AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0222770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222770 MtrunA17_Chr2g0327831 99.227 388 3 0 1 388 1 388 0.0 805
Msa0222770 MtrunA17_Chr2g0327851 87.761 335 41 0 53 387 1 335 0.0 626
Msa0222770 MtrunA17_Chr1g0212591 53.846 403 161 6 1 386 1 395 9.93e-144 414
Msa0222770 MtrunA17_Chr7g0260171 47.268 366 173 8 30 382 28 386 6.47e-106 318
Msa0222770 MtrunA17_Chr1g0150771 28.933 356 202 13 19 350 167 495 6.43e-34 132
Msa0222770 MtrunA17_Chr4g0070891 26.846 298 190 9 54 345 165 440 6.03e-26 108
Msa0222770 MtrunA17_Chr4g0064951 28.344 314 199 12 78 383 120 415 1.46e-25 107
Msa0222770 MtrunA17_Chr4g0045251 26.974 304 202 10 91 386 149 440 1.12e-24 104
Msa0222770 MtrunA17_Chr4g0044711 23.793 290 200 8 53 341 85 354 8.26e-21 93.2
Msa0222770 MtrunA17_Chr2g0327841 75.510 49 10 1 1 47 1 49 8.27e-14 66.6
Msa0222770 MtrunA17_Chr8g0391981 24.752 303 190 10 54 348 84 356 1.45e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0222770 AT3G55350.1 56.425 358 143 4 34 385 52 402 1.22e-143 414
Msa0222770 AT3G63270.1 44.904 363 174 7 35 382 43 394 1.32e-105 317
Msa0222770 AT5G12010.1 28.037 321 203 8 36 348 161 461 3.57e-34 132
Msa0222770 AT4G29780.1 28.613 346 208 10 22 349 176 500 1.09e-31 126
Msa0222770 AT3G19120.1 24.917 301 212 8 91 386 152 443 1.16e-19 90.9
Msa0222770 AT1G72275.2 33.775 151 95 4 193 342 206 352 1.21e-19 90.5
Msa0222770 AT1G72275.1 33.775 151 95 4 193 342 206 352 1.26e-19 90.5

Find 111 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACCAAGGTTTCTATTT+GGG 0.204482 2_1:-72156420 None:intergenic
TATCTGAAGGATCAGAAATA+AGG 0.227504 2_1:+72155953 Msa0222770:CDS
TTCAACAAACTTTGTGATAA+AGG 0.243513 2_1:+72155901 Msa0222770:CDS
AATAATAATGATTCTGCTTC+TGG 0.256604 2_1:+72152904 Msa0222770:CDS
CACCGGTTGGCCTGGTAAAT+TGG 0.267230 2_1:+72155849 Msa0222770:CDS
AGGTTTGAACATATTTAGTT+AGG 0.268592 2_1:+72156567 Msa0222770:three_prime_UTR
TGGTGACCAAGGTTTCTATT+TGG 0.276611 2_1:-72156421 None:intergenic
GTCACAGTGGGCGATTCATT+TGG 0.281562 2_1:+72155532 Msa0222770:CDS
TTCATTTCCTCTTGTGTTGA+AGG 0.290636 2_1:-72155637 None:intergenic
GAAATAAGGGAATATATAAT+TGG 0.311222 2_1:+72155967 Msa0222770:CDS
GCAATGGTAGCATGCAAATA+AGG 0.313951 2_1:-72152591 None:intergenic
GATAAAGGAGAAAGACTAAA+TGG 0.315467 2_1:+72155916 Msa0222770:CDS
GAATATATAATTGGTGATTC+AGG 0.344534 2_1:+72155976 Msa0222770:CDS
AAGAGTTCTTTCTCTTTGTA+AGG 0.353797 2_1:-72156027 None:intergenic
TCACAGTGGGCGATTCATTT+GGG 0.356270 2_1:+72155533 Msa0222770:CDS
GAATTCTCAAAGAAGATAAA+TGG 0.360311 2_1:+72152964 Msa0222770:CDS
CTAATGTCCATCATTCTCTA+AGG 0.366781 2_1:+72152646 Msa0222770:five_prime_UTR
CAACAGCGCCACAGCAATTC+GGG 0.378156 2_1:-72155696 None:intergenic
GACCGTATGTGCCTAATAGA+AGG 0.388240 2_1:+72156457 Msa0222770:three_prime_UTR
ACTTGAAGTAATTTACTAGA+AGG 0.389645 2_1:+72156623 Msa0222770:three_prime_UTR
TAATCCGTATACCTTCTATT+AGG 0.392065 2_1:-72156468 None:intergenic
TTACAAAGAGAAAGAACTCT+TGG 0.399808 2_1:+72156029 Msa0222770:CDS
TCAACAGCGCCACAGCAATT+CGG 0.407608 2_1:-72155697 None:intergenic
CACCTTCACAAATAAGTTGA+TGG 0.408146 2_1:-72156296 None:intergenic
ATCTGAAGGATCAGAAATAA+GGG 0.416522 2_1:+72155954 Msa0222770:CDS
ATAATAATGATTCTGCTTCT+GGG 0.422150 2_1:+72152905 Msa0222770:CDS
AACGTCTTTCTCGATATCAT+TGG 0.427585 2_1:-72155373 None:intergenic
TGTGCCTAATAGAAGGTATA+CGG 0.427651 2_1:+72156464 Msa0222770:three_prime_UTR
ACAAGTAACATGGCGATTTG+TGG 0.443553 2_1:+72155576 Msa0222770:CDS
AAGAGCATTGACAAGGCTTA+AGG 0.444359 2_1:+72156110 Msa0222770:CDS
CTTGGCAACCGATGTTTGTC+CGG 0.445286 2_1:-72156168 None:intergenic
GACATAGTCACCGGTTGGCC+TGG 0.452018 2_1:+72155841 Msa0222770:CDS
GGTTTGAACATATTTAGTTA+GGG 0.454196 2_1:+72156568 Msa0222770:three_prime_UTR
GTGACCAAGGTTTCTATTTG+GGG 0.456116 2_1:-72156419 None:intergenic
AGTTTGTCTCACTACTTGAA+TGG 0.457626 2_1:+72156348 Msa0222770:CDS
CTCCAATTTACCAGGCCAAC+CGG 0.458165 2_1:-72155851 None:intergenic
TGACTAAGTAAAAGAAATCA+TGG 0.473666 2_1:-72156381 None:intergenic
GAGGGCTTCATCACCTTCAA+TGG 0.473721 2_1:+72155614 Msa0222770:CDS
TATCATAATAATCATGACTC+AGG 0.484139 2_1:+72156270 Msa0222770:CDS
AAAAGATAAAGTAAAGAAAG+AGG 0.491365 2_1:+72156647 Msa0222770:three_prime_UTR
ACAACAAGGTTAAGGTTATG+AGG 0.493234 2_1:-72152527 None:intergenic
ATGAAGCCCTCTGTCCTCCA+TGG 0.493947 2_1:-72155602 None:intergenic
CTTGTGTTGAAGGCCATTGA+AGG 0.504455 2_1:-72155627 None:intergenic
TTCTGGGTCATCTGAGAAAG+AGG 0.509134 2_1:+72152921 Msa0222770:CDS
AAGAGGGTGTTGTAGATTGG+TGG 0.509509 2_1:+72152938 Msa0222770:CDS
ACAACAAGATAAGGCAATAG+AGG 0.511819 2_1:-72156003 None:intergenic
GTCTTTCTCGATATCATTGG+TGG 0.512811 2_1:-72155370 None:intergenic
TGTGAAGGTGTTGATGAGAA+GGG 0.514263 2_1:+72156309 Msa0222770:CDS
GCCGTGGCACTGAGAAGATT+AGG 0.515227 2_1:+72155493 Msa0222770:CDS
TCAAAATTTGAGAAAATACA+AGG 0.516265 2_1:+72155670 Msa0222770:CDS
TAAGTTGTTGTGAATTGTGA+TGG 0.517874 2_1:+72152831 Msa0222770:exon
GGCCTGGTAAATTGGAGGAC+TGG 0.518791 2_1:+72155857 Msa0222770:CDS
TTGTAAGGAACAACAAGATA+AGG 0.522064 2_1:-72156012 None:intergenic
GAAAGAACTCTTGGAGTCAG+AGG 0.524878 2_1:+72156038 Msa0222770:CDS
TTGTGAAGGTGTTGATGAGA+AGG 0.525741 2_1:+72156308 Msa0222770:CDS
AGAAAGAGGGTGTTGTAGAT+TGG 0.528681 2_1:+72152935 Msa0222770:CDS
TCACTCGAATGATCAGTTGC+AGG 0.531246 2_1:-72155745 None:intergenic
TACGGTCGAGGAGATAATCT+TGG 0.534987 2_1:-72156441 None:intergenic
GACCAGTCCTCCAATTTACC+AGG 0.537067 2_1:-72155859 None:intergenic
ATGGCGATTTGTGGAATCCA+TGG 0.540744 2_1:+72155585 Msa0222770:CDS
ACTTGATCAAATAAAGACAT+TGG 0.542893 2_1:-72155469 None:intergenic
GGCGACTCGCTTGTCACAGT+GGG 0.545011 2_1:+72155520 Msa0222770:CDS
CGGTTGGCCTGGTAAATTGG+AGG 0.545258 2_1:+72155852 Msa0222770:CDS
GCACTGAGAAGATTAGGATC+TGG 0.545953 2_1:+72155499 Msa0222770:CDS
ATGTCACGGAACTTCATGTC+AGG 0.546193 2_1:-72155823 None:intergenic
CAACAAACAAGAATAATCCT+TGG 0.547506 2_1:-72156186 None:intergenic
TACCATCAACTTATTTGTGA+AGG 0.547725 2_1:+72156294 Msa0222770:CDS
GCACAAACCTTAGAGAATGA+TGG 0.548599 2_1:-72152653 None:intergenic
TGTCTCACTACTTGAATGGA+AGG 0.551780 2_1:+72156352 Msa0222770:CDS
TGACCAAGGTTTCTATTTGG+GGG 0.559709 2_1:-72156418 None:intergenic
ACAAGCTGCATTTGGCAACA+AGG 0.560506 2_1:+72156076 Msa0222770:CDS
CTATTAGGCACATACGGTCG+AGG 0.566653 2_1:-72156453 None:intergenic
GAATCATTCGTGGAAATATG+TGG 0.568792 2_1:+72156142 Msa0222770:CDS
TGGAATCCATGGAGGACAGA+GGG 0.574505 2_1:+72155596 Msa0222770:CDS
TCGTGGAAATATGTGGCGGC+CGG 0.577956 2_1:+72156149 Msa0222770:CDS
TCCTAATCTTCTCAGTGCCA+CGG 0.582227 2_1:-72155494 None:intergenic
AGAGGGTGTTGTAGATTGGT+GGG 0.589500 2_1:+72152939 Msa0222770:CDS
TGACAAGGCTTAAGGAGATG+TGG 0.589812 2_1:+72156118 Msa0222770:CDS
GCTGCATTTGGCAACAAGGA+TGG 0.589979 2_1:+72156080 Msa0222770:CDS
TACCTTCTATTAGGCACATA+CGG 0.590743 2_1:-72156459 None:intergenic
TACTTGTGAAACAGTTGAGT+AGG 0.593668 2_1:-72155560 None:intergenic
GAGATGTGGAGAATCATTCG+TGG 0.596543 2_1:+72156132 Msa0222770:CDS
CGGACAAACATCGGTTGCCA+AGG 0.597019 2_1:+72156169 Msa0222770:CDS
AAGAAATTAAAATTATCTGA+AGG 0.600968 2_1:+72155940 Msa0222770:CDS
GTACATATGTTCACTTGTCA+AGG 0.602289 2_1:+72155399 Msa0222770:CDS
AAGTTGTTGTGAATTGTGAT+GGG 0.611718 2_1:+72152832 Msa0222770:exon
AAGTTCCGTGACATAGTCAC+CGG 0.612581 2_1:+72155832 Msa0222770:CDS
TCAATGGCCTTCAACACAAG+AGG 0.613766 2_1:+72155630 Msa0222770:CDS
CAAGGCTTCCCGAATTGCTG+TGG 0.614121 2_1:+72155688 Msa0222770:CDS
TGTGGCGGCCGGACAAACAT+CGG 0.615945 2_1:+72156160 Msa0222770:CDS
GGAGATAATCTTGGTGACCA+AGG 0.616974 2_1:-72156432 None:intergenic
CAACTGTTTCACAAGTAACA+TGG 0.626293 2_1:+72155566 Msa0222770:CDS
TCCGTGACATAGTCACCGGT+TGG 0.633391 2_1:+72155836 Msa0222770:CDS
ATCATAATAATCATGACTCA+GGG 0.639066 2_1:+72156271 Msa0222770:CDS
TTTATTTGATCAAGTAGCCG+TGG 0.639843 2_1:+72155477 Msa0222770:CDS
GCCAACCGGTGACTATGTCA+CGG 0.640919 2_1:-72155837 None:intergenic
TGTTACAACAATAAGAGCAA+TGG 0.641798 2_1:-72152607 None:intergenic
GCATTTGGCAACAAGGATGG+TGG 0.641994 2_1:+72156083 Msa0222770:CDS
TCTGGGTCATCTGAGAAAGA+GGG 0.648304 2_1:+72152922 Msa0222770:CDS
TGATATGCAAGATGAAGTGA+AGG 0.651626 2_1:+72156230 Msa0222770:CDS
TGGCGACTCGCTTGTCACAG+TGG 0.652833 2_1:+72155519 Msa0222770:CDS
GTGGAATCCATGGAGGACAG+AGG 0.657784 2_1:+72155595 Msa0222770:CDS
TCATAGAAACAACCACAGCA+TGG 0.659680 2_1:+72155780 Msa0222770:CDS
CTGATCATTCGAGTGATGTG+TGG 0.665693 2_1:+72155752 Msa0222770:CDS
AGGTGTTGATGAGAAGGGAG+TGG 0.668624 2_1:+72156314 Msa0222770:CDS
TTGCTTGCAAAACCATGCTG+TGG 0.669474 2_1:-72155792 None:intergenic
ATTGTGATGGGTCCTGTGAG+AGG 0.681645 2_1:+72152844 Msa0222770:exon
TGGCACAAAGAGCATTGACA+AGG 0.695151 2_1:+72156103 Msa0222770:CDS
GTTTGTAAAAGTCAAATGCG+CGG 0.698464 2_1:-72155440 None:intergenic
GCGATTTGTGGAATCCATGG+AGG 0.702676 2_1:+72155588 Msa0222770:CDS
TCATTCGTGGAAATATGTGG+CGG 0.720354 2_1:+72156145 Msa0222770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAGAAAAAAAAAAAAAAAAA+AGG - chr2_1:72152558-72152577 None:intergenic 10.0%
!!! TTTATTTTAAAACAAGAAAT+TGG - chr2_1:72153557-72153576 None:intergenic 10.0%
!! TTCGTAATTAAAATTTAAAT+TGG - chr2_1:72154148-72154167 None:intergenic 10.0%
!! TAAATCATACATTATTATTT+AGG + chr2_1:72154278-72154297 Msa0222770:intron 10.0%
!!! GTATTTTAATAAATTTTGAT+TGG + chr2_1:72154658-72154677 Msa0222770:intron 10.0%
!! TTATTTAGTATGAAAACATT+TGG - chr2_1:72153532-72153551 None:intergenic 15.0%
!!! AAAATTTCTAAAAATGTGAA+TGG - chr2_1:72154927-72154946 None:intergenic 15.0%
!!! TCACATTTTTAGAAATTTTT+TGG + chr2_1:72154928-72154947 Msa0222770:intron 15.0%
!!! CACATTTTTAGAAATTTTTT+GGG + chr2_1:72154929-72154948 Msa0222770:intron 15.0%
!! AAGAAATTAAAATTATCTGA+AGG + chr2_1:72155940-72155959 Msa0222770:CDS 15.0%
!!! TACTAATTTTGCTATTTATA+AGG + chr2_1:72156547-72156566 Msa0222770:three_prime_UTR 15.0%
!! AAAAAAAGGAAAAAACAACA+AGG - chr2_1:72152544-72152563 None:intergenic 20.0%
!! TTGATTAAATATTCCATGAT+TGG - chr2_1:72153199-72153218 None:intergenic 20.0%
!!! TTGTATCATTGAAATTTTTC+AGG + chr2_1:72154357-72154376 Msa0222770:intron 20.0%
!!! TGTATCATTGAAATTTTTCA+GGG + chr2_1:72154358-72154377 Msa0222770:intron 20.0%
!!! TTTTAACTGAATAAACCAAA+AGG + chr2_1:72154772-72154791 Msa0222770:intron 20.0%
!!! AACTTGTTTAATAACCTTTT+AGG + chr2_1:72154896-72154915 Msa0222770:intron 20.0%
!! AAAAGTCAAACAAAAATACA+AGG + chr2_1:72154954-72154973 Msa0222770:intron 20.0%
!!! GGATATAATACAATTTTAGA+AGG + chr2_1:72155120-72155139 Msa0222770:intron 20.0%
!! TCAAAATTTGAGAAAATACA+AGG + chr2_1:72155670-72155689 Msa0222770:CDS 20.0%
!! GAAATAAGGGAATATATAAT+TGG + chr2_1:72155967-72155986 Msa0222770:CDS 20.0%
!!! ATTGAATCAGAAGTTTTGAA+GGG - chr2_1:72152811-72152830 None:intergenic 25.0%
!!! AATTGAATCAGAAGTTTTGA+AGG - chr2_1:72152812-72152831 None:intergenic 25.0%
! AATAATAATGATTCTGCTTC+TGG + chr2_1:72152904-72152923 Msa0222770:CDS 25.0%
! ATAATAATGATTCTGCTTCT+GGG + chr2_1:72152905-72152924 Msa0222770:CDS 25.0%
! GAATTCTCAAAGAAGATAAA+TGG + chr2_1:72152964-72152983 Msa0222770:CDS 25.0%
! TCAACTTGAAAGTCAAATAA+GGG - chr2_1:72153152-72153171 None:intergenic 25.0%
! ATCAACTTGAAAGTCAAATA+AGG - chr2_1:72153153-72153172 None:intergenic 25.0%
!!! TTTTTTGACAGTTATGTTCA+AGG - chr2_1:72153238-72153257 None:intergenic 25.0%
! CAGTGTATGTTAAATTTCTA+TGG + chr2_1:72153420-72153439 Msa0222770:intron 25.0%
!!! GATATACTAGTTCAGTTTTT+TGG + chr2_1:72153743-72153762 Msa0222770:intron 25.0%
!!! TTGCATTTGTTTTATCTTTG+AGG + chr2_1:72153914-72153933 Msa0222770:intron 25.0%
!! TTATCTTTGAGGAATTTAGT+CGG + chr2_1:72153925-72153944 Msa0222770:intron 25.0%
! AATTTATGTTATCAAGTCCA+AGG + chr2_1:72154066-72154085 Msa0222770:intron 25.0%
! ACAACAATGTTGTAAAATCA+AGG - chr2_1:72154219-72154238 None:intergenic 25.0%
!! TTTACAACATTGTTGTTCAT+TGG + chr2_1:72154223-72154242 Msa0222770:intron 25.0%
! TCTACAAAAAAACATCAAGT+TGG - chr2_1:72154401-72154420 None:intergenic 25.0%
!! AAAAAGGTTTTCAGTAAACA+TGG - chr2_1:72154513-72154532 None:intergenic 25.0%
! TCAAGTAATTCTACTGAAAA+AGG - chr2_1:72154529-72154548 None:intergenic 25.0%
! AGATTTGAAAACTGAATACT+GGG - chr2_1:72154588-72154607 None:intergenic 25.0%
! AAGATTTGAAAACTGAATAC+TGG - chr2_1:72154589-72154608 None:intergenic 25.0%
!! TTGTAAGTTGTTAAACCTTT+TGG - chr2_1:72154790-72154809 None:intergenic 25.0%
! TAACCTATAAACATGTATAG+CGG - chr2_1:72154829-72154848 None:intergenic 25.0%
! TAACATAAACCTGCATAAAA+TGG - chr2_1:72154985-72155004 None:intergenic 25.0%
! CTCACATATTTAGTAATCTT+TGG + chr2_1:72155018-72155037 Msa0222770:intron 25.0%
! ACTTGATCAAATAAAGACAT+TGG - chr2_1:72155472-72155491 None:intergenic 25.0%
! TTCAACAAACTTTGTGATAA+AGG + chr2_1:72155901-72155920 Msa0222770:CDS 25.0%
!! GAATATATAATTGGTGATTC+AGG + chr2_1:72155976-72155995 Msa0222770:CDS 25.0%
! TATCATAATAATCATGACTC+AGG + chr2_1:72156270-72156289 Msa0222770:CDS 25.0%
! ATCATAATAATCATGACTCA+GGG + chr2_1:72156271-72156290 Msa0222770:CDS 25.0%
! TGACTAAGTAAAAGAAATCA+TGG - chr2_1:72156384-72156403 None:intergenic 25.0%
!!! TATGTTGTTTTTAGATGTGA+AGG + chr2_1:72156491-72156510 Msa0222770:three_prime_UTR 25.0%
! AGGTTTGAACATATTTAGTT+AGG + chr2_1:72156567-72156586 Msa0222770:three_prime_UTR 25.0%
! GGTTTGAACATATTTAGTTA+GGG + chr2_1:72156568-72156587 Msa0222770:three_prime_UTR 25.0%
! ACTTGAAGTAATTTACTAGA+AGG + chr2_1:72156623-72156642 Msa0222770:three_prime_UTR 25.0%
AGGAAAAAACAACAAGGTTA+AGG - chr2_1:72152538-72152557 None:intergenic 30.0%
TGTTACAACAATAAGAGCAA+TGG - chr2_1:72152610-72152629 None:intergenic 30.0%
TCTGCAAGAAAGAATTCAAA+GGG - chr2_1:72152779-72152798 None:intergenic 30.0%
ATCTGCAAGAAAGAATTCAA+AGG - chr2_1:72152780-72152799 None:intergenic 30.0%
!!! TTGAATCAGAAGTTTTGAAG+GGG - chr2_1:72152810-72152829 None:intergenic 30.0%
TAAGTTGTTGTGAATTGTGA+TGG + chr2_1:72152831-72152850 Msa0222770:exon 30.0%
AAGTTGTTGTGAATTGTGAT+GGG + chr2_1:72152832-72152851 Msa0222770:exon 30.0%
!!! GATTTTTTGCTGATGAATCA+TGG + chr2_1:72153065-72153084 Msa0222770:intron 30.0%
CAACTTGAAAGTCAAATAAG+GGG - chr2_1:72153151-72153170 None:intergenic 30.0%
ATGTGTTATCTTACCAATCA+TGG + chr2_1:72153183-72153202 Msa0222770:intron 30.0%
GAGTTTAAGAATGAGGTTTA+GGG - chr2_1:72153338-72153357 None:intergenic 30.0%
GTAACTTAAGTGGTTAATTC+AGG - chr2_1:72153371-72153390 None:intergenic 30.0%
!! TGTGATTTCTGGTTTATATG+AGG + chr2_1:72153476-72153495 Msa0222770:intron 30.0%
TTGAGCAATACAGAATATGT+AGG - chr2_1:72153784-72153803 None:intergenic 30.0%
TTATCAAGTCCAAGGAAAAA+AGG + chr2_1:72154074-72154093 Msa0222770:intron 30.0%
AAAGCAAATTACTTGCAAAC+TGG - chr2_1:72154732-72154751 None:intergenic 30.0%
AACAAATTGTACTACTGTGT+AGG + chr2_1:72154854-72154873 Msa0222770:intron 30.0%
! AAGGTAATACCATTTTATGC+AGG + chr2_1:72154973-72154992 Msa0222770:intron 30.0%
!! TCAAGGATCATGTTATTTCT+AGG + chr2_1:72155087-72155106 Msa0222770:intron 30.0%
!! ATCATGTTATTTCTAGGAAC+AGG + chr2_1:72155093-72155112 Msa0222770:intron 30.0%
!! TTATTTCTAGGAACAGGAAT+TGG + chr2_1:72155099-72155118 Msa0222770:intron 30.0%
GATAAAGGAGAAAGACTAAA+TGG + chr2_1:72155916-72155935 Msa0222770:CDS 30.0%
TATCTGAAGGATCAGAAATA+AGG + chr2_1:72155953-72155972 Msa0222770:CDS 30.0%
ATCTGAAGGATCAGAAATAA+GGG + chr2_1:72155954-72155973 Msa0222770:CDS 30.0%
TTGTAAGGAACAACAAGATA+AGG - chr2_1:72156015-72156034 None:intergenic 30.0%
AAGAGTTCTTTCTCTTTGTA+AGG - chr2_1:72156030-72156049 None:intergenic 30.0%
TTACAAAGAGAAAGAACTCT+TGG + chr2_1:72156029-72156048 Msa0222770:CDS 30.0%
!! TCATTTTAACAAGCTGCATT+TGG + chr2_1:72156068-72156087 Msa0222770:CDS 30.0%
CAACAAACAAGAATAATCCT+TGG - chr2_1:72156189-72156208 None:intergenic 30.0%
TACCATCAACTTATTTGTGA+AGG + chr2_1:72156294-72156313 Msa0222770:CDS 30.0%
TAATCCGTATACCTTCTATT+AGG - chr2_1:72156471-72156490 None:intergenic 30.0%
ACAACAAGGTTAAGGTTATG+AGG - chr2_1:72152530-72152549 None:intergenic 35.0%
CTAATGTCCATCATTCTCTA+AGG + chr2_1:72152646-72152665 Msa0222770:five_prime_UTR 35.0%
!! GTTTGCTATGATGAATGTGT+TGG + chr2_1:72153087-72153106 Msa0222770:intron 35.0%
GGAGTTTAAGAATGAGGTTT+AGG - chr2_1:72153339-72153358 None:intergenic 35.0%
!!! GGAGATAGAAGCTTTTTTCT+TGG + chr2_1:72153441-72153460 Msa0222770:intron 35.0%
TGTAATCTGTCTGTGATTTC+TGG + chr2_1:72153465-72153484 Msa0222770:intron 35.0%
CATATTCTGTATTGCTCAAG+TGG + chr2_1:72153785-72153804 Msa0222770:intron 35.0%
CAAGTGGTTAATTTAAGCCT+AGG + chr2_1:72153801-72153820 Msa0222770:intron 35.0%
GCAATTAGTATGCTTGTAGA+AGG + chr2_1:72153842-72153861 Msa0222770:intron 35.0%
! TTTGATTCGTCCAACAAAAC+CGG - chr2_1:72153954-72153973 None:intergenic 35.0%
TTGTTGGACGAATCAAAATG+AGG + chr2_1:72153957-72153976 Msa0222770:intron 35.0%
AATTATCACATCATGTCCTG+TGG + chr2_1:72153991-72154010 Msa0222770:intron 35.0%
ATGAATTCTATTTCCTCCAC+AGG - chr2_1:72154010-72154029 None:intergenic 35.0%
CATGATGATCATATGTCACA+AGG - chr2_1:72154457-72154476 None:intergenic 35.0%
CTTGTGACATATGATCATCA+TGG + chr2_1:72154455-72154474 Msa0222770:intron 35.0%
AATTGTACTACTGTGTAGGT+TGG + chr2_1:72154858-72154877 Msa0222770:intron 35.0%
TGTGTGATCATTAGTGATGT+CGG + chr2_1:72155052-72155071 Msa0222770:intron 35.0%
!! AACACAAGTTCTAAGGTTTG+AGG - chr2_1:72155327-72155346 None:intergenic 35.0%
! ACCTTAGAACTTGTGTTGAT+TGG + chr2_1:72155330-72155349 Msa0222770:intron 35.0%
AACGTCTTTCTCGATATCAT+TGG - chr2_1:72155376-72155395 None:intergenic 35.0%
GTACATATGTTCACTTGTCA+AGG + chr2_1:72155399-72155418 Msa0222770:CDS 35.0%
GTTTGTAAAAGTCAAATGCG+CGG - chr2_1:72155443-72155462 None:intergenic 35.0%
!! GCGCATTTGACTTTTACAAA+CGG + chr2_1:72155442-72155461 Msa0222770:CDS 35.0%
TTTATTTGATCAAGTAGCCG+TGG + chr2_1:72155477-72155496 Msa0222770:CDS 35.0%
TACTTGTGAAACAGTTGAGT+AGG - chr2_1:72155563-72155582 None:intergenic 35.0%
CAACTGTTTCACAAGTAACA+TGG + chr2_1:72155566-72155585 Msa0222770:CDS 35.0%
TTCATTTCCTCTTGTGTTGA+AGG - chr2_1:72155640-72155659 None:intergenic 35.0%
ACAACAAGATAAGGCAATAG+AGG - chr2_1:72156006-72156025 None:intergenic 35.0%
GAATCATTCGTGGAAATATG+TGG + chr2_1:72156142-72156161 Msa0222770:CDS 35.0%
TGATATGCAAGATGAAGTGA+AGG + chr2_1:72156230-72156249 Msa0222770:CDS 35.0%
! CACCTTCACAAATAAGTTGA+TGG - chr2_1:72156299-72156318 None:intergenic 35.0%
AGTTTGTCTCACTACTTGAA+TGG + chr2_1:72156348-72156367 Msa0222770:CDS 35.0%
TACCTTCTATTAGGCACATA+CGG - chr2_1:72156462-72156481 None:intergenic 35.0%
! TGTGCCTAATAGAAGGTATA+CGG + chr2_1:72156464-72156483 Msa0222770:three_prime_UTR 35.0%
AGGTGAGAAAAACACAATAG+CGG + chr2_1:72156511-72156530 Msa0222770:three_prime_UTR 35.0%
GCAATGGTAGCATGCAAATA+AGG - chr2_1:72152594-72152613 None:intergenic 40.0%
GCACAAACCTTAGAGAATGA+TGG - chr2_1:72152656-72152675 None:intergenic 40.0%
!! CTCTTTTTGATTCCTCTCAC+AGG - chr2_1:72152859-72152878 None:intergenic 40.0%
!! AGAAAGAGGGTGTTGTAGAT+TGG + chr2_1:72152935-72152954 Msa0222770:CDS 40.0%
ACCTCATTCTTAAACTCCTG+CGG + chr2_1:72153341-72153360 Msa0222770:intron 40.0%
TAACCACTTAAGTTACGCCA+TGG + chr2_1:72153375-72153394 Msa0222770:intron 40.0%
AACCACTTAAGTTACGCCAT+GGG + chr2_1:72153376-72153395 Msa0222770:intron 40.0%
AATGTACTATGTAGTCCCCA+TGG - chr2_1:72153395-72153414 None:intergenic 40.0%
GCATACTAATTGCTACCATC+AGG - chr2_1:72153835-72153854 None:intergenic 40.0%
! TTGATTCGTCCAACAAAACC+GGG - chr2_1:72153953-72153972 None:intergenic 40.0%
!!! TTGACTGCACCTTTTTTCCT+TGG - chr2_1:72154086-72154105 None:intergenic 40.0%
GACAAATCGACAATGAACCA+AGG + chr2_1:72154306-72154325 Msa0222770:intron 40.0%
GAACCAAGGCTTTCAATTCT+TGG + chr2_1:72154320-72154339 Msa0222770:intron 40.0%
GAACCAAGAATTGAAAGCCT+TGG - chr2_1:72154326-72154345 None:intergenic 40.0%
GTGACATATGATCATCATGG+TGG + chr2_1:72154458-72154477 Msa0222770:intron 40.0%
! CATGGTCTGGTTTCAGTTAA+GGG - chr2_1:72154495-72154514 None:intergenic 40.0%
! ACATGGTCTGGTTTCAGTTA+AGG - chr2_1:72154496-72154515 None:intergenic 40.0%
! GGTTTTCAGTAAACATGGTC+TGG - chr2_1:72154508-72154527 None:intergenic 40.0%
CAGCCGCTATACATGTTTAT+AGG + chr2_1:72154823-72154842 Msa0222770:intron 40.0%
! TGTGAATGGCTAGTCCTAAA+AGG - chr2_1:72154913-72154932 None:intergenic 40.0%
TGATCATTAGTGATGTCGGT+TGG + chr2_1:72155056-72155075 Msa0222770:intron 40.0%
!! GTCGGTTGGTACATTTTTCA+AGG + chr2_1:72155070-72155089 Msa0222770:intron 40.0%
TGTGAGAAAACCTCATCTGA+CGG - chr2_1:72155146-72155165 None:intergenic 40.0%
GCCAATCAACACAAGTTCTA+AGG - chr2_1:72155334-72155353 None:intergenic 40.0%
!! TAGAACTTGTGTTGATTGGC+AGG + chr2_1:72155334-72155353 Msa0222770:intron 40.0%
GTCTTTCTCGATATCATTGG+TGG - chr2_1:72155373-72155392 None:intergenic 40.0%
ACAAGTAACATGGCGATTTG+TGG + chr2_1:72155576-72155595 Msa0222770:CDS 40.0%
TCATAGAAACAACCACAGCA+TGG + chr2_1:72155780-72155799 Msa0222770:CDS 40.0%
!! AAGAGCATTGACAAGGCTTA+AGG + chr2_1:72156110-72156129 Msa0222770:CDS 40.0%
TCATTCGTGGAAATATGTGG+CGG + chr2_1:72156145-72156164 Msa0222770:CDS 40.0%
!! TTGTGAAGGTGTTGATGAGA+AGG + chr2_1:72156308-72156327 Msa0222770:CDS 40.0%
!! TGTGAAGGTGTTGATGAGAA+GGG + chr2_1:72156309-72156328 Msa0222770:CDS 40.0%
TGTCTCACTACTTGAATGGA+AGG + chr2_1:72156352-72156371 Msa0222770:CDS 40.0%
TGACCAAGGTTTCTATTTGG+GGG - chr2_1:72156421-72156440 None:intergenic 40.0%
GTGACCAAGGTTTCTATTTG+GGG - chr2_1:72156422-72156441 None:intergenic 40.0%
GGTGACCAAGGTTTCTATTT+GGG - chr2_1:72156423-72156442 None:intergenic 40.0%
TGGTGACCAAGGTTTCTATT+TGG - chr2_1:72156424-72156443 None:intergenic 40.0%
GGTGAGAAAAACACAATAGC+GGG + chr2_1:72156512-72156531 Msa0222770:three_prime_UTR 40.0%
GTGAGAAAAACACAATAGCG+GGG + chr2_1:72156513-72156532 Msa0222770:three_prime_UTR 40.0%
TTCTGGGTCATCTGAGAAAG+AGG + chr2_1:72152921-72152940 Msa0222770:CDS 45.0%
TCTGGGTCATCTGAGAAAGA+GGG + chr2_1:72152922-72152941 Msa0222770:CDS 45.0%
!! AAGAGGGTGTTGTAGATTGG+TGG + chr2_1:72152938-72152957 Msa0222770:CDS 45.0%
!! AGAGGGTGTTGTAGATTGGT+GGG + chr2_1:72152939-72152958 Msa0222770:CDS 45.0%
CCTCATTCTTAAACTCCTGC+GGG + chr2_1:72153342-72153361 Msa0222770:intron 45.0%
ACCACTTAAGTTACGCCATG+GGG + chr2_1:72153377-72153396 Msa0222770:intron 45.0%
ACCATCAGGCTGATATACCT+AGG - chr2_1:72153821-72153840 None:intergenic 45.0%
! TGAGGAATTTAGTCGGTTCC+CGG + chr2_1:72153932-72153951 Msa0222770:intron 45.0%
TATCACATCATGTCCTGTGG+AGG + chr2_1:72153994-72154013 Msa0222770:intron 45.0%
!! TTTTAGAAGGCCGTCAGATG+AGG + chr2_1:72155133-72155152 Msa0222770:intron 45.0%
GTTATGCATTGCTGCAACTC+AGG + chr2_1:72155208-72155227 Msa0222770:intron 45.0%
TCCTAATCTTCTCAGTGCCA+CGG - chr2_1:72155497-72155516 None:intergenic 45.0%
GCACTGAGAAGATTAGGATC+TGG + chr2_1:72155499-72155518 Msa0222770:CDS 45.0%
TCACAGTGGGCGATTCATTT+GGG + chr2_1:72155533-72155552 Msa0222770:CDS 45.0%
ATGGCGATTTGTGGAATCCA+TGG + chr2_1:72155585-72155604 Msa0222770:CDS 45.0%
CTTGTGTTGAAGGCCATTGA+AGG - chr2_1:72155630-72155649 None:intergenic 45.0%
TCAATGGCCTTCAACACAAG+AGG + chr2_1:72155630-72155649 Msa0222770:CDS 45.0%
TCACTCGAATGATCAGTTGC+AGG - chr2_1:72155748-72155767 None:intergenic 45.0%
CTGATCATTCGAGTGATGTG+TGG + chr2_1:72155752-72155771 Msa0222770:CDS 45.0%
TTGCTTGCAAAACCATGCTG+TGG - chr2_1:72155795-72155814 None:intergenic 45.0%
ATGTCACGGAACTTCATGTC+AGG - chr2_1:72155826-72155845 None:intergenic 45.0%
AAGTTCCGTGACATAGTCAC+CGG + chr2_1:72155832-72155851 Msa0222770:CDS 45.0%
GAAAGAACTCTTGGAGTCAG+AGG + chr2_1:72156038-72156057 Msa0222770:CDS 45.0%
ACAAGCTGCATTTGGCAACA+AGG + chr2_1:72156076-72156095 Msa0222770:CDS 45.0%
!! TGGCACAAAGAGCATTGACA+AGG + chr2_1:72156103-72156122 Msa0222770:CDS 45.0%
TGACAAGGCTTAAGGAGATG+TGG + chr2_1:72156118-72156137 Msa0222770:CDS 45.0%
GAGATGTGGAGAATCATTCG+TGG + chr2_1:72156132-72156151 Msa0222770:CDS 45.0%
TAGCCCCCAAATAGAAACCT+TGG + chr2_1:72156415-72156434 Msa0222770:three_prime_UTR 45.0%
! GGAGATAATCTTGGTGACCA+AGG - chr2_1:72156435-72156454 None:intergenic 45.0%
TACGGTCGAGGAGATAATCT+TGG - chr2_1:72156444-72156463 None:intergenic 45.0%
GACCGTATGTGCCTAATAGA+AGG + chr2_1:72156457-72156476 Msa0222770:three_prime_UTR 45.0%
!! ATTGTGATGGGTCCTGTGAG+AGG + chr2_1:72152844-72152863 Msa0222770:exon 50.0%
CCCGCAGGAGTTTAAGAATG+AGG - chr2_1:72153345-72153364 None:intergenic 50.0%
GGTTAATTCAGGATACCCGC+AGG - chr2_1:72153360-72153379 None:intergenic 50.0%
TCCCCATGGCGTAACTTAAG+TGG - chr2_1:72153381-72153400 None:intergenic 50.0%
GCCTAGGTATATCAGCCTGA+TGG + chr2_1:72153817-72153836 Msa0222770:intron 50.0%
!! TAGTCGGTTCCCGGTTTTGT+TGG + chr2_1:72153941-72153960 Msa0222770:intron 50.0%
GTCACAGTGGGCGATTCATT+TGG + chr2_1:72155532-72155551 Msa0222770:CDS 50.0%
GCGATTTGTGGAATCCATGG+AGG + chr2_1:72155588-72155607 Msa0222770:CDS 50.0%
TGGAATCCATGGAGGACAGA+GGG + chr2_1:72155596-72155615 Msa0222770:CDS 50.0%
GAGGGCTTCATCACCTTCAA+TGG + chr2_1:72155614-72155633 Msa0222770:CDS 50.0%
TCAACAGCGCCACAGCAATT+CGG - chr2_1:72155700-72155719 None:intergenic 50.0%
CTCCAATTTACCAGGCCAAC+CGG - chr2_1:72155854-72155873 None:intergenic 50.0%
GACCAGTCCTCCAATTTACC+AGG - chr2_1:72155862-72155881 None:intergenic 50.0%
GCTGCATTTGGCAACAAGGA+TGG + chr2_1:72156080-72156099 Msa0222770:CDS 50.0%
GCATTTGGCAACAAGGATGG+TGG + chr2_1:72156083-72156102 Msa0222770:CDS 50.0%
CTTGGCAACCGATGTTTGTC+CGG - chr2_1:72156171-72156190 None:intergenic 50.0%
!! AGGTGTTGATGAGAAGGGAG+TGG + chr2_1:72156314-72156333 Msa0222770:CDS 50.0%
CTATTAGGCACATACGGTCG+AGG - chr2_1:72156456-72156475 None:intergenic 50.0%
! GCCGTGGCACTGAGAAGATT+AGG + chr2_1:72155493-72155512 Msa0222770:CDS 55.0%
GTGGAATCCATGGAGGACAG+AGG + chr2_1:72155595-72155614 Msa0222770:CDS 55.0%
ATGAAGCCCTCTGTCCTCCA+TGG - chr2_1:72155605-72155624 None:intergenic 55.0%
CAAGGCTTCCCGAATTGCTG+TGG + chr2_1:72155688-72155707 Msa0222770:CDS 55.0%
CAACAGCGCCACAGCAATTC+GGG - chr2_1:72155699-72155718 None:intergenic 55.0%
TCCGTGACATAGTCACCGGT+TGG + chr2_1:72155836-72155855 Msa0222770:CDS 55.0%
GCCAACCGGTGACTATGTCA+CGG - chr2_1:72155840-72155859 None:intergenic 55.0%
!! CACCGGTTGGCCTGGTAAAT+TGG + chr2_1:72155849-72155868 Msa0222770:CDS 55.0%
!! CGGTTGGCCTGGTAAATTGG+AGG + chr2_1:72155852-72155871 Msa0222770:CDS 55.0%
GGCCTGGTAAATTGGAGGAC+TGG + chr2_1:72155857-72155876 Msa0222770:CDS 55.0%
TCGTGGAAATATGTGGCGGC+CGG + chr2_1:72156149-72156168 Msa0222770:CDS 55.0%
! CGGACAAACATCGGTTGCCA+AGG + chr2_1:72156169-72156188 Msa0222770:CDS 55.0%
TGGCGACTCGCTTGTCACAG+TGG + chr2_1:72155519-72155538 Msa0222770:CDS 60.0%
GGCGACTCGCTTGTCACAGT+GGG + chr2_1:72155520-72155539 Msa0222770:CDS 60.0%
! GACATAGTCACCGGTTGGCC+TGG + chr2_1:72155841-72155860 Msa0222770:CDS 60.0%
TGTGGCGGCCGGACAAACAT+CGG + chr2_1:72156160-72156179 Msa0222770:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 72152481 72156666 72152481 ID=Msa0222770;Name=Msa0222770
chr2_1 mRNA 72152481 72156666 72152481 ID=Msa0222770-mRNA-1;Parent=Msa0222770;Name=Msa0222770-mRNA-1;_AED=0.24;_eAED=0.24;_QI=239|1|1|1|0.5|0.33|3|280|388
chr2_1 exon 72152481 72152667 72152481 ID=Msa0222770-mRNA-1:exon:20832;Parent=Msa0222770-mRNA-1
chr2_1 exon 72152798 72152985 72152798 ID=Msa0222770-mRNA-1:exon:20833;Parent=Msa0222770-mRNA-1
chr2_1 exon 72155356 72156666 72155356 ID=Msa0222770-mRNA-1:exon:20834;Parent=Msa0222770-mRNA-1
chr2_1 five_prime_UTR 72152481 72152667 72152481 ID=Msa0222770-mRNA-1:five_prime_utr;Parent=Msa0222770-mRNA-1
chr2_1 five_prime_UTR 72152798 72152849 72152798 ID=Msa0222770-mRNA-1:five_prime_utr;Parent=Msa0222770-mRNA-1
chr2_1 CDS 72152850 72152985 72152850 ID=Msa0222770-mRNA-1:cds;Parent=Msa0222770-mRNA-1
chr2_1 CDS 72155356 72156386 72155356 ID=Msa0222770-mRNA-1:cds;Parent=Msa0222770-mRNA-1
chr2_1 three_prime_UTR 72156387 72156666 72156387 ID=Msa0222770-mRNA-1:three_prime_utr;Parent=Msa0222770-mRNA-1
Gene Sequence

>Msa0222770

ATGGGTCCTGTGAGAGGAATCAAAAAGAGAAGAAAGACAGAGAAGAGTTATGACAATAATAATGATTCTGCTTCTGGGTCATCTGAGAAAGAGGGTGTTGTAGATTGGTGGGATGAATTCTCAAAGAAGATAAATGGTCTTCAGAAGTCACCACCAATGATATCGAGAAAGACGTTTGAGTACATATGTTCACTTGTCAAGGACGATATGACAACAAAATCCGCGCATTTGACTTTTACAAACGGAAAGCCAATGTCTTTATTTGATCAAGTAGCCGTGGCACTGAGAAGATTAGGATCTGGCGACTCGCTTGTCACAGTGGGCGATTCATTTGGGCTAACCTACTCAACTGTTTCACAAGTAACATGGCGATTTGTGGAATCCATGGAGGACAGAGGGCTTCATCACCTTCAATGGCCTTCAACACAAGAGGAAATGAATGCAATCAAGTCAAAATTTGAGAAAATACAAGGCTTCCCGAATTGCTGTGGCGCTGTTGATGCTACTCACATCACAATGTGTTTACCTGCAACTGATCATTCGAGTGATGTGTGGCTTGATCATAGAAACAACCACAGCATGGTTTTGCAAGCAATAGTTGATCCTGACATGAAGTTCCGTGACATAGTCACCGGTTGGCCTGGTAAATTGGAGGACTGGTCAATATTCGAGAGTTCAAATTTCAACAAACTTTGTGATAAAGGAGAAAGACTAAATGGAAAGAAATTAAAATTATCTGAAGGATCAGAAATAAGGGAATATATAATTGGTGATTCAGGTTATCCTCTATTGCCTTATCTTGTTGTTCCTTACAAAGAGAAAGAACTCTTGGAGTCAGAGGCAAAAACTCATTTTAACAAGCTGCATTTGGCAACAAGGATGGTGGCACAAAGAGCATTGACAAGGCTTAAGGAGATGTGGAGAATCATTCGTGGAAATATGTGGCGGCCGGACAAACATCGGTTGCCAAGGATTATTCTTGTTTGTTGCTTACTTCACAACATTGTCATTGATATGCAAGATGAAGTGAAGGATGAATTGCTCTATTTGTATCATAATAATCATGACTCAGGGTACCATCAACTTATTTGTGAAGGTGTTGATGAGAAGGGAGTGGCACTAAGAGAAAGTTTGTCTCACTACTTGAATGGAAGGTTGCATCCATGA
Protein sequence

>Msa0222770

MGPVRGIKKRRKTEKSYDNNNDSASGSSEKEGVVDWWDEFSKKINGLQKSPPMISRKTFEYICSLVKDDMTTKSAHLTFTNGKPMSLFDQVAVALRRLGSGDSLVTVGDSFGLTYSTVSQVTWRFVESMEDRGLHHLQWPSTQEEMNAIKSKFEKIQGFPNCCGAVDATHITMCLPATDHSSDVWLDHRNNHSMVLQAIVDPDMKFRDIVTGWPGKLEDWSIFESSNFNKLCDKGERLNGKKLKLSEGSEIREYIIGDSGYPLLPYLVVPYKEKELLESEAKTHFNKLHLATRMVAQRALTRLKEMWRIIRGNMWRPDKHRLPRIILVCCLLHNIVIDMQDEVKDELLYLYHNNHDSGYHQLICEGVDEKGVALRESLSHYLNGRLHP*