AlfalfaGEDB | Msa1425850

M. sativa cultivar zhongmu-4 / Msa1425850

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0009611 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1425850	QSD99628.1	86.450	369	36	10	1	364	1	360	0.0	581

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1425850	sp\|Q9LDR8\|MY102_ARATH	52.547	373	144	11	1	364	1	349	1.13e-106	320

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1425850	A0A896WEJ8	86.450	369	36	10	1	364	1	360	0.0	581

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TFs/TRs:

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Gene ID	Type	Classification
Msa1425850	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0228820	Msa1425850	0.924605	5.631956e-90	-8.615850e-47
Msa0311880	Msa1425850	0.903475	3.339958e-79	-8.615850e-47
Msa0829600	Msa1425850	0.967946	5.535542e-128	-8.615850e-47
Msa1425850	Msa1451640	0.829957	3.849489e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1425850	MtrunA17_Chr2g0281071	79.947	374	36	10	1	364	1	345	1.21e-171	481
Msa1425850	MtrunA17_Chr4g0048511	55.641	390	115	14	1	364	1	358	1.99e-118	347
Msa1425850	MtrunA17_Chr2g0323411	73.232	198	40	6	1	188	1	195	2.38e-94	284
Msa1425850	MtrunA17_Chr7g0217741	75.373	134	32	1	1	134	1	133	8.98e-76	236
Msa1425850	MtrunA17_Chr3g0115821	74.627	134	33	1	1	134	1	133	6.62e-75	235
Msa1425850	MtrunA17_Chr6g0453471	57.071	198	80	2	1	198	1	193	8.11e-75	235
Msa1425850	MtrunA17_Chr5g0410961	57.843	204	73	4	1	201	1	194	3.80e-74	233
Msa1425850	MtrunA17_Chr4g0042671	76.515	132	30	1	1	132	1	131	5.34e-74	235
Msa1425850	MtrunA17_Chr4g0039971	69.388	147	44	1	1	147	2	147	8.06e-74	233
Msa1425850	MtrunA17_Chr5g0395281	48.235	255	107	7	1	243	1	242	1.57e-68	218

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1425850	AT4G21440.1	52.547	373	144	11	1	364	1	349	1.15e-107	320
Msa1425850	AT4G05100.1	64.341	258	66	5	1	232	1	258	1.10e-99	298
Msa1425850	AT4G28110.1	86.923	130	16	1	1	130	1	129	2.75e-83	255
Msa1425850	AT5G54230.1	64.500	200	63	5	13	208	12	207	2.01e-79	246
Msa1425850	AT5G16770.4	63.536	181	65	1	1	181	1	180	7.50e-79	245
Msa1425850	AT5G16770.3	63.536	181	65	1	1	181	1	180	7.50e-79	245
Msa1425850	AT5G16770.1	63.536	181	65	1	1	181	1	180	2.68e-78	244
Msa1425850	AT5G16770.2	63.536	181	65	1	1	181	1	180	2.68e-78	244
Msa1425850	AT3G02940.2	77.206	136	30	1	1	136	1	135	1.85e-76	238
Msa1425850	AT3G02940.1	77.206	136	30	1	1	136	1	135	4.58e-76	238

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Find 87 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTATGAAAGTTGTAAT+AGG	0.344023	tig0025900:-24043	Msa1425850:CDS
AAAGTAGAGAAAGAAAATAG+AGG	0.460884	tig0025900:+25428	Msa1425850:intergenic
AACAACTTCCAAAATCTCCT+TGG	0.494886	tig0025900:-24605	Msa1425850:CDS
AACACAAGGTGTAGTGAATG+TGG	0.648265	tig0025900:+24373	Msa1425850:intergenic
AACATGGTTATGGCAATTGG+AGG	0.580738	tig0025900:-25218	Msa1425850:CDS
AAGAATAATGGTCTCAAGAA+AGG	0.479998	tig0025900:-25288	Msa1425850:CDS
AAGGTTGAGCTAAATAGTAA+TGG	0.546481	tig0025900:+25391	Msa1425850:intergenic
AAGTAGAGAAAGAAAATAGA+GGG	0.522270	tig0025900:+25429	Msa1425850:intergenic
AATTGGACAAAATGTGAAAG+TGG	0.575573	tig0025900:+24443	Msa1425850:intergenic
ACAATTGCATCTCGATTACC+AGG	0.573205	tig0025900:-24791	Msa1425850:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGTTGAGCTAAATAGTAA+TGG	+	tig0025900:23931-23950	Msa1028890:intergenic	30.0%
GGTGAATTTAAACTCTTGTA+AGG	+	tig0025900:23950-23969	Msa1028890:intergenic	30.0%
AAGAATAATGGTCTCAAGAA+AGG	-	tig0025900:24031-24050	Msa1425850:CDS	30.0%
ATTGGTTACATTCAGAAACA+TGG	-	tig0025900:24085-24104	Msa1425850:CDS	30.0%
TACATTCAGAAACATGGTTA+TGG	-	tig0025900:24091-24110	Msa1425850:CDS	30.0%
CAAATATACCTGCATTCTTT+GGG	+	tig0025900:24132-24151	Msa1425850:intergenic	30.0%
ATTCAGTTACATAGCATTCT+TGG	-	tig0025900:24365-24384	Msa1425850:CDS	30.0%
CATAGCAAAAAAAGGAATAG+AGG	+	tig0025900:24402-24421	Msa1425850:intergenic	30.0%
ATGAACATAGAATAGAAGAG+AGG	+	tig0025900:24662-24681	Msa1425850:intergenic	30.0%
ATTCTATGTTCATCTCTCTA+TGG	-	tig0025900:24669-24688	Msa1425850:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0025900	gene	23860	25481	23860	ID=Msa1425850;Name=Msa1425850
tig0025900	mRNA	23860	25481	23860	ID=Msa1425850-mRNA-1;Parent=Msa1425850;Name=Msa1425850-mRNA-1;_AED=0.05;_eAED=0.05;_QI=147\|1\|1\|1\|1\|1\|3\|132\|364
tig0025900	exon	23860	24820	23860	ID=Msa1425850-mRNA-1:exon:4501;Parent=Msa1425850-mRNA-1
tig0025900	exon	24948	25077	24948	ID=Msa1425850-mRNA-1:exon:4500;Parent=Msa1425850-mRNA-1
tig0025900	exon	25199	25481	25199	ID=Msa1425850-mRNA-1:exon:4499;Parent=Msa1425850-mRNA-1
tig0025900	five_prime_UTR	25335	25481	25335	ID=Msa1425850-mRNA-1:five_prime_utr;Parent=Msa1425850-mRNA-1
tig0025900	CDS	25199	25334	25199	ID=Msa1425850-mRNA-1:cds;Parent=Msa1425850-mRNA-1
tig0025900	CDS	24948	25077	24948	ID=Msa1425850-mRNA-1:cds;Parent=Msa1425850-mRNA-1
tig0025900	CDS	23992	24820	23992	ID=Msa1425850-mRNA-1:cds;Parent=Msa1425850-mRNA-1
tig0025900	three_prime_UTR	23860	23991	23860	ID=Msa1425850-mRNA-1:three_prime_utr;Parent=Msa1425850-mRNA-1

Gene Sequence

>Msa1425850

ATGGGAAGAACACCTTGTTGTGAGAAGAATAATGGTCTCAAGAAAGGACCATGGACAACTGAGGAGGATCAGAAACTCATTGGTTACATTCAGAAACATGGTTATGGCAATTGGAGGACACTCCCAAAGAATGCAGGTTTGCAAAGATGTGGCAAAAGTTGTCGTCTCCGCTGGACAAACTATCTCCGACCAGATATAAAACGTGGTCGGTTTTCATTTGAAGAGGAAGAGACAATTATTCAGTTACATAGCATTCTTGGCAACAAGTGGTCTACAATTGCATCTCGATTACCAGGAAGGACGGACAATGAAATAAAAAACTATTGGAACACACACATTAGGAAAAGGCTTTTGAGAATGGGAATTGATCCAGTGACACATAGTCCTAGACTTGACTTATTGGACCTCTCTTCTATTCTATGTTCATCTCTCTATGGTAATTCATCACCTCAAATAATGAACAACTTCCAAAATCTCCTTGGCATGCAACAACCTTTGGTGAATCCTGAGCTTCTAAAGTTAGCTTCCTCACTCTTCAGTACCTCATCACACCAACAACATCAACAAGATTATAATAATAATAATAATCAACCTTGTAATCCTCAAATACAGAACCAAGTTCCACTTTCACATTTTGTCCAATTTCAAGACCAAGTTCAACAAGTACCTAATGCTGATAATGCTTGTACAACCACATTCACTACACCTTGTGTTTCTTCTTCATTGCCAAATCAATCACATTTGTTTGAACCCAATGTGAATACATATCCATCAAATTTCACCGACCTTAGTAATTACTATCAAAATATTGATTGGTACGAAAATGATGGATTTTCTACCTTAACAGATGATTATGTCCCTCAACTATCAAGCTATAATAATAATTATTATGGTTCTGATAATCAAAATCTTATGTATCAAACTTCAAATCAACTTCTATCAACACCATCATCTTCTAGTCCCACACCGTTGAATTCAAACTCCACGTGTATCATTGGGAGCAACACAGAAGATGAGAGAGAAAGCTATGAAAGTTGTAATAGGTTAAATTTTGAAATCCCTGCACATATTTTAGAAGCGAATGATTTTATGTAA

Protein sequence

>Msa1425850

MGRTPCCEKNNGLKKGPWTTEEDQKLIGYIQKHGYGNWRTLPKNAGLQRCGKSCRLRWTNYLRPDIKRGRFSFEEEETIIQLHSILGNKWSTIASRLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHSPRLDLLDLSSILCSSLYGNSSPQIMNNFQNLLGMQQPLVNPELLKLASSLFSTSSHQQHQQDYNNNNNQPCNPQIQNQVPLSHFVQFQDQVQQVPNADNACTTTFTTPCVSSSLPNQSHLFEPNVNTYPSNFTDLSNYYQNIDWYENDGFSTLTDDYVPQLSSYNNNYYGSDNQNLMYQTSNQLLSTPSSSSPTPLNSNSTCIIGSNTEDERESYESCNRLNFEIPAHILEANDFM*