AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0229600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0229600 MtrunA17_Chr1g0160711 67.586 290 67 7 4 270 2 287 7.37e-126 372
Msa0229600 MtrunA17_Chr1g0160761 63.087 298 78 6 1 270 1 294 8.46e-113 338
Msa0229600 MtrunA17_Chr1g0160661 51.186 295 109 7 1 270 27 311 1.49e-82 256
Msa0229600 MtrunA17_Chr1g0160701 51.264 277 106 7 18 269 33 305 1.19e-72 234
Msa0229600 MtrunA17_Chr1g0160561 46.711 304 121 7 3 270 7 305 2.04e-71 231
Msa0229600 MtrunA17_Chr1g0160721 46.602 309 116 8 1 270 12 310 7.36e-70 227
Msa0229600 MtrunA17_Chr1g0158061 35.714 210 102 6 33 214 35 239 6.46e-27 105
Msa0229600 MtrunA17_Chr3g0088331 31.600 250 137 9 22 249 35 272 5.34e-26 103
Msa0229600 MtrunA17_Chr1g0158151 35.135 222 106 8 25 213 27 243 4.94e-25 104
Msa0229600 MtrunA17_Chr1g0157791 35.398 226 98 9 24 214 28 240 2.82e-24 102
Msa0229600 MtrunA17_Chr6g0467711 35.047 214 99 8 30 214 45 247 3.50e-24 101
Msa0229600 MtrunA17_Chr1g0158411 30.290 241 134 8 2 213 15 250 1.54e-23 99.8
Msa0229600 MtrunA17_Chr1g0157961 36.681 229 97 14 24 216 33 249 4.33e-23 95.9
Msa0229600 MtrunA17_Chr1g0157991 30.928 291 142 13 24 266 28 307 1.13e-22 97.4
Msa0229600 MtrunA17_Chr1g0157881 30.175 285 106 12 33 242 60 326 2.14e-22 96.7
Msa0229600 MtrunA17_Chr1g0158761 33.906 233 120 11 24 232 24 246 4.05e-22 95.5
Msa0229600 MtrunA17_Chr1g0158191 35.808 229 100 12 24 213 24 244 1.00e-21 94.4
Msa0229600 MtrunA17_Chr1g0158661 30.645 248 133 10 15 233 25 262 6.14e-21 92.0
Msa0229600 MtrunA17_Chr1g0158631 31.356 236 113 9 15 212 25 249 3.26e-20 90.1
Msa0229600 MtrunA17_Chr1g0158461 28.788 264 133 9 1 214 16 274 1.52e-19 86.3
Msa0229600 MtrunA17_Chr1g0158041 33.333 216 104 7 33 214 36 245 2.18e-19 87.8
Msa0229600 MtrunA17_Chr1g0158541 30.544 239 131 10 5 213 16 249 5.69e-19 86.3
Msa0229600 MtrunA17_Chr1g0158331 28.626 262 131 10 1 215 16 268 1.60e-18 84.0
Msa0229600 MtrunA17_Chr1g0158731 28.163 245 139 7 1 213 1 240 6.20e-18 81.6
Msa0229600 MtrunA17_Chr1g0157911 27.306 271 145 10 8 242 13 267 1.18e-17 82.4
Msa0229600 MtrunA17_Chr1g0157861 42.500 120 56 4 12 128 30 139 4.86e-17 80.9
Msa0229600 MtrunA17_Chr1g0158121 45.263 95 45 3 24 116 29 118 4.08e-15 70.5
Msa0229600 MtrunA17_Chr1g0158301 27.530 247 139 10 1 213 7 247 1.95e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACGTTTATCGACTAAAAT+AGG 0.246719 2_2:+1368727 Msa0229600:CDS
TAATTTCACCATACGACTAT+TGG 0.252802 2_2:+1368073 Msa0229600:CDS
ATCCCTCCATATTATTCTTT+AGG 0.258183 2_2:+1368125 Msa0229600:CDS
TCTTTGTAAGAGTACCTTAT+AGG 0.258868 2_2:-1369201 None:intergenic
GATTGTTGTCTTCACATAAT+AGG 0.261172 2_2:-1367983 None:intergenic
TTTATGGATTTGAACTTTCT+TGG 0.267862 2_2:+1368469 Msa0229600:CDS
TCTTGTAGCAGTCAAATTAT+TGG 0.278750 2_2:+1369311 Msa0229600:three_prime_UTR
CTCTTGTCACCACATTCTTC+TGG 0.284196 2_2:-1367954 None:intergenic
AACCAGCCTCATTGTAAATA+TGG 0.286693 2_2:+1368540 Msa0229600:CDS
ATAACATCAGCCACCCTTTC+AGG 0.289456 2_2:+1367919 Msa0229600:CDS
GGTAAATCTCTATCGGAATT+CGG 0.309259 2_2:+1368345 Msa0229600:CDS
TTCCTTAACGTCCTTTGTTA+TGG 0.311343 2_2:+1367818 Msa0229600:CDS
CTTTGTAAGAGTACCTTATA+GGG 0.320325 2_2:-1369200 None:intergenic
ACTCAAGGAATAATTCTCTT+TGG 0.327172 2_2:-1369505 None:intergenic
TAGATTCCTAAAGAATAATA+TGG 0.337829 2_2:-1368131 None:intergenic
AGCGGTGTTAAATTTGTTCT+TGG 0.345849 2_2:+1369076 Msa0229600:CDS
AGTTTATGTATCTCACTTCT+TGG 0.350053 2_2:+1368391 Msa0229600:CDS
TCGGAATTCGGGCTGGGAGA+TGG 0.350153 2_2:+1368357 Msa0229600:CDS
TTTGAACTTTCTTGGCTCAA+TGG 0.379007 2_2:+1368477 Msa0229600:CDS
GTAAATCTCTATCGGAATTC+GGG 0.390404 2_2:+1368346 Msa0229600:CDS
ACAATAGCATCATCCCTATA+AGG 0.400813 2_2:+1369187 Msa0229600:three_prime_UTR
ATGTTCTTGCAGATTACTAC+TGG 0.411034 2_2:+1368683 Msa0229600:intron
ATCTCTATCGGAATTCGGGC+TGG 0.412568 2_2:+1368350 Msa0229600:CDS
ATATATGAATTTATGCCAAA+TGG 0.415581 2_2:+1369453 Msa0229600:three_prime_UTR
GTTTATGTATCTCACTTCTT+GGG 0.422997 2_2:+1368392 Msa0229600:CDS
CTAATTAGCAACAAAGTGTA+TGG 0.423091 2_2:+1367855 Msa0229600:CDS
CGGAATTCGGGCTGGGAGAT+GGG 0.430299 2_2:+1368358 Msa0229600:CDS
TCGAGATAATAGAGTAGGTA+GGG 0.434332 2_2:-1368182 None:intergenic
CACCATAACAAAGGACGTTA+AGG 0.446569 2_2:-1367820 None:intergenic
TGGAGGGATGGAATTATTAG+AGG 0.448658 2_2:-1368111 None:intergenic
TCGAAGTTGTCCTTTGAAAA+CGG 0.456219 2_2:-1369280 None:intergenic
ATTGACAAAATCTTGATCAC+TGG 0.457843 2_2:-1369346 None:intergenic
GTTAATGTGGTGCAGCTCAT+TGG 0.477445 2_2:+1369399 Msa0229600:three_prime_UTR
AACATAGGTTTCGTCAATAT+AGG 0.477692 2_2:-1368221 None:intergenic
GTCAATGAAGTTGCTAGCAT+TGG 0.479006 2_2:+1369363 Msa0229600:three_prime_UTR
AAACTAGGCAATGGAGGGTA+TGG 0.487547 2_2:+1369255 Msa0229600:three_prime_UTR
TCTCTATCGGAATTCGGGCT+GGG 0.494794 2_2:+1368351 Msa0229600:CDS
CTCAATGGTCTTTGTAAACA+TGG 0.495007 2_2:+1368492 Msa0229600:CDS
CGAGATAATAGAGTAGGTAG+GGG 0.501077 2_2:-1368181 None:intergenic
GATTAAAATACAATGATCAA+TGG 0.510348 2_2:-1369103 None:intergenic
AAAATCTTGATCACTGGACT+TGG 0.516719 2_2:-1369340 None:intergenic
AACCATATTTACAATGAGGC+TGG 0.517738 2_2:-1368542 None:intergenic
CGCATGGAATATTCATACAT+TGG 0.525641 2_2:+1368261 Msa0229600:CDS
TTGAACAGCTCACATAACAT+AGG 0.526678 2_2:-1368236 None:intergenic
CTTAACGTCCTTTGTTATGG+TGG 0.529770 2_2:+1367821 Msa0229600:CDS
GAACAATTCAAGACAAAACT+AGG 0.546680 2_2:+1369240 Msa0229600:three_prime_UTR
GTCGAGATAATAGAGTAGGT+AGG 0.547359 2_2:-1368183 None:intergenic
TAGCTATGGTAAATCTCTAT+CGG 0.548828 2_2:+1368338 Msa0229600:intron
ATACAGTTGTTCGCAACTCA+AGG 0.554329 2_2:-1369520 None:intergenic
TTCCTAAAGAATAATATGGA+GGG 0.556324 2_2:-1368127 None:intergenic
AAACATCTATCAATGTATGA+TGG 0.560371 2_2:+1369141 Msa0229600:three_prime_UTR
TAATTAGCAACAAAGTGTAT+GGG 0.560784 2_2:+1367856 Msa0229600:CDS
ACTACTGGGATAATGTGGCT+TGG 0.564962 2_2:+1368698 Msa0229600:CDS
AAGACAAAACTAGGCAATGG+AGG 0.565832 2_2:+1369249 Msa0229600:three_prime_UTR
ATTGAAATCCAATAGTCGTA+TGG 0.568248 2_2:-1368081 None:intergenic
ATGAAGTTGCTAGCATTGGT+AGG 0.571277 2_2:+1369367 Msa0229600:three_prime_UTR
TGTGAGCTGTTCAAATCGCA+TGG 0.571910 2_2:+1368245 Msa0229600:CDS
TAAAGAATAATATGGAGGGA+TGG 0.575078 2_2:-1368123 None:intergenic
ATCCATAAAACATCATATGA+CGG 0.575198 2_2:-1368455 None:intergenic
TTCAAGACAAAACTAGGCAA+TGG 0.579669 2_2:+1369246 Msa0229600:three_prime_UTR
CTTTGTCGAGATAATAGAGT+AGG 0.589840 2_2:-1368187 None:intergenic
ATGGTCTTTGTAAACATGGT+CGG 0.597059 2_2:+1368496 Msa0229600:CDS
ATTCCTAAAGAATAATATGG+AGG 0.605055 2_2:-1368128 None:intergenic
TGTTCTTGCAGATTACTACT+GGG 0.610146 2_2:+1368684 Msa0229600:intron
AGATTACTACTGGGATAATG+TGG 0.611780 2_2:+1368693 Msa0229600:intron
GAGTCATGTTCAAGAAAGTG+TGG 0.617950 2_2:+1367891 Msa0229600:CDS
CAGAAGAATGTGGTGACAAG+AGG 0.621774 2_2:+1367955 Msa0229600:CDS
AGACAAAACTAGGCAATGGA+GGG 0.623349 2_2:+1369250 Msa0229600:three_prime_UTR
AGGTTTCGTCAATATAGGAT+CGG 0.624414 2_2:-1368216 None:intergenic
AATTAGCAACAAAGTGTATG+GGG 0.626387 2_2:+1367857 Msa0229600:CDS
TTCAAAGGACAACTTCGAAG+TGG 0.631987 2_2:+1369285 Msa0229600:three_prime_UTR
TATGAATTTGAAGAATACCA+TGG 0.632125 2_2:+1368020 Msa0229600:CDS
GAGATAATAGAGTAGGTAGG+GGG 0.640864 2_2:-1368180 None:intergenic
TACCAACCATATTTACAATG+AGG 0.643138 2_2:-1368546 None:intergenic
CACTGCTGCCACCATAACAA+AGG 0.651819 2_2:-1367829 None:intergenic
TAGGATTCACCATGTTAATG+TGG 0.664872 2_2:+1369386 Msa0229600:three_prime_UTR
TGAGCTGCACCACATTAACA+TGG 0.688780 2_2:-1369395 None:intergenic
AAAGACAGTCCAGAAGAATG+TGG 0.689791 2_2:+1367945 Msa0229600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGTATAAATAGTAATAT+TGG + chr2_2:1368873-1368892 Msa0229600:intron 10.0%
!! ATAGTATAAATAGTAATATT+GGG + chr2_2:1368874-1368893 Msa0229600:intron 10.0%
!!! TTTTTAAAAACAACAAAATA+CGG - chr2_2:1368940-1368959 None:intergenic 10.0%
!! AATAGTATAAATAGTAATAT+TGG + chr2_2:1368873-1368892 Msa0229600:intron 10.0%
!! ATAGTATAAATAGTAATATT+GGG + chr2_2:1368874-1368893 Msa0229600:intron 10.0%
!!! TTTTTAAAAACAACAAAATA+CGG - chr2_2:1368940-1368959 None:intergenic 10.0%
!!! ATATACTCTTTTTTTCAATT+TGG + chr2_2:1368568-1368587 Msa0229600:intron 15.0%
!!! TTTTAAAAACAACAAAATAC+GGG - chr2_2:1368939-1368958 None:intergenic 15.0%
!!! ATTGTATTTTAATCTACAAA+TGG + chr2_2:1369112-1369131 Msa0229600:exon 15.0%
!!! ATATACTCTTTTTTTCAATT+TGG + chr2_2:1368568-1368587 Msa0229600:intron 15.0%
!!! TTTTAAAAACAACAAAATAC+GGG - chr2_2:1368939-1368958 None:intergenic 15.0%
!!! ATTGTATTTTAATCTACAAA+TGG + chr2_2:1369112-1369131 Msa0229600:exon 15.0%
!! TAGATTCCTAAAGAATAATA+TGG - chr2_2:1368134-1368153 None:intergenic 20.0%
!!! CATAAATAACATTTTTTGCT+CGG + chr2_2:1368428-1368447 Msa0229600:CDS 20.0%
!!! AACTTTTAGTACAAGAATTA+AGG + chr2_2:1368789-1368808 Msa0229600:intron 20.0%
!!! ACTTTTAGTACAAGAATTAA+GGG + chr2_2:1368790-1368809 Msa0229600:intron 20.0%
!!! TATTTTTAGCATTAGTTCTT+TGG + chr2_2:1368900-1368919 Msa0229600:intron 20.0%
!!! TTTGTTGTTTTTAAAAAACG+TGG + chr2_2:1368943-1368962 Msa0229600:intron 20.0%
!! GATTAAAATACAATGATCAA+TGG - chr2_2:1369106-1369125 None:intergenic 20.0%
!! ATATATGAATTTATGCCAAA+TGG + chr2_2:1369453-1369472 Msa0229600:three_prime_UTR 20.0%
!! TAGATTCCTAAAGAATAATA+TGG - chr2_2:1368134-1368153 None:intergenic 20.0%
!!! CATAAATAACATTTTTTGCT+CGG + chr2_2:1368428-1368447 Msa0229600:CDS 20.0%
!!! AACTTTTAGTACAAGAATTA+AGG + chr2_2:1368789-1368808 Msa0229600:intron 20.0%
!!! ACTTTTAGTACAAGAATTAA+GGG + chr2_2:1368790-1368809 Msa0229600:intron 20.0%
!!! TATTTTTAGCATTAGTTCTT+TGG + chr2_2:1368900-1368919 Msa0229600:intron 20.0%
!!! TTTGTTGTTTTTAAAAAACG+TGG + chr2_2:1368943-1368962 Msa0229600:intron 20.0%
!! GATTAAAATACAATGATCAA+TGG - chr2_2:1369106-1369125 None:intergenic 20.0%
!! ATATATGAATTTATGCCAAA+TGG + chr2_2:1369453-1369472 Msa0229600:three_prime_UTR 20.0%
! TAATTAGCAACAAAGTGTAT+GGG + chr2_2:1367856-1367875 Msa0229600:CDS 25.0%
! TATGAATTTGAAGAATACCA+TGG + chr2_2:1368020-1368039 Msa0229600:CDS 25.0%
! TTCCTAAAGAATAATATGGA+GGG - chr2_2:1368130-1368149 None:intergenic 25.0%
! ATTCCTAAAGAATAATATGG+AGG - chr2_2:1368131-1368150 None:intergenic 25.0%
! ATCCATAAAACATCATATGA+CGG - chr2_2:1368458-1368477 None:intergenic 25.0%
! TTTATGGATTTGAACTTTCT+TGG + chr2_2:1368469-1368488 Msa0229600:CDS 25.0%
!!! TTTTTCAATTTTCACTTTGC+AGG + chr2_2:1369034-1369053 Msa0229600:intron 25.0%
! AAACATCTATCAATGTATGA+TGG + chr2_2:1369141-1369160 Msa0229600:three_prime_UTR 25.0%
!!! TAGTTTTGTCTTGAATTGTT+CGG - chr2_2:1369241-1369260 None:intergenic 25.0%
! TAATTAGCAACAAAGTGTAT+GGG + chr2_2:1367856-1367875 Msa0229600:CDS 25.0%
! TATGAATTTGAAGAATACCA+TGG + chr2_2:1368020-1368039 Msa0229600:CDS 25.0%
! TTCCTAAAGAATAATATGGA+GGG - chr2_2:1368130-1368149 None:intergenic 25.0%
! ATTCCTAAAGAATAATATGG+AGG - chr2_2:1368131-1368150 None:intergenic 25.0%
! ATCCATAAAACATCATATGA+CGG - chr2_2:1368458-1368477 None:intergenic 25.0%
! TTTATGGATTTGAACTTTCT+TGG + chr2_2:1368469-1368488 Msa0229600:CDS 25.0%
!!! TTTTTCAATTTTCACTTTGC+AGG + chr2_2:1369034-1369053 Msa0229600:intron 25.0%
! AAACATCTATCAATGTATGA+TGG + chr2_2:1369141-1369160 Msa0229600:three_prime_UTR 25.0%
!!! TAGTTTTGTCTTGAATTGTT+CGG - chr2_2:1369241-1369260 None:intergenic 25.0%
CTAATTAGCAACAAAGTGTA+TGG + chr2_2:1367855-1367874 Msa0229600:CDS 30.0%
AATTAGCAACAAAGTGTATG+GGG + chr2_2:1367857-1367876 Msa0229600:CDS 30.0%
GATTGTTGTCTTCACATAAT+AGG - chr2_2:1367986-1368005 None:intergenic 30.0%
! ATTGCACATAGTATTTTCCA+TGG - chr2_2:1368040-1368059 None:intergenic 30.0%
TAATTTCACCATACGACTAT+TGG + chr2_2:1368073-1368092 Msa0229600:CDS 30.0%
ATTGAAATCCAATAGTCGTA+TGG - chr2_2:1368084-1368103 None:intergenic 30.0%
TAAAGAATAATATGGAGGGA+TGG - chr2_2:1368126-1368145 None:intergenic 30.0%
ATCCCTCCATATTATTCTTT+AGG + chr2_2:1368125-1368144 Msa0229600:CDS 30.0%
AACATAGGTTTCGTCAATAT+AGG - chr2_2:1368224-1368243 None:intergenic 30.0%
TCTTTCTATGTTACTAGCTA+TGG + chr2_2:1368324-1368343 Msa0229600:intron 30.0%
TAGCTATGGTAAATCTCTAT+CGG + chr2_2:1368338-1368357 Msa0229600:intron 30.0%
AGTTTATGTATCTCACTTCT+TGG + chr2_2:1368391-1368410 Msa0229600:CDS 30.0%
GTTTATGTATCTCACTTCTT+GGG + chr2_2:1368392-1368411 Msa0229600:CDS 30.0%
! ATCCGTCATATGATGTTTTA+TGG + chr2_2:1368453-1368472 Msa0229600:CDS 30.0%
TACCAACCATATTTACAATG+AGG - chr2_2:1368549-1368568 None:intergenic 30.0%
! GAACGTTTATCGACTAAAAT+AGG + chr2_2:1368727-1368746 Msa0229600:CDS 30.0%
!! TGTCTTTTCTTCTAAGAAAC+TGG + chr2_2:1368848-1368867 Msa0229600:intron 30.0%
AACAAAATACGGGTAATAAC+AGG - chr2_2:1368929-1368948 None:intergenic 30.0%
! TTTTAATCTACAAATGGCGA+CGG + chr2_2:1369118-1369137 Msa0229600:exon 30.0%
!! CTTTGTAAGAGTACCTTATA+GGG - chr2_2:1369203-1369222 None:intergenic 30.0%
!! TCTTTGTAAGAGTACCTTAT+AGG - chr2_2:1369204-1369223 None:intergenic 30.0%
GAACAATTCAAGACAAAACT+AGG + chr2_2:1369240-1369259 Msa0229600:three_prime_UTR 30.0%
TCTTGTAGCAGTCAAATTAT+TGG + chr2_2:1369311-1369330 Msa0229600:three_prime_UTR 30.0%
ATTGACAAAATCTTGATCAC+TGG - chr2_2:1369349-1369368 None:intergenic 30.0%
!!! TATTTTTCGAGTGATCCATT+TGG - chr2_2:1369471-1369490 None:intergenic 30.0%
ACTCAAGGAATAATTCTCTT+TGG - chr2_2:1369508-1369527 None:intergenic 30.0%
CTAATTAGCAACAAAGTGTA+TGG + chr2_2:1367855-1367874 Msa0229600:CDS 30.0%
AATTAGCAACAAAGTGTATG+GGG + chr2_2:1367857-1367876 Msa0229600:CDS 30.0%
GATTGTTGTCTTCACATAAT+AGG - chr2_2:1367986-1368005 None:intergenic 30.0%
! ATTGCACATAGTATTTTCCA+TGG - chr2_2:1368040-1368059 None:intergenic 30.0%
TAATTTCACCATACGACTAT+TGG + chr2_2:1368073-1368092 Msa0229600:CDS 30.0%
ATTGAAATCCAATAGTCGTA+TGG - chr2_2:1368084-1368103 None:intergenic 30.0%
TAAAGAATAATATGGAGGGA+TGG - chr2_2:1368126-1368145 None:intergenic 30.0%
ATCCCTCCATATTATTCTTT+AGG + chr2_2:1368125-1368144 Msa0229600:CDS 30.0%
AACATAGGTTTCGTCAATAT+AGG - chr2_2:1368224-1368243 None:intergenic 30.0%
TCTTTCTATGTTACTAGCTA+TGG + chr2_2:1368324-1368343 Msa0229600:intron 30.0%
TAGCTATGGTAAATCTCTAT+CGG + chr2_2:1368338-1368357 Msa0229600:intron 30.0%
AGTTTATGTATCTCACTTCT+TGG + chr2_2:1368391-1368410 Msa0229600:CDS 30.0%
GTTTATGTATCTCACTTCTT+GGG + chr2_2:1368392-1368411 Msa0229600:CDS 30.0%
! ATCCGTCATATGATGTTTTA+TGG + chr2_2:1368453-1368472 Msa0229600:CDS 30.0%
TACCAACCATATTTACAATG+AGG - chr2_2:1368549-1368568 None:intergenic 30.0%
! GAACGTTTATCGACTAAAAT+AGG + chr2_2:1368727-1368746 Msa0229600:CDS 30.0%
!! TGTCTTTTCTTCTAAGAAAC+TGG + chr2_2:1368848-1368867 Msa0229600:intron 30.0%
AACAAAATACGGGTAATAAC+AGG - chr2_2:1368929-1368948 None:intergenic 30.0%
! TTTTAATCTACAAATGGCGA+CGG + chr2_2:1369118-1369137 Msa0229600:exon 30.0%
!! CTTTGTAAGAGTACCTTATA+GGG - chr2_2:1369203-1369222 None:intergenic 30.0%
!! TCTTTGTAAGAGTACCTTAT+AGG - chr2_2:1369204-1369223 None:intergenic 30.0%
GAACAATTCAAGACAAAACT+AGG + chr2_2:1369240-1369259 Msa0229600:three_prime_UTR 30.0%
TCTTGTAGCAGTCAAATTAT+TGG + chr2_2:1369311-1369330 Msa0229600:three_prime_UTR 30.0%
ATTGACAAAATCTTGATCAC+TGG - chr2_2:1369349-1369368 None:intergenic 30.0%
!!! TATTTTTCGAGTGATCCATT+TGG - chr2_2:1369471-1369490 None:intergenic 30.0%
ACTCAAGGAATAATTCTCTT+TGG - chr2_2:1369508-1369527 None:intergenic 30.0%
TTCCTTAACGTCCTTTGTTA+TGG + chr2_2:1367818-1367837 Msa0229600:CDS 35.0%
TCGAGATAATAGAGTAGGTA+GGG - chr2_2:1368185-1368204 None:intergenic 35.0%
CTTTGTCGAGATAATAGAGT+AGG - chr2_2:1368190-1368209 None:intergenic 35.0%
AGGTTTCGTCAATATAGGAT+CGG - chr2_2:1368219-1368238 None:intergenic 35.0%
TTGAACAGCTCACATAACAT+AGG - chr2_2:1368239-1368258 None:intergenic 35.0%
CGCATGGAATATTCATACAT+TGG + chr2_2:1368261-1368280 Msa0229600:CDS 35.0%
GAATAAAACTCCATACTCAC+AGG + chr2_2:1368293-1368312 Msa0229600:intron 35.0%
GAATTTACATCCTGTGAGTA+TGG - chr2_2:1368306-1368325 None:intergenic 35.0%
GGTAAATCTCTATCGGAATT+CGG + chr2_2:1368345-1368364 Msa0229600:CDS 35.0%
GTAAATCTCTATCGGAATTC+GGG + chr2_2:1368346-1368365 Msa0229600:CDS 35.0%
TTTGAACTTTCTTGGCTCAA+TGG + chr2_2:1368477-1368496 Msa0229600:CDS 35.0%
CTCAATGGTCTTTGTAAACA+TGG + chr2_2:1368492-1368511 Msa0229600:CDS 35.0%
ATGGTCTTTGTAAACATGGT+CGG + chr2_2:1368496-1368515 Msa0229600:CDS 35.0%
AACCAGCCTCATTGTAAATA+TGG + chr2_2:1368540-1368559 Msa0229600:CDS 35.0%
AACCATATTTACAATGAGGC+TGG - chr2_2:1368545-1368564 None:intergenic 35.0%
AGCCTCATTGTAAATATGGT+TGG + chr2_2:1368544-1368563 Msa0229600:intron 35.0%
GTTAATTAGTAGCACTCACT+GGG - chr2_2:1368637-1368656 None:intergenic 35.0%
ATGTTCTTGCAGATTACTAC+TGG + chr2_2:1368683-1368702 Msa0229600:intron 35.0%
TGTTCTTGCAGATTACTACT+GGG + chr2_2:1368684-1368703 Msa0229600:intron 35.0%
AGATTACTACTGGGATAATG+TGG + chr2_2:1368693-1368712 Msa0229600:intron 35.0%
!!! GCATTGGTTGTTTTTCTTAG+CGG + chr2_2:1369058-1369077 Msa0229600:CDS 35.0%
! AGCGGTGTTAAATTTGTTCT+TGG + chr2_2:1369076-1369095 Msa0229600:CDS 35.0%
ACAATAGCATCATCCCTATA+AGG + chr2_2:1369187-1369206 Msa0229600:three_prime_UTR 35.0%
TTCAAGACAAAACTAGGCAA+TGG + chr2_2:1369246-1369265 Msa0229600:three_prime_UTR 35.0%
! TCGAAGTTGTCCTTTGAAAA+CGG - chr2_2:1369283-1369302 None:intergenic 35.0%
AAAATCTTGATCACTGGACT+TGG - chr2_2:1369343-1369362 None:intergenic 35.0%
TAGGATTCACCATGTTAATG+TGG + chr2_2:1369386-1369405 Msa0229600:three_prime_UTR 35.0%
TTCCTTAACGTCCTTTGTTA+TGG + chr2_2:1367818-1367837 Msa0229600:CDS 35.0%
TCGAGATAATAGAGTAGGTA+GGG - chr2_2:1368185-1368204 None:intergenic 35.0%
CTTTGTCGAGATAATAGAGT+AGG - chr2_2:1368190-1368209 None:intergenic 35.0%
AGGTTTCGTCAATATAGGAT+CGG - chr2_2:1368219-1368238 None:intergenic 35.0%
TTGAACAGCTCACATAACAT+AGG - chr2_2:1368239-1368258 None:intergenic 35.0%
CGCATGGAATATTCATACAT+TGG + chr2_2:1368261-1368280 Msa0229600:CDS 35.0%
GAATAAAACTCCATACTCAC+AGG + chr2_2:1368293-1368312 Msa0229600:intron 35.0%
GAATTTACATCCTGTGAGTA+TGG - chr2_2:1368306-1368325 None:intergenic 35.0%
GGTAAATCTCTATCGGAATT+CGG + chr2_2:1368345-1368364 Msa0229600:CDS 35.0%
GTAAATCTCTATCGGAATTC+GGG + chr2_2:1368346-1368365 Msa0229600:CDS 35.0%
TTTGAACTTTCTTGGCTCAA+TGG + chr2_2:1368477-1368496 Msa0229600:CDS 35.0%
CTCAATGGTCTTTGTAAACA+TGG + chr2_2:1368492-1368511 Msa0229600:CDS 35.0%
ATGGTCTTTGTAAACATGGT+CGG + chr2_2:1368496-1368515 Msa0229600:CDS 35.0%
AACCAGCCTCATTGTAAATA+TGG + chr2_2:1368540-1368559 Msa0229600:CDS 35.0%
AACCATATTTACAATGAGGC+TGG - chr2_2:1368545-1368564 None:intergenic 35.0%
AGCCTCATTGTAAATATGGT+TGG + chr2_2:1368544-1368563 Msa0229600:intron 35.0%
GTTAATTAGTAGCACTCACT+GGG - chr2_2:1368637-1368656 None:intergenic 35.0%
ATGTTCTTGCAGATTACTAC+TGG + chr2_2:1368683-1368702 Msa0229600:intron 35.0%
TGTTCTTGCAGATTACTACT+GGG + chr2_2:1368684-1368703 Msa0229600:intron 35.0%
AGATTACTACTGGGATAATG+TGG + chr2_2:1368693-1368712 Msa0229600:intron 35.0%
!!! GCATTGGTTGTTTTTCTTAG+CGG + chr2_2:1369058-1369077 Msa0229600:CDS 35.0%
! AGCGGTGTTAAATTTGTTCT+TGG + chr2_2:1369076-1369095 Msa0229600:CDS 35.0%
ACAATAGCATCATCCCTATA+AGG + chr2_2:1369187-1369206 Msa0229600:three_prime_UTR 35.0%
TTCAAGACAAAACTAGGCAA+TGG + chr2_2:1369246-1369265 Msa0229600:three_prime_UTR 35.0%
! TCGAAGTTGTCCTTTGAAAA+CGG - chr2_2:1369283-1369302 None:intergenic 35.0%
AAAATCTTGATCACTGGACT+TGG - chr2_2:1369343-1369362 None:intergenic 35.0%
TAGGATTCACCATGTTAATG+TGG + chr2_2:1369386-1369405 Msa0229600:three_prime_UTR 35.0%
CACCATAACAAAGGACGTTA+AGG - chr2_2:1367823-1367842 None:intergenic 40.0%
! CTTAACGTCCTTTGTTATGG+TGG + chr2_2:1367821-1367840 Msa0229600:CDS 40.0%
GAGTCATGTTCAAGAAAGTG+TGG + chr2_2:1367891-1367910 Msa0229600:CDS 40.0%
! GACTGTCTTTTAGCCTGAAA+GGG - chr2_2:1367935-1367954 None:intergenic 40.0%
AAAGACAGTCCAGAAGAATG+TGG + chr2_2:1367945-1367964 Msa0229600:CDS 40.0%
TGGAGGGATGGAATTATTAG+AGG - chr2_2:1368114-1368133 None:intergenic 40.0%
GAGATAATAGAGTAGGTAGG+GGG - chr2_2:1368183-1368202 None:intergenic 40.0%
CGAGATAATAGAGTAGGTAG+GGG - chr2_2:1368184-1368203 None:intergenic 40.0%
GTCGAGATAATAGAGTAGGT+AGG - chr2_2:1368186-1368205 None:intergenic 40.0%
CGTTAATTAGTAGCACTCAC+TGG - chr2_2:1368638-1368657 None:intergenic 40.0%
! TTTTCACTTTGCAGGTGCAT+TGG + chr2_2:1369042-1369061 Msa0229600:intron 40.0%
AAGACAAAACTAGGCAATGG+AGG + chr2_2:1369249-1369268 Msa0229600:three_prime_UTR 40.0%
AGACAAAACTAGGCAATGGA+GGG + chr2_2:1369250-1369269 Msa0229600:three_prime_UTR 40.0%
TTCAAAGGACAACTTCGAAG+TGG + chr2_2:1369285-1369304 Msa0229600:three_prime_UTR 40.0%
GTCAATGAAGTTGCTAGCAT+TGG + chr2_2:1369363-1369382 Msa0229600:three_prime_UTR 40.0%
!! ATGAAGTTGCTAGCATTGGT+AGG + chr2_2:1369367-1369386 Msa0229600:three_prime_UTR 40.0%
!!! CTCATTGGTTTTTGTGTGGA+AGG + chr2_2:1369414-1369433 Msa0229600:three_prime_UTR 40.0%
ATACAGTTGTTCGCAACTCA+AGG - chr2_2:1369523-1369542 None:intergenic 40.0%
CACCATAACAAAGGACGTTA+AGG - chr2_2:1367823-1367842 None:intergenic 40.0%
! CTTAACGTCCTTTGTTATGG+TGG + chr2_2:1367821-1367840 Msa0229600:CDS 40.0%
GAGTCATGTTCAAGAAAGTG+TGG + chr2_2:1367891-1367910 Msa0229600:CDS 40.0%
! GACTGTCTTTTAGCCTGAAA+GGG - chr2_2:1367935-1367954 None:intergenic 40.0%
AAAGACAGTCCAGAAGAATG+TGG + chr2_2:1367945-1367964 Msa0229600:CDS 40.0%
TGGAGGGATGGAATTATTAG+AGG - chr2_2:1368114-1368133 None:intergenic 40.0%
GAGATAATAGAGTAGGTAGG+GGG - chr2_2:1368183-1368202 None:intergenic 40.0%
CGAGATAATAGAGTAGGTAG+GGG - chr2_2:1368184-1368203 None:intergenic 40.0%
GTCGAGATAATAGAGTAGGT+AGG - chr2_2:1368186-1368205 None:intergenic 40.0%
CGTTAATTAGTAGCACTCAC+TGG - chr2_2:1368638-1368657 None:intergenic 40.0%
! TTTTCACTTTGCAGGTGCAT+TGG + chr2_2:1369042-1369061 Msa0229600:intron 40.0%
AAGACAAAACTAGGCAATGG+AGG + chr2_2:1369249-1369268 Msa0229600:three_prime_UTR 40.0%
AGACAAAACTAGGCAATGGA+GGG + chr2_2:1369250-1369269 Msa0229600:three_prime_UTR 40.0%
TTCAAAGGACAACTTCGAAG+TGG + chr2_2:1369285-1369304 Msa0229600:three_prime_UTR 40.0%
GTCAATGAAGTTGCTAGCAT+TGG + chr2_2:1369363-1369382 Msa0229600:three_prime_UTR 40.0%
!! ATGAAGTTGCTAGCATTGGT+AGG + chr2_2:1369367-1369386 Msa0229600:three_prime_UTR 40.0%
!!! CTCATTGGTTTTTGTGTGGA+AGG + chr2_2:1369414-1369433 Msa0229600:three_prime_UTR 40.0%
ATACAGTTGTTCGCAACTCA+AGG - chr2_2:1369523-1369542 None:intergenic 40.0%
ATAACATCAGCCACCCTTTC+AGG + chr2_2:1367919-1367938 Msa0229600:CDS 45.0%
! TGTCTTTTAGCCTGAAAGGG+TGG - chr2_2:1367932-1367951 None:intergenic 45.0%
! GGACTGTCTTTTAGCCTGAA+AGG - chr2_2:1367936-1367955 None:intergenic 45.0%
CTCTTGTCACCACATTCTTC+TGG - chr2_2:1367957-1367976 None:intergenic 45.0%
CAGAAGAATGTGGTGACAAG+AGG + chr2_2:1367955-1367974 Msa0229600:CDS 45.0%
ATAATAGAGTAGGTAGGGGG+AGG - chr2_2:1368180-1368199 None:intergenic 45.0%
TGTGAGCTGTTCAAATCGCA+TGG + chr2_2:1368245-1368264 Msa0229600:CDS 45.0%
ACTACTGGGATAATGTGGCT+TGG + chr2_2:1368698-1368717 Msa0229600:CDS 45.0%
AAACTAGGCAATGGAGGGTA+TGG + chr2_2:1369255-1369274 Msa0229600:three_prime_UTR 45.0%
! GGGTATGGATCCGTTTTCAA+AGG + chr2_2:1369270-1369289 Msa0229600:three_prime_UTR 45.0%
TGAGCTGCACCACATTAACA+TGG - chr2_2:1369398-1369417 None:intergenic 45.0%
GTTAATGTGGTGCAGCTCAT+TGG + chr2_2:1369399-1369418 Msa0229600:three_prime_UTR 45.0%
!!! GCAGCTCATTGGTTTTTGTG+TGG + chr2_2:1369410-1369429 Msa0229600:three_prime_UTR 45.0%
ATAACATCAGCCACCCTTTC+AGG + chr2_2:1367919-1367938 Msa0229600:CDS 45.0%
! TGTCTTTTAGCCTGAAAGGG+TGG - chr2_2:1367932-1367951 None:intergenic 45.0%
! GGACTGTCTTTTAGCCTGAA+AGG - chr2_2:1367936-1367955 None:intergenic 45.0%
CTCTTGTCACCACATTCTTC+TGG - chr2_2:1367957-1367976 None:intergenic 45.0%
CAGAAGAATGTGGTGACAAG+AGG + chr2_2:1367955-1367974 Msa0229600:CDS 45.0%
ATAATAGAGTAGGTAGGGGG+AGG - chr2_2:1368180-1368199 None:intergenic 45.0%
TGTGAGCTGTTCAAATCGCA+TGG + chr2_2:1368245-1368264 Msa0229600:CDS 45.0%
ACTACTGGGATAATGTGGCT+TGG + chr2_2:1368698-1368717 Msa0229600:CDS 45.0%
AAACTAGGCAATGGAGGGTA+TGG + chr2_2:1369255-1369274 Msa0229600:three_prime_UTR 45.0%
! GGGTATGGATCCGTTTTCAA+AGG + chr2_2:1369270-1369289 Msa0229600:three_prime_UTR 45.0%
TGAGCTGCACCACATTAACA+TGG - chr2_2:1369398-1369417 None:intergenic 45.0%
GTTAATGTGGTGCAGCTCAT+TGG + chr2_2:1369399-1369418 Msa0229600:three_prime_UTR 45.0%
!!! GCAGCTCATTGGTTTTTGTG+TGG + chr2_2:1369410-1369429 Msa0229600:three_prime_UTR 45.0%
CACTGCTGCCACCATAACAA+AGG - chr2_2:1367832-1367851 None:intergenic 50.0%
ATCTCTATCGGAATTCGGGC+TGG + chr2_2:1368350-1368369 Msa0229600:CDS 50.0%
TCTCTATCGGAATTCGGGCT+GGG + chr2_2:1368351-1368370 Msa0229600:CDS 50.0%
TTAGTAGCACTCACTGGGTG+TGG - chr2_2:1368632-1368651 None:intergenic 50.0%
CACTGCTGCCACCATAACAA+AGG - chr2_2:1367832-1367851 None:intergenic 50.0%
ATCTCTATCGGAATTCGGGC+TGG + chr2_2:1368350-1368369 Msa0229600:CDS 50.0%
TCTCTATCGGAATTCGGGCT+GGG + chr2_2:1368351-1368370 Msa0229600:CDS 50.0%
TTAGTAGCACTCACTGGGTG+TGG - chr2_2:1368632-1368651 None:intergenic 50.0%
! TCGGAATTCGGGCTGGGAGA+TGG + chr2_2:1368357-1368376 Msa0229600:CDS 60.0%
! CGGAATTCGGGCTGGGAGAT+GGG + chr2_2:1368358-1368377 Msa0229600:CDS 60.0%
! TCGGAATTCGGGCTGGGAGA+TGG + chr2_2:1368357-1368376 Msa0229600:CDS 60.0%
! CGGAATTCGGGCTGGGAGAT+GGG + chr2_2:1368358-1368377 Msa0229600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 1367813 1369550 1367813 ID=Msa0229600;Name=Msa0229600
chr2_2 mRNA 1367813 1369550 1367813 ID=Msa0229600-mRNA-1;Parent=Msa0229600;Name=Msa0229600-mRNA-1;_AED=0.05;_eAED=0.08;_QI=0|0.66|0.5|1|1|1|4|427|270
chr2_2 exon 1367813 1368282 1367813 ID=Msa0229600-mRNA-1:exon:438;Parent=Msa0229600-mRNA-1
chr2_2 exon 1368341 1368561 1368341 ID=Msa0229600-mRNA-1:exon:439;Parent=Msa0229600-mRNA-1
chr2_2 exon 1368695 1368748 1368695 ID=Msa0229600-mRNA-1:exon:440;Parent=Msa0229600-mRNA-1
chr2_2 exon 1369056 1369550 1369056 ID=Msa0229600-mRNA-1:exon:441;Parent=Msa0229600-mRNA-1
chr2_2 CDS 1367813 1368282 1367813 ID=Msa0229600-mRNA-1:cds;Parent=Msa0229600-mRNA-1
chr2_2 CDS 1368341 1368561 1368341 ID=Msa0229600-mRNA-1:cds;Parent=Msa0229600-mRNA-1
chr2_2 CDS 1368695 1368748 1368695 ID=Msa0229600-mRNA-1:cds;Parent=Msa0229600-mRNA-1
chr2_2 CDS 1369056 1369123 1369056 ID=Msa0229600-mRNA-1:cds;Parent=Msa0229600-mRNA-1
chr2_2 three_prime_UTR 1369124 1369550 1369124 ID=Msa0229600-mRNA-1:three_prime_utr;Parent=Msa0229600-mRNA-1
Gene Sequence

>Msa0229600

ATGCTTTCCTTAACGTCCTTTGTTATGGTGGCAGCAGTGATACTAATTAGCAACAAAGTGTATGGGGATGAGCAAAAAGAGTCATGTTCAAGAAAGTGTGGTGTTCATAACATCAGCCACCCTTTCAGGCTAAAAGACAGTCCAGAAGAATGTGGTGACAAGAGGTACAACCTATTATGTGAAGACAACAATCAATTGATTTTGTACTATGAATTTGAAGAATACCATGGAAAATACTATGTGCAATCAATTAATTACAATAATTTCACCATACGACTATTGGATTTCAATCTTGCTTCCTCTAATAATTCCATCCCTCCATATTATTCTTTAGGAATCTATAATTTCAGTTCTAACTACATCACCTCCCCCTACCTACTCTATTATCTCGACAAAGACTTCACCGATCCTATATTGACGAAACCTATGTTATGTGAGCTGTTCAAATCGCATGGAATATTCATACATTGCTATGGTAAATCTCTATCGGAATTCGGGCTGGGAGATGGGTGTCGTATACAGTTTATGTATCTCACTTCTTGGGATATTGATGATGACATAAATAACATTTTTTGCTCGGACATCCGTCATATGATGTTTTATGGATTTGAACTTTCTTGGCTCAATGGTCTTTGTAAACATGGTCGGTATGCAGTGCTCGATCAATATAACCAGCCTCATTGTAAATATGATTACTACTGGGATAATGTGGCTTGGACAGTTGAACGTTTATCGACTAAAATAGGTGCATTGGTTGTTTTTCTTAGCGGTGTTAAATTTGTTCTTGGAGCGCCATTGATCATTGTATTTTAA
Protein sequence

>Msa0229600

MLSLTSFVMVAAVILISNKVYGDEQKESCSRKCGVHNISHPFRLKDSPEECGDKRYNLLCEDNNQLILYYEFEEYHGKYYVQSINYNNFTIRLLDFNLASSNNSIPPYYSLGIYNFSSNYITSPYLLYYLDKDFTDPILTKPMLCELFKSHGIFIHCYGKSLSEFGLGDGCRIQFMYLTSWDIDDDINNIFCSDIRHMMFYGFELSWLNGLCKHGRYAVLDQYNQPHCKYDYYWDNVAWTVERLSTKIGALVVFLSGVKFVLGAPLIIVF*