AlfalfaGEDB | Msa0239870

M. sativa cultivar zhongmu-4 / Msa0239870

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0239870	ABN08857.1	98.630	146	2	0	1	146	1	146	6.83e-99	290

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0239870	A2Q5F7	98.630	146	2	0	1	146	1	146	3.26e-99	290

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0239840	Msa0239870	0.941967	1.649784e-101	-8.615850e-47
Msa0239870	Msa0317540	0.913620	4.982038e-84	-8.615850e-47
Msa0239870	Msa0791240	0.808920	2.442874e-50	-8.615850e-47
Msa0239870	Msa1144310	0.918777	1.024862e-86	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0239870	MtrunA17_Chr2g0290931	98.630	146	2	0	1	146	1	146	6.28e-103	290

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 24 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCTCCATTGATCCCTT+TGG	0.386929	2_2:-14459840	None:intergenic
AAGAATGAAGTGGAAGAGAA+AGG	0.519180	2_2:+14459633	Msa0239870:CDS
ACTTGTTTCAAGGTTTCTTC+TGG	0.404656	2_2:+14459777	Msa0239870:CDS
AGATTGTGTGAGGCTCCAAA+GGG	0.596314	2_2:+14459825	Msa0239870:CDS
AGGGATCAATGGAGGATTTG+TGG	0.464367	2_2:+14459844	Msa0239870:CDS
ATGACACCATCTTTCAACCA+AGG	0.654692	2_2:-14459684	None:intergenic
ATGATGTTGAAGCAAAGATT+TGG	0.461294	2_2:+14459541	Msa0239870:CDS
ATGTGGCACAAGTTTCACCA+AGG	0.735233	2_2:+14459608	Msa0239870:CDS
CACCAAGGAAAAGAATGAAG+TGG	0.603149	2_2:+14459623	Msa0239870:CDS
CCAAGACAACACTTGTTTCA+AGG	0.366636	2_2:+14459767	Msa0239870:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATGTTGAAGCAAAGATT+TGG	+	chr2_2:14459541-14459560	Msa0239870:CDS	30.0%
GCTAAAAAAAGAGATGTATG+TGG	+	chr2_2:14459591-14459610	Msa0239870:CDS	30.0%
TCCAATCTATTAACCTCAAA+TGG	-	chr2_2:14459873-14459892	None:intergenic	30.0%
AAGAATGAAGTGGAAGAGAA+AGG	+	chr2_2:14459633-14459652	Msa0239870:CDS	35.0%
GAGAAAGGTTGTTTAGCATT+TGG	+	chr2_2:14459648-14459667	Msa0239870:CDS	35.0%
ACTTGTTTCAAGGTTTCTTC+TGG	+	chr2_2:14459777-14459796	Msa0239870:CDS	35.0%
GGAAAATGATAGATTGTGTG+AGG	+	chr2_2:14459815-14459834	Msa0239870:CDS	35.0%
CACCAAGGAAAAGAATGAAG+TGG	+	chr2_2:14459623-14459642	Msa0239870:CDS	40.0%
GATTTCCCTTGGTTGAAAGA+TGG	+	chr2_2:14459678-14459697	Msa0239870:CDS	40.0%
TGACACCATCTTTCAACCAA+GGG	-	chr2_2:14459686-14459705	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	14459504	14459944	14459504	ID=Msa0239870;Name=Msa0239870
chr2_2	mRNA	14459504	14459944	14459504	ID=Msa0239870-mRNA-1;Parent=Msa0239870;Name=Msa0239870-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|146
chr2_2	exon	14459504	14459944	14459504	ID=Msa0239870-mRNA-1:exon:5999;Parent=Msa0239870-mRNA-1
chr2_2	CDS	14459504	14459944	14459504	ID=Msa0239870-mRNA-1:cds;Parent=Msa0239870-mRNA-1

Gene Sequence

>Msa0239870

ATGGAGCAAGAGAAGAATAAGAGAGCAAGAGAATATGATGATGTTGAAGCAAAGATTTGGTTTTCTTTTGAGAATAATAACAAGAAAGCTAAAAAAAGAGATGTATGTGGCACAAGTTTCACCAAGGAAAAGAATGAAGTGGAAGAGAAAGGTTGTTTAGCATTTGGAGTGTTTGATTTCCCTTGGTTGAAAGATGGTGTCATATACAAATTAGAAGATTGTTTAATTGATTTTGAAGACAATTTCTTGTCATCATTACAAGACCAAGACAACACTTGTTTCAAGGTTTCTTCTGGTATTGATTTCTTTGAGGAAAATGATAGATTGTGTGAGGCTCCAAAGGGATCAATGGAGGATTTGTGGCTGCCATTTGAGGTTAATAGATTGGAGCTTGAAGCAGAAGATTTGGATTGTATTTGTAGCTCTTTGCTAAACAATTAA

Protein sequence

>Msa0239870

MEQEKNKRAREYDDVEAKIWFSFENNNKKAKKRDVCGTSFTKEKNEVEEKGCLAFGVFDFPWLKDGVIYKLEDCLIDFEDNFLSSLQDQDNTCFKVSSGIDFFEENDRLCEAPKGSMEDLWLPFEVNRLELEAEDLDCICSSLLNN*