 55.0%
GAGTTGCCTGGATTACCTGC+TGG - chr2_1:69380910-69380929 Msa0220870:intron 55.0%
GTTGCCTGGATTACCTGCTG+GGG - chr2_1:69380912-69380931 Msa0220870:intron 55.0%
TTCACCCCAGCAGGTAATCC+AGG + chr2_1:69380919-69380938 None:intergenic 55.0%
GGATCAAACTTCACCCCAGC+AGG + chr2_1:69380928-69380947 None:intergenic 55.0%
CTCTCTCTGTGTCTCCCTCT+TGG - chr2_1:69380463-69380482 Msa0220870:CDS 55.0%
GAGTTGCCTGGATTACCTGC+TGG - chr2_1:69380910-69380929 Msa0220870:intron 55.0%
GTTGCCTGGATTACCTGCTG+GGG - chr2_1:69380912-69380931 Msa0220870:intron 55.0%
TTCACCCCAGCAGGTAATCC+AGG + chr2_1:69380919-69380938 None:intergenic 55.0%
GGATCAAACTTCACCCCAGC+AGG + chr2_1:69380928-69380947 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69380232 69381769 69380232 ID=Msa0220870;Name=Msa0220870
chr2_1 mRNA 69380232 69381769 69380232 ID=Msa0220870-mRNA-1;Parent=Msa0220870;Name=Msa0220870-mRNA-1;_AED=0.22;_eAED=0.22;_QI=326|0.66|0.5|1|1|1|4|0|251
chr2_1 exon 69380232 69380509 69380232 ID=Msa0220870-mRNA-1:exon:19670;Parent=Msa0220870-mRNA-1
chr2_1 exon 69380611 69380778 69380611 ID=Msa0220870-mRNA-1:exon:19669;Parent=Msa0220870-mRNA-1
chr2_1 exon 69380914 69381106 69380914 ID=Msa0220870-mRNA-1:exon:19668;Parent=Msa0220870-mRNA-1
chr2_1 exon 69381327 69381769 69381327 ID=Msa0220870-mRNA-1:exon:19667;Parent=Msa0220870-mRNA-1
chr2_1 five_prime_UTR 69381444 69381769 69381444 ID=Msa0220870-mRNA-1:five_prime_utr;Parent=Msa0220870-mRNA-1
chr2_1 CDS 69381327 69381443 69381327 ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1 CDS 69380914 69381106 69380914 ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1 CDS 69380611 69380778 69380611 ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1 CDS 69380232 69380509 69380232 ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
Gene Sequence

>Msa0220870

ATGACTCAGTGTAGTTACCCTGAAAATAATCATAGTACTATCCTTATGGAGAGACGCAAAGATAGCTTAATCAGAACTTGTCCTACATGTGGTCATCATATCAAATGCCAAGATCAGGGTGCTGGAATTCATGAGTTGCCTGGATTACCTGCTGGGGTGAAGTTTGATCCTACTGATCAAGAGATTCTTGAACATTTGGAAGCAAAAGTGAGGTCTGATATTCAAATGCTTCATCCTTTAATTGATGAATTCATCCCTACTCTTGAAGGAGAGAATGGAATCTGCTGTACTCATCCAGAGAAATTACCAGGTGTCGGCAAAGATGGACTGATCCGACATTTCTTTCACCGGCCATCAAAAGCATACACAACAGGAACAAGAAAAAGAAGAAAAGTGCACACAGATGCTGATGGAAGTGAAACAAGATGGCACAAAACAGGTAAAACAAGGCCAGTTTTCGTGAGTGGCAAGTTAAAAGAGGAGAAAGAAGGGGAATTGGTAGTTTCCAAAGTTTTCTACCAAACACAGCCTAGACAATGTGGTGGTTCATTGATGAAAGACTCAGCATCATTTTCTGATAAAAAGCTAAATGGTGATCAAAGTGTTCATGAAGTGGTTAATCATAAAACCAGTGGATTTGTTGAATACTATAGTACTTCTTTCATATCTTTTGATCAAGGTGAACAACATAGATCCAACAATGCTCAAGTGATTTCTCATTTTCCTGTCCATGATGGTACTTCTTTCATTCCTTGA
Protein sequence

>Msa0220870

MTQCSYPENNHSTILMERRKDSLIRTCPTCGHHIKCQDQGAGIHELPGLPAGVKFDPTDQEILEHLEAKVRSDIQMLHPLIDEFIPTLEGENGICCTHPEKLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDADGSETRWHKTGKTRPVFVSGKLKEEKEGELVVSKVFYQTQPRQCGGSLMKDSASFSDKKLNGDQSVHEVVNHKTSGFVEYYSTSFISFDQGEQHRSNNAQVISHFPVHDGTSFIP*