Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791240 | ABN08857.1 | 98.630 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 6.83e-99 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791240 | A2Q5F7 | 98.630 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 3.26e-99 | 290 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005270 | Msa0791240 | 0.809635 | 1.716527e-50 | -8.615850e-47 |
| Msa0007440 | Msa0791240 | 0.807862 | 4.108258e-50 | -8.615850e-47 |
| Msa0014710 | Msa0791240 | 0.805229 | 1.476585e-49 | -8.615850e-47 |
| Msa0026660 | Msa0791240 | 0.805613 | 1.226428e-49 | -8.615850e-47 |
| Msa0037880 | Msa0791240 | 0.803658 | 3.138204e-49 | -8.615850e-47 |
| Msa0038870 | Msa0791240 | 0.825473 | 4.598961e-54 | -8.615850e-47 |
| Msa0040620 | Msa0791240 | 0.808948 | 2.409993e-50 | -8.615850e-47 |
| Msa0055100 | Msa0791240 | 0.813632 | 2.319827e-51 | -8.615850e-47 |
| Msa0056030 | Msa0791240 | 0.808920 | 2.442924e-50 | -8.615850e-47 |
| Msa0073700 | Msa0791240 | 0.807348 | 5.281403e-50 | -8.615850e-47 |
| Msa0086420 | Msa0791240 | 0.833719 | 4.538287e-56 | -8.615850e-47 |
| Msa0096980 | Msa0791240 | 0.806487 | 8.031033e-50 | -8.615850e-47 |
| Msa0097590 | Msa0791240 | 0.810819 | 9.537598e-51 | -8.615850e-47 |
| Msa0097610 | Msa0791240 | 0.803334 | 3.663041e-49 | -8.615850e-47 |
| Msa0101180 | Msa0791240 | 0.802546 | 5.329149e-49 | -8.615850e-47 |
| Msa0104800 | Msa0791240 | 0.820937 | 5.268634e-53 | -8.615850e-47 |
| Msa0112770 | Msa0791240 | 0.804101 | 2.539207e-49 | -8.615850e-47 |
| Msa0621650 | Msa0791240 | 0.803332 | 3.666174e-49 | -8.615850e-47 |
| Msa0638070 | Msa0791240 | 0.817400 | 3.363892e-52 | -8.615850e-47 |
| Msa0641730 | Msa0791240 | 0.806318 | 8.717878e-50 | -8.615850e-47 |
| Msa0643320 | Msa0791240 | 0.801530 | 8.622071e-49 | -8.615850e-47 |
| Msa0644660 | Msa0791240 | 0.807148 | 5.823856e-50 | -8.615850e-47 |
| Msa0669720 | Msa0791240 | 0.809514 | 1.822394e-50 | -8.615850e-47 |
| Msa0679550 | Msa0791240 | 0.805487 | 1.303698e-49 | -8.615850e-47 |
| Msa0683140 | Msa0791240 | 0.806582 | 7.670390e-50 | -8.615850e-47 |
| Msa0705190 | Msa0791240 | 0.820426 | 6.902114e-53 | -8.615850e-47 |
| Msa0709160 | Msa0791240 | 0.804663 | 1.938597e-49 | -8.615850e-47 |
| Msa0711100 | Msa0791240 | 0.801401 | 9.164316e-49 | -8.615850e-47 |
| Msa0721280 | Msa0791240 | 0.836536 | 8.834055e-57 | -8.615850e-47 |
| Msa0732600 | Msa0791240 | 0.809156 | 2.174921e-50 | -8.615850e-47 |
| Msa0743530 | Msa0791240 | 0.802265 | 6.088717e-49 | -8.615850e-47 |
| Msa0763890 | Msa0791240 | 0.803083 | 4.127962e-49 | -8.615850e-47 |
| Msa0763920 | Msa0791240 | 0.805300 | 1.426844e-49 | -8.615850e-47 |
| Msa0773590 | Msa0791240 | 0.806993 | 6.280682e-50 | -8.615850e-47 |
| Msa0774750 | Msa0791240 | 0.803586 | 3.248648e-49 | -8.615850e-47 |
| Msa0784380 | Msa0791240 | 0.803113 | 4.069904e-49 | -8.615850e-47 |
| Msa0791240 | Msa0811040 | 0.810586 | 1.070966e-50 | -8.615850e-47 |
| Msa0791240 | Msa0812140 | 0.801823 | 7.507048e-49 | -8.615850e-47 |
| Msa0791240 | Msa0838260 | 0.800903 | 1.158543e-48 | -8.615850e-47 |
| Msa0791240 | Msa0844110 | 0.802968 | 4.361953e-49 | -8.615850e-47 |
| Msa0791240 | Msa0861470 | 0.802034 | 6.792651e-49 | -8.615850e-47 |
| Msa0791240 | Msa0861570 | 0.804546 | 2.051226e-49 | -8.615850e-47 |
| Msa0791240 | Msa0866400 | 0.823254 | 1.529149e-53 | -8.615850e-47 |
| Msa0791240 | Msa0866410 | 0.821409 | 4.100591e-53 | -8.615850e-47 |
| Msa0791240 | Msa0909010 | 0.800714 | 1.266195e-48 | -8.615850e-47 |
| Msa0791240 | Msa0916390 | 0.811505 | 6.768951e-51 | -8.615850e-47 |
| Msa0791240 | Msa0923780 | 0.804475 | 2.121777e-49 | -8.615850e-47 |
| Msa0791240 | Msa0937330 | 0.807008 | 6.235688e-50 | -8.615850e-47 |
| Msa0791240 | Msa0962840 | 0.802719 | 4.909263e-49 | -8.615850e-47 |
| Msa0791240 | Msa0965020 | 0.802006 | 6.883933e-49 | -8.615850e-47 |
| Msa0791240 | Msa0994370 | 0.809446 | 1.884357e-50 | -8.615850e-47 |
| Msa0791240 | Msa1009750 | 0.807956 | 3.923960e-50 | -8.615850e-47 |
| Msa0791240 | Msa1014420 | 0.800139 | 1.657710e-48 | -8.615850e-47 |
| Msa0791240 | Msa1027250 | 0.801317 | 9.533370e-49 | -8.615850e-47 |
| Msa0791240 | Msa1027360 | 0.804016 | 2.644551e-49 | -8.615850e-47 |
| Msa0791240 | Msa1031770 | 0.800154 | 1.646393e-48 | -8.615850e-47 |
| Msa0791240 | Msa1063420 | 0.811163 | 8.033316e-51 | -8.615850e-47 |
| Msa0791240 | Msa1068870 | 0.803832 | 2.887562e-49 | -8.615850e-47 |
| Msa0791240 | Msa1071320 | 0.807194 | 5.694872e-50 | -8.615850e-47 |
| Msa0791240 | Msa1097820 | 0.809125 | 2.208163e-50 | -8.615850e-47 |
| Msa0791240 | Msa1113590 | 0.806381 | 8.455011e-50 | -8.615850e-47 |
| Msa0791240 | Msa1125550 | 0.801752 | 7.764969e-49 | -8.615850e-47 |
| Msa0791240 | Msa1130530 | 0.810588 | 1.069628e-50 | -8.615850e-47 |
| Msa0791240 | Msa1144310 | 0.846676 | 1.869982e-59 | -8.615850e-47 |
| Msa0791240 | Msa1144970 | 0.803918 | 2.771352e-49 | -8.615850e-47 |
| Msa0791240 | Msa1157740 | 0.804074 | 2.572421e-49 | -8.615850e-47 |
| Msa0791240 | Msa1158770 | 0.814875 | 1.232660e-51 | -8.615850e-47 |
| Msa0791240 | Msa1167010 | 0.820381 | 7.067482e-53 | -8.615850e-47 |
| Msa0791240 | Msa1173900 | 0.815629 | 8.382310e-52 | -8.615850e-47 |
| Msa0791240 | Msa1174850 | 0.802093 | 6.606869e-49 | -8.615850e-47 |
| Msa0791240 | Msa1176360 | 0.801776 | 7.676749e-49 | -8.615850e-47 |
| Msa0791240 | Msa1178060 | 0.802829 | 4.658909e-49 | -8.615850e-47 |
| Msa0791240 | Msa1178370 | 0.803700 | 3.076337e-49 | -8.615850e-47 |
| Msa0791240 | Msa1188600 | -0.810439 | 1.152342e-50 | -8.615850e-47 |
| Msa0791240 | Msa1211830 | 0.804220 | 2.397932e-49 | -8.615850e-47 |
| Msa0791240 | Msa1237980 | 0.815110 | 1.093550e-51 | -8.615850e-47 |
| Msa0791240 | Msa1242580 | -0.804240 | 2.375087e-49 | -8.615850e-47 |
| Msa0791240 | Msa1270660 | 0.829926 | 3.915263e-55 | -8.615850e-47 |
| Msa0791240 | Msa1299700 | 0.805935 | 1.049804e-49 | -8.615850e-47 |
| Msa0791240 | Msa1332050 | 0.804941 | 1.696450e-49 | -8.615850e-47 |
| Msa0791240 | Msa1332220 | 0.800432 | 1.445392e-48 | -8.615850e-47 |
| Msa0791240 | Msa1337170 | 0.800102 | 1.686668e-48 | -8.615850e-47 |
| Msa0791240 | Msa1341490 | 0.813153 | 2.956443e-51 | -8.615850e-47 |
| Msa0791240 | Msa1357890 | 0.833554 | 4.988043e-56 | -8.615850e-47 |
| Msa0791240 | Msa1388860 | 0.813156 | 2.952469e-51 | -8.615850e-47 |
| Msa0791240 | Msa1398090 | 0.833989 | 3.883863e-56 | -8.615850e-47 |
| Msa0791240 | Msa1401420 | 0.806869 | 6.670064e-50 | -8.615850e-47 |
| Msa0791240 | Msa1416370 | 0.810409 | 1.169459e-50 | -8.615850e-47 |
| Msa0791240 | Msa1417810 | -0.802850 | 4.613417e-49 | -8.615850e-47 |
| Msa0791240 | Msa1422810 | 0.804044 | 2.609372e-49 | -8.615850e-47 |
| Msa0791240 | Msa1426570 | 0.812498 | 4.113474e-51 | -8.615850e-47 |
| Msa0791240 | Msa1433600 | 0.808726 | 2.688274e-50 | -8.615850e-47 |
| Msa0791240 | Msa1442700 | 0.818636 | 1.767260e-52 | -8.615850e-47 |
| Msa0791240 | Msa1445400 | -0.802468 | 5.529822e-49 | -8.615850e-47 |
| Msa0791240 | Msa1449350 | -0.804620 | 1.979704e-49 | -8.615850e-47 |
| Msa0791240 | Msa1454680 | 0.805977 | 1.028703e-49 | -8.615850e-47 |
| Msa0791240 | Msa1465570 | 0.820056 | 8.388634e-53 | -8.615850e-47 |
| Msa0135420 | Msa0791240 | 0.809607 | 1.740682e-50 | -8.615850e-47 |
| Msa0138500 | Msa0791240 | 0.810670 | 1.027129e-50 | -8.615850e-47 |
| Msa0138520 | Msa0791240 | 0.810390 | 1.180753e-50 | -8.615850e-47 |
| Msa0140510 | Msa0791240 | -0.801013 | 1.100197e-48 | -8.615850e-47 |
| Msa0142950 | Msa0791240 | 0.803436 | 3.488996e-49 | -8.615850e-47 |
| Msa0145010 | Msa0791240 | 0.806373 | 8.488636e-50 | -8.615850e-47 |
| Msa0145150 | Msa0791240 | 0.819803 | 9.588961e-53 | -8.615850e-47 |
| Msa0156700 | Msa0791240 | 0.818137 | 2.292983e-52 | -8.615850e-47 |
| Msa0197610 | Msa0791240 | 0.804757 | 1.852845e-49 | -8.615850e-47 |
| Msa0233380 | Msa0791240 | 0.807076 | 6.031951e-50 | -8.615850e-47 |
| Msa0365860 | Msa0791240 | 0.803779 | 2.961978e-49 | -8.615850e-47 |
| Msa0376280 | Msa0791240 | 0.800067 | 1.714464e-48 | -8.615850e-47 |
| Msa0381000 | Msa0791240 | 0.802558 | 5.298922e-49 | -8.615850e-47 |
| Msa0430870 | Msa0791240 | 0.814035 | 1.891156e-51 | -8.615850e-47 |
| Msa0430950 | Msa0791240 | 0.800231 | 1.587972e-48 | -8.615850e-47 |
| Msa0442270 | Msa0791240 | 0.807008 | 6.234438e-50 | -8.615850e-47 |
| Msa0457510 | Msa0791240 | 0.800519 | 1.387186e-48 | -8.615850e-47 |
| Msa0239840 | Msa0791240 | 0.829378 | 5.322694e-55 | -8.615850e-47 |
| Msa0239870 | Msa0791240 | 0.808920 | 2.442874e-50 | -8.615850e-47 |
| Msa0255080 | Msa0791240 | 0.814036 | 1.890008e-51 | -8.615850e-47 |
| Msa0259370 | Msa0791240 | 0.811801 | 5.838788e-51 | -8.615850e-47 |
| Msa0317540 | Msa0791240 | 0.838831 | 2.276077e-57 | -8.615850e-47 |
| Msa0339910 | Msa0791240 | 0.802742 | 4.856095e-49 | -8.615850e-47 |
| Msa0484780 | Msa0791240 | 0.801748 | 7.779389e-49 | -8.615850e-47 |
| Msa0489660 | Msa0791240 | 0.813813 | 2.116808e-51 | -8.615850e-47 |
| Msa0506830 | Msa0791240 | 0.802334 | 5.893639e-49 | -8.615850e-47 |
| Msa0524600 | Msa0791240 | 0.805950 | 1.042387e-49 | -8.615850e-47 |
| Msa0527910 | Msa0791240 | 0.809194 | 2.134652e-50 | -8.615850e-47 |
| Msa0534900 | Msa0791240 | 0.810895 | 9.180973e-51 | -8.615850e-47 |
| Msa0549780 | Msa0791240 | 0.840990 | 6.233506e-58 | -8.615850e-47 |
| Msa0571780 | Msa0791240 | 0.804453 | 2.144666e-49 | -8.615850e-47 |
| Msa0600080 | Msa0791240 | 0.801743 | 7.794672e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0791240 | MtrunA17_Chr2g0290931 | 98.630 | 146 | 2 | 0 | 1 | 146 | 1 | 146 | 6.28e-103 | 290 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGCTTGAAGCAGAAGATT+TGG | 0.301631 | 5_3:-2136975 | Msa0791240:CDS |
| GAGAAAGGTTGTTTAGCATT+TGG | 0.326273 | 5_3:-2137217 | Msa0791240:CDS |
| GCCATTTGAGGTTAATAGAT+TGG | 0.344754 | 5_3:-2136996 | Msa0791240:CDS |
| CCAAGACAACACTTGTTTCA+AGG | 0.366636 | 5_3:-2137098 | Msa0791240:CDS |
| AAATCCTCCATTGATCCCTT+TGG | 0.386929 | 5_3:+2137025 | None:intergenic |
| TTCTGGTATTGATTTCTTTG+AGG | 0.395501 | 5_3:-2137071 | Msa0791240:CDS |
| ACTTGTTTCAAGGTTTCTTC+TGG | 0.404656 | 5_3:-2137088 | Msa0791240:CDS |
| TCCAATCTATTAACCTCAAA+TGG | 0.414962 | 5_3:+2136995 | None:intergenic |
| CCTTGAAACAAGTGTTGTCT+TGG | 0.422034 | 5_3:+2137098 | None:intergenic |
| TTGGAGTGTTTGATTTCCCT+TGG | 0.432978 | 5_3:-2137198 | Msa0791240:CDS |
| ATGATGTTGAAGCAAAGATT+TGG | 0.461294 | 5_3:-2137324 | Msa0791240:CDS |
| AGGGATCAATGGAGGATTTG+TGG | 0.464367 | 5_3:-2137021 | Msa0791240:CDS |
| GGATTTGTGGCTGCCATTTG+AGG | 0.474098 | 5_3:-2137008 | Msa0791240:CDS |
| GATTTCCCTTGGTTGAAAGA+TGG | 0.486271 | 5_3:-2137187 | Msa0791240:CDS |
| TGAGGCTCCAAAGGGATCAA+TGG | 0.510645 | 5_3:-2137032 | Msa0791240:CDS |
| AAGAATGAAGTGGAAGAGAA+AGG | 0.519180 | 5_3:-2137232 | Msa0791240:CDS |
| TAGATTGTGTGAGGCTCCAA+AGG | 0.564320 | 5_3:-2137041 | Msa0791240:CDS |
| AGATTGTGTGAGGCTCCAAA+GGG | 0.596314 | 5_3:-2137040 | Msa0791240:CDS |
| CACCAAGGAAAAGAATGAAG+TGG | 0.603149 | 5_3:-2137242 | Msa0791240:CDS |
| TGACACCATCTTTCAACCAA+GGG | 0.626669 | 5_3:+2137182 | None:intergenic |
| ATGACACCATCTTTCAACCA+AGG | 0.654692 | 5_3:+2137181 | None:intergenic |
| GGCTCCAAAGGGATCAATGG+AGG | 0.655913 | 5_3:-2137029 | Msa0791240:CDS |
| GGAAAATGATAGATTGTGTG+AGG | 0.713281 | 5_3:-2137050 | Msa0791240:CDS |
| ATGTGGCACAAGTTTCACCA+AGG | 0.735233 | 5_3:-2137257 | Msa0791240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGATGTTGAAGCAAAGATT+TGG | - | chr5_3:2136980-2136999 | Msa0791240:CDS | 30.0% | |
| GCTAAAAAAAGAGATGTATG+TGG | - | chr5_3:2137030-2137049 | Msa0791240:CDS | 30.0% | |
| !! | TTCTGGTATTGATTTCTTTG+AGG | - | chr5_3:2137233-2137252 | Msa0791240:CDS | 30.0% |
| TCCAATCTATTAACCTCAAA+TGG | + | chr5_3:2137312-2137331 | None:intergenic | 30.0% | |
| ! | TTCCACTTCATTCTTTTCCT+TGG | + | chr5_3:2137067-2137086 | None:intergenic | 35.0% |
| AAGAATGAAGTGGAAGAGAA+AGG | - | chr5_3:2137072-2137091 | Msa0791240:CDS | 35.0% | |
| GAGAAAGGTTGTTTAGCATT+TGG | - | chr5_3:2137087-2137106 | Msa0791240:CDS | 35.0% | |
| ACTTGTTTCAAGGTTTCTTC+TGG | - | chr5_3:2137216-2137235 | Msa0791240:CDS | 35.0% | |
| GGAAAATGATAGATTGTGTG+AGG | - | chr5_3:2137254-2137273 | Msa0791240:CDS | 35.0% | |
| ! | GCCATTTGAGGTTAATAGAT+TGG | - | chr5_3:2137308-2137327 | Msa0791240:CDS | 35.0% |
| CACCAAGGAAAAGAATGAAG+TGG | - | chr5_3:2137062-2137081 | Msa0791240:CDS | 40.0% | |
| ! | TTGGAGTGTTTGATTTCCCT+TGG | - | chr5_3:2137106-2137125 | Msa0791240:CDS | 40.0% |
| GATTTCCCTTGGTTGAAAGA+TGG | - | chr5_3:2137117-2137136 | Msa0791240:CDS | 40.0% | |
| TGACACCATCTTTCAACCAA+GGG | + | chr5_3:2137125-2137144 | None:intergenic | 40.0% | |
| ATGACACCATCTTTCAACCA+AGG | + | chr5_3:2137126-2137145 | None:intergenic | 40.0% | |
| ! | CCTTGAAACAAGTGTTGTCT+TGG | + | chr5_3:2137209-2137228 | None:intergenic | 40.0% |
| CCAAGACAACACTTGTTTCA+AGG | - | chr5_3:2137206-2137225 | Msa0791240:CDS | 40.0% | |
| AAATCCTCCATTGATCCCTT+TGG | + | chr5_3:2137282-2137301 | None:intergenic | 40.0% | |
| ATGTGGCACAAGTTTCACCA+AGG | - | chr5_3:2137047-2137066 | Msa0791240:CDS | 45.0% | |
| TAGATTGTGTGAGGCTCCAA+AGG | - | chr5_3:2137263-2137282 | Msa0791240:CDS | 45.0% | |
| AGATTGTGTGAGGCTCCAAA+GGG | - | chr5_3:2137264-2137283 | Msa0791240:CDS | 45.0% | |
| AGGGATCAATGGAGGATTTG+TGG | - | chr5_3:2137283-2137302 | Msa0791240:CDS | 45.0% | |
| GGAGCTTGAAGCAGAAGATT+TGG | - | chr5_3:2137329-2137348 | Msa0791240:CDS | 45.0% | |
| TGAGGCTCCAAAGGGATCAA+TGG | - | chr5_3:2137272-2137291 | Msa0791240:CDS | 50.0% | |
| ! | GGATTTGTGGCTGCCATTTG+AGG | - | chr5_3:2137296-2137315 | Msa0791240:CDS | 50.0% |
| GGCTCCAAAGGGATCAATGG+AGG | - | chr5_3:2137275-2137294 | Msa0791240:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 2136943 | 2137383 | 2136943 | ID=Msa0791240;Name=Msa0791240 |
| chr5_3 | mRNA | 2136943 | 2137383 | 2136943 | ID=Msa0791240-mRNA-1;Parent=Msa0791240;Name=Msa0791240-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|146 |
| chr5_3 | exon | 2136943 | 2137383 | 2136943 | ID=Msa0791240-mRNA-1:exon:910;Parent=Msa0791240-mRNA-1 |
| chr5_3 | CDS | 2136943 | 2137383 | 2136943 | ID=Msa0791240-mRNA-1:cds;Parent=Msa0791240-mRNA-1 |
| Gene Sequence |
| Protein sequence |
