AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0247320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247320 MtrunA17_Chr2g0302041 93.301 209 12 1 1 209 1 207 3.42e-140 390
Msa0247320 MtrunA17_Chr4g0069721 58.209 134 48 3 38 168 25 153 7.11e-43 142
Msa0247320 MtrunA17_Chr5g0399851 36.066 122 63 3 57 176 20 128 3.75e-15 69.7
Msa0247320 MtrunA17_Chr2g0301131 32.031 128 64 3 58 168 112 233 6.78e-14 68.6
Msa0247320 MtrunA17_Chr4g0070271 32.231 121 45 3 50 168 91 176 1.07e-12 64.3
Msa0247320 MtrunA17_Chr5g0399881 33.333 114 59 3 58 171 44 140 3.12e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0247320 AT5G20900.1 53.448 116 46 4 58 171 57 166 7.23e-31 112
Msa0247320 AT3G43440.1 38.760 129 53 4 57 183 130 234 1.05e-15 73.6
Msa0247320 AT3G43440.2 37.500 128 54 4 56 181 43 146 1.97e-15 70.9
Msa0247320 AT1G74950.1 37.097 124 49 4 59 168 120 228 4.86e-13 66.2
Msa0247320 AT1G19180.2 35.043 117 62 3 54 168 56 160 1.45e-12 63.9
Msa0247320 AT1G19180.1 34.746 118 61 4 54 168 122 226 3.20e-12 64.3
Msa0247320 AT1G19180.3 34.746 118 61 4 54 168 122 226 3.20e-12 64.3

Find 64 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAGTATCTTTCCATTCT+TGG 0.165134 2_2:+29346478 None:intergenic
TGAAAGAAGAGAATTATTTA+AGG 0.202725 2_2:+29350140 None:intergenic
CAGCTTACCATCTTCTATAA+TGG 0.263317 2_2:-29347620 Msa0247320:CDS
TAATTCTCTTCTTTCAGTTT+TGG 0.266561 2_2:-29350133 Msa0247320:five_prime_UTR
TTCCTTCACCTGTTCCTTCA+AGG 0.273672 2_2:-29347370 Msa0247320:CDS
GGTTAAGCTTGATGTTATAT+TGG 0.307521 2_2:-29346504 Msa0247320:three_prime_UTR
TCCTTCATTGAAACCGAATC+TGG 0.319218 2_2:+29346919 None:intergenic
TTATACTTTATGTGTTCTTT+CGG 0.344404 2_2:-29346837 Msa0247320:three_prime_UTR
TCTAGTATCTTTCCATTCTT+GGG 0.357304 2_2:+29346479 None:intergenic
TTGAGCAGAATTCTAGTGTT+TGG 0.362255 2_2:-29346860 Msa0247320:exon
TGCTGACAATTCTCCAGATT+CGG 0.364266 2_2:-29346932 Msa0247320:CDS
AAAGTAACCGGCAATGATTA+TGG 0.369608 2_2:-29350260 Msa0247320:five_prime_UTR
TTTAGGTCAATGCCAGCATT+TGG 0.374746 2_2:-29347671 Msa0247320:intron
TCTTCTCCTATTGCTGTTGA+TGG 0.379691 2_2:-29350032 Msa0247320:CDS
AGTGAATGTCTGCGTGCTAT+TGG 0.383673 2_2:+29347137 None:intergenic
TGAGTTGTATTAGCAATCAT+TGG 0.398568 2_2:+29347641 None:intergenic
AGCTTACCATCTTCTATAAT+GGG 0.398580 2_2:-29347619 Msa0247320:CDS
GAAAGAAGAGAATTATTTAA+GGG 0.411915 2_2:+29350141 None:intergenic
TCCAGATTCGGTTTCAATGA+AGG 0.413460 2_2:-29346920 Msa0247320:CDS
CTGCAGATAGAACTCTTTGC+AGG 0.418330 2_2:+29347290 None:intergenic
CACATGTTGCTTAAAGTAAC+CGG 0.424321 2_2:-29350272 Msa0247320:five_prime_UTR
ATTGGATTCGTTCCAAATGC+TGG 0.440801 2_2:+29347659 None:intergenic
CTAGCACCATCAACAGCAAT+AGG 0.441302 2_2:+29350026 None:intergenic
GCTAGCTCCAACATGGTTGA+GGG 0.442261 2_2:-29350008 Msa0247320:CDS
AATGGATGGTGTTACTGTTA+AGG 0.443386 2_2:-29350084 Msa0247320:CDS
ACAGGTGAAGGAACAGGAAC+GGG 0.448799 2_2:+29347380 None:intergenic
TTCTTCAGAAGCGGCGAGAC+AGG 0.453708 2_2:-29347106 Msa0247320:intron
ACTTCGAAGTTGCATCTCTC+TGG 0.455499 2_2:-29346704 Msa0247320:three_prime_UTR
GTTATATTGGATCCCAAGAA+TGG 0.460430 2_2:-29346491 Msa0247320:three_prime_UTR
GTTGGCTGAAATTCTTCCAT+TGG 0.460471 2_2:+29346895 None:intergenic
TGACCAACCATAATCATTGC+CGG 0.463542 2_2:+29350253 None:intergenic
CTTCTTCGTTTCACTAGACT+TGG 0.471119 2_2:+29347418 None:intergenic
TGCTAGCTCCAACATGGTTG+AGG 0.475742 2_2:-29350009 Msa0247320:CDS
TGATGGAATTCCTGCAGAAA+AGG 0.477638 2_2:-29347582 Msa0247320:intron
GGGAGTATTTGTATCTATGA+TGG 0.478209 2_2:-29347599 Msa0247320:CDS
GCAGTTCCATGTGGCGAAGA+AGG 0.484372 2_2:+29347347 None:intergenic
CTGCAAAGAGTTCTATCTGC+AGG 0.484476 2_2:-29347289 Msa0247320:CDS
TCTAGAACAGTAAACTTCTC+TGG 0.487430 2_2:+29350056 None:intergenic
GAAGGAACAGGTGAAGGAAC+AGG 0.487448 2_2:+29347374 None:intergenic
TGTGGCGAAGAAGGCCTTGA+AGG 0.491077 2_2:+29347356 None:intergenic
ATAGAACTCTTTGCAGGAAA+GGG 0.495560 2_2:+29347296 None:intergenic
TTCAAATGTTTCTTCAGAAG+CGG 0.502165 2_2:-29347115 Msa0247320:CDS
AATACTCCCATTATAGAAGA+TGG 0.513403 2_2:+29347613 None:intergenic
CTTTATGTGTTCTTTCGGCA+AGG 0.514767 2_2:-29346832 Msa0247320:three_prime_UTR
TTTATGTGTTCTTTCGGCAA+GGG 0.524003 2_2:-29346831 Msa0247320:three_prime_UTR
GAAGAAGGCCTTGAAGGAAC+AGG 0.524051 2_2:+29347362 None:intergenic
GCTCAAGATGCAGATATAGT+TGG 0.526009 2_2:+29346877 None:intergenic
TCAAGGCCTTCTTCGCCACA+TGG 0.540794 2_2:-29347353 Msa0247320:CDS
AGTTCTATCTGCAGGCTGCA+AGG 0.555717 2_2:-29347281 Msa0247320:intron
GATAGAACTCTTTGCAGGAA+AGG 0.557564 2_2:+29347295 None:intergenic
TGATAATATCGCTTCACCAC+AGG 0.571663 2_2:-29347324 Msa0247320:CDS
GGCCTTGAAGGAACAGGTGA+AGG 0.574210 2_2:+29347368 None:intergenic
CATTGACCCCTCAACCATGT+TGG 0.579797 2_2:+29350001 None:intergenic
GGTTTCAATGAAGGAACCAA+TGG 0.581761 2_2:-29346911 Msa0247320:CDS
AACAGGTGAAGGAACAGGAA+CGG 0.583312 2_2:+29347379 None:intergenic
AACAAAGGTAGCTGACAACA+TGG 0.591184 2_2:-29346968 Msa0247320:CDS
TAACCGGCAATGATTATGGT+TGG 0.602717 2_2:-29350256 Msa0247320:five_prime_UTR
TGATGGTGCTAGCTCCAACA+TGG 0.603125 2_2:-29350015 Msa0247320:CDS
GACAACATGGAGAACAACTT+CGG 0.616517 2_2:-29346955 Msa0247320:CDS
GAACTCTTTGCAGGAAAGGG+AGG 0.634573 2_2:+29347299 None:intergenic
GCAGGAAAGGGAGGTGCCTG+TGG 0.640323 2_2:+29347308 None:intergenic
TCTACAAACAGTAAAATACA+AGG 0.647478 2_2:+29346629 None:intergenic
CTAGCTCCAACATGGTTGAG+GGG 0.656060 2_2:-29350007 Msa0247320:CDS
ATATTATCAGCAGTTCCATG+TGG 0.739266 2_2:+29347338 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAACTTTGTAAAATTTT+TGG - chr2_2:29346605-29346624 Msa0247320:three_prime_UTR 10.0%
!!! ATTTTAACTCAAAAAACTAA+AGG + chr2_2:29347118-29347137 None:intergenic 15.0%
!! ATCTTAGTTATTTAAAAAGT+TGG + chr2_2:29348347-29348366 None:intergenic 15.0%
!! CATTACAATATTCCAAAAAA+AGG - chr2_2:29346548-29346567 Msa0247320:three_prime_UTR 20.0%
!! GAAAGAAGAGAATTATTTAA+GGG + chr2_2:29346642-29346661 None:intergenic 20.0%
!! TGAAAGAAGAGAATTATTTA+AGG + chr2_2:29346643-29346662 None:intergenic 20.0%
!! CAAAACAGAAACAAAAAAAA+GGG + chr2_2:29346923-29346942 None:intergenic 20.0%
!! TCAAAACAGAAACAAAAAAA+AGG + chr2_2:29346924-29346943 None:intergenic 20.0%
!!! TAGCTTGGTTATTTTAATTT+TGG - chr2_2:29346959-29346978 Msa0247320:CDS 20.0%
!!! ATACAAATTGATACCATTTT+AGG + chr2_2:29347070-29347089 None:intergenic 20.0%
!!! CTACGAAAAAACAAAATTTT+CGG - chr2_2:29347201-29347220 Msa0247320:intron 20.0%
!! TTCTATTATGCAATGAATTA+GGG + chr2_2:29347482-29347501 None:intergenic 20.0%
!! TAATTCATTGCATAATAGAA+CGG - chr2_2:29347482-29347501 Msa0247320:intron 20.0%
!! ATATGAAAATCGAAAAGATT+AGG + chr2_2:29347848-29347867 None:intergenic 20.0%
!! ATACACTGATACATATATTT+AGG - chr2_2:29348851-29348870 Msa0247320:intron 20.0%
!!! ACTGTATTATTTTCTCTTTT+AGG - chr2_2:29349092-29349111 Msa0247320:intron 20.0%
!! ATAATTTATGTTTGTACTAC+AGG - chr2_2:29349754-29349773 Msa0247320:intron 20.0%
!! TTATACTTTATGTGTTCTTT+CGG - chr2_2:29349943-29349962 Msa0247320:intron 20.0%
!!! TTTTGTAAGAATGAAAAAAC+AGG + chr2_2:29350243-29350262 None:intergenic 20.0%
!!! CAAAGTTTTTTGAGTTTCAA+TGG + chr2_2:29346596-29346615 None:intergenic 25.0%
!! TAATTCTCTTCTTTCAGTTT+TGG - chr2_2:29346647-29346666 Msa0247320:three_prime_UTR 25.0%
! TAATCAAAAACATGCTTTCT+TGG + chr2_2:29346845-29346864 None:intergenic 25.0%
!! TTCCAATTTTGTACCTAAAA+TGG - chr2_2:29347054-29347073 Msa0247320:intron 25.0%
!!! TACCATTTTAGGTACAAAAT+TGG + chr2_2:29347059-29347078 None:intergenic 25.0%
! TTATGAGAAACCATTAAACA+AGG + chr2_2:29347247-29347266 None:intergenic 25.0%
! ACAAACAATTGATGTTTGTA+TGG - chr2_2:29347349-29347368 Msa0247320:CDS 25.0%
! GTTCTATTATGCAATGAATT+AGG + chr2_2:29347483-29347502 None:intergenic 25.0%
! AAGAACTCAACGATTAATTA+TGG + chr2_2:29347556-29347575 None:intergenic 25.0%
! TGTTCTTATCTTAGTAGTTA+AGG - chr2_2:29347651-29347670 Msa0247320:CDS 25.0%
! GTTCTTATCTTAGTAGTTAA+GGG - chr2_2:29347652-29347671 Msa0247320:CDS 25.0%
! TAGTTAAGGGATAGAATAAA+GGG - chr2_2:29347665-29347684 Msa0247320:CDS 25.0%
!! TTTCTTTTCAATTCAACTCT+TGG - chr2_2:29347878-29347897 Msa0247320:intron 25.0%
!!! TGTCTTCATTGATTTAGAAA+AGG + chr2_2:29347924-29347943 None:intergenic 25.0%
!!! ATGAAAAAGTATTAAGTCCA+AGG + chr2_2:29348320-29348339 None:intergenic 25.0%
!!! AGATATGTCATTGTTTTTCA+TGG - chr2_2:29348502-29348521 Msa0247320:intron 25.0%
! ACATGAATTTCATCAACTTA+TGG + chr2_2:29348585-29348604 None:intergenic 25.0%
! AATGGAATAAGTCATTTGAA+TGG + chr2_2:29348650-29348669 None:intergenic 25.0%
! AGTGTATATGCTACATTAAT+GGG + chr2_2:29348838-29348857 None:intergenic 25.0%
! TTAACTCTCTATATACTAAC+AGG - chr2_2:29349306-29349325 Msa0247320:intron 25.0%
!! CATACACAAAATTTGAAACA+TGG + chr2_2:29349713-29349732 None:intergenic 25.0%
! TCTACAAACAGTAAAATACA+AGG + chr2_2:29350154-29350173 None:intergenic 25.0%
!! ATTTTTGAACATCAACACAA+TGG + chr2_2:29350188-29350207 None:intergenic 25.0%
! TTACAAAAACAGTGATTCAT+AGG - chr2_2:29350255-29350274 Msa0247320:five_prime_UTR 25.0%
!!! GCTGTTTTGATTCCTTTTTT+TGG + chr2_2:29346563-29346582 None:intergenic 30.0%
!! AGTTTTGGTGATCTATTTGT+AGG - chr2_2:29346662-29346681 Msa0247320:three_prime_UTR 30.0%
!! GTTTTGGTGATCTATTTGTA+GGG - chr2_2:29346663-29346682 Msa0247320:three_prime_UTR 30.0%
TTGTAGGGTTCTTCAAAAAA+TGG - chr2_2:29346678-29346697 Msa0247320:three_prime_UTR 30.0%
AACAAAAAAAAGGGTCTTGA+AGG + chr2_2:29346914-29346933 None:intergenic 30.0%
TGAATAATTACTCACTAGCT+TGG - chr2_2:29346944-29346963 Msa0247320:CDS 30.0%
AAAATTGTTCTGATGAGTTG+AGG - chr2_2:29347133-29347152 Msa0247320:CDS 30.0%
! AAATTGTTCTGATGAGTTGA+GGG - chr2_2:29347134-29347153 Msa0247320:CDS 30.0%
!!! TGTTTTTTCGTAGAGTCAAA+TGG + chr2_2:29347194-29347213 None:intergenic 30.0%
CGGTTAAATTGTTTATCCTA+TGG - chr2_2:29347221-29347240 Msa0247320:intron 30.0%
! CAAACATCAATTGTTTGTTG+AGG + chr2_2:29347347-29347366 None:intergenic 30.0%
GAAAGGTTACAATAGAAATC+TGG + chr2_2:29347602-29347621 None:intergenic 30.0%
GTAGTTAAGGGATAGAATAA+AGG - chr2_2:29347664-29347683 Msa0247320:CDS 30.0%
! TACATCTTTTCATTGAACCT+TGG - chr2_2:29347969-29347988 Msa0247320:intron 30.0%
! GTCAGATATAATAGGTGTTT+TGG + chr2_2:29348150-29348169 None:intergenic 30.0%
TGGTCTATGTCAGATATAAT+AGG + chr2_2:29348158-29348177 None:intergenic 30.0%
AACAATCCACCATATAAACA+TGG + chr2_2:29348186-29348205 None:intergenic 30.0%
!! ATGTTTATATGGTGGATTGT+TGG - chr2_2:29348185-29348204 Msa0247320:intron 30.0%
! ATAGATAACAGGGGATATTT+TGG - chr2_2:29348235-29348254 Msa0247320:intron 30.0%
!!! TTTTGGTTTTTTGGCTTTTC+TGG - chr2_2:29348252-29348271 Msa0247320:intron 30.0%
! TTTTTCTGAATTCTGAACCT+TGG - chr2_2:29348300-29348319 Msa0247320:intron 30.0%
! TGATTGAACAACAAAAGCAT+AGG - chr2_2:29348401-29348420 Msa0247320:intron 30.0%
TCTGTTCTGTATGAAATCAT+TGG - chr2_2:29348464-29348483 Msa0247320:intron 30.0%
TGTGTATAACCAAATTGAAC+AGG - chr2_2:29348602-29348621 Msa0247320:intron 30.0%
GTGTATAACCAAATTGAACA+GGG - chr2_2:29348603-29348622 Msa0247320:intron 30.0%
TCTCGCTGAAAAATATGTAA+TGG + chr2_2:29348668-29348687 None:intergenic 30.0%
!! TATTTTTCAGCGAGATTTTG+AGG - chr2_2:29348673-29348692 Msa0247320:intron 30.0%
! GTTGCAGATTTTTATTGAAC+TGG + chr2_2:29348812-29348831 None:intergenic 30.0%
GTGTATATGCTACATTAATG+GGG + chr2_2:29348837-29348856 None:intergenic 30.0%
CAGTGTATATGCTACATTAA+TGG + chr2_2:29348839-29348858 None:intergenic 30.0%
TTCAAAGAACTTAGTCATCA+AGG - chr2_2:29348966-29348985 Msa0247320:intron 30.0%
! GCTTTTTGTGTTTGTTTGAA+TGG - chr2_2:29348998-29349017 Msa0247320:intron 30.0%
TGAGTTGTATTAGCAATCAT+TGG + chr2_2:29349142-29349161 None:intergenic 30.0%
AGCTTACCATCTTCTATAAT+GGG - chr2_2:29349161-29349180 Msa0247320:intron 30.0%
AATACTCCCATTATAGAAGA+TGG + chr2_2:29349170-29349189 None:intergenic 30.0%
CACACGAGACATATAAATTA+TGG + chr2_2:29349594-29349613 None:intergenic 30.0%
CATAATTTATATGTCTCGTG+TGG - chr2_2:29349592-29349611 Msa0247320:intron 30.0%
TTCAAATGTTTCTTCAGAAG+CGG - chr2_2:29349665-29349684 Msa0247320:intron 30.0%
TTTATGTTTGTACTACAGGT+TGG - chr2_2:29349758-29349777 Msa0247320:intron 30.0%
TTATGTTTGTACTACAGGTT+GGG - chr2_2:29349759-29349778 Msa0247320:intron 30.0%
GTAAGAATGAAAAAACAGGA+AGG + chr2_2:29350239-29350258 None:intergenic 30.0%
GGTTAAGCTTGATGTTATAT+TGG - chr2_2:29350276-29350295 Msa0247320:five_prime_UTR 30.0%
! CACATGTTGCTTAAAGTAAC+CGG - chr2_2:29346508-29346527 Msa0247320:three_prime_UTR 35.0%
AAAGTAACCGGCAATGATTA+TGG - chr2_2:29346520-29346539 Msa0247320:three_prime_UTR 35.0%
AGGGTTCTTCAAAAAATGGA+TGG - chr2_2:29346682-29346701 Msa0247320:three_prime_UTR 35.0%
!! AATGGATGGTGTTACTGTTA+AGG - chr2_2:29346696-29346715 Msa0247320:three_prime_UTR 35.0%
! TCTAGAACAGTAAACTTCTC+TGG + chr2_2:29346727-29346746 None:intergenic 35.0%
!! TATCCTATGGCCTTGTTTAA+TGG - chr2_2:29347234-29347253 Msa0247320:intron 35.0%
AAACCATTAAACAAGGCCAT+AGG + chr2_2:29347240-29347259 None:intergenic 35.0%
TGTTGAGTTTGTGTTGAGTA+TGG - chr2_2:29347382-29347401 Msa0247320:CDS 35.0%
AAATACTACGAGCCACTTAT+CGG + chr2_2:29347418-29347437 None:intergenic 35.0%
CAACGATTAATTATGGAGCT+CGG + chr2_2:29347549-29347568 None:intergenic 35.0%
CATCTGATTGTACAGTAGAA+AGG + chr2_2:29347619-29347638 None:intergenic 35.0%
AGAGTAGTTAGTTAGCTGTT+TGG - chr2_2:29347720-29347739 Msa0247320:intron 35.0%
TAGTTAGTTAGCTGTTTGGT+TGG - chr2_2:29347724-29347743 Msa0247320:intron 35.0%
AGTTAGTTAGCTGTTTGGTT+GGG - chr2_2:29347725-29347744 Msa0247320:intron 35.0%
AGTCATGACTCTATTGTGAA+AGG - chr2_2:29347801-29347820 Msa0247320:intron 35.0%
GTCATGACTCTATTGTGAAA+GGG - chr2_2:29347802-29347821 Msa0247320:intron 35.0%
ATTAGGAGTCTCTTCTTCAA+GGG + chr2_2:29347831-29347850 None:intergenic 35.0%
AAAAGGCTTGTGACAATGTT+AGG + chr2_2:29347907-29347926 None:intergenic 35.0%
TAATTTCGCTTATGACAGAG+AGG + chr2_2:29348015-29348034 None:intergenic 35.0%
!!! ATAATAGGTGTTTTGGCCTA+TGG + chr2_2:29348143-29348162 None:intergenic 35.0%
ACCACATCACCATGTTTATA+TGG - chr2_2:29348174-29348193 Msa0247320:intron 35.0%
! ACCATATAAACATGGTGATG+TGG + chr2_2:29348178-29348197 None:intergenic 35.0%
ACATCACCATGTTTATATGG+TGG - chr2_2:29348177-29348196 Msa0247320:intron 35.0%
!!! CAGGGGATATTTTGGTTTTT+TGG - chr2_2:29348243-29348262 Msa0247320:intron 35.0%
TGAAACACTGGAAATCTCAA+TGG + chr2_2:29348430-29348449 None:intergenic 35.0%
! ACTTTTGTCCCTGTTCAATT+TGG + chr2_2:29348614-29348633 None:intergenic 35.0%
AGGCTTCTTCATGCATATTA+TGG - chr2_2:29348693-29348712 Msa0247320:intron 35.0%
!! TTTCCACGTTTGTGTTTTGA+AGG - chr2_2:29348785-29348804 Msa0247320:intron 35.0%
!! TTCCACGTTTGTGTTTTGAA+GGG - chr2_2:29348786-29348805 Msa0247320:intron 35.0%
CAGCTTACCATCTTCTATAA+TGG - chr2_2:29349160-29349179 Msa0247320:intron 35.0%
!! GGGAGTATTTGTATCTATGA+TGG - chr2_2:29349181-29349200 Msa0247320:intron 35.0%
ATTTCGATTGTGTCGTAACA+TGG + chr2_2:29349281-29349300 None:intergenic 35.0%
ATATTATCAGCAGTTCCATG+TGG + chr2_2:29349445-29349464 None:intergenic 35.0%
ATAGAACTCTTTGCAGGAAA+GGG + chr2_2:29349487-29349506 None:intergenic 35.0%
TAGTCGAAGATATGACTAGT+AGG + chr2_2:29349535-29349554 None:intergenic 35.0%
! CACAAAATTTGAAACATGGC+AGG + chr2_2:29349709-29349728 None:intergenic 35.0%
!!! TTGTCAGCTACCTTTGTTTT+TGG + chr2_2:29349810-29349829 None:intergenic 35.0%
TTGAGCAGAATTCTAGTGTT+TGG - chr2_2:29349920-29349939 Msa0247320:intron 35.0%
TTTATGTGTTCTTTCGGCAA+GGG - chr2_2:29349949-29349968 Msa0247320:intron 35.0%
GTTATATTGGATCCCAAGAA+TGG - chr2_2:29350289-29350308 Msa0247320:five_prime_UTR 35.0%
TAACCGGCAATGATTATGGT+TGG - chr2_2:29346524-29346543 Msa0247320:three_prime_UTR 40.0%
TGACCAACCATAATCATTGC+CGG + chr2_2:29346530-29346549 None:intergenic 40.0%
! TCTTCTCCTATTGCTGTTGA+TGG - chr2_2:29346748-29346767 Msa0247320:three_prime_UTR 40.0%
! GTTTGTTGAGGAGAAAAGCA+GGG + chr2_2:29347335-29347354 None:intergenic 40.0%
! TGTTTGTTGAGGAGAAAAGC+AGG + chr2_2:29347336-29347355 None:intergenic 40.0%
GTTAGAGATGTACACGCAAT+TGG - chr2_2:29347523-29347542 Msa0247320:intron 40.0%
GATTAGGAGTCTCTTCTTCA+AGG + chr2_2:29347832-29347851 None:intergenic 40.0%
ACCTTGGTATGTATCCTTCA+CGG - chr2_2:29347985-29348004 Msa0247320:intron 40.0%
TATCTGCAGATGGCAGATTA+TGG + chr2_2:29348218-29348237 None:intergenic 40.0%
CCATCTGCAGATAGATAACA+GGG - chr2_2:29348225-29348244 Msa0247320:intron 40.0%
CATCTGCAGATAGATAACAG+GGG - chr2_2:29348226-29348245 Msa0247320:intron 40.0%
TCATACAGAACAGAACAGAG+AGG + chr2_2:29348458-29348477 None:intergenic 40.0%
GCAGTCAAAAACTGTTGATG+CGG - chr2_2:29348722-29348741 Msa0247320:intron 40.0%
ATGCTACACAACCGTGATTA+AGG + chr2_2:29348748-29348767 None:intergenic 40.0%
! CTCCTTGTACTGAGTTTTCT+AGG - chr2_2:29348899-29348918 Msa0247320:intron 40.0%
AGCCTAGAAAACTCAGTACA+AGG + chr2_2:29348904-29348923 None:intergenic 40.0%
TTTAGGTCAATGCCAGCATT+TGG - chr2_2:29349109-29349128 Msa0247320:intron 40.0%
ATTGGATTCGTTCCAAATGC+TGG + chr2_2:29349124-29349143 None:intergenic 40.0%
TGATGGAATTCCTGCAGAAA+AGG - chr2_2:29349198-29349217 Msa0247320:intron 40.0%
CTTCTTCGTTTCACTAGACT+TGG + chr2_2:29349365-29349384 None:intergenic 40.0%
TGATAATATCGCTTCACCAC+AGG - chr2_2:29349456-29349475 Msa0247320:intron 40.0%
GATAGAACTCTTTGCAGGAA+AGG + chr2_2:29349488-29349507 None:intergenic 40.0%
AACAAAGGTAGCTGACAACA+TGG - chr2_2:29349812-29349831 Msa0247320:intron 40.0%
GACAACATGGAGAACAACTT+CGG - chr2_2:29349825-29349844 Msa0247320:intron 40.0%
TGCTGACAATTCTCCAGATT+CGG - chr2_2:29349848-29349867 Msa0247320:intron 40.0%
! TCCAGATTCGGTTTCAATGA+AGG - chr2_2:29349860-29349879 Msa0247320:intron 40.0%
TCCTTCATTGAAACCGAATC+TGG + chr2_2:29349864-29349883 None:intergenic 40.0%
GGTTTCAATGAAGGAACCAA+TGG - chr2_2:29349869-29349888 Msa0247320:intron 40.0%
GTTGGCTGAAATTCTTCCAT+TGG + chr2_2:29349888-29349907 None:intergenic 40.0%
GCTCAAGATGCAGATATAGT+TGG + chr2_2:29349906-29349925 None:intergenic 40.0%
CTTTATGTGTTCTTTCGGCA+AGG - chr2_2:29349948-29349967 Msa0247320:intron 40.0%
CTAGCACCATCAACAGCAAT+AGG + chr2_2:29346757-29346776 None:intergenic 45.0%
ACCGTGAAGGATACATACCA+AGG + chr2_2:29347989-29348008 None:intergenic 45.0%
GCCATCTGCAGATAGATAAC+AGG - chr2_2:29348224-29348243 Msa0247320:intron 45.0%
CCCTGTTATCTATCTGCAGA+TGG + chr2_2:29348228-29348247 None:intergenic 45.0%
! AGAGAGGTATGCTGAAACAC+TGG + chr2_2:29348442-29348461 None:intergenic 45.0%
TGTTGATGCGGCCTTAATCA+CGG - chr2_2:29348734-29348753 Msa0247320:intron 45.0%
AACAGGTGAAGGAACAGGAA+CGG + chr2_2:29349404-29349423 None:intergenic 45.0%
TTCCTTCACCTGTTCCTTCA+AGG - chr2_2:29349410-29349429 Msa0247320:intron 45.0%
CTGCAGATAGAACTCTTTGC+AGG + chr2_2:29349493-29349512 None:intergenic 45.0%
CTGCAAAGAGTTCTATCTGC+AGG - chr2_2:29349491-29349510 Msa0247320:intron 45.0%
AGTGAATGTCTGCGTGCTAT+TGG + chr2_2:29349646-29349665 None:intergenic 45.0%
!!! GTTTTTGGCGAGGAAGGATA+GGG + chr2_2:29349795-29349814 None:intergenic 45.0%
!!! TGTTTTTGGCGAGGAAGGAT+AGG + chr2_2:29349796-29349815 None:intergenic 45.0%
TCCTTCCTCGCCAAAAACAA+AGG - chr2_2:29349797-29349816 Msa0247320:intron 45.0%
!!! ACCTTTGTTTTTGGCGAGGA+AGG + chr2_2:29349801-29349820 None:intergenic 45.0%
!!! AGCTACCTTTGTTTTTGGCG+AGG + chr2_2:29349805-29349824 None:intergenic 45.0%
ACTTCGAAGTTGCATCTCTC+TGG - chr2_2:29350076-29350095 Msa0247320:CDS 45.0%
! TGATGGTGCTAGCTCCAACA+TGG - chr2_2:29346765-29346784 Msa0247320:three_prime_UTR 50.0%
TGCTAGCTCCAACATGGTTG+AGG - chr2_2:29346771-29346790 Msa0247320:three_prime_UTR 50.0%
GCTAGCTCCAACATGGTTGA+GGG - chr2_2:29346772-29346791 Msa0247320:three_prime_UTR 50.0%
CTAGCTCCAACATGGTTGAG+GGG - chr2_2:29346773-29346792 Msa0247320:three_prime_UTR 50.0%
CATTGACCCCTCAACCATGT+TGG + chr2_2:29346782-29346801 None:intergenic 50.0%
TGTTGAGGAGAAAAGCAGGG+AGG + chr2_2:29347332-29347351 None:intergenic 50.0%
GGCTCATTGTCACCGATAAG+TGG - chr2_2:29347403-29347422 Msa0247320:CDS 50.0%
CTGCAGATGGCAGATTATGG+TGG + chr2_2:29348215-29348234 None:intergenic 50.0%
GGCCCTTCAAAACACAAACG+TGG + chr2_2:29348791-29348810 None:intergenic 50.0%
ACAGGTGAAGGAACAGGAAC+GGG + chr2_2:29349403-29349422 None:intergenic 50.0%
GAAGGAACAGGTGAAGGAAC+AGG + chr2_2:29349409-29349428 None:intergenic 50.0%
GAAGAAGGCCTTGAAGGAAC+AGG + chr2_2:29349421-29349440 None:intergenic 50.0%
GAACTCTTTGCAGGAAAGGG+AGG + chr2_2:29349484-29349503 None:intergenic 50.0%
AGTTCTATCTGCAGGCTGCA+AGG - chr2_2:29349499-29349518 Msa0247320:intron 50.0%
GACAGAGAGGTCAACCGTGA+AGG + chr2_2:29348002-29348021 None:intergenic 55.0%
TTGGCCTATGGCAACTGCCA+CGG + chr2_2:29348131-29348150 None:intergenic 55.0%
! CGTAGGGCTACCTTTTCTGC+AGG + chr2_2:29349211-29349230 None:intergenic 55.0%
CAGTGTAGCGTAGCTGCGTA+GGG + chr2_2:29349227-29349246 None:intergenic 55.0%
TCAGTGTAGCGTAGCTGCGT+AGG + chr2_2:29349228-29349247 None:intergenic 55.0%
GGCCTTGAAGGAACAGGTGA+AGG + chr2_2:29349415-29349434 None:intergenic 55.0%
TGTGGCGAAGAAGGCCTTGA+AGG + chr2_2:29349427-29349446 None:intergenic 55.0%
TCAAGGCCTTCTTCGCCACA+TGG - chr2_2:29349427-29349446 Msa0247320:intron 55.0%
GCAGTTCCATGTGGCGAAGA+AGG + chr2_2:29349436-29349455 None:intergenic 55.0%
TTCTTCAGAAGCGGCGAGAC+AGG - chr2_2:29349674-29349693 Msa0247320:intron 55.0%
TGAGCACCACCAATGTGCCG+TGG - chr2_2:29348111-29348130 Msa0247320:intron 60.0%
CAACTGCCACGGCACATTGG+TGG + chr2_2:29348120-29348139 None:intergenic 60.0%
TGGCAACTGCCACGGCACAT+TGG + chr2_2:29348123-29348142 None:intergenic 60.0%
TGTGCCGTGGCAGTTGCCAT+AGG - chr2_2:29348124-29348143 Msa0247320:intron 60.0%
!! GCAGGAAAGGGAGGTGCCTG+TGG + chr2_2:29349475-29349494 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 29346482 29350320 29346482 ID=Msa0247320;Name=Msa0247320
chr2_2 mRNA 29346482 29350320 29346482 ID=Msa0247320-mRNA-1;Parent=Msa0247320;Name=Msa0247320-mRNA-1;_AED=0.10;_eAED=0.10;_QI=215|1|1|1|1|1|5|397|209
chr2_2 exon 29346482 29347026 29346482 ID=Msa0247320-mRNA-1:exon:10456;Parent=Msa0247320-mRNA-1
chr2_2 exon 29347107 29347164 29347107 ID=Msa0247320-mRNA-1:exon:10455;Parent=Msa0247320-mRNA-1
chr2_2 exon 29347282 29347474 29347282 ID=Msa0247320-mRNA-1:exon:10454;Parent=Msa0247320-mRNA-1
chr2_2 exon 29347583 29347688 29347583 ID=Msa0247320-mRNA-1:exon:10453;Parent=Msa0247320-mRNA-1
chr2_2 exon 29349981 29350320 29349981 ID=Msa0247320-mRNA-1:exon:10452;Parent=Msa0247320-mRNA-1
chr2_2 five_prime_UTR 29350106 29350320 29350106 ID=Msa0247320-mRNA-1:five_prime_utr;Parent=Msa0247320-mRNA-1
chr2_2 CDS 29349981 29350105 29349981 ID=Msa0247320-mRNA-1:cds;Parent=Msa0247320-mRNA-1
chr2_2 CDS 29347583 29347688 29347583 ID=Msa0247320-mRNA-1:cds;Parent=Msa0247320-mRNA-1
chr2_2 CDS 29347282 29347474 29347282 ID=Msa0247320-mRNA-1:cds;Parent=Msa0247320-mRNA-1
chr2_2 CDS 29347107 29347164 29347107 ID=Msa0247320-mRNA-1:cds;Parent=Msa0247320-mRNA-1
chr2_2 CDS 29346879 29347026 29346879 ID=Msa0247320-mRNA-1:cds;Parent=Msa0247320-mRNA-1
chr2_2 three_prime_UTR 29346482 29346878 29346482 ID=Msa0247320-mRNA-1:three_prime_utr;Parent=Msa0247320-mRNA-1
Gene Sequence

>Msa0247320

ATGGATGGTGTTACTGTTAAGGTTGAGCCAGAGAAGTTTACTGTTCTAGAATCTTCTCCTATTGCTGTTGATGGTGCTAGCTCCAACATGGTTGAGGGGTCAATGACGAATTCTTCAGCAAACAAGTCAATGCCAGCATTTGGAACGAATCCAATGATTGCTAATACAACTCAGCTTACCATCTTCTATAATGGGAGTATTTGTATCTATGATGGAATTCCTGCAGAAAAGGTGCAAGAAATAATGCGCATTGCTGCTGCTGCTGCCAAGTCTAGTGAAACGAAGAAGATTGTGAAACAATCTCCCGTTCCTGTTCCTTCACCTGTTCCTTCAAGGCCTTCTTCGCCACATGGAACTGCTGATAATATCGCTTCACCACAGGCACCTCCCTTTCCTGCAAAGAGTTCTATCTGCAGGCTGCAAGAATTTCCAATAGCACGCAGACATTCACTTCAAATGTTTCTTCAGAAGCGGCGAGACAGGTTGGGTAGTAAAGCTCCCTATCCTTCCTCGCCAAAAACAAAGGTAGCTGACAACATGGAGAACAACTTCGGTGCTGACAATTCTCCAGATTCGGTTTCAATGAAGGAACCAATGGAAGAATTTCAGCCAACTATATCTGCATCTTGA
Protein sequence

>Msa0247320

MDGVTVKVEPEKFTVLESSPIAVDGASSNMVEGSMTNSSANKSMPAFGTNPMIANTTQLTIFYNGSICIYDGIPAEKVQEIMRIAAAAAKSSETKKIVKQSPVPVPSPVPSRPSSPHGTADNIASPQAPPFPAKSSICRLQEFPIARRHSLQMFLQKRRDRLGSKAPYPSSPKTKVADNMENNFGADNSPDSVSMKEPMEEFQPTISAS*