AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0252430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252430 MtrunA17_Chr0c02g0489651 66.447 152 11 1 1 112 2 153 2.77e-60 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0252430 AT1G79200.1 70.270 74 22 0 39 112 85 158 2.03e-34 116

Find 41 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCTCGCACAGCCCATAAA+TGG 0.229703 2_2:-43474835 Msa0252430:CDS
TGTTTGTTTGGATTGAATTC+TGG 0.242449 2_2:-43478648 Msa0252430:five_prime_UTR
TAAAGACACAAAAGGGAAAT+TGG 0.286677 2_2:+43474520 None:intergenic
AACAAAGGGTTTATAGAGAT+TGG 0.299376 2_2:+43474770 None:intergenic
AAGCAGAGCACTTGTTTGTT+TGG 0.309882 2_2:-43478660 Msa0252430:five_prime_UTR
ACATTTGTCTTACATGATAA+AGG 0.311477 2_2:-43474431 Msa0252430:three_prime_UTR
GGTAGCAAACTCATTATTCT+TGG 0.322349 2_2:+43474991 None:intergenic
TTTGTCAAAGCTTGGAATAA+AGG 0.336053 2_2:-43474896 Msa0252430:CDS
AAATAAATTACCTTCAGATT+CGG 0.387909 2_2:+43478575 None:intergenic
GACAAATGTGGCACACATAT+TGG 0.389618 2_2:+43474445 None:intergenic
GTTGTTAGTAACTGTTACAT+AGG 0.419631 2_2:+43474740 None:intergenic
TTGCAGAATTTGTCAAAGCT+TGG 0.436101 2_2:-43474904 Msa0252430:CDS
ATTGAATTCTGGATCGATCA+TGG 0.445411 2_2:-43478637 Msa0252430:exon
GCCTTACCAATTCACACAAC+AGG 0.447747 2_2:+43474483 None:intergenic
AGTGCTCTGCTTGAGGTTGA+AGG 0.455003 2_2:+43478671 None:intergenic
GAAGGTTCAAACAAGTGTTC+CGG 0.465575 2_2:+43478689 None:intergenic
GATTCACATAAAGACACAAA+AGG 0.475162 2_2:+43474512 None:intergenic
AGTGTGCCTGTTGTGTGAAT+TGG 0.482760 2_2:-43474489 Msa0252430:three_prime_UTR
CAGATATGAAGTTAAAAGAC+AGG 0.488328 2_2:-43475824 Msa0252430:CDS
GTATCAATCTTTAAGTGTGT+TGG 0.492368 2_2:-43474656 Msa0252430:three_prime_UTR
TTAAGTGTGTTGGTTGTTCG+CGG 0.503849 2_2:-43474646 Msa0252430:three_prime_UTR
AAACAAGTGTTCCGGTGTCT+AGG 0.509037 2_2:+43478697 None:intergenic
ATTCTTCTCTTCTTTCAGCC+AGG 0.510461 2_2:+43474970 None:intergenic
GCCTGTTGTGTGAATTGGTA+AGG 0.511434 2_2:-43474484 Msa0252430:three_prime_UTR
ACATAGGAAATCTAAACAAA+GGG 0.515262 2_2:+43474756 None:intergenic
CTTGAATCGAATTACTATGA+AGG 0.515517 2_2:-43474869 Msa0252430:CDS
ATTCACATAAAGACACAAAA+GGG 0.525698 2_2:+43474513 None:intergenic
GGTTATGATTTGCAGAGTGA+CGG 0.539060 2_2:-43474552 Msa0252430:three_prime_UTR
TCCGGTGTCTAGGTGACTCT+AGG 0.548135 2_2:+43478707 None:intergenic
AGAATAATGAGTTTGCTACC+TGG 0.553697 2_2:-43474988 Msa0252430:CDS
TTGAATTCTGGATCGATCAT+GGG 0.559938 2_2:-43478636 Msa0252430:exon
ACAAACAAGTGCTCTGCTTG+AGG 0.561088 2_2:+43478664 None:intergenic
TACATAGGAAATCTAAACAA+AGG 0.572493 2_2:+43474755 None:intergenic
TCTTCGCAATCCGAATCTGA+AGG 0.587888 2_2:-43478585 Msa0252430:intron
TTCCATTTATGGGCTGTGCG+AGG 0.589094 2_2:+43474833 None:intergenic
CTTGGCAAAATAGTCATCAC+CGG 0.597107 2_2:+43475009 None:intergenic
CAGCAAAAGTGAAAGAAACA+TGG 0.625791 2_2:-43475787 Msa0252430:intron
GTCTAGGTGACTCTAGGTGT+AGG 0.627436 2_2:+43478713 None:intergenic
ATATTGGTTAATATGTGTAG+TGG 0.632221 2_2:+43474461 None:intergenic
ACCTAGAGTCACCTAGACAC+CGG 0.651741 2_2:-43478708 Msa0252430:five_prime_UTR
TTTATCATGTAAGACAAATG+TGG 0.660289 2_2:+43474433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATAAAAGTAAATAATT+AGG - chr2_2:43474606-43474625 Msa0252430:three_prime_UTR 10.0%
!! ATATATATATAAAAGATTCA+AGG + chr2_2:43475678-43475697 None:intergenic 10.0%
!! AAATATATTTCCAAAATTAA+TGG - chr2_2:43475913-43475932 Msa0252430:intron 10.0%
!! AATATATTTCCAAAATTAAT+GGG - chr2_2:43475914-43475933 Msa0252430:intron 10.0%
!! TTGAAACAAAAAAAAAAAAT+GGG - chr2_2:43478526-43478545 Msa0252430:intron 10.0%
!! TCAAAATAGTGAAATAAAAT+TGG - chr2_2:43474680-43474699 Msa0252430:three_prime_UTR 15.0%
!! CAAAATAGTGAAATAAAATT+GGG - chr2_2:43474681-43474700 Msa0252430:three_prime_UTR 15.0%
!! ATTTCTATAACAAAATCAAA+TGG - chr2_2:43474992-43475011 Msa0252430:CDS 15.0%
!!! TGAATTTCATTTTTGTTAAT+TGG - chr2_2:43475250-43475269 Msa0252430:intron 15.0%
!!! ATTACCTATAAATCATTTTT+AGG - chr2_2:43475812-43475831 Msa0252430:CDS 15.0%
!! TAAACCTAAAAATGATTTAT+AGG + chr2_2:43475819-43475838 None:intergenic 15.0%
!! ATATGTTCTGATAAAAAAAA+GGG + chr2_2:43476608-43476627 None:intergenic 15.0%
!!! CTGAATTTGTTTATATTTTT+AGG - chr2_2:43477543-43477562 Msa0252430:intron 15.0%
!! AAATCCAAACATATTAAAAA+AGG - chr2_2:43477928-43477947 Msa0252430:intron 15.0%
!!! TTTTTTGATTTTCCATTTAT+GGG + chr2_2:43478288-43478307 None:intergenic 15.0%
!!! ATTTTTTGATTTTCCATTTA+TGG + chr2_2:43478289-43478308 None:intergenic 15.0%
!! GTTGAAACAAAAAAAAAAAA+TGG - chr2_2:43478525-43478544 Msa0252430:intron 15.0%
!! TGAAACAAAAAAAAAAAATG+GGG - chr2_2:43478527-43478546 Msa0252430:intron 15.0%
!! AAATAAATTACCTTCAGATT+CGG + chr2_2:43474536-43474555 None:intergenic 20.0%
!! CTAAATTCTAATGTTGATAT+TGG - chr2_2:43474717-43474736 Msa0252430:three_prime_UTR 20.0%
!! TTACCAATTTACAAATATCT+AGG + chr2_2:43475323-43475342 None:intergenic 20.0%
!! TATTCTAAACAAAAGAACAT+TGG - chr2_2:43475447-43475466 Msa0252430:intron 20.0%
!!! AAAATGATTGCTTAATGTTT+TGG + chr2_2:43475759-43475778 None:intergenic 20.0%
!! TTTGATTGGATATAGATTAT+GGG - chr2_2:43476103-43476122 Msa0252430:intron 20.0%
!!! CTACTTTTAAATACATTTTC+AGG + chr2_2:43476329-43476348 None:intergenic 20.0%
!! CTGAAAATGTATTTAAAAGT+AGG - chr2_2:43476327-43476346 Msa0252430:intron 20.0%
!! AAAATGTATTTAAAAGTAGG+AGG - chr2_2:43476330-43476349 Msa0252430:intron 20.0%
!! TTCTATCTTAAAAATTGAGT+TGG + chr2_2:43476399-43476418 None:intergenic 20.0%
!! GATATGTTCTGATAAAAAAA+AGG + chr2_2:43476609-43476628 None:intergenic 20.0%
!! TCATAGAAATGAAGTTATAT+TGG - chr2_2:43477144-43477163 Msa0252430:intron 20.0%
!!! GCTGTTATATTTTCTTTTTA+CGG - chr2_2:43477453-43477472 Msa0252430:intron 20.0%
!!! AGGGAAATTTTTATTAAGTA+AGG - chr2_2:43477691-43477710 Msa0252430:intron 20.0%
!!! TTTTAATATGTTTGGATTTG+CGG + chr2_2:43477927-43477946 None:intergenic 20.0%
!!! TGTTCCTTTTTTAATATGTT+TGG + chr2_2:43477935-43477954 None:intergenic 20.0%
!!! CATATCTTTATTTCAAATGT+AGG - chr2_2:43478061-43478080 Msa0252430:intron 20.0%
!! GAAACAAAAAAAAAAAATGG+GGG - chr2_2:43478528-43478547 Msa0252430:intron 20.0%
! ATTCAATTACACAGAAAAAG+AGG + chr2_2:43474592-43474611 None:intergenic 25.0%
! ATAAATCTGATAAAGGTAGT+AGG - chr2_2:43474885-43474904 Msa0252430:CDS 25.0%
! TAAATTTGATTCAGACTCTT+AGG - chr2_2:43474928-43474947 Msa0252430:CDS 25.0%
!!! AGTAGTTGGTAATGATTAAT+TGG - chr2_2:43475133-43475152 Msa0252430:intron 25.0%
! CAATTTACAAATATCTAGGT+CGG + chr2_2:43475319-43475338 None:intergenic 25.0%
!!! TGTTGTTTTCAATGTGTTTT+AGG - chr2_2:43475371-43475390 Msa0252430:intron 25.0%
!!! TTTTCAATGTGTTTTAGGTT+GGG - chr2_2:43475376-43475395 Msa0252430:intron 25.0%
! TTGAATGTAGAATTCATGTT+TGG - chr2_2:43475646-43475665 Msa0252430:intron 25.0%
! GATATTCTACATTATAGAAC+TGG + chr2_2:43475782-43475801 None:intergenic 25.0%
! TTCTATAATGTAGAATATCC+AGG - chr2_2:43475783-43475802 Msa0252430:intron 25.0%
!! ATACTTTGTCCCATTAATTT+TGG + chr2_2:43475926-43475945 None:intergenic 25.0%
! GTTTGATTGGATATAGATTA+TGG - chr2_2:43476102-43476121 Msa0252430:intron 25.0%
!! GTATCCTTCTTTTTACATAT+CGG - chr2_2:43476352-43476371 Msa0252430:intron 25.0%
! AAAACCGATATGTAAAAAGA+AGG + chr2_2:43476359-43476378 None:intergenic 25.0%
!!! TTTTTACATATCGGTTTTGT+AGG - chr2_2:43476361-43476380 Msa0252430:intron 25.0%
!! ATCAGAACATATCTAAGATA+AGG - chr2_2:43476418-43476437 Msa0252430:intron 25.0%
!! TCAGAACATATCTAAGATAA+GGG - chr2_2:43476419-43476438 Msa0252430:intron 25.0%
! TAAAGTTGTATGTGTTGTAA+GGG - chr2_2:43476460-43476479 Msa0252430:intron 25.0%
! TCCTAATTATAAAGACATCT+CGG + chr2_2:43476743-43476762 None:intergenic 25.0%
!! ACCTTATAAGTCAATTTTGT+AGG - chr2_2:43476850-43476869 Msa0252430:intron 25.0%
!! GACATTTTATAATCATATGC+AGG - chr2_2:43477177-43477196 Msa0252430:intron 25.0%
! GCATTATTAGTAGTAGTATT+GGG + chr2_2:43477364-43477383 None:intergenic 25.0%
! AGCATTATTAGTAGTAGTAT+TGG + chr2_2:43477365-43477384 None:intergenic 25.0%
!! GTGATAAAAGATTTTTCTTC+TGG + chr2_2:43477392-43477411 None:intergenic 25.0%
! AAAATATAACAGCACCAAAA+AGG + chr2_2:43477446-43477465 None:intergenic 25.0%
! TATAAACAAATTCAGCAGAA+AGG + chr2_2:43477538-43477557 None:intergenic 25.0%
!!! AGACTTCTTTTGAAAAATTG+TGG - chr2_2:43477665-43477684 Msa0252430:intron 25.0%
!!! TTTTGAAAAATTGTGGATGA+GGG - chr2_2:43477672-43477691 Msa0252430:intron 25.0%
! ATTAAGTAAGGTGACATTAA+TGG - chr2_2:43477703-43477722 Msa0252430:intron 25.0%
! GACTACTTCATATAAATAAC+AGG + chr2_2:43477989-43478008 None:intergenic 25.0%
!! TTATTTATCTAGTGGTTTGT+TGG - chr2_2:43478029-43478048 Msa0252430:intron 25.0%
! ACATAGGAAATCTAAACAAA+GGG + chr2_2:43478355-43478374 None:intergenic 25.0%
! TACATAGGAAATCTAAACAA+AGG + chr2_2:43478356-43478375 None:intergenic 25.0%
!! CTTTTTACACCTTTTCTTTT+TGG + chr2_2:43478490-43478509 None:intergenic 25.0%
! ATTCACATAAAGACACAAAA+GGG + chr2_2:43478598-43478617 None:intergenic 25.0%
!! ATATTGGTTAATATGTGTAG+TGG + chr2_2:43478650-43478669 None:intergenic 25.0%
! TTTATCATGTAAGACAAATG+TGG + chr2_2:43478678-43478697 None:intergenic 25.0%
! ACATTTGTCTTACATGATAA+AGG - chr2_2:43478677-43478696 Msa0252430:five_prime_UTR 25.0%
TGTTTGTTTGGATTGAATTC+TGG - chr2_2:43474460-43474479 Msa0252430:three_prime_UTR 30.0%
TCCAGAGATAAATCTGATAA+AGG - chr2_2:43474878-43474897 Msa0252430:CDS 30.0%
ACCTTTATCAGATTTATCTC+TGG + chr2_2:43474882-43474901 None:intergenic 30.0%
AATCTTACTTTGTTCCTTTG+CGG - chr2_2:43475175-43475194 Msa0252430:intron 30.0%
CGACCTAGATATTTGTAAAT+TGG - chr2_2:43475317-43475336 Msa0252430:intron 30.0%
!! GTTTTCAATGTGTTTTAGGT+TGG - chr2_2:43475375-43475394 Msa0252430:intron 30.0%
!!! TTTCAATGTGTTTTAGGTTG+GGG - chr2_2:43475377-43475396 Msa0252430:intron 30.0%
AATTCAGTTAAGTAGAGACA+TGG + chr2_2:43475428-43475447 None:intergenic 30.0%
TATCAGTATTCTGTTTGACA+TGG - chr2_2:43475482-43475501 Msa0252430:intron 30.0%
! TAGGAGTTAGAACTTTTGTA+AGG - chr2_2:43475512-43475531 Msa0252430:intron 30.0%
! ATGTCTATTGACTAGATCAT+TGG + chr2_2:43475607-43475626 None:intergenic 30.0%
!!! ATTTTTAGGTTTACTGCTTG+AGG - chr2_2:43475826-43475845 Msa0252430:intron 30.0%
AAGTTGAATTTGCAGTGAAA+TGG + chr2_2:43475982-43476001 None:intergenic 30.0%
AATACACCGATGATTCAAAA+GGG + chr2_2:43476024-43476043 None:intergenic 30.0%
GTAAAGTTGTATGTGTTGTA+AGG - chr2_2:43476459-43476478 Msa0252430:intron 30.0%
CTTACAGTATACTAAATGGT+GGG - chr2_2:43476536-43476555 Msa0252430:intron 30.0%
TCCGAGATGTCTTTATAATT+AGG - chr2_2:43476739-43476758 Msa0252430:intron 30.0%
GAGATGTCTTTATAATTAGG+AGG - chr2_2:43476742-43476761 Msa0252430:intron 30.0%
!! ACCTTATAAGTCGATTTTGT+CGG - chr2_2:43476773-43476792 Msa0252430:intron 30.0%
! GACAAATCGATTTATAAGGT+GGG + chr2_2:43476812-43476831 None:intergenic 30.0%
!! CACCTTATAAATCGATTTGT+CGG - chr2_2:43476811-43476830 Msa0252430:intron 30.0%
!! ACCTTATAAATCGATTTGTC+GGG - chr2_2:43476812-43476831 Msa0252430:intron 30.0%
! GCCTACAAAATTGACTTATA+AGG + chr2_2:43476854-43476873 None:intergenic 30.0%
! CAATTTTGTAGGCTTAAGTT+AGG - chr2_2:43476861-43476880 Msa0252430:intron 30.0%
TATCCCAATCTTAGAAGTTT+GGG + chr2_2:43476892-43476911 None:intergenic 30.0%
ATATCCCAATCTTAGAAGTT+TGG + chr2_2:43476893-43476912 None:intergenic 30.0%
! ACATAAGTCAGATTCTAAGA+TGG + chr2_2:43477017-43477036 None:intergenic 30.0%
! AAGCCTTATAAAGATTACGT+TGG - chr2_2:43477081-43477100 Msa0252430:intron 30.0%
CAGATATGAAGTTAAAAGAC+AGG - chr2_2:43477284-43477303 Msa0252430:intron 30.0%
! ATTTGGTATCAGATCCTTTT+TGG - chr2_2:43477429-43477448 Msa0252430:intron 30.0%
!! CTTTTGAAAAATTGTGGATG+AGG - chr2_2:43477671-43477690 Msa0252430:intron 30.0%
! ATTTTTACAAACCATCCTTG+AGG - chr2_2:43477728-43477747 Msa0252430:intron 30.0%
! TTTTTACAAACCATCCTTGA+GGG - chr2_2:43477729-43477748 Msa0252430:intron 30.0%
CAGCAAGTTTAACAAAATCA+CGG - chr2_2:43477824-43477843 Msa0252430:intron 30.0%
TTGGAGATTTAACAAAACCA+CGG + chr2_2:43477855-43477874 None:intergenic 30.0%
TGTTGAGGTTATTTATCTAG+TGG - chr2_2:43478021-43478040 Msa0252430:intron 30.0%
!! TAGTGGTTTGTTGGTATTTA+TGG - chr2_2:43478038-43478057 Msa0252430:intron 30.0%
!! TAGGATTTTCAAGAATTGTC+CGG - chr2_2:43478080-43478099 Msa0252430:intron 30.0%
! GTTTTTCTCTGATCTGTTAT+CGG - chr2_2:43478163-43478182 Msa0252430:intron 30.0%
TTTGTCAAAGCTTGGAATAA+AGG - chr2_2:43478212-43478231 Msa0252430:intron 30.0%
CTTGAATCGAATTACTATGA+AGG - chr2_2:43478239-43478258 Msa0252430:intron 30.0%
AACAAAGGGTTTATAGAGAT+TGG + chr2_2:43478341-43478360 None:intergenic 30.0%
GTTGTTAGTAACTGTTACAT+AGG + chr2_2:43478371-43478390 None:intergenic 30.0%
GTATCAATCTTTAAGTGTGT+TGG - chr2_2:43478452-43478471 Msa0252430:intron 30.0%
AAAAAAAAAATGGGGGATTC+AGG - chr2_2:43478535-43478554 Msa0252430:intron 30.0%
TAAAGACACAAAAGGGAAAT+TGG + chr2_2:43478591-43478610 None:intergenic 30.0%
GATTCACATAAAGACACAAA+AGG + chr2_2:43478599-43478618 None:intergenic 30.0%
ATTGAATTCTGGATCGATCA+TGG - chr2_2:43474471-43474490 Msa0252430:three_prime_UTR 35.0%
TTGAATTCTGGATCGATCAT+GGG - chr2_2:43474472-43474491 Msa0252430:three_prime_UTR 35.0%
GAAAAAGAGGCAGAATGAAA+AGG + chr2_2:43474579-43474598 None:intergenic 35.0%
TGCAGTGATATTTGATTCAC+TGG - chr2_2:43474653-43474672 Msa0252430:three_prime_UTR 35.0%
GGAAAAGAAAGAGAAACGAA+AGG - chr2_2:43474844-43474863 Msa0252430:CDS 35.0%
CTTTCCAAAGAGTCTCAAAA+GGG - chr2_2:43475102-43475121 Msa0252430:intron 35.0%
ATCTTACTTTGTTCCTTTGC+GGG - chr2_2:43475176-43475195 Msa0252430:intron 35.0%
ATTATTACTAAGTCCCGCAA+AGG + chr2_2:43475192-43475211 None:intergenic 35.0%
ATTAATCATGTATCAGCTGC+AGG - chr2_2:43475210-43475229 Msa0252430:intron 35.0%
! CAAAAGAACATTGGTACTGA+AGG - chr2_2:43475456-43475475 Msa0252430:intron 35.0%
AGTATTCTGTTTGACATGGA+GGG - chr2_2:43475486-43475505 Msa0252430:intron 35.0%
! TAGTTCTTCTAGGAATCAAG+AGG - chr2_2:43475541-43475560 Msa0252430:intron 35.0%
TTTATAGGTAATTGCAAGCC+TGG + chr2_2:43475804-43475823 None:intergenic 35.0%
GAGCAAGGAAAAAACATGAA+AGG - chr2_2:43475853-43475872 Msa0252430:intron 35.0%
! TGGGACAAAGTATCTGTATA+AGG - chr2_2:43475933-43475952 Msa0252430:intron 35.0%
ATGATTCAAAAGGGAAAGAC+AGG + chr2_2:43476015-43476034 None:intergenic 35.0%
! GTCTTTCCCTTTTGAATCAT+CGG - chr2_2:43476015-43476034 Msa0252430:intron 35.0%
CAATACACCGATGATTCAAA+AGG + chr2_2:43476025-43476044 None:intergenic 35.0%
AAACCAATCATTTCACTCCA+TGG - chr2_2:43476058-43476077 Msa0252430:intron 35.0%
! AGCAATCACTAATTTTGCCA+TGG + chr2_2:43476078-43476097 None:intergenic 35.0%
GTGATTGCTTGATGTTTGAT+TGG - chr2_2:43476089-43476108 Msa0252430:intron 35.0%
TTGGATATAGATTATGGGTC+AGG - chr2_2:43476108-43476127 Msa0252430:intron 35.0%
GATAATAGTCCGCTGTTATA+AGG - chr2_2:43476163-43476182 Msa0252430:intron 35.0%
ATTTGCAAGACTACACACAT+AGG - chr2_2:43476254-43476273 Msa0252430:intron 35.0%
AGCCTCTTGTGTAAAAAACT+GGG - chr2_2:43476505-43476524 Msa0252430:intron 35.0%
!!! TACCCAGTTTTTTACACAAG+AGG + chr2_2:43476510-43476529 None:intergenic 35.0%
CTTGTGTAAAAAACTGGGTA+AGG - chr2_2:43476510-43476529 Msa0252430:intron 35.0%
GCTGCTTACAGTATACTAAA+TGG - chr2_2:43476532-43476551 Msa0252430:intron 35.0%
GCTTACAGTATACTAAATGG+TGG - chr2_2:43476535-43476554 Msa0252430:intron 35.0%
TCTGTTATGCTACTCATGAT+CGG - chr2_2:43476634-43476653 Msa0252430:intron 35.0%
GTTCCCAATTCAATGAGATA+TGG - chr2_2:43476716-43476735 Msa0252430:intron 35.0%
GGACCATATCTCATTGAATT+GGG + chr2_2:43476722-43476741 None:intergenic 35.0%
!! CCTTATAAGTCGATTTTGTC+GGG - chr2_2:43476774-43476793 Msa0252430:intron 35.0%
! CGACAAATCGATTTATAAGG+TGG + chr2_2:43476813-43476832 None:intergenic 35.0%
! ACCCGACAAATCGATTTATA+AGG + chr2_2:43476816-43476835 None:intergenic 35.0%
!! TCAACCCAAACTTCTAAGAT+TGG - chr2_2:43476885-43476904 Msa0252430:intron 35.0%
!! CAACCCAAACTTCTAAGATT+GGG - chr2_2:43476886-43476905 Msa0252430:intron 35.0%
! TCTAGGACGTACTATGATAA+AGG - chr2_2:43476939-43476958 Msa0252430:intron 35.0%
! CTCATTGAGCTAACTTTTGA+GGG + chr2_2:43477048-43477067 None:intergenic 35.0%
! ACTCATTGAGCTAACTTTTG+AGG + chr2_2:43477049-43477068 None:intergenic 35.0%
AAAGTTAGCTCAATGAGTGA+AGG - chr2_2:43477051-43477070 Msa0252430:intron 35.0%
TGGCCAACGTAATCTTTATA+AGG + chr2_2:43477087-43477106 None:intergenic 35.0%
CAGCAAAAGTGAAAGAAACA+TGG - chr2_2:43477321-43477340 Msa0252430:intron 35.0%
! TTAGTAGTAGTATTGGGAGT+GGG + chr2_2:43477358-43477377 None:intergenic 35.0%
! ATTAGTAGTAGTATTGGGAG+TGG + chr2_2:43477359-43477378 None:intergenic 35.0%
TTCTTGCCATGCTGAATATT+TGG - chr2_2:43477412-43477431 Msa0252430:intron 35.0%
CTGATACCAAATATTCAGCA+TGG + chr2_2:43477421-43477440 None:intergenic 35.0%
! AACTGACAGTTCAAGAGTAT+AGG - chr2_2:43477627-43477646 Msa0252430:intron 35.0%
! TTTTACAAACCATCCTTGAG+GGG - chr2_2:43477730-43477749 Msa0252430:intron 35.0%
! TTTTCGCAAAAGCTATGCTT+TGG + chr2_2:43477874-43477893 None:intergenic 35.0%
GGTAGCAAACTCATTATTCT+TGG + chr2_2:43478120-43478139 None:intergenic 35.0%
!! AGAATAATGAGTTTGCTACC+TGG - chr2_2:43478120-43478139 Msa0252430:intron 35.0%
TTGCAGAATTTGTCAAAGCT+TGG - chr2_2:43478204-43478223 Msa0252430:intron 35.0%
AAGGTGTAAAAAGCAGAGAA+TGG - chr2_2:43478497-43478516 Msa0252430:intron 35.0%
TGTAAAAAGCAGAGAATGGA+TGG - chr2_2:43478501-43478520 Msa0252430:intron 35.0%
GAAGGTTCAAACAAGTGTTC+CGG + chr2_2:43474422-43474441 None:intergenic 40.0%
! AAGCAGAGCACTTGTTTGTT+TGG - chr2_2:43474448-43474467 Msa0252430:three_prime_UTR 40.0%
CAGATGAGAAGAAAAGCAAG+AGG - chr2_2:43474777-43474796 Msa0252430:three_prime_UTR 40.0%
! ATGATGATGAGAAGAAGAGC+AGG - chr2_2:43474816-43474835 Msa0252430:exon 40.0%
GCTTTCCAAAGAGTCTCAAA+AGG - chr2_2:43475101-43475120 Msa0252430:intron 40.0%
!!! TAAGCCCTTTTGAGACTCTT+TGG + chr2_2:43475109-43475128 None:intergenic 40.0%
AAAGGGCTTATGTCAGTAGT+TGG - chr2_2:43475119-43475138 Msa0252430:intron 40.0%
CAGTATTCTGTTTGACATGG+AGG - chr2_2:43475485-43475504 Msa0252430:intron 40.0%
! AAGGCTATGCTAGTTCTTCT+AGG - chr2_2:43475531-43475550 Msa0252430:intron 40.0%
TCTAGGAATCAAGAGGATAC+TGG - chr2_2:43475548-43475567 Msa0252430:intron 40.0%
CAAAAGGGAAAGACAGGAAT+AGG + chr2_2:43476009-43476028 None:intergenic 40.0%
TTGCCATGGAGTGAAATGAT+TGG + chr2_2:43476064-43476083 None:intergenic 40.0%
TAACCCTAAAAGCAAGCTCA+AGG - chr2_2:43476137-43476156 Msa0252430:intron 40.0%
!! ACTCCTTGAGCTTGCTTTTA+GGG + chr2_2:43476143-43476162 None:intergenic 40.0%
CTTTACATGTCCTATCCTAG+AGG + chr2_2:43476228-43476247 None:intergenic 40.0%
!! CGGTTTTGTAGGATTGAGTT+AGG - chr2_2:43476372-43476391 Msa0252430:intron 40.0%
TTGTATGTGTTGTAAGGGCA+TGG - chr2_2:43476465-43476484 Msa0252430:intron 40.0%
! TGTATGTGTTGTAAGGGCAT+GGG - chr2_2:43476466-43476485 Msa0252430:intron 40.0%
CAGCCTCTTGTGTAAAAAAC+TGG - chr2_2:43476504-43476523 Msa0252430:intron 40.0%
CTGATAAAAAAAAGGGCAGC+CGG + chr2_2:43476601-43476620 None:intergenic 40.0%
CTGTTATGCTACTCATGATC+GGG - chr2_2:43476635-43476654 Msa0252430:intron 40.0%
ATTCTCCAAATGTCCAGTCT+TGG - chr2_2:43476664-43476683 Msa0252430:intron 40.0%
CGGACCATATCTCATTGAAT+TGG + chr2_2:43476723-43476742 None:intergenic 40.0%
! CCCGACAAAATCGACTTATA+AGG + chr2_2:43476777-43476796 None:intergenic 40.0%
! CGATTTTGTCGGGTTAAGTT+AGG - chr2_2:43476784-43476803 Msa0252430:intron 40.0%
! TCGATTTGTCGGGTTAAGTT+AGG - chr2_2:43476822-43476841 Msa0252430:intron 40.0%
AGGACGTACTATGATAAAGG+AGG - chr2_2:43476942-43476961 Msa0252430:intron 40.0%
TCTTACATTGACCAGTGATG+TGG + chr2_2:43477107-43477126 None:intergenic 40.0%
AAAGGCAACACTGGTAAAAC+AGG + chr2_2:43477520-43477539 None:intergenic 40.0%
TCTATGAGATGCTAGCTCAA+TGG + chr2_2:43477769-43477788 None:intergenic 40.0%
TTGAAGACTCCGTGTATGTT+TGG - chr2_2:43477796-43477815 Msa0252430:intron 40.0%
GCTGTGATTCCAAACATACA+CGG + chr2_2:43477808-43477827 None:intergenic 40.0%
CGGTGAGTTTGATAGAATCA+AGG + chr2_2:43477907-43477926 None:intergenic 40.0%
GTCAGAAACTGTGATTGTTG+AGG - chr2_2:43478006-43478025 Msa0252430:intron 40.0%
CTTGGCAAAATAGTCATCAC+CGG + chr2_2:43478102-43478121 None:intergenic 40.0%
ATTCTTCTCTTCTTTCAGCC+AGG + chr2_2:43478141-43478160 None:intergenic 40.0%
!! TTAAGTGTGTTGGTTGTTCG+CGG - chr2_2:43478462-43478481 Msa0252430:intron 40.0%
! GGTTATGATTTGCAGAGTGA+CGG - chr2_2:43478556-43478575 Msa0252430:intron 40.0%
GACAAATGTGGCACACATAT+TGG + chr2_2:43478666-43478685 None:intergenic 40.0%
AAACAAGTGTTCCGGTGTCT+AGG + chr2_2:43474414-43474433 None:intergenic 45.0%
ACAAACAAGTGCTCTGCTTG+AGG + chr2_2:43474447-43474466 None:intergenic 45.0%
TCTTCGCAATCCGAATCTGA+AGG - chr2_2:43474523-43474542 Msa0252430:three_prime_UTR 45.0%
ATGAGAAGAAGAGCAGGAAG+AGG - chr2_2:43474822-43474841 Msa0252430:CDS 45.0%
TGAGAAGAAGAGCAGGAAGA+GGG - chr2_2:43474823-43474842 Msa0252430:CDS 45.0%
TGTTTGACATGGAGGGTCTT+AGG - chr2_2:43475493-43475512 Msa0252430:intron 45.0%
! GAATCAAGAGGATACTGGCT+AGG - chr2_2:43475553-43475572 Msa0252430:intron 45.0%
! TAGAGTAACTGCAGGGGTTT+TGG - chr2_2:43475576-43475595 Msa0252430:intron 45.0%
! AGAGTAACTGCAGGGGTTTT+GGG - chr2_2:43475577-43475596 Msa0252430:intron 45.0%
!!! ACTGCTTGAGGTTTTGAGCA+AGG - chr2_2:43475838-43475857 Msa0252430:intron 45.0%
GAAATGGCACAAGACAAGGA+TGG + chr2_2:43475966-43475985 None:intergenic 45.0%
CAGTGAAATGGCACAAGACA+AGG + chr2_2:43475970-43475989 None:intergenic 45.0%
!! CACTCCTTGAGCTTGCTTTT+AGG + chr2_2:43476144-43476163 None:intergenic 45.0%
CCAAGCAGACCTTATAACAG+CGG + chr2_2:43476175-43476194 None:intergenic 45.0%
ATAGGTTGCCCGACAAATCT+AGG - chr2_2:43476272-43476291 Msa0252430:intron 45.0%
TCTCCACACCTAGATTTGTC+GGG + chr2_2:43476283-43476302 None:intergenic 45.0%
CTCTCCACACCTAGATTTGT+CGG + chr2_2:43476284-43476303 None:intergenic 45.0%
AGGTGTGGAGAGTCTCATAT+TGG - chr2_2:43476292-43476311 Msa0252430:intron 45.0%
! TCTAAGATAAGGGAGAGCCT+TGG - chr2_2:43476429-43476448 Msa0252430:intron 45.0%
ACAACTTTACCATTGCGCCA+AGG + chr2_2:43476449-43476468 None:intergenic 45.0%
ACAAAGCTCCTACATACGCA+GGG + chr2_2:43476575-43476594 None:intergenic 45.0%
! TATGTAGGAGCTTTGTGCAC+CGG - chr2_2:43476579-43476598 Msa0252430:intron 45.0%
AGCTGCACTGAAGGTAATCT+AGG - chr2_2:43476922-43476941 Msa0252430:intron 45.0%
AGGAGGTTACGATTCCGATA+TGG - chr2_2:43476959-43476978 Msa0252430:intron 45.0%
GGTACCAATGTTTGCAGTCT+GGG + chr2_2:43476996-43477015 None:intergenic 45.0%
TGGTACCAATGTTTGCAGTC+TGG + chr2_2:43476997-43477016 None:intergenic 45.0%
GCAACACTGGTAAAACAGGA+TGG + chr2_2:43477516-43477535 None:intergenic 45.0%
ATTCAGCAGAAAGGCAACAC+TGG + chr2_2:43477529-43477548 None:intergenic 45.0%
TGAGATGCTAGCTCAATGGA+CGG + chr2_2:43477765-43477784 None:intergenic 45.0%
AGTGTGCCTGTTGTGTGAAT+TGG - chr2_2:43478619-43478638 Msa0252430:CDS 45.0%
GCCTGTTGTGTGAATTGGTA+AGG - chr2_2:43478624-43478643 Msa0252430:exon 45.0%
GCCTTACCAATTCACACAAC+AGG + chr2_2:43478628-43478647 None:intergenic 45.0%
GTCTAGGTGACTCTAGGTGT+AGG + chr2_2:43474398-43474417 None:intergenic 50.0%
ACCTAGAGTCACCTAGACAC+CGG - chr2_2:43474400-43474419 Msa0252430:three_prime_UTR 50.0%
AGTGCTCTGCTTGAGGTTGA+AGG + chr2_2:43474440-43474459 None:intergenic 50.0%
TGGCTAGGTAGAGTAACTGC+AGG - chr2_2:43475568-43475587 Msa0252430:intron 50.0%
GGCTAGGTAGAGTAACTGCA+GGG - chr2_2:43475569-43475588 Msa0252430:intron 50.0%
GCTAGGTAGAGTAACTGCAG+GGG - chr2_2:43475570-43475589 Msa0252430:intron 50.0%
CCGCTGTTATAAGGTCTGCT+TGG - chr2_2:43476172-43476191 Msa0252430:intron 50.0%
! CAACATGCCATTTCGCCTCT+AGG - chr2_2:43476210-43476229 Msa0252430:intron 50.0%
!! TGCCATTTCGCCTCTAGGAT+AGG - chr2_2:43476215-43476234 Msa0252430:intron 50.0%
GTCCTATCCTAGAGGCGAAA+TGG + chr2_2:43476220-43476239 None:intergenic 50.0%
TTGCCCGACAAATCTAGGTG+TGG - chr2_2:43476277-43476296 Msa0252430:intron 50.0%
CACAAAGCTCCTACATACGC+AGG + chr2_2:43476576-43476595 None:intergenic 50.0%
CACGTCCAAGACTGGACATT+TGG + chr2_2:43476672-43476691 None:intergenic 50.0%
AATGTCCAGTCTTGGACGTG+AGG - chr2_2:43476672-43476691 Msa0252430:intron 50.0%
ATGTCCAGTCTTGGACGTGA+GGG - chr2_2:43476673-43476692 Msa0252430:intron 50.0%
GCTTCTAGTAGCTGCACTGA+AGG - chr2_2:43476913-43476932 Msa0252430:intron 50.0%
GGGAGACTCTGCTTCCATAT+CGG + chr2_2:43476976-43476995 None:intergenic 50.0%
GTCTCCCAGACTGCAAACAT+TGG - chr2_2:43476989-43477008 Msa0252430:intron 50.0%
GATTACGTTGGCCACATCAC+TGG - chr2_2:43477093-43477112 Msa0252430:intron 50.0%
GAACTGTCAGTTGCAGCATG+TGG + chr2_2:43477619-43477638 None:intergenic 50.0%
AGTTCGAATCCCCTCAAGGA+TGG + chr2_2:43477742-43477761 None:intergenic 50.0%
!! ACAAAATCACGGTGTCACCG+TGG - chr2_2:43477835-43477854 Msa0252430:intron 50.0%
! TTCCATTTATGGGCTGTGCG+AGG + chr2_2:43478278-43478297 None:intergenic 50.0%
TTCGCGGCGCCAAAAAGAAA+AGG - chr2_2:43478478-43478497 Msa0252430:intron 50.0%
TCCGGTGTCTAGGTGACTCT+AGG + chr2_2:43474404-43474423 None:intergenic 55.0%
TTGCCAGACCCTGCGTATGT+AGG - chr2_2:43476564-43476583 Msa0252430:intron 55.0%
ACGGAGTTCGAATCCCCTCA+AGG + chr2_2:43477746-43477765 None:intergenic 55.0%
AAGGGAGAGCCTTGGCGCAA+TGG - chr2_2:43476437-43476456 Msa0252430:intron 60.0%
CATACGCAGGGTCTGGCAAG+CGG + chr2_2:43476563-43476582 None:intergenic 60.0%
GCTCCTACATACGCAGGGTC+TGG + chr2_2:43476570-43476589 None:intergenic 60.0%
TCCAGTCTTGGACGTGAGGG+TGG - chr2_2:43476676-43476695 Msa0252430:intron 60.0%
ACCACCCTCACGTCCAAGAC+TGG + chr2_2:43476680-43476699 None:intergenic 60.0%
CGCCTCGCACAGCCCATAAA+TGG - chr2_2:43478273-43478292 Msa0252430:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 43474374 43478756 43474374 ID=Msa0252430;Name=Msa0252430
chr2_2 mRNA 43474374 43478756 43474374 ID=Msa0252430-mRNA-1;Parent=Msa0252430;Name=Msa0252430-mRNA-1;_AED=0.06;_eAED=0.06;_QI=116|0.5|1|1|1|1|3|446|112
chr2_2 exon 43474374 43475047 43474374 ID=Msa0252430-mRNA-1:exon:13474;Parent=Msa0252430-mRNA-1
chr2_2 exon 43475788 43475843 43475788 ID=Msa0252430-mRNA-1:exon:13473;Parent=Msa0252430-mRNA-1
chr2_2 exon 43478586 43478756 43478586 ID=Msa0252430-mRNA-1:exon:13472;Parent=Msa0252430-mRNA-1
chr2_2 five_prime_UTR 43478641 43478756 43478641 ID=Msa0252430-mRNA-1:five_prime_utr;Parent=Msa0252430-mRNA-1
chr2_2 CDS 43478586 43478640 43478586 ID=Msa0252430-mRNA-1:cds;Parent=Msa0252430-mRNA-1
chr2_2 CDS 43475788 43475843 43475788 ID=Msa0252430-mRNA-1:cds;Parent=Msa0252430-mRNA-1
chr2_2 CDS 43474820 43475047 43474820 ID=Msa0252430-mRNA-1:cds;Parent=Msa0252430-mRNA-1
chr2_2 three_prime_UTR 43474374 43474819 43474374 ID=Msa0252430-mRNA-1:three_prime_utr;Parent=Msa0252430-mRNA-1
Gene Sequence

>Msa0252430

ATGGGAAGCGATCGAAAATCAAAGAGAAGTTATTCTTCGCAATCCGAATCTGAAGATATGAAGTTAAAAGACAGGCACAAAAGTAAACGCAGCAAAAGTGAAAGAAACATGGATTTTCAAGAATTGTCCGGTGATGACTATTTTGCCAAGAATAATGAGTTTGCTACCTGGCTGAAAGAAGAGAAGAATGTGTTTTTCTCTGATCTGTTATCGGAGTCAGCACGTGAGTTGTTTGCAGAATTTGTCAAAGCTTGGAATAAAGGAAAGCTTGAATCGAATTACTATGAAGGCATTAAAACTGCGCCTCGCACAGCCCATAAATGGAAAATCAAAAAATAG
Protein sequence

>Msa0252430

MGSDRKSKRSYSSQSESEDMKLKDRHKSKRSKSERNMDFQELSGDDYFAKNNEFATWLKEEKNVFFSDLLSESARELFAEFVKAWNKGKLESNYYEGIKTAPRTAHKWKIKK*