AlfalfaGEDB | Msa0258500

M. sativa cultivar zhongmu-4 / Msa0258500

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258500	AES67507.1	82.511	223	30	3	2	215	1	223	4.09e-119	369

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258500	sp\|Q9SKN5\|ARFJ_ARATH	69.714	175	34	6	58	215	2	174	8.34e-70	231

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258500	G7IRU1	82.511	223	30	3	2	215	1	223	1.95e-119	369

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0258500	Msa0824730	0.808511	2.987285e-50	-8.615850e-47
Msa0258500	Msa0863800	0.801194	1.010015e-48	-8.615850e-47
Msa0258500	Msa0993530	0.804450	2.147524e-49	-8.615850e-47
Msa0258500	Msa1398860	0.815061	1.120881e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258500	MtrunA17_Chr2g0326761	91.018	167	8	2	56	215	1	167	6.75e-101	310
Msa0258500	MtrunA17_Chr1g0199681	66.864	169	45	4	57	215	85	252	2.70e-71	232
Msa0258500	MtrunA17_Chr4g0028971	72.353	170	35	5	56	215	1	168	1.08e-70	231
Msa0258500	MtrunA17_Chr1g0181211	62.209	172	51	4	57	215	18	188	1.25e-67	223
Msa0258500	MtrunA17_Chr7g0264171	56.322	174	66	3	45	211	13	183	1.78e-59	199
Msa0258500	MtrunA17_Chr7g0264161	55.944	143	56	2	75	210	1	143	8.33e-49	164
Msa0258500	MtrunA17_Chr3g0115271	47.399	173	83	3	51	215	4	176	1.12e-41	151
Msa0258500	MtrunA17_Chr2g0285461	36.792	212	101	6	55	233	11	222	5.61e-34	130
Msa0258500	MtrunA17_Chr1g0156321	38.739	222	109	8	53	253	27	242	1.79e-33	129
Msa0258500	MtrunA17_Chr2g0277461	41.935	155	76	4	65	207	12	164	2.83e-33	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258500	AT2G28350.2	69.714	175	34	6	58	215	2	174	8.15e-71	231
Msa0258500	AT2G28350.1	69.714	175	34	6	58	215	2	174	8.48e-71	231
Msa0258500	AT4G30080.1	63.842	177	48	6	53	215	5	179	4.19e-68	224
Msa0258500	AT1G77850.2	40.854	164	83	4	60	211	13	174	3.93e-34	130
Msa0258500	AT1G77850.1	41.104	163	82	4	61	211	14	174	4.47e-34	130
Msa0258500	AT1G30330.1	35.514	214	106	5	52	233	9	222	3.64e-32	125
Msa0258500	AT1G19220.1	42.938	177	87	6	49	211	5	181	6.99e-32	125
Msa0258500	AT5G37020.2	41.714	175	88	5	58	218	14	188	8.15e-32	125
Msa0258500	AT5G37020.1	41.714	175	88	5	58	218	14	188	1.13e-31	124
Msa0258500	AT5G20730.3	38.983	177	95	5	55	218	13	189	1.79e-31	124

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Find 55 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTCACCAGCTGCAGA+TGG	0.540159	2_2:-54844929	Msa0258500:CDS
AAAATACTTCATCAGTTTCA+GGG	0.442229	2_2:+54845981	None:intergenic
AAACTTCACCAGCTGCAGAT+GGG	0.607165	2_2:-54844928	Msa0258500:CDS
AAAGCTTAGATCCACAACTA+TGG	0.458962	2_2:-54846171	Msa0258500:CDS
AAAGTCTTTGCAAAAGATGC+AGG	0.501554	2_2:+54845875	None:intergenic
AACACTTTCGTGTTCACTGA+TGG	0.541563	2_2:+54846118	None:intergenic
AAGGAAACAATTACAGCTTT+TGG	0.228298	2_2:+54844881	None:intergenic
AATGATGATTCTGATGGTCA+TGG	0.651505	2_2:-54845917	Msa0258500:CDS
ACACAATCTGATGCAAACAA+TGG	0.698582	2_2:-54845851	Msa0258500:CDS
ACCACTCATCCTCTGTAGAG+TGG	0.584023	2_2:-54846026	Msa0258500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAGAGAGAAAAAGAAGAAA+GGG	+	chr2_2:54844888-54844907	None:intergenic	30.0%
TTTCTTTGTCTCTTATCCTT+TGG	+	chr2_2:54844925-54844944	None:intergenic	30.0%
GAAAATACTTCATCAGTTTC+AGG	+	chr2_2:54845788-54845807	None:intergenic	30.0%
CTTGAAAATGATGATTCTGA+TGG	-	chr2_2:54845842-54845861	Msa0258500:CDS	30.0%
AAGTTTAGGCATATCTATAG+AGG	-	chr2_2:54846052-54846071	Msa0258500:CDS	30.0%
CTGAAATTCTCCCTATATAT+AGG	+	chr2_2:54846337-54846356	None:intergenic	30.0%
CTTGGTTAATCAGATTATTG+CGG	-	chr2_2:54846647-54846666	Msa0258500:intron	30.0%
TGGTAATTCAGATTCGATAA+AGG	-	chr2_2:54846754-54846773	Msa0258500:intron	30.0%
TCAGAGAGAAAAAGAAGAAA+GGG	+	chr2_2:54844888-54844907	None:intergenic	30.0%
TTTCTTTGTCTCTTATCCTT+TGG	+	chr2_2:54844925-54844944	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	54844868	54846919	54844868	ID=Msa0258500;Name=Msa0258500
chr2_2	mRNA	54844868	54846919	54844868	ID=Msa0258500-mRNA-1;Parent=Msa0258500;Name=Msa0258500-mRNA-1;_AED=0.22;_eAED=0.22;_QI=119\|0.16\|0.14\|0.57\|0\|0\|7\|0\|289
chr2_2	exon	54844868	54845011	54844868	ID=Msa0258500-mRNA-1:exon:16528;Parent=Msa0258500-mRNA-1
chr2_2	exon	54845032	54845127	54845032	ID=Msa0258500-mRNA-1:exon:16527;Parent=Msa0258500-mRNA-1
chr2_2	exon	54845252	54845260	54845252	ID=Msa0258500-mRNA-1:exon:16526;Parent=Msa0258500-mRNA-1
chr2_2	exon	54845742	54845975	54845742	ID=Msa0258500-mRNA-1:exon:16525;Parent=Msa0258500-mRNA-1
chr2_2	exon	54845988	54846230	54845988	ID=Msa0258500-mRNA-1:exon:16524;Parent=Msa0258500-mRNA-1
chr2_2	exon	54846473	54846577	54846473	ID=Msa0258500-mRNA-1:exon:16523;Parent=Msa0258500-mRNA-1
chr2_2	exon	54846762	54846919	54846762	ID=Msa0258500-mRNA-1:exon:16522;Parent=Msa0258500-mRNA-1
chr2_2	five_prime_UTR	54846801	54846919	54846801	ID=Msa0258500-mRNA-1:five_prime_utr;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54846762	54846800	54846762	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54846473	54846577	54846473	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54845988	54846230	54845988	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54845742	54845975	54845742	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54845252	54845260	54845252	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54845032	54845127	54845032	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1
chr2_2	CDS	54844868	54845011	54844868	ID=Msa0258500-mRNA-1:cds;Parent=Msa0258500-mRNA-1

Gene Sequence

>Msa0258500

ATGATGAAGCTCGTGAGCCTTGTTGATGAAGTAAACAAGTTGAAGTTTCTGTCTTTCTTTTGTGGGGTTGGAAGAATCACGTTTGTGGAGCAAAAGAAGCAACCAATACACTTTTCTACCGCACTTTTTTCACTCAACTTGAAGAGTAATCAAAATTTGAAACCAATGAAAGAAGCTGAGAAAAGCTTAGATCCACAACTATGGCACGCATGTGCTGGAGGAATGGTTCAAATGCCATCAGTGAACACGAAAGTGTTTTACTTTCCACAAGGGCATGCAGAACATGCACAATCCAACGTTGATTTTGGAGATTCTTTTAGAATTCCACCACTCATCCTCTGTAGAGTGGCTTCTGTGAAATTCTTAGCTGACCCTGAAACTGATGAAATAACTTTGATTCCCTTAAGAAATTCAGAGCTTGAAAATGATGATTCTGATGGTCATGGTTCAGAGAATTCAGAAAAGCCTGCATCTTTTGCAAAGACTTTGACACAATCTGATGCAAACAATGGTGGAGGGTTCTCTGTTCCTAGATACTGTGCTGAAACGATTTTTCCTCGGCTTGATTACTCGGCTGAGCCACCGGTTCAGACGGTGATTGCAAAGGATGTTCACGGCGAGAACCGACATATTATTGCGGCCAGTTTCAAGCAAGGGGTAGCTAAAATCTTCTGCTGGAGAAAGAGAAGAAATCTGCTGGAGAATCTTCTGTTGCCTCTCAAGATGGTTATTAAAGAAGAAGCTTCTAAAATATCGGTAGAAGGACAAACGAGTCGCATTTTGCCAAAAACTTCACCAGCTGCAGATGGGAACATGAATCCAGGAAATTATATTCCAAAAGCTGTAATTGTTTCCTTTTGTTTTAGTTGA

Protein sequence

>Msa0258500

MMKLVSLVDEVNKLKFLSFFCGVGRITFVEQKKQPIHFSTALFSLNLKSNQNLKPMKEAEKSLDPQLWHACAGGMVQMPSVNTKVFYFPQGHAEHAQSNVDFGDSFRIPPLILCRVASVKFLADPETDEITLIPLRNSELENDDSDGHGSENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTVIAKDVHGENRHIIAASFKQGVAKIFCWRKRRNLLENLLLPLKMVIKEEASKISVEGQTSRILPKTSPAADGNMNPGNYIPKAVIVSFCFS*