AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0339390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339390 MtrunA17_Chr2g0327831 99.227 388 3 0 1 388 1 388 0.0 805
Msa0339390 MtrunA17_Chr2g0327851 87.761 335 41 0 53 387 1 335 0.0 626
Msa0339390 MtrunA17_Chr1g0212591 53.846 403 161 6 1 386 1 395 9.93e-144 414
Msa0339390 MtrunA17_Chr7g0260171 47.268 366 173 8 30 382 28 386 6.47e-106 318
Msa0339390 MtrunA17_Chr1g0150771 28.933 356 202 13 19 350 167 495 6.43e-34 132
Msa0339390 MtrunA17_Chr4g0070891 26.846 298 190 9 54 345 165 440 6.03e-26 108
Msa0339390 MtrunA17_Chr4g0064951 28.344 314 199 12 78 383 120 415 1.46e-25 107
Msa0339390 MtrunA17_Chr4g0045251 26.974 304 202 10 91 386 149 440 1.12e-24 104
Msa0339390 MtrunA17_Chr4g0044711 23.793 290 200 8 53 341 85 354 8.26e-21 93.2
Msa0339390 MtrunA17_Chr2g0327841 75.510 49 10 1 1 47 1 49 8.27e-14 66.6
Msa0339390 MtrunA17_Chr8g0391981 24.752 303 190 10 54 348 84 356 1.45e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0339390 AT3G55350.1 56.425 358 143 4 34 385 52 402 1.22e-143 414
Msa0339390 AT3G63270.1 44.904 363 174 7 35 382 43 394 1.32e-105 317
Msa0339390 AT5G12010.1 28.037 321 203 8 36 348 161 461 3.57e-34 132
Msa0339390 AT4G29780.1 28.613 346 208 10 22 349 176 500 1.09e-31 126
Msa0339390 AT3G19120.1 24.917 301 212 8 91 386 152 443 1.16e-19 90.9
Msa0339390 AT1G72275.2 33.775 151 95 4 193 342 206 352 1.21e-19 90.5
Msa0339390 AT1G72275.1 33.775 151 95 4 193 342 206 352 1.26e-19 90.5

Find 111 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACCAAGGTTTCTATTT+GGG 0.204482 2_4:-60552180 None:intergenic
TATCTGAAGGATCAGAAATA+AGG 0.227504 2_4:+60551713 Msa0339390:CDS
TTCAACAAACTTTGTGATAA+AGG 0.243513 2_4:+60551661 Msa0339390:CDS
AATAATAATGATTCTGCTTC+TGG 0.256604 2_4:+60548732 Msa0339390:CDS
CACCGGTTGGCCTGGTAAAT+TGG 0.267230 2_4:+60551609 Msa0339390:CDS
AGGTTTGAACATATTTAGTT+AGG 0.268592 2_4:+60552327 Msa0339390:three_prime_UTR
TGGTGACCAAGGTTTCTATT+TGG 0.276611 2_4:-60552181 None:intergenic
GTCACAGTGGGCGATTCATT+TGG 0.281562 2_4:+60551292 Msa0339390:CDS
TTCATTTCCTCTTGTGTTGA+AGG 0.290636 2_4:-60551397 None:intergenic
GAAATAAGGGAATATATAAT+TGG 0.311222 2_4:+60551727 Msa0339390:CDS
GCAATGGTAGCATGCAAATA+AGG 0.313951 2_4:-60548409 None:intergenic
GATAAAGGAGAAAGACTAAA+TGG 0.315467 2_4:+60551676 Msa0339390:CDS
GAATATATAATTGGTGATTC+AGG 0.344534 2_4:+60551736 Msa0339390:CDS
AAGAGTTCTTTCTCTTTGTA+AGG 0.353797 2_4:-60551787 None:intergenic
TCACAGTGGGCGATTCATTT+GGG 0.356270 2_4:+60551293 Msa0339390:CDS
GAATTCTCAAAGAAGATAAA+TGG 0.360311 2_4:+60548792 Msa0339390:CDS
CTAATGTCCATCATTCTCTA+AGG 0.366781 2_4:+60548464 Msa0339390:five_prime_UTR
CAACAGCGCCACAGCAATTC+GGG 0.378156 2_4:-60551456 None:intergenic
GACCGTATGTGCCTAATAGA+AGG 0.388240 2_4:+60552217 Msa0339390:three_prime_UTR
ACTTGAAGTAATTTACTAGA+AGG 0.389645 2_4:+60552383 Msa0339390:three_prime_UTR
TAATCCGTATACCTTCTATT+AGG 0.392065 2_4:-60552228 None:intergenic
TTACAAAGAGAAAGAACTCT+TGG 0.399808 2_4:+60551789 Msa0339390:CDS
TCAACAGCGCCACAGCAATT+CGG 0.407608 2_4:-60551457 None:intergenic
CACCTTCACAAATAAGTTGA+TGG 0.408146 2_4:-60552056 None:intergenic
ATCTGAAGGATCAGAAATAA+GGG 0.416522 2_4:+60551714 Msa0339390:CDS
ATAATAATGATTCTGCTTCT+GGG 0.422150 2_4:+60548733 Msa0339390:CDS
AACGTCTTTCTCGATATCAT+TGG 0.427585 2_4:-60551133 None:intergenic
TGTGCCTAATAGAAGGTATA+CGG 0.427651 2_4:+60552224 Msa0339390:three_prime_UTR
ACAAGTAACATGGCGATTTG+TGG 0.443553 2_4:+60551336 Msa0339390:CDS
AAGAGCATTGACAAGGCTTA+AGG 0.444359 2_4:+60551870 Msa0339390:CDS
CTTGGCAACCGATGTTTGTC+CGG 0.445286 2_4:-60551928 None:intergenic
GACATAGTCACCGGTTGGCC+TGG 0.452018 2_4:+60551601 Msa0339390:CDS
GGTTTGAACATATTTAGTTA+GGG 0.454196 2_4:+60552328 Msa0339390:three_prime_UTR
GTGACCAAGGTTTCTATTTG+GGG 0.456116 2_4:-60552179 None:intergenic
AGTTTGTCTCACTACTTGAA+TGG 0.457626 2_4:+60552108 Msa0339390:CDS
CTCCAATTTACCAGGCCAAC+CGG 0.458165 2_4:-60551611 None:intergenic
TGACTAAGTAAAAGAAATCA+TGG 0.473666 2_4:-60552141 None:intergenic
GAGGGCTTCATCACCTTCAA+TGG 0.473721 2_4:+60551374 Msa0339390:CDS
TATCATAATAATCATGACTC+AGG 0.484139 2_4:+60552030 Msa0339390:CDS
AAAAGATAAAGTAAAGAAAG+AGG 0.491365 2_4:+60552407 Msa0339390:three_prime_UTR
ACAACAAGGTTAAGGTTATG+AGG 0.493234 2_4:-60548353 None:intergenic
ATGAAGCCCTCTGTCCTCCA+TGG 0.493947 2_4:-60551362 None:intergenic
CTTGTGTTGAAGGCCATTGA+AGG 0.504455 2_4:-60551387 None:intergenic
TTCTGGGTCATCTGAGAAAG+AGG 0.509134 2_4:+60548749 Msa0339390:CDS
AAGAGGGTGTTGTAGATTGG+TGG 0.509509 2_4:+60548766 Msa0339390:CDS
ACAACAAGATAAGGCAATAG+AGG 0.511819 2_4:-60551763 None:intergenic
GTCTTTCTCGATATCATTGG+TGG 0.512811 2_4:-60551130 None:intergenic
TGTGAAGGTGTTGATGAGAA+GGG 0.514263 2_4:+60552069 Msa0339390:CDS
GCCGTGGCACTGAGAAGATT+AGG 0.515227 2_4:+60551253 Msa0339390:CDS
TCAAAATTTGAGAAAATACA+AGG 0.516265 2_4:+60551430 Msa0339390:CDS
TAAGTTGTTGTGAATTGTGA+TGG 0.517874 2_4:+60548659 Msa0339390:exon
GGCCTGGTAAATTGGAGGAC+TGG 0.518791 2_4:+60551617 Msa0339390:CDS
TTGTAAGGAACAACAAGATA+AGG 0.522064 2_4:-60551772 None:intergenic
GAAAGAACTCTTGGAGTCAG+AGG 0.524878 2_4:+60551798 Msa0339390:CDS
TTGTGAAGGTGTTGATGAGA+AGG 0.525741 2_4:+60552068 Msa0339390:CDS
AGAAAGAGGGTGTTGTAGAT+TGG 0.528681 2_4:+60548763 Msa0339390:CDS
TCACTCGAATGATCAGTTGC+AGG 0.531246 2_4:-60551505 None:intergenic
TACGGTCGAGGAGATAATCT+TGG 0.534987 2_4:-60552201 None:intergenic
GACCAGTCCTCCAATTTACC+AGG 0.537067 2_4:-60551619 None:intergenic
ATGGCGATTTGTGGAATCCA+TGG 0.540744 2_4:+60551345 Msa0339390:CDS
ACTTGATCAAATAAAGACAT+TGG 0.542893 2_4:-60551229 None:intergenic
GGCGACTCGCTTGTCACAGT+GGG 0.545011 2_4:+60551280 Msa0339390:CDS
CGGTTGGCCTGGTAAATTGG+AGG 0.545258 2_4:+60551612 Msa0339390:CDS
GCACTGAGAAGATTAGGATC+TGG 0.545953 2_4:+60551259 Msa0339390:CDS
ATGTCACGGAACTTCATGTC+AGG 0.546193 2_4:-60551583 None:intergenic
CAACAAACAAGAATAATCCT+TGG 0.547506 2_4:-60551946 None:intergenic
TACCATCAACTTATTTGTGA+AGG 0.547725 2_4:+60552054 Msa0339390:CDS
GCACAAACCTTAGAGAATGA+TGG 0.548599 2_4:-60548471 None:intergenic
TGTCTCACTACTTGAATGGA+AGG 0.551780 2_4:+60552112 Msa0339390:CDS
TGACCAAGGTTTCTATTTGG+GGG 0.559709 2_4:-60552178 None:intergenic
ACAAGCTGCATTTGGCAACA+AGG 0.560506 2_4:+60551836 Msa0339390:CDS
CTATTAGGCACATACGGTCG+AGG 0.566653 2_4:-60552213 None:intergenic
GAATCATTCGTGGAAATATG+TGG 0.568792 2_4:+60551902 Msa0339390:CDS
TGGAATCCATGGAGGACAGA+GGG 0.574505 2_4:+60551356 Msa0339390:CDS
TCGTGGAAATATGTGGCGGC+CGG 0.577956 2_4:+60551909 Msa0339390:CDS
TCCTAATCTTCTCAGTGCCA+CGG 0.582227 2_4:-60551254 None:intergenic
AGAGGGTGTTGTAGATTGGT+GGG 0.589500 2_4:+60548767 Msa0339390:CDS
TGACAAGGCTTAAGGAGATG+TGG 0.589812 2_4:+60551878 Msa0339390:CDS
GCTGCATTTGGCAACAAGGA+TGG 0.589979 2_4:+60551840 Msa0339390:CDS
TACCTTCTATTAGGCACATA+CGG 0.590743 2_4:-60552219 None:intergenic
TACTTGTGAAACAGTTGAGT+AGG 0.593668 2_4:-60551320 None:intergenic
GAGATGTGGAGAATCATTCG+TGG 0.596543 2_4:+60551892 Msa0339390:CDS
CGGACAAACATCGGTTGCCA+AGG 0.597019 2_4:+60551929 Msa0339390:CDS
AAGAAATTAAAATTATCTGA+AGG 0.600968 2_4:+60551700 Msa0339390:CDS
GTACATATGTTCACTTGTCA+AGG 0.602289 2_4:+60551159 Msa0339390:CDS
AAGTTGTTGTGAATTGTGAT+GGG 0.611718 2_4:+60548660 Msa0339390:exon
AAGTTCCGTGACATAGTCAC+CGG 0.612581 2_4:+60551592 Msa0339390:CDS
TCAATGGCCTTCAACACAAG+AGG 0.613766 2_4:+60551390 Msa0339390:CDS
CAAGGCTTCCCGAATTGCTG+TGG 0.614121 2_4:+60551448 Msa0339390:CDS
TGTGGCGGCCGGACAAACAT+CGG 0.615945 2_4:+60551920 Msa0339390:CDS
GGAGATAATCTTGGTGACCA+AGG 0.616974 2_4:-60552192 None:intergenic
CAACTGTTTCACAAGTAACA+TGG 0.626293 2_4:+60551326 Msa0339390:CDS
TCCGTGACATAGTCACCGGT+TGG 0.633391 2_4:+60551596 Msa0339390:CDS
ATCATAATAATCATGACTCA+GGG 0.639066 2_4:+60552031 Msa0339390:CDS
TTTATTTGATCAAGTAGCCG+TGG 0.639843 2_4:+60551237 Msa0339390:CDS
GCCAACCGGTGACTATGTCA+CGG 0.640919 2_4:-60551597 None:intergenic
TGTTACAACAATAAGAGCAA+TGG 0.641798 2_4:-60548425 None:intergenic
GCATTTGGCAACAAGGATGG+TGG 0.641994 2_4:+60551843 Msa0339390:CDS
TCTGGGTCATCTGAGAAAGA+GGG 0.648304 2_4:+60548750 Msa0339390:CDS
TGATATGCAAGATGAAGTGA+AGG 0.651626 2_4:+60551990 Msa0339390:CDS
TGGCGACTCGCTTGTCACAG+TGG 0.652833 2_4:+60551279 Msa0339390:CDS
GTGGAATCCATGGAGGACAG+AGG 0.657784 2_4:+60551355 Msa0339390:CDS
TCATAGAAACAACCACAGCA+TGG 0.659680 2_4:+60551540 Msa0339390:CDS
CTGATCATTCGAGTGATGTG+TGG 0.665693 2_4:+60551512 Msa0339390:CDS
AGGTGTTGATGAGAAGGGAG+TGG 0.668624 2_4:+60552074 Msa0339390:CDS
TTGCTTGCAAAACCATGCTG+TGG 0.669474 2_4:-60551552 None:intergenic
ATTGTGATGGGTCCTGTGAG+AGG 0.681645 2_4:+60548672 Msa0339390:exon
TGGCACAAAGAGCATTGACA+AGG 0.695151 2_4:+60551863 Msa0339390:CDS
GTTTGTAAAAGTCAAATGCG+CGG 0.698464 2_4:-60551200 None:intergenic
GCGATTTGTGGAATCCATGG+AGG 0.702676 2_4:+60551348 Msa0339390:CDS
TCATTCGTGGAAATATGTGG+CGG 0.720354 2_4:+60551905 Msa0339390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTAAAACAAGAAAT+TGG - chr2_4:60549383-60549402 None:intergenic 10.0%
!! ATCGTAATTAAAATTTAAAT+TGG - chr2_4:60549916-60549935 None:intergenic 10.0%
!! TAAATCATACATTATTATTT+AGG + chr2_4:60550046-60550065 Msa0339390:intron 10.0%
!!! GTATTTTAATAAATTTTGAT+TGG + chr2_4:60550426-60550445 Msa0339390:intron 10.0%
!! TTATTTAGTATGAAAACATT+TGG - chr2_4:60549358-60549377 None:intergenic 15.0%
!!! AAAATTTCTAAAAATGTGAA+TGG - chr2_4:60550687-60550706 None:intergenic 15.0%
!!! TCACATTTTTAGAAATTTTT+TGG + chr2_4:60550688-60550707 Msa0339390:intron 15.0%
!!! CACATTTTTAGAAATTTTTT+GGG + chr2_4:60550689-60550708 Msa0339390:intron 15.0%
!! AAGAAATTAAAATTATCTGA+AGG + chr2_4:60551700-60551719 Msa0339390:CDS 15.0%
!!! TACTAATTTTGCTATTTATA+AGG + chr2_4:60552307-60552326 Msa0339390:three_prime_UTR 15.0%
!! AAAAAAAGGAAAAAACAACA+AGG - chr2_4:60548370-60548389 None:intergenic 20.0%
!! TTGATTAAATATTCCATGAT+TGG - chr2_4:60549027-60549046 None:intergenic 20.0%
!!! TTGTATCATTGAAATTTTTC+AGG + chr2_4:60550125-60550144 Msa0339390:intron 20.0%
!!! TGTATCATTGAAATTTTTCA+GGG + chr2_4:60550126-60550145 Msa0339390:intron 20.0%
!!! TTTTAACTGAATAAACCAAA+AGG + chr2_4:60550532-60550551 Msa0339390:intron 20.0%
!!! AACTTGTTTAATAACCTTTT+AGG + chr2_4:60550656-60550675 Msa0339390:intron 20.0%
!! AAAAGTCAAACAAAAATACA+AGG + chr2_4:60550714-60550733 Msa0339390:intron 20.0%
!!! GGATATAATACAATTTTAGA+AGG + chr2_4:60550880-60550899 Msa0339390:intron 20.0%
!! TCAAAATTTGAGAAAATACA+AGG + chr2_4:60551430-60551449 Msa0339390:CDS 20.0%
!! GAAATAAGGGAATATATAAT+TGG + chr2_4:60551727-60551746 Msa0339390:CDS 20.0%
! ACTATAGTGAGAGAAAAAAA+AGG - chr2_4:60548384-60548403 None:intergenic 25.0%
! AATAATAATGATTCTGCTTC+TGG + chr2_4:60548732-60548751 Msa0339390:CDS 25.0%
! ATAATAATGATTCTGCTTCT+GGG + chr2_4:60548733-60548752 Msa0339390:CDS 25.0%
! GAATTCTCAAAGAAGATAAA+TGG + chr2_4:60548792-60548811 Msa0339390:CDS 25.0%
! TCAACTTGAAAGTCAAATAA+GGG - chr2_4:60548980-60548999 None:intergenic 25.0%
! ATCAACTTGAAAGTCAAATA+AGG - chr2_4:60548981-60549000 None:intergenic 25.0%
!!! TTTTTTGACAGTTATGTTCA+AGG - chr2_4:60549066-60549085 None:intergenic 25.0%
! CAGTGTATGTTAAATTTCTA+TGG + chr2_4:60549246-60549265 Msa0339390:intron 25.0%
!!! GATATACTAGTTCAGTTTTT+TGG + chr2_4:60549511-60549530 Msa0339390:intron 25.0%
!!! TTGCATTTGTTTTATCTTTG+AGG + chr2_4:60549682-60549701 Msa0339390:intron 25.0%
!! TTATCTTTGAGGAATTTAGT+CGG + chr2_4:60549693-60549712 Msa0339390:intron 25.0%
! AATTTATGTTATCAAGTCCA+AGG + chr2_4:60549834-60549853 Msa0339390:intron 25.0%
! ACAACAATGTTGTAAAATCA+AGG - chr2_4:60549987-60550006 None:intergenic 25.0%
!! TTTACAACATTGTTGTTCAT+TGG + chr2_4:60549991-60550010 Msa0339390:intron 25.0%
! TCTACAAAAAAACATCAAGT+TGG - chr2_4:60550169-60550188 None:intergenic 25.0%
!! AAAAAGGTTTTCAGTAAACA+TGG - chr2_4:60550281-60550300 None:intergenic 25.0%
! TCAAGTAATTCTACTGAAAA+AGG - chr2_4:60550297-60550316 None:intergenic 25.0%
! AAGATTTGAAAACTGAATAC+TGG - chr2_4:60550357-60550376 None:intergenic 25.0%
!! TTGTAAGTTGTTAAACCTTT+TGG - chr2_4:60550550-60550569 None:intergenic 25.0%
! TAACATAAACCTGCATAAAA+TGG - chr2_4:60550745-60550764 None:intergenic 25.0%
! CTCACATATTTAGTAATCTT+TGG + chr2_4:60550778-60550797 Msa0339390:intron 25.0%
! ACTTGATCAAATAAAGACAT+TGG - chr2_4:60551232-60551251 None:intergenic 25.0%
! TTCAACAAACTTTGTGATAA+AGG + chr2_4:60551661-60551680 Msa0339390:CDS 25.0%
!! GAATATATAATTGGTGATTC+AGG + chr2_4:60551736-60551755 Msa0339390:CDS 25.0%
! TATCATAATAATCATGACTC+AGG + chr2_4:60552030-60552049 Msa0339390:CDS 25.0%
! ATCATAATAATCATGACTCA+GGG + chr2_4:60552031-60552050 Msa0339390:CDS 25.0%
! TGACTAAGTAAAAGAAATCA+TGG - chr2_4:60552144-60552163 None:intergenic 25.0%
!!! TATGTTGTTTTTAGATGTGA+AGG + chr2_4:60552251-60552270 Msa0339390:three_prime_UTR 25.0%
! AGGTTTGAACATATTTAGTT+AGG + chr2_4:60552327-60552346 Msa0339390:three_prime_UTR 25.0%
! GGTTTGAACATATTTAGTTA+GGG + chr2_4:60552328-60552347 Msa0339390:three_prime_UTR 25.0%
! ACTTGAAGTAATTTACTAGA+AGG + chr2_4:60552383-60552402 Msa0339390:three_prime_UTR 25.0%
AGGAAAAAACAACAAGGTTA+AGG - chr2_4:60548364-60548383 None:intergenic 30.0%
TGTTACAACAATAAGAGCAA+TGG - chr2_4:60548428-60548447 None:intergenic 30.0%
TCTGCAAGAAAGAATTCAAA+GGG - chr2_4:60548607-60548626 None:intergenic 30.0%
ATCTGCAAGAAAGAATTCAA+AGG - chr2_4:60548608-60548627 None:intergenic 30.0%
!!! TTGAATCAGAAGTTTTGAAG+CGG - chr2_4:60548638-60548657 None:intergenic 30.0%
TAAGTTGTTGTGAATTGTGA+TGG + chr2_4:60548659-60548678 Msa0339390:exon 30.0%
AAGTTGTTGTGAATTGTGAT+GGG + chr2_4:60548660-60548679 Msa0339390:exon 30.0%
!!! GATTTTTTGCTGATGAATCA+TGG + chr2_4:60548893-60548912 Msa0339390:intron 30.0%
CAACTTGAAAGTCAAATAAG+GGG - chr2_4:60548979-60548998 None:intergenic 30.0%
ATGTGTTATCTTACCAATCA+TGG + chr2_4:60549011-60549030 Msa0339390:intron 30.0%
GAGTTTAAGAATGAGGTTTA+GGG - chr2_4:60549164-60549183 None:intergenic 30.0%
GTAACTTAAGTGGTTAATTC+AGG - chr2_4:60549197-60549216 None:intergenic 30.0%
!! TGTGATTTCTGGTTTATATG+AGG + chr2_4:60549302-60549321 Msa0339390:intron 30.0%
TTGAGCAATACAGAATATGT+AGG - chr2_4:60549552-60549571 None:intergenic 30.0%
TTATCAAGTCCAAGGAAAAA+AGG + chr2_4:60549842-60549861 Msa0339390:intron 30.0%
AAAGCAAATTACTTGCAAAC+TGG - chr2_4:60550500-60550519 None:intergenic 30.0%
TAACCTATAAGCATGTATAG+CGG - chr2_4:60550589-60550608 None:intergenic 30.0%
AACAAATTGTACTACTGTGT+AGG + chr2_4:60550614-60550633 Msa0339390:intron 30.0%
! AAGGTAATACCATTTTATGC+AGG + chr2_4:60550733-60550752 Msa0339390:intron 30.0%
!! TCAAGGATCATGTTATTTCT+AGG + chr2_4:60550847-60550866 Msa0339390:intron 30.0%
!! ATCATGTTATTTCTAGGAAC+AGG + chr2_4:60550853-60550872 Msa0339390:intron 30.0%
!! TTATTTCTAGGAACAGGAAT+TGG + chr2_4:60550859-60550878 Msa0339390:intron 30.0%
GATAAAGGAGAAAGACTAAA+TGG + chr2_4:60551676-60551695 Msa0339390:CDS 30.0%
TATCTGAAGGATCAGAAATA+AGG + chr2_4:60551713-60551732 Msa0339390:CDS 30.0%
ATCTGAAGGATCAGAAATAA+GGG + chr2_4:60551714-60551733 Msa0339390:CDS 30.0%
TTGTAAGGAACAACAAGATA+AGG - chr2_4:60551775-60551794 None:intergenic 30.0%
AAGAGTTCTTTCTCTTTGTA+AGG - chr2_4:60551790-60551809 None:intergenic 30.0%
TTACAAAGAGAAAGAACTCT+TGG + chr2_4:60551789-60551808 Msa0339390:CDS 30.0%
!! TCATTTTAACAAGCTGCATT+TGG + chr2_4:60551828-60551847 Msa0339390:CDS 30.0%
CAACAAACAAGAATAATCCT+TGG - chr2_4:60551949-60551968 None:intergenic 30.0%
TACCATCAACTTATTTGTGA+AGG + chr2_4:60552054-60552073 Msa0339390:CDS 30.0%
TAATCCGTATACCTTCTATT+AGG - chr2_4:60552231-60552250 None:intergenic 30.0%
ACAACAAGGTTAAGGTTATG+AGG - chr2_4:60548356-60548375 None:intergenic 35.0%
CTAATGTCCATCATTCTCTA+AGG + chr2_4:60548464-60548483 Msa0339390:five_prime_UTR 35.0%
!! GTTTGCTATGATGAATGTGT+TGG + chr2_4:60548915-60548934 Msa0339390:intron 35.0%
GGAGTTTAAGAATGAGGTTT+AGG - chr2_4:60549165-60549184 None:intergenic 35.0%
!!! GGAGATAGAAGCTTTTTTCT+TGG + chr2_4:60549267-60549286 Msa0339390:intron 35.0%
TGTAATCTGTCTGTGATTTC+TGG + chr2_4:60549291-60549310 Msa0339390:intron 35.0%
CATATTCTGTATTGCTCAAG+TGG + chr2_4:60549553-60549572 Msa0339390:intron 35.0%
CAAGTGGTTAATTTAAGCCT+AGG + chr2_4:60549569-60549588 Msa0339390:intron 35.0%
GCAATTAGTATGCTTGTAGA+AGG + chr2_4:60549610-60549629 Msa0339390:intron 35.0%
TTGTTGGACGAATCAAAATG+AGG + chr2_4:60549725-60549744 Msa0339390:intron 35.0%
AATTATCACATCATGTCCTG+TGG + chr2_4:60549759-60549778 Msa0339390:intron 35.0%
ATGAATTCTATTTCCTCCAC+AGG - chr2_4:60549778-60549797 None:intergenic 35.0%
CATGATGATCATATGTCACA+AGG - chr2_4:60550225-60550244 None:intergenic 35.0%
CTTGTGACATATGATCATCA+TGG + chr2_4:60550223-60550242 Msa0339390:intron 35.0%
AATTGTACTACTGTGTAGGT+TGG + chr2_4:60550618-60550637 Msa0339390:intron 35.0%
TGTGTGATCATTAGTGATGT+CGG + chr2_4:60550812-60550831 Msa0339390:intron 35.0%
!! AACACAAGTTCTAAGGTTTG+AGG - chr2_4:60551087-60551106 None:intergenic 35.0%
! ACCTTAGAACTTGTGTTGAT+TGG + chr2_4:60551090-60551109 Msa0339390:intron 35.0%
AACGTCTTTCTCGATATCAT+TGG - chr2_4:60551136-60551155 None:intergenic 35.0%
GTACATATGTTCACTTGTCA+AGG + chr2_4:60551159-60551178 Msa0339390:CDS 35.0%
GTTTGTAAAAGTCAAATGCG+CGG - chr2_4:60551203-60551222 None:intergenic 35.0%
!! GCGCATTTGACTTTTACAAA+CGG + chr2_4:60551202-60551221 Msa0339390:CDS 35.0%
TTTATTTGATCAAGTAGCCG+TGG + chr2_4:60551237-60551256 Msa0339390:CDS 35.0%
TACTTGTGAAACAGTTGAGT+AGG - chr2_4:60551323-60551342 None:intergenic 35.0%
CAACTGTTTCACAAGTAACA+TGG + chr2_4:60551326-60551345 Msa0339390:CDS 35.0%
TTCATTTCCTCTTGTGTTGA+AGG - chr2_4:60551400-60551419 None:intergenic 35.0%
ACAACAAGATAAGGCAATAG+AGG - chr2_4:60551766-60551785 None:intergenic 35.0%
GAATCATTCGTGGAAATATG+TGG + chr2_4:60551902-60551921 Msa0339390:CDS 35.0%
TGATATGCAAGATGAAGTGA+AGG + chr2_4:60551990-60552009 Msa0339390:CDS 35.0%
! CACCTTCACAAATAAGTTGA+TGG - chr2_4:60552059-60552078 None:intergenic 35.0%
AGTTTGTCTCACTACTTGAA+TGG + chr2_4:60552108-60552127 Msa0339390:CDS 35.0%
TACCTTCTATTAGGCACATA+CGG - chr2_4:60552222-60552241 None:intergenic 35.0%
! TGTGCCTAATAGAAGGTATA+CGG + chr2_4:60552224-60552243 Msa0339390:three_prime_UTR 35.0%
AGGTGAGAAAAACACAATAG+CGG + chr2_4:60552271-60552290 Msa0339390:three_prime_UTR 35.0%
GCAATGGTAGCATGCAAATA+AGG - chr2_4:60548412-60548431 None:intergenic 40.0%
GCACAAACCTTAGAGAATGA+TGG - chr2_4:60548474-60548493 None:intergenic 40.0%
!! CTCTTTTTGATTCCTCTCAC+AGG - chr2_4:60548687-60548706 None:intergenic 40.0%
!! AGAAAGAGGGTGTTGTAGAT+TGG + chr2_4:60548763-60548782 Msa0339390:CDS 40.0%
ACCTCATTCTTAAACTCCTG+CGG + chr2_4:60549167-60549186 Msa0339390:intron 40.0%
TAACCACTTAAGTTACGCCT+TGG + chr2_4:60549201-60549220 Msa0339390:intron 40.0%
AACCACTTAAGTTACGCCTT+GGG + chr2_4:60549202-60549221 Msa0339390:intron 40.0%
AATGTACTATGTAGTCCCCA+AGG - chr2_4:60549221-60549240 None:intergenic 40.0%
GCATACTAATTGCTACCATC+AGG - chr2_4:60549603-60549622 None:intergenic 40.0%
! TTGATTCGTCCAACAAAACC+AGG - chr2_4:60549721-60549740 None:intergenic 40.0%
!!! TTGACTGCACCTTTTTTCCT+TGG - chr2_4:60549854-60549873 None:intergenic 40.0%
GACAAATCGACAATGAACCA+AGG + chr2_4:60550074-60550093 Msa0339390:intron 40.0%
GAACCAAGGCTTTCAATTCT+TGG + chr2_4:60550088-60550107 Msa0339390:intron 40.0%
GAACCAAGAATTGAAAGCCT+TGG - chr2_4:60550094-60550113 None:intergenic 40.0%
GTGACATATGATCATCATGG+TGG + chr2_4:60550226-60550245 Msa0339390:intron 40.0%
! CATGGTCTGGTTTCAGTTAA+GGG - chr2_4:60550263-60550282 None:intergenic 40.0%
! ACATGGTCTGGTTTCAGTTA+AGG - chr2_4:60550264-60550283 None:intergenic 40.0%
! GGTTTTCAGTAAACATGGTC+TGG - chr2_4:60550276-60550295 None:intergenic 40.0%
! TGTGAATGGCTAGTCCTAAA+AGG - chr2_4:60550673-60550692 None:intergenic 40.0%
TGATCATTAGTGATGTCGGT+TGG + chr2_4:60550816-60550835 Msa0339390:intron 40.0%
!! GTCGGTTGGTACATTTTTCA+AGG + chr2_4:60550830-60550849 Msa0339390:intron 40.0%
TGTGAGAAAACCTCATCTGA+CGG - chr2_4:60550906-60550925 None:intergenic 40.0%
GCCAATCAACACAAGTTCTA+AGG - chr2_4:60551094-60551113 None:intergenic 40.0%
!! TAGAACTTGTGTTGATTGGC+AGG + chr2_4:60551094-60551113 Msa0339390:intron 40.0%
GTCTTTCTCGATATCATTGG+TGG - chr2_4:60551133-60551152 None:intergenic 40.0%
ACAAGTAACATGGCGATTTG+TGG + chr2_4:60551336-60551355 Msa0339390:CDS 40.0%
TCATAGAAACAACCACAGCA+TGG + chr2_4:60551540-60551559 Msa0339390:CDS 40.0%
!! AAGAGCATTGACAAGGCTTA+AGG + chr2_4:60551870-60551889 Msa0339390:CDS 40.0%
TCATTCGTGGAAATATGTGG+CGG + chr2_4:60551905-60551924 Msa0339390:CDS 40.0%
!! TTGTGAAGGTGTTGATGAGA+AGG + chr2_4:60552068-60552087 Msa0339390:CDS 40.0%
!! TGTGAAGGTGTTGATGAGAA+GGG + chr2_4:60552069-60552088 Msa0339390:CDS 40.0%
TGTCTCACTACTTGAATGGA+AGG + chr2_4:60552112-60552131 Msa0339390:CDS 40.0%
TGACCAAGGTTTCTATTTGG+GGG - chr2_4:60552181-60552200 None:intergenic 40.0%
GTGACCAAGGTTTCTATTTG+GGG - chr2_4:60552182-60552201 None:intergenic 40.0%
GGTGACCAAGGTTTCTATTT+GGG - chr2_4:60552183-60552202 None:intergenic 40.0%
TGGTGACCAAGGTTTCTATT+TGG - chr2_4:60552184-60552203 None:intergenic 40.0%
GGTGAGAAAAACACAATAGC+GGG + chr2_4:60552272-60552291 Msa0339390:three_prime_UTR 40.0%
GTGAGAAAAACACAATAGCG+GGG + chr2_4:60552273-60552292 Msa0339390:three_prime_UTR 40.0%
TTCTGGGTCATCTGAGAAAG+AGG + chr2_4:60548749-60548768 Msa0339390:CDS 45.0%
TCTGGGTCATCTGAGAAAGA+GGG + chr2_4:60548750-60548769 Msa0339390:CDS 45.0%
!! AAGAGGGTGTTGTAGATTGG+TGG + chr2_4:60548766-60548785 Msa0339390:CDS 45.0%
!! AGAGGGTGTTGTAGATTGGT+GGG + chr2_4:60548767-60548786 Msa0339390:CDS 45.0%
CCTCATTCTTAAACTCCTGC+GGG + chr2_4:60549168-60549187 Msa0339390:intron 45.0%
ACCACTTAAGTTACGCCTTG+GGG + chr2_4:60549203-60549222 Msa0339390:intron 45.0%
ACCATCAGGCTGATATACCT+AGG - chr2_4:60549589-60549608 None:intergenic 45.0%
! TGAGGAATTTAGTCGGTTCC+TGG + chr2_4:60549700-60549719 Msa0339390:intron 45.0%
!!! TAGTCGGTTCCTGGTTTTGT+TGG + chr2_4:60549709-60549728 Msa0339390:intron 45.0%
TATCACATCATGTCCTGTGG+AGG + chr2_4:60549762-60549781 Msa0339390:intron 45.0%
CAGCCGCTATACATGCTTAT+AGG + chr2_4:60550583-60550602 Msa0339390:intron 45.0%
!! TTTTAGAAGGCCGTCAGATG+AGG + chr2_4:60550893-60550912 Msa0339390:intron 45.0%
GTTATGCATTGCTGCAACTC+AGG + chr2_4:60550968-60550987 Msa0339390:intron 45.0%
TCCTAATCTTCTCAGTGCCA+CGG - chr2_4:60551257-60551276 None:intergenic 45.0%
GCACTGAGAAGATTAGGATC+TGG + chr2_4:60551259-60551278 Msa0339390:CDS 45.0%
TCACAGTGGGCGATTCATTT+GGG + chr2_4:60551293-60551312 Msa0339390:CDS 45.0%
ATGGCGATTTGTGGAATCCA+TGG + chr2_4:60551345-60551364 Msa0339390:CDS 45.0%
CTTGTGTTGAAGGCCATTGA+AGG - chr2_4:60551390-60551409 None:intergenic 45.0%
TCAATGGCCTTCAACACAAG+AGG + chr2_4:60551390-60551409 Msa0339390:CDS 45.0%
TCACTCGAATGATCAGTTGC+AGG - chr2_4:60551508-60551527 None:intergenic 45.0%
CTGATCATTCGAGTGATGTG+TGG + chr2_4:60551512-60551531 Msa0339390:CDS 45.0%
TTGCTTGCAAAACCATGCTG+TGG - chr2_4:60551555-60551574 None:intergenic 45.0%
ATGTCACGGAACTTCATGTC+AGG - chr2_4:60551586-60551605 None:intergenic 45.0%
AAGTTCCGTGACATAGTCAC+CGG + chr2_4:60551592-60551611 Msa0339390:CDS 45.0%
GAAAGAACTCTTGGAGTCAG+AGG + chr2_4:60551798-60551817 Msa0339390:CDS 45.0%
ACAAGCTGCATTTGGCAACA+AGG + chr2_4:60551836-60551855 Msa0339390:CDS 45.0%
!! TGGCACAAAGAGCATTGACA+AGG + chr2_4:60551863-60551882 Msa0339390:CDS 45.0%
TGACAAGGCTTAAGGAGATG+TGG + chr2_4:60551878-60551897 Msa0339390:CDS 45.0%
GAGATGTGGAGAATCATTCG+TGG + chr2_4:60551892-60551911 Msa0339390:CDS 45.0%
TAGCCCCCAAATAGAAACCT+TGG + chr2_4:60552175-60552194 Msa0339390:three_prime_UTR 45.0%
! GGAGATAATCTTGGTGACCA+AGG - chr2_4:60552195-60552214 None:intergenic 45.0%
TACGGTCGAGGAGATAATCT+TGG - chr2_4:60552204-60552223 None:intergenic 45.0%
GACCGTATGTGCCTAATAGA+AGG + chr2_4:60552217-60552236 Msa0339390:three_prime_UTR 45.0%
!! ATTGTGATGGGTCCTGTGAG+AGG + chr2_4:60548672-60548691 Msa0339390:exon 50.0%
CCCGCAGGAGTTTAAGAATG+AGG - chr2_4:60549171-60549190 None:intergenic 50.0%
GGTTAATTCAGGATACCCGC+AGG - chr2_4:60549186-60549205 None:intergenic 50.0%
TCCCCAAGGCGTAACTTAAG+TGG - chr2_4:60549207-60549226 None:intergenic 50.0%
GCCTAGGTATATCAGCCTGA+TGG + chr2_4:60549585-60549604 Msa0339390:intron 50.0%
GTCACAGTGGGCGATTCATT+TGG + chr2_4:60551292-60551311 Msa0339390:CDS 50.0%
GCGATTTGTGGAATCCATGG+AGG + chr2_4:60551348-60551367 Msa0339390:CDS 50.0%
TGGAATCCATGGAGGACAGA+GGG + chr2_4:60551356-60551375 Msa0339390:CDS 50.0%
GAGGGCTTCATCACCTTCAA+TGG + chr2_4:60551374-60551393 Msa0339390:CDS 50.0%
TCAACAGCGCCACAGCAATT+CGG - chr2_4:60551460-60551479 None:intergenic 50.0%
CTCCAATTTACCAGGCCAAC+CGG - chr2_4:60551614-60551633 None:intergenic 50.0%
GACCAGTCCTCCAATTTACC+AGG - chr2_4:60551622-60551641 None:intergenic 50.0%
GCTGCATTTGGCAACAAGGA+TGG + chr2_4:60551840-60551859 Msa0339390:CDS 50.0%
GCATTTGGCAACAAGGATGG+TGG + chr2_4:60551843-60551862 Msa0339390:CDS 50.0%
CTTGGCAACCGATGTTTGTC+CGG - chr2_4:60551931-60551950 None:intergenic 50.0%
!! AGGTGTTGATGAGAAGGGAG+TGG + chr2_4:60552074-60552093 Msa0339390:CDS 50.0%
CTATTAGGCACATACGGTCG+AGG - chr2_4:60552216-60552235 None:intergenic 50.0%
! GCCGTGGCACTGAGAAGATT+AGG + chr2_4:60551253-60551272 Msa0339390:CDS 55.0%
GTGGAATCCATGGAGGACAG+AGG + chr2_4:60551355-60551374 Msa0339390:CDS 55.0%
ATGAAGCCCTCTGTCCTCCA+TGG - chr2_4:60551365-60551384 None:intergenic 55.0%
CAAGGCTTCCCGAATTGCTG+TGG + chr2_4:60551448-60551467 Msa0339390:CDS 55.0%
CAACAGCGCCACAGCAATTC+GGG - chr2_4:60551459-60551478 None:intergenic 55.0%
TCCGTGACATAGTCACCGGT+TGG + chr2_4:60551596-60551615 Msa0339390:CDS 55.0%
GCCAACCGGTGACTATGTCA+CGG - chr2_4:60551600-60551619 None:intergenic 55.0%
!! CACCGGTTGGCCTGGTAAAT+TGG + chr2_4:60551609-60551628 Msa0339390:CDS 55.0%
!! CGGTTGGCCTGGTAAATTGG+AGG + chr2_4:60551612-60551631 Msa0339390:CDS 55.0%
GGCCTGGTAAATTGGAGGAC+TGG + chr2_4:60551617-60551636 Msa0339390:CDS 55.0%
TCGTGGAAATATGTGGCGGC+CGG + chr2_4:60551909-60551928 Msa0339390:CDS 55.0%
! CGGACAAACATCGGTTGCCA+AGG + chr2_4:60551929-60551948 Msa0339390:CDS 55.0%
TGGCGACTCGCTTGTCACAG+TGG + chr2_4:60551279-60551298 Msa0339390:CDS 60.0%
GGCGACTCGCTTGTCACAGT+GGG + chr2_4:60551280-60551299 Msa0339390:CDS 60.0%
! GACATAGTCACCGGTTGGCC+TGG + chr2_4:60551601-60551620 Msa0339390:CDS 60.0%
TGTGGCGGCCGGACAAACAT+CGG + chr2_4:60551920-60551939 Msa0339390:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 60548307 60552426 60548307 ID=Msa0339390;Name=Msa0339390
chr2_4 mRNA 60548307 60552426 60548307 ID=Msa0339390-mRNA-1;Parent=Msa0339390;Name=Msa0339390-mRNA-1;_AED=0.24;_eAED=0.24;_QI=231|1|1|1|0.5|0.33|3|280|388
chr2_4 exon 60548307 60548485 60548307 ID=Msa0339390-mRNA-1:exon:18811;Parent=Msa0339390-mRNA-1
chr2_4 exon 60548626 60548813 60548626 ID=Msa0339390-mRNA-1:exon:18812;Parent=Msa0339390-mRNA-1
chr2_4 exon 60551116 60552426 60551116 ID=Msa0339390-mRNA-1:exon:18813;Parent=Msa0339390-mRNA-1
chr2_4 five_prime_UTR 60548307 60548485 60548307 ID=Msa0339390-mRNA-1:five_prime_utr;Parent=Msa0339390-mRNA-1
chr2_4 five_prime_UTR 60548626 60548677 60548626 ID=Msa0339390-mRNA-1:five_prime_utr;Parent=Msa0339390-mRNA-1
chr2_4 CDS 60548678 60548813 60548678 ID=Msa0339390-mRNA-1:cds;Parent=Msa0339390-mRNA-1
chr2_4 CDS 60551116 60552146 60551116 ID=Msa0339390-mRNA-1:cds;Parent=Msa0339390-mRNA-1
chr2_4 three_prime_UTR 60552147 60552426 60552147 ID=Msa0339390-mRNA-1:three_prime_utr;Parent=Msa0339390-mRNA-1
Gene Sequence

>Msa0339390

ATGGGTCCTGTGAGAGGAATCAAAAAGAGAAGAAAGACAGAGAAGAGTTATGACAATAATAATGATTCTGCTTCTGGGTCATCTGAGAAAGAGGGTGTTGTAGATTGGTGGGATGAATTCTCAAAGAAGATAAATGGTCTTCAGAAGTCACCACCAATGATATCGAGAAAGACGTTTGAGTACATATGTTCACTTGTCAAGGACGATATGACAACAAAATCCGCGCATTTGACTTTTACAAACGGAAAGCCAATGTCTTTATTTGATCAAGTAGCCGTGGCACTGAGAAGATTAGGATCTGGCGACTCGCTTGTCACAGTGGGCGATTCATTTGGGCTAACCTACTCAACTGTTTCACAAGTAACATGGCGATTTGTGGAATCCATGGAGGACAGAGGGCTTCATCACCTTCAATGGCCTTCAACACAAGAGGAAATGAATGCAATCAAGTCAAAATTTGAGAAAATACAAGGCTTCCCGAATTGCTGTGGCGCTGTTGATGCTACTCACATCACAATGTGTTTACCTGCAACTGATCATTCGAGTGATGTGTGGCTTGATCATAGAAACAACCACAGCATGGTTTTGCAAGCAATAGTTGATCCTGACATGAAGTTCCGTGACATAGTCACCGGTTGGCCTGGTAAATTGGAGGACTGGTCAATATTCGAGAGTTCAAATTTCAACAAACTTTGTGATAAAGGAGAAAGACTAAATGGAAAGAAATTAAAATTATCTGAAGGATCAGAAATAAGGGAATATATAATTGGTGATTCAGGTTATCCTCTATTGCCTTATCTTGTTGTTCCTTACAAAGAGAAAGAACTCTTGGAGTCAGAGGCAAAAACTCATTTTAACAAGCTGCATTTGGCAACAAGGATGGTGGCACAAAGAGCATTGACAAGGCTTAAGGAGATGTGGAGAATCATTCGTGGAAATATGTGGCGGCCGGACAAACATCGGTTGCCAAGGATTATTCTTGTTTGTTGCTTACTTCACAACATTGTCATTGATATGCAAGATGAAGTGAAGGATGAATTGCTCTATTTGTATCATAATAATCATGACTCAGGGTACCATCAACTTATTTGTGAAGGTGTTGATGAGAAGGGAGTGGCACTAAGAGAAAGTTTGTCTCACTACTTGAATGGAAGGTTGCATCCATGA
Protein sequence

>Msa0339390

MGPVRGIKKRRKTEKSYDNNNDSASGSSEKEGVVDWWDEFSKKINGLQKSPPMISRKTFEYICSLVKDDMTTKSAHLTFTNGKPMSLFDQVAVALRRLGSGDSLVTVGDSFGLTYSTVSQVTWRFVESMEDRGLHHLQWPSTQEEMNAIKSKFEKIQGFPNCCGAVDATHITMCLPATDHSSDVWLDHRNNHSMVLQAIVDPDMKFRDIVTGWPGKLEDWSIFESSNFNKLCDKGERLNGKKLKLSEGSEIREYIIGDSGYPLLPYLVVPYKEKELLESEAKTHFNKLHLATRMVAQRALTRLKEMWRIIRGNMWRPDKHRLPRIILVCCLLHNIVIDMQDEVKDELLYLYHNNHDSGYHQLICEGVDEKGVALRESLSHYLNGRLHP*