AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0246850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0246850 MtrunA17_Chr2g0301361 98.953 191 2 0 1 191 1 191 7.20e-138 394
Msa0246850 MtrunA17_Chr4g0070171 70.103 194 54 1 3 192 2 195 2.40e-83 255
Msa0246850 MtrunA17_Chr8g0388531 44.094 127 71 0 66 192 34 160 3.86e-34 120
Msa0246850 MtrunA17_Chr4g0052121 48.438 128 63 1 68 192 46 173 1.28e-33 124
Msa0246850 MtrunA17_Chr1g0204841 39.264 163 86 2 33 192 23 175 1.49e-33 124
Msa0246850 MtrunA17_Chr8g0388511 46.341 123 66 0 66 188 6 128 1.28e-32 120
Msa0246850 MtrunA17_Chr8g0388551 31.852 135 65 2 37 171 2 109 1.41e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0246850 AT1G74960.1 51.867 241 64 4 2 191 5 244 8.86e-70 221
Msa0246850 AT1G74960.3 51.867 241 64 4 2 191 5 244 8.86e-70 221
Msa0246850 AT1G74960.2 51.867 241 64 4 2 191 5 244 8.86e-70 221
Msa0246850 AT5G46290.2 38.854 157 94 1 36 192 22 176 1.97e-33 123
Msa0246850 AT5G46290.1 38.854 157 94 1 36 192 22 176 3.56e-33 124
Msa0246850 AT5G46290.3 38.854 157 94 1 36 192 22 176 4.28e-33 123

Find 77 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGGGAATTCAGTTATT+TGG 0.143098 2_2:+28576013 Msa0246850:three_prime_UTR
ATTCTCATTCATTCTCTTCA+TGG 0.192854 2_2:+28573324 Msa0246850:exon
CTCTGTTTCCAGAGGATTGC+TGG 0.218073 2_2:+28575496 Msa0246850:intron
CCCGAGACTCTCAGATTTCT+TGG 0.228465 2_2:+28576088 Msa0246850:three_prime_UTR
GATTGGGAGAAGAGAGATTA+AGG 0.255347 2_2:-28573462 None:intergenic
GCATAATAACATTCGTTTGT+AGG 0.328298 2_2:+28575805 Msa0246850:three_prime_UTR
GAATTCAGTTATTTGGATAA+AGG 0.343779 2_2:+28576020 Msa0246850:three_prime_UTR
TAAAGTAATGGCTGTAGCTT+TGG 0.350403 2_2:+28575223 Msa0246850:CDS
AATGAAGGAGAAAGGTTAAG+AGG 0.360333 2_2:-28573278 None:intergenic
TACCTGCATTCCACCCATTC+CGG 0.366985 2_2:-28575692 None:intergenic
AGTGGGATAAGCGAGATTAA+TGG 0.369472 2_2:+28575380 Msa0246850:CDS
ACTACTCGTCTTTGCTCTAT+AGG 0.387655 2_2:-28575281 None:intergenic
TCCATCCTCTTTGAAAACTT+TGG 0.397379 2_2:-28575556 None:intergenic
AGACCAATCTGTGTAATTCT+TGG 0.398020 2_2:+28576055 Msa0246850:three_prime_UTR
CCCAATCGAATGTCCCGTTC+TGG 0.406670 2_2:+28573478 Msa0246850:CDS
TTCTACAATAATCTACTTGA+TGG 0.427157 2_2:+28575353 Msa0246850:CDS
TATAAAGTAAACTGCTGAAT+GGG 0.433264 2_2:-28575772 None:intergenic
TCTGTTTCCAGAGGATTGCT+GGG 0.438070 2_2:+28575497 Msa0246850:intron
TTTGAGTGAGAGAAGAGAAC+TGG 0.439999 2_2:-28573204 None:intergenic
ATCGTTCTCGTCGTCGCAGC+AGG 0.440595 2_2:+28573431 Msa0246850:CDS
TAAAGATGCAGAGGAAAAGA+AGG 0.441085 2_2:+28575997 Msa0246850:three_prime_UTR
AGATTATTGTAGAAGACATC+TGG 0.441095 2_2:-28575344 None:intergenic
AAGTGTGAAATTACCAGAAC+GGG 0.446164 2_2:-28573491 None:intergenic
CGAGTAGTTGTGACGGGTAT+GGG 0.449622 2_2:+28575296 Msa0246850:CDS
CAGTTATTTGGATAAAGGAT+TGG 0.460625 2_2:+28576025 Msa0246850:three_prime_UTR
CCGAGACTCTCAGATTTCTT+GGG 0.461829 2_2:+28576089 Msa0246850:three_prime_UTR
ACGAGTAGTTGTGACGGGTA+TGG 0.463031 2_2:+28575295 Msa0246850:CDS
AATCTACTTGATGGTGTTAG+TGG 0.463552 2_2:+28575362 Msa0246850:CDS
AGATAACAGTTACTGCAGAT+TGG 0.473867 2_2:-28575933 None:intergenic
AAAGAAAGAATGAAGGAGAA+AGG 0.475352 2_2:-28573286 None:intergenic
ATGAAGAGAATGAATGAGAA+TGG 0.475541 2_2:-28573322 None:intergenic
TATTTGGATAAAGGATTGGT+AGG 0.478947 2_2:+28576029 Msa0246850:three_prime_UTR
ACCAGAACGGGACATTCGAT+TGG 0.480965 2_2:-28573479 None:intergenic
ATGTTGTATCTGCTGACAGC+TGG 0.481211 2_2:+28575586 Msa0246850:CDS
GTTCCAAGAATTACACAGAT+TGG 0.482500 2_2:-28576058 None:intergenic
AGAGATGAAAGAAAGAATGA+AGG 0.482672 2_2:-28573293 None:intergenic
ACGATGAGATTGAGAAGAGT+GGG 0.485286 2_2:-28573413 None:intergenic
ATCAAATCTTTCTCAACTGA+TGG 0.487001 2_2:+28575523 Msa0246850:CDS
CTCCGGAATGGGTGGAATGC+AGG 0.488440 2_2:+28575690 Msa0246850:CDS
CCAAGAAATCTGAGAGTCTC+GGG 0.492939 2_2:-28576088 None:intergenic
ATATAAAGTAAACTGCTGAA+TGG 0.499375 2_2:-28575773 None:intergenic
AATAACATTCGTTTGTAGGA+AGG 0.519715 2_2:+28575809 Msa0246850:three_prime_UTR
CCCAAGAAATCTGAGAGTCT+CGG 0.520233 2_2:-28576089 None:intergenic
CCAGAACGGGACATTCGATT+GGG 0.522885 2_2:-28573478 None:intergenic
ATGGGTGTGGTCACACCACT+TGG 0.525435 2_2:+28575314 Msa0246850:CDS
CGTGTCGGTATAATTACAAT+GGG 0.535143 2_2:-28576114 None:intergenic
TGTTAGTAATAAGCCACCTA+GGG 0.538444 2_2:+28575838 Msa0246850:three_prime_UTR
AAAATTTCAAGGTAAAGTAA+TGG 0.539854 2_2:+28575211 Msa0246850:intron
ATTTGGATAAAGGATTGGTA+GGG 0.542217 2_2:+28576030 Msa0246850:three_prime_UTR
TTGTTAGTAATAAGCCACCT+AGG 0.543502 2_2:+28575837 Msa0246850:three_prime_UTR
ACTCGTCTTTGCTCTATAGG+AGG 0.556147 2_2:-28575278 None:intergenic
AAAGATGCAGAGGAAAAGAA+GGG 0.557395 2_2:+28575998 Msa0246850:three_prime_UTR
AACGATGAGATTGAGAAGAG+TGG 0.557745 2_2:-28573414 None:intergenic
CCGTGTCGGTATAATTACAA+TGG 0.559193 2_2:-28576115 None:intergenic
TATATATAACTTCTGCCCCT+AGG 0.563152 2_2:-28575854 None:intergenic
AGTTGTGACGGGTATGGGTG+TGG 0.566633 2_2:+28575301 Msa0246850:CDS
TGGAATTACTGAAGAAGTAA+TGG 0.572336 2_2:+28575630 Msa0246850:CDS
CTGTTTCCAGAGGATTGCTG+GGG 0.573853 2_2:+28575498 Msa0246850:intron
ATCTACTTGATGGTGTTAGT+GGG 0.577119 2_2:+28575363 Msa0246850:CDS
CAAAGACGAGTAGTTGTGAC+GGG 0.584893 2_2:+28575290 Msa0246850:CDS
GAGTTAGATAAAACAAAATG+TGG 0.592751 2_2:+28575655 Msa0246850:CDS
TGGGAGAAGAGAGATTAAGG+TGG 0.610292 2_2:-28573459 None:intergenic
GAAGTGTGAAATTACCAGAA+CGG 0.615575 2_2:-28573492 None:intergenic
TGATTGTGCACAATATCCAA+CGG 0.615962 2_2:+28575406 Msa0246850:CDS
TTGATTGGCTCCGGAATGGG+TGG 0.619435 2_2:+28575682 Msa0246850:CDS
TTACCTCTCCTCTCTGCACG+TGG 0.620968 2_2:+28573356 Msa0246850:CDS
CCATTGTAATTATACCGACA+CGG 0.630705 2_2:+28576115 Msa0246850:three_prime_UTR
AATCTGTGTAATTCTTGGAA+CGG 0.636804 2_2:+28576060 Msa0246850:three_prime_UTR
AGTAATTCCAGCATTGACCA+AGG 0.637726 2_2:-28575617 None:intergenic
GCAAAGACGAGTAGTTGTGA+CGG 0.640661 2_2:+28575289 Msa0246850:CDS
TAAACATATCTCAGCCGTGT+CGG 0.644670 2_2:-28576129 None:intergenic
ATATAACTTCTGCCCCTAGG+TGG 0.660807 2_2:-28575851 None:intergenic
TTGAGTGAGAGAAGAGAACT+GGG 0.664150 2_2:-28573203 None:intergenic
AAGCCACGTGCAGAGAGGAG+AGG 0.672286 2_2:-28573359 None:intergenic
GCAACAAGCCACGTGCAGAG+AGG 0.675110 2_2:-28573364 None:intergenic
ACATCTGGATCATGACCAAG+TGG 0.691170 2_2:-28575329 None:intergenic
GTTAGTAATAAGCCACCTAG+GGG 0.723915 2_2:+28575839 Msa0246850:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAATATAATTGCA+AGG + chr2_2:28574152-28574171 Msa0246850:intron 10.0%
!! AATAAAAAATATAATTGCAA+GGG + chr2_2:28574153-28574172 Msa0246850:intron 10.0%
!! ATTTAAATTTATAGTTTGAT+TGG + chr2_2:28574186-28574205 Msa0246850:intron 10.0%
!! ATGAAAATTGAGTAATTAAT+AGG - chr2_2:28573524-28573543 None:intergenic 15.0%
!! TAATATCAAAAAACTACAAA+AGG - chr2_2:28575470-28575489 None:intergenic 15.0%
!!! GATTTTGATTTTGATTTTTG+TGG - chr2_2:28573236-28573255 None:intergenic 20.0%
!! TCTAAACAAAAATACTTACA+AGG + chr2_2:28574121-28574140 Msa0246850:intron 20.0%
!! AAATTTATAGTTTGATTGGT+TGG + chr2_2:28574190-28574209 Msa0246850:intron 20.0%
!!! AAAACGTTTGAAATTTTTGA+AGG + chr2_2:28574518-28574537 Msa0246850:intron 20.0%
!! GATACAAGATATATAATTTC+TGG + chr2_2:28574540-28574559 Msa0246850:intron 20.0%
!! ATGTATTTCACTTTATTGTA+AGG + chr2_2:28575024-28575043 Msa0246850:intron 20.0%
!! AAAATTTCAAGGTAAAGTAA+TGG + chr2_2:28575211-28575230 Msa0246850:intron 20.0%
!!! TTTTATAAATAAAGATGCAG+AGG + chr2_2:28575988-28576007 Msa0246850:three_prime_UTR 20.0%
!!! TTGATTTTGATTTTTGTGGT+TGG - chr2_2:28573232-28573251 None:intergenic 25.0%
!! ATGATGAATTTTCTCATAGT+TGG + chr2_2:28573614-28573633 Msa0246850:intron 25.0%
!! TTCAAATAATTGACTTTCTC+AGG - chr2_2:28573820-28573839 None:intergenic 25.0%
!!! TTTGGTCATGTAAGTTTTTT+TGG + chr2_2:28573958-28573977 Msa0246850:intron 25.0%
!! AGAGTTGCATTTTTCATTTT+GGG + chr2_2:28574494-28574513 Msa0246850:intron 25.0%
! AGGAATGTAAATTTAGTCAT+TGG + chr2_2:28574726-28574745 Msa0246850:intron 25.0%
! GCTTCAAAACATATATCATA+TGG - chr2_2:28574842-28574861 None:intergenic 25.0%
!!! TTTGAAGCATCTTTTTTCTT+AGG + chr2_2:28574854-28574873 Msa0246850:intron 25.0%
!!! CATCTTTTTTCTTAGGTTTA+TGG + chr2_2:28574861-28574880 Msa0246850:intron 25.0%
! GACATAATTTACTTAAACCT+AGG + chr2_2:28574885-28574904 Msa0246850:intron 25.0%
!!! ATATACAATTTTAACAGCCA+GGG - chr2_2:28574975-28574994 None:intergenic 25.0%
! ATCTATTACTTCTTTCTGAA+TGG + chr2_2:28574993-28575012 Msa0246850:intron 25.0%
!!! ACAACTTTGGAGTTAAAATT+TGG - chr2_2:28575098-28575117 None:intergenic 25.0%
!! TTGAAATTTTACAAAACGTG+CGG - chr2_2:28575201-28575220 None:intergenic 25.0%
! TTCTACAATAATCTACTTGA+TGG + chr2_2:28575353-28575372 Msa0246850:CDS 25.0%
! GAGTTAGATAAAACAAAATG+TGG + chr2_2:28575655-28575674 Msa0246850:CDS 25.0%
! TATAAAGTAAACTGCTGAAT+GGG - chr2_2:28575775-28575794 None:intergenic 25.0%
! ATATAAAGTAAACTGCTGAA+TGG - chr2_2:28575776-28575795 None:intergenic 25.0%
!! AGAACTTTTATAACTGCTAT+AGG + chr2_2:28575958-28575977 Msa0246850:three_prime_UTR 25.0%
!! GAACTTTTATAACTGCTATA+GGG + chr2_2:28575959-28575978 Msa0246850:three_prime_UTR 25.0%
! GAATTCAGTTATTTGGATAA+AGG + chr2_2:28576020-28576039 Msa0246850:three_prime_UTR 25.0%
AAAGAAAGAATGAAGGAGAA+AGG - chr2_2:28573289-28573308 None:intergenic 30.0%
AGAGATGAAAGAAAGAATGA+AGG - chr2_2:28573296-28573315 None:intergenic 30.0%
ATGAAGAGAATGAATGAGAA+TGG - chr2_2:28573325-28573344 None:intergenic 30.0%
ATTCTCATTCATTCTCTTCA+TGG + chr2_2:28573324-28573343 Msa0246850:exon 30.0%
! GAATTTTCTCATAGTTGGTT+TGG + chr2_2:28573619-28573638 Msa0246850:intron 30.0%
!!! TTACACGCGTTTTTTCATTT+TGG + chr2_2:28573940-28573959 Msa0246850:intron 30.0%
GGTATCATGGAACATAAATA+TGG + chr2_2:28574211-28574230 Msa0246850:intron 30.0%
TCATGGAACATAAATATGGT+TGG + chr2_2:28574215-28574234 Msa0246850:intron 30.0%
ACAACCATGATATGATTAGA+GGG - chr2_2:28574447-28574466 None:intergenic 30.0%
TACAACCATGATATGATTAG+AGG - chr2_2:28574448-28574467 None:intergenic 30.0%
! GAGAGTTGCATTTTTCATTT+TGG + chr2_2:28574493-28574512 Msa0246850:intron 30.0%
! GAGTTGCATTTTTCATTTTG+GGG + chr2_2:28574495-28574514 Msa0246850:intron 30.0%
!! CTACATATAAAGCATTGACA+CGG + chr2_2:28574569-28574588 Msa0246850:intron 30.0%
ACGTCTTAAATATGAAGTGT+TGG + chr2_2:28574665-28574684 Msa0246850:intron 30.0%
ATAGTAGAGAATGCAATCAA+TGG - chr2_2:28574922-28574941 None:intergenic 30.0%
!! ACTTTAAGATTATGATTCCC+TGG + chr2_2:28574955-28574974 Msa0246850:intron 30.0%
!! GATATACAATTTTAACAGCC+AGG - chr2_2:28574976-28574995 None:intergenic 30.0%
ATTACTTCTTTCTGAATGGT+TGG + chr2_2:28574997-28575016 Msa0246850:intron 30.0%
TGCTTTCTTGAATGTTTAGT+AGG + chr2_2:28575064-28575083 Msa0246850:intron 30.0%
!! ACTTTGGAGTTAAAATTTGG+AGG - chr2_2:28575095-28575114 None:intergenic 30.0%
AGTAAATCATGAGTCAAATC+TGG - chr2_2:28575138-28575157 None:intergenic 30.0%
! GCACGTTTTGTAAAATTTCA+AGG + chr2_2:28575200-28575219 Msa0246850:intron 30.0%
AGATTATTGTAGAAGACATC+TGG - chr2_2:28575347-28575366 None:intergenic 30.0%
GAAGAAATATTCTTTACCGT+TGG - chr2_2:28575425-28575444 None:intergenic 30.0%
CAACGAATATGAATGAACTT+CGG - chr2_2:28575447-28575466 None:intergenic 30.0%
ATCAAATCTTTCTCAACTGA+TGG + chr2_2:28575523-28575542 Msa0246850:CDS 30.0%
TGGAATTACTGAAGAAGTAA+TGG + chr2_2:28575630-28575649 Msa0246850:CDS 30.0%
!!! ACAAAATGTGGTGTTTTGAT+TGG + chr2_2:28575667-28575686 Msa0246850:CDS 30.0%
GCATAATAACATTCGTTTGT+AGG + chr2_2:28575805-28575824 Msa0246850:three_prime_UTR 30.0%
AATAACATTCGTTTGTAGGA+AGG + chr2_2:28575809-28575828 Msa0246850:three_prime_UTR 30.0%
AAGAAGGGAATTCAGTTATT+TGG + chr2_2:28576013-28576032 Msa0246850:three_prime_UTR 30.0%
CAGTTATTTGGATAAAGGAT+TGG + chr2_2:28576025-28576044 Msa0246850:three_prime_UTR 30.0%
! TATTTGGATAAAGGATTGGT+AGG + chr2_2:28576029-28576048 Msa0246850:three_prime_UTR 30.0%
! ATTTGGATAAAGGATTGGTA+GGG + chr2_2:28576030-28576049 Msa0246850:three_prime_UTR 30.0%
AATCTGTGTAATTCTTGGAA+CGG + chr2_2:28576060-28576079 Msa0246850:three_prime_UTR 30.0%
AATGAAGGAGAAAGGTTAAG+AGG - chr2_2:28573281-28573300 None:intergenic 35.0%
AAGTGTGAAATTACCAGAAC+GGG - chr2_2:28573494-28573513 None:intergenic 35.0%
GAAGTGTGAAATTACCAGAA+CGG - chr2_2:28573495-28573514 None:intergenic 35.0%
! AGTTTGATTGGTTGGTATCA+TGG + chr2_2:28574198-28574217 Msa0246850:intron 35.0%
GGAACATAAATATGGTTGGT+TGG + chr2_2:28574219-28574238 Msa0246850:intron 35.0%
TAGTCAGAACTTCAGAGATA+AGG + chr2_2:28574242-28574261 Msa0246850:intron 35.0%
AACCATGATATGATTAGAGG+GGG - chr2_2:28574445-28574464 None:intergenic 35.0%
CAACCATGATATGATTAGAG+GGG - chr2_2:28574446-28574465 None:intergenic 35.0%
! CTTCATATTTAAGACGTGTC+CGG - chr2_2:28574662-28574681 None:intergenic 35.0%
TAAAGTAATGGCTGTAGCTT+TGG + chr2_2:28575223-28575242 Msa0246850:CDS 35.0%
!! AATCTACTTGATGGTGTTAG+TGG + chr2_2:28575362-28575381 Msa0246850:CDS 35.0%
!! ATCTACTTGATGGTGTTAGT+GGG + chr2_2:28575363-28575382 Msa0246850:CDS 35.0%
TGATTGTGCACAATATCCAA+CGG + chr2_2:28575406-28575425 Msa0246850:CDS 35.0%
TAAGTATTCTCTGTTTCCAG+AGG + chr2_2:28575488-28575507 Msa0246850:intron 35.0%
! TAGCACCAAAGTTTTCAAAG+AGG + chr2_2:28575551-28575570 Msa0246850:CDS 35.0%
! ACCAAAGTTTTCAAAGAGGA+TGG + chr2_2:28575555-28575574 Msa0246850:CDS 35.0%
! TCCATCCTCTTTGAAAACTT+TGG - chr2_2:28575559-28575578 None:intergenic 35.0%
TTGTTAGTAATAAGCCACCT+AGG + chr2_2:28575837-28575856 Msa0246850:three_prime_UTR 35.0%
TGTTAGTAATAAGCCACCTA+GGG + chr2_2:28575838-28575857 Msa0246850:three_prime_UTR 35.0%
TATATATAACTTCTGCCCCT+AGG - chr2_2:28575857-28575876 None:intergenic 35.0%
AGATAACAGTTACTGCAGAT+TGG - chr2_2:28575936-28575955 None:intergenic 35.0%
TAAAGATGCAGAGGAAAAGA+AGG + chr2_2:28575997-28576016 Msa0246850:three_prime_UTR 35.0%
AAAGATGCAGAGGAAAAGAA+GGG + chr2_2:28575998-28576017 Msa0246850:three_prime_UTR 35.0%
AGACCAATCTGTGTAATTCT+TGG + chr2_2:28576055-28576074 Msa0246850:three_prime_UTR 35.0%
GTTCCAAGAATTACACAGAT+TGG - chr2_2:28576061-28576080 None:intergenic 35.0%
! CGTGTCGGTATAATTACAAT+GGG - chr2_2:28576117-28576136 None:intergenic 35.0%
CCATTGTAATTATACCGACA+CGG + chr2_2:28576115-28576134 Msa0246850:three_prime_UTR 35.0%
TTGAGTGAGAGAAGAGAACT+GGG - chr2_2:28573206-28573225 None:intergenic 40.0%
TTTGAGTGAGAGAAGAGAAC+TGG - chr2_2:28573207-28573226 None:intergenic 40.0%
ACGATGAGATTGAGAAGAGT+GGG - chr2_2:28573416-28573435 None:intergenic 40.0%
AACGATGAGATTGAGAAGAG+TGG - chr2_2:28573417-28573436 None:intergenic 40.0%
GATTGGGAGAAGAGAGATTA+AGG - chr2_2:28573465-28573484 None:intergenic 40.0%
GACACAATAAGCTAACGTCA+GGG - chr2_2:28574026-28574045 None:intergenic 40.0%
TGACACAATAAGCTAACGTC+AGG - chr2_2:28574027-28574046 None:intergenic 40.0%
! TTGGGCTAGATATAACGTCT+GGG + chr2_2:28574062-28574081 Msa0246850:intron 40.0%
! ACGTCTGGGATTAAAAGCAA+CGG + chr2_2:28574076-28574095 Msa0246850:intron 40.0%
!! AGCAACGGTGTTTAATTTGC+AGG + chr2_2:28574091-28574110 Msa0246850:intron 40.0%
!! GCAACGGTGTTTAATTTGCA+GGG + chr2_2:28574092-28574111 Msa0246850:intron 40.0%
!! GATAAGGACTTGTTAGCTTC+TGG + chr2_2:28574258-28574277 Msa0246850:intron 40.0%
TACCCCCTCTAATCATATCA+TGG + chr2_2:28574440-28574459 Msa0246850:intron 40.0%
CCACATGTTATAGTCATGTC+TGG - chr2_2:28574604-28574623 None:intergenic 40.0%
CCAGACATGACTATAACATG+TGG + chr2_2:28574601-28574620 Msa0246850:intron 40.0%
! ATTTAAGACGTGTCCGGTAT+TGG - chr2_2:28574656-28574675 None:intergenic 40.0%
GTAAGAGACTAGTACATGAG+TGG + chr2_2:28574694-28574713 Msa0246850:intron 40.0%
! TACATGAGTGGTCTTGCTTT+AGG + chr2_2:28574706-28574725 Msa0246850:intron 40.0%
! TGCATGCATGTTTTGTGACT+TGG + chr2_2:28574752-28574771 Msa0246850:intron 40.0%
! TTTTACAAAACGTGCGGCAT+GGG - chr2_2:28575195-28575214 None:intergenic 40.0%
! ATTTTACAAAACGTGCGGCA+TGG - chr2_2:28575196-28575215 None:intergenic 40.0%
ACTACTCGTCTTTGCTCTAT+AGG - chr2_2:28575284-28575303 None:intergenic 40.0%
AGTGGGATAAGCGAGATTAA+TGG + chr2_2:28575380-28575399 Msa0246850:CDS 40.0%
AAAAAAGCCTTGGTCAATGC+TGG + chr2_2:28575610-28575629 Msa0246850:CDS 40.0%
!! AGTAATTCCAGCATTGACCA+AGG - chr2_2:28575620-28575639 None:intergenic 40.0%
GTTAGTAATAAGCCACCTAG+GGG + chr2_2:28575839-28575858 Msa0246850:three_prime_UTR 40.0%
! CCGTGTCGGTATAATTACAA+TGG - chr2_2:28576118-28576137 None:intergenic 40.0%
TAAACATATCTCAGCCGTGT+CGG - chr2_2:28576132-28576151 None:intergenic 40.0%
TGGGAGAAGAGAGATTAAGG+TGG - chr2_2:28573462-28573481 None:intergenic 45.0%
AGGATATTACCATGTCGACG+TGG - chr2_2:28573800-28573819 None:intergenic 45.0%
TGTAGTCACTTGTGTTGCGT+CGG + chr2_2:28573840-28573859 Msa0246850:intron 45.0%
! TCACGTCGCGTGATTTTGTT+GGG + chr2_2:28574044-28574063 Msa0246850:intron 45.0%
! GTTGGGCTAGATATAACGTC+TGG + chr2_2:28574061-28574080 Msa0246850:intron 45.0%
ATGTGACCAACCATCTGTCT+AGG + chr2_2:28574627-28574646 Msa0246850:intron 45.0%
GTATTGGACACCTAGACAGA+TGG - chr2_2:28574640-28574659 None:intergenic 45.0%
TCTGTCTAGGTGTCCAATAC+CGG + chr2_2:28574640-28574659 Msa0246850:intron 45.0%
CAATGGCTAAGAATACGCCT+AGG - chr2_2:28574905-28574924 None:intergenic 45.0%
CTCCAAAGTTGTGCTCATGT+TGG + chr2_2:28575106-28575125 Msa0246850:intron 45.0%
CTCCAACATGAGCACAACTT+TGG - chr2_2:28575111-28575130 None:intergenic 45.0%
CAAAGTTGTGCTCATGTTGG+AGG + chr2_2:28575109-28575128 Msa0246850:intron 45.0%
ACTCGTCTTTGCTCTATAGG+AGG - chr2_2:28575281-28575300 None:intergenic 45.0%
GCAAAGACGAGTAGTTGTGA+CGG + chr2_2:28575289-28575308 Msa0246850:CDS 45.0%
CAAAGACGAGTAGTTGTGAC+GGG + chr2_2:28575290-28575309 Msa0246850:CDS 45.0%
ACATCTGGATCATGACCAAG+TGG - chr2_2:28575332-28575351 None:intergenic 45.0%
TCTGTTTCCAGAGGATTGCT+GGG + chr2_2:28575497-28575516 Msa0246850:intron 45.0%
ATGTTGTATCTGCTGACAGC+TGG + chr2_2:28575586-28575605 Msa0246850:CDS 45.0%
GACAGCTGGAAAAAAAGCCT+TGG + chr2_2:28575600-28575619 Msa0246850:CDS 45.0%
!!! TGTGGTGTTTTGATTGGCTC+CGG + chr2_2:28575673-28575692 Msa0246850:CDS 45.0%
!!! TGTTTTGATTGGCTCCGGAA+TGG + chr2_2:28575678-28575697 Msa0246850:CDS 45.0%
!!! GTTTTGATTGGCTCCGGAAT+GGG + chr2_2:28575679-28575698 Msa0246850:CDS 45.0%
ATATAACTTCTGCCCCTAGG+TGG - chr2_2:28575854-28575873 None:intergenic 45.0%
CCAAGAAATCTGAGAGTCTC+GGG - chr2_2:28576091-28576110 None:intergenic 45.0%
CCCAAGAAATCTGAGAGTCT+CGG - chr2_2:28576092-28576111 None:intergenic 45.0%
CCGAGACTCTCAGATTTCTT+GGG + chr2_2:28576089-28576108 Msa0246850:three_prime_UTR 45.0%
!! TATAAATTTAAATATGATTA+TGG - chr2_2:28574179-28574198 None:intergenic 5.0%
CCAGAACGGGACATTCGATT+GGG - chr2_2:28573481-28573500 None:intergenic 50.0%
ACCAGAACGGGACATTCGAT+TGG - chr2_2:28573482-28573501 None:intergenic 50.0%
! ACGCCTTTGATCAGAGGTGT+CGG + chr2_2:28573872-28573891 Msa0246850:intron 50.0%
! GTCACGTCGCGTGATTTTGT+TGG + chr2_2:28574043-28574062 Msa0246850:intron 50.0%
TGGACACCTAGACAGATGGT+TGG - chr2_2:28574636-28574655 None:intergenic 50.0%
!! ACGAGTAGTTGTGACGGGTA+TGG + chr2_2:28575295-28575314 Msa0246850:CDS 50.0%
!! CGAGTAGTTGTGACGGGTAT+GGG + chr2_2:28575296-28575315 Msa0246850:CDS 50.0%
CTCTGTTTCCAGAGGATTGC+TGG + chr2_2:28575496-28575515 Msa0246850:intron 50.0%
CTGTTTCCAGAGGATTGCTG+GGG + chr2_2:28575498-28575517 Msa0246850:intron 50.0%
TACCTGCATTCCACCCATTC+CGG - chr2_2:28575695-28575714 None:intergenic 50.0%
CCCGAGACTCTCAGATTTCT+TGG + chr2_2:28576088-28576107 Msa0246850:three_prime_UTR 50.0%
TTACCTCTCCTCTCTGCACG+TGG + chr2_2:28573356-28573375 Msa0246850:CDS 55.0%
CCCAATCGAATGTCCCGTTC+TGG + chr2_2:28573478-28573497 Msa0246850:CDS 55.0%
! CCTCTGATCAAAGGCGTGTC+TGG - chr2_2:28573869-28573888 None:intergenic 55.0%
CCAGACACGCCTTTGATCAG+AGG + chr2_2:28573866-28573885 Msa0246850:intron 55.0%
! GCACCGACACCTCTGATCAA+AGG - chr2_2:28573878-28573897 None:intergenic 55.0%
!! AGTTGTGACGGGTATGGGTG+TGG + chr2_2:28575301-28575320 Msa0246850:CDS 55.0%
ATGGGTGTGGTCACACCACT+TGG + chr2_2:28575314-28575333 Msa0246850:CDS 55.0%
TGATCTCCCCAGCAATCCTC+TGG - chr2_2:28575507-28575526 None:intergenic 55.0%
! TTGATTGGCTCCGGAATGGG+TGG + chr2_2:28575682-28575701 Msa0246850:CDS 55.0%
AAGCCACGTGCAGAGAGGAG+AGG - chr2_2:28573362-28573381 None:intergenic 60.0%
GCAACAAGCCACGTGCAGAG+AGG - chr2_2:28573367-28573386 None:intergenic 60.0%
ATCGTTCTCGTCGTCGCAGC+AGG + chr2_2:28573431-28573450 Msa0246850:CDS 60.0%
! CGACGTGTCAGTGTCGAGTC+TGG - chr2_2:28573776-28573795 None:intergenic 60.0%
ACACGTCGACCACGTCGACA+TGG + chr2_2:28573788-28573807 Msa0246850:intron 60.0%
CTCCGGAATGGGTGGAATGC+AGG + chr2_2:28575690-28575709 Msa0246850:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 28573170 28576155 28573170 ID=Msa0246850;Name=Msa0246850
chr2_2 mRNA 28573170 28576155 28573170 ID=Msa0246850-mRNA-1;Parent=Msa0246850;Name=Msa0246850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=173|1|1|1|1|1|3|420|196
chr2_2 exon 28573170 28573499 28573170 ID=Msa0246850-mRNA-1:exon:10240;Parent=Msa0246850-mRNA-1
chr2_2 exon 28575222 28575427 28575222 ID=Msa0246850-mRNA-1:exon:10241;Parent=Msa0246850-mRNA-1
chr2_2 exon 28575508 28576155 28575508 ID=Msa0246850-mRNA-1:exon:10242;Parent=Msa0246850-mRNA-1
chr2_2 five_prime_UTR 28573170 28573342 28573170 ID=Msa0246850-mRNA-1:five_prime_utr;Parent=Msa0246850-mRNA-1
chr2_2 CDS 28573343 28573499 28573343 ID=Msa0246850-mRNA-1:cds;Parent=Msa0246850-mRNA-1
chr2_2 CDS 28575222 28575427 28575222 ID=Msa0246850-mRNA-1:cds;Parent=Msa0246850-mRNA-1
chr2_2 CDS 28575508 28575735 28575508 ID=Msa0246850-mRNA-1:cds;Parent=Msa0246850-mRNA-1
chr2_2 three_prime_UTR 28575736 28576155 28575736 ID=Msa0246850-mRNA-1:three_prime_utr;Parent=Msa0246850-mRNA-1
Gene Sequence

>Msa0246850

ATGGCTTCTTCACTTACCTCTCCTCTCTGCACGTGGCTTGTTGCTGCTTGCATGTCAGTCACGTGTGACTCCCACTCTTCTCAATCTCATCGTTCTCGTCGTCGCAGCAGGAACAACCACCTTAATCTCTCTTCTCCCAATCGAATGTCCCGTTCTGGTAAAGTAATGGCTGTAGCTTTGGATTCTGCTCAAGAAGTTACAACAACGAAAAAACCTCCTATAGAGCAAAGACGAGTAGTTGTGACGGGTATGGGTGTGGTCACACCACTTGGTCATGATCCAGATGTCTTCTACAATAATCTACTTGATGGTGTTAGTGGGATAAGCGAGATTAATGGTTTTGATTGTGCACAATATCCAACGAGGATTGCTGGGGAGATCAAATCTTTCTCAACTGATGGCTTTGTAGCACCAAAGTTTTCAAAGAGGATGGATAAATTTATGTTGTATCTGCTGACAGCTGGAAAAAAAGCCTTGGTCAATGCTGGAATTACTGAAGAAGTAATGGATGAGTTAGATAAAACAAAATGTGGTGTTTTGATTGGCTCCGGAATGGGTGGAATGCAGGTAATCACTCTCTTGAATTTTTAG
Protein sequence

>Msa0246850

MASSLTSPLCTWLVAACMSVTCDSHSSQSHRSRRRSRNNHLNLSSPNRMSRSGKVMAVALDSAQEVTTTKKPPIEQRRVVVTGMGVVTPLGHDPDVFYNNLLDGVSGISEINGFDCAQYPTRIAGEIKSFSTDGFVAPKFSKRMDKFMLYLLTAGKKALVNAGITEEVMDELDKTKCGVLIGSGMGGMQVITLLNF*