AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0228820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228820 MtrunA17_Chr2g0281071 71.373 255 34 10 1 245 120 345 1.76e-73 227
Msa0228820 MtrunA17_Chr4g0048511 38.007 271 110 14 1 245 120 358 7.84e-22 92.8
Msa0228820 MtrunA17_Chr2g0323411 52.564 78 25 5 2 69 120 195 4.52e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0228820 AT4G21440.1 60.563 71 23 1 1 66 119 189 1.16e-16 78.6

Find 86 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAACTTGGTCTTGAAAT+TGG 0.182407 2_2:-155472 None:intergenic
GCAAATATACCTGCATTCTT+TGG 0.201925 2_2:-154709 None:intergenic
AGCTATAATAATAATTATTA+TGG 0.235606 2_2:+155704 Msa0228820:CDS
GTTGCATGCCAAGGAGATTT+TGG 0.243513 2_2:-155301 None:intergenic
ATTCAGTTACATAGCATTCT+TGG 0.244592 2_2:+154944 Msa0228820:five_prime_UTR
GATTTAACTGTTGATGATTA+TGG 0.249291 2_2:-155954 None:intergenic
TAGTCCTAGACTTGACTTAT+TGG 0.262917 2_2:+155214 Msa0228820:CDS
TATGTTTGATAAGCTAAATT+AGG 0.300671 2_2:-154548 None:intergenic
TCAAACAAATGTGATTGATT+TGG 0.313222 2_2:-155560 None:intergenic
ATTCTATGTTCATCTCTCTA+TGG 0.320999 2_2:+155248 Msa0228820:CDS
ATTTCATTGTCCGTCCTTCC+TGG 0.322882 2_2:-155125 None:intergenic
CCTTGGCATGCAACAACCTT+TGG 0.333556 2_2:+155310 Msa0228820:CDS
TATAATCTTGTTGATGTTGT+TGG 0.333703 2_2:-155385 None:intergenic
AAAGCTATGAAAGTTGTAAT+AGG 0.344023 2_2:+155855 Msa0228820:CDS
AGAAACATGGTTATGGCAAT+TGG 0.355623 2_2:+154677 Msa0228820:five_prime_UTR
CTAAGGTCGGTGAAATTTGA+TGG 0.370501 2_2:-155602 None:intergenic
TCAAACTCCACGTGTATCAT+TGG 0.377726 2_2:+155809 Msa0228820:CDS
CAAATATACCTGCATTCTTT+GGG 0.380676 2_2:-154708 None:intergenic
GGTGAATTTAAACTCTTGTA+AGG 0.389328 2_2:-154527 None:intergenic
TACATTCAGAAACATGGTTA+TGG 0.393040 2_2:+154670 Msa0228820:five_prime_UTR
CATTAGGTACTTGTTGAACT+TGG 0.404953 2_2:-155484 None:intergenic
GATGGATATGTATTCACATT+GGG 0.405959 2_2:-155584 None:intergenic
TGTGTGTGCTTGGATTTAAT+TGG 0.410308 2_2:+155929 Msa0228820:three_prime_UTR
TGGAAGTTGTTCATTATTTG+AGG 0.412921 2_2:-155281 None:intergenic
GGAACTTGGTTCTGTATTTG+AGG 0.414212 2_2:-155434 None:intergenic
ATTACTATCAAAATATTGAT+TGG 0.414298 2_2:+155627 Msa0228820:CDS
GTACAAGCATTATCAGCATT+AGG 0.421505 2_2:-155500 None:intergenic
ACTATTGGAACACACACATT+AGG 0.422347 2_2:+155153 Msa0228820:five_prime_UTR
ATTGATTGGTACGAAAATGA+TGG 0.426971 2_2:+155641 Msa0228820:CDS
GGGTGAAATAATCAACTGTT+AGG 0.438487 2_2:-155979 None:intergenic
ATATATAGCTATGAATCCTT+TGG 0.440517 2_2:+156017 Msa0228820:three_prime_UTR
CCTTGTTGTGAGAAGAATAA+TGG 0.449193 2_2:+154598 Msa0228820:five_prime_UTR
TATTATAGCTTGATAGTTGA+GGG 0.452326 2_2:-155691 None:intergenic
GCAAAAGTTGTCGTCTCCGC+TGG 0.453551 2_2:+154858 Msa0228820:five_prime_UTR
TGATCCTCCTCAGTTGTCCA+TGG 0.458297 2_2:-154634 None:intergenic
TTCGCTTCTAAAATATGTGC+AGG 0.460016 2_2:-155893 None:intergenic
AAAGTAGAGAAAGAAAATAG+AGG 0.460884 2_2:-154471 None:intergenic
AAGAATAATGGTCTCAAGAA+AGG 0.479998 2_2:+154610 Msa0228820:five_prime_UTR
TTGATAGTAATTACTAAGGT+CGG 0.480724 2_2:-155615 None:intergenic
TTCTGTATTTGAGGATTACA+AGG 0.484329 2_2:-155425 None:intergenic
GGAACACACACATTAGGAAA+AGG 0.485344 2_2:+155159 Msa0228820:five_prime_UTR
GAAGCTAACTTTAGAAGCTC+AGG 0.487104 2_2:-155338 None:intergenic
AACAACTTCCAAAATCTCCT+TGG 0.494886 2_2:+155293 Msa0228820:CDS
AGGGACATAATCATCTGTTA+AGG 0.502211 2_2:-155672 None:intergenic
TGATGGATATGTATTCACAT+TGG 0.503073 2_2:-155585 None:intergenic
GAGGAGGATCAGAAACTCAT+TGG 0.513810 2_2:+154646 Msa0228820:five_prime_UTR
CAAACTCCACGTGTATCATT+GGG 0.513925 2_2:+155810 Msa0228820:CDS
CCAAAGGTTGTTGCATGCCA+AGG 0.520611 2_2:-155310 None:intergenic
AGTCTAGGACTATGTGTCAC+TGG 0.521633 2_2:-155203 None:intergenic
AAGTAGAGAAAGAAAATAGA+GGG 0.522270 2_2:-154470 None:intergenic
AGGTCCAATAAGTCAAGTCT+AGG 0.528293 2_2:-155218 None:intergenic
ATAGTAAGCACAATTTGCTA+TGG 0.531269 2_2:+154568 Msa0228820:five_prime_UTR
TATAGCTATGAATCCTTTGG+TGG 0.535937 2_2:+156020 Msa0228820:three_prime_UTR
TTATTATAGCTTGATAGTTG+AGG 0.539302 2_2:-155692 None:intergenic
GAGTTTGAATTCAACGGTGT+GGG 0.541396 2_2:-155794 None:intergenic
AAGGTTGAGCTAAATAGTAA+TGG 0.546481 2_2:-154508 None:intergenic
CAAAATGTGAAAGTGGAACT+TGG 0.548815 2_2:-155448 None:intergenic
TGAATAGGTTTGCAAAGATG+TGG 0.566583 2_2:+154836 Msa0228820:intron
ACAATTGCATCTCGATTACC+AGG 0.573205 2_2:+155107 Msa0228820:five_prime_UTR
ATAGCTATGAATCCTTTGGT+GGG 0.574251 2_2:+156021 Msa0228820:three_prime_UTR
AATTGGACAAAATGTGAAAG+TGG 0.575573 2_2:-155455 None:intergenic
CCATTATTCTTCTCACAACA+AGG 0.578512 2_2:-154598 None:intergenic
TTGATGTTGTTGGTGTGATG+AGG 0.580363 2_2:-155375 None:intergenic
AACATGGTTATGGCAATTGG+AGG 0.580738 2_2:+154680 Msa0228820:five_prime_UTR
AGAAGCTCAGGATTCACCAA+AGG 0.600325 2_2:-155326 None:intergenic
CACGTGGAGTTTGAATTCAA+CGG 0.603350 2_2:-155800 None:intergenic
GGAGTTTGAATTCAACGGTG+TGG 0.610134 2_2:-155795 None:intergenic
AGGACACTCCCAAAGAATGC+AGG 0.611901 2_2:+154700 Msa0228820:five_prime_UTR
ATTGGTTACATTCAGAAACA+TGG 0.616475 2_2:+154664 Msa0228820:five_prime_UTR
ATCTCGATTACCAGGAAGGA+CGG 0.618625 2_2:+155115 Msa0228820:five_prime_UTR
TCGCTTCTAAAATATGTGCA+GGG 0.623098 2_2:-155892 None:intergenic
TTGCATCTCGATTACCAGGA+AGG 0.634311 2_2:+155111 Msa0228820:five_prime_UTR
ATATCAACATGCCCCACCAA+AGG 0.639972 2_2:-156033 None:intergenic
TGATGAGGTACTGAAGAGTG+AGG 0.641633 2_2:-155360 None:intergenic
AACACAAGGTGTAGTGAATG+TGG 0.648265 2_2:-155525 None:intergenic
ATGAACATAGAATAGAAGAG+AGG 0.652399 2_2:-155238 None:intergenic
AGGACCATGGACAACTGAGG+AGG 0.657016 2_2:+154630 Msa0228820:five_prime_UTR
GTCGGAGATAGTTTGTCCAG+CGG 0.665235 2_2:-154874 None:intergenic
ATGGTCTCAAGAAAGGACCA+TGG 0.674045 2_2:+154617 Msa0228820:five_prime_UTR
CTCCGACCAGATATAAAACG+TGG 0.676493 2_2:+154890 Msa0228820:five_prime_UTR
TAGCTATGAATCCTTTGGTG+GGG 0.676703 2_2:+156022 Msa0228820:three_prime_UTR
GAAAGGACCATGGACAACTG+AGG 0.679174 2_2:+154627 Msa0228820:five_prime_UTR
GACCAGATATAAAACGTGGT+CGG 0.697361 2_2:+154894 Msa0228820:five_prime_UTR
GGCAATGAAGAAGAAACACA+AGG 0.698458 2_2:-155539 None:intergenic
GTTGCTCCCAATGATACACG+TGG 0.742288 2_2:-155816 None:intergenic
GGTGTGGGACTAGAAGATGA+TGG 0.748637 2_2:-155779 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTGATATTAACTAAT+TGG + chr2_2:154792-154811 Msa0228820:intron 10.0%
!!! TTATTAATTTTCTATTGAAT+AGG + chr2_2:154821-154840 Msa0228820:intron 10.0%
!! AGCTATAATAATAATTATTA+TGG + chr2_2:155704-155723 Msa0228820:CDS 10.0%
!! TTTATTTGATATTAACTAAT+TGG + chr2_2:154792-154811 Msa0228820:intron 10.0%
!!! TTATTAATTTTCTATTGAAT+AGG + chr2_2:154821-154840 Msa0228820:intron 10.0%
!! AGCTATAATAATAATTATTA+TGG + chr2_2:155704-155723 Msa0228820:CDS 10.0%
!! ACAATGAAATAAAAAACTAT+TGG + chr2_2:155138-155157 Msa0228820:five_prime_UTR 15.0%
!!! TATTTTGATAGTAATTACTA+AGG - chr2_2:155622-155641 None:intergenic 15.0%
!! ATTACTATCAAAATATTGAT+TGG + chr2_2:155627-155646 Msa0228820:CDS 15.0%
!! ACAATGAAATAAAAAACTAT+TGG + chr2_2:155138-155157 Msa0228820:five_prime_UTR 15.0%
!!! TATTTTGATAGTAATTACTA+AGG - chr2_2:155622-155641 None:intergenic 15.0%
!! ATTACTATCAAAATATTGAT+TGG + chr2_2:155627-155646 Msa0228820:CDS 15.0%
!! TGAAAAAGAAAATGAATGAA+TGG - chr2_2:154450-154469 None:intergenic 20.0%
!! TATGTTTGATAAGCTAAATT+AGG - chr2_2:154551-154570 None:intergenic 20.0%
!! TGAAAAAGAAAATGAATGAA+TGG - chr2_2:154450-154469 None:intergenic 20.0%
!! TATGTTTGATAAGCTAAATT+AGG - chr2_2:154551-154570 None:intergenic 20.0%
! AAGTAGAGAAAGAAAATAGA+GGG - chr2_2:154473-154492 None:intergenic 25.0%
! AAAGTAGAGAAAGAAAATAG+AGG - chr2_2:154474-154493 None:intergenic 25.0%
! TCTATACACATAGCAAAAAA+AGG - chr2_2:154989-155008 None:intergenic 25.0%
! TATAATCTTGTTGATGTTGT+TGG - chr2_2:155388-155407 None:intergenic 25.0%
! TCAAACAAATGTGATTGATT+TGG - chr2_2:155563-155582 None:intergenic 25.0%
!! TTGATAGTAATTACTAAGGT+CGG - chr2_2:155618-155637 None:intergenic 25.0%
!! TATTATAGCTTGATAGTTGA+GGG - chr2_2:155694-155713 None:intergenic 25.0%
!! TTATTATAGCTTGATAGTTG+AGG - chr2_2:155695-155714 None:intergenic 25.0%
! AAAGCTATGAAAGTTGTAAT+AGG + chr2_2:155855-155874 Msa0228820:CDS 25.0%
!!! GATTTAACTGTTGATGATTA+TGG - chr2_2:155957-155976 None:intergenic 25.0%
! ATATATAGCTATGAATCCTT+TGG + chr2_2:156017-156036 Msa0228820:three_prime_UTR 25.0%
! AAGTAGAGAAAGAAAATAGA+GGG - chr2_2:154473-154492 None:intergenic 25.0%
! AAAGTAGAGAAAGAAAATAG+AGG - chr2_2:154474-154493 None:intergenic 25.0%
! TCTATACACATAGCAAAAAA+AGG - chr2_2:154989-155008 None:intergenic 25.0%
! TATAATCTTGTTGATGTTGT+TGG - chr2_2:155388-155407 None:intergenic 25.0%
! TCAAACAAATGTGATTGATT+TGG - chr2_2:155563-155582 None:intergenic 25.0%
!! TTGATAGTAATTACTAAGGT+CGG - chr2_2:155618-155637 None:intergenic 25.0%
!! TATTATAGCTTGATAGTTGA+GGG - chr2_2:155694-155713 None:intergenic 25.0%
!! TTATTATAGCTTGATAGTTG+AGG - chr2_2:155695-155714 None:intergenic 25.0%
! AAAGCTATGAAAGTTGTAAT+AGG + chr2_2:155855-155874 Msa0228820:CDS 25.0%
!!! GATTTAACTGTTGATGATTA+TGG - chr2_2:155957-155976 None:intergenic 25.0%
! ATATATAGCTATGAATCCTT+TGG + chr2_2:156017-156036 Msa0228820:three_prime_UTR 25.0%
AAGGTTGAGCTAAATAGTAA+TGG - chr2_2:154511-154530 None:intergenic 30.0%
GGTGAATTTAAACTCTTGTA+AGG - chr2_2:154530-154549 None:intergenic 30.0%
!! ATAGTAAGCACAATTTGCTA+TGG + chr2_2:154568-154587 Msa0228820:five_prime_UTR 30.0%
AAGAATAATGGTCTCAAGAA+AGG + chr2_2:154610-154629 Msa0228820:five_prime_UTR 30.0%
ATTGGTTACATTCAGAAACA+TGG + chr2_2:154664-154683 Msa0228820:five_prime_UTR 30.0%
TACATTCAGAAACATGGTTA+TGG + chr2_2:154670-154689 Msa0228820:five_prime_UTR 30.0%
CAAATATACCTGCATTCTTT+GGG - chr2_2:154711-154730 None:intergenic 30.0%
ATTCAGTTACATAGCATTCT+TGG + chr2_2:154944-154963 Msa0228820:five_prime_UTR 30.0%
CATAGCAAAAAAAGGAATAG+AGG - chr2_2:154981-155000 None:intergenic 30.0%
ATGAACATAGAATAGAAGAG+AGG - chr2_2:155241-155260 None:intergenic 30.0%
ATTCTATGTTCATCTCTCTA+TGG + chr2_2:155248-155267 Msa0228820:CDS 30.0%
TGGAAGTTGTTCATTATTTG+AGG - chr2_2:155284-155303 None:intergenic 30.0%
TTCTGTATTTGAGGATTACA+AGG - chr2_2:155428-155447 None:intergenic 30.0%
AATTGGACAAAATGTGAAAG+TGG - chr2_2:155458-155477 None:intergenic 30.0%
GATGGATATGTATTCACATT+GGG - chr2_2:155587-155606 None:intergenic 30.0%
! TGATGGATATGTATTCACAT+TGG - chr2_2:155588-155607 None:intergenic 30.0%
ATTGATTGGTACGAAAATGA+TGG + chr2_2:155641-155660 Msa0228820:CDS 30.0%
! TTTTATGTAATGTGTGTGCT+TGG + chr2_2:155919-155938 Msa0228820:exon 30.0%
TATATCCCAGCAAAAACATA+GGG - chr2_2:156002-156021 None:intergenic 30.0%
ATATATCCCAGCAAAAACAT+AGG - chr2_2:156003-156022 None:intergenic 30.0%
AAGGTTGAGCTAAATAGTAA+TGG - chr2_2:154511-154530 None:intergenic 30.0%
GGTGAATTTAAACTCTTGTA+AGG - chr2_2:154530-154549 None:intergenic 30.0%
!! ATAGTAAGCACAATTTGCTA+TGG + chr2_2:154568-154587 Msa0228820:five_prime_UTR 30.0%
AAGAATAATGGTCTCAAGAA+AGG + chr2_2:154610-154629 Msa0228820:five_prime_UTR 30.0%
ATTGGTTACATTCAGAAACA+TGG + chr2_2:154664-154683 Msa0228820:five_prime_UTR 30.0%
TACATTCAGAAACATGGTTA+TGG + chr2_2:154670-154689 Msa0228820:five_prime_UTR 30.0%
CAAATATACCTGCATTCTTT+GGG - chr2_2:154711-154730 None:intergenic 30.0%
ATTCAGTTACATAGCATTCT+TGG + chr2_2:154944-154963 Msa0228820:five_prime_UTR 30.0%
CATAGCAAAAAAAGGAATAG+AGG - chr2_2:154981-155000 None:intergenic 30.0%
ATGAACATAGAATAGAAGAG+AGG - chr2_2:155241-155260 None:intergenic 30.0%
ATTCTATGTTCATCTCTCTA+TGG + chr2_2:155248-155267 Msa0228820:CDS 30.0%
TGGAAGTTGTTCATTATTTG+AGG - chr2_2:155284-155303 None:intergenic 30.0%
TTCTGTATTTGAGGATTACA+AGG - chr2_2:155428-155447 None:intergenic 30.0%
AATTGGACAAAATGTGAAAG+TGG - chr2_2:155458-155477 None:intergenic 30.0%
GATGGATATGTATTCACATT+GGG - chr2_2:155587-155606 None:intergenic 30.0%
! TGATGGATATGTATTCACAT+TGG - chr2_2:155588-155607 None:intergenic 30.0%
ATTGATTGGTACGAAAATGA+TGG + chr2_2:155641-155660 Msa0228820:CDS 30.0%
! TTTTATGTAATGTGTGTGCT+TGG + chr2_2:155919-155938 Msa0228820:exon 30.0%
TATATCCCAGCAAAAACATA+GGG - chr2_2:156002-156021 None:intergenic 30.0%
ATATATCCCAGCAAAAACAT+AGG - chr2_2:156003-156022 None:intergenic 30.0%
CCATTATTCTTCTCACAACA+AGG - chr2_2:154601-154620 None:intergenic 35.0%
! CCTTGTTGTGAGAAGAATAA+TGG + chr2_2:154598-154617 Msa0228820:five_prime_UTR 35.0%
AGAAACATGGTTATGGCAAT+TGG + chr2_2:154677-154696 Msa0228820:five_prime_UTR 35.0%
GCAAATATACCTGCATTCTT+TGG - chr2_2:154712-154731 None:intergenic 35.0%
TGAATAGGTTTGCAAAGATG+TGG + chr2_2:154836-154855 Msa0228820:intron 35.0%
GTCGACAAAGTTAGTATGAA+CGG - chr2_2:155066-155085 None:intergenic 35.0%
TTCATACTAACTTTGTCGAC+CGG + chr2_2:155066-155085 Msa0228820:intron 35.0%
ACTATTGGAACACACACATT+AGG + chr2_2:155153-155172 Msa0228820:five_prime_UTR 35.0%
!! TAGGAAAAGGCTTTTGAGAA+TGG + chr2_2:155172-155191 Msa0228820:exon 35.0%
!! AGGAAAAGGCTTTTGAGAAT+GGG + chr2_2:155173-155192 Msa0228820:exon 35.0%
TAGTCCTAGACTTGACTTAT+TGG + chr2_2:155214-155233 Msa0228820:CDS 35.0%
AACAACTTCCAAAATCTCCT+TGG + chr2_2:155293-155312 Msa0228820:CDS 35.0%
CAAAATGTGAAAGTGGAACT+TGG - chr2_2:155451-155470 None:intergenic 35.0%
!! GTTGAACTTGGTCTTGAAAT+TGG - chr2_2:155475-155494 None:intergenic 35.0%
! CATTAGGTACTTGTTGAACT+TGG - chr2_2:155487-155506 None:intergenic 35.0%
GTACAAGCATTATCAGCATT+AGG - chr2_2:155503-155522 None:intergenic 35.0%
AGGGACATAATCATCTGTTA+AGG - chr2_2:155675-155694 None:intergenic 35.0%
!! TCGCTTCTAAAATATGTGCA+GGG - chr2_2:155895-155914 None:intergenic 35.0%
!! TTCGCTTCTAAAATATGTGC+AGG - chr2_2:155896-155915 None:intergenic 35.0%
TGTGTGTGCTTGGATTTAAT+TGG + chr2_2:155929-155948 Msa0228820:three_prime_UTR 35.0%
GGGTGAAATAATCAACTGTT+AGG - chr2_2:155982-156001 None:intergenic 35.0%
!! ATTTCACCCTATGTTTTTGC+TGG + chr2_2:155993-156012 Msa0228820:three_prime_UTR 35.0%
!! TTTCACCCTATGTTTTTGCT+GGG + chr2_2:155994-156013 Msa0228820:three_prime_UTR 35.0%
TATAGCTATGAATCCTTTGG+TGG + chr2_2:156020-156039 Msa0228820:three_prime_UTR 35.0%
ATAGCTATGAATCCTTTGGT+GGG + chr2_2:156021-156040 Msa0228820:three_prime_UTR 35.0%
CCATTATTCTTCTCACAACA+AGG - chr2_2:154601-154620 None:intergenic 35.0%
! CCTTGTTGTGAGAAGAATAA+TGG + chr2_2:154598-154617 Msa0228820:five_prime_UTR 35.0%
AGAAACATGGTTATGGCAAT+TGG + chr2_2:154677-154696 Msa0228820:five_prime_UTR 35.0%
GCAAATATACCTGCATTCTT+TGG - chr2_2:154712-154731 None:intergenic 35.0%
TGAATAGGTTTGCAAAGATG+TGG + chr2_2:154836-154855 Msa0228820:intron 35.0%
GTCGACAAAGTTAGTATGAA+CGG - chr2_2:155066-155085 None:intergenic 35.0%
TTCATACTAACTTTGTCGAC+CGG + chr2_2:155066-155085 Msa0228820:intron 35.0%
ACTATTGGAACACACACATT+AGG + chr2_2:155153-155172 Msa0228820:five_prime_UTR 35.0%
!! TAGGAAAAGGCTTTTGAGAA+TGG + chr2_2:155172-155191 Msa0228820:exon 35.0%
!! AGGAAAAGGCTTTTGAGAAT+GGG + chr2_2:155173-155192 Msa0228820:exon 35.0%
TAGTCCTAGACTTGACTTAT+TGG + chr2_2:155214-155233 Msa0228820:CDS 35.0%
AACAACTTCCAAAATCTCCT+TGG + chr2_2:155293-155312 Msa0228820:CDS 35.0%
CAAAATGTGAAAGTGGAACT+TGG - chr2_2:155451-155470 None:intergenic 35.0%
!! GTTGAACTTGGTCTTGAAAT+TGG - chr2_2:155475-155494 None:intergenic 35.0%
! CATTAGGTACTTGTTGAACT+TGG - chr2_2:155487-155506 None:intergenic 35.0%
GTACAAGCATTATCAGCATT+AGG - chr2_2:155503-155522 None:intergenic 35.0%
AGGGACATAATCATCTGTTA+AGG - chr2_2:155675-155694 None:intergenic 35.0%
!! TCGCTTCTAAAATATGTGCA+GGG - chr2_2:155895-155914 None:intergenic 35.0%
!! TTCGCTTCTAAAATATGTGC+AGG - chr2_2:155896-155915 None:intergenic 35.0%
TGTGTGTGCTTGGATTTAAT+TGG + chr2_2:155929-155948 Msa0228820:three_prime_UTR 35.0%
GGGTGAAATAATCAACTGTT+AGG - chr2_2:155982-156001 None:intergenic 35.0%
!! ATTTCACCCTATGTTTTTGC+TGG + chr2_2:155993-156012 Msa0228820:three_prime_UTR 35.0%
!! TTTCACCCTATGTTTTTGCT+GGG + chr2_2:155994-156013 Msa0228820:three_prime_UTR 35.0%
TATAGCTATGAATCCTTTGG+TGG + chr2_2:156020-156039 Msa0228820:three_prime_UTR 35.0%
ATAGCTATGAATCCTTTGGT+GGG + chr2_2:156021-156040 Msa0228820:three_prime_UTR 35.0%
AACATGGTTATGGCAATTGG+AGG + chr2_2:154680-154699 Msa0228820:five_prime_UTR 40.0%
! GACCACGTTTTATATCTGGT+CGG - chr2_2:154895-154914 None:intergenic 40.0%
GACCAGATATAAAACGTGGT+CGG + chr2_2:154894-154913 Msa0228820:five_prime_UTR 40.0%
! AACCGACCACGTTTTATATC+TGG - chr2_2:154899-154918 None:intergenic 40.0%
!! TGGTCGGTTTTCATTTGAAG+AGG + chr2_2:154910-154929 Msa0228820:five_prime_UTR 40.0%
TAGACCACCTATAATAGAGC+CGG - chr2_2:155088-155107 None:intergenic 40.0%
ACAATTGCATCTCGATTACC+AGG + chr2_2:155107-155126 Msa0228820:five_prime_UTR 40.0%
GGAACACACACATTAGGAAA+AGG + chr2_2:155159-155178 Msa0228820:five_prime_UTR 40.0%
AGGTCCAATAAGTCAAGTCT+AGG - chr2_2:155221-155240 None:intergenic 40.0%
! GAAGCTAACTTTAGAAGCTC+AGG - chr2_2:155341-155360 None:intergenic 40.0%
TTGATGTTGTTGGTGTGATG+AGG - chr2_2:155378-155397 None:intergenic 40.0%
!! GGAACTTGGTTCTGTATTTG+AGG - chr2_2:155437-155456 None:intergenic 40.0%
AACACAAGGTGTAGTGAATG+TGG - chr2_2:155528-155547 None:intergenic 40.0%
GGCAATGAAGAAGAAACACA+AGG - chr2_2:155542-155561 None:intergenic 40.0%
CTAAGGTCGGTGAAATTTGA+TGG - chr2_2:155605-155624 None:intergenic 40.0%
GAGTTTGAATTCAACGGTGT+GGG - chr2_2:155797-155816 None:intergenic 40.0%
CACGTGGAGTTTGAATTCAA+CGG - chr2_2:155803-155822 None:intergenic 40.0%
TCAAACTCCACGTGTATCAT+TGG + chr2_2:155809-155828 Msa0228820:CDS 40.0%
CAAACTCCACGTGTATCATT+GGG + chr2_2:155810-155829 Msa0228820:CDS 40.0%
TAGCTATGAATCCTTTGGTG+GGG + chr2_2:156022-156041 Msa0228820:three_prime_UTR 40.0%
AACATGGTTATGGCAATTGG+AGG + chr2_2:154680-154699 Msa0228820:five_prime_UTR 40.0%
! GACCACGTTTTATATCTGGT+CGG - chr2_2:154895-154914 None:intergenic 40.0%
GACCAGATATAAAACGTGGT+CGG + chr2_2:154894-154913 Msa0228820:five_prime_UTR 40.0%
! AACCGACCACGTTTTATATC+TGG - chr2_2:154899-154918 None:intergenic 40.0%
!! TGGTCGGTTTTCATTTGAAG+AGG + chr2_2:154910-154929 Msa0228820:five_prime_UTR 40.0%
TAGACCACCTATAATAGAGC+CGG - chr2_2:155088-155107 None:intergenic 40.0%
ACAATTGCATCTCGATTACC+AGG + chr2_2:155107-155126 Msa0228820:five_prime_UTR 40.0%
GGAACACACACATTAGGAAA+AGG + chr2_2:155159-155178 Msa0228820:five_prime_UTR 40.0%
AGGTCCAATAAGTCAAGTCT+AGG - chr2_2:155221-155240 None:intergenic 40.0%
! GAAGCTAACTTTAGAAGCTC+AGG - chr2_2:155341-155360 None:intergenic 40.0%
TTGATGTTGTTGGTGTGATG+AGG - chr2_2:155378-155397 None:intergenic 40.0%
!! GGAACTTGGTTCTGTATTTG+AGG - chr2_2:155437-155456 None:intergenic 40.0%
AACACAAGGTGTAGTGAATG+TGG - chr2_2:155528-155547 None:intergenic 40.0%
GGCAATGAAGAAGAAACACA+AGG - chr2_2:155542-155561 None:intergenic 40.0%
CTAAGGTCGGTGAAATTTGA+TGG - chr2_2:155605-155624 None:intergenic 40.0%
GAGTTTGAATTCAACGGTGT+GGG - chr2_2:155797-155816 None:intergenic 40.0%
CACGTGGAGTTTGAATTCAA+CGG - chr2_2:155803-155822 None:intergenic 40.0%
TCAAACTCCACGTGTATCAT+TGG + chr2_2:155809-155828 Msa0228820:CDS 40.0%
CAAACTCCACGTGTATCATT+GGG + chr2_2:155810-155829 Msa0228820:CDS 40.0%
TAGCTATGAATCCTTTGGTG+GGG + chr2_2:156022-156041 Msa0228820:three_prime_UTR 40.0%
ATGGTCTCAAGAAAGGACCA+TGG + chr2_2:154617-154636 Msa0228820:five_prime_UTR 45.0%
GAGGAGGATCAGAAACTCAT+TGG + chr2_2:154646-154665 Msa0228820:five_prime_UTR 45.0%
CTCCGACCAGATATAAAACG+TGG + chr2_2:154890-154909 Msa0228820:five_prime_UTR 45.0%
! TTGTCGACCGGCTCTATTAT+AGG + chr2_2:155078-155097 Msa0228820:intron 45.0%
TTGCATCTCGATTACCAGGA+AGG + chr2_2:155111-155130 Msa0228820:five_prime_UTR 45.0%
ATCTCGATTACCAGGAAGGA+CGG + chr2_2:155115-155134 Msa0228820:five_prime_UTR 45.0%
ATTTCATTGTCCGTCCTTCC+TGG - chr2_2:155128-155147 None:intergenic 45.0%
! AGTCTAGGACTATGTGTCAC+TGG - chr2_2:155206-155225 None:intergenic 45.0%
! GTTGCATGCCAAGGAGATTT+TGG - chr2_2:155304-155323 None:intergenic 45.0%
AGAAGCTCAGGATTCACCAA+AGG - chr2_2:155329-155348 None:intergenic 45.0%
!! TGATGAGGTACTGAAGAGTG+AGG - chr2_2:155363-155382 None:intergenic 45.0%
GGAGTTTGAATTCAACGGTG+TGG - chr2_2:155798-155817 None:intergenic 45.0%
ATATCAACATGCCCCACCAA+AGG - chr2_2:156036-156055 None:intergenic 45.0%
ATGGTCTCAAGAAAGGACCA+TGG + chr2_2:154617-154636 Msa0228820:five_prime_UTR 45.0%
GAGGAGGATCAGAAACTCAT+TGG + chr2_2:154646-154665 Msa0228820:five_prime_UTR 45.0%
CTCCGACCAGATATAAAACG+TGG + chr2_2:154890-154909 Msa0228820:five_prime_UTR 45.0%
! TTGTCGACCGGCTCTATTAT+AGG + chr2_2:155078-155097 Msa0228820:intron 45.0%
TTGCATCTCGATTACCAGGA+AGG + chr2_2:155111-155130 Msa0228820:five_prime_UTR 45.0%
ATCTCGATTACCAGGAAGGA+CGG + chr2_2:155115-155134 Msa0228820:five_prime_UTR 45.0%
ATTTCATTGTCCGTCCTTCC+TGG - chr2_2:155128-155147 None:intergenic 45.0%
! AGTCTAGGACTATGTGTCAC+TGG - chr2_2:155206-155225 None:intergenic 45.0%
! GTTGCATGCCAAGGAGATTT+TGG - chr2_2:155304-155323 None:intergenic 45.0%
AGAAGCTCAGGATTCACCAA+AGG - chr2_2:155329-155348 None:intergenic 45.0%
!! TGATGAGGTACTGAAGAGTG+AGG - chr2_2:155363-155382 None:intergenic 45.0%
GGAGTTTGAATTCAACGGTG+TGG - chr2_2:155798-155817 None:intergenic 45.0%
ATATCAACATGCCCCACCAA+AGG - chr2_2:156036-156055 None:intergenic 45.0%
GAAAGGACCATGGACAACTG+AGG + chr2_2:154627-154646 Msa0228820:five_prime_UTR 50.0%
TGATCCTCCTCAGTTGTCCA+TGG - chr2_2:154637-154656 None:intergenic 50.0%
AGGACACTCCCAAAGAATGC+AGG + chr2_2:154700-154719 Msa0228820:five_prime_UTR 50.0%
! GTCGGAGATAGTTTGTCCAG+CGG - chr2_2:154877-154896 None:intergenic 50.0%
! TCGACCGGCTCTATTATAGG+TGG + chr2_2:155081-155100 Msa0228820:intron 50.0%
CCAAAGGTTGTTGCATGCCA+AGG - chr2_2:155313-155332 None:intergenic 50.0%
CCTTGGCATGCAACAACCTT+TGG + chr2_2:155310-155329 Msa0228820:CDS 50.0%
GGTGTGGGACTAGAAGATGA+TGG - chr2_2:155782-155801 None:intergenic 50.0%
GTTGCTCCCAATGATACACG+TGG - chr2_2:155819-155838 None:intergenic 50.0%
GAAAGGACCATGGACAACTG+AGG + chr2_2:154627-154646 Msa0228820:five_prime_UTR 50.0%
TGATCCTCCTCAGTTGTCCA+TGG - chr2_2:154637-154656 None:intergenic 50.0%
AGGACACTCCCAAAGAATGC+AGG + chr2_2:154700-154719 Msa0228820:five_prime_UTR 50.0%
! GTCGGAGATAGTTTGTCCAG+CGG - chr2_2:154877-154896 None:intergenic 50.0%
! TCGACCGGCTCTATTATAGG+TGG + chr2_2:155081-155100 Msa0228820:intron 50.0%
CCAAAGGTTGTTGCATGCCA+AGG - chr2_2:155313-155332 None:intergenic 50.0%
CCTTGGCATGCAACAACCTT+TGG + chr2_2:155310-155329 Msa0228820:CDS 50.0%
GGTGTGGGACTAGAAGATGA+TGG - chr2_2:155782-155801 None:intergenic 50.0%
GTTGCTCCCAATGATACACG+TGG - chr2_2:155819-155838 None:intergenic 50.0%
AGGACCATGGACAACTGAGG+AGG + chr2_2:154630-154649 Msa0228820:five_prime_UTR 55.0%
GCAAAAGTTGTCGTCTCCGC+TGG + chr2_2:154858-154877 Msa0228820:five_prime_UTR 55.0%
AGGACCATGGACAACTGAGG+AGG + chr2_2:154630-154649 Msa0228820:five_prime_UTR 55.0%
GCAAAAGTTGTCGTCTCCGC+TGG + chr2_2:154858-154877 Msa0228820:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 154440 156060 154440 ID=Msa0228820;Name=Msa0228820
chr2_2 mRNA 154440 156060 154440 ID=Msa0228820-mRNA-1;Parent=Msa0228820;Name=Msa0228820-mRNA-1;_AED=0.05;_eAED=0.05;_QI=503|1|1|1|1|1|3|132|245
chr2_2 exon 154440 154721 154440 ID=Msa0228820-mRNA-1:exon:46;Parent=Msa0228820-mRNA-1
chr2_2 exon 154843 154972 154843 ID=Msa0228820-mRNA-1:exon:47;Parent=Msa0228820-mRNA-1
chr2_2 exon 155100 156060 155100 ID=Msa0228820-mRNA-1:exon:48;Parent=Msa0228820-mRNA-1
chr2_2 five_prime_UTR 154440 154721 154440 ID=Msa0228820-mRNA-1:five_prime_utr;Parent=Msa0228820-mRNA-1
chr2_2 five_prime_UTR 154843 154972 154843 ID=Msa0228820-mRNA-1:five_prime_utr;Parent=Msa0228820-mRNA-1
chr2_2 five_prime_UTR 155100 155190 155100 ID=Msa0228820-mRNA-1:five_prime_utr;Parent=Msa0228820-mRNA-1
chr2_2 CDS 155191 155928 155191 ID=Msa0228820-mRNA-1:cds;Parent=Msa0228820-mRNA-1
chr2_2 three_prime_UTR 155929 156060 155929 ID=Msa0228820-mRNA-1:three_prime_utr;Parent=Msa0228820-mRNA-1
Gene Sequence

>Msa0228820

ATGGGAATTGATCCAGTGACACATAGTCCTAGACTTGACTTATTGGACCTCTCTTCTATTCTATGTTCATCTCTCTATGGTAATTCATCACCTCAAATAATGAACAACTTCCAAAATCTCCTTGGCATGCAACAACCTTTGGTGAATCCTGAGCTTCTAAAGTTAGCTTCCTCACTCTTCAGTACCTCATCACACCAACAACATCAACAAGATTATAATAATAATAATAATCAACCTTGTAATCCTCAAATACAGAACCAAGTTCCACTTTCACATTTTGTCCAATTTCAAGACCAAGTTCAACAAGTACCTAATGCTGATAATGCTTGTACAACCACATTCACTACACCTTGTGTTTCTTCTTCATTGCCAAATCAATCACATTTGTTTGAACCCAATGTGAATACATATCCATCAAATTTCACCGACCTTAGTAATTACTATCAAAATATTGATTGGTACGAAAATGATGGATTTTCTACCTTAACAGATGATTATGTCCCTCAACTATCAAGCTATAATAATAATTATTATGGTTCTGATAATCAAAATCTTATGTATCAAACTTCAAATCAACTTCTATCAACACCATCATCTTCTAGTCCCACACCGTTGAATTCAAACTCCACGTGTATCATTGGGAGCAACACAGAAGATGAGAGAGAAAGCTATGAAAGTTGTAATAGGTTAAATTTTGAAATCCCTGCACATATTTTAGAAGCGAATGATTTTATGTAA
Protein sequence

>Msa0228820

MGIDPVTHSPRLDLLDLSSILCSSLYGNSSPQIMNNFQNLLGMQQPLVNPELLKLASSLFSTSSHQQHQQDYNNNNNQPCNPQIQNQVPLSHFVQFQDQVQQVPNADNACTTTFTTPCVSSSLPNQSHLFEPNVNTYPSNFTDLSNYYQNIDWYENDGFSTLTDDYVPQLSSYNNNYYGSDNQNLMYQTSNQLLSTPSSSSPTPLNSNSTCIIGSNTEDERESYESCNRLNFEIPAHILEANDFM*