AlfalfaGEDB | Msa0222100

M. sativa cultivar zhongmu-4 / Msa0222100

KEGG: K15148
GO: GO:0000122 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016591 GO:0016592 GO:0019219 GO:0019222 GO:0030880 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070847 GO:0080090 GO:0090575 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1990234 GO:2000112 GO:2000113 GO:2001141
PFAM: PF05983.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222100	XP_013452663.1	100.000	168	0	0	6	173	1	168	3.85e-118	341

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222100	sp\|Q9LZD7\|MED7B_ARATH	77.976	168	37	0	6	173	1	168	2.69e-88	258

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222100	A0A072UB69	100.000	168	0	0	6	173	1	168	1.84e-118	341

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TFs/TRs:

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Gene ID	Type	Classification
Msa0222100	TR	MED7

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0222050	Msa0222100	0.963321	6.088473e-122	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222100	MtrunA17_Chr6g0478701	100.000	168	0	0	6	173	1	168	3.54e-122	341
Msa0222100	MtrunA17_Chr2g0326811	99.405	168	1	0	6	173	1	168	1.39e-121	340

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0222100	AT5G03500.6	77.515	169	38	5	173	10	178	1.97e-89	259
Msa0222100	AT5G03500.3	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03500.4	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03500.2	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03500.5	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03500.7	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03500.1	77.976	168	37	6	173	1	168	2.73e-89	258
Msa0222100	AT5G03220.1	79.167	168	35	6	173	1	168	2.30e-84	246

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Find 54 sgRNAs with CRISPR-Local

Find 305 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATCCTTAACGAGTCAT+TGG	0.477983	2_1:+71113423	Msa0222100:CDS
AAGCTGTGGAGGACATAAAT+AGG	0.396665	2_1:+71112245	Msa0222100:CDS
AAGGAGCTGAGGTCACTTAA+TGG	0.305351	2_1:+71111924	Msa0222100:CDS
AATTGCACTAACCTCCTTTA+AGG	0.155429	2_1:+71113520	Msa0222100:three_prime_UTR
ACCAAGCTTGGAAGAACAAG+GGG	0.614422	2_1:+71111554	Msa0222100:CDS
ACCCCTTGTTCTTCCAAGCT+TGG	0.438251	2_1:-71111555	None:intergenic
ACTCTCCTTGCATATTGTGA+TGG	0.520397	2_1:-71111996	None:intergenic
ACTTATACAAATTTGAATCA+TGG	0.424889	2_1:+71113649	Msa0222100:three_prime_UTR
AGAATTGCAACTACACATTT+TGG	0.197843	2_1:+71111947	Msa0222100:CDS
AGACAATCATGACAACCTTA+AGG	0.402119	2_1:+71113494	Msa0222100:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGATGCAGAGAAGAAATT+CGG	-	chr2_1:71109281-71109300	None:intergenic	30.0%
AATTGTGAAACTTCTACCTT+TGG	-	chr2_1:71109485-71109504	None:intergenic	30.0%
AAGGTAGAAGTTTCACAATT+AGG	+	chr2_1:71109485-71109504	Msa0222100:intron	30.0%
AGATTCCAATGAGTTTAGTA+AGG	+	chr2_1:71109568-71109587	Msa0222100:intron	30.0%
AGTTCACTAACCAAAAAGTT+AGG	+	chr2_1:71109596-71109615	Msa0222100:intron	30.0%
GATACATCTATGATCTTTGA+AGG	+	chr2_1:71109897-71109916	Msa0222100:intron	30.0%
TCTTTGTAAAGCCTGTAATA+AGG	-	chr2_1:71109976-71109995	None:intergenic	30.0%
ATATAAGTTCCTTCAATTGG+TGG	-	chr2_1:71110036-71110055	None:intergenic	30.0%
CAGATATAAGTTCCTTCAAT+TGG	-	chr2_1:71110039-71110058	None:intergenic	30.0%
GAAGGAACTTATATCTGTTT+CGG	+	chr2_1:71110042-71110061	Msa0222100:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	71109162	71113700	71109162	ID=Msa0222100;Name=Msa0222100
chr2_1	mRNA	71109162	71113700	71109162	ID=Msa0222100-mRNA-1;Parent=Msa0222100;Name=Msa0222100-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0.8\|0.66\|1\|0.8\|0.66\|6\|235\|173
chr2_1	exon	71109162	71109167	71109162	ID=Msa0222100-mRNA-1:exon:20434;Parent=Msa0222100-mRNA-1
chr2_1	exon	71109934	71110077	71109934	ID=Msa0222100-mRNA-1:exon:20435;Parent=Msa0222100-mRNA-1
chr2_1	exon	71111540	71111609	71111540	ID=Msa0222100-mRNA-1:exon:20436;Parent=Msa0222100-mRNA-1
chr2_1	exon	71111916	71112082	71111916	ID=Msa0222100-mRNA-1:exon:20437;Parent=Msa0222100-mRNA-1
chr2_1	exon	71112193	71112266	71112193	ID=Msa0222100-mRNA-1:exon:20438;Parent=Msa0222100-mRNA-1
chr2_1	exon	71113405	71113700	71113405	ID=Msa0222100-mRNA-1:exon:20439;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71109162	71109167	71109162	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71109934	71110077	71109934	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71111540	71111609	71111540	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71111916	71112082	71111916	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71112193	71112266	71112193	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	CDS	71113405	71113465	71113405	ID=Msa0222100-mRNA-1:cds;Parent=Msa0222100-mRNA-1
chr2_1	three_prime_UTR	71113466	71113700	71113466	ID=Msa0222100-mRNA-1:three_prime_utr;Parent=Msa0222100-mRNA-1

Gene Sequence

>Msa0222100

ATGAATATTTTATTTATGGCAACGGCAACATACCCACCACCACCACCTTATTACAGGCTTTACAAAGATTATGTGCAAGACCCTGAGTCTGCTCCAGAGCCTCCACCACCAATTGAAGGAACTTATATCTGTTTCGGAGGCAGTTACACTACTAGTGATGTTCTACCAAGCTTGGAAGAACAAGGGGTACGCCAACTCTATTCTAAAGGACCTAATATTGATTTCAAGAAGGAGCTGAGGTCACTTAATGGAGAATTGCAACTACACATTTTGGAGCTTGCTGATATTCTTATTGAGAGACCATCACAATATGCAAGGAGAGTCGAAGAAATTTCAACTGTGTTCAAAAATTTGCATCACCTTTTGAATTCACTGCGTCCTCATCAGGCAAGAGCAACTTTGATTCACATTTTGGAACTTCAGATAGAGCGCCGTAAACAAGCTGTGGAGGACATAAATAGGAGGAGAGAAGAAGCTCGAAGAATCCTTAACGAGTCATTGGCAACACTTGATGGCCATTAA

Protein sequence

>Msa0222100

MNILFMATATYPPPPPYYRLYKDYVQDPESAPEPPPPIEGTYICFGGSYTTSDVLPSLEEQGVRQLYSKGPNIDFKKELRSLNGELQLHILELADILIERPSQYARRVEEISTVFKNLHHLLNSLRPHQARATLIHILELQIERRKQAVEDINRRREEARRILNESLATLDGH*