AlfalfaGEDB | Msa0220870

M. sativa cultivar zhongmu-4 / Msa0220870

KEGG: -
GO: GO:0000075 GO:0000077 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006974 GO:0008150 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010564 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031570 GO:0033043 GO:0033554 GO:0040020 GO:0043565 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2000241 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220870	XP_013465160.1	86.667	285	4	1	1	251	1	285	2.85e-175	493

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220870	sp\|O49459\|NAC73_ARATH	52.313	281	69	8	25	251	36	305	1.22e-84	257

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220870	A0A072VM12	86.667	285	4	1	1	251	1	285	1.36e-175	493

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TFs/TRs:

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Gene ID	Type	Classification
Msa0220870	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0220870	Msa0227950	0.924746	4.663938e-90	-8.615850e-47
Msa0220870	Msa0300210	0.897421	1.429384e-76	-8.615850e-47
Msa0220870	Msa0337540	0.830185	3.385268e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220870	MtrunA17_Chr2g0324961	86.667	285	4	1	1	251	1	285	2.62e-179	493
Msa0220870	MtrunA17_Chr4g0040541	66.436	289	54	6	1	251	1	284	1.51e-124	355
Msa0220870	MtrunA17_Chr4g0046431	55.477	283	79	9	10	250	27	304	4.63e-94	279
Msa0220870	MtrunA17_Chr2g0282871	46.599	294	113	5	1	251	1	293	1.12e-83	252
Msa0220870	MtrunA17_Chr8g0367761	61.026	195	68	2	26	220	46	232	7.85e-78	235
Msa0220870	MtrunA17_Chr8g0389661	59.596	198	34	5	27	183	30	222	3.43e-67	215
Msa0220870	MtrunA17_Chr5g0422431	44.554	202	74	3	20	187	32	229	4.77e-48	164
Msa0220870	MtrunA17_Chr1g0198751	45.026	191	67	3	25	181	37	223	1.78e-47	164
Msa0220870	MtrunA17_Chr7g0227071	43.386	189	69	3	25	178	34	219	1.74e-41	148
Msa0220870	MtrunA17_Chr1g0198741	36.937	111	36	1	105	181	8	118	7.96e-15	72.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220870	AT4G28500.1	52.313	281	69	8	25	251	36	305	1.24e-85	257
Msa0220870	AT1G28470.1	62.371	194	37	2	24	181	50	243	8.08e-76	233
Msa0220870	AT4G29230.3	57.214	201	39	3	25	183	29	224	1.50e-68	218
Msa0220870	AT4G29230.1	57.214	201	39	3	25	183	29	224	4.77e-68	218
Msa0220870	AT4G29230.2	57.214	201	38	3	25	183	29	223	1.23e-67	217
Msa0220870	AT5G56620.3	54.271	199	42	5	27	180	25	219	2.94e-61	198
Msa0220870	AT5G56620.2	54.271	199	42	5	27	180	25	219	2.99e-61	198
Msa0220870	AT5G56620.1	54.271	199	42	5	27	180	25	219	3.16e-61	197
Msa0220870	AT1G25580.1	47.283	184	63	4	25	179	37	215	1.62e-46	160
Msa0220870	AT3G01600.1	48.507	134	62	2	30	156	30	163	3.04e-32	121

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Find 68 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGTACCATCATGGAC+AGG	0.572495	2_1:+69380242	None:intergenic
AAAGTGCACACAGATGCTGA+TGG	0.650930	2_1:-69380676	Msa0220870:CDS
AACAATGAAGAGGAGAAAGA+AGG	0.499608	2_1:-69380497	Msa0220870:intron
AACATTTGGAAGCAAAAGTG+AGG	0.616853	2_1:-69381011	Msa0220870:CDS
AACTTGCCACTCACGAAAAC+TGG	0.294833	2_1:+69380616	None:intergenic
AAGAACTTGTCTCTTCTAAA+GGG	0.412652	2_1:+69381603	None:intergenic
AATGAACCACCACATTGTCT+AGG	0.596526	2_1:+69380437	None:intergenic
AATTAACCTGGTAATTTCTC+TGG	0.347710	2_1:+69380907	None:intergenic
ACAATGAAGAGGAGAAAGAA+GGG	0.411720	2_1:-69380496	Msa0220870:intron
ACACAGCCTAGACAATGTGG+TGG	0.735435	2_1:-69380443	Msa0220870:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAACTTGTCTCTTCTAAA+GGG	+	chr2_1:69380379-69380398	None:intergenic	30.0%
GTTAATTAATTATAGGGTGC+TGG	-	chr2_1:69380880-69380899	Msa0220870:intron	30.0%
TCAAGAGATTCTTGAACATT+TGG	-	chr2_1:69380954-69380973	Msa0220870:CDS	30.0%
GGGATGAATTCATCAATTAA+AGG	+	chr2_1:69381015-69381034	None:intergenic	30.0%
AATTAACCTGGTAATTTCTC+TGG	+	chr2_1:69381075-69381094	None:intergenic	30.0%
AAAGCCAGAGAAAACAAATT+GGG	-	chr2_1:69381436-69381455	Msa0220870:exon	30.0%
ATAGTATTCAACAAATCCAC+TGG	+	chr2_1:69381645-69381664	None:intergenic	30.0%
AAGAACTTGTCTCTTCTAAA+GGG	+	chr2_1:69380379-69380398	None:intergenic	30.0%
GTTAATTAATTATAGGGTGC+TGG	-	chr2_1:69380880-69380899	Msa0220870:intron	30.0%
TCAAGAGATTCTTGAACATT+TGG	-	chr2_1:69380954-69380973	Msa0220870:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	69380232	69381769	69380232	ID=Msa0220870;Name=Msa0220870
chr2_1	mRNA	69380232	69381769	69380232	ID=Msa0220870-mRNA-1;Parent=Msa0220870;Name=Msa0220870-mRNA-1;_AED=0.22;_eAED=0.22;_QI=326\|0.66\|0.5\|1\|1\|1\|4\|0\|251
chr2_1	exon	69380232	69380509	69380232	ID=Msa0220870-mRNA-1:exon:19670;Parent=Msa0220870-mRNA-1
chr2_1	exon	69380611	69380778	69380611	ID=Msa0220870-mRNA-1:exon:19669;Parent=Msa0220870-mRNA-1
chr2_1	exon	69380914	69381106	69380914	ID=Msa0220870-mRNA-1:exon:19668;Parent=Msa0220870-mRNA-1
chr2_1	exon	69381327	69381769	69381327	ID=Msa0220870-mRNA-1:exon:19667;Parent=Msa0220870-mRNA-1
chr2_1	five_prime_UTR	69381444	69381769	69381444	ID=Msa0220870-mRNA-1:five_prime_utr;Parent=Msa0220870-mRNA-1
chr2_1	CDS	69381327	69381443	69381327	ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1	CDS	69380914	69381106	69380914	ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1	CDS	69380611	69380778	69380611	ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1
chr2_1	CDS	69380232	69380509	69380232	ID=Msa0220870-mRNA-1:cds;Parent=Msa0220870-mRNA-1

Gene Sequence

>Msa0220870

ATGACTCAGTGTAGTTACCCTGAAAATAATCATAGTACTATCCTTATGGAGAGACGCAAAGATAGCTTAATCAGAACTTGTCCTACATGTGGTCATCATATCAAATGCCAAGATCAGGGTGCTGGAATTCATGAGTTGCCTGGATTACCTGCTGGGGTGAAGTTTGATCCTACTGATCAAGAGATTCTTGAACATTTGGAAGCAAAAGTGAGGTCTGATATTCAAATGCTTCATCCTTTAATTGATGAATTCATCCCTACTCTTGAAGGAGAGAATGGAATCTGCTGTACTCATCCAGAGAAATTACCAGGTGTCGGCAAAGATGGACTGATCCGACATTTCTTTCACCGGCCATCAAAAGCATACACAACAGGAACAAGAAAAAGAAGAAAAGTGCACACAGATGCTGATGGAAGTGAAACAAGATGGCACAAAACAGGTAAAACAAGGCCAGTTTTCGTGAGTGGCAAGTTAAAAGAGGAGAAAGAAGGGGAATTGGTAGTTTCCAAAGTTTTCTACCAAACACAGCCTAGACAATGTGGTGGTTCATTGATGAAAGACTCAGCATCATTTTCTGATAAAAAGCTAAATGGTGATCAAAGTGTTCATGAAGTGGTTAATCATAAAACCAGTGGATTTGTTGAATACTATAGTACTTCTTTCATATCTTTTGATCAAGGTGAACAACATAGATCCAACAATGCTCAAGTGATTTCTCATTTTCCTGTCCATGATGGTACTTCTTTCATTCCTTGA

Protein sequence

>Msa0220870

MTQCSYPENNHSTILMERRKDSLIRTCPTCGHHIKCQDQGAGIHELPGLPAGVKFDPTDQEILEHLEAKVRSDIQMLHPLIDEFIPTLEGENGICCTHPEKLPGVGKDGLIRHFFHRPSKAYTTGTRKRRKVHTDADGSETRWHKTGKTRPVFVSGKLKEEKEGELVVSKVFYQTQPRQCGGSLMKDSASFSDKKLNGDQSVHEVVNHKTSGFVEYYSTSFISFDQGEQHRSNNAQVISHFPVHDGTSFIP*