AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0204760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0204760 MtrunA17_Chr2g0309681 98.465 391 4 2 1 390 1 390 0.0 789
Msa0204760 MtrunA17_Chr8g0356301 77.665 394 72 7 1 389 1 383 0.0 574
Msa0204760 MtrunA17_Chr4g0073991 51.813 193 91 1 198 388 145 337 2.90e-69 223
Msa0204760 MtrunA17_Chr5g0420791 48.958 192 96 1 199 388 110 301 2.77e-68 218
Msa0204760 MtrunA17_Chr8g0366071 48.718 195 95 2 199 388 114 308 1.28e-67 216
Msa0204760 MtrunA17_Chr4g0001371 49.738 191 94 1 198 386 134 324 3.83e-67 217
Msa0204760 MtrunA17_Chr3g0141411 46.154 221 115 3 169 386 97 316 2.33e-65 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0204760 AT3G12710.1 70.758 277 65 3 115 388 46 309 4.59e-139 399
Msa0204760 AT5G44680.1 51.523 394 146 8 1 390 1 353 1.33e-135 392
Msa0204760 AT1G75090.1 53.723 188 85 1 201 386 119 306 6.93e-74 233
Msa0204760 AT5G57970.1 50.472 212 97 3 183 386 130 341 1.89e-71 228
Msa0204760 AT5G57970.2 50.472 212 97 3 183 386 130 341 1.89e-71 228
Msa0204760 AT1G80850.1 54.839 186 82 1 201 384 134 319 4.94e-71 226
Msa0204760 AT1G13635.2 49.020 204 102 1 187 388 101 304 5.14e-71 225
Msa0204760 AT1G13635.1 49.020 204 102 1 187 388 101 304 5.14e-71 225
Msa0204760 AT1G15970.1 50.769 195 84 2 201 388 145 334 1.40e-66 215
Msa0204760 AT1G15970.2 50.769 195 84 2 201 388 145 334 1.40e-66 215

Find 105 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAAGGACTTTGCATTATT+TGG 0.099995 2_1:+30567106 Msa0204760:three_prime_UTR
GCACTTGCAATGCACATTAT+TGG 0.261232 2_1:+30566391 Msa0204760:CDS
CATTTGTTTATCAGTCAAAT+TGG 0.271540 2_1:-30564958 None:intergenic
TTCATTTGACAAATACATTT+GGG 0.277104 2_1:+30566178 Msa0204760:CDS
TAACTATCAATTACCTGAAT+TGG 0.293308 2_1:-30563964 None:intergenic
TAAGAAGTGGTGGGAGTGTT+AGG 0.297982 2_1:+30567002 Msa0204760:three_prime_UTR
GGTAATGGAGAAAGTGATTT+TGG 0.299287 2_1:-30563492 None:intergenic
ATCTCAAATCAATACAAATT+TGG 0.303182 2_1:+30566221 Msa0204760:CDS
TTACAGGTTAAGAAAGTCTT+TGG 0.305623 2_1:+30566155 Msa0204760:intron
TTTCCTCTCTTTATAGCTAA+TGG 0.311064 2_1:-30563594 None:intergenic
CTAGTAGGTGACTTTGGTTT+TGG 0.311738 2_1:-30563564 None:intergenic
AAAATGAAGATTGCTCATTA+TGG 0.312266 2_1:+30563834 Msa0204760:CDS
TATCCCACACAGGCTGTTAA+AGG 0.317111 2_1:-30567132 None:intergenic
GAGTTTCTTCTTTCAAGATT+TGG 0.322751 2_1:-30563453 None:intergenic
CATAATGAAAACTCCAACTT+TGG 0.328226 2_1:+30563668 Msa0204760:CDS
TTTAAGTCTTGAAAACTTGT+AGG 0.329495 2_1:-30566438 None:intergenic
GGGTCAATTAATCTCTTATA+TGG 0.340630 2_1:-30566417 None:intergenic
GTTCATTTGACAAATACATT+TGG 0.343231 2_1:+30566177 Msa0204760:CDS
TGATTAAGTGGTCATTTGTT+AGG 0.343650 2_1:-30566358 None:intergenic
GAAGCATAGCTGCTGTTAGA+AGG 0.354512 2_1:+30563781 Msa0204760:CDS
TACAGTTACTTCTTTAGCCT+TGG 0.358427 2_1:-30563352 None:intergenic
CGATTTGATATCAGATTTAA+CGG 0.362232 2_1:+30566927 Msa0204760:three_prime_UTR
ATGTCTATTAGTTCAGAATA+TGG 0.363323 2_1:+30564981 Msa0204760:CDS
GAAGTAGTAGTAGTCTTGTT+TGG 0.369631 2_1:-30563519 None:intergenic
AAGAGAGGAAATGACAATAA+TGG 0.373376 2_1:+30563606 Msa0204760:CDS
CAGCATGTGCATGTGCAAAT+AGG 0.375156 2_1:+30567032 Msa0204760:three_prime_UTR
ACACTCCCACCACTTCTTAA+CGG 0.386475 2_1:-30566998 None:intergenic
AGAGAAAATAGGGTTTGTTA+AGG 0.386686 2_1:-30567180 None:intergenic
AGGGAAATGTTGTGAGGGAT+TGG 0.388202 2_1:-30563227 None:intergenic
CTAACAGCAGCTATGCTTCC+CGG 0.396009 2_1:-30563777 None:intergenic
TGCTTATCATGATGAAGAAT+GGG 0.396248 2_1:+30564279 Msa0204760:CDS
GTCTTGTTTGGAAGTGGTAA+TGG 0.397911 2_1:-30563507 None:intergenic
TTCTTTGATCACTGATTCAC+CGG 0.407327 2_1:+30563758 Msa0204760:CDS
AGAGCACTTGAAGGATCAAT+AGG 0.410745 2_1:-30563891 None:intergenic
TGTTGTGTATAGTATTAGAT+AGG 0.416676 2_1:+30566539 Msa0204760:three_prime_UTR
TGTTACTAATGGTGTTATCA+AGG 0.418646 2_1:+30566670 Msa0204760:three_prime_UTR
AATTTGTATTGATTTGAGAT+TGG 0.419291 2_1:-30566218 None:intergenic
AAATAATGCAAAGTCCTTGT+AGG 0.428444 2_1:-30567104 None:intergenic
CTAGTACTAGTAGGTGACTT+TGG 0.431001 2_1:-30563570 None:intergenic
TGTGCTGACTATAGTTTACT+TGG 0.436773 2_1:+30566509 Msa0204760:three_prime_UTR
CATTCATTTATGCAGGCAGC+TGG 0.444232 2_1:+30566335 Msa0204760:CDS
TTGCTTATCATGATGAAGAA+TGG 0.451345 2_1:+30564278 Msa0204760:CDS
TCATGATAAGCAATATAGAT+AGG 0.456509 2_1:-30564268 None:intergenic
TTGCATGTTGGTTGAAGTAC+TGG 0.458812 2_1:-30563423 None:intergenic
TTTGAATCAAGAGCACTTGA+AGG 0.459539 2_1:-30563900 None:intergenic
CCACATGTTGCTAGAATCAA+TGG 0.460004 2_1:+30563396 Msa0204760:CDS
TTAAGTCTTGAAAACTTGTA+GGG 0.467648 2_1:-30566437 None:intergenic
GTAGTAGTCTTGTTTGGAAG+TGG 0.468780 2_1:-30563513 None:intergenic
TGAAAAGAGAGAGGAAAATG+AGG 0.479288 2_1:-30563247 None:intergenic
AAGAGAGGGTTTAGATATGT+TGG 0.481696 2_1:+30566299 Msa0204760:CDS
GCTTACTTGTCATCATGAAC+TGG 0.481850 2_1:-30564307 None:intergenic
GCTAATGGTCTAGTACTAGT+AGG 0.483572 2_1:-30563579 None:intergenic
AGCTTCATCACAGCCAATTC+AGG 0.489518 2_1:+30563951 Msa0204760:CDS
TTCTTTCAAGATTTGGAACA+TGG 0.493504 2_1:-30563446 None:intergenic
ATTGATATAAGCAAAGTTAG+AGG 0.493859 2_1:+30565005 Msa0204760:CDS
GAAAATAGGGTTTGTTAAGG+TGG 0.494645 2_1:-30567177 None:intergenic
GAAATGTTGTGAGGGATTGG+AGG 0.499447 2_1:-30563224 None:intergenic
AGTGGTTCATTCATTTATGC+AGG 0.501189 2_1:+30566328 Msa0204760:CDS
AGAATTTGATGCAGAAATTG+TGG 0.503076 2_1:+30564935 Msa0204760:CDS
CATAATCAAACCTGAAGTCT+AGG 0.506796 2_1:-30564806 None:intergenic
AGCAAAGACATGATTAAGAG+AGG 0.510022 2_1:+30566284 Msa0204760:CDS
AAGAAGTGGTGGGAGTGTTA+GGG 0.512402 2_1:+30567003 Msa0204760:three_prime_UTR
TGTGCATTAACTACTCAAGC+AGG 0.518453 2_1:+30566704 Msa0204760:three_prime_UTR
AAATGTTGTGAGGGATTGGA+GGG 0.520939 2_1:-30563223 None:intergenic
GAAAAGAGAGAGGAAAATGA+GGG 0.533265 2_1:-30563246 None:intergenic
GTGCTCAAGTTGGATCTGAT+TGG 0.533668 2_1:+30564763 Msa0204760:CDS
GTTTCCTTTAACAGCCTGTG+TGG 0.545229 2_1:+30567128 Msa0204760:three_prime_UTR
TACTTGTTGAAGAGTGTGTA+GGG 0.548034 2_1:+30567153 Msa0204760:three_prime_UTR
GTTACTAATGGTGTTATCAA+GGG 0.551429 2_1:+30566671 Msa0204760:three_prime_UTR
GAGAATCCGTTAAGAAGTGG+TGG 0.558955 2_1:+30566992 Msa0204760:three_prime_UTR
GCAAAGACATGATTAAGAGA+GGG 0.567917 2_1:+30566285 Msa0204760:CDS
GCATGTGCAAATAGGAGGCA+AGG 0.570710 2_1:+30567040 Msa0204760:three_prime_UTR
CAAAGTCCTTGTAGGTCACA+TGG 0.570861 2_1:-30567096 None:intergenic
AAGCATAGCTGCTGTTAGAA+GGG 0.572624 2_1:+30563782 Msa0204760:CDS
TTTCCTTTAACAGCCTGTGT+GGG 0.576044 2_1:+30567129 Msa0204760:three_prime_UTR
TTCAAAGACTACTAATCAAG+AGG 0.580713 2_1:+30563917 Msa0204760:CDS
GCCTGTGGCAAGTGATTAAG+TGG 0.581468 2_1:-30566370 None:intergenic
TCATTTGACAAATACATTTG+GGG 0.586610 2_1:+30566179 Msa0204760:CDS
TGCTGTTAGAAGGGAACAAG+TGG 0.590285 2_1:+30563791 Msa0204760:CDS
CATGTGCATGTGCAAATAGG+AGG 0.591580 2_1:+30567035 Msa0204760:three_prime_UTR
TCTAGCAACATGTGGTGTTG+TGG 0.592032 2_1:-30563388 None:intergenic
CATTTGACAAATACATTTGG+GGG 0.598419 2_1:+30566180 Msa0204760:CDS
ATAAATGAATGAACCACTGT+TGG 0.599012 2_1:-30566323 None:intergenic
CATGTGCAAATAGGAGGCAA+GGG 0.600226 2_1:+30567041 Msa0204760:three_prime_UTR
AGATTGCAATGAAAAGAGAG+AGG 0.603429 2_1:-30563256 None:intergenic
AAATGAGGGAAATGTTGTGA+GGG 0.604544 2_1:-30563232 None:intergenic
GAGAGACCATGTGACCTACA+AGG 0.606245 2_1:+30567090 Msa0204760:three_prime_UTR
AAGGCTAAAGAAGTAACTGT+AGG 0.608755 2_1:+30563354 Msa0204760:CDS
ACCACTTAATCACTTGCCAC+AGG 0.609569 2_1:+30566369 Msa0204760:CDS
ACAAAACATGTGTAGCTCCA+AGG 0.617528 2_1:+30563335 Msa0204760:exon
CAGGAGAATCCGTTAAGAAG+TGG 0.619282 2_1:+30566989 Msa0204760:three_prime_UTR
TAGATATGTTGGTCCAACAG+TGG 0.633858 2_1:+30566310 Msa0204760:CDS
CCATTGATTCTAGCAACATG+TGG 0.642807 2_1:-30563396 None:intergenic
TTTGGAACATGGTTGCATGT+TGG 0.648256 2_1:-30563435 None:intergenic
TGAAAACTCCAACTTTGGAG+AGG 0.649673 2_1:+30563673 Msa0204760:CDS
AAAATGAGGGAAATGTTGTG+AGG 0.657957 2_1:-30563233 None:intergenic
ATACTTGTTGAAGAGTGTGT+AGG 0.661793 2_1:+30567152 Msa0204760:three_prime_UTR
AGACCATTAGCTATAAAGAG+AGG 0.661843 2_1:+30563591 Msa0204760:CDS
ATGTGCATTGCAAGTGCCTG+TGG 0.666497 2_1:-30566385 None:intergenic
AGAATCCGTTAAGAAGTGGT+GGG 0.668441 2_1:+30566993 Msa0204760:three_prime_UTR
GCTTATCATGATGAAGAATG+GGG 0.675468 2_1:+30564280 Msa0204760:CDS
ATGTTGCTAGAATCAATGGA+AGG 0.678120 2_1:+30563400 Msa0204760:CDS
TCTTTGATCACTGATTCACC+GGG 0.686123 2_1:+30563759 Msa0204760:CDS
TGGCACTTCAGCAAGCACAA+AGG 0.706629 2_1:+30563811 Msa0204760:CDS
CTTCAACAAGTATCCCACAC+AGG 0.710515 2_1:-30567142 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATAGTTTAATTTATTAT+AGG + chr2_1:30564120-30564139 Msa0204760:intron 10.0%
!! AAAATTAAAATAATAAGAAG+TGG - chr2_1:30565190-30565209 None:intergenic 10.0%
!!! TTTTAACTTTTAAATAATCT+TGG - chr2_1:30566897-30566916 None:intergenic 10.0%
!!! TTTTTTTTACCTGTAAAATT+TGG - chr2_1:30565049-30565068 None:intergenic 15.0%
!!! AAAAAATTGTTTTTATCTTG+TGG - chr2_1:30566073-30566092 None:intergenic 15.0%
!!! TCTTAAAAATTTGTTTTCAA+TGG + chr2_1:30566092-30566111 Msa0204760:intron 15.0%
!!! AAATCAATTTTAAATGATCT+CGG - chr2_1:30566746-30566765 None:intergenic 15.0%
!! GCACTAAGTATAAAAAAAAA+GGG - chr2_1:30564189-30564208 None:intergenic 20.0%
!! TGCACTAAGTATAAAAAAAA+AGG - chr2_1:30564190-30564209 None:intergenic 20.0%
!! TTAAACATGATTACATGTAT+CGG + chr2_1:30564434-30564453 Msa0204760:intron 20.0%
!! TAATATGTTGAACTCAAAAT+TGG + chr2_1:30564595-30564614 Msa0204760:intron 20.0%
!!! TTTGAACTTCTAATTTTAAG+TGG + chr2_1:30564741-30564760 Msa0204760:CDS 20.0%
!!! TATTTTACATTTTTTGCTCT+AGG + chr2_1:30564872-30564891 Msa0204760:intron 20.0%
!!! ATTTTACATTTTTTGCTCTA+GGG + chr2_1:30564873-30564892 Msa0204760:intron 20.0%
!! TAACTTTAAATTTATGTGCA+GGG + chr2_1:30564902-30564921 Msa0204760:intron 20.0%
!! AGACTTATATACAAAGATAA+TGG - chr2_1:30565645-30565664 None:intergenic 20.0%
!! AAAAAACTAAGATGTTCTAT+TGG - chr2_1:30565802-30565821 None:intergenic 20.0%
!! TTCATTTGACAAATACATTT+GGG + chr2_1:30566178-30566197 Msa0204760:CDS 20.0%
!!! AATTTGTATTGATTTGAGAT+TGG - chr2_1:30566221-30566240 None:intergenic 20.0%
!! ATCTCAAATCAATACAAATT+TGG + chr2_1:30566221-30566240 Msa0204760:CDS 20.0%
!! AACAAGAAAAATGTTACTAA+TGG + chr2_1:30566659-30566678 Msa0204760:three_prime_UTR 20.0%
!!! AAAGTGTATGAGATTTTTTG+AGG - chr2_1:30563295-30563314 None:intergenic 25.0%
!! GGAGTTTTCATTATGTTTTT+TGG - chr2_1:30563663-30563682 None:intergenic 25.0%
!!! AAGTCAAAGAGTTTTAAAGA+AGG + chr2_1:30563699-30563718 Msa0204760:CDS 25.0%
!!! AGTCAAAGAGTTTTAAAGAA+GGG + chr2_1:30563700-30563719 Msa0204760:CDS 25.0%
! AAAATGAAGATTGCTCATTA+TGG + chr2_1:30563834-30563853 Msa0204760:CDS 25.0%
! TAACTATCAATTACCTGAAT+TGG - chr2_1:30563967-30563986 None:intergenic 25.0%
! TCATGATAAGCAATATAGAT+AGG - chr2_1:30564271-30564290 None:intergenic 25.0%
! TAAACATGATTACATGTATC+GGG + chr2_1:30564435-30564454 Msa0204760:intron 25.0%
! AGTTTATTGAATGGATAGTT+CGG + chr2_1:30564554-30564573 Msa0204760:intron 25.0%
! AAGTGAGATTGACAAAATTA+TGG - chr2_1:30564647-30564666 None:intergenic 25.0%
! ATATTATGTTGTCCATATTC+AGG + chr2_1:30564712-30564731 Msa0204760:intron 25.0%
! CTAACTTTAAATTTATGTGC+AGG + chr2_1:30564901-30564920 Msa0204760:intron 25.0%
! CATTTGTTTATCAGTCAAAT+TGG - chr2_1:30564961-30564980 None:intergenic 25.0%
! ATGTCTATTAGTTCAGAATA+TGG + chr2_1:30564981-30565000 Msa0204760:CDS 25.0%
! ATTGATATAAGCAAAGTTAG+AGG + chr2_1:30565005-30565024 Msa0204760:CDS 25.0%
!! TAATGCTAACCAAATTTTAC+AGG + chr2_1:30565037-30565056 Msa0204760:CDS 25.0%
! AACAGAAACTTATAAGCTAT+TGG - chr2_1:30565254-30565273 None:intergenic 25.0%
! AGTCAAAGGTTTATATACAA+AGG + chr2_1:30565718-30565737 Msa0204760:intron 25.0%
! GGTTTATATACAAAGGTAAA+CGG + chr2_1:30565725-30565744 Msa0204760:intron 25.0%
! GTTCATTTGACAAATACATT+TGG + chr2_1:30566177-30566196 Msa0204760:CDS 25.0%
! TCATTTGACAAATACATTTG+GGG + chr2_1:30566179-30566198 Msa0204760:CDS 25.0%
! TTAAGTCTTGAAAACTTGTA+GGG - chr2_1:30566440-30566459 None:intergenic 25.0%
! TTTAAGTCTTGAAAACTTGT+AGG - chr2_1:30566441-30566460 None:intergenic 25.0%
! TGTTGTGTATAGTATTAGAT+AGG + chr2_1:30566539-30566558 Msa0204760:three_prime_UTR 25.0%
! CAGAAAGAAAAATAACTTTC+AGG - chr2_1:30566565-30566584 None:intergenic 25.0%
!!! AAATGATCTTGATTTTGAGT+CGG - chr2_1:30566797-30566816 None:intergenic 25.0%
! CGATTTGATATCAGATTTAA+CGG + chr2_1:30566927-30566946 Msa0204760:three_prime_UTR 25.0%
! TTCTTTCAAGATTTGGAACA+TGG - chr2_1:30563449-30563468 None:intergenic 30.0%
GAGTTTCTTCTTTCAAGATT+TGG - chr2_1:30563456-30563475 None:intergenic 30.0%
TTTCCTCTCTTTATAGCTAA+TGG - chr2_1:30563597-30563616 None:intergenic 30.0%
AAGAGAGGAAATGACAATAA+TGG + chr2_1:30563606-30563625 Msa0204760:CDS 30.0%
CATAATGAAAACTCCAACTT+TGG + chr2_1:30563668-30563687 Msa0204760:CDS 30.0%
!!! AGTTTTAAAGAAGGGTCTTT+TGG + chr2_1:30563708-30563727 Msa0204760:CDS 30.0%
TTCAAAGACTACTAATCAAG+AGG + chr2_1:30563917-30563936 Msa0204760:CDS 30.0%
TTGCTTATCATGATGAAGAA+TGG + chr2_1:30564278-30564297 Msa0204760:CDS 30.0%
TGCTTATCATGATGAAGAAT+GGG + chr2_1:30564279-30564298 Msa0204760:CDS 30.0%
ACGTAAACTAAGTGTATGTT+TGG + chr2_1:30564528-30564547 Msa0204760:intron 30.0%
GTTTGGATGAGTTTATTGAA+TGG + chr2_1:30564545-30564564 Msa0204760:intron 30.0%
TTGGAACTTTATCAATACTG+CGG + chr2_1:30564614-30564633 Msa0204760:intron 30.0%
TTCAAACAACATCCTGAATA+TGG - chr2_1:30564727-30564746 None:intergenic 30.0%
AGAATTTGATGCAGAAATTG+TGG + chr2_1:30564935-30564954 Msa0204760:CDS 30.0%
ATTGATCAATCAACTCATGT+GGG - chr2_1:30565283-30565302 None:intergenic 30.0%
!! AATATGTGACAGCATTGATA+AGG - chr2_1:30565319-30565338 None:intergenic 30.0%
TTCAGTTGTTTATCAAGATG+CGG - chr2_1:30565400-30565419 None:intergenic 30.0%
ATGTATTTGCAATGAGTCAA+AGG + chr2_1:30565704-30565723 Msa0204760:intron 30.0%
ATATACAAAGGTAAACGGTA+CGG + chr2_1:30565730-30565749 Msa0204760:intron 30.0%
ACATCTTAGTTAGTGATTGT+TGG + chr2_1:30565830-30565849 Msa0204760:intron 30.0%
ATATAAGCTATCCTTACCAA+TGG + chr2_1:30566033-30566052 Msa0204760:intron 30.0%
!! CTTTGAAATTATGCCATTAC+AGG + chr2_1:30566139-30566158 Msa0204760:intron 30.0%
AAGACTTTCTTAACCTGTAA+TGG - chr2_1:30566155-30566174 None:intergenic 30.0%
TTACAGGTTAAGAAAGTCTT+TGG + chr2_1:30566155-30566174 Msa0204760:intron 30.0%
CATTTGACAAATACATTTGG+GGG + chr2_1:30566180-30566199 Msa0204760:CDS 30.0%
ATAAATGAATGAACCACTGT+TGG - chr2_1:30566326-30566345 None:intergenic 30.0%
TGATTAAGTGGTCATTTGTT+AGG - chr2_1:30566361-30566380 None:intergenic 30.0%
GGGTCAATTAATCTCTTATA+TGG - chr2_1:30566420-30566439 None:intergenic 30.0%
!! TGTTACTAATGGTGTTATCA+AGG + chr2_1:30566670-30566689 Msa0204760:three_prime_UTR 30.0%
!! GTTACTAATGGTGTTATCAA+GGG + chr2_1:30566671-30566690 Msa0204760:three_prime_UTR 30.0%
! CAATTTTAAATGATCTCGGA+CGG - chr2_1:30566742-30566761 None:intergenic 30.0%
AAATAATGCAAAGTCCTTGT+AGG - chr2_1:30567107-30567126 None:intergenic 30.0%
TACAAGGACTTTGCATTATT+TGG + chr2_1:30567106-30567125 Msa0204760:three_prime_UTR 30.0%
AAATGAGGGAAATGTTGTGA+GGG - chr2_1:30563235-30563254 None:intergenic 35.0%
AAAATGAGGGAAATGTTGTG+AGG - chr2_1:30563236-30563255 None:intergenic 35.0%
GAAAAGAGAGAGGAAAATGA+GGG - chr2_1:30563249-30563268 None:intergenic 35.0%
TGAAAAGAGAGAGGAAAATG+AGG - chr2_1:30563250-30563269 None:intergenic 35.0%
AGATTGCAATGAAAAGAGAG+AGG - chr2_1:30563259-30563278 None:intergenic 35.0%
TACAGTTACTTCTTTAGCCT+TGG - chr2_1:30563355-30563374 None:intergenic 35.0%
AAGGCTAAAGAAGTAACTGT+AGG + chr2_1:30563354-30563373 Msa0204760:CDS 35.0%
ATGTTGCTAGAATCAATGGA+AGG + chr2_1:30563400-30563419 Msa0204760:CDS 35.0%
!!! GGTAATGGAGAAAGTGATTT+TGG - chr2_1:30563495-30563514 None:intergenic 35.0%
!! GAAGTAGTAGTAGTCTTGTT+TGG - chr2_1:30563522-30563541 None:intergenic 35.0%
!!! TTTGGTTTTGGAGAAATTGG+TGG - chr2_1:30563555-30563574 None:intergenic 35.0%
!!! GACTTTGGTTTTGGAGAAAT+TGG - chr2_1:30563558-30563577 None:intergenic 35.0%
AGACCATTAGCTATAAAGAG+AGG + chr2_1:30563591-30563610 Msa0204760:CDS 35.0%
!! TTCTTTGATCACTGATTCAC+CGG + chr2_1:30563758-30563777 Msa0204760:CDS 35.0%
!! TTTGAATCAAGAGCACTTGA+AGG - chr2_1:30563903-30563922 None:intergenic 35.0%
GCTTATCATGATGAAGAATG+GGG + chr2_1:30564280-30564299 Msa0204760:CDS 35.0%
CACACACACTTATGAAAAAC+TGG + chr2_1:30564351-30564370 Msa0204760:intron 35.0%
ACTTAGTTTACGTGTGTTTG+TGG - chr2_1:30564521-30564540 None:intergenic 35.0%
!! ATTTTAAGTGGTGCTCAAGT+TGG + chr2_1:30564753-30564772 Msa0204760:CDS 35.0%
CATAATCAAACCTGAAGTCT+AGG - chr2_1:30564809-30564828 None:intergenic 35.0%
AATTCACTCGTTGCCAAAAA+AGG + chr2_1:30565211-30565230 Msa0204760:intron 35.0%
TTGATCAATCAACTCATGTG+GGG - chr2_1:30565282-30565301 None:intergenic 35.0%
CATTGATCAATCAACTCATG+TGG - chr2_1:30565284-30565303 None:intergenic 35.0%
AACAACTGAATTGACGCTTA+TGG + chr2_1:30565410-30565429 Msa0204760:intron 35.0%
CTTTCTTTCTCTCTTCTTGT+TGG - chr2_1:30565974-30565993 None:intergenic 35.0%
AAACACTTGTACCATTGGTA+AGG - chr2_1:30566047-30566066 None:intergenic 35.0%
TACCAATGGTACAAGTGTTT+AGG + chr2_1:30566047-30566066 Msa0204760:intron 35.0%
!! TCTGATTTTGATGTCTTCAC+TGG - chr2_1:30566257-30566276 None:intergenic 35.0%
AGCAAAGACATGATTAAGAG+AGG + chr2_1:30566284-30566303 Msa0204760:CDS 35.0%
GCAAAGACATGATTAAGAGA+GGG + chr2_1:30566285-30566304 Msa0204760:CDS 35.0%
AAGAGAGGGTTTAGATATGT+TGG + chr2_1:30566299-30566318 Msa0204760:CDS 35.0%
AGTGGTTCATTCATTTATGC+AGG + chr2_1:30566328-30566347 Msa0204760:CDS 35.0%
!! TGTGCTGACTATAGTTTACT+TGG + chr2_1:30566509-30566528 Msa0204760:three_prime_UTR 35.0%
! ATACTTGTTGAAGAGTGTGT+AGG + chr2_1:30567152-30567171 Msa0204760:three_prime_UTR 35.0%
! TACTTGTTGAAGAGTGTGTA+GGG + chr2_1:30567153-30567172 Msa0204760:three_prime_UTR 35.0%
AAATGTTGTGAGGGATTGGA+GGG - chr2_1:30563226-30563245 None:intergenic 40.0%
ACAAAACATGTGTAGCTCCA+AGG + chr2_1:30563335-30563354 Msa0204760:exon 40.0%
!! CCATTGATTCTAGCAACATG+TGG - chr2_1:30563399-30563418 None:intergenic 40.0%
CCACATGTTGCTAGAATCAA+TGG + chr2_1:30563396-30563415 Msa0204760:CDS 40.0%
! TTGCATGTTGGTTGAAGTAC+TGG - chr2_1:30563426-30563445 None:intergenic 40.0%
! TTTGGAACATGGTTGCATGT+TGG - chr2_1:30563438-30563457 None:intergenic 40.0%
!! GTCTTGTTTGGAAGTGGTAA+TGG - chr2_1:30563510-30563529 None:intergenic 40.0%
!! GTAGTAGTCTTGTTTGGAAG+TGG - chr2_1:30563516-30563535 None:intergenic 40.0%
!! CTAGTAGGTGACTTTGGTTT+TGG - chr2_1:30563567-30563586 None:intergenic 40.0%
CTAGTACTAGTAGGTGACTT+TGG - chr2_1:30563573-30563592 None:intergenic 40.0%
GCTAATGGTCTAGTACTAGT+AGG - chr2_1:30563582-30563601 None:intergenic 40.0%
! TGAAAACTCCAACTTTGGAG+AGG + chr2_1:30563673-30563692 Msa0204760:CDS 40.0%
!! GACTTTTTCCTCTCCAAAGT+TGG - chr2_1:30563684-30563703 None:intergenic 40.0%
!! TCTTTGATCACTGATTCACC+GGG + chr2_1:30563759-30563778 Msa0204760:CDS 40.0%
AAGCATAGCTGCTGTTAGAA+GGG + chr2_1:30563782-30563801 Msa0204760:CDS 40.0%
! AGAGCACTTGAAGGATCAAT+AGG - chr2_1:30563894-30563913 None:intergenic 40.0%
GCTTACTTGTCATCATGAAC+TGG - chr2_1:30564310-30564329 None:intergenic 40.0%
AACACTGCACATGTTAGAGA+CGG + chr2_1:30564466-30564485 Msa0204760:intron 40.0%
! CACGTTTTCGATCATAAGTG+TGG + chr2_1:30564490-30564509 Msa0204760:intron 40.0%
TGGATAGTTCGGCAAAATCA+TGG + chr2_1:30564565-30564584 Msa0204760:intron 40.0%
GAACTTTATCAATACTGCGG+TGG + chr2_1:30564617-30564636 Msa0204760:intron 40.0%
!!! GAAGTCTAGGCGTTTTTTCA+AGG - chr2_1:30564796-30564815 None:intergenic 40.0%
TGAAAAAACGCCTAGACTTC+AGG + chr2_1:30564796-30564815 Msa0204760:CDS 40.0%
!!! AACTTGTCTGCAGCCTTTTT+TGG - chr2_1:30565227-30565246 None:intergenic 40.0%
TAGATATGTTGGTCCAACAG+TGG + chr2_1:30566310-30566329 Msa0204760:CDS 40.0%
GCACTTGCAATGCACATTAT+TGG + chr2_1:30566391-30566410 Msa0204760:CDS 40.0%
TGTGCATTAACTACTCAAGC+AGG + chr2_1:30566704-30566723 Msa0204760:three_prime_UTR 40.0%
!!! TAGAAGCGTGCTTTTTCAAC+AGG + chr2_1:30566970-30566989 Msa0204760:three_prime_UTR 40.0%
AGAATCCGTTAAGAAGTGGT+GGG + chr2_1:30566993-30567012 Msa0204760:three_prime_UTR 40.0%
TTTCCTTTAACAGCCTGTGT+GGG + chr2_1:30567129-30567148 Msa0204760:three_prime_UTR 40.0%
GAAATGTTGTGAGGGATTGG+AGG - chr2_1:30563227-30563246 None:intergenic 45.0%
AGGGAAATGTTGTGAGGGAT+TGG - chr2_1:30563230-30563249 None:intergenic 45.0%
! TCTAGCAACATGTGGTGTTG+TGG - chr2_1:30563391-30563410 None:intergenic 45.0%
GAAGCATAGCTGCTGTTAGA+AGG + chr2_1:30563781-30563800 Msa0204760:CDS 45.0%
TGCTGTTAGAAGGGAACAAG+TGG + chr2_1:30563791-30563810 Msa0204760:CDS 45.0%
AGCTTCATCACAGCCAATTC+AGG + chr2_1:30563951-30563970 Msa0204760:CDS 45.0%
TGTGTGTGTAGAGAGAGAGA+GGG - chr2_1:30564339-30564358 None:intergenic 45.0%
! TATCACCTGTGTCGATGTCT+CGG - chr2_1:30564402-30564421 None:intergenic 45.0%
!! GTGCTCAAGTTGGATCTGAT+TGG + chr2_1:30564763-30564782 Msa0204760:CDS 45.0%
!!! TAGGCGTTTTTTCAAGGTGG+AGG - chr2_1:30564790-30564809 None:intergenic 45.0%
!!! GTCTAGGCGTTTTTTCAAGG+TGG - chr2_1:30564793-30564812 None:intergenic 45.0%
GGCCTAAACACTTGTACCAT+TGG - chr2_1:30566052-30566071 None:intergenic 45.0%
CATTCATTTATGCAGGCAGC+TGG + chr2_1:30566335-30566354 Msa0204760:CDS 45.0%
ACCACTTAATCACTTGCCAC+AGG + chr2_1:30566369-30566388 Msa0204760:CDS 45.0%
CAGGAGAATCCGTTAAGAAG+TGG + chr2_1:30566989-30567008 Msa0204760:three_prime_UTR 45.0%
GAGAATCCGTTAAGAAGTGG+TGG + chr2_1:30566992-30567011 Msa0204760:three_prime_UTR 45.0%
ACACTCCCACCACTTCTTAA+CGG - chr2_1:30567001-30567020 None:intergenic 45.0%
!! TAAGAAGTGGTGGGAGTGTT+AGG + chr2_1:30567002-30567021 Msa0204760:three_prime_UTR 45.0%
!! AAGAAGTGGTGGGAGTGTTA+GGG + chr2_1:30567003-30567022 Msa0204760:three_prime_UTR 45.0%
CAGCATGTGCATGTGCAAAT+AGG + chr2_1:30567032-30567051 Msa0204760:three_prime_UTR 45.0%
CATGTGCATGTGCAAATAGG+AGG + chr2_1:30567035-30567054 Msa0204760:three_prime_UTR 45.0%
CATGTGCAAATAGGAGGCAA+GGG + chr2_1:30567041-30567060 Msa0204760:three_prime_UTR 45.0%
CAAAGTCCTTGTAGGTCACA+TGG - chr2_1:30567099-30567118 None:intergenic 45.0%
GTTTCCTTTAACAGCCTGTG+TGG + chr2_1:30567128-30567147 Msa0204760:three_prime_UTR 45.0%
TATCCCACACAGGCTGTTAA+AGG - chr2_1:30567135-30567154 None:intergenic 45.0%
CTTCAACAAGTATCCCACAC+AGG - chr2_1:30567145-30567164 None:intergenic 45.0%
CTAACAGCAGCTATGCTTCC+CGG - chr2_1:30563780-30563799 None:intergenic 50.0%
TGGCACTTCAGCAAGCACAA+AGG + chr2_1:30563811-30563830 Msa0204760:CDS 50.0%
TGTAGAGAGAGAGAGGGGAA+GGG - chr2_1:30564333-30564352 None:intergenic 50.0%
GTGTGTGTAGAGAGAGAGAG+GGG - chr2_1:30564338-30564357 None:intergenic 50.0%
GTGTGTGTGTAGAGAGAGAG+AGG - chr2_1:30564340-30564359 None:intergenic 50.0%
GCCTGTGGCAAGTGATTAAG+TGG - chr2_1:30566373-30566392 None:intergenic 50.0%
ATGTGCATTGCAAGTGCCTG+TGG - chr2_1:30566388-30566407 None:intergenic 50.0%
GCATGTGCAAATAGGAGGCA+AGG + chr2_1:30567040-30567059 Msa0204760:three_prime_UTR 50.0%
GAGAGACCATGTGACCTACA+AGG + chr2_1:30567090-30567109 Msa0204760:three_prime_UTR 50.0%
GTAGAGAGAGAGAGGGGAAG+GGG - chr2_1:30564332-30564351 None:intergenic 55.0%
GTGTAGAGAGAGAGAGGGGA+AGG - chr2_1:30564334-30564353 None:intergenic 55.0%
CAACACCGAGACATCGACAC+AGG + chr2_1:30564394-30564413 Msa0204760:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 30563223 30567195 30563223 ID=Msa0204760;Name=Msa0204760
chr2_1 mRNA 30563223 30567195 30563223 ID=Msa0204760-mRNA-1;Parent=Msa0204760;Name=Msa0204760-mRNA-1;_AED=0.03;_eAED=0.03;_QI=119|1|1|1|1|1|5|771|390
chr2_1 exon 30563223 30563972 30563223 ID=Msa0204760-mRNA-1:exon:10771;Parent=Msa0204760-mRNA-1
chr2_1 exon 30564266 30564323 30564266 ID=Msa0204760-mRNA-1:exon:10772;Parent=Msa0204760-mRNA-1
chr2_1 exon 30564734 30564817 30564734 ID=Msa0204760-mRNA-1:exon:10773;Parent=Msa0204760-mRNA-1
chr2_1 exon 30564923 30565058 30564923 ID=Msa0204760-mRNA-1:exon:10774;Parent=Msa0204760-mRNA-1
chr2_1 exon 30566161 30567195 30566161 ID=Msa0204760-mRNA-1:exon:10775;Parent=Msa0204760-mRNA-1
chr2_1 five_prime_UTR 30563223 30563341 30563223 ID=Msa0204760-mRNA-1:five_prime_utr;Parent=Msa0204760-mRNA-1
chr2_1 CDS 30563342 30563972 30563342 ID=Msa0204760-mRNA-1:cds;Parent=Msa0204760-mRNA-1
chr2_1 CDS 30564266 30564323 30564266 ID=Msa0204760-mRNA-1:cds;Parent=Msa0204760-mRNA-1
chr2_1 CDS 30564734 30564817 30564734 ID=Msa0204760-mRNA-1:cds;Parent=Msa0204760-mRNA-1
chr2_1 CDS 30564923 30565058 30564923 ID=Msa0204760-mRNA-1:cds;Parent=Msa0204760-mRNA-1
chr2_1 CDS 30566161 30566424 30566161 ID=Msa0204760-mRNA-1:cds;Parent=Msa0204760-mRNA-1
chr2_1 three_prime_UTR 30566425 30567195 30566425 ID=Msa0204760-mRNA-1:three_prime_utr;Parent=Msa0204760-mRNA-1
Gene Sequence

>Msa0204760

ATGTGTAGCTCCAAGGCTAAAGAAGTAACTGTAGGCATAGAATCTTCCACAACACCACATGTTGCTAGAATCAATGGAAGGCCAGTACTTCAACCAACATGCAACCATGTTCCAAATCTTGAAAGAAGAAACTCAATTAAGAAATCAACACCAAAATCACTTTCTCCATTACCACTTCCAAACAAGACTACTACTACTTCTTCATTGACACCACCAATTTCTCCAAAACCAAAGTCACCTACTAGTACTAGACCATTAGCTATAAAGAGAGGAAATGACAATAATGGACTTAATTTAAGCTGTGAAAAGATTTCAATACCAAAAAACATAATGAAAACTCCAACTTTGGAGAGGAAAAAGTCAAAGAGTTTTAAAGAAGGGTCTTTTGGTATTGAAGCTTCATTGAGTTATTCTTCTTCTTTGATCACTGATTCACCGGGAAGCATAGCTGCTGTTAGAAGGGAACAAGTGGCACTTCAGCAAGCACAAAGGAAAATGAAGATTGCTCATTATGGAAGATCAAAGTCTGCTAAATTTGAAAGAGTTTTTCCTATTGATCCTTCAAGTGCTCTTGATTCAAAGACTACTAATCAAGAGGAGAAGAGATGCAGCTTCATCACAGCCAATTCAGATCCTATCTATATTGCTTATCATGATGAAGAATGGGGAGTTCCAGTTCATGATGACAAGATGTTGTTTGAACTTCTAATTTTAAGTGGTGCTCAAGTTGGATCTGATTGGACCTCCACCTTGAAAAAACGCCTAGACTTCAGGGCTGCATTTTCAGAATTTGATGCAGAAATTGTGGCCAATTTGACTGATAAACAAATGATGTCTATTAGTTCAGAATATGGCATTGATATAAGCAAAGTTAGAGGAGTTGTTGATAATGCTAACCAAATTTTACAGGTTAAGAAAGTCTTTGGTTCATTTGACAAATACATTTGGGGGTTTGTGAATCATAAACCAATCTCAAATCAATACAAATTTGGTCACAAGATTCCAGTGAAGACATCAAAATCAGAGAGCATAAGCAAAGACATGATTAAGAGAGGGTTTAGATATGTTGGTCCAACAGTGGTTCATTCATTTATGCAGGCAGCTGGCCTAACAAATGACCACTTAATCACTTGCCACAGGCACTTGCAATGCACATTATTGGCAGCCATATAA
Protein sequence

>Msa0204760

MCSSKAKEVTVGIESSTTPHVARINGRPVLQPTCNHVPNLERRNSIKKSTPKSLSPLPLPNKTTTTSSLTPPISPKPKSPTSTRPLAIKRGNDNNGLNLSCEKISIPKNIMKTPTLERKKSKSFKEGSFGIEASLSYSSSLITDSPGSIAAVRREQVALQQAQRKMKIAHYGRSKSAKFERVFPIDPSSALDSKTTNQEEKRCSFITANSDPIYIAYHDEEWGVPVHDDKMLFELLILSGAQVGSDWTSTLKKRLDFRAAFSEFDAEIVANLTDKQMMSISSEYGIDISKVRGVVDNANQILQVKKVFGSFDKYIWGFVNHKPISNQYKFGHKIPVKTSKSESISKDMIKRGFRYVGPTVVHSFMQAAGLTNDHLITCHRHLQCTLLAAI*