AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0254690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0254690 MtrunA17_Chr2g0315361 93.485 307 17 3 1 305 1 306 0.0 544
Msa0254690 MtrunA17_Chr2g0315371 76.527 311 56 4 1 305 1 300 1.03e-163 457
Msa0254690 MtrunA17_Chr2g0315381 75.082 305 70 5 1 305 1 299 3.52e-151 426
Msa0254690 MtrunA17_Chr7g0244981 37.891 256 126 6 12 267 10 232 3.72e-49 165
Msa0254690 MtrunA17_Chr7g0244971 38.983 236 115 5 12 245 11 219 2.53e-48 163
Msa0254690 MtrunA17_Chr8g0334541 38.655 238 96 7 14 245 4 197 8.86e-41 143
Msa0254690 MtrunA17_Chr8g0350621 37.759 241 110 8 10 245 3 208 1.27e-40 142
Msa0254690 MtrunA17_Chr3g0128671 35.961 203 103 7 62 244 47 242 4.92e-28 110
Msa0254690 MtrunA17_Chr5g0428351 39.831 118 68 2 132 249 167 281 3.55e-23 97.1
Msa0254690 MtrunA17_Chr2g0315401 42.520 127 66 3 178 304 20 139 1.98e-22 91.3
Msa0254690 MtrunA17_Chr7g0275531 45.361 97 44 2 132 219 92 188 4.81e-20 88.6
Msa0254690 MtrunA17_Chr8g0387981 29.384 211 112 7 10 208 6 191 4.93e-19 86.3
Msa0254690 MtrunA17_Chr2g0315341 40.650 123 65 4 185 304 1 118 1.15e-18 80.5
Msa0254690 MtrunA17_Chr8g0350631 52.564 78 32 2 170 245 5 79 2.02e-18 80.5
Msa0254690 MtrunA17_Chr3g0126391 29.954 217 101 8 14 208 5 192 3.02e-18 84.0
Msa0254690 MtrunA17_Chr8g0347251 43.158 95 45 2 125 211 105 198 1.03e-17 82.4
Msa0254690 MtrunA17_Chr7g0224801 50.649 77 34 2 147 223 91 163 4.56e-16 74.7
Msa0254690 MtrunA17_Chr2g0315351 42.241 116 52 6 8 121 6 108 8.07e-16 72.4
Msa0254690 MtrunA17_Chr2g0315391 41.379 116 53 6 8 121 6 108 2.10e-15 71.2
Msa0254690 MtrunA17_Chr7g0248721 40.000 105 51 5 105 206 99 194 7.14e-15 72.4
Msa0254690 MtrunA17_Chr7g0270191 39.773 88 50 1 120 207 116 200 1.37e-14 72.8
Msa0254690 MtrunA17_Chr8g0383351 50.000 60 27 1 147 206 158 214 1.90e-14 71.6
Msa0254690 MtrunA17_Chr1g0194481 40.909 88 49 1 120 207 129 213 2.71e-14 72.4
Msa0254690 MtrunA17_Chr3g0129361 49.180 61 28 1 147 207 142 199 3.87e-14 70.5
Msa0254690 MtrunA17_Chr8g0335811 40.000 90 48 2 120 206 122 208 3.94e-14 70.5
Msa0254690 MtrunA17_Chr5g0431811 33.582 134 74 3 107 235 631 754 1.55e-13 71.2
Msa0254690 MtrunA17_Chr5g0418781 39.326 89 48 2 148 233 123 208 1.81e-13 69.3
Msa0254690 MtrunA17_Chr4g0068541 50.847 59 26 1 148 206 207 262 3.06e-13 69.7
Msa0254690 MtrunA17_Chr5g0443131 52.542 59 25 1 148 206 156 211 3.23e-13 68.9
Msa0254690 MtrunA17_Chr7g0263531 48.387 62 29 1 145 206 357 415 6.80e-13 68.6
Msa0254690 MtrunA17_Chr2g0294231 45.946 74 37 1 148 221 202 272 8.76e-13 68.2
Msa0254690 MtrunA17_Chr5g0418191 50.000 60 27 1 147 206 318 374 1.11e-12 68.2
Msa0254690 MtrunA17_Chr8g0386241 32.394 142 81 5 73 206 23 157 1.15e-12 65.5
Msa0254690 MtrunA17_Chr4g0051881 50.847 59 26 1 148 206 177 232 1.28e-12 67.4
Msa0254690 MtrunA17_Chr7g0218081 48.333 60 28 1 147 206 408 464 1.46e-12 67.8
Msa0254690 MtrunA17_Chr7g0218081 45.902 61 29 2 146 206 225 281 4.09e-11 63.5
Msa0254690 MtrunA17_Chr4g0042831 48.333 60 28 1 147 206 276 332 2.51e-12 67.0
Msa0254690 MtrunA17_Chr1g0147881 34.286 105 62 4 104 206 218 317 3.79e-12 66.2
Msa0254690 MtrunA17_Chr4g0071521 37.349 83 50 1 124 206 220 300 5.51e-12 65.5
Msa0254690 MtrunA17_Chr8g0348311 37.634 93 51 2 121 213 132 217 9.69e-12 64.7
Msa0254690 MtrunA17_Chr3g0101131 46.667 60 29 1 147 206 413 469 1.12e-11 65.5
Msa0254690 MtrunA17_Chr5g0445311 46.667 60 29 1 147 206 362 418 1.20e-11 65.1
Msa0254690 MtrunA17_Chr1g0150801 43.939 66 34 1 142 207 95 157 3.26e-11 60.8
Msa0254690 MtrunA17_Chr5g0432161 48.333 60 28 1 147 206 419 475 4.22e-11 63.5
Msa0254690 MtrunA17_Chr7g0238921 51.667 60 26 1 147 206 122 178 4.50e-11 62.8
Msa0254690 MtrunA17_Chr3g0079691 37.079 89 48 2 147 230 493 578 6.47e-11 63.2
Msa0254690 MtrunA17_Chr1g0184691 38.667 75 44 1 132 206 276 348 7.90e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0254690 AT5G22570.1 33.163 196 105 5 64 259 42 211 8.03e-25 101
Msa0254690 AT3G56400.1 32.020 203 113 4 7 208 1 179 8.08e-25 101
Msa0254690 AT5G01900.1 32.487 197 101 4 13 209 6 170 3.07e-23 96.7
Msa0254690 AT1G66560.1 60.294 68 26 1 149 215 103 170 3.52e-22 93.6
Msa0254690 AT1G66600.1 42.857 112 48 3 148 243 102 213 3.93e-22 93.2
Msa0254690 AT1G66550.2 55.882 68 29 1 149 215 103 170 1.73e-20 89.0
Msa0254690 AT1G66550.1 55.882 68 29 1 149 215 108 175 2.31e-20 88.6
Msa0254690 AT4G11070.1 29.936 157 101 2 61 208 44 200 1.94e-19 87.0
Msa0254690 AT2G46400.1 30.612 196 99 6 13 208 5 163 2.40e-19 86.7
Msa0254690 AT1G80590.1 41.379 87 51 0 128 214 64 150 4.86e-19 84.7
Msa0254690 AT2G40750.1 31.068 206 130 6 13 210 12 213 1.33e-18 85.1
Msa0254690 AT4G11070.2 29.648 199 105 4 10 208 5 168 3.09e-18 83.2
Msa0254690 AT4G23810.1 28.856 201 111 5 89 260 91 288 6.19e-18 83.2
Msa0254690 AT5G24110.1 33.987 153 73 3 63 208 41 172 7.45e-17 79.7
Msa0254690 AT2G40740.1 52.381 63 30 0 149 211 173 235 1.35e-16 79.0
Msa0254690 AT2G40740.3 52.381 63 30 0 149 211 182 244 1.70e-16 78.6
Msa0254690 AT2G46130.1 53.030 66 26 2 142 206 23 84 9.71e-15 69.7
Msa0254690 AT4G39410.1 42.553 94 48 3 125 215 196 286 2.15e-14 72.8
Msa0254690 AT4G18170.1 41.379 87 48 1 120 206 143 226 3.16e-14 72.4
Msa0254690 AT5G49520.1 53.333 60 25 1 148 207 220 276 4.31e-14 72.4
Msa0254690 AT2G44745.1 43.902 82 40 2 128 206 121 199 6.42e-14 70.1
Msa0254690 AT1G64000.1 51.667 60 26 1 147 206 112 168 7.72e-14 69.3
Msa0254690 AT5G41570.1 38.636 88 48 2 122 206 68 152 1.71e-13 68.2
Msa0254690 AT2G37260.1 50.847 59 26 1 148 206 348 403 1.78e-13 70.9
Msa0254690 AT2G37260.2 50.847 59 26 1 148 206 266 321 2.09e-13 70.1
Msa0254690 AT2G37260.3 50.847 59 26 1 148 206 268 323 2.17e-13 70.1
Msa0254690 AT2G47260.1 39.773 88 50 1 120 207 145 229 2.82e-13 69.7
Msa0254690 AT1G29860.1 41.000 100 54 2 109 206 94 190 3.06e-13 69.3
Msa0254690 AT1G69310.4 49.153 59 27 1 148 206 146 201 5.08e-13 68.6
Msa0254690 AT1G69310.2 49.153 59 27 1 148 206 146 201 5.08e-13 68.6
Msa0254690 AT1G69310.1 49.153 59 27 1 148 206 146 201 5.08e-13 68.6
Msa0254690 AT1G13960.1 50.000 60 27 1 147 206 407 463 7.48e-13 69.3
Msa0254690 AT2G03340.1 50.000 60 27 1 147 206 413 469 1.46e-12 68.2
Msa0254690 AT1G13960.2 50.000 60 27 1 147 206 254 310 1.70e-12 67.8
Msa0254690 AT5G46350.1 47.692 65 31 1 142 206 176 237 1.76e-12 67.4
Msa0254690 AT5G13080.1 49.180 61 28 1 147 207 65 122 2.79e-12 63.9
Msa0254690 AT4G26640.7 40.000 100 49 4 147 240 339 433 3.53e-12 67.0
Msa0254690 AT4G26640.1 40.000 100 49 4 147 240 307 401 4.07e-12 67.0
Msa0254690 AT4G26640.2 40.000 100 49 4 147 240 379 473 4.23e-12 67.0
Msa0254690 AT2G04880.2 45.902 61 29 2 146 206 108 164 4.48e-12 66.6
Msa0254690 AT2G04880.2 46.667 60 29 1 147 206 281 337 3.32e-11 63.9
Msa0254690 AT4G26640.5 40.000 100 49 4 147 240 203 297 5.24e-12 66.2
Msa0254690 AT4G26640.6 40.000 100 49 4 147 240 203 297 5.24e-12 66.2
Msa0254690 AT4G26640.4 40.000 100 49 4 147 240 203 297 5.24e-12 66.2
Msa0254690 AT4G26640.3 40.000 100 49 4 147 240 203 297 5.24e-12 66.2
Msa0254690 AT2G04880.1 45.902 61 29 2 146 206 108 164 5.28e-12 66.6
Msa0254690 AT2G04880.1 46.667 60 29 1 147 206 305 361 4.64e-11 63.5
Msa0254690 AT2G23320.1 34.188 117 71 2 94 206 181 295 7.61e-12 65.5
Msa0254690 AT3G01970.1 47.541 61 29 1 147 207 63 120 1.89e-11 61.6
Msa0254690 AT5G26170.1 40.278 72 35 2 146 217 110 173 2.13e-11 62.0
Msa0254690 AT4G26440.1 50.000 58 25 2 149 206 178 231 4.72e-11 63.9
Msa0254690 AT2G30590.1 36.145 83 51 1 124 206 288 368 5.73e-11 63.2
Msa0254690 AT3G01080.2 46.154 65 32 1 142 206 337 398 7.06e-11 63.2
Msa0254690 AT3G01080.1 46.154 65 32 1 142 206 299 360 9.43e-11 62.8
Msa0254690 AT3G62340.1 48.333 60 28 1 148 207 117 173 9.76e-11 61.6

Find 51 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCAGCAACAATATTTA+AGG 0.169221 2_2:-48282595 None:intergenic
CTTTGGCTTCCAACAAATTA+TGG 0.175004 2_2:+48282438 Msa0254690:CDS
TTACCATTCAGACTTAAATT+TGG 0.184409 2_2:+48284825 Msa0254690:CDS
AGATATATACCTGAAATATT+TGG 0.232157 2_2:-48284542 None:intergenic
TAAGATGAAGGAGGAGGATT+TGG 0.258950 2_2:+48282740 Msa0254690:CDS
TCACAAATGCCAAATATTTC+AGG 0.281113 2_2:+48284533 Msa0254690:CDS
TCAAATATTAGAGAAGTTAA+AGG 0.289246 2_2:+48282543 Msa0254690:CDS
AGAGTCATCAGAGGAGAAAA+TGG 0.295638 2_2:-48284851 None:intergenic
AAACATCAAAATCGCATAAC+TGG 0.325641 2_2:-48285035 None:intergenic
ATAACCATCATCAATCAATT+TGG 0.330019 2_2:-48284482 None:intergenic
TCCAACAAATTATGGCAAAA+AGG 0.332872 2_2:+48282446 Msa0254690:CDS
TTGTACCGGACTACATATTA+TGG 0.352265 2_2:+48284781 Msa0254690:CDS
GATTCTCCGGTGAGAACAAT+TGG 0.405307 2_2:-48285011 None:intergenic
TATAGGAACCGCCACATCAT+TGG 0.416491 2_2:-48282682 None:intergenic
TCTTCAGCAACAATATTTAA+GGG 0.422613 2_2:-48282594 None:intergenic
AAGATGAAGGAGGAGGATTT+GGG 0.428960 2_2:+48282741 Msa0254690:CDS
AGTAATCAAGAATATCCGTT+TGG 0.452427 2_2:+48284907 Msa0254690:CDS
ATATAATGGAGAATATGCTT+TGG 0.459020 2_2:+48282421 None:intergenic
CAAATATTAGAGAAGTTAAA+GGG 0.464900 2_2:+48282544 Msa0254690:CDS
ATTCCAAATTTAAGTCTGAA+TGG 0.467467 2_2:-48284828 None:intergenic
GCCTCCAAATTGATTGATGA+TGG 0.475537 2_2:+48284478 Msa0254690:CDS
AACTAATTGTCATAAGATGA+AGG 0.480263 2_2:+48282728 Msa0254690:CDS
CCACTTCTTGCATCTACAAA+CGG 0.482853 2_2:+48284946 Msa0254690:CDS
ATGTATGCAGGAGCTACTAT+AGG 0.489699 2_2:+48284689 Msa0254690:intron
TCATGATGTATCTCTTCCTT+AGG 0.501188 2_2:-48284973 None:intergenic
TCGCATAACTGGAGATTCTC+CGG 0.520000 2_2:-48285024 None:intergenic
AAAAGAACCCTTCGTTGTAC+CGG 0.521119 2_2:+48284767 Msa0254690:CDS
ATCTACAAACGGAGATCCTA+AGG 0.526669 2_2:+48284957 Msa0254690:CDS
TAGGTCAAGTGCACCAACTT+GGG 0.530587 2_2:+48284447 Msa0254690:intron
TTAGGTCAAGTGCACCAACT+TGG 0.536533 2_2:+48284446 Msa0254690:intron
ACCATCATCAATCAATTTGG+AGG 0.542680 2_2:-48284479 None:intergenic
GATGACCATAATATGTAGTC+CGG 0.554702 2_2:-48284786 None:intergenic
GGTTATACATGGAGAAAGTA+CGG 0.556459 2_2:+48284499 Msa0254690:CDS
ATGTAGTCCGGTACAACGAA+GGG 0.569798 2_2:-48284774 None:intergenic
TGATTGATGATGGTTATACA+TGG 0.570958 2_2:+48284488 Msa0254690:CDS
TGCACCAACTTGGGAAAAGA+CGG 0.575025 2_2:+48284456 Msa0254690:CDS
TCAAAACCAATTGTTCTCAC+CGG 0.575943 2_2:+48285005 Msa0254690:CDS
TTGTCATAAGATGAAGGAGG+AGG 0.580122 2_2:+48282734 Msa0254690:CDS
ATGAAGGAGGAGGATTTGGG+TGG 0.585719 2_2:+48282744 Msa0254690:CDS
AATGGTGGTGACTGTCCAAA+CGG 0.615715 2_2:-48284922 None:intergenic
CCGTTTGTAGATGCAAGAAG+TGG 0.617465 2_2:-48284946 None:intergenic
TGCTGAAGATCTTGTGCGTG+AGG 0.635228 2_2:+48282608 Msa0254690:CDS
GCTTATGAGAGGACGTGACA+TGG 0.640238 2_2:+48282482 Msa0254690:CDS
TATGTAGTCCGGTACAACGA+AGG 0.640543 2_2:-48284775 None:intergenic
TGGTAAAGACTCCAATGATG+TGG 0.645157 2_2:+48282671 Msa0254690:CDS
TAATATAGAAGAGTCATCAG+AGG 0.645563 2_2:-48284860 None:intergenic
ATGGAAGATGAGCTTATGAG+AGG 0.648590 2_2:+48282471 Msa0254690:CDS
GTAGATGCAAGAAGTGGCAA+TGG 0.654729 2_2:-48284940 None:intergenic
GATGCAAGAAGTGGCAATGG+TGG 0.680588 2_2:-48284937 None:intergenic
TAAAGACTCCAATGATGTGG+CGG 0.681692 2_2:+48282674 Msa0254690:CDS
TAATTGTCATAAGATGAAGG+AGG 0.758287 2_2:+48282731 Msa0254690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+CGG - chr2_2:48283142-48283161 None:intergenic 0.0%
!! AATATTTATAAAATATTAAT+AGG - chr2_2:48284340-48284359 None:intergenic 0.0%
!!! TTTTCATATTATTTTTTTTG+AGG + chr2_2:48282913-48282932 Msa0254690:intron 10.0%
!!! TTTCATATTATTTTTTTTGA+GGG + chr2_2:48282914-48282933 Msa0254690:intron 10.0%
!!! TATTTTTATAATATCAAACA+AGG + chr2_2:48282957-48282976 Msa0254690:intron 10.0%
!! ATATGAAAATTAATATAGTG+AGG - chr2_2:48282902-48282921 None:intergenic 15.0%
!! ATCAATCTTATAACTTAATT+TGG - chr2_2:48283056-48283075 None:intergenic 15.0%
!! TTAACTTTGTAAGTATAAAA+TGG - chr2_2:48284065-48284084 None:intergenic 15.0%
!!! TATAATTTTTTTTCAGTCTA+GGG - chr2_2:48284173-48284192 None:intergenic 15.0%
!!! ATATAATTTTTTTTCAGTCT+AGG - chr2_2:48284174-48284193 None:intergenic 15.0%
!! TTATATTTACGTAGTATAAT+TGG + chr2_2:48284385-48284404 Msa0254690:intron 15.0%
!! ATAAAATTTAATATGTATGC+AGG + chr2_2:48284677-48284696 Msa0254690:intron 15.0%
!! TCAAATATTAGAGAAGTTAA+AGG + chr2_2:48282543-48282562 Msa0254690:CDS 20.0%
!! CAAATATTAGAGAAGTTAAA+GGG + chr2_2:48282544-48282563 Msa0254690:CDS 20.0%
!! GAAAAATCTTTAACAGTTAT+AGG - chr2_2:48282702-48282721 None:intergenic 20.0%
!!! GTTTAAAACTTTAGAACATT+TGG - chr2_2:48282993-48283012 None:intergenic 20.0%
!! TAAACAAACATTCTAATTTG+AGG - chr2_2:48283547-48283566 None:intergenic 20.0%
!! TCAAATTAGAATGTTTGTTT+AGG + chr2_2:48283546-48283565 Msa0254690:intron 20.0%
!! CAAATTAGAATGTTTGTTTA+GGG + chr2_2:48283547-48283566 Msa0254690:intron 20.0%
!! TAAACAAACATTCTAATTTG+AGG - chr2_2:48283547-48283566 None:intergenic 20.0%
!! TCAAATTAGAATGTTTGTTT+AGG + chr2_2:48283546-48283565 Msa0254690:intron 20.0%
!! CAAATTAGAATGTTTGTTTA+GGG + chr2_2:48283547-48283566 Msa0254690:intron 20.0%
!! AAAATAATTCATGCAATCAT+GGG + chr2_2:48283894-48283913 Msa0254690:intron 20.0%
!! TTGTAAGTATAAAATGGTTT+GGG - chr2_2:48284059-48284078 None:intergenic 20.0%
!! TTTGTAAGTATAAAATGGTT+TGG - chr2_2:48284060-48284079 None:intergenic 20.0%
!!! ATAATTTTTTTTCAGTCTAG+GGG - chr2_2:48284172-48284191 None:intergenic 20.0%
!!! GATATTTTTGTCTTCATATT+AGG + chr2_2:48284428-48284447 Msa0254690:intron 20.0%
!! AGATATATACCTGAAATATT+TGG - chr2_2:48284545-48284564 None:intergenic 20.0%
! TCTTCAGCAACAATATTTAA+GGG - chr2_2:48282597-48282616 None:intergenic 25.0%
! ATCTTCAGCAACAATATTTA+AGG - chr2_2:48282598-48282617 None:intergenic 25.0%
! AACTAATTGTCATAAGATGA+AGG + chr2_2:48282728-48282747 Msa0254690:CDS 25.0%
! TAGGAACATAGTATAAAACA+TGG - chr2_2:48282844-48282863 None:intergenic 25.0%
! GAAACAAGTCAAATACTTAT+AGG - chr2_2:48282863-48282882 None:intergenic 25.0%
!!! AAGTATTTGACTTGTTTCAA+AGG + chr2_2:48282865-48282884 Msa0254690:intron 25.0%
!! TTTTACTTCCATGTTTGTTT+AGG + chr2_2:48283485-48283504 Msa0254690:intron 25.0%
! TTTACTTCCATGTTTGTTTA+GGG + chr2_2:48283486-48283505 Msa0254690:intron 25.0%
!! TTTTACTTCCATGTTTGTTT+AGG + chr2_2:48283485-48283504 Msa0254690:intron 25.0%
! TTTACTTCCATGTTTGTTTA+GGG + chr2_2:48283486-48283505 Msa0254690:intron 25.0%
! GAAAATAATTCATGCAATCA+TGG + chr2_2:48283893-48283912 Msa0254690:intron 25.0%
! TTGAGATTTAATTAGAGCTA+TGG - chr2_2:48284098-48284117 None:intergenic 25.0%
!!! TCGTTAACTTGTCTTTTTAA+GGG - chr2_2:48284209-48284228 None:intergenic 25.0%
!!! TTCGTTAACTTGTCTTTTTA+AGG - chr2_2:48284210-48284229 None:intergenic 25.0%
! ATGATGATCATATATATGTC+TGG - chr2_2:48284294-48284313 None:intergenic 25.0%
! ATATGATCATCATTACTCAA+TGG + chr2_2:48284301-48284320 Msa0254690:intron 25.0%
! ATAACCATCATCAATCAATT+TGG - chr2_2:48284485-48284504 None:intergenic 25.0%
! ATTTGAATGTTACAGATTGA+TGG - chr2_2:48284642-48284661 None:intergenic 25.0%
! TTACCATTCAGACTTAAATT+TGG + chr2_2:48284825-48284844 Msa0254690:CDS 25.0%
! ATTCCAAATTTAAGTCTGAA+TGG - chr2_2:48284831-48284850 None:intergenic 25.0%
TCCAACAAATTATGGCAAAA+AGG + chr2_2:48282446-48282465 Msa0254690:CDS 30.0%
AAATTATGGCAAAAAGGCAA+TGG + chr2_2:48282452-48282471 Msa0254690:CDS 30.0%
TAATTGTCATAAGATGAAGG+AGG + chr2_2:48282731-48282750 Msa0254690:CDS 30.0%
ACAAAAACCCTAAACAAACA+TGG - chr2_2:48283496-48283515 None:intergenic 30.0%
ACAAACATTCTAATTTGAGG+TGG - chr2_2:48283544-48283563 None:intergenic 30.0%
AAGAAGTAAGAAAAACCAGT+TGG - chr2_2:48283719-48283738 None:intergenic 30.0%
! GGTTTTTCTTACTTCTTTGT+CGG + chr2_2:48283722-48283741 Msa0254690:intron 30.0%
! GTTTTTCTTACTTCTTTGTC+GGG + chr2_2:48283723-48283742 Msa0254690:intron 30.0%
ACAAAAACCCTAAACAAACA+TGG - chr2_2:48283496-48283515 None:intergenic 30.0%
ACAAACATTCTAATTTGAGG+TGG - chr2_2:48283544-48283563 None:intergenic 30.0%
TCAACTTTCAATCTCAAGTT+TGG + chr2_2:48284010-48284029 Msa0254690:intron 30.0%
TTGGATAATTCATGTGAGAT+TGG + chr2_2:48284029-48284048 Msa0254690:intron 30.0%
TGGATAATTCATGTGAGATT+GGG + chr2_2:48284030-48284049 Msa0254690:intron 30.0%
ATGATCATATATATGTCTGG+AGG - chr2_2:48284291-48284310 None:intergenic 30.0%
!! TGATTGATGATGGTTATACA+TGG + chr2_2:48284488-48284507 Msa0254690:CDS 30.0%
TCACAAATGCCAAATATTTC+AGG + chr2_2:48284533-48284552 Msa0254690:CDS 30.0%
TAATATAGAAGAGTCATCAG+AGG - chr2_2:48284863-48284882 None:intergenic 30.0%
AGTAATCAAGAATATCCGTT+TGG + chr2_2:48284907-48284926 Msa0254690:CDS 30.0%
AAACATCAAAATCGCATAAC+TGG - chr2_2:48285038-48285057 None:intergenic 30.0%
! CTTTGGCTTCCAACAAATTA+TGG + chr2_2:48282438-48282457 Msa0254690:CDS 35.0%
!! GCCTTTTTGCCATAATTTGT+TGG - chr2_2:48282450-48282469 None:intergenic 35.0%
!!! ACACTTTTGCTGTTGAACAA+TGG + chr2_2:48282651-48282670 Msa0254690:CDS 35.0%
AAGAAGGAAGAAAAACCAGT+TGG - chr2_2:48283448-48283467 None:intergenic 35.0%
! GGTTTTTCTTCCTTCTTTGT+CGG + chr2_2:48283451-48283470 Msa0254690:intron 35.0%
! GTTTTTCTTCCTTCTTTGTC+GGG + chr2_2:48283452-48283471 Msa0254690:intron 35.0%
AAGTAAGAAAAACCAGTTGG+CGG - chr2_2:48283716-48283735 None:intergenic 35.0%
!!! TTTTTTTTCAGTCTAGGGGA+GGG - chr2_2:48284168-48284187 None:intergenic 35.0%
!!! ATTTTTTTTCAGTCTAGGGG+AGG - chr2_2:48284169-48284188 None:intergenic 35.0%
!! TTTTAATTCACAGCACAGCT+AGG + chr2_2:48284265-48284284 Msa0254690:intron 35.0%
ACCATCATCAATCAATTTGG+AGG - chr2_2:48284482-48284501 None:intergenic 35.0%
GGTTATACATGGAGAAAGTA+CGG + chr2_2:48284499-48284518 Msa0254690:CDS 35.0%
TTGTACCGGACTACATATTA+TGG + chr2_2:48284781-48284800 Msa0254690:CDS 35.0%
GATGACCATAATATGTAGTC+CGG - chr2_2:48284789-48284808 None:intergenic 35.0%
TCATGATGTATCTCTTCCTT+AGG - chr2_2:48284976-48284995 None:intergenic 35.0%
TCAAAACCAATTGTTCTCAC+CGG + chr2_2:48285005-48285024 Msa0254690:CDS 35.0%
ATGGAAGATGAGCTTATGAG+AGG + chr2_2:48282471-48282490 Msa0254690:CDS 40.0%
TGGTAAAGACTCCAATGATG+TGG + chr2_2:48282671-48282690 Msa0254690:CDS 40.0%
TAAAGACTCCAATGATGTGG+CGG + chr2_2:48282674-48282693 Msa0254690:CDS 40.0%
TTGTCATAAGATGAAGGAGG+AGG + chr2_2:48282734-48282753 Msa0254690:CDS 40.0%
TAAGATGAAGGAGGAGGATT+TGG + chr2_2:48282740-48282759 Msa0254690:CDS 40.0%
AAGATGAAGGAGGAGGATTT+GGG + chr2_2:48282741-48282760 Msa0254690:CDS 40.0%
!!! GTTCGACACTTTCGAGTTTT+TGG - chr2_2:48282799-48282818 None:intergenic 40.0%
AAGGAAGAAAAACCAGTTGG+CGG - chr2_2:48283445-48283464 None:intergenic 40.0%
GACAATTTACCCGACAAAGA+AGG - chr2_2:48283464-48283483 None:intergenic 40.0%
!! GTGGTTTTTGTTTCTCTCTC+TGG - chr2_2:48283525-48283544 None:intergenic 40.0%
!! GTGGTTTTTGTTTCTCTCTC+TGG - chr2_2:48283525-48283544 None:intergenic 40.0%
!!! TTTTTTTCAGTCTAGGGGAG+GGG - chr2_2:48284167-48284186 None:intergenic 40.0%
GCCTCCAAATTGATTGATGA+TGG + chr2_2:48284478-48284497 Msa0254690:CDS 40.0%
ATGTATGCAGGAGCTACTAT+AGG + chr2_2:48284689-48284708 Msa0254690:intron 40.0%
AAAAGAACCCTTCGTTGTAC+CGG + chr2_2:48284767-48284786 Msa0254690:CDS 40.0%
AGAGTCATCAGAGGAGAAAA+TGG - chr2_2:48284854-48284873 None:intergenic 40.0%
CCACTTCTTGCATCTACAAA+CGG + chr2_2:48284946-48284965 Msa0254690:CDS 40.0%
ATCTACAAACGGAGATCCTA+AGG + chr2_2:48284957-48284976 Msa0254690:CDS 40.0%
!!! GGTGAGAACAATTGGTTTTG+AGG - chr2_2:48285006-48285025 None:intergenic 40.0%
TATAGGAACCGCCACATCAT+TGG - chr2_2:48282685-48282704 None:intergenic 45.0%
GAGGAGAGGGAGAAAAATGA+AGG - chr2_2:48283423-48283442 None:intergenic 45.0%
GAGGAGAGGGAGAAAAATGA+AGG - chr2_2:48283423-48283442 None:intergenic 45.0%
TAAGAAAAACCAGTTGGCGG+AGG - chr2_2:48283713-48283732 None:intergenic 45.0%
!!! TTTTTTCAGTCTAGGGGAGG+GGG - chr2_2:48284166-48284185 None:intergenic 45.0%
TTAGGTCAAGTGCACCAACT+TGG + chr2_2:48284446-48284465 Msa0254690:intron 45.0%
TAGGTCAAGTGCACCAACTT+GGG + chr2_2:48284447-48284466 Msa0254690:intron 45.0%
TGCACCAACTTGGGAAAAGA+CGG + chr2_2:48284456-48284475 Msa0254690:CDS 45.0%
ATGTAGTCCGGTACAACGAA+GGG - chr2_2:48284777-48284796 None:intergenic 45.0%
TATGTAGTCCGGTACAACGA+AGG - chr2_2:48284778-48284797 None:intergenic 45.0%
! AATGGTGGTGACTGTCCAAA+CGG - chr2_2:48284925-48284944 None:intergenic 45.0%
GTAGATGCAAGAAGTGGCAA+TGG - chr2_2:48284943-48284962 None:intergenic 45.0%
CCGTTTGTAGATGCAAGAAG+TGG - chr2_2:48284949-48284968 None:intergenic 45.0%
GATTCTCCGGTGAGAACAAT+TGG - chr2_2:48285014-48285033 None:intergenic 45.0%
TCGCATAACTGGAGATTCTC+CGG - chr2_2:48285027-48285046 None:intergenic 45.0%
!!! GAATTTATTTAATATTTTAT+AGG - chr2_2:48284128-48284147 None:intergenic 5.0%
GCTTATGAGAGGACGTGACA+TGG + chr2_2:48282482-48282501 Msa0254690:CDS 50.0%
TGCTGAAGATCTTGTGCGTG+AGG + chr2_2:48282608-48282627 Msa0254690:CDS 50.0%
ATGAAGGAGGAGGATTTGGG+TGG + chr2_2:48282744-48282763 Msa0254690:CDS 50.0%
GAAGAAAAACCAGTTGGCGG+AGG - chr2_2:48283442-48283461 None:intergenic 50.0%
GATGCAAGAAGTGGCAATGG+TGG - chr2_2:48284940-48284959 None:intergenic 50.0%
AAACCAGTTGGCGGAGGAGA+GGG - chr2_2:48283436-48283455 None:intergenic 55.0%
AAAACCAGTTGGCGGAGGAG+AGG - chr2_2:48283437-48283456 None:intergenic 55.0%
AAACCAGTTGGCGGAGGAGA+GGG - chr2_2:48283436-48283455 None:intergenic 55.0%
AAAACCAGTTGGCGGAGGAG+AGG - chr2_2:48283437-48283456 None:intergenic 55.0%
! GAGGCCGTCTTTTCCCAAGT+TGG - chr2_2:48284463-48284482 None:intergenic 55.0%
CAGTCTAGGGGAGGGGGATT+TGG - chr2_2:48284160-48284179 None:intergenic 60.0%
TCTCCCTCTCCTCCGCCAAC+TGG + chr2_2:48283430-48283449 Msa0254690:intron 65.0%
TCTCCCTCTCCTCCGCCAAC+TGG + chr2_2:48283430-48283449 Msa0254690:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 48282426 48285110 48282426 ID=Msa0254690;Name=Msa0254690
chr2_2 mRNA 48282426 48285110 48282426 ID=Msa0254690-mRNA-1;Parent=Msa0254690;Name=Msa0254690-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|3|0|305
chr2_2 exon 48282426 48282826 48282426 ID=Msa0254690-mRNA-1:exon:14569;Parent=Msa0254690-mRNA-1
chr2_2 exon 48284450 48284554 48284450 ID=Msa0254690-mRNA-1:exon:14570;Parent=Msa0254690-mRNA-1
chr2_2 exon 48284699 48285110 48284699 ID=Msa0254690-mRNA-1:exon:14571;Parent=Msa0254690-mRNA-1
chr2_2 CDS 48282426 48282826 48282426 ID=Msa0254690-mRNA-1:cds;Parent=Msa0254690-mRNA-1
chr2_2 CDS 48284450 48284554 48284450 ID=Msa0254690-mRNA-1:cds;Parent=Msa0254690-mRNA-1
chr2_2 CDS 48284699 48285110 48284699 ID=Msa0254690-mRNA-1:cds;Parent=Msa0254690-mRNA-1
Gene Sequence

>Msa0254690

ATGGAGAATATGCTTTGGCTTCCAACAAATTATGGCAAAAAGGCAATGGAAGATGAGCTTATGAGAGGACGTGACATGGCAAATCAATTACTTGAAGTACTAACTTTTGATGATAAGTCAAATATTAGAGAAGTTAAAGGGTCAAACACAAATTCATCAAAAGTGTTACCCTTAAATATTGTTGCTGAAGATCTTGTGCGTGAGGTGCTCAAATCATTGACAAACACACTTTTGCTGTTGAACAATGGTAAAGACTCCAATGATGTGGCGGTTCCTATAACTGTTAAAGATTTTTCTTTTTCAACTAATTGTCATAAGATGAAGGAGGAGGATTTGGGTGGAGCTTGCAAGAAACTCAAAACTCTCAACACCAAAAACTCGAAAGTGTCGAACAAGAGAAAGTCAAGTGCACCAACTTGGGAAAAGACGGCCTCCAAATTGATTGATGATGGTTATACATGGAGAAAGTACGGTCAAAAAATGATCACAAATGCCAAATATTTCAGGAGCTACTATAGGTGCACTCACATGAATGATCAACAATGTGAAGCAATCAAACATGTTCAAAGAATACAAAAGAACCCTTCGTTGTACCGGACTACATATTATGGTCATCACACTTGCAAAAGCTCTTACCATTCAGACTTAAATTTGGAATCCATTTTCTCCTCTGATGACTCTTCTATATTACTTAGTTTCGATAACAACATTTCAAGTAATCAAGAATATCCGTTTGGACAGTCACCACCATTGCCACTTCTTGCATCTACAAACGGAGATCCTAAGGAAGAGATACATCATGATCATTTTCCTCAAAACCAATTGTTCTCACCGGAGAATCTCCAGTTATGCGATTTTGATGTTTATTTTGATTATTTGAGACATGTCACTGAGTCATCATCGAGTGAATCTTTTTAG
Protein sequence

>Msa0254690

MENMLWLPTNYGKKAMEDELMRGRDMANQLLEVLTFDDKSNIREVKGSNTNSSKVLPLNIVAEDLVREVLKSLTNTLLLLNNGKDSNDVAVPITVKDFSFSTNCHKMKEEDLGGACKKLKTLNTKNSKVSNKRKSSAPTWEKTASKLIDDGYTWRKYGQKMITNAKYFRSYYRCTHMNDQQCEAIKHVQRIQKNPSLYRTTYYGHHTCKSSYHSDLNLESIFSSDDSSILLSFDNNISSNQEYPFGQSPPLPLLASTNGDPKEEIHHDHFPQNQLFSPENLQLCDFDVYFDYLRHVTESSSSESF*