AlfalfaGEDB | Msa0226930

M. sativa cultivar zhongmu-4 / Msa0226930

KEGG: K08857
GO: GO:0000226 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0008152 GO:0008544 GO:0009888 GO:0009913 GO:0009987 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030154 GO:0030855 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048856 GO:0048869 GO:0055028 GO:0060429 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0226930	XP_003596869.3	91.912	952	69	2	1	945	52	1002	0.0	1718

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0226930	sp\|Q0WPH8\|NEK5_ARATH	48.485	957	406	28	1	940	56	942	0.0	711

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0226930	A0A396JGQ4	91.912	952	69	2	1	945	52	1002	0.0	1718

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0226930	Msa0259020	0.829882	4.013254e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0226930	MtrunA17_Chr2g0321841	91.912	952	69	2	1	945	52	1002	0.0	1718
Msa0226930	MtrunA17_Chr4g0025671	57.937	1008	323	16	1	944	56	1026	0.0	981
Msa0226930	MtrunA17_Chr1g0190831	68.778	221	69	0	1	221	52	272	5.62e-110	352
Msa0226930	MtrunA17_Chr1g0190831	65.079	63	22	0	873	935	546	608	6.20e-17	85.5
Msa0226930	MtrunA17_Chr7g0258051	67.532	231	69	1	1	231	64	288	1.26e-109	352
Msa0226930	MtrunA17_Chr7g0258051	78.000	50	11	0	884	933	583	632	4.53e-15	79.7
Msa0226930	MtrunA17_Chr7g0260191	66.222	225	76	0	1	225	52	276	5.51e-109	350
Msa0226930	MtrunA17_Chr7g0260191	68.966	58	18	0	877	934	553	610	1.93e-16	84.0
Msa0226930	MtrunA17_Chr1g0185221	58.175	263	108	2	1	261	52	314	9.71e-108	346
Msa0226930	MtrunA17_Chr1g0185221	64.615	65	23	0	874	938	537	601	1.19e-16	84.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0226930	AT3G44200.2	48.485	957	406	28	1	940	56	942	0.0	711
Msa0226930	AT3G44200.1	48.485	957	406	28	1	940	56	942	0.0	711
Msa0226930	AT3G20860.1	70.183	218	63	2	1	218	52	267	9.71e-110	345
Msa0226930	AT3G20860.1	45.370	108	50	3	835	933	295	402	3.19e-12	70.1
Msa0226930	AT1G54510.4	70.616	211	62	0	1	211	52	262	4.77e-108	347
Msa0226930	AT1G54510.4	72.414	58	16	0	877	934	532	589	6.69e-17	85.9
Msa0226930	AT1G54510.2	70.616	211	62	0	1	211	52	262	4.77e-108	347
Msa0226930	AT1G54510.2	72.414	58	16	0	877	934	532	589	6.69e-17	85.9
Msa0226930	AT1G54510.1	70.616	211	62	0	1	211	52	262	4.77e-108	347
Msa0226930	AT1G54510.1	72.414	58	16	0	877	934	532	589	6.69e-17	85.9

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Find 213 sgRNAs with CRISPR-Local

Find 486 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTAAGGTCTCCTCAA+GGG	0.624519	2_1:+77387491	Msa0226930:CDS
AAAAGTTGGTGTAAACGGAG+AGG	0.671095	2_1:-77386506	None:intergenic
AAACAGATAAACAGGAGGTT+AGG	0.444849	2_1:+77385569	Msa0226930:CDS
AAACATTCACTTTCCATTTC+TGG	0.153915	2_1:+77386724	Msa0226930:CDS
AAAGGCTCTCTAGAAACATG+TGG	0.658063	2_1:-77386265	None:intergenic
AAAGTAGAAACTTACAAGGA+TGG	0.543494	2_1:-77384738	None:intergenic
AAAGTTGGCACCTCTACATT+AGG	0.381919	2_1:-77385808	None:intergenic
AAATATTTGTAACAGGTTGT+AGG	0.401428	2_1:+77383340	Msa0226930:intron
AAATCAGATTTCGGTGTTAA+TGG	0.223289	2_1:+77385778	Msa0226930:CDS
AAATCTGATATTTGGTCCCT+AGG	0.465642	2_1:+77383415	Msa0226930:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCTTACATTGTTGAATTCA+AGG	+	chr2_1:77380806-77380825	Msa0226930:CDS	30.0%
TCCTTGAATTCAACAATGTA+AGG	-	chr2_1:77380810-77380829	None:intergenic	30.0%
TAAAAAGAACTAGTTGTTGG+AGG	-	chr2_1:77381114-77381133	None:intergenic	30.0%
AAACACAGAATATGAAACAG+AGG	+	chr2_1:77381288-77381307	Msa0226930:intron	30.0%
GTATCAATCAAACTGAAGTT+TGG	-	chr2_1:77381340-77381359	None:intergenic	30.0%
GTTTGATTGATACAACTGTT+TGG	+	chr2_1:77381347-77381366	Msa0226930:intron	30.0%
TAATCAAACTGAAGTTTGGA+TGG	-	chr2_1:77381454-77381473	None:intergenic	30.0%
CTCTTAATCAAACTGAAGTT+TGG	-	chr2_1:77381458-77381477	None:intergenic	30.0%
ATAAACAAGAATACCTCTTC+AGG	-	chr2_1:77381954-77381973	None:intergenic	30.0%
GCAAAACAAAATTTGAATGC+AGG	-	chr2_1:77382097-77382116	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	77380778	77387867	77380778	ID=Msa0226930;Name=Msa0226930
chr2_1	mRNA	77380778	77387867	77380778	ID=Msa0226930-mRNA-1;Parent=Msa0226930;Name=Msa0226930-mRNA-1;_AED=0.08;_eAED=0.08;_QI=2\|0.90\|0.91\|1\|1\|1\|12\|286\|945
chr2_1	exon	77380778	77380845	77380778	ID=Msa0226930-mRNA-1:exon:23512;Parent=Msa0226930-mRNA-1
chr2_1	exon	77381755	77381801	77381755	ID=Msa0226930-mRNA-1:exon:23513;Parent=Msa0226930-mRNA-1
chr2_1	exon	77381914	77381959	77381914	ID=Msa0226930-mRNA-1:exon:23514;Parent=Msa0226930-mRNA-1
chr2_1	exon	77382050	77382130	77382050	ID=Msa0226930-mRNA-1:exon:23515;Parent=Msa0226930-mRNA-1
chr2_1	exon	77382226	77382268	77382226	ID=Msa0226930-mRNA-1:exon:23516;Parent=Msa0226930-mRNA-1
chr2_1	exon	77382363	77382412	77382363	ID=Msa0226930-mRNA-1:exon:23517;Parent=Msa0226930-mRNA-1
chr2_1	exon	77383355	77383436	77383355	ID=Msa0226930-mRNA-1:exon:23518;Parent=Msa0226930-mRNA-1
chr2_1	exon	77383510	77383559	77383510	ID=Msa0226930-mRNA-1:exon:23519;Parent=Msa0226930-mRNA-1
chr2_1	exon	77384672	77384745	77384672	ID=Msa0226930-mRNA-1:exon:23520;Parent=Msa0226930-mRNA-1
chr2_1	exon	77385102	77385153	77385102	ID=Msa0226930-mRNA-1:exon:23521;Parent=Msa0226930-mRNA-1
chr2_1	exon	77385247	77385897	77385247	ID=Msa0226930-mRNA-1:exon:23522;Parent=Msa0226930-mRNA-1
chr2_1	exon	77385986	77387867	77385986	ID=Msa0226930-mRNA-1:exon:23523;Parent=Msa0226930-mRNA-1
chr2_1	five_prime_UTR	77380778	77380779	77380778	ID=Msa0226930-mRNA-1:five_prime_utr;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77380780	77380845	77380780	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77381755	77381801	77381755	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77381914	77381959	77381914	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77382050	77382130	77382050	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77382226	77382268	77382226	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77382363	77382412	77382363	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77383355	77383436	77383355	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77383510	77383559	77383510	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77384672	77384745	77384672	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77385102	77385153	77385102	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77385247	77385897	77385247	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	CDS	77385986	77387581	77385986	ID=Msa0226930-mRNA-1:cds;Parent=Msa0226930-mRNA-1
chr2_1	three_prime_UTR	77387582	77387867	77387582	ID=Msa0226930-mRNA-1:three_prime_utr;Parent=Msa0226930-mRNA-1

Gene Sequence

>Msa0226930

ATGGCTCTAATTGCGAGAATTAAGCATCCTTACATTGTTGAATTCAAGGAAGCTTGGGTTGAGAAGGGTTGCTATGTTTGCATTGTTACTGGATACTGTGAAGGTGGAGACATAGCAGCATTGATGAAAAAATCAAATGGGGCTTACTTTCCTGAAGAGAAACTCTGCAAATGGGTTACTCAATTACTGTTGGCAGTAGAATACCTGCATTCAAATTTTGTTTTGCACCGTGACCTAAAGTGTTCTAACATCTTTCTTACCAAGGATCATGATGTTCGCTTAGGGGATTTTGGGCTTGCTAAGACACTAAAAGCAGACGATTTAACTTCTTCGGTTGTAGGAACTCCCAACTATATGTGCCCTGAACTGCTTACAGATATTCCTTATGGATTCAAATCTGATATTTGGTCCCTAGGGTGTTGTATATATGAGATGGCTGCTCACCGCCCTGCTTTCAAAGCTTTTGATTTGGATGGATTGATAAGCAAGATCAACCGTTCCTCTATTGGTACTTTGCCTCCCTGTTATTCTCCATCCTTGAAAACACTGATAAAAGGCATGTTGAGGAAAAACCCCGAGCATCGTCCAACAGCATCCGATATCTTAAAGCACCCTTATTTGCAGCCTTATGTTCATCAATATTGTTCATCTCTCTGTTCTCCATCAGCCTCTTCTCCTGAAAAGCCAATTTTTGCTGTTCATGTTCCTCGAAAAACCATGGCAGAAAGTGAAAAAAGTAATAGTTTGTGTAGTGAAAAAGGTGGTTTGATGTCAAATAAGAGAAACGTTTCAAAAGCAGCACCAAAGGGTGATGACAAAATCACTGAGGTAGACCTGTCATCGATTGGTGACAAAATCACTGACCTTATCATGCCCTGTAGAGAAAGAAATAGCTCTAGCAATGTCAATGCCAAAACAGATAAACAGGAGGTTAGGAAGCAAACTCACCTTGAACACAAATCTAATGTTGGATCTAAGCAAGCAAGGATAGTTAAGAATGCAAAGATGGGACCAAAACATGAAAAGGTTAAAGAGATGAGTTCTCCAATGAGAGGGAGTCTGATGAAGGCTGGTGGTATATCAACACAGAAAATTAATACAGAGACCTTGTGTAAATTTCCGAAATCAGATTTCGGTGTTAATGGTTTGAAGCCTAATGTAGAGGTGCCAACTTTAGCACCTTCAAAAGCAACCCTTCATTCTGCAAAACGAATCCAGGGATCACATACTTCAAAACATCAGCAGTTACCGATGATAGAACCTACACCAAAAACTAAACCTCGACATAAGGTGACACCACCATGTGGTCCTATCAAACAAGTAGTAGCAGAACCTGAAGTTTCTGCACAGCCAAAACAAAAGACTCCTCCAAGCCTACTTAAGCCACCCTCATTTCTGGGACGCAATAAGCAAGCCGGGCATGATATGCCAAATGCAGCAAATGATAAAGAAAGGTTAGATACAAATAAAATAGCCCAGGAGGCTAAGATGAGACATCATCGGTTGCCTAACGGTCATCTGCCACATGTTTCTAGAGAGCCTTTGAAGGCTTTTGAAAGTAGTACCAAAGGAATGCAAACAACCAGCAACAATTCTGTTTCATCTTCAGTCTCTGTTCAAGGATTTGAACTTTTTGACTTTGCAAACACTTTTATTGACTTGAGTGAACCAACACCCCCTGAACATGGGAGTTTTAATCACACTGAAATTGTGGGATCACGTGCCAACAGTAGTGGACCTGATGAAAATATTTCTGGCGAATCCTCTCCGTTTACACCAACTTTTCAGAATACCATAACTCGTAATGAGAAAGTAAGTCCCAGGTTAACTCTGGATCACTCAGTGATGTATGATAGTTTTCTTATAAATCAAACAACTACATCTGATTCCGGTGAGTGCGATGTTCCGTCTGCCAAAGTCACACAAGAAATCAAAGACCTTCAAGATTTTTGTAAGGAAATGGCTTCAATCAATTCTCTCAAACATTCACTTTCCATTTCTGGAAAAAAGTCAGTTTGTGAAGTTAGTCCAGAAAGCTTGCCAAATAATAGGCCTCATAAAGTTAGCCTGCCAAAGCTTATGTGCATCTCAACTGGTGATGACAAATTCATGGTGAGGGAAAGAGTGTCCTCAGTGGCTGAAATTGCTCCTGTGAATATATCTACTAAAATTTCAAGGCAGAAAGTTTTACAAGAAGAGAAGGGAATGGTTTTACAGAATCTAGCAACAGAAAGACCAGCTTCTGGCCATCTTCCCCGAGCTCTCGATGATGTCATCCATGTTATACGCCATAGCAGTTACCGGGTAGGGAGTGATCTTCCAGTAATGGAATCAACTGAGAAGGGAGTTACAAACGTAGATGTTGGTACACTCATCAACGTTGTAAAGGATGATTTAGAAATGAGAAATGTGGGAACTCCTTTGTCCCTTAAATCTTCAAATTGCTCTGAAGTTAATAGCTTGAACTCAAATACTATTGATAATTTGGAAATCAGAAACTTGAGCTTCAAGTCAAGTATTTCTGAACATCCTGGCGTTGAAGAGCAAGATGCGAGAAACCCTGATTTCGCTAAATATAACAAATATAGCCTTCCTACAGCTGAAGAGGAAATGCCAGAAAAGGAATCATTGGATGTTAAATCTTCCAAACAGAGAGCGGATGCACTTGAAGGGCTGTTAGAATTGTCTGCAGATTTGCTGCAGCAAAATAGATTGGAGGAACTTGCAGTTGTTCTCAAGCCCTTTGGAAAAGGTAAGGTCTCCTCAAGGGAGACAGCTATTTGGTTGGCCAAGAGTCTAAAAGGATTGATGGTTGAGGAAAGTAGGGGACGTAGTTAG

Protein sequence

>Msa0226930

MALIARIKHPYIVEFKEAWVEKGCYVCIVTGYCEGGDIAALMKKSNGAYFPEEKLCKWVTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDHDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLTDIPYGFKSDIWSLGCCIYEMAAHRPAFKAFDLDGLISKINRSSIGTLPPCYSPSLKTLIKGMLRKNPEHRPTASDILKHPYLQPYVHQYCSSLCSPSASSPEKPIFAVHVPRKTMAESEKSNSLCSEKGGLMSNKRNVSKAAPKGDDKITEVDLSSIGDKITDLIMPCRERNSSSNVNAKTDKQEVRKQTHLEHKSNVGSKQARIVKNAKMGPKHEKVKEMSSPMRGSLMKAGGISTQKINTETLCKFPKSDFGVNGLKPNVEVPTLAPSKATLHSAKRIQGSHTSKHQQLPMIEPTPKTKPRHKVTPPCGPIKQVVAEPEVSAQPKQKTPPSLLKPPSFLGRNKQAGHDMPNAANDKERLDTNKIAQEAKMRHHRLPNGHLPHVSREPLKAFESSTKGMQTTSNNSVSSSVSVQGFELFDFANTFIDLSEPTPPEHGSFNHTEIVGSRANSSGPDENISGESSPFTPTFQNTITRNEKVSPRLTLDHSVMYDSFLINQTTTSDSGECDVPSAKVTQEIKDLQDFCKEMASINSLKHSLSISGKKSVCEVSPESLPNNRPHKVSLPKLMCISTGDDKFMVRERVSSVAEIAPVNISTKISRQKVLQEEKGMVLQNLATERPASGHLPRALDDVIHVIRHSSYRVGSDLPVMESTEKGVTNVDVGTLINVVKDDLEMRNVGTPLSLKSSNCSEVNSLNSNTIDNLEIRNLSFKSSISEHPGVEEQDARNPDFAKYNKYSLPTAEEEMPEKESLDVKSSKQRADALEGLLELSADLLQQNRLEELAVVLKPFGKGKVSSRETAIWLAKSLKGLMVEESRGRS*