AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0253140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253140 MtrunA17_Chr2g0312051 80.328 61 11 1 1 60 1 61 7.17e-30 105
Msa0253140 MtrunA17_Chr1g0166081 76.562 64 11 2 1 60 1 64 1.60e-28 102
Msa0253140 MtrunA17_Chr5g0417951 75.000 64 12 2 1 60 1 64 5.14e-28 100
Msa0253140 MtrunA17_Chr4g0048281 78.462 65 9 3 1 60 1 65 1.97e-26 96.7
Msa0253140 MtrunA17_Chr2g0312011 58.333 60 20 2 1 59 1 56 1.56e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0253140 AT2G32200.2 48.571 70 29 3 1 67 35 100 2.06e-11 58.9

Find 62 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCAGGGGTGATGGTTTC+TGG 0.152080 2_2:+44759307 Msa0253140:CDS
GGAGGCAGGGAAGAGAATTT+TGG 0.178531 2_2:+44761880 Msa0253140:CDS
CGAATATGTTGCCTCTTTGT+TGG 0.311799 2_2:-44761688 None:intergenic
GTGCCCTTCAGTGCCATCTT+TGG 0.313951 2_2:-44759256 None:intergenic
GCAATGTCAACCACTTCAAT+TGG 0.334034 2_2:+44761857 Msa0253140:CDS
ACTGGTGGTGGATATGATAC+TGG 0.355057 2_2:-44759186 None:intergenic
CATCACCCCTGCTAGTAGTT+TGG 0.398219 2_2:-44759299 None:intergenic
TGTAACTGCACCACCACCTA+TGG 0.404681 2_2:+44759224 Msa0253140:CDS
ATGGTCTTGCTCACATTTGT+TGG 0.406726 2_2:-44761527 None:intergenic
CAATGTCAACCACTTCAATT+GGG 0.406842 2_2:+44761858 Msa0253140:CDS
AAGACCACTAAGGTACTTAC+TGG 0.412831 2_2:+44761739 Msa0253140:CDS
ACCACTTCAATTGGGGAGGC+AGG 0.418972 2_2:+44761866 Msa0253140:CDS
ACATATGGAGCTGTAGATAC+TGG 0.438751 2_2:-44759204 None:intergenic
GGTCTGGAATCCTTTGTTGA+GGG 0.445983 2_2:-44759278 None:intergenic
CCCTGCCTCCCCAATTGAAG+TGG 0.471198 2_2:-44761867 None:intergenic
TAACTTCAACAATAAGCAAC+AGG 0.473699 2_2:+44759161 Msa0253140:CDS
CCCCTGCTAGTAGTTTGGTC+TGG 0.475028 2_2:-44759294 None:intergenic
CTGGTGGTGGATATGATACT+GGG 0.477844 2_2:-44759185 None:intergenic
GGTGGTGGTGCAGTTACATA+TGG 0.482974 2_2:-44759219 None:intergenic
AAGTACCTTAGTGGTCTTGT+GGG 0.487005 2_2:-44761734 None:intergenic
CCTTCCAAAGATGGCACTGA+AGG 0.487973 2_2:+44759252 Msa0253140:CDS
AACACCTCACAAAGCTGAGA+AGG 0.522365 2_2:+44761818 Msa0253140:CDS
TTCCAGACCAAACTACTAGC+AGG 0.528828 2_2:+44759292 Msa0253140:CDS
ATGGGTTATCCTTCCAAAGA+TGG 0.529377 2_2:+44759243 Msa0253140:CDS
ATGACCAGTAAGTACCTTAG+TGG 0.529877 2_2:-44761743 None:intergenic
AGACCATCACAAAGCTACCT+TGG 0.540714 2_2:+44761543 Msa0253140:CDS
CCACTTCAATTGGGGAGGCA+GGG 0.552303 2_2:+44761867 Msa0253140:CDS
CCTTCAGTGCCATCTTTGGA+AGG 0.555857 2_2:-44759252 None:intergenic
AAGCCCTTCTCAGCTTTGTG+AGG 0.570608 2_2:-44761822 None:intergenic
TTCATTGAAATGTGAATCAG+TGG 0.571043 2_2:-44761772 None:intergenic
AGGGGTGATGGTTTCTGGAA+GGG 0.573021 2_2:+44759312 Msa0253140:CDS
TAAGTACCTTAGTGGTCTTG+TGG 0.577787 2_2:-44761735 None:intergenic
TGGTCTGGAATCCTTTGTTG+AGG 0.581792 2_2:-44759279 None:intergenic
CTTGTGGGATGAGTGTAAGT+TGG 0.584418 2_2:-44761719 None:intergenic
CAAACTACTAGCAGGGGTGA+TGG 0.586318 2_2:+44759300 Msa0253140:CDS
TGAGGTGTTGACTGTTGCTG+AGG 0.591242 2_2:-44761804 None:intergenic
TCAACGAAATAATGCTGTGA+TGG 0.592576 2_2:-44761651 None:intergenic
TCCAGACCAAACTACTAGCA+GGG 0.592593 2_2:+44759293 Msa0253140:CDS
GAAGGATAACCCATAGGTGG+TGG 0.596519 2_2:-44759234 None:intergenic
GTAACTGCACCACCACCTAT+GGG 0.597034 2_2:+44759225 Msa0253140:CDS
GTTGAAAAGAAGAAGCTCCA+AGG 0.597425 2_2:-44761560 None:intergenic
CTCATCCCACAAGACCACTA+AGG 0.598318 2_2:+44761729 Msa0253140:CDS
CTTCCAAAGATGGCACTGAA+GGG 0.598808 2_2:+44759253 Msa0253140:CDS
CAGGGGTGATGGTTTCTGGA+AGG 0.601686 2_2:+44759311 Msa0253140:CDS
ACACCTCACAAAGCTGAGAA+GGG 0.602993 2_2:+44761819 Msa0253140:CDS
GAGGTGTTGACTGTTGCTGA+GGG 0.607468 2_2:-44761803 None:intergenic
ACAAAGAGGCAACATATTCG+AGG 0.609785 2_2:+44761691 Msa0253140:CDS
GTCAACCACTTCAATTGGGG+AGG 0.617494 2_2:+44761862 Msa0253140:CDS
GGAGCTGTAGATACTGGTGG+TGG 0.617769 2_2:-44759198 None:intergenic
GCTCCAAGGTAGCTTTGTGA+TGG 0.620669 2_2:-44761546 None:intergenic
TTTGGGTATAGTAAGAACTG+TGG 0.624307 2_2:+44761898 Msa0253140:CDS
CTGAAGGGCACCCTCAACAA+AGG 0.624674 2_2:+44759268 Msa0253140:CDS
TGAAACAATCAATTGAGCGA+TGG 0.629882 2_2:-44761600 None:intergenic
CTCCTGCAAAACCAACAAAG+AGG 0.638872 2_2:+44761677 Msa0253140:CDS
GAGGCAACATATTCGAGGTG+AGG 0.647307 2_2:+44761696 Msa0253140:CDS
TCTTTGGAAGGATAACCCAT+AGG 0.652777 2_2:-44759240 None:intergenic
CCAGACCAAACTACTAGCAG+GGG 0.657589 2_2:+44759294 Msa0253140:CDS
TTGAAATGTGAATCAGTGGA+TGG 0.661193 2_2:-44761768 None:intergenic
TTGGAAGGATAACCCATAGG+TGG 0.675232 2_2:-44759237 None:intergenic
AATGTCAACCACTTCAATTG+GGG 0.677543 2_2:+44761859 Msa0253140:CDS
TGGTGGTGGATATGATACTG+GGG 0.717786 2_2:-44759184 None:intergenic
TATGGAGCTGTAGATACTGG+TGG 0.763094 2_2:-44759201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAAATATTTTATTTT+GGG - chr2_2:44760793-44760812 None:intergenic 0.0%
!!! TTTTTTGTCAATAATATTAT+TGG + chr2_2:44759641-44759660 Msa0253140:intron 10.0%
!! AAATAAAAATAGGTTAAATA+TGG - chr2_2:44760050-44760069 None:intergenic 10.0%
!!! TATTTAATTTTGGAAATTTA+AGG + chr2_2:44760802-44760821 Msa0253140:intron 10.0%
!! TTATGTTGTAAACATATATT+TGG + chr2_2:44759604-44759623 Msa0253140:intron 15.0%
!!! TTTTTTTACAAAAATGAACA+AGG + chr2_2:44761251-44761270 Msa0253140:intron 15.0%
!! TCTATTGAAAAATATGAATT+CGG - chr2_2:44761451-44761470 None:intergenic 15.0%
!!! GAAAAAAAATCTTTAATTTC+TGG + chr2_2:44761469-44761488 Msa0253140:intron 15.0%
!!! GTATTTATTTTTCCATTTGA+TGG + chr2_2:44759527-44759546 Msa0253140:intron 20.0%
!! TTGTCAATAATATTATTGGA+CGG + chr2_2:44759645-44759664 Msa0253140:intron 20.0%
!!! TAAAAATTTCACACGATTTT+GGG - chr2_2:44759758-44759777 None:intergenic 20.0%
!!! ATAAAAATTTCACACGATTT+TGG - chr2_2:44759759-44759778 None:intergenic 20.0%
!! AAAATATAGAGAAAATGGAA+TGG - chr2_2:44760231-44760250 None:intergenic 20.0%
!! GCAAAAAAATATAGAGAAAA+TGG - chr2_2:44760236-44760255 None:intergenic 20.0%
!!! TTTTTTCAAACACTTGTTTA+TGG - chr2_2:44760339-44760358 None:intergenic 20.0%
!! TAAACAAGTGTTTGAAAAAA+TGG + chr2_2:44760339-44760358 Msa0253140:intron 20.0%
!! AAACAAGTGTTTGAAAAAAT+GGG + chr2_2:44760340-44760359 Msa0253140:intron 20.0%
!!! GAATTGATTTTAATTTCCTA+CGG - chr2_2:44760855-44760874 None:intergenic 20.0%
!!! AATCCATCTGAAATATATTT+CGG + chr2_2:44760928-44760947 Msa0253140:intron 20.0%
!!! AAAAAAAAGTCATTTTCGAA+CGG - chr2_2:44761096-44761115 None:intergenic 20.0%
!! TTGCAAAAAGAAGTAATAAA+AGG + chr2_2:44761323-44761342 Msa0253140:intron 20.0%
! AACTTAAAAGCAGATATCTA+GGG - chr2_2:44759367-44759386 None:intergenic 25.0%
! GAATACAATAGGAAAAAACT+GGG - chr2_2:44759451-44759470 None:intergenic 25.0%
! AGAATACAATAGGAAAAAAC+TGG - chr2_2:44759452-44759471 None:intergenic 25.0%
! CAAACTACAAAGAATACAAT+AGG - chr2_2:44759462-44759481 None:intergenic 25.0%
!! TTGTAGTTTGTAGAAGAATA+AGG + chr2_2:44759472-44759491 Msa0253140:intron 25.0%
! AACAAATAACATCCATCAAA+TGG - chr2_2:44759542-44759561 None:intergenic 25.0%
! ACATCAAGTATGATATACAA+GGG - chr2_2:44759566-44759585 None:intergenic 25.0%
! TACATCAAGTATGATATACA+AGG - chr2_2:44759567-44759586 None:intergenic 25.0%
!! ATATTATTGGACGGAATTTT+CGG + chr2_2:44759654-44759673 Msa0253140:intron 25.0%
!! TTTTATGACATGTTCAAACA+TGG + chr2_2:44759773-44759792 Msa0253140:intron 25.0%
! TCAATATTTCGGACAAATTA+GGG - chr2_2:44759932-44759951 None:intergenic 25.0%
! GAATTCACGGAAATAAAAAT+AGG - chr2_2:44760060-44760079 None:intergenic 25.0%
! TTCTTCTTATATGCTTACAA+TGG - chr2_2:44760258-44760277 None:intergenic 25.0%
! CTTGTTTATGGAATTTCAAT+TGG - chr2_2:44760327-44760346 None:intergenic 25.0%
!!! TTTTAATTTCCTACGGTTTT+CGG - chr2_2:44760848-44760867 None:intergenic 25.0%
! ATATTGGACTAAACTTCAAA+CGG - chr2_2:44760888-44760907 None:intergenic 25.0%
! ATTATAATTGCACAGATTCA+AGG + chr2_2:44761220-44761239 Msa0253140:intron 25.0%
!!! TTGATTTAAGTTTTGGAAAC+CGG - chr2_2:44761394-44761413 None:intergenic 25.0%
!!! AGCACTATTGATTTAAGTTT+TGG - chr2_2:44761401-44761420 None:intergenic 25.0%
! TTTCAACTCACTTTCAAAAA+TGG + chr2_2:44761576-44761595 Msa0253140:CDS 25.0%
TAACTTCAACAATAAGCAAC+AGG + chr2_2:44759161-44759180 Msa0253140:CDS 30.0%
GAACTTAAAAGCAGATATCT+AGG - chr2_2:44759368-44759387 None:intergenic 30.0%
AAAACTGGGATAAAAAACTG+TGG - chr2_2:44759437-44759456 None:intergenic 30.0%
! ACGTTTTTGTCTGAATTGTT+CGG + chr2_2:44759683-44759702 Msa0253140:intron 30.0%
!!! GGGACAGATTTGTTTTTTTA+AGG - chr2_2:44759828-44759847 None:intergenic 30.0%
! TCAATATTTCACACGGTTTT+GGG - chr2_2:44759848-44759867 None:intergenic 30.0%
! TGAAATATTGAGACGGATTT+CGG + chr2_2:44759857-44759876 Msa0253140:intron 30.0%
CTCAATATTTCGGACAAATT+AGG - chr2_2:44759933-44759952 None:intergenic 30.0%
ATTTGTCCGAAATATTGAGA+CGG + chr2_2:44759934-44759953 Msa0253140:intron 30.0%
TGAAATCCGTCTCAATATTT+CGG - chr2_2:44759943-44759962 None:intergenic 30.0%
TCAAACTTTCACATGGATTT+GGG - chr2_2:44760014-44760033 None:intergenic 30.0%
ATTTGTCTCAAACTTTCACA+TGG - chr2_2:44760021-44760040 None:intergenic 30.0%
CGTACAAGATTAACACATTA+GGG - chr2_2:44760168-44760187 None:intergenic 30.0%
ATATAGAGAAAATGGAATGG+AGG - chr2_2:44760228-44760247 None:intergenic 30.0%
GGAATTTCAATTGGATAGAA+AGG - chr2_2:44760318-44760337 None:intergenic 30.0%
TAAGGATAGAAAGGAATATG+AGG - chr2_2:44760456-44760475 None:intergenic 30.0%
AAAGCATAAAATAGATCTGG+TGG - chr2_2:44760483-44760502 None:intergenic 30.0%
! GAGAAAGCATAAAATAGATC+TGG - chr2_2:44760486-44760505 None:intergenic 30.0%
! TATTTTATGCTTTCTCTGTC+AGG + chr2_2:44760490-44760509 Msa0253140:intron 30.0%
ACATAAACACAGTATTGTGT+GGG - chr2_2:44760537-44760556 None:intergenic 30.0%
AACATAAACACAGTATTGTG+TGG - chr2_2:44760538-44760557 None:intergenic 30.0%
! ATTTTGTGTGATGTGTGTTT+GGG + chr2_2:44760571-44760590 Msa0253140:intron 30.0%
!! GGGTGTTTTTAATTCAGATT+TGG - chr2_2:44760773-44760792 None:intergenic 30.0%
! TATTTCAGATGGATTTCGTA+CGG - chr2_2:44760923-44760942 None:intergenic 30.0%
! CATCTGAAATATATTTCGGA+CGG + chr2_2:44760932-44760951 Msa0253140:intron 30.0%
AATATATTTCGGACGGAATT+TGG + chr2_2:44760939-44760958 Msa0253140:intron 30.0%
ATATATTTCGGACGGAATTT+GGG + chr2_2:44760940-44760959 Msa0253140:intron 30.0%
TTCTATCACAACATGTCAAA+TGG + chr2_2:44761130-44761149 Msa0253140:intron 30.0%
!! CAAATGGTGGATCAATATAA+TGG + chr2_2:44761146-44761165 Msa0253140:intron 30.0%
!!! CTTTTTGTTGTTTAGTACTC+TGG - chr2_2:44761362-44761381 None:intergenic 30.0%
! CAGAGTACTAAACAACAAAA+AGG + chr2_2:44761360-44761379 Msa0253140:intron 30.0%
CAACAAAAAGGTTATCAAAC+CGG + chr2_2:44761372-44761391 Msa0253140:intron 30.0%
TTCATTGAAATGTGAATCAG+TGG - chr2_2:44761775-44761794 None:intergenic 30.0%
! TTTTGTCTGAATTGTTCGGA+CGG + chr2_2:44759687-44759706 Msa0253140:intron 35.0%
!! AATTTCACACGATTTTGGGA+CGG - chr2_2:44759754-44759773 None:intergenic 35.0%
! TTTTTCCCACAAATTTCGCA+CGG + chr2_2:44759798-44759817 Msa0253140:intron 35.0%
! CTCAATATTTCACACGGTTT+TGG - chr2_2:44759849-44759868 None:intergenic 35.0%
AACCGTGTGAAATATTGAGA+CGG + chr2_2:44759850-44759869 Msa0253140:intron 35.0%
ATCCGTCTCAATATTTCACA+CGG - chr2_2:44759855-44759874 None:intergenic 35.0%
TATTTCGGACAAATTAGGGA+CGG - chr2_2:44759928-44759947 None:intergenic 35.0%
CTCAAACTTTCACATGGATT+TGG - chr2_2:44760015-44760034 None:intergenic 35.0%
GCGTACAAGATTAACACATT+AGG - chr2_2:44760169-44760188 None:intergenic 35.0%
GAAGGTTAGAGAGAAAATGA+AGG - chr2_2:44760194-44760213 None:intergenic 35.0%
ATGGAGGTAGATAGAAATGA+AGG - chr2_2:44760212-44760231 None:intergenic 35.0%
AATAGATCTGGTGGAGAATA+AGG - chr2_2:44760474-44760493 None:intergenic 35.0%
! GATTTTGTGTGATGTGTGTT+TGG + chr2_2:44760570-44760589 Msa0253140:intron 35.0%
! AACTTCAAACGGATTTCAGA+CGG - chr2_2:44760877-44760896 None:intergenic 35.0%
CGTCCGAAATATATTTCAGA+TGG - chr2_2:44760934-44760953 None:intergenic 35.0%
GAATTTACGGAAATTTCCCA+CGG - chr2_2:44761051-44761070 None:intergenic 35.0%
! TTTTCGAACGGAATTTCAGA+CGG - chr2_2:44761084-44761103 None:intergenic 35.0%
TATCACAACATGTCAAATGG+TGG + chr2_2:44761133-44761152 Msa0253140:intron 35.0%
!! ATGGTGGATCAATATAATGG+TGG + chr2_2:44761149-44761168 Msa0253140:intron 35.0%
CGTAGCAGAACAACTAAAAT+AGG + chr2_2:44761278-44761297 Msa0253140:intron 35.0%
AGCAGAACAACTAAAATAGG+CGG + chr2_2:44761281-44761300 Msa0253140:intron 35.0%
!! TTGTTGTTTAGTACTCTGGT+TGG - chr2_2:44761358-44761377 None:intergenic 35.0%
TGAAACAATCAATTGAGCGA+TGG - chr2_2:44761603-44761622 None:intergenic 35.0%
TCAACGAAATAATGCTGTGA+TGG - chr2_2:44761654-44761673 None:intergenic 35.0%
TTGAAATGTGAATCAGTGGA+TGG - chr2_2:44761771-44761790 None:intergenic 35.0%
CAATGTCAACCACTTCAATT+GGG + chr2_2:44761858-44761877 Msa0253140:CDS 35.0%
AATGTCAACCACTTCAATTG+GGG + chr2_2:44761859-44761878 Msa0253140:CDS 35.0%
TTTGGGTATAGTAAGAACTG+TGG + chr2_2:44761898-44761917 Msa0253140:CDS 35.0%
ACATATGGAGCTGTAGATAC+TGG - chr2_2:44759207-44759226 None:intergenic 40.0%
! TCTTTGGAAGGATAACCCAT+AGG - chr2_2:44759243-44759262 None:intergenic 40.0%
! ATGGGTTATCCTTCCAAAGA+TGG + chr2_2:44759243-44759262 Msa0253140:CDS 40.0%
! TATTGGACGGAATTTTCGGA+CGG + chr2_2:44759658-44759677 Msa0253140:intron 40.0%
! TGAATTGTTCGGACGGATTT+GGG + chr2_2:44759694-44759713 Msa0253140:intron 40.0%
!! CGGATTTGGGACAGTTTTAA+TGG + chr2_2:44759707-44759726 Msa0253140:intron 40.0%
GAAATCCGTGCGAAATTTGT+GGG - chr2_2:44759806-44759825 None:intergenic 40.0%
! ATATTGAGACGGATTTCGGA+CGG + chr2_2:44759861-44759880 Msa0253140:intron 40.0%
TCGGACGGCTAATAATTTGA+AGG + chr2_2:44759876-44759895 Msa0253140:intron 40.0%
ACTTTCACATGGATTTGGGA+CGG - chr2_2:44760010-44760029 None:intergenic 40.0%
CAATCGTTTCCCACAGATTT+GGG - chr2_2:44760086-44760105 None:intergenic 40.0%
TCAATCGTTTCCCACAGATT+TGG - chr2_2:44760087-44760106 None:intergenic 40.0%
GGTTAGAGAGAAAATGAAGG+AGG - chr2_2:44760191-44760210 None:intergenic 40.0%
! GAAAGGAAGATGATGATGAG+AGG - chr2_2:44760301-44760320 None:intergenic 40.0%
AGTGAAACACAGTGGTAGAA+AGG - chr2_2:44760384-44760403 None:intergenic 40.0%
TGAAGGCAAATAGCAGAGAA+CGG - chr2_2:44760412-44760431 None:intergenic 40.0%
ACTGAGACTAGATCTAGTGA+AGG - chr2_2:44760429-44760448 None:intergenic 40.0%
GGTGGAGAATAAGGATAGAA+AGG - chr2_2:44760465-44760484 None:intergenic 40.0%
CAGTATTGTGTGGGCAAAAT+AGG - chr2_2:44760528-44760547 None:intergenic 40.0%
! AATTTCCTACGGTTTTCGGA+CGG - chr2_2:44760844-44760863 None:intergenic 40.0%
! ATTTCGTACGGTTTTCACGT+GGG - chr2_2:44760911-44760930 None:intergenic 40.0%
! TTCGGACGAAAATCCGTTTT+CGG - chr2_2:44761026-44761045 None:intergenic 40.0%
!!! AATTTCAGACGGTTTTGGGA+CGG - chr2_2:44761073-44761092 None:intergenic 40.0%
! ACGGAATTTCAGACGGTTTT+GGG - chr2_2:44761077-44761096 None:intergenic 40.0%
! AACGGAATTTCAGACGGTTT+TGG - chr2_2:44761078-44761097 None:intergenic 40.0%
ATAGTGCTAACACTCCCAAT+TGG + chr2_2:44761413-44761432 Msa0253140:intron 40.0%
GGTCAGATACCTAACCAATT+GGG - chr2_2:44761430-44761449 None:intergenic 40.0%
! ATGGTCTTGCTCACATTTGT+TGG - chr2_2:44761530-44761549 None:intergenic 40.0%
GTTGAAAAGAAGAAGCTCCA+AGG - chr2_2:44761563-44761582 None:intergenic 40.0%
CGAATATGTTGCCTCTTTGT+TGG - chr2_2:44761691-44761710 None:intergenic 40.0%
ACAAAGAGGCAACATATTCG+AGG + chr2_2:44761691-44761710 Msa0253140:CDS 40.0%
!! AAGTACCTTAGTGGTCTTGT+GGG - chr2_2:44761737-44761756 None:intergenic 40.0%
! TAAGTACCTTAGTGGTCTTG+TGG - chr2_2:44761738-44761757 None:intergenic 40.0%
! AAGACCACTAAGGTACTTAC+TGG + chr2_2:44761739-44761758 Msa0253140:CDS 40.0%
ATGACCAGTAAGTACCTTAG+TGG - chr2_2:44761746-44761765 None:intergenic 40.0%
GCAATGTCAACCACTTCAAT+TGG + chr2_2:44761857-44761876 Msa0253140:CDS 40.0%
!! TGGTGGTGGATATGATACTG+GGG - chr2_2:44759187-44759206 None:intergenic 45.0%
!! CTGGTGGTGGATATGATACT+GGG - chr2_2:44759188-44759207 None:intergenic 45.0%
!! ACTGGTGGTGGATATGATAC+TGG - chr2_2:44759189-44759208 None:intergenic 45.0%
TATGGAGCTGTAGATACTGG+TGG - chr2_2:44759204-44759223 None:intergenic 45.0%
TTGGAAGGATAACCCATAGG+TGG - chr2_2:44759240-44759259 None:intergenic 45.0%
! CTTCCAAAGATGGCACTGAA+GGG + chr2_2:44759253-44759272 Msa0253140:CDS 45.0%
! GGTCTGGAATCCTTTGTTGA+GGG - chr2_2:44759281-44759300 None:intergenic 45.0%
! TGGTCTGGAATCCTTTGTTG+AGG - chr2_2:44759282-44759301 None:intergenic 45.0%
TTCCAGACCAAACTACTAGC+AGG + chr2_2:44759292-44759311 Msa0253140:CDS 45.0%
TCCAGACCAAACTACTAGCA+GGG + chr2_2:44759293-44759312 Msa0253140:CDS 45.0%
! CTGAATTGTTCGGACGGATT+TGG + chr2_2:44759693-44759712 Msa0253140:intron 45.0%
!!! CACGATTTTGGGACGGAATA+GGG - chr2_2:44759747-44759766 None:intergenic 45.0%
!!! ACACGATTTTGGGACGGAAT+AGG - chr2_2:44759748-44759767 None:intergenic 45.0%
GGAAATCCGTGCGAAATTTG+TGG - chr2_2:44759807-44759826 None:intergenic 45.0%
! TGGATTTGGGACGGAATACA+CGG - chr2_2:44760001-44760020 None:intergenic 45.0%
! CAGATTTGGGACGGAATTCA+CGG - chr2_2:44760073-44760092 None:intergenic 45.0%
TGAATTCCGTCCCAAATCTG+TGG + chr2_2:44760073-44760092 Msa0253140:intron 45.0%
GAATTCCGTCCCAAATCTGT+GGG + chr2_2:44760074-44760093 Msa0253140:intron 45.0%
! TTCCGTCAGTTTTCTTGCTG+TGG + chr2_2:44760596-44760615 Msa0253140:intron 45.0%
!! TCCGTCAGTTTTCTTGCTGT+GGG + chr2_2:44760597-44760616 Msa0253140:intron 45.0%
TCCCACAGCAAGAAAACTGA+CGG - chr2_2:44760601-44760620 None:intergenic 45.0%
! TGTTGTGCTGCTCATCTTAG+TGG + chr2_2:44760632-44760651 Msa0253140:intron 45.0%
TAATTCCGTCCGAAAACCGT+AGG + chr2_2:44760836-44760855 Msa0253140:intron 45.0%
! ACGGTTTTCACGTGGGATAT+TGG - chr2_2:44760904-44760923 None:intergenic 45.0%
! GATTTCGTACGGTTTTCACG+TGG - chr2_2:44760912-44760931 None:intergenic 45.0%
CGAAATTACGATTTGCGACG+CGG + chr2_2:44760964-44760983 Msa0253140:intron 45.0%
! ATCCGTTTTCGGACGGAATT+CGG - chr2_2:44761015-44761034 None:intergenic 45.0%
! GACGAAAATCCGTTTTCGGA+CGG - chr2_2:44761022-44761041 None:intergenic 45.0%
! GATTTTCGTCCGAAATCCGT+GGG + chr2_2:44761032-44761051 Msa0253140:intron 45.0%
CGGAAATTTCCCACGGATTT+CGG - chr2_2:44761044-44761063 None:intergenic 45.0%
!!! CGGTTTTGGGACGGAATTTA+CGG - chr2_2:44761064-44761083 None:intergenic 45.0%
GCTAACACTCCCAATTGGTT+AGG + chr2_2:44761418-44761437 Msa0253140:intron 45.0%
CGGTCAGATACCTAACCAAT+TGG - chr2_2:44761431-44761450 None:intergenic 45.0%
AGACCATCACAAAGCTACCT+TGG + chr2_2:44761543-44761562 Msa0253140:CDS 45.0%
!!! CTCTTTGTTGGTTTTGCAGG+AGG - chr2_2:44761679-44761698 None:intergenic 45.0%
CTCCTGCAAAACCAACAAAG+AGG + chr2_2:44761677-44761696 Msa0253140:CDS 45.0%
!!! TGCCTCTTTGTTGGTTTTGC+AGG - chr2_2:44761682-44761701 None:intergenic 45.0%
CTTGTGGGATGAGTGTAAGT+TGG - chr2_2:44761722-44761741 None:intergenic 45.0%
AACACCTCACAAAGCTGAGA+AGG + chr2_2:44761818-44761837 Msa0253140:CDS 45.0%
ACACCTCACAAAGCTGAGAA+GGG + chr2_2:44761819-44761838 Msa0253140:CDS 45.0%
! GAGGCAGGGAAGAGAATTTT+GGG + chr2_2:44761881-44761900 Msa0253140:CDS 45.0%
!!! TTTTTTTTTTTTTATCTTTT+TGG + chr2_2:44759496-44759515 Msa0253140:intron 5.0%
!!! CAAAATTAAATATTTTATTT+TGG - chr2_2:44760794-44760813 None:intergenic 5.0%
!!! CAAAATAAAATATTTAATTT+TGG + chr2_2:44760792-44760811 Msa0253140:intron 5.0%
! GGTGGTGGTGCAGTTACATA+TGG - chr2_2:44759222-44759241 None:intergenic 50.0%
TGTAACTGCACCACCACCTA+TGG + chr2_2:44759224-44759243 Msa0253140:CDS 50.0%
GTAACTGCACCACCACCTAT+GGG + chr2_2:44759225-44759244 Msa0253140:CDS 50.0%
GAAGGATAACCCATAGGTGG+TGG - chr2_2:44759237-44759256 None:intergenic 50.0%
CCTTCAGTGCCATCTTTGGA+AGG - chr2_2:44759255-44759274 None:intergenic 50.0%
! CCTTCCAAAGATGGCACTGA+AGG + chr2_2:44759252-44759271 Msa0253140:CDS 50.0%
CCAGACCAAACTACTAGCAG+GGG + chr2_2:44759294-44759313 Msa0253140:CDS 50.0%
CATCACCCCTGCTAGTAGTT+TGG - chr2_2:44759302-44759321 None:intergenic 50.0%
CAAACTACTAGCAGGGGTGA+TGG + chr2_2:44759300-44759319 Msa0253140:CDS 50.0%
! AGGGGTGATGGTTTCTGGAA+GGG + chr2_2:44759312-44759331 Msa0253140:CDS 50.0%
CGTTTCCCACAGATTTGGGA+CGG - chr2_2:44760082-44760101 None:intergenic 50.0%
AGGCAAATAGCAGAGAACGG+AGG - chr2_2:44760409-44760428 None:intergenic 50.0%
TGCTGCTCATCTTAGTGGCA+TGG + chr2_2:44760637-44760656 Msa0253140:intron 50.0%
GCTGCTCATCTTAGTGGCAT+GGG + chr2_2:44760638-44760657 Msa0253140:intron 50.0%
! GATTTGCGACGCGGCTTTTT+CGG + chr2_2:44760973-44760992 Msa0253140:intron 50.0%
GGACGGAATTCGGACAGATA+GGG - chr2_2:44761005-44761024 None:intergenic 50.0%
GTCCGAATTCCGTCCGAAAA+CGG + chr2_2:44761010-44761029 Msa0253140:intron 50.0%
! GGATTTTCGTCCGAAATCCG+TGG + chr2_2:44761031-44761050 Msa0253140:intron 50.0%
!! GCTCCAAGGTAGCTTTGTGA+TGG - chr2_2:44761549-44761568 None:intergenic 50.0%
GAGGCAACATATTCGAGGTG+AGG + chr2_2:44761696-44761715 Msa0253140:CDS 50.0%
CTCATCCCACAAGACCACTA+AGG + chr2_2:44761729-44761748 Msa0253140:CDS 50.0%
!! GAGGTGTTGACTGTTGCTGA+GGG - chr2_2:44761806-44761825 None:intergenic 50.0%
!! TGAGGTGTTGACTGTTGCTG+AGG - chr2_2:44761807-44761826 None:intergenic 50.0%
! AAGCCCTTCTCAGCTTTGTG+AGG - chr2_2:44761825-44761844 None:intergenic 50.0%
GTCAACCACTTCAATTGGGG+AGG + chr2_2:44761862-44761881 Msa0253140:CDS 50.0%
! GGAGGCAGGGAAGAGAATTT+TGG + chr2_2:44761880-44761899 Msa0253140:CDS 50.0%
GGAGCTGTAGATACTGGTGG+TGG - chr2_2:44759201-44759220 None:intergenic 55.0%
GTGCCCTTCAGTGCCATCTT+TGG - chr2_2:44759259-44759278 None:intergenic 55.0%
!! CTGAAGGGCACCCTCAACAA+AGG + chr2_2:44759268-44759287 Msa0253140:CDS 55.0%
CCCCTGCTAGTAGTTTGGTC+TGG - chr2_2:44759297-44759316 None:intergenic 55.0%
! CTAGCAGGGGTGATGGTTTC+TGG + chr2_2:44759307-44759326 Msa0253140:CDS 55.0%
! CAGGGGTGATGGTTTCTGGA+AGG + chr2_2:44759311-44759330 Msa0253140:CDS 55.0%
CGGAGGAGAGTGAAACACAG+TGG - chr2_2:44760392-44760411 None:intergenic 55.0%
CGGACGGAATTCGGACAGAT+AGG - chr2_2:44761006-44761025 None:intergenic 55.0%
ACCACTTCAATTGGGGAGGC+AGG + chr2_2:44761866-44761885 Msa0253140:CDS 55.0%
CCACTTCAATTGGGGAGGCA+GGG + chr2_2:44761867-44761886 Msa0253140:CDS 55.0%
CCCTGCCTCCCCAATTGAAG+TGG - chr2_2:44761870-44761889 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 44759156 44761930 44759156 ID=Msa0253140;Name=Msa0253140
chr2_2 mRNA 44759156 44761930 44759156 ID=Msa0253140-mRNA-1;Parent=Msa0253140;Name=Msa0253140-mRNA-1;_AED=0.78;_eAED=0.78;_QI=0|0|0|0.5|1|1|2|0|200
chr2_2 exon 44759156 44759351 44759156 ID=Msa0253140-mRNA-1:exon:13755;Parent=Msa0253140-mRNA-1
chr2_2 exon 44761524 44761930 44761524 ID=Msa0253140-mRNA-1:exon:13756;Parent=Msa0253140-mRNA-1
chr2_2 CDS 44759156 44759351 44759156 ID=Msa0253140-mRNA-1:cds;Parent=Msa0253140-mRNA-1
chr2_2 CDS 44761524 44761930 44761524 ID=Msa0253140-mRNA-1:cds;Parent=Msa0253140-mRNA-1
Gene Sequence

>Msa0253140

ATGAGTAACTTCAACAATAAGCAACAGGCCCCAGTATCATATCCACCACCAGTATCTACAGCTCCATATGTAACTGCACCACCACCTATGGGTTATCCTTCCAAAGATGGCACTGAAGGGCACCCTCAACAAAGGATTCCAGACCAAACTACTAGCAGGGGTGATGGTTTCTGGAAGGGATGTTGTGCTGCTATGTTATCCAACAAATGTGAGCAAGACCATCACAAAGCTACCTTGGAGCTTCTTCTTTTCAACTCACTTTCAAAAATGGACCATCGCTCAATTGATTGTTTCATCGATGAATCACTCTTGAACACAATCATCCATCACAGCATTATTTCGTTGAAGCCTCCTGCAAAACCAACAAAGAGGCAACATATTCGAGGTGAGGCCAACTTACACTCATCCCACAAGACCACTAAGGTACTTACTGGTCATCACCATCCACTGATTCACATTTCAATGAAAAAGTTCTCCCTCAGCAACAGTCAACACCTCACAAAGCTGAGAAGGGCTTTACATGTTTTATGCAATGTCAACCACTTCAATTGGGGAGGCAGGGAAGAGAATTTTGGGTATAGTAAGAACTGTGGAGAAACTTGA
Protein sequence

>Msa0253140

MSNFNNKQQAPVSYPPPVSTAPYVTAPPPMGYPSKDGTEGHPQQRIPDQTTSRGDGFWKGCCAAMLSNKCEQDHHKATLELLLFNSLSKMDHRSIDCFIDESLLNTIIHHSIISLKPPAKPTKRQHIRGEANLHSSHKTTKVLTGHHHPLIHISMKKFSLSNSQHLTKLRRALHVLCNVNHFNWGGREENFGYSKNCGET*