AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0251290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251290 MtrunA17_Chr2g0309681 97.954 391 6 2 1 390 1 390 0.0 786
Msa0251290 MtrunA17_Chr8g0356301 77.411 394 73 7 1 389 1 383 0.0 571
Msa0251290 MtrunA17_Chr4g0073991 51.309 191 91 1 200 388 147 337 1.07e-67 218
Msa0251290 MtrunA17_Chr5g0420791 48.438 192 97 1 199 388 110 301 3.25e-67 215
Msa0251290 MtrunA17_Chr4g0001371 49.738 191 94 1 198 386 134 324 1.59e-66 215
Msa0251290 MtrunA17_Chr8g0366071 48.205 195 96 2 199 388 114 308 5.60e-66 212
Msa0251290 MtrunA17_Chr3g0141411 45.701 221 116 3 169 386 97 316 2.63e-64 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251290 AT3G12710.1 70.758 277 65 3 115 388 46 309 2.87e-138 397
Msa0251290 AT5G44680.1 51.523 394 146 8 1 390 1 353 5.08e-135 390
Msa0251290 AT1G75090.1 53.191 188 86 1 201 386 119 306 1.03e-72 230
Msa0251290 AT5G57970.1 50.472 212 97 3 183 386 130 341 1.12e-71 228
Msa0251290 AT5G57970.2 50.472 212 97 3 183 386 130 341 1.12e-71 228
Msa0251290 AT1G13635.2 48.529 204 103 1 187 388 101 304 2.53e-70 223
Msa0251290 AT1G13635.1 48.529 204 103 1 187 388 101 304 2.53e-70 223
Msa0251290 AT1G80850.1 54.301 186 83 1 201 384 134 319 5.86e-70 223
Msa0251290 AT1G15970.1 50.256 195 85 2 201 388 145 334 2.20e-65 212
Msa0251290 AT1G15970.2 50.256 195 85 2 201 388 145 334 2.20e-65 212

Find 109 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAGGACTTTGCATTATT+TGG 0.099995 2_2:-40738143 Msa0251290:three_prime_UTR
TGTTACTAATGGTGTTATTA+AGG 0.140630 2_2:-40738592 Msa0251290:three_prime_UTR
GTTCATTTGACAAATATATT+TGG 0.144605 2_2:-40739085 Msa0251290:CDS
GCACTTGCAATGCACATTAT+TGG 0.261232 2_2:-40738871 Msa0251290:CDS
TTCATTTGACAAATATATTT+GGG 0.263850 2_2:-40739084 Msa0251290:CDS
CATTTGCTTATCAGTCAAAT+TGG 0.271540 2_2:+40740306 None:intergenic
TAACTACCAATTACCTGAAT+TGG 0.274835 2_2:+40741308 None:intergenic
TATCCCACACAGGCTGTTAA+AGG 0.287777 2_2:+40738117 None:intergenic
ATACTTGTTGAAGAGTGTTT+AGG 0.293004 2_2:-40738097 Msa0251290:three_prime_UTR
TAAGAAGTGGTGGGAGTGTT+AGG 0.297982 2_2:-40738261 Msa0251290:three_prime_UTR
GGTAATGGAGAAAGTGATTT+TGG 0.299287 2_2:+40741780 None:intergenic
ATCTCAAATCAATACAAATT+TGG 0.303182 2_2:-40739041 Msa0251290:CDS
TTTCCTCTCTTTATAGCTAA+TGG 0.311064 2_2:+40741678 None:intergenic
CTAGTAGGTGACTTTGGTTT+TGG 0.311738 2_2:+40741708 None:intergenic
AAAATGAAGATTGCTCATTA+TGG 0.312266 2_2:-40741438 Msa0251290:CDS
GAGTTTCTTCTTTCAAGATT+TGG 0.322751 2_2:+40741819 None:intergenic
CATAATGAAAACTCCAACTT+TGG 0.328226 2_2:-40741604 Msa0251290:CDS
GTTACTAATGGTGTTATTAA+GGG 0.331443 2_2:-40738591 Msa0251290:three_prime_UTR
TACTTGTTGAAGAGTGTTTA+GGG 0.336383 2_2:-40738096 Msa0251290:three_prime_UTR
GGGTCAATTAATCTCTTATA+TGG 0.340630 2_2:+40738845 None:intergenic
TTTATGTCTTCAAGTCTTGT+AGG 0.342840 2_2:+40738824 None:intergenic
TGATTAAGTGGTCATTTGTT+AGG 0.343650 2_2:+40738904 None:intergenic
GTTATTAAGGGAAATAGATT+TGG 0.348369 2_2:-40738579 Msa0251290:three_prime_UTR
GAAGCATAGCTGCTGTTAGA+AGG 0.354512 2_2:-40741491 Msa0251290:CDS
TACAGTTACTTCTTTAGCCT+TGG 0.358427 2_2:+40741920 None:intergenic
ATGTCTATTAGTTCAGAATA+TGG 0.363323 2_2:-40740283 Msa0251290:CDS
GAAGTAGTAGTAGTCTTGTT+TGG 0.369631 2_2:+40741753 None:intergenic
AAGAGAGGAAATGACAATAA+TGG 0.373376 2_2:-40741666 Msa0251290:CDS
CATAATCAAACCTGAAATCT+AGG 0.379996 2_2:+40740460 None:intergenic
GCATCTCTTCTCCTCTTCAT+TGG 0.380550 2_2:+40741344 None:intergenic
ACACTCCCACCACTTCTTAA+CGG 0.386475 2_2:+40738265 None:intergenic
AGGGAAATGTTGTGAGGGAT+TGG 0.388202 2_2:+40742045 None:intergenic
CTAACAGCAGCTATGCTTCC+CGG 0.396009 2_2:+40741495 None:intergenic
TGCTTATCATGATGAAGAAT+GGG 0.396248 2_2:-40740995 Msa0251290:CDS
GTCTTGTTTGGAAGTGGTAA+TGG 0.397911 2_2:+40741765 None:intergenic
TTACAGGTTAAGAAAGGCTT+TGG 0.404283 2_2:-40739107 Msa0251290:intron
TTCTTTGATCACTGATTCAC+CGG 0.407327 2_2:-40741514 Msa0251290:CDS
AGAGCACTTGAAGGATCAAT+AGG 0.410745 2_2:+40741381 None:intergenic
TGTTGTGTATAGTATTAGAT+AGG 0.416676 2_2:-40738723 Msa0251290:three_prime_UTR
AAATAATGCAAAGTCCTTGT+AGG 0.428444 2_2:+40738145 None:intergenic
AAGGAAAATAGGGTTTGTTA+AGG 0.429207 2_2:+40738069 None:intergenic
CTAGTACTAGTAGGTGACTT+TGG 0.431001 2_2:+40741702 None:intergenic
TGTGCTGACTATAGTTTACT+TGG 0.436773 2_2:-40738753 Msa0251290:three_prime_UTR
CGATCTAATATCAGATTTAA+CGG 0.441374 2_2:-40738337 Msa0251290:three_prime_UTR
CATTCATTTATGCAGGCAGC+TGG 0.444232 2_2:-40738927 Msa0251290:CDS
TTGCTTATCATGATGAAGAA+TGG 0.451345 2_2:-40740996 Msa0251290:CDS
TCATGATAAGCAATATAGAT+AGG 0.456509 2_2:+40741006 None:intergenic
TTGCATGTTGGTTGAAGTAC+TGG 0.458812 2_2:+40741849 None:intergenic
TTTGAATCAAGAGCACTTGA+AGG 0.459539 2_2:+40741372 None:intergenic
CCACATGTTGCTAGAATCAA+TGG 0.460004 2_2:-40741876 Msa0251290:CDS
GTAGTAGTCTTGTTTGGAAG+TGG 0.468780 2_2:+40741759 None:intergenic
ATGCCATTACAGGTTAAGAA+AGG 0.470141 2_2:-40739113 Msa0251290:intron
ATTGAAATAAGCAAAGTTAG+AGG 0.477981 2_2:-40740259 Msa0251290:CDS
TGAAAAGAGAGAGGAAAATG+AGG 0.479288 2_2:+40742025 None:intergenic
AAGAGAGGGTTTAGATATGT+TGG 0.481696 2_2:-40738963 Msa0251290:CDS
GCTTACTTGTCATCATGAAC+TGG 0.481850 2_2:+40740967 None:intergenic
GCTAATGGTCTAGTACTAGT+AGG 0.483572 2_2:+40741693 None:intergenic
AGCTTCATCACAGCCAATTC+AGG 0.489518 2_2:-40741321 Msa0251290:intron
TTCTTTCAAGATTTGGAACA+TGG 0.493504 2_2:+40741826 None:intergenic
GAAATGTTGTGAGGGATTGG+AGG 0.499447 2_2:+40742048 None:intergenic
TTATGTCTTCAAGTCTTGTA+GGG 0.499649 2_2:+40738825 None:intergenic
ATCCCACACAGGCTGTTAAA+GGG 0.500349 2_2:+40738118 None:intergenic
AGTGGTTCATTCATTTATGC+AGG 0.501189 2_2:-40738934 Msa0251290:CDS
AGAATTTGATGCAGAAATTG+TGG 0.503076 2_2:-40740329 Msa0251290:CDS
AGCAAAGACATGATTAAGAG+AGG 0.510022 2_2:-40738978 Msa0251290:CDS
AAGAAGTGGTGGGAGTGTTA+GGG 0.512402 2_2:-40738260 Msa0251290:three_prime_UTR
AAATGTTGTGAGGGATTGGA+GGG 0.520939 2_2:+40742049 None:intergenic
GAGCTCAAGTTGGATCTGAC+TGG 0.532946 2_2:-40740503 Msa0251290:CDS
GAAAAGAGAGAGGAAAATGA+GGG 0.533265 2_2:+40742026 None:intergenic
GTGCATTAACTGCGTCAAGC+AGG 0.536560 2_2:-40738557 Msa0251290:three_prime_UTR
GTTCCCTTTAACAGCCTGTG+TGG 0.547525 2_2:-40738121 Msa0251290:three_prime_UTR
GAAAATAGGGTTTGTTAAGG+TGG 0.555882 2_2:+40738072 None:intergenic
TTCCCTTTAACAGCCTGTGT+GGG 0.555932 2_2:-40738120 Msa0251290:three_prime_UTR
GGGAATCCGTTAAGAAGTGG+TGG 0.561251 2_2:-40738271 Msa0251290:three_prime_UTR
GCAAAGACATGATTAAGAGA+GGG 0.567917 2_2:-40738977 Msa0251290:CDS
GCATGTGCAAATAGAAGGCA+AGG 0.568414 2_2:-40738217 Msa0251290:three_prime_UTR
CATGTGCATGTGCAAATAGA+AGG 0.568587 2_2:-40738222 Msa0251290:three_prime_UTR
AAGCATAGCTGCTGTTAGAA+GGG 0.572624 2_2:-40741490 Msa0251290:CDS
ATGTGACATTGTGACCTACA+AGG 0.578765 2_2:-40738159 Msa0251290:three_prime_UTR
TCATTTGACAAATATATTTG+GGG 0.579579 2_2:-40739083 Msa0251290:CDS
TTCAAAGACTACCAATGAAG+AGG 0.580952 2_2:-40741355 Msa0251290:CDS
GCCTGTGGCAAGTGATTAAG+TGG 0.581468 2_2:+40738892 None:intergenic
TGCTGTTAGAAGGGAACAAG+TGG 0.590285 2_2:-40741481 Msa0251290:CDS
TCTAGCAACATGTGGTGTTG+TGG 0.592032 2_2:+40741884 None:intergenic
ATAAATGAATGAACCACTGT+TGG 0.599012 2_2:+40738939 None:intergenic
AGATTGCAATGAAAAGAGAG+AGG 0.603429 2_2:+40742016 None:intergenic
AAATGAGGGAAATGTTGTGA+GGG 0.604544 2_2:+40742040 None:intergenic
ACGACATCTCACAAATCAGA+AGG 0.605972 2_2:+40738684 None:intergenic
AAGGCTAAAGAAGTAACTGT+AGG 0.608755 2_2:-40741918 Msa0251290:CDS
ACCACTTAATCACTTGCCAC+AGG 0.609569 2_2:-40738893 Msa0251290:CDS
ACAAAACATGTGTAGCTCCA+AGG 0.617528 2_2:-40741937 Msa0251290:exon
CATTTGACAAATATATTTGG+GGG 0.621393 2_2:-40739082 Msa0251290:CDS
AAATGATCTTGATCTTGCGT+CGG 0.621447 2_2:+40738463 None:intergenic
CAGGGGAATCCGTTAAGAAG+TGG 0.621578 2_2:-40738274 Msa0251290:three_prime_UTR
TAGATATGTTGGTCCAACAG+TGG 0.633858 2_2:-40738952 Msa0251290:CDS
CCATTGATTCTAGCAACATG+TGG 0.642807 2_2:+40741876 None:intergenic
TTTGGAACATGGTTGCATGT+TGG 0.648256 2_2:+40741837 None:intergenic
TGAAAACTCCAACTTTGGAG+AGG 0.649673 2_2:-40741599 Msa0251290:CDS
AAAATGAGGGAAATGTTGTG+AGG 0.657957 2_2:+40742039 None:intergenic
TTGTAGGTCACAATGTCACA+TGG 0.660768 2_2:+40738161 None:intergenic
AGACCATTAGCTATAAAGAG+AGG 0.661843 2_2:-40741681 Msa0251290:CDS
ATGTGCATTGCAAGTGCCTG+TGG 0.666497 2_2:+40738877 None:intergenic
GGAATCCGTTAAGAAGTGGT+GGG 0.668441 2_2:-40738270 Msa0251290:three_prime_UTR
GCTTATCATGATGAAGAATG+GGG 0.675468 2_2:-40740994 Msa0251290:CDS
ATGTTGCTAGAATCAATGGA+AGG 0.678120 2_2:-40741872 Msa0251290:CDS
TCTTTGATCACTGATTCACC+GGG 0.686123 2_2:-40741513 Msa0251290:CDS
TGGCACTTCAACAAGCACAA+AGG 0.702109 2_2:-40741461 Msa0251290:CDS
CATGTGCAAATAGAAGGCAA+GGG 0.704854 2_2:-40738216 Msa0251290:three_prime_UTR
CTTCAACAAGTATCCCACAC+AGG 0.710515 2_2:+40738107 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATTATAATTAGAGT+CGG + chr2_2:40738922-40738941 None:intergenic 10.0%
!!! TTGATAGTTTAATTTATTAT+AGG - chr2_2:40738973-40738992 Msa0251290:CDS 10.0%
!!! TTTTTTTTACCTGTAAAATT+TGG + chr2_2:40739910-40739929 None:intergenic 15.0%
!! TCATAAAAATTATCATCAAA+TGG - chr2_2:40740849-40740868 Msa0251290:intron 15.0%
!!! AAAAATTTGTTTTTATCTTG+TGG + chr2_2:40740936-40740955 None:intergenic 15.0%
!! TTCATTTGACAAATATATTT+GGG - chr2_2:40741041-40741060 Msa0251290:intron 15.0%
!!! AAACTAGTTTTAAATAATCT+TGG + chr2_2:40741765-40741784 None:intergenic 15.0%
!! TTAAACATGATTACATGTAT+CGG - chr2_2:40739284-40739303 Msa0251290:intron 20.0%
!! ATATTGTTGAAACTCAAAAT+TGG - chr2_2:40739450-40739469 Msa0251290:intron 20.0%
!!! TGGAATTTTTATCAATATTG+CGG - chr2_2:40739470-40739489 Msa0251290:intron 20.0%
!!! TTTGAACTTCTAATTTTAAG+TGG - chr2_2:40739600-40739619 Msa0251290:intron 20.0%
!!! ATTTTTACATTTTTTGCTCT+AGG - chr2_2:40739733-40739752 Msa0251290:intron 20.0%
!!! TTTTTACATTTTTTGCTCTA+GGG - chr2_2:40739734-40739753 Msa0251290:intron 20.0%
!! TAACTTTAAATTTATGTGCA+GGG - chr2_2:40739763-40739782 Msa0251290:intron 20.0%
!!! TCTTACAAAATTGTTTTCAA+TGG - chr2_2:40740955-40740974 Msa0251290:intron 20.0%
!! AAGTATTAGTGAATACTTTA+TGG + chr2_2:40740981-40741000 None:intergenic 20.0%
!! GTTCATTTGACAAATATATT+TGG - chr2_2:40741040-40741059 Msa0251290:intron 20.0%
!! TCATTTGACAAATATATTTG+GGG - chr2_2:40741042-40741061 Msa0251290:intron 20.0%
!! ATCTCAAATCAATACAAATT+TGG - chr2_2:40741084-40741103 Msa0251290:intron 20.0%
!! AACAAGAAAAATGTTACTAA+TGG - chr2_2:40741522-40741541 Msa0251290:CDS 20.0%
!!! AAAGTGTATGAGATTTTTTG+AGG + chr2_2:40738148-40738167 None:intergenic 25.0%
!! GGAGTTTTCATTATGTTTTT+TGG + chr2_2:40738516-40738535 None:intergenic 25.0%
!!! AAGTCAAAGAGTTTTAAAGA+AGG - chr2_2:40738552-40738571 Msa0251290:three_prime_UTR 25.0%
!!! AGTCAAAGAGTTTTAAAGAA+GGG - chr2_2:40738553-40738572 Msa0251290:three_prime_UTR 25.0%
! AAAATGAAGATTGCTCATTA+TGG - chr2_2:40738687-40738706 Msa0251290:three_prime_UTR 25.0%
! ATTGTACCAAGTATCAAAAA+GGG + chr2_2:40739042-40739061 None:intergenic 25.0%
! AATTGTACCAAGTATCAAAA+AGG + chr2_2:40739043-40739062 None:intergenic 25.0%
! TCATGATAAGCAATATAGAT+AGG + chr2_2:40739122-40739141 None:intergenic 25.0%
! AGTTTATTGAATGGATAGTT+CGG - chr2_2:40739406-40739425 Msa0251290:intron 25.0%
!! AATTTTTATCAATATTGCGG+TGG - chr2_2:40739473-40739492 Msa0251290:intron 25.0%
! AAGTGAGATTGACAAAATTA+TGG + chr2_2:40739503-40739522 None:intergenic 25.0%
! CTAACTTTAAATTTATGTGC+AGG - chr2_2:40739762-40739781 Msa0251290:intron 25.0%
! ATGTCTATTAGTTCAGAATA+TGG - chr2_2:40739842-40739861 Msa0251290:intron 25.0%
! ATTGAAATAAGCAAAGTTAG+AGG - chr2_2:40739866-40739885 Msa0251290:intron 25.0%
!! TAATGCTAACCAAATTTTAC+AGG - chr2_2:40739898-40739917 Msa0251290:intron 25.0%
! AACAGAAACTTATAAGCTAT+TGG + chr2_2:40740115-40740134 None:intergenic 25.0%
!!! TTGCAGTTAATTTTAAGACA+AGG - chr2_2:40740233-40740252 Msa0251290:CDS 25.0%
! TTTAGTTGTTTATCAAGATG+CGG + chr2_2:40740260-40740279 None:intergenic 25.0%
! AACTTATCTACATAAGTTCT+AGG + chr2_2:40740403-40740422 None:intergenic 25.0%
! CACTAATCAACATATTTAGT+CGG - chr2_2:40740442-40740461 Msa0251290:intron 25.0%
! ATGTATTTACAATGAGTCAA+AGG - chr2_2:40740576-40740595 Msa0251290:intron 25.0%
! AGTCAAAGGTTTATATACAA+AGG - chr2_2:40740590-40740609 Msa0251290:intron 25.0%
! GGTTTATATACAAAGGTAAA+CGG - chr2_2:40740597-40740616 Msa0251290:intron 25.0%
!!! TTTTGAAATTATGCCATTAC+AGG - chr2_2:40741002-40741021 Msa0251290:CDS 25.0%
! CATTTGACAAATATATTTGG+GGG - chr2_2:40741043-40741062 Msa0251290:intron 25.0%
! TGTTGTGTATAGTATTAGAT+AGG - chr2_2:40741402-40741421 Msa0251290:CDS 25.0%
!!! TGTTACTAATGGTGTTATTA+AGG - chr2_2:40741533-40741552 Msa0251290:CDS 25.0%
!!! GTTACTAATGGTGTTATTAA+GGG - chr2_2:40741534-40741553 Msa0251290:CDS 25.0%
! GTTATTAAGGGAAATAGATT+TGG - chr2_2:40741546-40741565 Msa0251290:CDS 25.0%
! CGATCTAATATCAGATTTAA+CGG - chr2_2:40741788-40741807 Msa0251290:CDS 25.0%
! TTCTTTCAAGATTTGGAACA+TGG + chr2_2:40738302-40738321 None:intergenic 30.0%
GAGTTTCTTCTTTCAAGATT+TGG + chr2_2:40738309-40738328 None:intergenic 30.0%
TTTCCTCTCTTTATAGCTAA+TGG + chr2_2:40738450-40738469 None:intergenic 30.0%
AAGAGAGGAAATGACAATAA+TGG - chr2_2:40738459-40738478 Msa0251290:three_prime_UTR 30.0%
CATAATGAAAACTCCAACTT+TGG - chr2_2:40738521-40738540 Msa0251290:three_prime_UTR 30.0%
!!! AGTTTTAAAGAAGGGTCTTT+TGG - chr2_2:40738561-40738580 Msa0251290:three_prime_UTR 30.0%
TAACTACCAATTACCTGAAT+TGG + chr2_2:40738820-40738839 None:intergenic 30.0%
! TATGTACCCTTTTTGATACT+TGG - chr2_2:40739033-40739052 Msa0251290:CDS 30.0%
TTGCTTATCATGATGAAGAA+TGG - chr2_2:40739129-40739148 Msa0251290:intron 30.0%
TGCTTATCATGATGAAGAAT+GGG - chr2_2:40739130-40739149 Msa0251290:intron 30.0%
ATTTATGCCAGTGTTTCATA+AGG + chr2_2:40739210-40739229 None:intergenic 30.0%
ACGTAAACTAAGTGTATGTT+TGG - chr2_2:40739380-40739399 Msa0251290:intron 30.0%
GTTTGGATGAGTTTATTGAA+TGG - chr2_2:40739397-40739416 Msa0251290:intron 30.0%
TTATGTTGTTGTCCATATTC+AGG - chr2_2:40739571-40739590 Msa0251290:intron 30.0%
TTCAAACAACATCCTGAATA+TGG + chr2_2:40739586-40739605 None:intergenic 30.0%
CATAATCAAACCTGAAATCT+AGG + chr2_2:40739668-40739687 None:intergenic 30.0%
AGAATTTGATGCAGAAATTG+TGG - chr2_2:40739796-40739815 Msa0251290:intron 30.0%
CATTTGCTTATCAGTCAAAT+TGG + chr2_2:40739822-40739841 None:intergenic 30.0%
GAACTTCTAGTAGAAAAGAA+CGG - chr2_2:40740006-40740025 Msa0251290:intron 30.0%
ATTGATCAATCAACTCATGT+GGG + chr2_2:40740144-40740163 None:intergenic 30.0%
!! AATATGTGACAGCATTGATA+AGG + chr2_2:40740180-40740199 None:intergenic 30.0%
TTAGTTGTTTATCAAGATGC+GGG + chr2_2:40740259-40740278 None:intergenic 30.0%
ATATACAAAGGTAAACGGTA+CGG - chr2_2:40740602-40740621 Msa0251290:intron 30.0%
!! ATTAGTTAATTAGAGCACTG+AGG + chr2_2:40740660-40740679 None:intergenic 30.0%
ATATAAGCTATCCTTACCAA+TGG - chr2_2:40740896-40740915 Msa0251290:intron 30.0%
ATAAATGAATGAACCACTGT+TGG + chr2_2:40741189-40741208 None:intergenic 30.0%
TGATTAAGTGGTCATTTGTT+AGG + chr2_2:40741224-40741243 None:intergenic 30.0%
GGGTCAATTAATCTCTTATA+TGG + chr2_2:40741283-40741302 None:intergenic 30.0%
! TTATGTCTTCAAGTCTTGTA+GGG + chr2_2:40741303-40741322 None:intergenic 30.0%
! TTTATGTCTTCAAGTCTTGT+AGG + chr2_2:40741304-40741323 None:intergenic 30.0%
! CAGAAGGAAAAAACACTTTT+AGG + chr2_2:40741428-40741447 None:intergenic 30.0%
AAATAATGCAAAGTCCTTGT+AGG + chr2_2:40741983-40742002 None:intergenic 30.0%
TACAAGGACTTTGCATTATT+TGG - chr2_2:40741982-40742001 Msa0251290:five_prime_UTR 30.0%
!! ATACTTGTTGAAGAGTGTTT+AGG - chr2_2:40742028-40742047 Msa0251290:five_prime_UTR 30.0%
!! TACTTGTTGAAGAGTGTTTA+GGG - chr2_2:40742029-40742048 Msa0251290:five_prime_UTR 30.0%
AAATGAGGGAAATGTTGTGA+GGG + chr2_2:40738088-40738107 None:intergenic 35.0%
AAAATGAGGGAAATGTTGTG+AGG + chr2_2:40738089-40738108 None:intergenic 35.0%
GAAAAGAGAGAGGAAAATGA+GGG + chr2_2:40738102-40738121 None:intergenic 35.0%
TGAAAAGAGAGAGGAAAATG+AGG + chr2_2:40738103-40738122 None:intergenic 35.0%
AGATTGCAATGAAAAGAGAG+AGG + chr2_2:40738112-40738131 None:intergenic 35.0%
TACAGTTACTTCTTTAGCCT+TGG + chr2_2:40738208-40738227 None:intergenic 35.0%
AAGGCTAAAGAAGTAACTGT+AGG - chr2_2:40738207-40738226 Msa0251290:three_prime_UTR 35.0%
ATGTTGCTAGAATCAATGGA+AGG - chr2_2:40738253-40738272 Msa0251290:three_prime_UTR 35.0%
!!! GGTAATGGAGAAAGTGATTT+TGG + chr2_2:40738348-40738367 None:intergenic 35.0%
!! GAAGTAGTAGTAGTCTTGTT+TGG + chr2_2:40738375-40738394 None:intergenic 35.0%
!!! TTTGGTTTTGGAGAAATTGG+TGG + chr2_2:40738408-40738427 None:intergenic 35.0%
!!! GACTTTGGTTTTGGAGAAAT+TGG + chr2_2:40738411-40738430 None:intergenic 35.0%
AGACCATTAGCTATAAAGAG+AGG - chr2_2:40738444-40738463 Msa0251290:three_prime_UTR 35.0%
!! TTCTTTGATCACTGATTCAC+CGG - chr2_2:40738611-40738630 Msa0251290:three_prime_UTR 35.0%
!! TTTGAATCAAGAGCACTTGA+AGG + chr2_2:40738756-40738775 None:intergenic 35.0%
TTCAAAGACTACCAATGAAG+AGG - chr2_2:40738770-40738789 Msa0251290:three_prime_UTR 35.0%
GCTTATCATGATGAAGAATG+GGG - chr2_2:40739131-40739150 Msa0251290:intron 35.0%
! CACGTTTTCAATCATAAGTG+TGG - chr2_2:40739342-40739361 Msa0251290:intron 35.0%
ACTTAGTTTACGTGTGTTTG+TGG + chr2_2:40739373-40739392 None:intergenic 35.0%
!! ATTTTAAGTGGAGCTCAAGT+TGG - chr2_2:40739612-40739631 Msa0251290:intron 35.0%
!!! GAAATCTAGGCGTTTTTTCA+AGG + chr2_2:40739655-40739674 None:intergenic 35.0%
TGAAAAAACGCCTAGATTTC+AGG - chr2_2:40739655-40739674 Msa0251290:intron 35.0%
AATTCACTCGTTGCCAAAAA+AGG - chr2_2:40740072-40740091 Msa0251290:intron 35.0%
TTGATCAATCAACTCATGTG+GGG + chr2_2:40740143-40740162 None:intergenic 35.0%
CATTGATCAATCAACTCATG+TGG + chr2_2:40740145-40740164 None:intergenic 35.0%
TTTAGTCGGTTAGAACACTT+AGG - chr2_2:40740456-40740475 Msa0251290:intron 35.0%
GACTTGCACACAAAGATAAT+GGG + chr2_2:40740516-40740535 None:intergenic 35.0%
AGACTTGCACACAAAGATAA+TGG + chr2_2:40740517-40740536 None:intergenic 35.0%
CTTTCTTTCTCTCTTCTTGT+TGG + chr2_2:40740826-40740845 None:intergenic 35.0%
AAACACTTGTACCATTGGTA+AGG + chr2_2:40740910-40740929 None:intergenic 35.0%
TACCAATGGTACAAGTGTTT+AGG - chr2_2:40740910-40740929 Msa0251290:intron 35.0%
ATGCCATTACAGGTTAAGAA+AGG - chr2_2:40741012-40741031 Msa0251290:intron 35.0%
AAGCCTTTCTTAACCTGTAA+TGG + chr2_2:40741018-40741037 None:intergenic 35.0%
TTACAGGTTAAGAAAGGCTT+TGG - chr2_2:40741018-40741037 Msa0251290:intron 35.0%
!! TCTGATTTTGATGTCTTCAC+TGG + chr2_2:40741120-40741139 None:intergenic 35.0%
AGCAAAGACATGATTAAGAG+AGG - chr2_2:40741147-40741166 Msa0251290:intron 35.0%
GCAAAGACATGATTAAGAGA+GGG - chr2_2:40741148-40741167 Msa0251290:intron 35.0%
AAGAGAGGGTTTAGATATGT+TGG - chr2_2:40741162-40741181 Msa0251290:intron 35.0%
AGTGGTTCATTCATTTATGC+AGG - chr2_2:40741191-40741210 Msa0251290:intron 35.0%
!! TGTGCTGACTATAGTTTACT+TGG - chr2_2:40741372-40741391 Msa0251290:CDS 35.0%
AAATGATCTTGATCTTGCGT+CGG + chr2_2:40741665-40741684 None:intergenic 35.0%
AAATGTTGTGAGGGATTGGA+GGG + chr2_2:40738079-40738098 None:intergenic 40.0%
ACAAAACATGTGTAGCTCCA+AGG - chr2_2:40738188-40738207 Msa0251290:three_prime_UTR 40.0%
!! CCATTGATTCTAGCAACATG+TGG + chr2_2:40738252-40738271 None:intergenic 40.0%
CCACATGTTGCTAGAATCAA+TGG - chr2_2:40738249-40738268 Msa0251290:three_prime_UTR 40.0%
! TTGCATGTTGGTTGAAGTAC+TGG + chr2_2:40738279-40738298 None:intergenic 40.0%
! TTTGGAACATGGTTGCATGT+TGG + chr2_2:40738291-40738310 None:intergenic 40.0%
!! GTCTTGTTTGGAAGTGGTAA+TGG + chr2_2:40738363-40738382 None:intergenic 40.0%
!! GTAGTAGTCTTGTTTGGAAG+TGG + chr2_2:40738369-40738388 None:intergenic 40.0%
!! CTAGTAGGTGACTTTGGTTT+TGG + chr2_2:40738420-40738439 None:intergenic 40.0%
CTAGTACTAGTAGGTGACTT+TGG + chr2_2:40738426-40738445 None:intergenic 40.0%
GCTAATGGTCTAGTACTAGT+AGG + chr2_2:40738435-40738454 None:intergenic 40.0%
! TGAAAACTCCAACTTTGGAG+AGG - chr2_2:40738526-40738545 Msa0251290:three_prime_UTR 40.0%
!! GACTTTTTCCTCTCCAAAGT+TGG + chr2_2:40738537-40738556 None:intergenic 40.0%
!! TCTTTGATCACTGATTCACC+GGG - chr2_2:40738612-40738631 Msa0251290:three_prime_UTR 40.0%
AAGCATAGCTGCTGTTAGAA+GGG - chr2_2:40738635-40738654 Msa0251290:three_prime_UTR 40.0%
! AGAGCACTTGAAGGATCAAT+AGG + chr2_2:40738747-40738766 None:intergenic 40.0%
TCACAGCCAATTCAGGTAAT+TGG - chr2_2:40738811-40738830 Msa0251290:three_prime_UTR 40.0%
GCTTACTTGTCATCATGAAC+TGG + chr2_2:40739161-40739180 None:intergenic 40.0%
ACACACACCTTATGAAACAC+TGG - chr2_2:40739200-40739219 Msa0251290:intron 40.0%
ACACTGACACATGTTAGAGA+CGG - chr2_2:40739318-40739337 Msa0251290:intron 40.0%
TGGATAGTTCGGCAAAATCA+TGG - chr2_2:40739417-40739436 Msa0251290:intron 40.0%
!!! TAGGCGTTTTTTCAAGGTTG+AGG + chr2_2:40739649-40739668 None:intergenic 40.0%
!!! AACTTGTCTGCAGCCTTTTT+TGG + chr2_2:40740088-40740107 None:intergenic 40.0%
TTCTCTATATCAGCACTCAC+TGG + chr2_2:40740695-40740714 None:intergenic 40.0%
TAGATATGTTGGTCCAACAG+TGG - chr2_2:40741173-40741192 Msa0251290:intron 40.0%
GCACTTGCAATGCACATTAT+TGG - chr2_2:40741254-40741273 Msa0251290:intron 40.0%
ACGACATCTCACAAATCAGA+AGG + chr2_2:40741444-40741463 None:intergenic 40.0%
!!! TAGAAGCGTGCTTTTTCAAC+AGG - chr2_2:40741832-40741851 Msa0251290:CDS 40.0%
!!! AGAAGCGTGCTTTTTCAACA+GGG - chr2_2:40741833-40741852 Msa0251290:CDS 40.0%
CATGTGCATGTGCAAATAGA+AGG - chr2_2:40741903-40741922 Msa0251290:CDS 40.0%
CATGTGCAAATAGAAGGCAA+GGG - chr2_2:40741909-40741928 Msa0251290:CDS 40.0%
TTGTAGGTCACAATGTCACA+TGG + chr2_2:40741967-40741986 None:intergenic 40.0%
ATGTGACATTGTGACCTACA+AGG - chr2_2:40741966-40741985 Msa0251290:five_prime_UTR 40.0%
GAAATGTTGTGAGGGATTGG+AGG + chr2_2:40738080-40738099 None:intergenic 45.0%
AGGGAAATGTTGTGAGGGAT+TGG + chr2_2:40738083-40738102 None:intergenic 45.0%
! TCTAGCAACATGTGGTGTTG+TGG + chr2_2:40738244-40738263 None:intergenic 45.0%
GAAGCATAGCTGCTGTTAGA+AGG - chr2_2:40738634-40738653 Msa0251290:three_prime_UTR 45.0%
TGCTGTTAGAAGGGAACAAG+TGG - chr2_2:40738644-40738663 Msa0251290:three_prime_UTR 45.0%
TGGCACTTCAACAAGCACAA+AGG - chr2_2:40738664-40738683 Msa0251290:three_prime_UTR 45.0%
GCATCTCTTCTCCTCTTCAT+TGG + chr2_2:40738784-40738803 None:intergenic 45.0%
AGCTTCATCACAGCCAATTC+AGG - chr2_2:40738804-40738823 Msa0251290:three_prime_UTR 45.0%
! TATCACCTGTGTCGATGTCT+CGG + chr2_2:40739253-40739272 None:intergenic 45.0%
GTAAACGGTACGGATGTTAG+AGG - chr2_2:40740612-40740631 Msa0251290:intron 45.0%
GGCCTAAACACTTGTACCAT+TGG + chr2_2:40740915-40740934 None:intergenic 45.0%
CATTCATTTATGCAGGCAGC+TGG - chr2_2:40741198-40741217 Msa0251290:intron 45.0%
ACCACTTAATCACTTGCCAC+AGG - chr2_2:40741232-40741251 Msa0251290:intron 45.0%
!!! GAAGCGTGCTTTTTCAACAG+GGG - chr2_2:40741834-40741853 Msa0251290:CDS 45.0%
GGAATCCGTTAAGAAGTGGT+GGG - chr2_2:40741855-40741874 Msa0251290:CDS 45.0%
ACACTCCCACCACTTCTTAA+CGG + chr2_2:40741863-40741882 None:intergenic 45.0%
!! TAAGAAGTGGTGGGAGTGTT+AGG - chr2_2:40741864-40741883 Msa0251290:CDS 45.0%
!! AAGAAGTGGTGGGAGTGTTA+GGG - chr2_2:40741865-40741884 Msa0251290:CDS 45.0%
GCATGTGCAAATAGAAGGCA+AGG - chr2_2:40741908-40741927 Msa0251290:CDS 45.0%
TTCCCTTTAACAGCCTGTGT+GGG - chr2_2:40742005-40742024 Msa0251290:five_prime_UTR 45.0%
ATCCCACACAGGCTGTTAAA+GGG + chr2_2:40742010-40742029 None:intergenic 45.0%
TATCCCACACAGGCTGTTAA+AGG + chr2_2:40742011-40742030 None:intergenic 45.0%
CTTCAACAAGTATCCCACAC+AGG + chr2_2:40742021-40742040 None:intergenic 45.0%
!! AAAATTAAAATAATAAGAAA+TGG + chr2_2:40740051-40740070 None:intergenic 5.0%
CTAACAGCAGCTATGCTTCC+CGG + chr2_2:40738633-40738652 None:intergenic 50.0%
AGGTGTGTGTGAGAGAGAGA+GGG + chr2_2:40739190-40739209 None:intergenic 50.0%
AAGGTGTGTGTGAGAGAGAG+AGG + chr2_2:40739191-40739210 None:intergenic 50.0%
!! GAGCTCAAGTTGGATCTGAC+TGG - chr2_2:40739622-40739641 Msa0251290:intron 50.0%
GCCTGTGGCAAGTGATTAAG+TGG + chr2_2:40741236-40741255 None:intergenic 50.0%
ATGTGCATTGCAAGTGCCTG+TGG + chr2_2:40741251-40741270 None:intergenic 50.0%
GTGCATTAACTGCGTCAAGC+AGG - chr2_2:40741568-40741587 Msa0251290:CDS 50.0%
CAGGGGAATCCGTTAAGAAG+TGG - chr2_2:40741851-40741870 Msa0251290:CDS 50.0%
GGGAATCCGTTAAGAAGTGG+TGG - chr2_2:40741854-40741873 Msa0251290:CDS 50.0%
GTTCCCTTTAACAGCCTGTG+TGG - chr2_2:40742004-40742023 Msa0251290:five_prime_UTR 50.0%
TGTGAGAGAGAGAGGGGAAG+GGG + chr2_2:40739183-40739202 None:intergenic 55.0%
GTGTGAGAGAGAGAGGGGAA+GGG + chr2_2:40739184-40739203 None:intergenic 55.0%
TGTGTGAGAGAGAGAGGGGA+AGG + chr2_2:40739185-40739204 None:intergenic 55.0%
GGTGTGTGTGAGAGAGAGAG+GGG + chr2_2:40739189-40739208 None:intergenic 55.0%
CAACACCGAGACATCGACAC+AGG - chr2_2:40739245-40739264 Msa0251290:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 40738076 40742071 40738076 ID=Msa0251290;Name=Msa0251290
chr2_2 mRNA 40738076 40742071 40738076 ID=Msa0251290-mRNA-1;Parent=Msa0251290;Name=Msa0251290-mRNA-1;_AED=0.02;_eAED=0.02;_QI=119|1|1|1|1|1|5|784|390
chr2_2 exon 40738076 40739123 40738076 ID=Msa0251290-mRNA-1:exon:12706;Parent=Msa0251290-mRNA-1
chr2_2 exon 40740228 40740363 40740228 ID=Msa0251290-mRNA-1:exon:12705;Parent=Msa0251290-mRNA-1
chr2_2 exon 40740471 40740554 40740471 ID=Msa0251290-mRNA-1:exon:12704;Parent=Msa0251290-mRNA-1
chr2_2 exon 40740973 40741030 40740973 ID=Msa0251290-mRNA-1:exon:12703;Parent=Msa0251290-mRNA-1
chr2_2 exon 40741322 40742071 40741322 ID=Msa0251290-mRNA-1:exon:12702;Parent=Msa0251290-mRNA-1
chr2_2 five_prime_UTR 40741953 40742071 40741953 ID=Msa0251290-mRNA-1:five_prime_utr;Parent=Msa0251290-mRNA-1
chr2_2 CDS 40741322 40741952 40741322 ID=Msa0251290-mRNA-1:cds;Parent=Msa0251290-mRNA-1
chr2_2 CDS 40740973 40741030 40740973 ID=Msa0251290-mRNA-1:cds;Parent=Msa0251290-mRNA-1
chr2_2 CDS 40740471 40740554 40740471 ID=Msa0251290-mRNA-1:cds;Parent=Msa0251290-mRNA-1
chr2_2 CDS 40740228 40740363 40740228 ID=Msa0251290-mRNA-1:cds;Parent=Msa0251290-mRNA-1
chr2_2 CDS 40738860 40739123 40738860 ID=Msa0251290-mRNA-1:cds;Parent=Msa0251290-mRNA-1
chr2_2 three_prime_UTR 40738076 40738859 40738076 ID=Msa0251290-mRNA-1:three_prime_utr;Parent=Msa0251290-mRNA-1
Gene Sequence

>Msa0251290

ATGTGTAGCTCCAAGGCTAAAGAAGTAACTGTAGGCATAGAATCTTCCACAACACCACATGTTGCTAGAATCAATGGAAGGCCAGTACTTCAACCAACATGCAACCATGTTCCAAATCTTGAAAGAAGAAACTCAATTAAGAAATCAACACCAAAATCACTTTCTCCATTACCACTTCCAAACAAGACTACTACTACTTCTTCATTGACACCACCAATTTCTCCAAAACCAAAGTCACCTACTAGTACTAGACCATTAGCTATAAAGAGAGGAAATGACAATAATGGACTTAATTTAAGCTGTGAAAAGATTTCAATACCAAAAAACATAATGAAAACTCCAACTTTGGAGAGGAAAAAGTCAAAGAGTTTTAAAGAAGGGTCTTTTGGTATTGAAGCTTCATTGAGTTATTCTTCTTCTTTGATCACTGATTCACCGGGAAGCATAGCTGCTGTTAGAAGGGAACAAGTGGCACTTCAACAAGCACAAAGGAAAATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCTAAATTTGAAAGAGTTTTTCCTATTGATCCTTCAAGTGCTCTTGATTCAAAGACTACCAATGAAGAGGAGAAGAGATGCAGCTTCATCACAGCCAATTCAGATCCTATCTATATTGCTTATCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAAGATGTTGTTTGAACTTCTAATTTTAAGTGGAGCTCAAGTTGGATCTGACTGGACCTCAACCTTGAAAAAACGCCTAGATTTCAGGGCTGCATTTTCAGAATTTGATGCAGAAATTGTGGCCAATTTGACTGATAAGCAAATGATGTCTATTAGTTCAGAATATGGCATTGAAATAAGCAAAGTTAGAGGAGTTGTTGATAATGCTAACCAAATTTTACAGGTTAAGAAAGGCTTTGGTTCATTTGACAAATATATTTGGGGGTTTGTGAATCATAAACAAATCTCAAATCAATACAAATTTGGTCACAAGATTCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGATTAAGAGAGGGTTTAGATATGTTGGTCCAACAGTGGTTCATTCATTTATGCAGGCAGCTGGCCTAACAAATGACCACTTAATCACTTGCCACAGGCACTTGCAATGCACATTATTGGCAGCCATATAA
Protein sequence

>Msa0251290

MCSSKAKEVTVGIESSTTPHVARINGRPVLQPTCNHVPNLERRNSIKKSTPKSLSPLPLPNKTTTTSSLTPPISPKPKSPTSTRPLAIKRGNDNNGLNLSCEKISIPKNIMKTPTLERKKSKSFKEGSFGIEASLSYSSSLITDSPGSIAAVRREQVALQQAQRKMKIAHYGRSKSAKFERVFPIDPSSALDSKTTNEEEKRCSFITANSDPIYIAYHDEEWGVPVHDDKMLFELLILSGAQVGSDWTSTLKKRLDFRAAFSEFDAEIVANLTDKQMMSISSEYGIEISKVRGVVDNANQILQVKKGFGSFDKYIWGFVNHKQISNQYKFGHKIPVKTSKSESISKDMIKRGFRYVGPTVVHSFMQAAGLTNDHLITCHRHLQCTLLAAI*