AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0222420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222420 MtrunA17_Chr2g0327401 97.266 512 13 1 1 512 1 511 0.0 1043
Msa0222420 MtrunA17_Chr2g0327381 71.685 558 98 6 1 509 1 547 0.0 805
Msa0222420 MtrunA17_Chr2g0327371 70.201 547 85 6 1 501 1 515 0.0 758
Msa0222420 MtrunA17_Chr1g0199171 67.083 480 147 4 39 509 20 497 0.0 672
Msa0222420 MtrunA17_Chr4g0028271 67.941 471 142 5 38 503 32 498 0.0 671
Msa0222420 MtrunA17_Chr4g0028261 68.330 461 136 6 46 500 61 517 0.0 657
Msa0222420 MtrunA17_Chr1g0171411 63.727 499 162 7 14 507 5 489 0.0 642
Msa0222420 MtrunA17_Chr4g0028281 66.013 459 149 4 46 500 38 493 0.0 642
Msa0222420 MtrunA17_Chr4g0028241 60.480 458 167 8 51 500 40 491 0.0 553
Msa0222420 MtrunA17_Chr5g0447441 32.847 411 256 8 97 499 37 435 6.78e-67 223
Msa0222420 MtrunA17_Chr4g0044051 32.360 411 258 7 97 499 41 439 2.61e-64 216
Msa0222420 MtrunA17_Chr3g0094811 30.505 436 246 17 101 503 44 455 6.48e-52 182
Msa0222420 MtrunA17_Chr3g0118851 28.571 448 261 13 79 500 74 488 6.73e-51 181
Msa0222420 MtrunA17_Chr1g0153881 30.166 421 250 11 98 500 101 495 9.17e-51 181
Msa0222420 MtrunA17_Chr8g0338461 28.051 467 254 13 101 501 45 495 1.65e-49 177
Msa0222420 MtrunA17_Chr3g0118841 28.635 447 261 14 79 500 75 488 6.09e-49 176
Msa0222420 MtrunA17_Chr7g0234051 27.349 479 263 14 86 498 33 492 8.45e-49 175
Msa0222420 MtrunA17_Chr3g0118881 29.515 454 263 14 79 503 74 499 2.19e-47 171
Msa0222420 MtrunA17_Chr3g0131731 27.790 457 279 16 72 499 24 458 7.30e-47 169
Msa0222420 MtrunA17_Chr2g0318911 28.899 436 251 13 101 503 55 464 8.30e-47 169
Msa0222420 MtrunA17_Chr7g0221551 29.466 431 248 18 103 499 46 454 8.55e-47 169
Msa0222420 MtrunA17_Chr2g0309541 29.157 439 248 15 101 503 53 464 8.85e-47 169
Msa0222420 MtrunA17_Chr3g0090331 28.947 418 264 15 98 498 85 486 9.89e-47 169
Msa0222420 MtrunA17_Chr2g0285351 27.174 460 280 12 77 501 30 469 3.41e-46 167
Msa0222420 MtrunA17_Chr2g0304931 29.091 440 249 18 96 499 47 459 4.66e-46 167
Msa0222420 MtrunA17_Chr2g0309521 28.972 428 261 13 101 503 54 463 5.18e-46 167
Msa0222420 MtrunA17_Chr1g0153941 28.018 439 257 12 101 500 92 510 3.10e-45 166
Msa0222420 MtrunA17_Chr8g0385871 27.610 431 262 17 101 499 47 459 6.13e-45 164
Msa0222420 MtrunA17_Chr2g0309511 28.878 419 273 12 101 503 54 463 7.31e-45 164
Msa0222420 MtrunA17_Chr4g0020241 28.009 432 258 14 93 498 79 483 8.23e-45 164
Msa0222420 MtrunA17_Chr4g0068841 26.697 442 273 14 99 503 51 478 2.32e-44 163
Msa0222420 MtrunA17_Chr3g0118831 29.803 406 241 12 101 501 92 458 4.33e-44 162
Msa0222420 MtrunA17_Chr5g0431301 28.222 450 290 14 78 501 18 460 5.29e-44 161
Msa0222420 MtrunA17_Chr4g0047861 26.411 443 271 12 93 499 59 482 1.01e-43 161
Msa0222420 MtrunA17_Chr2g0285371 25.977 435 271 12 103 501 50 469 2.89e-43 159
Msa0222420 MtrunA17_Chr4g0038311 28.169 426 266 12 101 499 48 460 5.65e-43 159
Msa0222420 MtrunA17_Chr4g0009681 29.540 457 265 18 72 495 29 461 1.23e-42 158
Msa0222420 MtrunA17_Chr7g0249431 36.782 261 141 8 101 349 42 290 1.57e-42 158
Msa0222420 MtrunA17_Chr3g0126031 27.650 434 267 14 98 501 39 455 9.61e-42 155
Msa0222420 MtrunA17_Chr4g0009711 28.744 414 246 14 115 496 69 465 2.20e-41 154
Msa0222420 MtrunA17_Chr3g0093971 27.938 451 277 12 86 501 17 454 2.63e-41 154
Msa0222420 MtrunA17_Chr2g0285381 27.660 423 268 12 103 499 53 463 3.40e-41 154
Msa0222420 MtrunA17_Chr1g0199991 26.651 424 269 14 101 499 47 453 4.28e-41 153
Msa0222420 MtrunA17_Chr7g0219051 27.231 437 252 15 103 499 51 461 2.28e-40 151
Msa0222420 MtrunA17_Chr3g0126051 27.739 429 285 12 93 501 39 462 2.98e-40 151
Msa0222420 MtrunA17_Chr2g0281621 26.037 434 266 12 101 499 49 462 3.87e-40 151
Msa0222420 MtrunA17_Chr3g0118891 27.252 444 239 13 79 500 74 455 4.58e-40 150
Msa0222420 MtrunA17_Chr4g0009651 27.729 458 274 17 72 496 32 465 9.07e-40 150
Msa0222420 MtrunA17_Chr4g0009661 26.895 409 260 11 115 496 69 465 9.73e-40 150
Msa0222420 MtrunA17_Chr4g0009701 27.830 424 262 14 103 496 56 465 1.07e-39 150
Msa0222420 MtrunA17_Chr4g0009671 27.053 414 253 13 115 496 34 430 2.42e-39 148
Msa0222420 MtrunA17_Chr3g0118861 27.674 430 228 14 101 500 90 466 2.15e-38 146
Msa0222420 MtrunA17_Chr1g0199971 25.587 426 271 12 101 499 47 453 4.62e-38 145
Msa0222420 MtrunA17_Chr3g0133011 25.397 441 293 10 86 496 57 491 6.61e-37 142
Msa0222420 MtrunA17_Chr4g0068861 28.571 385 214 13 95 436 93 459 7.02e-37 142
Msa0222420 MtrunA17_Chr7g0234041 36.296 270 133 9 86 349 30 266 1.39e-34 135
Msa0222420 MtrunA17_Chr4g0027731 27.059 425 255 12 114 497 44 454 1.86e-33 132
Msa0222420 MtrunA17_Chr4g0009641 25.124 402 252 12 115 496 69 441 6.11e-33 130
Msa0222420 MtrunA17_Chr1g0165811 35.000 220 131 6 103 319 46 256 2.89e-32 124
Msa0222420 MtrunA17_Chr1g0153931 39.053 169 98 5 101 265 90 257 6.05e-32 124
Msa0222420 MtrunA17_Chr2g0281641 24.495 396 247 10 138 499 7 384 1.40e-31 125
Msa0222420 MtrunA17_Chr2g0279371 31.868 182 100 3 74 250 19 181 2.66e-27 108
Msa0222420 MtrunA17_Chr2g0320571 26.304 460 278 14 84 492 11 460 6.44e-25 107
Msa0222420 MtrunA17_Chr1g0204191 25.114 438 230 21 103 499 107 487 2.20e-23 103
Msa0222420 MtrunA17_Chr4g0009721 23.944 426 204 16 115 496 65 414 2.25e-21 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222420 AT3G45010.1 66.267 501 159 5 12 505 8 505 0.0 691
Msa0222420 AT3G10410.1 62.992 508 165 6 5 502 4 498 0.0 677
Msa0222420 AT5G22980.1 62.450 498 176 5 13 503 5 498 0.0 644
Msa0222420 AT5G22960.1 62.874 167 42 2 95 261 28 174 1.96e-67 216
Msa0222420 AT1G15000.1 30.488 410 262 9 97 499 31 424 2.99e-54 189
Msa0222420 AT3G25420.1 28.541 466 253 13 101 501 44 494 1.37e-52 186
Msa0222420 AT3G12203.3 27.854 438 264 16 89 499 2 414 6.75e-52 182
Msa0222420 AT3G12203.1 27.460 437 267 14 89 499 23 435 1.83e-51 181
Msa0222420 AT1G73300.1 28.833 437 255 14 86 499 36 439 1.91e-51 181
Msa0222420 AT1G73300.2 28.833 437 255 14 86 499 36 439 1.91e-51 181
Msa0222420 AT2G22920.2 29.252 441 271 16 73 498 18 432 2.46e-50 178
Msa0222420 AT3G12203.2 28.607 402 244 15 116 499 1 377 2.65e-50 177
Msa0222420 AT2G22970.1 28.507 442 273 16 73 499 18 431 6.32e-50 177
Msa0222420 AT3G17180.1 28.894 443 254 15 98 499 50 472 1.15e-48 175
Msa0222420 AT1G73290.1 27.126 435 263 14 86 499 35 436 1.48e-48 174
Msa0222420 AT5G36180.1 28.345 441 254 15 85 499 35 439 1.89e-48 173
Msa0222420 AT4G12910.1 28.216 482 258 15 86 501 38 497 2.70e-48 174
Msa0222420 AT1G73280.1 27.689 437 262 15 85 499 35 439 3.53e-48 172
Msa0222420 AT5G23210.1 29.933 451 249 15 101 503 65 496 4.86e-48 174
Msa0222420 AT2G22980.1 28.315 445 267 18 73 499 18 428 7.51e-48 171
Msa0222420 AT3G52000.1 28.125 416 261 11 101 497 78 474 1.19e-47 172
Msa0222420 AT2G23010.2 27.578 446 276 15 73 499 18 435 5.49e-47 169
Msa0222420 AT1G73270.2 25.517 435 278 13 85 501 35 441 1.24e-46 168
Msa0222420 AT2G23010.1 27.578 446 276 15 73 499 18 435 1.53e-46 168
Msa0222420 AT1G73270.1 25.517 435 278 13 85 501 35 441 1.66e-46 168
Msa0222420 AT4G15100.1 28.306 431 265 14 101 498 58 477 1.74e-46 169
Msa0222420 AT3G10450.1 27.714 433 267 16 85 499 31 435 3.75e-46 167
Msa0222420 AT2G22920.1 29.703 404 245 14 73 462 18 396 6.01e-46 166
Msa0222420 AT1G73310.1 27.143 420 254 12 103 499 49 439 8.22e-46 166
Msa0222420 AT4G30810.1 28.046 435 264 13 99 504 50 464 1.05e-45 167
Msa0222420 AT3G10450.2 27.361 413 258 14 105 499 4 392 1.49e-45 164
Msa0222420 AT1G28110.1 28.037 428 260 16 101 498 45 454 1.73e-45 166
Msa0222420 AT1G28110.2 28.037 428 260 16 101 498 45 454 1.73e-45 166
Msa0222420 AT3G63470.1 29.356 419 243 15 101 492 95 487 1.74e-45 167
Msa0222420 AT4G12910.2 36.059 269 150 6 86 349 38 289 1.89e-45 166
Msa0222420 AT2G22970.3 28.810 420 257 15 73 477 18 410 2.23e-45 165
Msa0222420 AT2G22970.4 28.640 419 257 15 73 476 18 409 2.61e-45 164
Msa0222420 AT2G33530.1 28.474 439 248 18 101 499 47 459 3.95e-45 165
Msa0222420 AT1G33540.1 27.231 437 259 15 103 499 45 462 6.38e-45 164
Msa0222420 AT1G73290.3 28.610 374 220 11 86 439 35 381 6.97e-45 163
Msa0222420 AT3G52020.1 28.205 429 248 13 101 497 93 493 8.87e-45 165
Msa0222420 AT1G33540.2 27.126 435 258 15 105 499 4 419 2.03e-44 162
Msa0222420 AT5G08260.1 30.345 435 239 17 101 492 53 466 2.37e-44 163
Msa0222420 AT2G22980.4 28.182 440 264 18 73 494 18 423 2.50e-44 162
Msa0222420 AT2G22980.2 28.199 422 252 17 73 476 18 406 3.98e-44 161
Msa0222420 AT2G22990.1 26.139 417 264 12 103 499 39 431 7.74e-44 160
Msa0222420 AT2G35770.1 29.829 409 248 13 103 489 54 445 8.39e-44 161
Msa0222420 AT2G23000.1 26.923 442 266 13 90 499 19 435 4.88e-43 159
Msa0222420 AT2G23000.2 26.923 442 266 13 90 499 19 435 4.88e-43 159
Msa0222420 AT3G52010.1 27.934 426 268 12 92 497 73 479 4.91e-43 159
Msa0222420 AT1G61130.1 28.176 433 255 15 101 498 45 456 5.94e-43 159
Msa0222420 AT1G11080.1 26.984 441 260 12 101 498 64 485 1.55e-42 158
Msa0222420 AT3G12240.1 27.336 428 245 17 102 499 43 434 1.60e-42 157
Msa0222420 AT1G11080.2 26.984 441 260 12 101 498 64 485 1.64e-42 158
Msa0222420 AT3G12220.1 26.168 428 282 14 86 501 30 435 2.40e-42 157
Msa0222420 AT5G23210.3 30.790 367 196 12 101 421 65 419 2.81e-42 157
Msa0222420 AT2G24000.1 27.506 429 261 16 103 498 46 457 4.77e-42 156
Msa0222420 AT3G12230.1 27.295 414 263 12 102 499 42 433 8.49e-42 155
Msa0222420 AT3G12220.2 26.065 399 265 12 115 501 25 405 9.74e-42 154
Msa0222420 AT3G10450.4 27.561 410 252 15 85 476 31 413 1.20e-41 154
Msa0222420 AT1G73310.2 27.154 383 229 10 103 463 49 403 1.31e-41 154
Msa0222420 AT1G43780.1 28.111 434 256 17 103 499 53 467 1.49e-41 155
Msa0222420 AT1G73290.2 31.985 272 160 7 86 343 35 295 1.68e-41 151
Msa0222420 AT2G24010.1 28.235 425 255 15 103 499 46 448 1.69e-41 155
Msa0222420 AT5G42230.1 28.372 430 267 16 101 501 42 459 2.98e-41 154
Msa0222420 AT5G42230.2 28.372 430 267 16 101 501 42 459 2.98e-41 154
Msa0222420 AT3G07990.1 28.436 422 270 13 98 499 45 454 3.95e-41 154
Msa0222420 AT3G10450.3 27.179 390 243 13 105 476 4 370 5.46e-41 151
Msa0222420 AT2G12480.3 27.857 420 272 13 103 501 47 456 8.32e-41 153
Msa0222420 AT4G30610.1 25.116 430 279 12 99 499 44 459 2.97e-40 151
Msa0222420 AT2G12480.4 27.873 409 262 12 103 501 47 432 5.11e-40 150
Msa0222420 AT2G22990.4 26.506 415 256 13 86 478 26 413 5.13e-40 150
Msa0222420 AT2G22990.3 26.521 411 255 13 86 476 26 409 7.18e-40 150
Msa0222420 AT3G17180.2 29.306 389 220 13 98 446 50 423 1.57e-39 149
Msa0222420 AT2G12480.1 26.914 405 269 11 103 501 47 430 1.83e-39 149
Msa0222420 AT5G42240.1 26.168 428 279 14 101 501 46 463 3.00e-39 149
Msa0222420 AT2G22920.4 32.803 314 181 11 73 375 18 312 3.62e-39 145
Msa0222420 AT5G09640.1 25.160 469 285 17 73 499 19 463 4.40e-39 148
Msa0222420 AT2G12480.2 26.601 406 270 11 103 501 47 431 8.80e-39 147
Msa0222420 AT2G22970.2 32.177 317 184 11 73 378 18 314 2.78e-38 142
Msa0222420 AT5G23210.5 29.298 413 226 14 138 503 8 401 8.59e-38 143
Msa0222420 AT1G73280.2 34.855 241 137 5 85 309 35 271 8.62e-38 143
Msa0222420 AT5G23210.2 29.126 412 226 14 139 503 8 400 4.31e-37 141
Msa0222420 AT2G22990.5 24.700 417 261 13 103 499 39 422 4.41e-37 142
Msa0222420 AT3G02110.1 26.651 439 257 14 103 499 53 468 5.31e-37 142
Msa0222420 AT2G24010.2 27.273 440 255 17 103 499 46 463 7.08e-37 142
Msa0222420 AT2G35780.1 25.701 428 270 11 99 499 42 448 8.78e-37 142
Msa0222420 AT2G05850.1 26.376 436 287 15 78 497 62 479 1.43e-36 142
Msa0222420 AT2G22980.3 31.329 316 181 11 73 375 18 310 1.48e-36 139
Msa0222420 AT2G24010.3 26.797 459 258 19 103 499 17 459 1.57e-33 132
Msa0222420 AT3G12220.3 33.010 206 131 4 102 301 42 246 2.10e-32 128
Msa0222420 AT3G56540.1 42.553 141 78 3 101 239 93 232 1.41e-31 123
Msa0222420 AT5G23210.4 29.878 328 173 11 139 421 8 323 2.27e-31 125
Msa0222420 AT1G73280.4 25.424 354 219 11 163 499 12 337 2.39e-31 124
Msa0222420 AT5G36180.5 26.257 358 211 11 163 499 12 337 3.87e-31 123
Msa0222420 AT5G36180.3 26.257 358 211 11 163 499 12 337 3.87e-31 123
Msa0222420 AT2G24010.4 34.101 217 121 7 103 299 17 231 5.21e-28 115
Msa0222420 AT2G22990.2 23.824 340 220 10 175 499 2 317 1.37e-27 113
Msa0222420 AT1G73280.3 24.412 340 212 11 177 499 1 312 2.69e-27 112
Msa0222420 AT2G22990.6 23.824 340 220 10 175 499 2 317 3.55e-27 112
Msa0222420 AT5G36180.2 25.970 335 196 10 163 476 12 315 4.43e-27 112
Msa0222420 AT2G27920.1 25.118 422 259 14 114 492 45 452 6.15e-27 114
Msa0222420 AT5G36180.4 27.273 319 179 10 163 460 12 298 5.46e-26 108
Msa0222420 AT1G73310.3 24.843 318 194 8 163 463 12 301 5.95e-25 105
Msa0222420 AT2G22980.5 24.841 314 198 10 175 476 2 289 7.05e-25 105
Msa0222420 AT2G27920.2 24.416 385 240 13 145 492 10 380 1.20e-20 94.4
Msa0222420 AT2G27920.3 23.478 345 221 11 184 492 48 385 1.81e-15 78.6
Msa0222420 AT2G22920.3 21.481 270 183 9 239 498 3 253 2.24e-11 64.7

Find 122 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCTCTGGTGTGTTTA+TGG 0.125077 2_1:+71671997 None:intergenic
ACAACATGGCATCATCTTTA+AGG 0.142828 2_1:-71672192 Msa0222420:exon
TCATATTGTATACACATTAA+TGG 0.222805 2_1:-71668496 Msa0222420:three_prime_UTR
TATGCCTTGTACTGAATTTC+CGG 0.228537 2_1:+71669984 None:intergenic
ATACAATATGAATGTGATTT+TGG 0.237342 2_1:+71668508 None:intergenic
TCCCTTGTTTGGAATGAATA+TGG 0.250663 2_1:-71671144 Msa0222420:CDS
CACAAGATTCTATAGCCTTT+TGG 0.276751 2_1:+71669886 None:intergenic
AGCTTTAGGTGTTGGGGACT+TGG 0.302455 2_1:-71669464 Msa0222420:CDS
TTATCCTTGGAGAATTTAAA+AGG 0.303425 2_1:+71671174 None:intergenic
AGGATGACCCTGTTGTTATA+TGG 0.306709 2_1:-71671251 Msa0222420:CDS
ATAAGAACTTGTCCCTTGTT+TGG 0.312379 2_1:-71671155 Msa0222420:CDS
ATATCATACATTAATGTTGC+CGG 0.346161 2_1:+71669669 None:intergenic
AGACAATGGACTGATTAATA+AGG 0.347573 2_1:-71669946 Msa0222420:CDS
CCTGTTGTTATATGGTTAAC+TGG 0.347839 2_1:-71671243 Msa0222420:CDS
AACATCTGAAGTGCAGCTTT+TGG 0.363126 2_1:+71668611 None:intergenic
GTCTCCTACAACCCCGTATC+TGG 0.372478 2_1:-71668984 Msa0222420:CDS
CACTTCAGATGTTAAAAGAT+TGG 0.373515 2_1:-71668601 Msa0222420:CDS
TAAGGGAAGCTTTAGGTGTT+GGG 0.381875 2_1:-71669471 Msa0222420:CDS
CCATTGTCTAATGCAAAATC+TGG 0.382981 2_1:+71669960 None:intergenic
TGATCTATATGATTTCTTGC+AGG 0.391482 2_1:-71670481 Msa0222420:intron
CCAGTTAACCATATAACAAC+AGG 0.402521 2_1:+71671243 None:intergenic
AGTATACAGTGCCATGTTGC+AGG 0.410064 2_1:-71669419 Msa0222420:CDS
TTACAAAAGACAGACACATT+TGG 0.417560 2_1:+71672164 None:intergenic
GAGGAGAATGTCATTGTTTG+TGG 0.420601 2_1:+71672096 None:intergenic
AAGGGACAAGTTCTTATCCT+TGG 0.432855 2_1:+71671161 None:intergenic
GTAGACCAACCCACTGGAAC+AGG 0.438401 2_1:-71670561 Msa0222420:CDS
GGCTGGTCACATGGTTCCTA+TGG 0.441603 2_1:-71668639 Msa0222420:CDS
TTTGATTTGTTAGGCACTAA+AGG 0.444714 2_1:-71669757 Msa0222420:intron
GGTGTGTTTATGGAATCTTT+TGG 0.445106 2_1:+71672007 None:intergenic
CTTTGAATCTCGAAACACTA+AGG 0.445707 2_1:-71671271 Msa0222420:CDS
CTCATTTGGCAGGGAATTCG+AGG 0.446175 2_1:-71669051 Msa0222420:intron
AAGAAGATCTCATATGCAAT+TGG 0.456279 2_1:-71669321 Msa0222420:CDS
GAAAAGAAGTTCACTTTCCC+TGG 0.457022 2_1:-71671947 Msa0222420:CDS
TTGTGAATTTAAGAAATAGT+AGG 0.458804 2_1:+71668393 None:intergenic
TCTTGAACATGAAGTCAGTA+AGG 0.459917 2_1:-71669489 Msa0222420:CDS
ATCAAGGTGCTTGTGTATGC+TGG 0.464364 2_1:-71669346 Msa0222420:CDS
CTTCACTATCAACCAGATAC+GGG 0.465787 2_1:+71668972 None:intergenic
TACCTTGCAGCTTGGGAATG+AGG 0.467604 2_1:+71671863 None:intergenic
CAAGCAGAAAATCTCATAAG+AGG 0.468099 2_1:-71672043 Msa0222420:CDS
GGTGCTTCTGTTGAAGAACT+TGG 0.468257 2_1:-71671911 Msa0222420:CDS
TCATTGTTTGTGGGATAAGC+TGG 0.472247 2_1:+71672106 None:intergenic
ATTCCTGCCCTTCTTGAGGA+TGG 0.475493 2_1:-71669370 Msa0222420:CDS
TCAACCAGATACGGGGTTGT+AGG 0.477869 2_1:+71668980 None:intergenic
GTAAGGGAAGCTTTAGGTGT+TGG 0.480724 2_1:-71669472 Msa0222420:CDS
CGGACCTCTTGCTTTCCTCA+AGG 0.484984 2_1:-71668927 Msa0222420:intron
GTTCAAGAATGTTTCCGCAT+TGG 0.488329 2_1:+71669504 None:intergenic
ACAGTGCCATGTTGCAGGAT+TGG 0.491110 2_1:-71669414 Msa0222420:CDS
TCTTCACTATCAACCAGATA+CGG 0.495278 2_1:+71668971 None:intergenic
TGAAATATTTGATCAGATCA+TGG 0.497168 2_1:-71669698 Msa0222420:CDS
CCTTCTTGAGGATGGAATCA+AGG 0.500451 2_1:-71669362 Msa0222420:CDS
AAGTCAGTAAGGGAAGCTTT+AGG 0.501266 2_1:-71669478 Msa0222420:CDS
AGGAATTCATATAAATCTAA+AGG 0.501724 2_1:-71670144 Msa0222420:intron
ACAAAATGAGGATCATTCTC+TGG 0.506628 2_1:+71671986 None:intergenic
CTTGTACTGAATTTCCGGAT+TGG 0.507308 2_1:+71669989 None:intergenic
CAAGGAAACAAGGCAAAAGA+AGG 0.507437 2_1:-71670164 Msa0222420:CDS
GCAATTTCACTGCTGCATCC+TGG 0.508823 2_1:+71671216 None:intergenic
GGGTATTCCTGCCCTTCTTG+AGG 0.511069 2_1:-71669374 Msa0222420:CDS
GGATGCAAGGGAAACTGATA+AGG 0.515489 2_1:-71668580 Msa0222420:CDS
CCTTGATTCCATCCTCAAGA+AGG 0.523813 2_1:+71669362 None:intergenic
TGAACCCTAGATGCAAAAGC+TGG 0.524049 2_1:+71670188 None:intergenic
ATATGGTTAACTGGAGGGCC+AGG 0.526048 2_1:-71671234 Msa0222420:CDS
GTTGTTCCCACGATGCCAAA+AGG 0.528378 2_1:-71669901 Msa0222420:CDS
ATGTCCAGCGTATGATTCTC+CGG 0.529003 2_1:+71670217 None:intergenic
AAGATGATGCAGTTGAAGAG+AGG 0.530703 2_1:+71672077 None:intergenic
GAAGAACTTGGTCATCATGC+AGG 0.536316 2_1:-71671899 Msa0222420:CDS
GTTGATGCCATGACATGGTC+AGG 0.537146 2_1:-71669025 Msa0222420:CDS
TGAAACCTGTTCCAGTGGGT+TGG 0.537449 2_1:+71670556 None:intergenic
ATTCCATCCTCAAGAAGGGC+AGG 0.539828 2_1:+71669367 None:intergenic
ATGTTAAAAGATTGGATGCA+AGG 0.542286 2_1:-71668593 Msa0222420:CDS
AATCATTCTTAGTGAACTGA+GGG 0.546470 2_1:+71670249 None:intergenic
CAATCCGGAAATTCAGTACA+AGG 0.549343 2_1:-71669988 Msa0222420:CDS
GCACCAGAGTCAACAAAACC+AGG 0.549754 2_1:+71671929 None:intergenic
TTGTTTGGAATGAATATGGA+TGG 0.552020 2_1:-71671140 Msa0222420:CDS
AGGAGAATGTCATTGTTTGT+GGG 0.552520 2_1:+71672097 None:intergenic
TGGTAACGGATTAACCAATC+CGG 0.554595 2_1:-71670003 Msa0222420:CDS
GATCAGATCATGGACATAGC+CGG 0.558001 2_1:-71669688 Msa0222420:CDS
CTGGTTGATAGTGAAGAAGC+AGG 0.565072 2_1:-71668965 Msa0222420:CDS
GACATTAGAAAGCAATGTGT+GGG 0.565208 2_1:-71669547 Msa0222420:CDS
TTGTTATATGGTTAACTGGA+GGG 0.566502 2_1:-71671239 Msa0222420:CDS
TTTGCATCTAGGGTTCATCA+AGG 0.567295 2_1:-71670182 Msa0222420:CDS
GATCTCATATGCAATTGGCT+TGG 0.569300 2_1:-71669316 Msa0222420:intron
TGTTAAAAGATTGGATGCAA+GGG 0.569319 2_1:-71668592 Msa0222420:CDS
ATATTTCCATGAACAAAATG+AGG 0.569648 2_1:+71671974 None:intergenic
AAGGGAAGCTTTAGGTGTTG+GGG 0.573810 2_1:-71669470 Msa0222420:CDS
GTTGTTATATGGTTAACTGG+AGG 0.581708 2_1:-71671240 Msa0222420:CDS
CAAAAGACAGACACATTTGG+AGG 0.583029 2_1:+71672167 None:intergenic
TTTGGCAGGGAATTCGAGGT+GGG 0.585336 2_1:-71669047 Msa0222420:intron
TGGGAACAACTTGTTGATGT+TGG 0.588848 2_1:+71669914 None:intergenic
GGTTAAAGAGGCTGGTCACA+TGG 0.594849 2_1:-71668648 Msa0222420:CDS
ATTTGGCAGGGAATTCGAGG+TGG 0.595991 2_1:-71669048 Msa0222420:intron
AGAATGAATGTGAAATGAAG+AGG 0.598446 2_1:+71672134 None:intergenic
CTTGAACATGAAGTCAGTAA+GGG 0.603412 2_1:-71669488 Msa0222420:CDS
ATCCATATTCATTCCAAACA+AGG 0.604369 2_1:+71671142 None:intergenic
TTCCTCATTCCCAAGCTGCA+AGG 0.607253 2_1:-71671865 Msa0222420:intron
TTTCATCCAATCCTGCAACA+TGG 0.613087 2_1:+71669408 None:intergenic
CTTGATTCCATCCTCAAGAA+GGG 0.613437 2_1:+71669363 None:intergenic
TGACATTAGAAAGCAATGTG+TGG 0.619542 2_1:-71669548 Msa0222420:CDS
GATTTGTTAGGCACTAAAGG+TGG 0.621116 2_1:-71669754 Msa0222420:intron
AAATCATTCTTAGTGAACTG+AGG 0.624376 2_1:+71670248 None:intergenic
CAAAAGGCTATAGAATCTTG+TGG 0.625610 2_1:-71669885 Msa0222420:intron
ATATTTGTAGACCAACCCAC+TGG 0.627039 2_1:-71670567 Msa0222420:CDS
TAGCTGAAACCTGTTCCAGT+GGG 0.633323 2_1:+71670552 None:intergenic
ATAGCTGAAACCTGTTCCAG+TGG 0.637939 2_1:+71670551 None:intergenic
TCCATATTCATTCCAAACAA+GGG 0.638155 2_1:+71671143 None:intergenic
GCAGGAGTTCTGAAAAGCCA+CGG 0.638418 2_1:-71668947 Msa0222420:CDS
TTCACTATCAACCAGATACG+GGG 0.643964 2_1:+71668973 None:intergenic
TCGAACGTCGCTATCATCAG+TGG 0.646032 2_1:+71670527 None:intergenic
TAGGGTTCATCAAGGAAACA+AGG 0.646926 2_1:-71670174 Msa0222420:CDS
AAGGGAAACTGATAAGGACA+AGG 0.660416 2_1:-71668574 Msa0222420:CDS
CACCAGAGTCAACAAAACCA+GGG 0.660712 2_1:+71671930 None:intergenic
AAACTGATAAGGACAAGGGG+AGG 0.667086 2_1:-71668569 Msa0222420:CDS
AGGGAAACTGATAAGGACAA+GGG 0.667941 2_1:-71668573 Msa0222420:CDS
GGTGGGTTGATGCCATGACA+TGG 0.669211 2_1:-71669030 Msa0222420:CDS
TGAGGAAAGCAAGAGGTCCG+TGG 0.669417 2_1:+71668930 None:intergenic
TTGTTATGACTTCTCCAATG+CGG 0.671699 2_1:-71669518 Msa0222420:CDS
TGTTTGGAATGAATATGGAT+GGG 0.672561 2_1:-71671139 Msa0222420:CDS
GAATGAATATGGATGGGACA+TGG 0.683982 2_1:-71671133 Msa0222420:intron
ATTACCGGAGAATCATACGC+TGG 0.686475 2_1:-71670221 Msa0222420:CDS
GGGAAACTGATAAGGACAAG+GGG 0.694048 2_1:-71668572 Msa0222420:CDS
CAAACCTTGAGGAAAGCAAG+AGG 0.699262 2_1:+71668923 None:intergenic
GACGTTCGACATGATGAAGA+CGG 0.699400 2_1:-71670513 Msa0222420:CDS
CAGTTAACCATATAACAACA+GGG 0.704037 2_1:+71671244 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTCTATTTTATCTTATTT+TGG + chr2_1:71668784-71668803 None:intergenic 15.0%
!! ATAAAATAGAATGAAATATG+AGG - chr2_1:71668791-71668810 Msa0222420:intron 15.0%
!! GCAAAGAATTTATAAAAAAA+TGG - chr2_1:71668929-71668948 Msa0222420:CDS 15.0%
!! TATAAAAAAATGGAATAGTT+TGG - chr2_1:71668939-71668958 Msa0222420:CDS 15.0%
!!! TTTGATCTAGTTTAATTTAT+TGG - chr2_1:71668976-71668995 Msa0222420:CDS 15.0%
!!! AAAGTGTTTTTCTTAAAATT+CGG + chr2_1:71669146-71669165 None:intergenic 15.0%
!!! CTCTATTGATTTATATTAAT+TGG - chr2_1:71669206-71669225 Msa0222420:intron 15.0%
!! AGAAAAGAGAATAATTTAAT+AGG + chr2_1:71669485-71669504 None:intergenic 15.0%
!!! TTAGCTATTTTTACAATAAA+AGG + chr2_1:71669740-71669759 None:intergenic 15.0%
!!! AAGAATGATTTTTATATTAC+CGG - chr2_1:71670354-71670373 Msa0222420:intron 15.0%
!! GATTATAAGTTTGATTAATT+TGG - chr2_1:71671452-71671471 Msa0222420:intron 15.0%
!! TCTTTAACCTATTAAAAAAA+CGG + chr2_1:71671931-71671950 None:intergenic 15.0%
!! CAGAATAAAAATATAGTTCT+TGG - chr2_1:71668867-71668886 Msa0222420:intron 20.0%
!!! TTGTCATCAAATTCTTATTT+TGG + chr2_1:71669186-71669205 None:intergenic 20.0%
!!! ATTGCTATGTTTTATGAAAA+TGG - chr2_1:71669392-71669411 Msa0222420:CDS 20.0%
!! TTTCATAAGCTATATTAGTT+TGG + chr2_1:71669700-71669719 None:intergenic 20.0%
!!! TTAAGTTGTTTATCCAAATA+TGG - chr2_1:71669805-71669824 Msa0222420:intron 20.0%
!! AATTATTCACTTGTATTAAG+TGG - chr2_1:71670203-71670222 Msa0222420:CDS 20.0%
!! AGGAATTCATATAAATCTAA+AGG - chr2_1:71670446-71670465 Msa0222420:intron 20.0%
!! TGTACACTATATTTATGTAT+AGG - chr2_1:71670490-71670509 Msa0222420:CDS 20.0%
!! TGTAAACCTTTATAATAAAC+AGG - chr2_1:71670554-71670573 Msa0222420:CDS 20.0%
!! GTAAACCTTTATAATAAACA+GGG - chr2_1:71670555-71670574 Msa0222420:CDS 20.0%
!! AAGGAAAAATAACAAGTAAA+TGG + chr2_1:71670793-71670812 None:intergenic 20.0%
!!! TAGTTTGTATTTGATTTGTT+AGG - chr2_1:71670824-71670843 Msa0222420:intron 20.0%
!! GTATGATATCAAAAAAGAAA+AGG - chr2_1:71670935-71670954 Msa0222420:intron 20.0%
!! CAAAATAGAATACACAAATA+TGG - chr2_1:71671493-71671512 Msa0222420:intron 20.0%
!! TTATAAATGTGATTCTCATT+TGG - chr2_1:71671525-71671544 Msa0222420:intron 20.0%
!!! TTAACTTCCGTTTTTTTAAT+AGG - chr2_1:71671921-71671940 Msa0222420:CDS 20.0%
!!! GTTTTTTTAATAGGTTAAAG+AGG - chr2_1:71671930-71671949 Msa0222420:CDS 20.0%
!!! ATACAATATGAATGTGATTT+TGG + chr2_1:71672085-71672104 None:intergenic 20.0%
!! TCATATTGTATACACATTAA+TGG - chr2_1:71672094-71672113 Msa0222420:CDS 20.0%
!! TTGTGAATTTAAGAAATAGT+AGG + chr2_1:71672200-71672219 None:intergenic 20.0%
!! GATTTTCTGCTTGTAATTTT+GGG + chr2_1:71668540-71668559 None:intergenic 25.0%
!! AGATTTTCTGCTTGTAATTT+TGG + chr2_1:71668541-71668560 None:intergenic 25.0%
! ATATTTCCATGAACAAAATG+AGG + chr2_1:71668619-71668638 None:intergenic 25.0%
!! TTTTGTTCATGGAAATATTG+TGG - chr2_1:71668621-71668640 Msa0222420:CDS 25.0%
!! CATGACTATCTTTGAATTTT+AGG - chr2_1:71668758-71668777 Msa0222420:intron 25.0%
!!! AATATGAGGTAAGTTTTTTG+TGG - chr2_1:71668805-71668824 Msa0222420:intron 25.0%
!!! TTGGAGTTTAATTTTAGAAG+TGG + chr2_1:71669117-71669136 None:intergenic 25.0%
!! TCTTAAAATTCGGTCTATTT+TGG + chr2_1:71669136-71669155 None:intergenic 25.0%
! TTATCCTTGGAGAATTTAAA+AGG + chr2_1:71669419-71669438 None:intergenic 25.0%
! TATATGAGTAAGAACTAAAG+TGG + chr2_1:71669518-71669537 None:intergenic 25.0%
!!! AGACTGTTTGAGAATTTTTT+TGG - chr2_1:71669598-71669617 Msa0222420:intron 25.0%
! ACAACTTATGTCATATTTGT+AGG - chr2_1:71669623-71669642 Msa0222420:intron 25.0%
!!! TATTTGTAGGTTTTTTTGTG+AGG - chr2_1:71669636-71669655 Msa0222420:intron 25.0%
! ACATAAGCATTTATATGACA+AGG - chr2_1:71669761-71669780 Msa0222420:intron 25.0%
! CATAAGCATTTATATGACAA+GGG - chr2_1:71669762-71669781 Msa0222420:intron 25.0%
! ACAAACAAGAAAAAATGCAT+TGG + chr2_1:71669989-71670008 None:intergenic 25.0%
!!! ATGCATTTTTTCTTGTTTGT+AGG - chr2_1:71669989-71670008 Msa0222420:CDS 25.0%
! CAAAACCCTGTTTATTATAA+AGG + chr2_1:71670563-71670582 None:intergenic 25.0%
! ACAAACTATTAGTGTCAATA+AGG + chr2_1:71670812-71670831 None:intergenic 25.0%
! TGAAATATTTGATCAGATCA+TGG - chr2_1:71670892-71670911 Msa0222420:intron 25.0%
! ATATCATACATTAATGTTGC+CGG + chr2_1:71670924-71670943 None:intergenic 25.0%
!! AATTTGGAACCAAAATTTCT+TGG - chr2_1:71671468-71671487 Msa0222420:intron 25.0%
!! ATTTGGAACCAAAATTTCTT+GGG - chr2_1:71671469-71671488 Msa0222420:intron 25.0%
!!! TATTTTGACCCAAGAAATTT+TGG + chr2_1:71671480-71671499 None:intergenic 25.0%
!! TCATTCTTTTAATGTTCACA+AGG + chr2_1:71672125-71672144 None:intergenic 25.0%
TTACAAAAGACAGACACATT+TGG + chr2_1:71668429-71668448 None:intergenic 30.0%
AGAATGAATGTGAAATGAAG+AGG + chr2_1:71668459-71668478 None:intergenic 30.0%
! AATGATCCTCATTTTGTTCA+TGG - chr2_1:71668610-71668629 Msa0222420:CDS 30.0%
CGACATAACAAATTGTTCAA+TGG + chr2_1:71669277-71669296 None:intergenic 30.0%
CAGTTAACCATATAACAACA+GGG + chr2_1:71669349-71669368 None:intergenic 30.0%
TTGTTATATGGTTAACTGGA+GGG - chr2_1:71669351-71669370 Msa0222420:CDS 30.0%
TCCATATTCATTCCAAACAA+GGG + chr2_1:71669450-71669469 None:intergenic 30.0%
ATCCATATTCATTCCAAACA+AGG + chr2_1:71669451-71669470 None:intergenic 30.0%
! TTGTTTGGAATGAATATGGA+TGG - chr2_1:71669450-71669469 Msa0222420:CDS 30.0%
! TGTTTGGAATGAATATGGAT+GGG - chr2_1:71669451-71669470 Msa0222420:CDS 30.0%
TCTTGCAATAATGACATGTT+TGG - chr2_1:71669544-71669563 Msa0222420:CDS 30.0%
TGATCTATATGATTTCTTGC+AGG - chr2_1:71670109-71670128 Msa0222420:intron 30.0%
TCTCTAAATTTCACAATGAC+AGG - chr2_1:71670302-71670321 Msa0222420:intron 30.0%
AATCATTCTTAGTGAACTGA+GGG + chr2_1:71670344-71670363 None:intergenic 30.0%
AAATCATTCTTAGTGAACTG+AGG + chr2_1:71670345-71670364 None:intergenic 30.0%
!! AATAAACAGGGTTTTGCTAT+TGG - chr2_1:71670567-71670586 Msa0222420:CDS 30.0%
!! AGACAATGGACTGATTAATA+AGG - chr2_1:71670644-71670663 Msa0222420:intron 30.0%
!!! TTTTAAGTTTGTTCCCTTCT+CGG - chr2_1:71670735-71670754 Msa0222420:intron 30.0%
AAGTAAATGGCACAACAAAT+TGG + chr2_1:71670780-71670799 None:intergenic 30.0%
!! TTTGATTTGTTAGGCACTAA+AGG - chr2_1:71670833-71670852 Msa0222420:intron 30.0%
TAATGTCATAGTACTGCATT+AGG + chr2_1:71671030-71671049 None:intergenic 30.0%
TTGGATGAAAAATCTAGAAG+TGG - chr2_1:71671195-71671214 Msa0222420:CDS 30.0%
TGGATGAAAAATCTAGAAGT+GGG - chr2_1:71671196-71671215 Msa0222420:CDS 30.0%
AAGAAGATCTCATATGCAAT+TGG - chr2_1:71671269-71671288 Msa0222420:CDS 30.0%
! TCTAATCAGAATTTGAAGTC+CGG - chr2_1:71671393-71671412 Msa0222420:intron 30.0%
TAATCAATGTGCTTCACTTA+AGG + chr2_1:71671437-71671456 None:intergenic 30.0%
GTCAGGTCAAAAAGAATTTA+AGG - chr2_1:71671582-71671601 Msa0222420:intron 30.0%
! CTTCAAACAAAAAGTGTGTT+TGG - chr2_1:71671745-71671764 Msa0222420:intron 30.0%
!! TGATGTTTACATGTCACTTT+CGG - chr2_1:71671842-71671861 Msa0222420:intron 30.0%
! TTTTAATAGGTTAAAGAGGC+TGG - chr2_1:71671934-71671953 Msa0222420:CDS 30.0%
CACTTCAGATGTTAAAAGAT+TGG - chr2_1:71671989-71672008 Msa0222420:CDS 30.0%
ATGTTAAAAGATTGGATGCA+AGG - chr2_1:71671997-71672016 Msa0222420:CDS 30.0%
TGTTAAAAGATTGGATGCAA+GGG - chr2_1:71671998-71672017 Msa0222420:CDS 30.0%
ACAACATGGCATCATCTTTA+AGG - chr2_1:71668398-71668417 Msa0222420:three_prime_UTR 35.0%
AGGAGAATGTCATTGTTTGT+GGG + chr2_1:71668496-71668515 None:intergenic 35.0%
CAAGCAGAAAATCTCATAAG+AGG - chr2_1:71668547-71668566 Msa0222420:CDS 35.0%
! GGTGTGTTTATGGAATCTTT+TGG + chr2_1:71668586-71668605 None:intergenic 35.0%
ATCATTCTCTGGTGTGTTTA+TGG + chr2_1:71668596-71668615 None:intergenic 35.0%
ACAAAATGAGGATCATTCTC+TGG + chr2_1:71668607-71668626 None:intergenic 35.0%
! TCTATTTTACCTTGCAGCTT+GGG + chr2_1:71668737-71668756 None:intergenic 35.0%
! ATCTATTTTACCTTGCAGCT+TGG + chr2_1:71668738-71668757 None:intergenic 35.0%
!! CAATTTGTTATGTCGATTGC+AGG - chr2_1:71669282-71669301 Msa0222420:intron 35.0%
CTTTGAATCTCGAAACACTA+AGG - chr2_1:71669319-71669338 Msa0222420:CDS 35.0%
CCAGTTAACCATATAACAAC+AGG + chr2_1:71669350-71669369 None:intergenic 35.0%
CCTGTTGTTATATGGTTAAC+TGG - chr2_1:71669347-71669366 Msa0222420:CDS 35.0%
GTTGTTATATGGTTAACTGG+AGG - chr2_1:71669350-71669369 Msa0222420:CDS 35.0%
! TGGTCCTTTTAAATTCTCCA+AGG - chr2_1:71669412-71669431 Msa0222420:CDS 35.0%
ATAAGAACTTGTCCCTTGTT+TGG - chr2_1:71669435-71669454 Msa0222420:CDS 35.0%
! TCCCTTGTTTGGAATGAATA+TGG - chr2_1:71669446-71669465 Msa0222420:CDS 35.0%
TGATTGAACATAGCACAACA+GGG + chr2_1:71669850-71669869 None:intergenic 35.0%
TTGATTGAACATAGCACAAC+AGG + chr2_1:71669851-71669870 None:intergenic 35.0%
! CTTGATTGTTTGAGTTTGTG+TGG + chr2_1:71669927-71669946 None:intergenic 35.0%
! ACAATGACAGGCATTTTTCA+AGG - chr2_1:71670314-71670333 Msa0222420:intron 35.0%
TATGCCTTGTACTGAATTTC+CGG + chr2_1:71670609-71670628 None:intergenic 35.0%
CCATTGTCTAATGCAAAATC+TGG + chr2_1:71670633-71670652 None:intergenic 35.0%
! CCAGATTTTGCATTAGACAA+TGG - chr2_1:71670630-71670649 Msa0222420:intron 35.0%
! CACAAGATTCTATAGCCTTT+TGG + chr2_1:71670707-71670726 None:intergenic 35.0%
CAAAAGGCTATAGAATCTTG+TGG - chr2_1:71670705-71670724 Msa0222420:intron 35.0%
TTTCAGTTTATGACCGAGAA+GGG + chr2_1:71670751-71670770 None:intergenic 35.0%
! TTTTCAGTTTATGACCGAGA+AGG + chr2_1:71670752-71670771 None:intergenic 35.0%
TGACATTAGAAAGCAATGTG+TGG - chr2_1:71671042-71671061 Msa0222420:intron 35.0%
GACATTAGAAAGCAATGTGT+GGG - chr2_1:71671043-71671062 Msa0222420:intron 35.0%
TTGTTATGACTTCTCCAATG+CGG - chr2_1:71671072-71671091 Msa0222420:intron 35.0%
TCTTGAACATGAAGTCAGTA+AGG - chr2_1:71671101-71671120 Msa0222420:intron 35.0%
CTTGAACATGAAGTCAGTAA+GGG - chr2_1:71671102-71671121 Msa0222420:intron 35.0%
AGTAATATGTGTTAGTCCTC+CGG - chr2_1:71671310-71671329 Msa0222420:intron 35.0%
GACGAATTATTTAAGAGAGC+CGG + chr2_1:71671415-71671434 None:intergenic 35.0%
AAATGTGATTCTCATTTGGC+AGG - chr2_1:71671529-71671548 Msa0222420:intron 35.0%
AATGTGATTCTCATTTGGCA+GGG - chr2_1:71671530-71671549 Msa0222420:intron 35.0%
TCTTCACTATCAACCAGATA+CGG + chr2_1:71671622-71671641 None:intergenic 35.0%
TTCCTCAAGGTTTGTGTTTA+AGG - chr2_1:71671676-71671695 Msa0222420:intron 35.0%
ATCCTTAAACACAAACCTTG+AGG + chr2_1:71671681-71671700 None:intergenic 35.0%
CAAAAGACAGACACATTTGG+AGG + chr2_1:71668426-71668445 None:intergenic 40.0%
TCATTGTTTGTGGGATAAGC+TGG + chr2_1:71668487-71668506 None:intergenic 40.0%
GAGGAGAATGTCATTGTTTG+TGG + chr2_1:71668497-71668516 None:intergenic 40.0%
AAGATGATGCAGTTGAAGAG+AGG + chr2_1:71668516-71668535 None:intergenic 40.0%
GAAAAGAAGTTCACTTTCCC+TGG - chr2_1:71668643-71668662 Msa0222420:CDS 40.0%
AGGATGACCCTGTTGTTATA+TGG - chr2_1:71669339-71669358 Msa0222420:CDS 40.0%
AAGGGACAAGTTCTTATCCT+TGG + chr2_1:71669432-71669451 None:intergenic 40.0%
GAATGAATATGGATGGGACA+TGG - chr2_1:71669457-71669476 Msa0222420:CDS 40.0%
AGCACAACAGGGAAAATCTA+GGG + chr2_1:71669839-71669858 None:intergenic 40.0%
TAGCACAACAGGGAAAATCT+AGG + chr2_1:71669840-71669859 None:intergenic 40.0%
! TTGTTTGAGTTTGTGTGGAC+TGG + chr2_1:71669922-71669941 None:intergenic 40.0%
ATATTTGTAGACCAACCCAC+TGG - chr2_1:71670023-71670042 Msa0222420:intron 40.0%
! ACATTCCAGCTTTTGCATCT+AGG - chr2_1:71670397-71670416 Msa0222420:intron 40.0%
! CATTCCAGCTTTTGCATCTA+GGG - chr2_1:71670398-71670417 Msa0222420:intron 40.0%
! TTTGCATCTAGGGTTCATCA+AGG - chr2_1:71670408-71670427 Msa0222420:intron 40.0%
TAGGGTTCATCAAGGAAACA+AGG - chr2_1:71670416-71670435 Msa0222420:intron 40.0%
CAAGGAAACAAGGCAAAAGA+AGG - chr2_1:71670426-71670445 Msa0222420:intron 40.0%
!!! CAGGGTTTTGCTATTGGTAA+CGG - chr2_1:71670573-71670592 Msa0222420:CDS 40.0%
!! TGGTAACGGATTAACCAATC+CGG - chr2_1:71670587-71670606 Msa0222420:intron 40.0%
CTTGTACTGAATTTCCGGAT+TGG + chr2_1:71670604-71670623 None:intergenic 40.0%
CAATCCGGAAATTCAGTACA+AGG - chr2_1:71670602-71670621 Msa0222420:intron 40.0%
!! TGGGAACAACTTGTTGATGT+TGG + chr2_1:71670679-71670698 None:intergenic 40.0%
!! GATTTGTTAGGCACTAAAGG+TGG - chr2_1:71670836-71670855 Msa0222420:intron 40.0%
GTTCAAGAATGTTTCCGCAT+TGG + chr2_1:71671089-71671108 None:intergenic 40.0%
AAGTCAGTAAGGGAAGCTTT+AGG - chr2_1:71671112-71671131 Msa0222420:intron 40.0%
!! TAAGGGAAGCTTTAGGTGTT+GGG - chr2_1:71671119-71671138 Msa0222420:intron 40.0%
TTTCATCCAATCCTGCAACA+TGG + chr2_1:71671185-71671204 None:intergenic 40.0%
CTTGATTCCATCCTCAAGAA+GGG + chr2_1:71671230-71671249 None:intergenic 40.0%
GATCTCATATGCAATTGGCT+TGG - chr2_1:71671274-71671293 Msa0222420:CDS 40.0%
! TTTTTGACCTGACCATGTCA+TGG + chr2_1:71671575-71671594 None:intergenic 40.0%
TTCACTATCAACCAGATACG+GGG + chr2_1:71671620-71671639 None:intergenic 40.0%
CTTCACTATCAACCAGATAC+GGG + chr2_1:71671621-71671640 None:intergenic 40.0%
!! AACATCTGAAGTGCAGCTTT+TGG + chr2_1:71671982-71672001 None:intergenic 40.0%
AAGGGAAACTGATAAGGACA+AGG - chr2_1:71672016-71672035 Msa0222420:CDS 40.0%
AGGGAAACTGATAAGGACAA+GGG - chr2_1:71672017-71672036 Msa0222420:CDS 40.0%
CCAAAAATGTGAGTGACTTG+GGG + chr2_1:71672167-71672186 None:intergenic 40.0%
GCCAAAAATGTGAGTGACTT+GGG + chr2_1:71672168-71672187 None:intergenic 40.0%
TGCCAAAAATGTGAGTGACT+TGG + chr2_1:71672169-71672188 None:intergenic 40.0%
!!! TTCCCTGGTTTTGTTGACTC+TGG - chr2_1:71668658-71668677 Msa0222420:intron 45.0%
CACCAGAGTCAACAAAACCA+GGG + chr2_1:71668663-71668682 None:intergenic 45.0%
GGTGCTTCTGTTGAAGAACT+TGG - chr2_1:71668679-71668698 Msa0222420:intron 45.0%
GAAGAACTTGGTCATCATGC+AGG - chr2_1:71668691-71668710 Msa0222420:intron 45.0%
TAGGGGGTTTAGGCCATATT+TGG + chr2_1:71669821-71669840 None:intergenic 45.0%
AGGGAAAATCTAGGGGGTTT+AGG + chr2_1:71669831-71669850 None:intergenic 45.0%
CACAACAGGGAAAATCTAGG+GGG + chr2_1:71669837-71669856 None:intergenic 45.0%
GCACAACAGGGAAAATCTAG+GGG + chr2_1:71669838-71669857 None:intergenic 45.0%
TAGCTGAAACCTGTTCCAGT+GGG + chr2_1:71670041-71670060 None:intergenic 45.0%
ATAGCTGAAACCTGTTCCAG+TGG + chr2_1:71670042-71670061 None:intergenic 45.0%
GACGTTCGACATGATGAAGA+CGG - chr2_1:71670077-71670096 Msa0222420:intron 45.0%
ATTACCGGAGAATCATACGC+TGG - chr2_1:71670369-71670388 Msa0222420:intron 45.0%
ATGTCCAGCGTATGATTCTC+CGG + chr2_1:71670376-71670395 None:intergenic 45.0%
TGAACCCTAGATGCAAAAGC+TGG + chr2_1:71670405-71670424 None:intergenic 45.0%
! CTATAGCCTTTTGGCATCGT+GGG + chr2_1:71670698-71670717 None:intergenic 45.0%
! TCTATAGCCTTTTGGCATCG+TGG + chr2_1:71670699-71670718 None:intergenic 45.0%
GATCAGATCATGGACATAGC+CGG - chr2_1:71670902-71670921 Msa0222420:intron 45.0%
!! GTAAGGGAAGCTTTAGGTGT+TGG - chr2_1:71671118-71671137 Msa0222420:intron 45.0%
!! AAGGGAAGCTTTAGGTGTTG+GGG - chr2_1:71671120-71671139 Msa0222420:intron 45.0%
AGTATACAGTGCCATGTTGC+AGG - chr2_1:71671171-71671190 Msa0222420:CDS 45.0%
CCTTGATTCCATCCTCAAGA+AGG + chr2_1:71671231-71671250 None:intergenic 45.0%
CCTTCTTGAGGATGGAATCA+AGG - chr2_1:71671228-71671247 Msa0222420:CDS 45.0%
! ATCAAGGTGCTTGTGTATGC+TGG - chr2_1:71671244-71671263 Msa0222420:CDS 45.0%
!! CTGGTTGATAGTGAAGAAGC+AGG - chr2_1:71671625-71671644 Msa0222420:intron 45.0%
CAAACCTTGAGGAAAGCAAG+AGG + chr2_1:71671670-71671689 None:intergenic 45.0%
!! TCACTTTCGGTGTTGTGTCT+GGG - chr2_1:71671855-71671874 Msa0222420:intron 45.0%
!! GCAGCTTTTGGTTGATCCAT+AGG + chr2_1:71671970-71671989 None:intergenic 45.0%
GGATGCAAGGGAAACTGATA+AGG - chr2_1:71672010-71672029 Msa0222420:CDS 45.0%
GGGAAACTGATAAGGACAAG+GGG - chr2_1:71672018-71672037 Msa0222420:CDS 45.0%
AAACTGATAAGGACAAGGGG+AGG - chr2_1:71672021-71672040 Msa0222420:CDS 45.0%
! CCCCAAGTCACTCACATTTT+TGG - chr2_1:71672164-71672183 Msa0222420:CDS 45.0%
GCACCAGAGTCAACAAAACC+AGG + chr2_1:71668664-71668683 None:intergenic 50.0%
TTCCTCATTCCCAAGCTGCA+AGG - chr2_1:71668725-71668744 Msa0222420:intron 50.0%
TACCTTGCAGCTTGGGAATG+AGG + chr2_1:71668730-71668749 None:intergenic 50.0%
ATATGGTTAACTGGAGGGCC+AGG - chr2_1:71669356-71669375 Msa0222420:CDS 50.0%
GCAATTTCACTGCTGCATCC+TGG + chr2_1:71669377-71669396 None:intergenic 50.0%
TGAAACCTGTTCCAGTGGGT+TGG + chr2_1:71670037-71670056 None:intergenic 50.0%
TCGAACGTCGCTATCATCAG+TGG + chr2_1:71670066-71670085 None:intergenic 50.0%
GTTGTTCCCACGATGCCAAA+AGG - chr2_1:71670689-71670708 Msa0222420:intron 50.0%
!! AGCTTTAGGTGTTGGGGACT+TGG - chr2_1:71671126-71671145 Msa0222420:intron 50.0%
ACAGTGCCATGTTGCAGGAT+TGG - chr2_1:71671176-71671195 Msa0222420:CDS 50.0%
ATTCCTGCCCTTCTTGAGGA+TGG - chr2_1:71671220-71671239 Msa0222420:CDS 50.0%
ATTCCATCCTCAAGAAGGGC+AGG + chr2_1:71671226-71671245 None:intergenic 50.0%
TGTTAGTCCTCCGGTTTCCA+AGG - chr2_1:71671319-71671338 Msa0222420:intron 50.0%
TTACCCGGTTCCTTGGAAAC+CGG + chr2_1:71671332-71671351 None:intergenic 50.0%
CTTACTTTCCAGCCAAACGC+TGG + chr2_1:71671356-71671375 None:intergenic 50.0%
CTCATTTGGCAGGGAATTCG+AGG - chr2_1:71671539-71671558 Msa0222420:intron 50.0%
ATTTGGCAGGGAATTCGAGG+TGG - chr2_1:71671542-71671561 Msa0222420:intron 50.0%
TTTGGCAGGGAATTCGAGGT+GGG - chr2_1:71671543-71671562 Msa0222420:intron 50.0%
GTTGATGCCATGACATGGTC+AGG - chr2_1:71671565-71671584 Msa0222420:intron 50.0%
TCAACCAGATACGGGGTTGT+AGG + chr2_1:71671613-71671632 None:intergenic 50.0%
!! GCAGGAGTTCTGAAAAGCCA+CGG - chr2_1:71671643-71671662 Msa0222420:intron 50.0%
!! GTCACTTTCGGTGTTGTGTC+TGG - chr2_1:71671854-71671873 Msa0222420:intron 50.0%
! TGTCTGGGACAAGTGTCTGT+CGG - chr2_1:71671870-71671889 Msa0222420:CDS 50.0%
! GGTTAAAGAGGCTGGTCACA+TGG - chr2_1:71671942-71671961 Msa0222420:CDS 50.0%
GTAGACCAACCCACTGGAAC+AGG - chr2_1:71670029-71670048 Msa0222420:intron 55.0%
GGGTATTCCTGCCCTTCTTG+AGG - chr2_1:71671216-71671235 Msa0222420:CDS 55.0%
TCCTCCGGTTTCCAAGGAAC+CGG - chr2_1:71671325-71671344 Msa0222420:intron 55.0%
GAACCGGGTAATCCAGCGTT+TGG - chr2_1:71671341-71671360 Msa0222420:intron 55.0%
CAGCCAAACGCTGGATTACC+CGG + chr2_1:71671347-71671366 None:intergenic 55.0%
!! GGTGGGTTGATGCCATGACA+TGG - chr2_1:71671560-71671579 Msa0222420:intron 55.0%
GTCTCCTACAACCCCGTATC+TGG - chr2_1:71671606-71671625 Msa0222420:intron 55.0%
TGAGGAAAGCAAGAGGTCCG+TGG + chr2_1:71671663-71671682 None:intergenic 55.0%
CGGACCTCTTGCTTTCCTCA+AGG - chr2_1:71671663-71671682 Msa0222420:intron 55.0%
! GGCTGGTCACATGGTTCCTA+TGG - chr2_1:71671951-71671970 Msa0222420:CDS 55.0%
CCTCCGGTTTCCAAGGAACC+GGG - chr2_1:71671326-71671345 Msa0222420:intron 60.0%
CGCTGGATTACCCGGTTCCT+TGG + chr2_1:71671339-71671358 None:intergenic 60.0%
CGGGTAATCCAGCGTTTGGC+TGG - chr2_1:71671345-71671364 Msa0222420:intron 60.0%
CCCGGTTCCTTGGAAACCGG+AGG + chr2_1:71671329-71671348 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 71668386 71672226 71668386 ID=Msa0222420;Name=Msa0222420
chr2_1 mRNA 71668386 71672226 71668386 ID=Msa0222420-mRNA-1;Parent=Msa0222420;Name=Msa0222420-mRNA-1;_AED=0.35;_eAED=0.35;_QI=17|1|1|1|1|1|9|161|512
chr2_1 exon 71668386 71668669 71668386 ID=Msa0222420-mRNA-1:exon:20623;Parent=Msa0222420-mRNA-1
chr2_1 exon 71668928 71669061 71668928 ID=Msa0222420-mRNA-1:exon:20622;Parent=Msa0222420-mRNA-1
chr2_1 exon 71669317 71669575 71669317 ID=Msa0222420-mRNA-1:exon:20621;Parent=Msa0222420-mRNA-1
chr2_1 exon 71669678 71669766 71669678 ID=Msa0222420-mRNA-1:exon:20620;Parent=Msa0222420-mRNA-1
chr2_1 exon 71669886 71670036 71669886 ID=Msa0222420-mRNA-1:exon:20619;Parent=Msa0222420-mRNA-1
chr2_1 exon 71670145 71670288 71670145 ID=Msa0222420-mRNA-1:exon:20618;Parent=Msa0222420-mRNA-1
chr2_1 exon 71670482 71670601 71670482 ID=Msa0222420-mRNA-1:exon:20617;Parent=Msa0222420-mRNA-1
chr2_1 exon 71671134 71671308 71671134 ID=Msa0222420-mRNA-1:exon:20616;Parent=Msa0222420-mRNA-1
chr2_1 exon 71671866 71672226 71671866 ID=Msa0222420-mRNA-1:exon:20615;Parent=Msa0222420-mRNA-1
chr2_1 five_prime_UTR 71672210 71672226 71672210 ID=Msa0222420-mRNA-1:five_prime_utr;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71671866 71672209 71671866 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71671134 71671308 71671134 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71670482 71670601 71670482 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71670145 71670288 71670145 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71669886 71670036 71669886 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71669678 71669766 71669678 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71669317 71669575 71669317 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71668928 71669061 71668928 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 CDS 71668547 71668669 71668547 ID=Msa0222420-mRNA-1:cds;Parent=Msa0222420-mRNA-1
chr2_1 three_prime_UTR 71668386 71668546 71668386 ID=Msa0222420-mRNA-1:three_prime_utr;Parent=Msa0222420-mRNA-1
Gene Sequence

>Msa0222420

ATGGCATCATCTTTAAGGTTCCTCCAAATGTGTCTGTCTTTTGTAATGTTCTTCCTCTTCATTTCACATTCATTCTCATCTCCAGCTTATCCCACAAACAATGACATTCTCCTCTCTTCAACTGCATCATCTTACCCAAAATTACAAGCAGAAAATCTCATAAGAGGACTTAATCTTTTTCCAAAAGATTCCATAAACACACCAGAGAATGATCCTCATTTTGTTCATGGAAATATTGTGGAAAAGAAGTTCACTTTCCCTGGTTTTGTTGACTCTGGTGCTTCTGTTGAAGAACTTGGTCATCATGCAGGCTATTATAGTCTTCCTCATTCCCAAGCTGCAAGGATGTTCTATTTTTTCTTTGAATCTCGAAACACTAAGGATGACCCTGTTGTTATATGGTTAACTGGAGGGCCAGGATGCAGCAGTGAAATTGCTATGTTTTATGAAAATGGTCCTTTTAAATTCTCCAAGGATAAGAACTTGTCCCTTGTTTGGAATGAATATGGATGGGACATGGCATCAAATATAATATTTGTAGACCAACCCACTGGAACAGGTTTCAGCTATACCACTGATGATAGCGACGTTCGACATGATGAAGACGGTGTTAGCAATGATCTATATGATTTCTTGCAGGCATTTTTCAAGGAACACCCTCAGTTCACTAAGAATGATTTTTATATTACCGGAGAATCATACGCTGGACATTACATTCCAGCTTTTGCATCTAGGGTTCATCAAGGAAACAAGGCAAAAGAAGGAATTCATATAAATCTAAAGGGTTTTGCTATTGGTAACGGATTAACCAATCCGGAAATTCAGTACAAGGCATATCCAGATTTTGCATTAGACAATGGACTGATTAATAAGGATGAACATGCCAACATCAACAAGTTGTTCCCACGATGCCAAAAGGCTATAGAATCTTGTGGCACTAAAGGTGGAGACGCATGCATGACTTCAAGAAACATTTGCAGTGAAATATTTGATCAGATCATGGACATAGCCGGCAACATTAATTACTATGACATTAGAAAGCAATGTGTGGGAAGTCTTTGTTATGACTTCTCCAATGCGGAAACATTCTTGAACATGAAGTCAGTAAGGGAAGCTTTAGGTGTTGGGGACTTGGAATTTGTTTCTTGCAGTAGCACAGTATACAGTGCCATGTTGCAGGATTGGATGAAAAATCTAGAAGTGGGTATTCCTGCCCTTCTTGAGGATGGAATCAAGGTGCTTGTGTATGCTGGAGAAGAAGATCTCATATGCAATTGGCTTGGGAATTCGAGGTGGGTTGATGCCATGACATGGTCAGGTCAAAAAGAATTTAAGGCGTCTCCTACAACCCCGTATCTGGTTGATAGTGAAGAAGCAGGAGTTCTGAAAAGCCACGGACCTCTTGCTTTCCTCAAGGTTAAAGAGGCTGGTCACATGGTTCCTATGGATCAACCAAAAGCTGCACTTCAGATGTTAAAAGATTGGATGCAAGGGAAACTGATAAGGACAAGGGGAGGAGATAATGTCTCGTCTATATGA
Protein sequence

>Msa0222420

MASSLRFLQMCLSFVMFFLFISHSFSSPAYPTNNDILLSSTASSYPKLQAENLIRGLNLFPKDSINTPENDPHFVHGNIVEKKFTFPGFVDSGASVEELGHHAGYYSLPHSQAARMFYFFFESRNTKDDPVVIWLTGGPGCSSEIAMFYENGPFKFSKDKNLSLVWNEYGWDMASNIIFVDQPTGTGFSYTTDDSDVRHDEDGVSNDLYDFLQAFFKEHPQFTKNDFYITGESYAGHYIPAFASRVHQGNKAKEGIHINLKGFAIGNGLTNPEIQYKAYPDFALDNGLINKDEHANINKLFPRCQKAIESCGTKGGDACMTSRNICSEIFDQIMDIAGNINYYDIRKQCVGSLCYDFSNAETFLNMKSVREALGVGDLEFVSCSSTVYSAMLQDWMKNLEVGIPALLEDGIKVLVYAGEEDLICNWLGNSRWVDAMTWSGQKEFKASPTTPYLVDSEEAGVLKSHGPLAFLKVKEAGHMVPMDQPKAALQMLKDWMQGKLIRTRGGDNVSSI*