AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0247020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247020 MtrunA17_Chr2g0301641 69.873 395 26 3 3 304 3 397 0.0 519
Msa0247020 MtrunA17_Chr8g0344931 35.890 326 120 7 43 282 37 359 5.52e-54 179
Msa0247020 MtrunA17_Chr8g0349331 32.551 341 147 7 6 282 10 331 1.65e-51 172
Msa0247020 MtrunA17_Chr8g0349341 32.394 355 150 6 16 282 11 363 9.23e-48 163
Msa0247020 MtrunA17_Chr8g0349321 30.997 371 149 8 6 282 6 363 3.73e-45 157
Msa0247020 MtrunA17_Chr8g0344941 36.245 229 109 3 90 282 147 374 5.04e-44 154
Msa0247020 MtrunA17_Chr8g0344941 50.704 71 34 1 26 96 24 93 6.99e-14 71.2
Msa0247020 MtrunA17_Chr8g0349301 31.498 327 118 7 5 238 5 318 1.47e-38 138
Msa0247020 MtrunA17_Chr3g0099161 25.132 378 162 12 3 282 70 424 1.37e-26 108
Msa0247020 MtrunA17_Chr5g0438641 29.052 327 133 10 31 263 22 343 6.69e-26 105
Msa0247020 MtrunA17_Chr8g0371481 28.308 325 125 11 43 267 41 357 3.32e-25 103
Msa0247020 MtrunA17_Chr5g0438631 31.980 197 104 3 99 265 146 342 1.10e-24 102
Msa0247020 MtrunA17_Chr7g0215961 24.377 361 168 9 10 282 7 350 2.54e-24 100
Msa0247020 MtrunA17_Chr8g0374101 25.000 384 172 11 2 281 2 373 2.74e-24 101
Msa0247020 MtrunA17_Chr4g0068441 27.542 236 123 6 86 282 140 366 5.57e-23 97.4
Msa0247020 MtrunA17_Chr8g0370961 31.197 234 122 6 79 281 17 242 6.40e-23 95.1
Msa0247020 MtrunA17_Chr3g0099231 26.407 231 128 5 93 286 138 363 1.57e-22 96.3
Msa0247020 MtrunA17_Chr2g0294301 26.446 242 132 6 79 282 133 366 1.64e-22 96.3
Msa0247020 MtrunA17_Chr3g0131321 25.538 372 172 13 7 281 3 366 1.62e-21 93.6
Msa0247020 MtrunA17_Chr3g0120861 31.579 190 99 3 99 258 156 344 1.80e-21 93.2
Msa0247020 MtrunA17_Chr3g0120861 51.786 56 25 1 43 98 38 91 2.25e-12 67.0
Msa0247020 MtrunA17_Chr5g0434621 28.761 226 121 7 75 266 122 341 5.68e-21 91.7
Msa0247020 MtrunA17_Chr1g0189891 23.647 351 164 9 35 288 17 360 9.81e-21 91.3
Msa0247020 MtrunA17_Chr3g0085881 26.769 325 143 13 44 281 34 350 1.20e-20 90.9
Msa0247020 MtrunA17_Chr8g0371471 28.079 203 107 5 97 265 157 354 1.38e-20 90.5
Msa0247020 MtrunA17_Chr3g0077571 25.228 329 152 10 43 281 30 354 1.50e-20 90.5
Msa0247020 MtrunA17_Chr2g0294311 24.583 240 137 5 79 282 140 371 1.68e-20 90.5
Msa0247020 MtrunA17_Chr3g0099151 28.311 219 118 7 97 281 206 419 3.89e-20 90.1
Msa0247020 MtrunA17_Chr5g0438621 30.319 188 100 4 99 256 148 334 3.99e-20 89.4
Msa0247020 MtrunA17_Chr5g0438621 38.614 101 49 4 4 103 1 89 9.22e-11 62.0
Msa0247020 MtrunA17_Chr2g0330781 29.612 206 113 4 100 274 150 354 8.82e-20 88.2
Msa0247020 MtrunA17_Chr3g0092131 28.643 199 107 6 98 265 142 336 1.14e-19 88.2
Msa0247020 MtrunA17_Chr5g0444401 25.347 288 165 9 23 281 93 359 1.69e-19 87.4
Msa0247020 MtrunA17_Chr8g0341001 28.934 197 108 4 100 265 151 346 1.06e-18 85.1
Msa0247020 MtrunA17_Chr4g0027521 26.961 204 117 3 94 265 140 343 1.41e-18 84.7
Msa0247020 MtrunA17_Chr4g0027521 55.932 59 23 2 43 100 27 83 1.76e-11 64.3
Msa0247020 MtrunA17_Chr8g0371491 25.071 351 148 11 7 258 8 342 1.77e-18 84.7
Msa0247020 MtrunA17_Chr2g0320671 29.268 205 112 4 100 273 149 351 2.06e-18 84.3
Msa0247020 MtrunA17_Chr6g0455661 26.923 234 123 6 92 281 240 469 2.55e-18 85.1
Msa0247020 MtrunA17_Chr4g0060811 28.141 199 108 6 98 265 146 340 2.59e-18 84.3
Msa0247020 MtrunA17_Chr5g0432101 28.019 207 114 4 94 265 142 348 2.60e-18 84.3
Msa0247020 MtrunA17_Chr6g0459921 27.350 234 122 7 92 281 129 358 3.15e-18 84.0
Msa0247020 MtrunA17_Chr7g0275011 26.222 225 123 5 99 287 152 369 6.31e-18 83.2
Msa0247020 MtrunA17_Chr7g0275011 47.222 72 35 2 39 109 30 99 2.43e-11 63.9
Msa0247020 MtrunA17_Chr4g0027571 27.488 211 120 4 94 273 13 221 1.49e-17 80.1
Msa0247020 MtrunA17_Chr8g0370951 27.700 213 118 6 102 282 158 366 1.58e-17 82.0
Msa0247020 MtrunA17_Chr3g0105401 27.778 198 111 4 99 265 146 342 1.81e-17 81.6
Msa0247020 MtrunA17_Chr7g0274991 25.333 225 125 4 99 287 151 368 3.28e-17 81.3
Msa0247020 MtrunA17_Chr1g0156891 27.320 194 111 4 97 261 160 352 3.48e-17 80.9
Msa0247020 MtrunA17_Chr5g0396511 28.431 204 115 5 95 268 160 362 4.53e-17 80.9
Msa0247020 MtrunA17_Chr2g0284951 26.638 229 140 6 85 288 134 359 5.37e-17 80.5
Msa0247020 MtrunA17_Chr4g0046151 28.141 199 105 5 98 259 153 350 5.76e-17 80.5
Msa0247020 MtrunA17_Chr4g0046151 56.364 55 22 1 42 96 35 87 1.79e-11 64.3
Msa0247020 MtrunA17_Chr7g0275021 26.009 223 122 6 99 285 152 367 7.31e-17 80.1
Msa0247020 MtrunA17_Chr5g0405821 25.871 201 118 4 97 267 161 360 1.92e-16 79.0
Msa0247020 MtrunA17_Chr4g0008851 27.876 226 120 8 99 287 150 369 2.28e-16 78.6
Msa0247020 MtrunA17_Chr1g0156921 28.497 193 105 6 99 260 160 350 3.09e-16 78.2
Msa0247020 MtrunA17_Chr3g0086641 26.923 234 133 9 83 281 137 367 3.18e-16 78.2
Msa0247020 MtrunA17_Chr2g0284921 24.658 219 135 6 90 281 143 358 3.27e-16 78.2
Msa0247020 MtrunA17_Chr1g0189931 27.477 222 120 7 99 285 150 365 3.79e-16 78.2
Msa0247020 MtrunA17_Chr2g0285061 25.221 226 133 7 90 281 139 362 2.02e-15 75.9
Msa0247020 MtrunA17_Chr8g0380031 24.186 215 136 5 91 281 192 403 2.10e-15 76.3
Msa0247020 MtrunA17_Chr4g0017431 24.554 224 135 7 90 281 139 360 3.43e-15 75.1
Msa0247020 MtrunA17_Chr1g0156901 24.528 212 118 6 91 265 156 362 3.79e-15 75.1
Msa0247020 MtrunA17_Chr2g0285001 26.923 234 138 8 85 288 135 365 6.03e-15 74.7
Msa0247020 MtrunA17_Chr3g0142921 25.352 213 123 6 88 265 138 349 1.25e-14 73.6
Msa0247020 MtrunA17_Chr6g0457921 25.229 218 130 5 99 285 148 363 1.55e-14 73.2
Msa0247020 MtrunA17_Chr2g0284991 25.688 218 128 6 91 279 141 353 1.58e-14 73.2
Msa0247020 MtrunA17_Chr8g0380041 25.333 225 132 8 91 281 138 360 2.95e-14 72.4
Msa0247020 MtrunA17_Chr2g0283191 26.531 196 109 5 99 259 159 354 3.08e-14 72.4
Msa0247020 MtrunA17_Chr1g0156911 23.684 190 115 4 99 259 155 343 9.92e-14 70.9
Msa0247020 MtrunA17_Chr8g0370981 35.878 131 77 3 97 225 166 291 1.71e-13 70.5
Msa0247020 MtrunA17_Chr1g0157001 28.571 203 105 7 90 259 146 341 3.04e-13 69.7
Msa0247020 MtrunA17_Chr3g0142941 24.885 217 128 8 90 274 152 365 1.37e-12 67.4
Msa0247020 MtrunA17_Chr1g0156931 25.490 204 105 8 99 265 159 352 2.01e-11 63.9
Msa0247020 MtrunA17_Chr3g0085231 25.000 212 135 5 91 281 139 347 3.58e-11 63.2
Msa0247020 MtrunA17_Chr8g0352901 26.512 215 112 8 98 269 149 360 4.69e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247020 AT1G53920.1 31.915 376 148 10 7 282 15 382 2.76e-49 168
Msa0247020 AT3G14225.1 35.616 219 105 3 99 282 158 375 1.32e-37 137
Msa0247020 AT3G14225.2 35.616 219 105 3 99 282 154 371 1.62e-37 137
Msa0247020 AT1G53940.2 28.804 368 161 8 15 282 7 373 3.11e-37 136
Msa0247020 AT5G40990.1 34.545 220 108 3 98 282 153 371 7.05e-35 130
Msa0247020 AT1G53990.1 33.182 220 111 3 98 282 144 362 1.56e-33 126
Msa0247020 AT1G54030.1 26.019 319 150 9 42 282 51 361 1.24e-28 114
Msa0247020 AT1G71120.1 29.439 214 118 5 105 288 152 362 6.32e-27 108
Msa0247020 AT1G54020.2 26.646 319 147 9 44 281 34 346 4.81e-24 100
Msa0247020 AT2G23540.1 28.761 226 116 5 95 281 165 384 2.28e-23 99.4
Msa0247020 AT1G53940.1 31.174 247 105 6 39 222 34 278 4.25e-23 97.1
Msa0247020 AT1G71691.1 27.966 236 129 6 84 281 47 279 1.86e-22 95.1
Msa0247020 AT3G14210.1 25.000 332 155 8 38 281 31 356 5.51e-22 95.5
Msa0247020 AT4G10950.1 24.556 338 151 10 43 284 71 400 7.48e-22 95.1
Msa0247020 AT1G71691.2 28.774 212 118 5 100 281 172 380 8.12e-22 94.7
Msa0247020 AT3G50400.1 28.814 236 120 7 86 281 144 371 1.15e-21 94.4
Msa0247020 AT2G42990.1 29.064 203 109 5 99 267 146 347 1.64e-21 93.6
Msa0247020 AT3G53100.1 30.435 207 113 4 97 273 145 350 1.73e-21 93.6
Msa0247020 AT1G54010.1 24.932 369 170 13 2 281 4 354 2.10e-21 93.6
Msa0247020 AT1G54000.1 26.140 329 143 11 43 281 36 354 2.20e-21 93.6
Msa0247020 AT5G03820.1 29.293 198 109 3 99 265 151 348 4.62e-21 92.4
Msa0247020 AT5G03820.2 29.293 198 109 3 99 265 179 376 7.58e-21 92.0
Msa0247020 AT5G08460.1 30.126 239 123 7 85 281 146 382 7.81e-21 92.0
Msa0247020 AT1G54030.2 27.586 232 109 7 42 222 51 274 1.28e-20 90.5
Msa0247020 AT5G22810.1 29.358 218 114 6 94 273 143 358 1.46e-20 90.9
Msa0247020 AT5G03810.1 28.426 197 111 2 99 265 148 344 2.36e-20 90.1
Msa0247020 AT4G26790.1 32.979 188 95 4 99 256 147 333 2.53e-20 90.1
Msa0247020 AT4G26790.1 55.556 63 25 2 43 104 29 89 7.15e-11 62.8
Msa0247020 AT4G26790.2 32.979 188 95 4 99 256 147 333 2.53e-20 90.1
Msa0247020 AT4G26790.2 55.556 63 25 2 43 104 29 89 7.15e-11 62.8
Msa0247020 AT2G19010.2 24.251 367 179 14 6 281 17 375 6.60e-20 89.4
Msa0247020 AT2G19010.1 25.387 323 148 11 44 281 31 345 1.20e-19 88.2
Msa0247020 AT3G14220.1 25.231 325 149 11 44 281 33 350 1.96e-19 87.8
Msa0247020 AT3G16370.1 30.047 213 117 5 93 274 142 353 4.13e-19 86.7
Msa0247020 AT5G33370.1 26.457 223 121 4 99 285 148 363 1.08e-18 85.9
Msa0247020 AT5G15720.1 25.073 343 145 11 43 281 30 364 1.12e-18 85.5
Msa0247020 AT5G37690.2 25.628 199 114 4 98 266 137 331 1.40e-18 85.1
Msa0247020 AT5G37690.1 25.628 199 114 4 98 266 145 339 1.45e-18 85.1
Msa0247020 AT4G10950.2 25.664 226 126 5 95 284 185 404 1.48e-18 85.5
Msa0247020 AT1G75890.3 29.665 209 108 6 90 262 182 387 3.39e-18 84.7
Msa0247020 AT1G75890.2 29.187 209 109 6 90 262 153 358 3.52e-18 84.3
Msa0247020 AT1G75890.1 29.665 209 108 6 90 262 166 371 3.63e-18 84.3
Msa0247020 AT1G06990.1 26.027 219 125 6 74 255 123 341 7.49e-18 83.2
Msa0247020 AT1G74460.1 27.861 201 110 6 96 265 140 336 9.16e-18 83.2
Msa0247020 AT2G04570.1 27.000 200 115 3 100 269 147 345 1.20e-17 82.4
Msa0247020 AT2G04570.1 52.542 59 25 2 43 100 28 84 1.46e-11 64.7
Msa0247020 AT5G63170.1 31.868 182 92 5 101 258 149 322 3.25e-17 81.3
Msa0247020 AT1G20135.1 29.744 195 98 4 95 259 139 324 6.50e-17 80.1
Msa0247020 AT1G29670.1 25.333 225 131 6 91 281 139 360 2.36e-16 79.0
Msa0247020 AT1G29670.2 25.333 225 131 6 91 281 207 428 5.59e-16 78.2
Msa0247020 AT3G14210.2 27.014 211 113 5 108 281 22 228 5.63e-16 76.6
Msa0247020 AT5G45960.1 26.761 213 121 5 98 276 164 375 5.67e-16 77.8
Msa0247020 AT5G45960.1 50.769 65 30 1 32 96 36 98 7.98e-12 65.9
Msa0247020 AT1G20120.1 28.500 200 108 6 94 259 187 385 6.76e-16 77.8
Msa0247020 AT5G15720.3 25.882 255 135 8 71 281 89 333 1.11e-15 76.6
Msa0247020 AT3G04290.1 25.806 217 118 4 99 279 147 356 1.42e-15 76.6
Msa0247020 AT1G58520.3 27.368 190 113 4 92 259 141 327 3.55e-15 75.5
Msa0247020 AT1G58525.1 27.368 190 113 4 92 259 141 327 3.55e-15 75.5
Msa0247020 AT4G18970.1 23.795 332 155 10 45 281 30 358 4.06e-15 75.5
Msa0247020 AT5G18430.1 25.740 338 150 14 39 279 24 357 4.24e-15 75.5
Msa0247020 AT5G45950.1 22.396 192 119 3 99 263 157 345 4.31e-15 75.1
Msa0247020 AT5G45670.1 23.353 334 154 12 45 281 31 359 4.93e-15 75.1
Msa0247020 AT1G29660.1 26.068 234 136 8 83 281 130 361 5.43e-15 75.1
Msa0247020 AT4G18970.2 23.795 332 155 10 45 281 30 358 6.22e-15 75.1
Msa0247020 AT1G75880.1 27.143 210 114 6 94 267 162 368 7.16e-15 74.7
Msa0247020 AT1G75880.2 27.143 210 114 6 94 267 161 367 7.33e-15 74.7
Msa0247020 AT5G15720.2 26.749 243 108 8 43 218 30 269 8.40e-14 71.2
Msa0247020 AT1G53970.2 56.140 57 24 1 42 98 19 74 8.60e-14 66.6
Msa0247020 AT1G53970.1 56.140 57 24 1 42 98 19 74 8.60e-14 66.6
Msa0247020 AT1G71691.3 27.542 236 105 8 43 216 55 286 8.82e-14 70.9
Msa0247020 AT1G23500.1 28.571 189 109 5 101 268 156 339 1.30e-13 70.9
Msa0247020 AT1G59406.1 25.888 197 114 4 92 259 141 334 1.81e-13 70.5
Msa0247020 AT1G59030.1 25.888 197 114 4 92 259 141 334 1.81e-13 70.5
Msa0247020 AT1G58725.1 25.888 197 114 4 92 259 141 334 1.81e-13 70.5
Msa0247020 AT5G55050.1 23.478 230 142 6 83 281 147 373 3.08e-13 70.1
Msa0247020 AT5G42170.1 26.396 197 111 4 99 265 168 360 7.68e-13 68.6
Msa0247020 AT3G27950.1 27.885 208 106 6 103 271 158 360 2.68e-12 67.0
Msa0247020 AT2G24560.1 25.123 203 118 5 90 258 140 342 2.70e-12 67.0
Msa0247020 AT1G20132.1 26.540 211 116 5 90 266 170 375 5.56e-12 66.2
Msa0247020 AT1G58430.1 24.873 197 118 4 99 265 154 350 9.70e-12 65.5
Msa0247020 AT1G75900.1 25.472 212 114 8 94 267 152 357 4.60e-11 63.5
Msa0247020 AT4G28780.1 37.736 106 53 4 5 109 3 96 7.30e-11 62.8
Msa0247020 AT3G14820.1 26.263 198 113 5 97 265 148 341 9.09e-11 62.4
Msa0247020 AT3G43570.1 22.727 176 117 3 92 259 141 305 9.87e-11 62.0

Find 61 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTGTACATGAACCCTTTA+AGG 0.083860 2_2:-28904151 None:intergenic
CAGAATCCATGCTCAATTTC+TGG 0.160420 2_2:-28904857 None:intergenic
AGCAATTTGCAAAGGCTTTA+TGG 0.186765 2_2:+28904741 Msa0247020:CDS
TGTAAACCATATGGACAAAA+TGG 0.193884 2_2:+28902496 Msa0247020:CDS
GAGAAAGGAGCAAGAAAATT+TGG 0.199229 2_2:+28903978 Msa0247020:CDS
TACAATCCTGAACAATATAT+TGG 0.247624 2_2:+28903658 Msa0247020:CDS
CTTCAGGATTTGCAGCTCTA+AGG 0.265871 2_2:-28904037 None:intergenic
AGCCTCAACCAAGTCCTTAA+AGG 0.288242 2_2:+28904137 Msa0247020:CDS
CAACCACCTTTGTTTGCTTC+AGG 0.296262 2_2:-28904053 None:intergenic
TAAATTGTATGGTCCAACTA+TGG 0.310691 2_2:-28904777 None:intergenic
GGTGACTCTACTGTTGATTC+TGG 0.322268 2_2:+28902430 Msa0247020:CDS
AATAGCCTTTCTAAATTGTA+TGG 0.346425 2_2:-28904788 None:intergenic
CAATATATTGGGATGGTCAT+TGG 0.379122 2_2:+28903670 Msa0247020:CDS
ACCATCCCAATATATTGTTC+AGG 0.382279 2_2:-28903664 None:intergenic
CATTGGAAGCAATGATTACA+TGG 0.401728 2_2:+28903600 Msa0247020:CDS
GTAAACCATATGGACAAAAT+GGG 0.407821 2_2:+28902497 Msa0247020:CDS
ACAATCCTGAACAATATATT+GGG 0.421945 2_2:+28903659 Msa0247020:CDS
GCTCATGAATCTTCTCTGTT+GGG 0.437327 2_2:-28904721 None:intergenic
AGAATCCATGCTCAATTTCT+GGG 0.441546 2_2:-28904856 None:intergenic
TGCTCATGAATCTTCTCTGT+TGG 0.444342 2_2:-28904722 None:intergenic
GCCTCAACCAAGTCCTTAAA+GGG 0.453919 2_2:+28904138 Msa0247020:CDS
GGCATTATTGGTAATACCAT+TGG 0.454625 2_2:-28902395 None:intergenic
GCAGTTTATTTCATTAGCAT+TGG 0.455789 2_2:+28903583 Msa0247020:CDS
TTCAGGATTTGCAGCTCTAA+GGG 0.463647 2_2:-28904036 None:intergenic
AAAGCAGATTGTAAACCATA+TGG 0.466830 2_2:+28902487 Msa0247020:CDS
TTTGCAAAGGCTTTATGGAA+TGG 0.478872 2_2:+28904746 Msa0247020:CDS
GGATTTGCAGCTCTAAGGGC+TGG 0.481321 2_2:-28904032 None:intergenic
ACATGAACCCTTTAAGGACT+TGG 0.482803 2_2:-28904145 None:intergenic
TTGGACCATACAATTTAGAA+AGG 0.487352 2_2:+28904783 Msa0247020:CDS
AATGGATCACCTTCCATAGT+TGG 0.487577 2_2:+28904764 Msa0247020:CDS
ACCCTTTAAGGACTTGGTTG+AGG 0.492760 2_2:-28904139 None:intergenic
TGTTGTGGAAGTGGACCATA+TGG 0.496313 2_2:+28904608 Msa0247020:intron
ACAATGTTGAAGAGTTTGTA+TGG 0.508382 2_2:+28904684 Msa0247020:CDS
CCCTCAGGACGATTCTCAAA+TGG 0.515861 2_2:+28902532 Msa0247020:CDS
GATTCATGAGCAATTTGCAA+AGG 0.535695 2_2:+28904733 Msa0247020:CDS
TGTTGAAGAGTTTGTATGGT+GGG 0.545215 2_2:+28904688 Msa0247020:CDS
TTACTTGAATTGCTTGTGTG+AGG 0.550446 2_2:-28903696 None:intergenic
AGAAAAGCTTGGAGAGGAGA+AGG 0.560151 2_2:+28903540 Msa0247020:intron
CCATTTGAGAATCGTCCTGA+GGG 0.561496 2_2:-28902532 None:intergenic
AAATCACTAGCAGAAAAGCT+TGG 0.565028 2_2:+28903529 Msa0247020:intron
TTGCAGCTCTAAGGGCTGGT+AGG 0.572109 2_2:-28904028 None:intergenic
TTGTATGGTCCAACTATGGA+AGG 0.576227 2_2:-28904773 None:intergenic
CTATAAAATCTGTAATAACA+CGG 0.585420 2_2:-28902555 None:intergenic
CATGAATCTTCTCTGTTGGG+TGG 0.589287 2_2:-28904718 None:intergenic
GGAAGCAATGATTACATGGG+TGG 0.592304 2_2:+28903604 Msa0247020:CDS
GTACAGATACTATATGAGAA+AGG 0.594863 2_2:+28903963 Msa0247020:intron
CATGTGTAGATACCTCCATA+TGG 0.597763 2_2:-28904623 None:intergenic
TCCTGAACAATATATTGGGA+TGG 0.604971 2_2:+28903663 Msa0247020:CDS
TAAACCATATGGACAAAATG+GGG 0.612650 2_2:+28902498 Msa0247020:CDS
GATGTCCCAGAAATTGAGCA+TGG 0.616298 2_2:+28904851 Msa0247020:CDS
GCCATTTGAGAATCGTCCTG+AGG 0.622096 2_2:-28902533 None:intergenic
AATCCTGAAGCAAACAAAGG+TGG 0.650763 2_2:+28904050 Msa0247020:CDS
ACTAGCAGAAAAGCTTGGAG+AGG 0.654666 2_2:+28903534 Msa0247020:intron
GCTTGGAGAGGAGAAGGCAA+AGG 0.655831 2_2:+28903546 Msa0247020:CDS
GGAGGTATCTACACATGTGG+TGG 0.666558 2_2:+28904629 Msa0247020:CDS
GCAAATCCTGAAGCAAACAA+AGG 0.677622 2_2:+28904047 Msa0247020:CDS
AGGGCTGGTAGGCAACCCAA+AGG 0.678547 2_2:-28904017 None:intergenic
TATGGAGGTATCTACACATG+TGG 0.687712 2_2:+28904626 Msa0247020:CDS
ATGTTGAAGAGTTTGTATGG+TGG 0.689773 2_2:+28904687 Msa0247020:CDS
ATTGGAAGCAATGATTACAT+GGG 0.691784 2_2:+28903601 Msa0247020:CDS
TGTGGAAGTGGACCATATGG+AGG 0.726817 2_2:+28904611 Msa0247020:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACTAATTTATGTTTAAA+TGG + chr2_2:28903391-28903410 Msa0247020:intron 10.0%
!! TAATTCAACATAAATTAAAT+AGG + chr2_2:28903462-28903481 Msa0247020:intron 10.0%
!!! ACAAAAAATTATAACTATTT+TGG - chr2_2:28903770-28903789 None:intergenic 10.0%
!!! ATATTCTCGATTTTTTTTTA+AGG + chr2_2:28902898-28902917 Msa0247020:intron 15.0%
!!! GAATTTTATCATTGATTTTT+AGG + chr2_2:28902931-28902950 Msa0247020:intron 15.0%
!!! AAAAGCTTGTTTAATATAAA+AGG + chr2_2:28903425-28903444 Msa0247020:intron 15.0%
!! GTTATTTAATTAAAATGTTC+AGG + chr2_2:28903826-28903845 Msa0247020:intron 15.0%
!! TTGATTATATCATTTCTTTA+AGG - chr2_2:28903927-28903946 None:intergenic 15.0%
!! GAATATTGACATATAATAAA+TGG - chr2_2:28904246-28904265 None:intergenic 15.0%
!! TGAAAAACAAAAAAATTGTT+TGG - chr2_2:28904559-28904578 None:intergenic 15.0%
!!! ATTTTTTTGTTTTTCAATGA+AGG + chr2_2:28904563-28904582 Msa0247020:intron 15.0%
!! CTATAAAATCTGTAATAACA+CGG - chr2_2:28902558-28902577 None:intergenic 20.0%
!!! ATAGTGTTCAAAGAATTTTA+TGG + chr2_2:28902864-28902883 Msa0247020:intron 20.0%
!! AAAGGACAAAGTATATTAAT+TGG - chr2_2:28902957-28902976 None:intergenic 20.0%
!! TAATTATATGTATCGTTGTA+GGG - chr2_2:28903018-28903037 None:intergenic 20.0%
!!! TCAATAGATGGTTTTAAAAA+TGG - chr2_2:28903186-28903205 None:intergenic 20.0%
!! CAATTACTATAATCAATAGA+TGG - chr2_2:28903198-28903217 None:intergenic 20.0%
!!! TTTGTATTTTAGTTAGTTGA+AGG - chr2_2:28903872-28903891 None:intergenic 20.0%
!! CAAATTCTAATTTCTATGAT+TGG + chr2_2:28904171-28904190 Msa0247020:intron 20.0%
!! ATAAATAATCCCACAAAATA+TGG + chr2_2:28904206-28904225 Msa0247020:intron 20.0%
!!! AAAAATGAAAAGGGCATTAT+TGG - chr2_2:28902410-28902429 None:intergenic 25.0%
!! AATAATGCCCTTTTCATTTT+TGG + chr2_2:28902409-28902428 Msa0247020:CDS 25.0%
!!! CGTGTTATTACAGATTTTAT+AGG + chr2_2:28902556-28902575 Msa0247020:CDS 25.0%
!!! CAATTCGTTGTTTTGAATTT+GGG + chr2_2:28902634-28902653 Msa0247020:intron 25.0%
!!! AATTCGTTGTTTTGAATTTG+GGG + chr2_2:28902635-28902654 Msa0247020:intron 25.0%
! CTTTATATTGTAGTAAGATG+TGG + chr2_2:28902688-28902707 Msa0247020:intron 25.0%
! GCTACAATATAAAAGTTCAA+AGG - chr2_2:28902764-28902783 None:intergenic 25.0%
! GTAATTATATGTATCGTTGT+AGG - chr2_2:28903019-28903038 None:intergenic 25.0%
!!! ATAGATGGTTTTAAAAATGG+AGG - chr2_2:28903183-28903202 None:intergenic 25.0%
! ATGCTTAAACAATATGTGTA+AGG - chr2_2:28903281-28903300 None:intergenic 25.0%
!! GCATTTGATAAACATGTTTT+AGG + chr2_2:28903347-28903366 Msa0247020:intron 25.0%
! TCAAAGTTACTTAATTGTGT+TGG - chr2_2:28903499-28903518 None:intergenic 25.0%
! TACAATCCTGAACAATATAT+TGG + chr2_2:28903658-28903677 Msa0247020:CDS 25.0%
! ACAATCCTGAACAATATATT+GGG + chr2_2:28903659-28903678 Msa0247020:CDS 25.0%
!!! ATTTTTAAGTTTGTCTCCTT+TGG + chr2_2:28904001-28904020 Msa0247020:CDS 25.0%
!!! TTTTTAAGTTTGTCTCCTTT+GGG + chr2_2:28904002-28904021 Msa0247020:CDS 25.0%
!! AAAGACATACCATATTTTGT+GGG - chr2_2:28904218-28904237 None:intergenic 25.0%
! TTGGTTATTTGTATTTGCAT+CGG + chr2_2:28904516-28904535 Msa0247020:intron 25.0%
! AATAGCCTTTCTAAATTGTA+TGG - chr2_2:28904791-28904810 None:intergenic 25.0%
CACAAAAATGAAATTGCCAA+TGG + chr2_2:28902379-28902398 Msa0247020:CDS 30.0%
AGAGTCACCAAAAATGAAAA+GGG - chr2_2:28902419-28902438 None:intergenic 30.0%
TAGAGTCACCAAAAATGAAA+AGG - chr2_2:28902420-28902439 None:intergenic 30.0%
!!! TTACAATCTGCTTTGTTTTC+AGG - chr2_2:28902481-28902500 None:intergenic 30.0%
AAAGCAGATTGTAAACCATA+TGG + chr2_2:28902487-28902506 Msa0247020:CDS 30.0%
TGTAAACCATATGGACAAAA+TGG + chr2_2:28902496-28902515 Msa0247020:CDS 30.0%
GTAAACCATATGGACAAAAT+GGG + chr2_2:28902497-28902516 Msa0247020:CDS 30.0%
TAAACCATATGGACAAAATG+GGG + chr2_2:28902498-28902517 Msa0247020:CDS 30.0%
GAAAAAAAAGATAGTCACAG+TGG - chr2_2:28902593-28902612 None:intergenic 30.0%
!!! GCAATTCGTTGTTTTGAATT+TGG + chr2_2:28902633-28902652 Msa0247020:intron 30.0%
TACGAGACACTTAATATTGA+AGG + chr2_2:28902981-28903000 Msa0247020:intron 30.0%
TCCATATTTGTATGCTTCAT+AGG + chr2_2:28903088-28903107 Msa0247020:intron 30.0%
ACCTATGAAGCATACAAATA+TGG - chr2_2:28903092-28903111 None:intergenic 30.0%
TTTAAAAATGGAGGAATCAG+AGG - chr2_2:28903174-28903193 None:intergenic 30.0%
! TAAGTAACTTTGAAGAAGTG+AGG + chr2_2:28903506-28903525 Msa0247020:intron 30.0%
GCAGTTTATTTCATTAGCAT+TGG + chr2_2:28903583-28903602 Msa0247020:CDS 30.0%
ATTGGAAGCAATGATTACAT+GGG + chr2_2:28903601-28903620 Msa0247020:CDS 30.0%
! TGTAGCTTTCTTGCATTTTT+GGG - chr2_2:28903642-28903661 None:intergenic 30.0%
! TTGTAGCTTTCTTGCATTTT+TGG - chr2_2:28903643-28903662 None:intergenic 30.0%
GTACAGATACTATATGAGAA+AGG + chr2_2:28903963-28903982 Msa0247020:intron 30.0%
! GAAAGACATACCATATTTTG+TGG - chr2_2:28904219-28904238 None:intergenic 30.0%
! TTTCAGATTGAAGGTGTATT+CGG + chr2_2:28904294-28904313 Msa0247020:intron 30.0%
! ACATCTTCAATCTGAAATGT+CGG - chr2_2:28904393-28904412 None:intergenic 30.0%
GTATCTGATATTGATACATG+TGG + chr2_2:28904435-28904454 Msa0247020:intron 30.0%
TCAACATGTCAATATCATGT+TGG + chr2_2:28904497-28904516 Msa0247020:intron 30.0%
!! GTTTTTCAATGAAGGTTTTG+AGG + chr2_2:28904571-28904590 Msa0247020:intron 30.0%
ACAATGTTGAAGAGTTTGTA+TGG + chr2_2:28904684-28904703 Msa0247020:CDS 30.0%
TAAATTGTATGGTCCAACTA+TGG - chr2_2:28904780-28904799 None:intergenic 30.0%
TTGGACCATACAATTTAGAA+AGG + chr2_2:28904783-28904802 Msa0247020:CDS 30.0%
! GGCATTATTGGTAATACCAT+TGG - chr2_2:28902398-28902417 None:intergenic 35.0%
! AAAACCCCATTTTGTCCATA+TGG - chr2_2:28902505-28902524 None:intergenic 35.0%
ATATAAAGAGACCATGAGAC+TGG + chr2_2:28902824-28902843 Msa0247020:intron 35.0%
TTAAGTGTCTCGTACACAAA+AGG - chr2_2:28902975-28902994 None:intergenic 35.0%
AAATCACTAGCAGAAAAGCT+TGG + chr2_2:28903529-28903548 Msa0247020:intron 35.0%
CATTGGAAGCAATGATTACA+TGG + chr2_2:28903600-28903619 Msa0247020:CDS 35.0%
TCCTGAACAATATATTGGGA+TGG + chr2_2:28903663-28903682 Msa0247020:CDS 35.0%
ACCATCCCAATATATTGTTC+AGG - chr2_2:28903667-28903686 None:intergenic 35.0%
CAATATATTGGGATGGTCAT+TGG + chr2_2:28903670-28903689 Msa0247020:CDS 35.0%
TTACTTGAATTGCTTGTGTG+AGG - chr2_2:28903699-28903718 None:intergenic 35.0%
GAGAAAGGAGCAAGAAAATT+TGG + chr2_2:28903978-28903997 Msa0247020:CDS 35.0%
TTGTGTACATGAACCCTTTA+AGG - chr2_2:28904154-28904173 None:intergenic 35.0%
! ACACCTTCAATCTGAAATGT+CGG - chr2_2:28904291-28904310 None:intergenic 35.0%
GTATCTAATACTGAGACATG+TGG + chr2_2:28904333-28904352 Msa0247020:intron 35.0%
!!! TTCAATGAAGGTTTTGAGGA+TGG + chr2_2:28904575-28904594 Msa0247020:intron 35.0%
!!! TCAATGAAGGTTTTGAGGAT+GGG + chr2_2:28904576-28904595 Msa0247020:intron 35.0%
ATGTTGAAGAGTTTGTATGG+TGG + chr2_2:28904687-28904706 Msa0247020:CDS 35.0%
TGTTGAAGAGTTTGTATGGT+GGG + chr2_2:28904688-28904707 Msa0247020:CDS 35.0%
GATTCATGAGCAATTTGCAA+AGG + chr2_2:28904733-28904752 Msa0247020:CDS 35.0%
AGCAATTTGCAAAGGCTTTA+TGG + chr2_2:28904741-28904760 Msa0247020:CDS 35.0%
! TTTGCAAAGGCTTTATGGAA+TGG + chr2_2:28904746-28904765 Msa0247020:CDS 35.0%
AGAATCCATGCTCAATTTCT+GGG - chr2_2:28904859-28904878 None:intergenic 35.0%
TGACATCACAAGAGCAATTG+TGG - chr2_2:28902733-28902752 None:intergenic 40.0%
!! AGACTGGTCTCTATACTCTT+TGG + chr2_2:28902840-28902859 Msa0247020:intron 40.0%
CTTCATAGGTATGACATGTG+AGG + chr2_2:28903102-28903121 Msa0247020:intron 40.0%
TGTGTCAACTTTGCTTCTAG+TGG + chr2_2:28903216-28903235 Msa0247020:intron 40.0%
GTGTCAACTTTGCTTCTAGT+GGG + chr2_2:28903217-28903236 Msa0247020:intron 40.0%
TGTCAACTTTGCTTCTAGTG+GGG + chr2_2:28903218-28903237 Msa0247020:intron 40.0%
TGTTCTTCCTCAAACCAATC+AGG + chr2_2:28903245-28903264 Msa0247020:intron 40.0%
GTTCTTCCTCAAACCAATCA+GGG + chr2_2:28903246-28903265 Msa0247020:intron 40.0%
! AGGTGATTTTGCAGCTACTT+AGG - chr2_2:28903852-28903871 None:intergenic 40.0%
! TTCAGGATTTGCAGCTCTAA+GGG - chr2_2:28904039-28904058 None:intergenic 40.0%
GCAAATCCTGAAGCAAACAA+AGG + chr2_2:28904047-28904066 Msa0247020:CDS 40.0%
AATCCTGAAGCAAACAAAGG+TGG + chr2_2:28904050-28904069 Msa0247020:CDS 40.0%
! ACATGAACCCTTTAAGGACT+TGG - chr2_2:28904148-28904167 None:intergenic 40.0%
ACACCGACATTTCAGATTGA+AGG + chr2_2:28904285-28904304 Msa0247020:intron 40.0%
!!! CAATGAAGGTTTTGAGGATG+GGG + chr2_2:28904577-28904596 Msa0247020:intron 40.0%
!! GTTAATGCTTGTTGTGGAAG+TGG + chr2_2:28904599-28904618 Msa0247020:intron 40.0%
CATGTGTAGATACCTCCATA+TGG - chr2_2:28904626-28904645 None:intergenic 40.0%
!! TATGGAGGTATCTACACATG+TGG + chr2_2:28904626-28904645 Msa0247020:CDS 40.0%
GCTCATGAATCTTCTCTGTT+GGG - chr2_2:28904724-28904743 None:intergenic 40.0%
TGCTCATGAATCTTCTCTGT+TGG - chr2_2:28904725-28904744 None:intergenic 40.0%
AATGGATCACCTTCCATAGT+TGG + chr2_2:28904764-28904783 Msa0247020:CDS 40.0%
TTGTATGGTCCAACTATGGA+AGG - chr2_2:28904776-28904795 None:intergenic 40.0%
CAGAATCCATGCTCAATTTC+TGG - chr2_2:28904860-28904879 None:intergenic 40.0%
!! GGTGACTCTACTGTTGATTC+TGG + chr2_2:28902430-28902449 Msa0247020:CDS 45.0%
! GGGGTTTTTGATAAACCCTC+AGG + chr2_2:28902517-28902536 Msa0247020:CDS 45.0%
CCATTTGAGAATCGTCCTGA+GGG - chr2_2:28902535-28902554 None:intergenic 45.0%
GAGTATAGAGACCAGTCTCA+TGG - chr2_2:28902838-28902857 None:intergenic 45.0%
ACTAGCAGAAAAGCTTGGAG+AGG + chr2_2:28903534-28903553 Msa0247020:intron 45.0%
AGAAAAGCTTGGAGAGGAGA+AGG + chr2_2:28903540-28903559 Msa0247020:intron 45.0%
GGAAGCAATGATTACATGGG+TGG + chr2_2:28903604-28903623 Msa0247020:CDS 45.0%
CTTCAGGATTTGCAGCTCTA+AGG - chr2_2:28904040-28904059 None:intergenic 45.0%
! CAACCACCTTTGTTTGCTTC+AGG - chr2_2:28904056-28904075 None:intergenic 45.0%
AGCCTCAACCAAGTCCTTAA+AGG + chr2_2:28904137-28904156 Msa0247020:CDS 45.0%
GCCTCAACCAAGTCCTTAAA+GGG + chr2_2:28904138-28904157 Msa0247020:CDS 45.0%
! ACCCTTTAAGGACTTGGTTG+AGG - chr2_2:28904142-28904161 None:intergenic 45.0%
! GATGGGGTTAATGCTTGTTG+TGG + chr2_2:28904593-28904612 Msa0247020:intron 45.0%
TGTTGTGGAAGTGGACCATA+TGG + chr2_2:28904608-28904627 Msa0247020:intron 45.0%
CATGAATCTTCTCTGTTGGG+TGG - chr2_2:28904721-28904740 None:intergenic 45.0%
GATGTCCCAGAAATTGAGCA+TGG + chr2_2:28904851-28904870 Msa0247020:CDS 45.0%
!! TAATTACATTTAATTATTAT+TGG + chr2_2:28903032-28903051 Msa0247020:intron 5.0%
!!! ATTCTTTATTAAATTTTTAT+TGG + chr2_2:28903735-28903754 Msa0247020:intron 5.0%
CCCTCAGGACGATTCTCAAA+TGG + chr2_2:28902532-28902551 Msa0247020:CDS 50.0%
GCCATTTGAGAATCGTCCTG+AGG - chr2_2:28902536-28902555 None:intergenic 50.0%
GTGGACAAATGTGACCGATG+CGG + chr2_2:28902707-28902726 Msa0247020:intron 50.0%
AAGAGCAATTGTGGCCGCAT+CGG - chr2_2:28902724-28902743 None:intergenic 50.0%
AACTTTGCTTCTAGTGGGGC+TGG + chr2_2:28903222-28903241 Msa0247020:intron 50.0%
TCCTCAAACCAATCAGGGAC+TGG + chr2_2:28903251-28903270 Msa0247020:intron 50.0%
!! ACCAGTCCCTGATTGGTTTG+AGG - chr2_2:28903255-28903274 None:intergenic 50.0%
AAGGACTACCAGTCCCTGAT+TGG - chr2_2:28903262-28903281 None:intergenic 50.0%
TGTGGAAGTGGACCATATGG+AGG + chr2_2:28904611-28904630 Msa0247020:intron 50.0%
!! GGAGGTATCTACACATGTGG+TGG + chr2_2:28904629-28904648 Msa0247020:CDS 50.0%
GCTTGGAGAGGAGAAGGCAA+AGG + chr2_2:28903546-28903565 Msa0247020:CDS 55.0%
!! TTGCAGCTCTAAGGGCTGGT+AGG - chr2_2:28904031-28904050 None:intergenic 55.0%
!! GGATTTGCAGCTCTAAGGGC+TGG - chr2_2:28904035-28904054 None:intergenic 55.0%
! AGGGCTGGTAGGCAACCCAA+AGG - chr2_2:28904020-28904039 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 28902289 28904880 28902289 ID=Msa0247020;Name=Msa0247020
chr2_2 mRNA 28902289 28904880 28902289 ID=Msa0247020-mRNA-1;Parent=Msa0247020;Name=Msa0247020-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|4|0|304
chr2_2 exon 28902289 28902577 28902289 ID=Msa0247020-mRNA-1:exon:10332;Parent=Msa0247020-mRNA-1
chr2_2 exon 28903542 28903714 28903542 ID=Msa0247020-mRNA-1:exon:10333;Parent=Msa0247020-mRNA-1
chr2_2 exon 28903969 28904159 28903969 ID=Msa0247020-mRNA-1:exon:10334;Parent=Msa0247020-mRNA-1
chr2_2 exon 28904619 28904880 28904619 ID=Msa0247020-mRNA-1:exon:10335;Parent=Msa0247020-mRNA-1
chr2_2 CDS 28902289 28902577 28902289 ID=Msa0247020-mRNA-1:cds;Parent=Msa0247020-mRNA-1
chr2_2 CDS 28903542 28903714 28903542 ID=Msa0247020-mRNA-1:cds;Parent=Msa0247020-mRNA-1
chr2_2 CDS 28903969 28904159 28903969 ID=Msa0247020-mRNA-1:cds;Parent=Msa0247020-mRNA-1
chr2_2 CDS 28904619 28904880 28904619 ID=Msa0247020-mRNA-1:cds;Parent=Msa0247020-mRNA-1
Gene Sequence

>Msa0247020

AATCAAAGTCTAAATTTTTTGTGTTGTTTTATTGTTTACATAGTTTTAATTCAATTCATTGCTTCATTGAGTTTGATCACATCACAAAGTCACAAAAATGAAATTGCCAATGGTATTACCAATAATGCCCTTTTCATTTTTGGTGACTCTACTGTTGATTCTGGCAACAACAACTATATAGATACTATTCCTGAAAACAAAGCAGATTGTAAACCATATGGACAAAATGGGGTTTTTGATAAACCCTCAGGACGATTCTCAAATGGCCGTGTTATTACAGATTTTATAGAAAAGCTTGGAGAGGAGAAGGCAAAGGATTTGATCTCAGAAGCAGTTTATTTCATTAGCATTGGAAGCAATGATTACATGGGTGGTTATTTAAATAACCCAAAAATGCAAGAAAGCTACAATCCTGAACAATATATTGGGATGGTCATTGGAAACCTCACACAAGCAATTCAAATACTATATGAGAAAGGAGCAAGAAAATTTGGATTTTTAAGTTTGTCTCCTTTGGGTTGCCTACCAGCCCTTAGAGCTGCAAATCCTGAAGCAAACAAAGGTGGTTGTTTTGAAGCAGCTTCTGCTCTTGCTCTAGCACATAACAATGCTTTGAGTAATATTCTTACAAGCCTCAACCAAGTCCTTAAAGGTGGACCATATGGAGGTATCTACACATGTGGTGGCACAAAGACAATTAAAGAATACAACTTATGTGACAATGTTGAAGAGTTTGTATGGTGGGATTCTTTCCACCCAACAGAGAAGATTCATGAGCAATTTGCAAAGGCTTTATGGAATGGATCACCTTCCATAGTTGGACCATACAATTTAGAAAGGCTATTTAACAATGACATCAAACTCATGACTATAGCTGATGTAGTTGATGTCCCAGAAATTGAGCATGGATTCTGA
Protein sequence

>Msa0247020

NQSLNFLCCFIVYIVLIQFIASLSLITSQSHKNEIANGITNNALFIFGDSTVDSGNNNYIDTIPENKADCKPYGQNGVFDKPSGRFSNGRVITDFIEKLGEEKAKDLISEAVYFISIGSNDYMGGYLNNPKMQESYNPEQYIGMVIGNLTQAIQILYEKGARKFGFLSLSPLGCLPALRAANPEANKGGCFEAASALALAHNNALSNILTSLNQVLKGGPYGGIYTCGGTKTIKEYNLCDNVEEFVWWDSFHPTEKIHEQFAKALWNGSPSIVGPYNLERLFNNDIKLMTIADVVDVPEIEHGF*