AlfalfaGEDB | Msa0224160

M. sativa cultivar zhongmu-4 / Msa0224160

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009719 GO:0009725 GO:0009739 GO:0009832 GO:0009834 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044085 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071554 GO:0071669 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224160	XP_003597573.1	93.052	403	19	4	1	396	1	401	0.0	701

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224160	sp\|Q9SPG3\|MYB26_ARATH	44.759	353	161	10	1	324	1	348	1.84e-71	231

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224160	G7IS44	93.052	403	19	4	1	396	1	401	0.0	701

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TFs/TRs:

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Gene ID	Type	Classification
Msa0224160	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0224160	Msa0265300	0.916697	1.303543e-85	-8.615850e-47
Msa0224160	Msa1364050	0.852759	3.737880e-61	-8.615850e-47
Msa0224160	Msa1452990	0.837292	5.666436e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224160	MtrunA17_Chr2g0330041	93.052	403	19	4	1	396	1	401	0.0	701
Msa0224160	MtrunA17_Chr8g0342481	57.704	331	103	16	1	319	1	306	9.46e-98	297
Msa0224160	MtrunA17_Chr8g0342541	58.675	317	100	16	1	312	1	291	1.83e-97	294
Msa0224160	MtrunA17_Chr8g0342521	53.659	369	122	20	1	366	1	323	9.87e-94	285
Msa0224160	MtrunA17_Chr1g0193371	72.180	133	37	0	1	133	1	133	5.76e-69	222
Msa0224160	MtrunA17_Chr7g0270891	71.429	133	38	0	1	133	1	133	4.16e-68	219
Msa0224160	MtrunA17_Chr2g0309911	73.529	136	35	1	1	136	1	135	5.07e-68	218
Msa0224160	MtrunA17_Chr4g0058631	76.800	125	29	0	1	125	1	125	1.03e-67	218
Msa0224160	MtrunA17_Chr3g0117091	76.000	125	30	0	1	125	1	125	1.69e-66	215
Msa0224160	MtrunA17_Chr1g0193801	69.173	133	41	0	1	133	1	133	1.83e-66	216

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224160	AT3G13890.2	45.930	344	161	9	1	324	1	339	1.55e-75	239
Msa0224160	AT3G13890.1	44.759	353	161	10	1	324	1	348	1.87e-72	231
Msa0224160	AT5G26660.1	77.600	125	28	0	1	125	1	125	2.27e-69	223
Msa0224160	AT1G09540.1	70.922	141	35	1	1	135	1	141	2.58e-69	223
Msa0224160	AT1G57560.1	69.065	139	43	0	1	139	1	139	1.96e-68	219
Msa0224160	AT4G01680.3	71.970	132	37	0	1	132	1	132	4.51e-68	216
Msa0224160	AT1G57560.2	74.400	125	32	0	1	125	1	125	7.43e-68	216
Msa0224160	AT4G01680.1	71.970	132	37	0	1	132	1	132	3.67e-67	216
Msa0224160	AT3G12720.1	77.600	125	27	1	1	125	12	135	4.61e-67	215
Msa0224160	AT1G63910.1	79.310	116	24	0	1	116	1	116	1.94e-66	216

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Find 67 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCCACGTTTCAAATC+AGG	0.335974	2_1:+73932825	None:intergenic
AAGAAGCAGAAGAAGAAAAT+GGG	0.436899	2_1:+73932000	None:intergenic
AATAAACAGAAGGTGAAAAG+AGG	0.582306	2_1:-73932990	Msa0224160:CDS
ACAAACATAATTATCCTTAT+TGG	0.182467	2_1:+73932028	None:intergenic
ACAGGATTGTCATTAAATAA+AGG	0.316668	2_1:+73932467	None:intergenic
ACAGTGAATATCAGAAAATG+TGG	0.592062	2_1:+73932520	None:intergenic
ACCAAAGAATGATCATCAAG+TGG	0.657833	2_1:-73931847	Msa0224160:CDS
ACCAGGAAGAACAGATAATG+AGG	0.592167	2_1:-73932612	Msa0224160:CDS
ACCTCATTATCTGTTCTTCC+TGG	0.311501	2_1:+73932611	None:intergenic
ACTGGTTGATAGAATGATAG+TGG	0.574540	2_1:+73931759	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAAACAGAAGGTGAAAAG+AGG	-	chr2_1:73931752-73931771	Msa0224160:CDS	30.0%
TACATCACTAATTATGGACA+TGG	-	chr2_1:73931812-73931831	Msa0224160:CDS	30.0%
ATTGAACTTCATACCATTCT+AGG	-	chr2_1:73931965-73931984	Msa0224160:CDS	30.0%
ACAGTGAATATCAGAAAATG+TGG	+	chr2_1:73932225-73932244	None:intergenic	30.0%
TTCTGAGCATGTAAGATATT+GGG	+	chr2_1:73932425-73932444	None:intergenic	30.0%
ATTCTGAGCATGTAAGATAT+TGG	+	chr2_1:73932426-73932445	None:intergenic	30.0%
TATGAAGATGGGATATTTGA+TGG	+	chr2_1:73932476-73932495	None:intergenic	30.0%
AAGAAGCAGAAGAAGAAAAT+GGG	+	chr2_1:73932745-73932764	None:intergenic	30.0%
AATAAACAGAAGGTGAAAAG+AGG	-	chr2_1:73931752-73931771	Msa0224160:CDS	30.0%
TACATCACTAATTATGGACA+TGG	-	chr2_1:73931812-73931831	Msa0224160:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	73931731	73933033	73931731	ID=Msa0224160;Name=Msa0224160
chr2_1	mRNA	73931731	73933033	73931731	ID=Msa0224160-mRNA-1;Parent=Msa0224160;Name=Msa0224160-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|1\|1\|1\|2\|0\|396
chr2_1	exon	73932771	73933033	73932771	ID=Msa0224160-mRNA-1:exon:21801;Parent=Msa0224160-mRNA-1
chr2_1	exon	73931731	73932658	73931731	ID=Msa0224160-mRNA-1:exon:21800;Parent=Msa0224160-mRNA-1
chr2_1	CDS	73932771	73933033	73932771	ID=Msa0224160-mRNA-1:cds;Parent=Msa0224160-mRNA-1
chr2_1	CDS	73931731	73932658	73931731	ID=Msa0224160-mRNA-1:cds;Parent=Msa0224160-mRNA-1

Gene Sequence

>Msa0224160

ATGGGGCATCACTCTTGCTGCAATAAACAGAAGGTGAAAAGAGGACTATGGTCACCGGAAGAAGATGAAAAACTCATCAACTACATCACTAATTATGGACATGGTTGTTGGAGCTCTGTCCCTAAACTTGCAGGACTGCAGAGATGTGGAAAAAGTTGCAGGTTGAGATGGATAAATTATTTAAGACCTGATTTGAAACGTGGAAGTTTTTCTCCACAAGAAGCTGCTCTCATTATTGAACTTCATACCATTCTAGGCAACAGGTGGGCTCAGATAGCAAAGCATTTACCAGGAAGAACAGATAATGAGGTGAAGAACTTTTGGAATTCAAGCATAAAGAAGAAACTACTTTCACATGATTTTGTTCCCACTTCTTTAGCCACATTTTCTGATATTCACTGTCCTAGAAATGGTTCTGTTGAAAGTTTCTTCCCTTTATTTAATGACAATCCTGTCCTTAACTCCAATCATCAATATCACTTTGACAACCACCTATACCTTCCCATCAATATCCCAACTCCAATCCTACAAGGTATTGATCAAAATGATATCAAAAATATTGATATCAACGGTTATAATCCCAATATCTTACATGCTCAGAATAATCCAATATTGCCTGAAACAGTACCACCATCAAATATCCCATCTTCATATGAATTAGATACATGGTCATTAATTCATCATCTCAATCCAAATATTCAACAAAATCATCAAATGACCACAAAAAGTGATGCTGCCTCCCAAAACTATAATATTGTTGATCAGAGTTTTATCAATATTCCAAATACATGGCAGCAGCAGCACTATGATAGTAATAGTCATTTAGTGAATCAACTTGAACCTATTGTGCAAAAAGTCTTTGAAAGTGAAACCAATAAGGATAATTATGTTTGTAGCATCCCATTTTCTTCTTCTGCTTCTTCACAAGAACATGATCATCATCATCATCACCATGATGTTGTCAACCAAATTGTTGATCAGTGTTATAATACAGATCATTATCAAGGAACTATAATTTGTCCAAAACAAGATCATCATCAAGTTGTTGCTGCACCAAAGAATGATCATCAAGTGGAGTATAACATTGAAGCTCTCATCATGTCATCATTGCCATCCTCTACAACAACATCTTCTTCATCACCACTATCATTCTATCAACCAGTCACTCTCACAAAACCTATCCTTCCTTAA

Protein sequence

>Msa0224160

MGHHSCCNKQKVKRGLWSPEEDEKLINYITNYGHGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGSFSPQEAALIIELHTILGNRWAQIAKHLPGRTDNEVKNFWNSSIKKKLLSHDFVPTSLATFSDIHCPRNGSVESFFPLFNDNPVLNSNHQYHFDNHLYLPINIPTPILQGIDQNDIKNIDINGYNPNILHAQNNPILPETVPPSNIPSSYELDTWSLIHHLNPNIQQNHQMTTKSDAASQNYNIVDQSFINIPNTWQQQHYDSNSHLVNQLEPIVQKVFESETNKDNYVCSIPFSSSASSQEHDHHHHHHDVVNQIVDQCYNTDHYQGTIICPKQDHHQVVAAPKNDHQVEYNIEALIMSSLPSSTTTSSSSPLSFYQPVTLTKPILP*