AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1435470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435470 MtrunA17_Chr3g0123861 98.511 403 6 0 1 403 36 438 0.0 816
Msa1435470 MtrunA17_Chr2g0333221 48.744 398 188 5 6 393 34 425 8.54e-130 382
Msa1435470 MtrunA17_Chr6g0478651 44.904 363 189 4 41 393 60 421 5.28e-99 305
Msa1435470 MtrunA17_Chr3g0087741 40.845 355 184 8 47 392 89 426 1.08e-71 231
Msa1435470 MtrunA17_Chr4g0010781 38.736 364 208 6 26 387 71 421 8.66e-71 229
Msa1435470 MtrunA17_Chr5g0426951 34.140 372 211 10 44 399 80 433 3.54e-63 210
Msa1435470 MtrunA17_Chr8g0334811 34.615 338 205 9 48 376 15 345 1.04e-52 181
Msa1435470 MtrunA17_Chr2g0322181 31.728 353 222 9 48 390 14 357 7.75e-39 144
Msa1435470 MtrunA17_Chr4g0025401 33.524 349 215 9 48 388 14 353 5.35e-38 142
Msa1435470 MtrunA17_Chr7g0269841 31.412 347 203 12 48 390 27 342 7.53e-35 131
Msa1435470 MtrunA17_Chr3g0138471 28.863 343 213 11 46 384 3 318 1.99e-30 119
Msa1435470 MtrunA17_Chr4g0006391 26.741 359 225 12 46 390 3 337 4.84e-26 107
Msa1435470 MtrunA17_Chr2g0282741 61.039 77 24 2 46 118 3 77 3.26e-21 94.0
Msa1435470 MtrunA17_Chr2g0282761 51.961 102 36 4 47 145 4 95 1.88e-20 91.7
Msa1435470 MtrunA17_Chr4g0040501 53.763 93 32 3 46 129 3 93 3.52e-20 90.9
Msa1435470 MtrunA17_Chr7g0242951 54.667 75 32 1 47 121 27 99 9.70e-19 88.2
Msa1435470 MtrunA17_Chr2g0324921 53.333 75 33 1 46 118 3 77 6.94e-18 84.0
Msa1435470 MtrunA17_Chr7g0253281 57.143 63 27 0 47 109 54 116 1.52e-16 79.7
Msa1435470 MtrunA17_Chr1g0209551 58.571 70 28 1 47 115 16 85 1.74e-15 77.8
Msa1435470 MtrunA17_Chr7g0263971 55.385 65 27 1 47 109 369 433 2.52e-15 77.4
Msa1435470 MtrunA17_Chr2g0295821 37.500 104 60 1 42 140 99 202 6.34e-15 75.5
Msa1435470 MtrunA17_Chr2g0328581 33.696 184 71 7 1 163 9 162 7.11e-15 73.2
Msa1435470 MtrunA17_Chr2g0295831 49.296 71 35 1 39 109 103 172 2.53e-14 72.0
Msa1435470 MtrunA17_Chr3g0091361 51.389 72 33 1 47 116 5 76 4.00e-14 70.9
Msa1435470 MtrunA17_Chr5g0414471 57.576 66 27 1 47 111 25 90 7.13e-14 72.8
Msa1435470 MtrunA17_Chr3g0093111 45.679 81 37 2 47 124 2 78 1.19e-13 71.2
Msa1435470 MtrunA17_Chr7g0271891 48.485 66 29 2 48 108 17 82 1.40e-13 68.2
Msa1435470 MtrunA17_Chr8g0344721 42.857 91 41 2 47 130 10 96 4.12e-13 68.6
Msa1435470 MtrunA17_Chr5g0399201 41.250 80 43 1 47 122 12 91 4.68e-13 70.9
Msa1435470 MtrunA17_Chr6g0460411 42.045 88 38 2 47 128 2 82 7.72e-13 67.4
Msa1435470 MtrunA17_Chr6g0460461 42.045 88 38 2 47 128 2 82 9.26e-13 67.0
Msa1435470 MtrunA17_Chr2g0307061 45.070 71 39 0 42 112 105 175 1.26e-12 68.6
Msa1435470 MtrunA17_Chr1g0159491 40.580 69 40 1 45 112 4 72 1.65e-12 62.8
Msa1435470 MtrunA17_Chr1g0173931 53.030 66 26 2 49 111 40 103 1.82e-12 67.4
Msa1435470 MtrunA17_Chr8g0352451 52.174 69 32 1 48 115 73 141 1.88e-12 68.6
Msa1435470 MtrunA17_Chr5g0393371 45.833 72 38 1 45 115 4 75 2.96e-12 67.8
Msa1435470 MtrunA17_Chr4g0021141 48.485 66 33 1 47 111 22 87 3.86e-12 63.5
Msa1435470 MtrunA17_Chr4g0010801 35.833 120 71 3 178 295 49 164 5.98e-12 64.3
Msa1435470 MtrunA17_Chr1g0206121 48.387 62 32 0 47 108 35 96 1.23e-11 65.1
Msa1435470 MtrunA17_Chr5g0415731 38.889 90 46 2 45 125 3 92 1.37e-11 62.0
Msa1435470 MtrunA17_Chr6g0460381 44.118 68 32 1 47 108 3 70 2.00e-11 62.8
Msa1435470 MtrunA17_Chr6g0476751 40.964 83 48 1 42 123 61 143 2.12e-11 65.9
Msa1435470 MtrunA17_Chr1g0178901 31.690 142 86 2 45 186 65 195 2.39e-11 65.5
Msa1435470 MtrunA17_Chr8g0378161 38.889 72 41 1 41 109 54 125 2.99e-11 62.4
Msa1435470 MtrunA17_Chr5g0415751 25.581 129 94 2 257 384 26 153 3.79e-11 61.2
Msa1435470 MtrunA17_Chr2g0301881 40.741 81 45 2 47 126 99 177 5.06e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435470 AT2G22360.1 74.690 403 96 4 1 399 37 437 0.0 620
Msa1435470 AT4G39960.1 71.220 410 106 4 4 403 40 447 0.0 592
Msa1435470 AT4G39960.2 71.220 410 106 4 4 403 40 447 0.0 592
Msa1435470 AT1G80030.4 51.743 373 162 7 35 393 62 430 9.25e-126 372
Msa1435470 AT1G80030.2 51.743 373 162 7 35 393 62 430 1.45e-125 372
Msa1435470 AT1G80030.3 51.743 373 162 7 35 393 62 430 1.45e-125 372
Msa1435470 AT1G80030.1 51.743 373 162 7 35 393 62 430 1.45e-125 372
Msa1435470 AT3G17830.1 46.076 395 196 6 7 390 29 417 1.30e-106 324
Msa1435470 AT5G48030.1 39.898 391 209 9 25 401 75 453 9.54e-77 245
Msa1435470 AT1G28210.3 33.994 353 211 6 47 392 48 385 4.97e-58 195
Msa1435470 AT1G28210.1 33.994 353 211 6 47 392 48 385 6.12e-58 195
Msa1435470 AT1G28210.2 33.994 353 211 6 47 392 48 385 1.22e-57 194
Msa1435470 AT5G22060.1 32.676 355 212 10 48 388 15 356 3.20e-43 156
Msa1435470 AT3G44110.1 32.782 363 220 11 48 399 15 364 1.36e-40 149
Msa1435470 AT3G44110.2 32.024 331 203 10 48 367 15 334 2.86e-34 130
Msa1435470 AT3G62600.1 29.577 355 217 11 40 390 19 344 1.51e-30 120
Msa1435470 AT5G01390.1 25.706 354 230 9 46 390 3 332 7.38e-30 118
Msa1435470 AT3G08910.1 26.829 369 194 12 46 390 3 319 8.38e-27 109
Msa1435470 AT3G08970.1 56.250 80 33 1 46 125 26 103 1.64e-20 94.0
Msa1435470 AT3G08970.2 56.250 80 33 1 46 125 26 103 1.64e-20 94.0
Msa1435470 AT5G25530.2 48.039 102 44 3 47 139 4 105 4.74e-20 90.9
Msa1435470 AT4G28480.2 54.054 74 32 1 47 118 4 77 2.44e-18 85.1
Msa1435470 AT4G28480.2 26.897 145 102 3 247 390 147 288 1.88e-11 64.7
Msa1435470 AT2G20560.1 50.000 88 40 2 46 129 3 90 2.62e-18 85.9
Msa1435470 AT2G20560.1 25.185 135 99 2 257 390 202 335 4.61e-12 67.0
Msa1435470 AT4G28480.1 53.333 75 33 1 46 118 3 77 4.33e-18 85.5
Msa1435470 AT4G28480.1 25.926 135 98 2 257 390 213 346 9.34e-11 63.2
Msa1435470 AT5G25530.1 44.545 110 44 4 47 139 4 113 1.58e-17 83.6
Msa1435470 AT1G10350.1 49.333 75 36 1 46 118 3 77 5.49e-17 82.0
Msa1435470 AT1G10350.1 31.538 130 84 4 257 384 213 339 6.42e-11 63.9
Msa1435470 AT5G01390.3 52.778 72 32 1 46 115 3 74 6.78e-17 80.1
Msa1435470 AT5G01390.4 53.521 71 31 1 47 115 4 74 9.42e-17 79.3
Msa1435470 AT5G01390.2 52.778 72 32 1 46 115 3 74 1.15e-16 80.1
Msa1435470 AT3G47940.1 52.703 74 32 1 46 116 3 76 2.07e-16 80.5
Msa1435470 AT3G47940.1 24.865 185 131 5 203 384 160 339 5.66e-12 67.0
Msa1435470 AT1G59725.1 50.000 76 35 1 46 118 3 78 3.90e-16 79.3
Msa1435470 AT1G59725.1 27.692 130 90 3 257 384 194 321 4.38e-11 64.3
Msa1435470 AT1G59980.2 59.375 64 25 1 49 111 25 88 2.14e-15 77.4
Msa1435470 AT5G59610.2 46.753 77 41 0 31 107 57 133 2.32e-15 75.9
Msa1435470 AT1G59980.1 59.375 64 25 1 49 111 25 88 3.08e-15 77.4
Msa1435470 AT5G59610.1 46.753 77 41 0 31 107 57 133 5.29e-15 75.1
Msa1435470 AT5G59610.4 46.753 77 41 0 31 107 57 133 5.29e-15 75.1
Msa1435470 AT5G18140.1 52.381 63 30 0 47 109 77 139 5.37e-15 75.9
Msa1435470 AT5G59610.3 46.753 77 41 0 31 107 57 133 5.66e-15 75.1
Msa1435470 AT5G59610.5 46.753 77 41 0 31 107 57 133 5.66e-15 75.1
Msa1435470 AT1G56300.2 48.529 68 30 2 46 108 12 79 8.15e-15 70.9
Msa1435470 AT1G56300.1 50.000 66 28 2 48 108 14 79 1.05e-14 71.6
Msa1435470 AT2G41000.2 44.086 93 41 3 46 132 2 89 2.83e-14 72.0
Msa1435470 AT3G57340.1 38.095 105 56 2 42 137 108 212 4.91e-14 73.6
Msa1435470 AT3G57340.2 38.095 105 56 2 42 137 108 212 4.91e-14 73.6
Msa1435470 AT5G49060.2 49.315 73 36 1 39 111 92 163 5.48e-14 73.2
Msa1435470 AT5G49060.1 49.315 73 36 1 39 111 92 163 5.48e-14 73.2
Msa1435470 AT5G05750.1 35.185 108 62 1 42 141 109 216 7.11e-14 72.0
Msa1435470 AT2G41000.1 44.565 92 40 3 47 132 3 89 7.54e-14 70.1
Msa1435470 AT1G24120.1 56.061 66 28 1 47 111 20 85 9.64e-14 72.8
Msa1435470 AT1G68370.1 54.286 70 31 1 47 115 17 86 1.75e-13 72.0
Msa1435470 AT5G03160.1 50.769 65 30 1 47 109 370 434 1.93e-13 72.0
Msa1435470 AT4G39150.3 35.833 120 69 3 45 161 4 118 2.77e-13 70.9
Msa1435470 AT4G39150.1 35.833 120 69 3 45 161 4 118 2.77e-13 70.9
Msa1435470 AT4G39150.2 35.833 120 69 3 45 161 4 118 2.77e-13 70.9
Msa1435470 AT4G13830.3 42.683 82 42 2 45 123 64 143 1.84e-12 65.9
Msa1435470 AT2G21510.3 43.836 73 40 1 45 116 4 76 1.98e-12 68.6
Msa1435470 AT2G21510.1 43.836 73 40 1 45 116 4 76 1.98e-12 68.6
Msa1435470 AT2G21510.5 43.836 73 40 1 45 116 4 76 2.09e-12 68.2
Msa1435470 AT2G21510.2 43.836 73 40 1 45 116 4 76 2.16e-12 68.2
Msa1435470 AT2G21510.4 43.836 73 40 1 45 116 4 76 2.16e-12 68.2
Msa1435470 AT4G13830.1 42.683 82 42 2 45 123 64 143 2.29e-12 65.1
Msa1435470 AT1G76700.1 48.611 72 36 1 45 115 4 75 4.88e-12 67.4
Msa1435470 AT5G06910.1 38.043 92 51 2 49 139 31 117 4.92e-12 66.6
Msa1435470 AT4G13830.2 42.683 82 42 2 45 123 64 143 5.36e-12 65.1
Msa1435470 AT5G16650.1 50.000 66 32 1 47 111 11 76 8.39e-12 62.8
Msa1435470 AT2G35720.1 43.836 73 37 1 47 115 15 87 1.00e-11 67.0
Msa1435470 AT2G17880.1 40.909 88 46 3 44 128 65 149 1.03e-11 63.2
Msa1435470 AT1G79940.4 43.210 81 43 2 47 126 99 177 1.42e-11 66.6
Msa1435470 AT5G16650.2 50.794 63 30 1 47 108 11 73 1.56e-11 62.8
Msa1435470 AT1G79940.2 43.210 81 43 2 47 126 99 177 1.68e-11 66.6
Msa1435470 AT1G79940.3 43.210 81 43 2 47 126 99 177 1.68e-11 66.6
Msa1435470 AT1G79940.1 43.210 81 43 2 47 126 99 177 1.68e-11 66.6
Msa1435470 AT1G21080.3 38.542 96 49 2 45 139 4 90 2.27e-11 65.5
Msa1435470 AT4G37480.1 46.154 65 32 1 49 110 58 122 2.39e-11 65.9
Msa1435470 AT1G21080.1 38.542 96 49 2 45 139 4 90 2.45e-11 65.5
Msa1435470 AT5G37380.8 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.4 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.7 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.2 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.6 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.1 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.5 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.3 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT5G37380.9 47.059 68 36 0 42 109 61 128 3.21e-11 65.1
Msa1435470 AT2G25560.1 41.758 91 48 1 41 131 60 145 4.79e-11 65.1
Msa1435470 AT4G09350.1 47.619 63 31 1 48 108 107 169 4.95e-11 63.2
Msa1435470 AT1G72070.1 44.262 61 32 1 38 98 30 88 5.38e-11 60.5
Msa1435470 AT1G72070.2 45.000 60 31 1 38 97 30 87 6.37e-11 59.3
Msa1435470 AT4G36040.1 38.053 113 54 4 3 109 29 131 6.58e-11 60.8
Msa1435470 AT3G12170.2 34.188 117 64 4 31 139 24 135 6.86e-11 63.2
Msa1435470 AT3G12170.1 38.043 92 51 3 49 139 13 99 9.44e-11 62.4
Msa1435470 AT1G44160.1 30.075 133 91 2 259 390 221 352 9.50e-11 63.2

Find 143 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATTCTGGTAGCCGTTTA+AGG 0.182163 tig0027706:+2860 Msa1435470:CDS
ACAGCATTGGATTATATTCT+TGG 0.219663 tig0027706:+3494 Msa1435470:three_prime_UTR
CATTCATGACCTTGGTATAT+AGG 0.235099 tig0027706:+3344 Msa1435470:three_prime_UTR
TTTGGGGAGGCAGGGCTTAA+AGG 0.247726 tig0027706:+2304 Msa1435470:CDS
CGGTCCATATATGACAAATT+TGG 0.261820 tig0027706:+2286 Msa1435470:CDS
TCACATCTGGATGATAATTT+CGG 0.265510 tig0027706:-1764 None:intergenic
TACAAGCAGGATAAGCTTTA+TGG 0.275354 tig0027706:+91 Msa1435470:CDS
GGTCCATATATGACAAATTT+GGG 0.277603 tig0027706:+2287 Msa1435470:CDS
CTCCCCAAATTTGTCATATA+TGG 0.277754 tig0027706:-2290 None:intergenic
AATCTTGGGGACTACAATTA+AGG 0.292885 tig0027706:+3023 Msa1435470:CDS
AATGAAGCATCCATAATAAA+AGG 0.301948 tig0027706:-3325 None:intergenic
CTTAAAGGTTCACCGGGATT+TGG 0.302482 tig0027706:+2319 Msa1435470:CDS
GTCTTATATTGATGCAATCT+TGG 0.313538 tig0027706:+3008 Msa1435470:CDS
ACCCCTTGCAGTACATGTTC+TGG 0.319612 tig0027706:+2784 Msa1435470:CDS
GTTCCAGCCGGTGTGGATTC+TGG 0.326305 tig0027706:+2847 Msa1435470:CDS
TTGTAAATGTTTCAGCTTAC+CGG 0.333265 tig0027706:+1733 Msa1435470:intron
TCTCGTTTAAGCACGGGATC+CGG 0.337729 tig0027706:-2955 None:intergenic
CACATCTGGATGATAATTTC+GGG 0.342735 tig0027706:-1763 None:intergenic
TGGTGCAGAAGAGAAATTTA+AGG 0.344525 tig0027706:+2138 Msa1435470:CDS
TCTTATATTGATGCAATCTT+GGG 0.353264 tig0027706:+3009 Msa1435470:CDS
CAAATCCGCCTATGTCTATA+AGG 0.354679 tig0027706:-3375 None:intergenic
TTCCAGAGTTACCTTCATTT+CGG 0.363610 tig0027706:-2885 None:intergenic
CTTCATTTCGGACCCTTAAA+CGG 0.373498 tig0027706:-2873 None:intergenic
ATTAAGGTTCCGACTGTAGA+TGG 0.378346 tig0027706:+3039 Msa1435470:CDS
GGATTCTGGTAGCCGTTTAA+GGG 0.391936 tig0027706:+2861 Msa1435470:CDS
TGTGGTACTTGCGATGGTTC+AGG 0.397485 tig0027706:+2622 Msa1435470:CDS
ATGTAACACATGTGGTGGTC+AGG 0.403113 tig0027706:+2672 Msa1435470:CDS
AGCGACTCAAACAGATCAAA+AGG 0.403699 tig0027706:-2457 None:intergenic
GATAATTTCGGGCTAGCTTC+CGG 0.405239 tig0027706:-1752 None:intergenic
AATACTCACTTGTTCACATC+TGG 0.408035 tig0027706:-1777 None:intergenic
CTCGTTTAAGCACGGGATCC+GGG 0.419118 tig0027706:-2954 None:intergenic
CAGGACTACTACACCGTCCT+TGG 0.424406 tig0027706:+379 Msa1435470:intron
ACCAGGATCAACACTTGTTA+TGG 0.426673 tig0027706:+3095 Msa1435470:CDS
TGCTGAATAAGAAGAATATG+AGG 0.439283 tig0027706:+3136 Msa1435470:CDS
GTGGATTTGAAAATCCCATC+TGG 0.440632 tig0027706:+3066 Msa1435470:CDS
CCCCTTGCAGTACATGTTCT+GGG 0.441535 tig0027706:+2785 Msa1435470:CDS
TATGACAAATTTGGGGAGGC+AGG 0.442220 tig0027706:+2295 Msa1435470:CDS
GTACATGTTCTGGGGATGGC+CGG 0.445163 tig0027706:+2794 Msa1435470:CDS
CATCTGGATGATTTAGTCCC+TGG 0.446833 tig0027706:-2652 None:intergenic
ACTTTATGCTGCAGAGATCC+TGG 0.448540 tig0027706:+2118 Msa1435470:intron
CTGTTTGAGTCGCTATTTGA+GGG 0.452801 tig0027706:+2466 Msa1435470:CDS
AGGACTACTACACCGTCCTT+GGG 0.453339 tig0027706:+380 Msa1435470:intron
GGAAAGAAAGGTGGTTCTCC+TGG 0.465052 tig0027706:+2904 Msa1435470:CDS
TTAAATTTCTCTTCTGCACC+AGG 0.476100 tig0027706:-2136 None:intergenic
ACCACCACATGTGTTACATC+TGG 0.476919 tig0027706:-2668 None:intergenic
TGCTCGTCTTGCAATGGGAC+TGG 0.478835 tig0027706:+2751 Msa1435470:CDS
TTAGTCAATGTTATTCATGA+AGG 0.480715 tig0027706:+3525 Msa1435470:three_prime_UTR
TGACTTGCTCGTCTTGCAAT+GGG 0.482426 tig0027706:+2746 Msa1435470:CDS
GATGATATTTCGTGGGAAGT+TGG 0.483750 tig0027706:+3274 Msa1435470:exon
CGTTTAAGGGTCCGAAATGA+AGG 0.488601 tig0027706:+2874 Msa1435470:CDS
TCTGTTTGAGTCGCTATTTG+AGG 0.489195 tig0027706:+2465 Msa1435470:CDS
ATGACTTGCTCGTCTTGCAA+TGG 0.491631 tig0027706:+2745 Msa1435470:CDS
ATGGTCGCAGCAGACAATCT+TGG 0.494344 tig0027706:+2494 Msa1435470:CDS
TGCATCGTCGAAGGGGATCA+AGG 0.494975 tig0027706:+180 Msa1435470:CDS
CTACCAGAATCCACACCGGC+TGG 0.495088 tig0027706:-2850 None:intergenic
GAAGGTAACTCTGGAAAGAA+AGG 0.495407 tig0027706:+2892 Msa1435470:CDS
AAAGGTTCACCGGGATTTGG+TGG 0.502293 tig0027706:+2322 Msa1435470:CDS
GTTCTGGGGATGGCCGGGTG+AGG 0.503033 tig0027706:+2800 Msa1435470:CDS
GGATGCTTCATTCATGACCT+TGG 0.503641 tig0027706:+3336 Msa1435470:three_prime_UTR
TGTTGTTCTTGAAGTTATCC+CGG 0.507023 tig0027706:+2936 Msa1435470:CDS
TGGAAAAGGAGATAGAGATT+AGG 0.510570 tig0027706:+2587 Msa1435470:CDS
GGTGTCTTCTCGTTTAAGCA+CGG 0.510856 tig0027706:-2962 None:intergenic
TACATGTTCTGGGGATGGCC+GGG 0.512446 tig0027706:+2795 Msa1435470:CDS
AAGGTTCACCGGGATTTGGT+GGG 0.517220 tig0027706:+2323 Msa1435470:CDS
TAGTCAATGTTATTCATGAA+GGG 0.518862 tig0027706:+3526 Msa1435470:three_prime_UTR
TCTTGGAATGGAGCAATGAA+TGG 0.519565 tig0027706:+2511 Msa1435470:CDS
GTGTCTTCTCGTTTAAGCAC+GGG 0.522378 tig0027706:-2961 None:intergenic
GATCCTGGTTGTGTCCCAGA+TGG 0.522621 tig0027706:-3081 None:intergenic
ACTAAATCTGAAATTAAGAC+TGG 0.522708 tig0027706:+418 Msa1435470:CDS
TTTCTTGCAGTGCTACAAGC+AGG 0.523918 tig0027706:+78 None:intergenic
TGCAGTACATGTTCTGGGGA+TGG 0.526866 tig0027706:+2790 Msa1435470:CDS
CACATGTGGTGGTCAGGGAC+GGG 0.527198 tig0027706:+2678 Msa1435470:CDS
GCCATAACAAGTGTTGATCC+TGG 0.527492 tig0027706:-3096 None:intergenic
GGTTCACCGGGATTTGGTGG+GGG 0.527719 tig0027706:+2325 Msa1435470:CDS
AAAACTGATTGAGGAACTCT+CGG 0.528888 tig0027706:+3218 Msa1435470:CDS
TGAGTCGCTATTTGAGGGCA+TGG 0.529744 tig0027706:+2471 Msa1435470:CDS
ATCCTGGTTGTGTCCCAGAT+GGG 0.532879 tig0027706:-3080 None:intergenic
TTGTTACTCCTTATAGACAT+AGG 0.535142 tig0027706:+3367 Msa1435470:three_prime_UTR
ACATAGGCGGATTTGAAGAA+AGG 0.536912 tig0027706:+3383 Msa1435470:three_prime_UTR
ATCCCATCTGGGACACAACC+AGG 0.538117 tig0027706:+3078 Msa1435470:CDS
CACTCAAACAAGGACTCCGT+TGG 0.539049 tig0027706:+2705 Msa1435470:CDS
ACACTTGTTATGGCAAAGAA+AGG 0.539668 tig0027706:+3105 Msa1435470:CDS
TCGCTATTTGAGGGCATGGA+TGG 0.540022 tig0027706:+2475 Msa1435470:CDS
ATGACAAATTTGGGGAGGCA+GGG 0.541510 tig0027706:+2296 Msa1435470:CDS
ATCAAACTTTGCATCGTCGA+AGG 0.545133 tig0027706:+171 Msa1435470:CDS
ACTCAAACAAGGACTCCGTT+GGG 0.546387 tig0027706:+2706 Msa1435470:CDS
CGCAGCAGACAATCTTGGAA+TGG 0.550184 tig0027706:+2499 Msa1435470:CDS
TTTACAAGAATATAAAATGT+TGG 0.551313 tig0027706:-2983 None:intergenic
CGGATTTGAAGAAAGGACAT+AGG 0.552842 tig0027706:+3390 Msa1435470:three_prime_UTR
CGATGAGAGAAAACTGATTG+AGG 0.553553 tig0027706:+3209 Msa1435470:CDS
GTCCGAAATGAAGGTAACTC+TGG 0.554384 tig0027706:+2883 Msa1435470:CDS
GCTGAATAAGAAGAATATGA+GGG 0.556781 tig0027706:+3137 Msa1435470:CDS
TCAAACTTTGCATCGTCGAA+GGG 0.557101 tig0027706:+172 Msa1435470:CDS
GTCAATGTTATTCATGAAGG+GGG 0.557969 tig0027706:+3528 Msa1435470:three_prime_UTR
AGGTTCACCGGGATTTGGTG+GGG 0.558467 tig0027706:+2324 Msa1435470:CDS
ACACATGTGGTGGTCAGGGA+CGG 0.560533 tig0027706:+2677 Msa1435470:CDS
CCCCAGAACATGTACTGCAA+GGG 0.565096 tig0027706:-2786 None:intergenic
GGCAGGGCTTAAAGGTTCAC+CGG 0.569076 tig0027706:+2312 Msa1435470:CDS
CGGTATGAGATGATATTTCG+TGG 0.569871 tig0027706:+3266 Msa1435470:exon
ATATTCTTCTTATTCAGCAG+AGG 0.569920 tig0027706:-3132 None:intergenic
AGCAAAGGCAAGACTGCCGC+CGG 0.579260 tig0027706:+3246 Msa1435470:CDS
GCGACTCAAACAGATCAAAA+GGG 0.581128 tig0027706:-2456 None:intergenic
TGTAACACATGTGGTGGTCA+GGG 0.583052 tig0027706:+2673 Msa1435470:CDS
TGAACCTATATTAAAACGCA+AGG 0.584026 tig0027706:-147 None:intergenic
TCTTGTCAGATGATGAGAAA+CGG 0.584943 tig0027706:+2266 Msa1435470:CDS
TCCCCAGAACATGTACTGCA+AGG 0.589752 tig0027706:-2787 None:intergenic
AGTCAATGTTATTCATGAAG+GGG 0.597617 tig0027706:+3527 Msa1435470:three_prime_UTR
AACAAAGCGGATAAGTCTCA+AGG 0.598548 tig0027706:+2825 Msa1435470:CDS
GCAGGGCTTAAAGGTTCACC+GGG 0.602555 tig0027706:+2313 Msa1435470:CDS
GGTTCCGACTGTAGATGGAA+TGG 0.604592 tig0027706:+3044 Msa1435470:CDS
CACGAAATATCATCTCATAC+CGG 0.605585 tig0027706:-3265 None:intergenic
GATGGTTCAGGAGCTAAACC+AGG 0.608802 tig0027706:+2634 Msa1435470:CDS
AGAACAACAAAGAGGTCACC+AGG 0.612651 tig0027706:-2922 None:intergenic
TGGATTTGAAAATCCCATCT+GGG 0.615278 tig0027706:+3067 Msa1435470:CDS
CCCAGAACATGTACTGCAAG+GGG 0.617559 tig0027706:-2785 None:intergenic
TCTCAAGGTTCCAGCCGGTG+TGG 0.618146 tig0027706:+2840 Msa1435470:CDS
ATAAGTCTCAAGGTTCCAGC+CGG 0.620771 tig0027706:+2835 Msa1435470:CDS
CTTATATTGATGCAATCTTG+GGG 0.623095 tig0027706:+3010 Msa1435470:CDS
GACGGGTTGTCACTCAAACA+AGG 0.625091 tig0027706:+2695 Msa1435470:CDS
GGTAACTCTGGAAAGAAAGG+TGG 0.630205 tig0027706:+2895 Msa1435470:CDS
TTACTCCTTATAGACATAGG+CGG 0.632114 tig0027706:+3370 Msa1435470:three_prime_UTR
GAACTCTCGGAGTTGAGCAA+AGG 0.632985 tig0027706:+3231 Msa1435470:CDS
GAGAAGTGTGGTACTTGCGA+TGG 0.635595 tig0027706:+2616 Msa1435470:CDS
TCCAGATGTAACACATGTGG+TGG 0.637762 tig0027706:+2667 Msa1435470:CDS
TCCACCATTCCATCTACAGT+CGG 0.638944 tig0027706:-3048 None:intergenic
GGAAATCAGTAATGCGTACG+AGG 0.643998 tig0027706:+2159 Msa1435470:CDS
GGACTACTACACCGTCCTTG+GGG 0.645334 tig0027706:+381 Msa1435470:intron
CCCTTGCAGTACATGTTCTG+GGG 0.646072 tig0027706:+2786 Msa1435470:CDS
TAACTTCAAGAACAACAAAG+AGG 0.649565 tig0027706:-2930 None:intergenic
GACTGCTGAAAGATACCCAA+CGG 0.649757 tig0027706:-2721 None:intergenic
CTGAATAAGAAGAATATGAG+GGG 0.650416 tig0027706:+3138 Msa1435470:CDS
CAAACTTTGCATCGTCGAAG+GGG 0.657260 tig0027706:+173 Msa1435470:CDS
CATATATGACAAATTTGGGG+AGG 0.659382 tig0027706:+2291 Msa1435470:CDS
GGTATGAGATGATATTTCGT+GGG 0.660372 tig0027706:+3267 Msa1435470:exon
TCCGACTGTAGATGGAATGG+TGG 0.660929 tig0027706:+3047 Msa1435470:CDS
GTCCATATATGACAAATTTG+GGG 0.662707 tig0027706:+2288 Msa1435470:CDS
TCATCCAGATGTAACACATG+TGG 0.669998 tig0027706:+2664 Msa1435470:CDS
ATTAGGCGACTAGAGAAGTG+TGG 0.694061 tig0027706:+2604 Msa1435470:CDS
GAGTAACAACCTATATACCA+AGG 0.699283 tig0027706:-3353 None:intergenic
ATGGTTCAGGAGCTAAACCA+GGG 0.708190 tig0027706:+2635 Msa1435470:CDS
GCCGGGTGAGGAAAACAAAG+CGG 0.718931 tig0027706:+2812 Msa1435470:CDS
GAAATATCATCTCATACCGG+CGG 0.725473 tig0027706:-3262 None:intergenic
ACGGCTACCAGAATCCACAC+CGG 0.739891 tig0027706:-2854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTCCTTTTATTA+TGG + tig0027706:3315-3334 Msa1435470:three_prime_UTR 10.0%
!! TATTAAATGAGATAATCATT+CGG - tig0027706:1149-1168 None:intergenic 15.0%
!!! AATTTTGAAAGTTTCAAATT+TGG - tig0027706:1400-1419 None:intergenic 15.0%
!!! TTCATTTCACTTTTTTTTTT+TGG + tig0027706:1558-1577 Msa1435470:intron 15.0%
!! TTTACAAGAATATAAAATGT+TGG - tig0027706:2986-3005 None:intergenic 15.0%
!!! TTACATTTTAGTTATATTCT+TGG + tig0027706:3458-3477 Msa1435470:three_prime_UTR 15.0%
!! TACTAATAAAATGTTTGTGA+AGG + tig0027706:614-633 Msa1435470:intron 20.0%
!!! TTAATTCTTGTTCTATGTTT+TGG - tig0027706:694-713 None:intergenic 20.0%
!!! ATTTTGTTTAATGCCAAAAA+AGG + tig0027706:1351-1370 Msa1435470:intron 20.0%
!!! TAAATGTTTAGTTTGAACTT+CGG - tig0027706:1537-1556 None:intergenic 20.0%
!! AATTATGCAACTAGAAATTA+TGG - tig0027706:2035-2054 None:intergenic 20.0%
!!! TTGCATAATTTTTTTTCACA+TGG + tig0027706:2045-2064 Msa1435470:intron 20.0%
!! TAAAGTGAATACACATATAT+AGG - tig0027706:2104-2123 None:intergenic 20.0%
!! TTAAAATTCTCCATAATCTT+TGG - tig0027706:2377-2396 None:intergenic 20.0%
!! TGTAAAATCTTTGTGATTAT+AGG + tig0027706:2423-2442 Msa1435470:intron 20.0%
! TAGATATTCAGCCAATATAT+TGG - tig0027706:344-363 None:intergenic 25.0%
!! ACTAAATCTGAAATTAAGAC+TGG + tig0027706:418-437 Msa1435470:CDS 25.0%
! TCCTAAATTCTTCTGTATTT+AGG + tig0027706:888-907 Msa1435470:intron 25.0%
! ACCTAAATACAGAAGAATTT+AGG - tig0027706:892-911 None:intergenic 25.0%
! TGAAAGTTTCAAATTTGGTT+TGG - tig0027706:1395-1414 None:intergenic 25.0%
!!! ATTCTAGTTTTGGTTTATGT+TGG - tig0027706:1473-1492 None:intergenic 25.0%
!!! TTTTTTTTGGTAATCAATGC+AGG + tig0027706:1571-1590 Msa1435470:intron 25.0%
! TATCAGAAACATCAATTACT+CGG + tig0027706:1620-1639 Msa1435470:intron 25.0%
! TAGAAAAGGAAAAAAAGAGT+GGG - tig0027706:1863-1882 None:intergenic 25.0%
! TATGAACTAACCATGAATTA+GGG - tig0027706:2214-2233 None:intergenic 25.0%
! TTATGAACTAACCATGAATT+AGG - tig0027706:2215-2234 None:intergenic 25.0%
!! ATGTAACTATAATCCTTTTC+AGG + tig0027706:2243-2262 Msa1435470:intron 25.0%
!!! AACTTTAAAGAAGCTGTTTT+CGG + tig0027706:2562-2581 Msa1435470:CDS 25.0%
! TCTTATATTGATGCAATCTT+GGG + tig0027706:3009-3028 Msa1435470:CDS 25.0%
!! AATGAAGCATCCATAATAAA+AGG - tig0027706:3328-3347 None:intergenic 25.0%
! TTAGTCAATGTTATTCATGA+AGG + tig0027706:3525-3544 Msa1435470:three_prime_UTR 25.0%
! TAGTCAATGTTATTCATGAA+GGG + tig0027706:3526-3545 Msa1435470:three_prime_UTR 25.0%
!! GATTCCTTGCGTTTTAATAT+AGG + tig0027706:143-162 Msa1435470:CDS 30.0%
TGAACCTATATTAAAACGCA+AGG - tig0027706:150-169 None:intergenic 30.0%
TCAAGAAGAATACTTTCACA+AGG - tig0027706:297-316 None:intergenic 30.0%
!! GAAAGTATTCTTCTTGAAGT+TGG + tig0027706:300-319 Msa1435470:intron 30.0%
ACTATGACCATCCAATATAT+TGG + tig0027706:330-349 Msa1435470:intron 30.0%
TATTCAGCCAATATATTGGA+TGG - tig0027706:340-359 None:intergenic 30.0%
AAATCATAACAGTCAGAGAA+GGG - tig0027706:458-477 None:intergenic 30.0%
! ATCACAGCTTTGATAACAAA+TGG - tig0027706:782-801 None:intergenic 30.0%
TCCTAATCATTCGTAGTTAA+CGG - tig0027706:845-864 None:intergenic 30.0%
TTGCTTTACAACATACAACA+AGG - tig0027706:1061-1080 None:intergenic 30.0%
ATGAGATAATCATTCGGATT+TGG - tig0027706:1143-1162 None:intergenic 30.0%
CTTAATACCAATACTGAAGA+AGG + tig0027706:1191-1210 Msa1435470:intron 30.0%
TTCAGATAAAATGTCTGTGA+AGG + tig0027706:1214-1233 Msa1435470:intron 30.0%
ACAAAATAAACAGCCGTTTA+GGG - tig0027706:1338-1357 None:intergenic 30.0%
AACAAAATAAACAGCCGTTT+AGG - tig0027706:1339-1358 None:intergenic 30.0%
TTTAATGCCAAAAAAGGACA+TGG + tig0027706:1357-1376 Msa1435470:intron 30.0%
TTAATGCCAAAAAAGGACAT+GGG + tig0027706:1358-1377 Msa1435470:intron 30.0%
ATCAGAAACATCAATTACTC+GGG + tig0027706:1621-1640 Msa1435470:intron 30.0%
TAACAATGCAATGATACCAT+GGG - tig0027706:1703-1722 None:intergenic 30.0%
TTAACAATGCAATGATACCA+TGG - tig0027706:1704-1723 None:intergenic 30.0%
TTGTAAATGTTTCAGCTTAC+CGG + tig0027706:1733-1752 Msa1435470:intron 30.0%
TCACATCTGGATGATAATTT+CGG - tig0027706:1767-1786 None:intergenic 30.0%
AGAAAGCAGATAACTTCAAT+GGG - tig0027706:1818-1837 None:intergenic 30.0%
TAGAAAGCAGATAACTTCAA+TGG - tig0027706:1819-1838 None:intergenic 30.0%
CTAGAAAAGGAAAAAAAGAG+TGG - tig0027706:1864-1883 None:intergenic 30.0%
GCAATAGTGAAATCTAGAAA+AGG - tig0027706:1877-1896 None:intergenic 30.0%
TCTAGATTTCACTATTGCTA+TGG + tig0027706:1879-1898 Msa1435470:intron 30.0%
GGTCCATATATGACAAATTT+GGG + tig0027706:2287-2306 Msa1435470:CDS 30.0%
GTCCATATATGACAAATTTG+GGG + tig0027706:2288-2307 Msa1435470:CDS 30.0%
AAATTCTCCATAATCTTTGG+AGG - tig0027706:2374-2393 None:intergenic 30.0%
TAACTTCAAGAACAACAAAG+AGG - tig0027706:2933-2952 None:intergenic 30.0%
GTCTTATATTGATGCAATCT+TGG + tig0027706:3008-3027 Msa1435470:CDS 30.0%
CTTATATTGATGCAATCTTG+GGG + tig0027706:3010-3029 Msa1435470:CDS 30.0%
ATATTCTTCTTATTCAGCAG+AGG - tig0027706:3135-3154 None:intergenic 30.0%
TGCTGAATAAGAAGAATATG+AGG + tig0027706:3136-3155 Msa1435470:CDS 30.0%
GCTGAATAAGAAGAATATGA+GGG + tig0027706:3137-3156 Msa1435470:CDS 30.0%
CTGAATAAGAAGAATATGAG+GGG + tig0027706:3138-3157 Msa1435470:CDS 30.0%
TTGTTACTCCTTATAGACAT+AGG + tig0027706:3367-3386 Msa1435470:three_prime_UTR 30.0%
!! TCATCTATTGCTTGTTTTCT+TGG + tig0027706:3432-3451 Msa1435470:three_prime_UTR 30.0%
!!! ATGTTGTTTTTTGACAGCAT+TGG + tig0027706:3481-3500 Msa1435470:three_prime_UTR 30.0%
! ACAGCATTGGATTATATTCT+TGG + tig0027706:3494-3513 Msa1435470:three_prime_UTR 30.0%
AGTCAATGTTATTCATGAAG+GGG + tig0027706:3527-3546 Msa1435470:three_prime_UTR 30.0%
TACAAGCAGGATAAGCTTTA+TGG + tig0027706:91-110 Msa1435470:CDS 35.0%
AGCAAAAAAGGTTGAACTTG+GGG - tig0027706:120-139 None:intergenic 35.0%
GAGCAAAAAAGGTTGAACTT+GGG - tig0027706:121-140 None:intergenic 35.0%
!!! CAAGTTCAACCTTTTTTGCT+CGG + tig0027706:120-139 Msa1435470:CDS 35.0%
!!! AAGTTCAACCTTTTTTGCTC+GGG + tig0027706:121-140 Msa1435470:CDS 35.0%
!!! TAGAATTTTTTGACACCCCA+AGG - tig0027706:399-418 None:intergenic 35.0%
CAAATCATAACAGTCAGAGA+AGG - tig0027706:459-478 None:intergenic 35.0%
TCCGTTAACTACGAATGATT+AGG + tig0027706:841-860 Msa1435470:intron 35.0%
AACTACGAATGATTAGGATC+AGG + tig0027706:847-866 Msa1435470:intron 35.0%
ACTACGAATGATTAGGATCA+GGG + tig0027706:848-867 Msa1435470:intron 35.0%
! TGTAATCTTTTGCCTAGATG+CGG + tig0027706:1087-1106 Msa1435470:intron 35.0%
ATGCAAAGATTTGTAACTGC+AGG + tig0027706:1113-1132 Msa1435470:intron 35.0%
TGCAAAGATTTGTAACTGCA+GGG + tig0027706:1114-1133 Msa1435470:intron 35.0%
ATCTGAACCTTCTTCAGTAT+TGG - tig0027706:1201-1220 None:intergenic 35.0%
TAGCAACAAGCATTATCAAG+TGG - tig0027706:1291-1310 None:intergenic 35.0%
CAAAATAAACAGCCGTTTAG+GGG - tig0027706:1337-1356 None:intergenic 35.0%
TAATGCCAAAAAAGGACATG+GGG + tig0027706:1359-1378 Msa1435470:intron 35.0%
TAATCGTCTTGATTGCTCTT+TGG + tig0027706:1419-1438 Msa1435470:intron 35.0%
!!! ACGTACAAGGATTCTAGTTT+TGG - tig0027706:1483-1502 None:intergenic 35.0%
AAAACTAGAATCCTTGTACG+TGG + tig0027706:1482-1501 Msa1435470:intron 35.0%
! TTTGGTAATCAATGCAGGAA+AGG + tig0027706:1576-1595 Msa1435470:intron 35.0%
TAATCAATGCAGGAAAGGTT+TGG + tig0027706:1581-1600 Msa1435470:intron 35.0%
GATGCTATGTGTGATTTACT+TGG + tig0027706:1664-1683 Msa1435470:intron 35.0%
AACAATGCAATGATACCATG+GGG - tig0027706:1702-1721 None:intergenic 35.0%
CACATCTGGATGATAATTTC+GGG - tig0027706:1766-1785 None:intergenic 35.0%
AATACTCACTTGTTCACATC+TGG - tig0027706:1780-1799 None:intergenic 35.0%
CACTATTGCTATGGACAAAA+TGG + tig0027706:1888-1907 Msa1435470:intron 35.0%
! TTGGTATAATCTAAGACACC+CGG - tig0027706:1915-1934 None:intergenic 35.0%
ACTAGAAATTATGGCATGAC+AGG - tig0027706:2026-2045 None:intergenic 35.0%
TTAAATTTCTCTTCTGCACC+AGG - tig0027706:2139-2158 None:intergenic 35.0%
TGGTGCAGAAGAGAAATTTA+AGG + tig0027706:2138-2157 Msa1435470:CDS 35.0%
TCTTGTCAGATGATGAGAAA+CGG + tig0027706:2266-2285 Msa1435470:CDS 35.0%
CGGTCCATATATGACAAATT+TGG + tig0027706:2286-2305 Msa1435470:CDS 35.0%
CTCCCCAAATTTGTCATATA+TGG - tig0027706:2293-2312 None:intergenic 35.0%
CATATATGACAAATTTGGGG+AGG + tig0027706:2291-2310 Msa1435470:CDS 35.0%
! CATAATCTTTGGAGGTAACT+TGG - tig0027706:2366-2385 None:intergenic 35.0%
CAAGTTACCTCCAAAGATTA+TGG + tig0027706:2364-2383 Msa1435470:intron 35.0%
TGGAAAAGGAGATAGAGATT+AGG + tig0027706:2587-2606 Msa1435470:CDS 35.0%
TTCCAGAGTTACCTTCATTT+CGG - tig0027706:2888-2907 None:intergenic 35.0%
TGTTGTTCTTGAAGTTATCC+CGG + tig0027706:2936-2955 Msa1435470:CDS 35.0%
AATCTTGGGGACTACAATTA+AGG + tig0027706:3023-3042 Msa1435470:CDS 35.0%
TGGATTTGAAAATCCCATCT+GGG + tig0027706:3067-3086 Msa1435470:CDS 35.0%
!! ACACTTGTTATGGCAAAGAA+AGG + tig0027706:3105-3124 Msa1435470:CDS 35.0%
AAAACTGATTGAGGAACTCT+CGG + tig0027706:3218-3237 Msa1435470:CDS 35.0%
CACGAAATATCATCTCATAC+CGG - tig0027706:3268-3287 None:intergenic 35.0%
GGTATGAGATGATATTTCGT+GGG + tig0027706:3267-3286 Msa1435470:exon 35.0%
CATTCATGACCTTGGTATAT+AGG + tig0027706:3344-3363 Msa1435470:three_prime_UTR 35.0%
GAGTAACAACCTATATACCA+AGG - tig0027706:3356-3375 None:intergenic 35.0%
TTACTCCTTATAGACATAGG+CGG + tig0027706:3370-3389 Msa1435470:three_prime_UTR 35.0%
GTCAATGTTATTCATGAAGG+GGG + tig0027706:3528-3547 Msa1435470:three_prime_UTR 35.0%
CGAGCAAAAAAGGTTGAACT+TGG - tig0027706:122-141 None:intergenic 40.0%
ATCAAACTTTGCATCGTCGA+AGG + tig0027706:171-190 Msa1435470:CDS 40.0%
TCAAACTTTGCATCGTCGAA+GGG + tig0027706:172-191 Msa1435470:CDS 40.0%
!!! ATTTTTTGACACCCCAAGGA+CGG - tig0027706:395-414 None:intergenic 40.0%
!! GCATCAACTAATGATGATGG+AGG - tig0027706:482-501 None:intergenic 40.0%
GACGCATCAACTAATGATGA+TGG - tig0027706:485-504 None:intergenic 40.0%
TCTTGACAGAGTTAGCTGTT+AGG - tig0027706:665-684 None:intergenic 40.0%
!! TTAACGGACTAAGGCTAATG+TGG - tig0027706:829-848 None:intergenic 40.0%
!! CATTCGTAGTTAACGGACTA+AGG - tig0027706:838-857 None:intergenic 40.0%
! GTAATCTTTTGCCTAGATGC+GGG + tig0027706:1088-1107 Msa1435470:intron 40.0%
AATGCCAAAAAAGGACATGG+GGG + tig0027706:1360-1379 Msa1435470:intron 40.0%
CTCTTTGGTATTGTATTGCG+AGG + tig0027706:1434-1453 Msa1435470:intron 40.0%
! TTGAGAGGAAGTTTTCCAAG+AGG - tig0027706:1509-1528 None:intergenic 40.0%
ACAATGCAATGATACCATGG+GGG - tig0027706:1701-1720 None:intergenic 40.0%
GATAACTTCAATGGGAAGTG+AGG - tig0027706:1810-1829 None:intergenic 40.0%
TTGCTATGGACAAAATGGTC+CGG + tig0027706:1893-1912 Msa1435470:intron 40.0%
AAATGACATGCTGCAAGCAT+TGG - tig0027706:1934-1953 None:intergenic 40.0%
CTTCATCTAACAGGCAATTC+AGG - tig0027706:1980-1999 None:intergenic 40.0%
GCTAAACGACTTCATCTAAC+AGG - tig0027706:1989-2008 None:intergenic 40.0%
ACACAACTGACCCTAATTCA+TGG + tig0027706:2201-2220 Msa1435470:intron 40.0%
TCATCTGACAAGACCTGAAA+AGG - tig0027706:2259-2278 None:intergenic 40.0%
GCGACTCAAACAGATCAAAA+GGG - tig0027706:2459-2478 None:intergenic 40.0%
AGCGACTCAAACAGATCAAA+AGG - tig0027706:2460-2479 None:intergenic 40.0%
TCTGTTTGAGTCGCTATTTG+AGG + tig0027706:2465-2484 Msa1435470:CDS 40.0%
CTGTTTGAGTCGCTATTTGA+GGG + tig0027706:2466-2485 Msa1435470:CDS 40.0%
TCTTGGAATGGAGCAATGAA+TGG + tig0027706:2511-2530 Msa1435470:CDS 40.0%
!! TAAAGAAGCTGTTTTCGGAG+TGG + tig0027706:2567-2586 Msa1435470:CDS 40.0%
TCATCCAGATGTAACACATG+TGG + tig0027706:2664-2683 Msa1435470:CDS 40.0%
AACAAAGCGGATAAGTCTCA+AGG + tig0027706:2825-2844 Msa1435470:CDS 40.0%
CTTCATTTCGGACCCTTAAA+CGG - tig0027706:2876-2895 None:intergenic 40.0%
! GAAGGTAACTCTGGAAAGAA+AGG + tig0027706:2892-2911 Msa1435470:CDS 40.0%
ATTAAGGTTCCGACTGTAGA+TGG + tig0027706:3039-3058 Msa1435470:CDS 40.0%
GTGGATTTGAAAATCCCATC+TGG + tig0027706:3066-3085 Msa1435470:CDS 40.0%
! ACCAGGATCAACACTTGTTA+TGG + tig0027706:3095-3114 Msa1435470:CDS 40.0%
!!! TCATCGCTGCTCAGTTTTTT+AGG - tig0027706:3195-3214 None:intergenic 40.0%
CGATGAGAGAAAACTGATTG+AGG + tig0027706:3209-3228 Msa1435470:CDS 40.0%
GAAATATCATCTCATACCGG+CGG - tig0027706:3265-3284 None:intergenic 40.0%
CGGTATGAGATGATATTTCG+TGG + tig0027706:3266-3285 Msa1435470:exon 40.0%
GATGATATTTCGTGGGAAGT+TGG + tig0027706:3274-3293 Msa1435470:exon 40.0%
CAAATCCGCCTATGTCTATA+AGG - tig0027706:3378-3397 None:intergenic 40.0%
! ACATAGGCGGATTTGAAGAA+AGG + tig0027706:3383-3402 Msa1435470:three_prime_UTR 40.0%
CGGATTTGAAGAAAGGACAT+AGG + tig0027706:3390-3409 Msa1435470:three_prime_UTR 40.0%
CAAGGAATCCCGAGCAAAAA+AGG - tig0027706:132-151 None:intergenic 45.0%
CAAACTTTGCATCGTCGAAG+GGG + tig0027706:173-192 Msa1435470:CDS 45.0%
TCTAATATGTACCCGCATGC+AGG + tig0027706:360-379 Msa1435470:intron 45.0%
CAAGTGGCAGACTTCATTGA+TGG - tig0027706:1275-1294 None:intergenic 45.0%
TAAACAGCCGTTTAGGGGTT+GGG - tig0027706:1332-1351 None:intergenic 45.0%
ATAAACAGCCGTTTAGGGGT+TGG - tig0027706:1333-1352 None:intergenic 45.0%
ATGCCAAAAAAGGACATGGG+GGG + tig0027706:1361-1380 Msa1435470:intron 45.0%
! TGGTTATGCATTTTCCCCCA+TGG + tig0027706:1684-1703 Msa1435470:intron 45.0%
!! GATAATTTCGGGCTAGCTTC+CGG - tig0027706:1755-1774 None:intergenic 45.0%
AGTGGGAACAGAGCAAAAGA+CGG - tig0027706:1846-1865 None:intergenic 45.0%
TGCTATGGACAAAATGGTCC+GGG + tig0027706:1894-1913 Msa1435470:intron 45.0%
ACTTTATGCTGCAGAGATCC+TGG + tig0027706:2118-2137 Msa1435470:intron 45.0%
GGAAATCAGTAATGCGTACG+AGG + tig0027706:2159-2178 Msa1435470:CDS 45.0%
TGCGTACGAGGTAAAACTAG+AGG + tig0027706:2171-2190 Msa1435470:intron 45.0%
TATGACAAATTTGGGGAGGC+AGG + tig0027706:2295-2314 Msa1435470:CDS 45.0%
ATGACAAATTTGGGGAGGCA+GGG + tig0027706:2296-2315 Msa1435470:CDS 45.0%
CTTAAAGGTTCACCGGGATT+TGG + tig0027706:2319-2338 Msa1435470:CDS 45.0%
!! AGCTGTTTTCGGAGTGGAAA+AGG + tig0027706:2573-2592 Msa1435470:CDS 45.0%
ATTAGGCGACTAGAGAAGTG+TGG + tig0027706:2604-2623 Msa1435470:CDS 45.0%
ATGGTTCAGGAGCTAAACCA+GGG + tig0027706:2635-2654 Msa1435470:CDS 45.0%
CATCTGGATGATTTAGTCCC+TGG - tig0027706:2655-2674 None:intergenic 45.0%
TCCAGATGTAACACATGTGG+TGG + tig0027706:2667-2686 Msa1435470:CDS 45.0%
ACCACCACATGTGTTACATC+TGG - tig0027706:2671-2690 None:intergenic 45.0%
ATGTAACACATGTGGTGGTC+AGG + tig0027706:2672-2691 Msa1435470:CDS 45.0%
TGTAACACATGTGGTGGTCA+GGG + tig0027706:2673-2692 Msa1435470:CDS 45.0%
ACTCAAACAAGGACTCCGTT+GGG + tig0027706:2706-2725 Msa1435470:CDS 45.0%
GACTGCTGAAAGATACCCAA+CGG - tig0027706:2724-2743 None:intergenic 45.0%
!! ATGACTTGCTCGTCTTGCAA+TGG + tig0027706:2745-2764 Msa1435470:CDS 45.0%
!! TGACTTGCTCGTCTTGCAAT+GGG + tig0027706:2746-2765 Msa1435470:CDS 45.0%
ATAAGTCTCAAGGTTCCAGC+CGG + tig0027706:2835-2854 Msa1435470:CDS 45.0%
TGGATTCTGGTAGCCGTTTA+AGG + tig0027706:2860-2879 Msa1435470:CDS 45.0%
GGATTCTGGTAGCCGTTTAA+GGG + tig0027706:2861-2880 Msa1435470:CDS 45.0%
CGTTTAAGGGTCCGAAATGA+AGG + tig0027706:2874-2893 Msa1435470:CDS 45.0%
GTCCGAAATGAAGGTAACTC+TGG + tig0027706:2883-2902 Msa1435470:CDS 45.0%
! GGTAACTCTGGAAAGAAAGG+TGG + tig0027706:2895-2914 Msa1435470:CDS 45.0%
AGAACAACAAAGAGGTCACC+AGG - tig0027706:2925-2944 None:intergenic 45.0%
GTGTCTTCTCGTTTAAGCAC+GGG - tig0027706:2964-2983 None:intergenic 45.0%
GGTGTCTTCTCGTTTAAGCA+CGG - tig0027706:2965-2984 None:intergenic 45.0%
TCCACCATTCCATCTACAGT+CGG - tig0027706:3051-3070 None:intergenic 45.0%
!! GCCATAACAAGTGTTGATCC+TGG - tig0027706:3099-3118 None:intergenic 45.0%
GGATGCTTCATTCATGACCT+TGG + tig0027706:3336-3355 Msa1435470:three_prime_UTR 45.0%
!! TTGTAAAAAAAAATATAAAA+AGG - tig0027706:269-288 None:intergenic 5.0%
!!! TTTTTATATTTTTTTTTACA+AGG + tig0027706:268-287 Msa1435470:intron 5.0%
!!! TTTTATATTTTTTTTTACAA+GGG + tig0027706:269-288 Msa1435470:intron 5.0%
AGGACTACTACACCGTCCTT+GGG + tig0027706:380-399 Msa1435470:intron 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + tig0027706:924-943 Msa1435470:intron 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + tig0027706:923-942 Msa1435470:intron 50.0%
AGAGAGAGAGAGAGAGAGAG+GGG + tig0027706:924-943 Msa1435470:intron 50.0%
TCTTTGCATCTCCCGCATCT+AGG - tig0027706:1102-1121 None:intergenic 50.0%
AAACAGCCGTTTAGGGGTTG+GGG - tig0027706:1331-1350 None:intergenic 50.0%
!!! TAGCCCCCCATGTCCTTTTT+TGG - tig0027706:1367-1386 None:intergenic 50.0%
ATCCTTGTACGTGGTCCTCT+TGG + tig0027706:1491-1510 Msa1435470:intron 50.0%
TTCCAAGAGGACCACGTACA+AGG - tig0027706:1496-1515 None:intergenic 50.0%
TGAACTTCGGCAGTCTTGAG+AGG - tig0027706:1524-1543 None:intergenic 50.0%
AAAGGTTCACCGGGATTTGG+TGG + tig0027706:2322-2341 Msa1435470:CDS 50.0%
! AAGGTTCACCGGGATTTGGT+GGG + tig0027706:2323-2342 Msa1435470:CDS 50.0%
TGAGTCGCTATTTGAGGGCA+TGG + tig0027706:2471-2490 Msa1435470:CDS 50.0%
! TCGCTATTTGAGGGCATGGA+TGG + tig0027706:2475-2494 Msa1435470:CDS 50.0%
ATGGTCGCAGCAGACAATCT+TGG + tig0027706:2494-2513 Msa1435470:CDS 50.0%
CGCAGCAGACAATCTTGGAA+TGG + tig0027706:2499-2518 Msa1435470:CDS 50.0%
! GAGAAGTGTGGTACTTGCGA+TGG + tig0027706:2616-2635 Msa1435470:CDS 50.0%
TGTGGTACTTGCGATGGTTC+AGG + tig0027706:2622-2641 Msa1435470:CDS 50.0%
GATGGTTCAGGAGCTAAACC+AGG + tig0027706:2634-2653 Msa1435470:CDS 50.0%
!! GACGGGTTGTCACTCAAACA+AGG + tig0027706:2695-2714 Msa1435470:CDS 50.0%
CACTCAAACAAGGACTCCGT+TGG + tig0027706:2705-2724 Msa1435470:CDS 50.0%
ACCCCTTGCAGTACATGTTC+TGG + tig0027706:2784-2803 Msa1435470:CDS 50.0%
CCCAGAACATGTACTGCAAG+GGG - tig0027706:2788-2807 None:intergenic 50.0%
CCCCTTGCAGTACATGTTCT+GGG + tig0027706:2785-2804 Msa1435470:CDS 50.0%
CCCCAGAACATGTACTGCAA+GGG - tig0027706:2789-2808 None:intergenic 50.0%
CCCTTGCAGTACATGTTCTG+GGG + tig0027706:2786-2805 Msa1435470:CDS 50.0%
TCCCCAGAACATGTACTGCA+AGG - tig0027706:2790-2809 None:intergenic 50.0%
!! TGCAGTACATGTTCTGGGGA+TGG + tig0027706:2790-2809 Msa1435470:CDS 50.0%
!!! TCCGCTTTGTTTTCCTCACC+CGG - tig0027706:2816-2835 None:intergenic 50.0%
GGAAAGAAAGGTGGTTCTCC+TGG + tig0027706:2904-2923 Msa1435470:CDS 50.0%
TCTCGTTTAAGCACGGGATC+CGG - tig0027706:2958-2977 None:intergenic 50.0%
GGTTCCGACTGTAGATGGAA+TGG + tig0027706:3044-3063 Msa1435470:CDS 50.0%
TCCGACTGTAGATGGAATGG+TGG + tig0027706:3047-3066 Msa1435470:CDS 50.0%
ATCCTGGTTGTGTCCCAGAT+GGG - tig0027706:3083-3102 None:intergenic 50.0%
GAACTCTCGGAGTTGAGCAA+AGG + tig0027706:3231-3250 Msa1435470:CDS 50.0%
TGCATCGTCGAAGGGGATCA+AGG + tig0027706:180-199 Msa1435470:CDS 55.0%
GGTGTAGTAGTCCTGCATGC+GGG - tig0027706:374-393 None:intergenic 55.0%
CGGTGTAGTAGTCCTGCATG+CGG - tig0027706:375-394 None:intergenic 55.0%
CAGGACTACTACACCGTCCT+TGG + tig0027706:379-398 Msa1435470:intron 55.0%
GGACTACTACACCGTCCTTG+GGG + tig0027706:381-400 Msa1435470:intron 55.0%
GAGAGAGAGAGAGAGAGAGG+GGG + tig0027706:973-992 Msa1435470:intron 55.0%
AACAGCCGTTTAGGGGTTGG+GGG - tig0027706:1330-1349 None:intergenic 55.0%
TTTGGGGAGGCAGGGCTTAA+AGG + tig0027706:2304-2323 Msa1435470:CDS 55.0%
GGCAGGGCTTAAAGGTTCAC+CGG + tig0027706:2312-2331 Msa1435470:CDS 55.0%
GCAGGGCTTAAAGGTTCACC+GGG + tig0027706:2313-2332 Msa1435470:CDS 55.0%
!! AGGTTCACCGGGATTTGGTG+GGG + tig0027706:2324-2343 Msa1435470:CDS 55.0%
ACACATGTGGTGGTCAGGGA+CGG + tig0027706:2677-2696 Msa1435470:CDS 55.0%
TGCTCGTCTTGCAATGGGAC+TGG + tig0027706:2751-2770 Msa1435470:CDS 55.0%
!! GTACATGTTCTGGGGATGGC+CGG + tig0027706:2794-2813 Msa1435470:CDS 55.0%
!! TACATGTTCTGGGGATGGCC+GGG + tig0027706:2795-2814 Msa1435470:CDS 55.0%
GCCGGGTGAGGAAAACAAAG+CGG + tig0027706:2812-2831 Msa1435470:CDS 55.0%
ACGGCTACCAGAATCCACAC+CGG - tig0027706:2857-2876 None:intergenic 55.0%
CTCGTTTAAGCACGGGATCC+GGG - tig0027706:2957-2976 None:intergenic 55.0%
! ATCCCATCTGGGACACAACC+AGG + tig0027706:3078-3097 Msa1435470:CDS 55.0%
GATCCTGGTTGTGTCCCAGA+TGG - tig0027706:3084-3103 None:intergenic 55.0%
AGAGAGAGAGAGGGGGAGAG+AGG + tig0027706:980-999 Msa1435470:intron 60.0%
GAGAGAGAGAGGGGGAGAGA+GGG + tig0027706:981-1000 Msa1435470:intron 60.0%
ACCCTCCCCCAACCCCTAAA+CGG + tig0027706:1322-1341 Msa1435470:intron 60.0%
!! GGTTCACCGGGATTTGGTGG+GGG + tig0027706:2325-2344 Msa1435470:CDS 60.0%
CACATGTGGTGGTCAGGGAC+GGG + tig0027706:2678-2697 Msa1435470:CDS 60.0%
TCTCAAGGTTCCAGCCGGTG+TGG + tig0027706:2840-2859 Msa1435470:CDS 60.0%
GTTCCAGCCGGTGTGGATTC+TGG + tig0027706:2847-2866 Msa1435470:CDS 60.0%
CTACCAGAATCCACACCGGC+TGG - tig0027706:2853-2872 None:intergenic 60.0%
AGCAAAGGCAAGACTGCCGC+CGG + tig0027706:3246-3265 Msa1435470:CDS 60.0%
AGAGAGGGGGAGAGAGGGAG+AGG + tig0027706:986-1005 Msa1435470:intron 65.0%
GAGAGGGGGAGAGAGGGAGA+GGG + tig0027706:987-1006 Msa1435470:intron 65.0%
GGGAGAGAGGGAGAGGGAGA+GGG + tig0027706:993-1012 Msa1435470:intron 65.0%
AGAGGGAGAGGGAGAGGGAG+AGG + tig0027706:998-1017 Msa1435470:intron 65.0%
GAGGGAGAGGGAGAGGGAGA+GGG + tig0027706:999-1018 Msa1435470:intron 65.0%
AGAGGGAGAGGGAGAGGGAG+AGG + tig0027706:998-1017 Msa1435470:intron 65.0%
GAGGGAGAGGGAGAGGGAGA+GGG + tig0027706:999-1018 Msa1435470:intron 65.0%
GCCGTTTAGGGGTTGGGGGA+GGG - tig0027706:1326-1345 None:intergenic 65.0%
AGCCGTTTAGGGGTTGGGGG+AGG - tig0027706:1327-1346 None:intergenic 65.0%
!! ACCGGGATTTGGTGGGGGCA+TGG + tig0027706:2330-2349 Msa1435470:CDS 65.0%
CCCATGCCCCCACCAAATCC+CGG - tig0027706:2334-2353 None:intergenic 65.0%
!! CCGGGATTTGGTGGGGGCAT+GGG + tig0027706:2331-2350 Msa1435470:CDS 65.0%
!! CGGGATTTGGTGGGGGCATG+GGG + tig0027706:2332-2351 Msa1435470:CDS 65.0%
!! GGGATTTGGTGGGGGCATGG+GGG + tig0027706:2333-2352 Msa1435470:CDS 65.0%
GGGGAGAGAGGGAGAGGGAG+AGG + tig0027706:992-1011 Msa1435470:intron 70.0%
!! GTTCTGGGGATGGCCGGGTG+AGG + tig0027706:2800-2819 Msa1435470:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0027706 gene 86 3574 86 ID=Msa1435470;Name=Msa1435470
tig0027706 mRNA 86 3574 86 ID=Msa1435470-mRNA-1;Parent=Msa1435470;Name=Msa1435470-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0.83|1|1|1|6|296|403
tig0027706 exon 86 223 86 ID=Msa1435470-mRNA-1:exon:9718;Parent=Msa1435470-mRNA-1
tig0027706 exon 382 439 382 ID=Msa1435470-mRNA-1:exon:9719;Parent=Msa1435470-mRNA-1
tig0027706 exon 1748 1790 1748 ID=Msa1435470-mRNA-1:exon:9720;Parent=Msa1435470-mRNA-1
tig0027706 exon 2132 2180 2132 ID=Msa1435470-mRNA-1:exon:9721;Parent=Msa1435470-mRNA-1
tig0027706 exon 2265 2354 2265 ID=Msa1435470-mRNA-1:exon:9722;Parent=Msa1435470-mRNA-1
tig0027706 exon 2445 3574 2445 ID=Msa1435470-mRNA-1:exon:9723;Parent=Msa1435470-mRNA-1
tig0027706 CDS 86 223 86 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 CDS 382 439 382 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 CDS 1748 1790 1748 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 CDS 2132 2180 2132 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 CDS 2265 2354 2265 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 CDS 2445 3278 2445 ID=Msa1435470-mRNA-1:cds;Parent=Msa1435470-mRNA-1
tig0027706 three_prime_UTR 3279 3574 3279 ID=Msa1435470-mRNA-1:three_prime_utr;Parent=Msa1435470-mRNA-1
Gene Sequence

>Msa1435470

AGTGCTACAAGCAGGATAAGCTTTATGGCTGCCCCAAGTTCAACCTTTTTTGCTCGGGATTCCTTGCGTTTTAATATAGGTTCACATCAAACTTTGCATCGTCGAAGGGGATCAAGGTTGATAGTTAGAGCAGATGCAGACTACTACACCGTCCTTGGGGTGTCAAAAAATTCTACTAAATCTGAAATTAAGACTGCTTACCGGAAGCTAGCCCGAAATTATCATCCAGATGTGAACAAAGATCCTGGTGCAGAAGAGAAATTTAAGGAAATCAGTAATGCGTACGAGGTCTTGTCAGATGATGAGAAACGGTCCATATATGACAAATTTGGGGAGGCAGGGCTTAAAGGTTCACCGGGATTTGGTGGGGGCATGGGGGATTTCAGCAACCCTTTTGATCTGTTTGAGTCGCTATTTGAGGGCATGGATGGTCGCAGCAGACAATCTTGGAATGGAGCAATGAATGGCGAAGATGAGTATTACAGTCTTGTTTTGAACTTTAAAGAAGCTGTTTTCGGAGTGGAAAAGGAGATAGAGATTAGGCGACTAGAGAAGTGTGGTACTTGCGATGGTTCAGGAGCTAAACCAGGGACTAAATCATCCAGATGTAACACATGTGGTGGTCAGGGACGGGTTGTCACTCAAACAAGGACTCCGTTGGGTATCTTTCAGCAGTCTATGACTTGCTCGTCTTGCAATGGGACTGGAGAAACTCGAACCCCTTGCAGTACATGTTCTGGGGATGGCCGGGTGAGGAAAACAAAGCGGATAAGTCTCAAGGTTCCAGCCGGTGTGGATTCTGGTAGCCGTTTAAGGGTCCGAAATGAAGGTAACTCTGGAAAGAAAGGTGGTTCTCCTGGTGACCTCTTTGTTGTTCTTGAAGTTATCCCGGATCCCGTGCTTAAACGAGAAGACACCAACATTTTATATTCTTGTAAAGTGTCTTATATTGATGCAATCTTGGGGACTACAATTAAGGTTCCGACTGTAGATGGAATGGTGGATTTGAAAATCCCATCTGGGACACAACCAGGATCAACACTTGTTATGGCAAAGAAAGGTGTTCCTCTGCTGAATAAGAAGAATATGAGGGGTGATCAATTCGTTCGAGTGCAAGTTGAGATTCCTAAAAAACTGAGCAGCGATGAGAGAAAACTGATTGAGGAACTCTCGGAGTTGAGCAAAGGCAAGACTGCCGCCGGTATGAGATGA
Protein sequence

>Msa1435470

SATSRISFMAAPSSTFFARDSLRFNIGSHQTLHRRRGSRLIVRADADYYTVLGVSKNSTKSEIKTAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEKRSIYDKFGEAGLKGSPGFGGGMGDFSNPFDLFESLFEGMDGRSRQSWNGAMNGEDEYYSLVLNFKEAVFGVEKEIEIRRLEKCGTCDGSGAKPGTKSSRCNTCGGQGRVVTQTRTPLGIFQQSMTCSSCNGTGETRTPCSTCSGDGRVRKTKRISLKVPAGVDSGSRLRVRNEGNSGKKGGSPGDLFVVLEVIPDPVLKREDTNILYSCKVSYIDAILGTTIKVPTVDGMVDLKIPSGTQPGSTLVMAKKGVPLLNKKNMRGDQFVRVQVEIPKKLSSDERKLIEELSELSKGKTAAGMR*