AlfalfaGEDB | Msa0258650

M. sativa cultivar zhongmu-4 / Msa0258650

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140110 GO:1901576 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258650	XP_024631624.1	96.785	311	7	2	1	310	1	309	0.0	623

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258650	sp\|Q9SGD1\|ZWIP3_ARATH	56.845	336	100	11	8	300	3	336	2.21e-116	342

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258650	A0A396JBA6	96.785	311	7	2	1	310	1	309	0.0	623

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TFs/TRs:

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Gene ID	Type	Classification
Msa0258650	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0258650	Msa0258720	0.906373	1.589723e-80	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258650	MtrunA17_Chr2g0321291	96.785	311	7	2	1	310	1	309	0.0	623
Msa0258650	MtrunA17_Chr4g0026521	66.124	307	82	6	6	308	5	293	4.44e-128	367
Msa0258650	MtrunA17_Chr7g0258011	62.195	246	80	4	73	306	103	347	5.71e-109	320
Msa0258650	MtrunA17_Chr1g0173841	60.643	249	75	5	78	306	141	386	7.14e-106	314
Msa0258650	MtrunA17_Chr8g0352011	64.444	225	63	3	82	296	110	327	1.04e-103	306
Msa0258650	MtrunA17_Chr2g0328921	63.636	220	68	3	69	282	73	286	1.70e-101	300
Msa0258650	MtrunA17_Chr5g0422571	63.679	212	62	4	74	284	55	252	1.47e-97	289
Msa0258650	MtrunA17_Chr4g0053341	57.724	246	87	2	46	290	1	230	5.23e-95	280
Msa0258650	MtrunA17_Chr1g0198651	77.707	157	32	1	131	284	99	255	5.52e-94	280
Msa0258650	MtrunA17_Chr1g0200421	39.873	158	81	7	141	289	245	397	8.81e-27	109

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0258650	AT1G08290.1	56.845	336	100	11	8	300	3	336	2.25e-117	342
Msa0258650	AT1G51220.1	51.757	313	107	6	19	306	43	336	1.47e-103	306
Msa0258650	AT3G20880.1	63.158	228	67	2	84	294	176	403	3.94e-101	303
Msa0258650	AT3G57670.1	59.184	245	67	4	84	296	122	365	1.38e-100	300
Msa0258650	AT1G13290.1	65.347	202	52	4	84	283	54	239	2.75e-93	279
Msa0258650	AT1G34790.1	60.090	223	80	3	84	303	83	299	6.50e-93	278
Msa0258650	AT1G34370.3	38.608	158	85	5	141	289	28	182	7.84e-28	109
Msa0258650	AT1G34370.2	38.608	158	85	5	141	289	238	392	2.82e-27	111
Msa0258650	AT1G34370.1	38.608	158	85	5	141	289	238	392	2.82e-27	111
Msa0258650	AT5G22890.1	39.735	151	83	5	141	284	211	360	1.79e-24	102

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Find 60 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCCAAATTTCATTGAA+TGG	0.361241	2_2:+55127670	Msa0258650:CDS
AAAGATCATGTTAGATCCTT+CGG	0.408333	2_2:+55130756	Msa0258650:CDS
AAAGGACCAGACTCACTTAA+AGG	0.304674	2_2:+55130465	Msa0258650:CDS
AAGCCATCAAGAGAAGGAAG+TGG	0.595192	2_2:-55130792	None:intergenic
AAGCCCTTTGAGGACGACCA+TGG	0.585506	2_2:+55127801	Msa0258650:CDS
ACAAGAATCTGAGCAGGAGT+TGG	0.574558	2_2:-55128023	None:intergenic
ACTCATTACAAAAGAAAGCA+TGG	0.557148	2_2:+55130600	Msa0258650:CDS
ACTCCTGCTCAGATTCTTGT+TGG	0.408478	2_2:+55128026	Msa0258650:CDS
AGAGTTCTGAAATCCTTCAA+TGG	0.491722	2_2:-55130573	None:intergenic
AGCCATCAAGAGAAGGAAGT+GGG	0.568332	2_2:-55130791	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACCCCAAATTTCATTGAA+TGG	+	chr2_2:55127670-55127689	Msa0258650:CDS	30.0%
TGAGCCATTCAATGAAATTT+GGG	-	chr2_2:55127677-55127696	None:intergenic	30.0%
TTGAGCCATTCAATGAAATT+TGG	-	chr2_2:55127678-55127697	None:intergenic	30.0%
GTAAAAACTGGAAAGTTTCT+TGG	-	chr2_2:55127764-55127783	None:intergenic	30.0%
TGAACATGATGAGAAGAATA+AGG	+	chr2_2:55127920-55127939	Msa0258650:CDS	30.0%
CAATAGATACAACAATATGC+AGG	+	chr2_2:55128088-55128107	Msa0258650:CDS	30.0%
AATTTCCCCTAAAAATCGAA+GGG	-	chr2_2:55128163-55128182	None:intergenic	30.0%
AAATTTCCCCTAAAAATCGA+AGG	-	chr2_2:55128164-55128183	None:intergenic	30.0%
TAAAAATCGAAGGGTTAAGA+AGG	-	chr2_2:55128154-55128173	None:intergenic	30.0%
TTAGGTCAATAATTTCCCTT+AGG	+	chr2_2:55128327-55128346	Msa0258650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	55127566	55131080	55127566	ID=Msa0258650;Name=Msa0258650
chr2_2	mRNA	55127566	55131080	55127566	ID=Msa0258650-mRNA-1;Parent=Msa0258650;Name=Msa0258650-mRNA-1;_AED=0.01;_eAED=0.01;_QI=55\|1\|1\|1\|1\|1\|2\|205\|310
chr2_2	exon	55127566	55128109	55127566	ID=Msa0258650-mRNA-1:exon:16654;Parent=Msa0258650-mRNA-1
chr2_2	exon	55130432	55131080	55130432	ID=Msa0258650-mRNA-1:exon:16655;Parent=Msa0258650-mRNA-1
chr2_2	five_prime_UTR	55127566	55127620	55127566	ID=Msa0258650-mRNA-1:five_prime_utr;Parent=Msa0258650-mRNA-1
chr2_2	CDS	55127621	55128109	55127621	ID=Msa0258650-mRNA-1:cds;Parent=Msa0258650-mRNA-1
chr2_2	CDS	55130432	55130875	55130432	ID=Msa0258650-mRNA-1:cds;Parent=Msa0258650-mRNA-1
chr2_2	three_prime_UTR	55130876	55131080	55130876	ID=Msa0258650-mRNA-1:three_prime_utr;Parent=Msa0258650-mRNA-1

Gene Sequence

>Msa0258650

ATGGGTTTGAGGGATCAGTGCAATGTAGAAGACAAGAATCTCTACCTTCAAACCCCAAATTTCATTGAATGGCTCAAACCACCTAATTCCTCACTCTCATCATCTCCTTCATCGCCTTTAACTCATCAACACAAACTATCCCAAGAAACTTTCCAGTTTTTACCAATCTCTAGTGGAGTTAAGCCCTTTGAGGACGACCATGGGATGCAAAAGGAAGATTTTGAAGTAAAAGAAGAGAAGGTGGAACATGTGACTGTGGCTTTGCACATTGGATTGCCTAACGTAGGATGTGAATCTGATGAACATGATGAGAAGAATAAGGTTTTTGATTGTGTTAAGGAAGAAGAATTGAAGAAAAACGTGCATGGTTTTTGTTTTAAGGAGGAGAGAAGGTTTTGGATACCAACTCCTGCTCAGATTCTTGTTGGACCTATGCAGTTTGCTTGCTCCATATGCAGTAAGACCTTCAATAGATACAACAATATGCAGATGCATATGTGGGGGCATGGATCAGAATTTAGGAAAGGACCAGACTCACTTAAAGGAACACAACCAGCAGCAATGCTAAGGCTGCCATGTTATTGTTGTGCTCACGGTTGCAAGAACAACATCAATCATCCAAGAGCAAAACCATTGAAGGATTTCAGAACTCTCCAAACTCATTACAAAAGAAAGCATGGAACAAAACCATTTATCTGCAGAAAATGTTCTAAAGCATTTGCTGTTAAAGGTGATTGGAGAACTCATGAGAAAAATTGTGGAAAGTTTTGGTATTGCACCTGTGGTTCAGATTTTAAACACAAAAGATCTCTTAAAGATCATGTTAGATCCTTCGGAAAAGGTCATTCCCCACTTCCTTCTCTTGATGGCTTTGAAGATGAGAAAGAATGCAGCAATACTGGATCTGATGATGATGAAGTTGCTCATGCATGA

Protein sequence

>Msa0258650

MGLRDQCNVEDKNLYLQTPNFIEWLKPPNSSLSSSPSSPLTHQHKLSQETFQFLPISSGVKPFEDDHGMQKEDFEVKEEKVEHVTVALHIGLPNVGCESDEHDEKNKVFDCVKEEELKKNVHGFCFKEERRFWIPTPAQILVGPMQFACSICSKTFNRYNNMQMHMWGHGSEFRKGPDSLKGTQPAAMLRLPCYCCAHGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFICRKCSKAFAVKGDWRTHEKNCGKFWYCTCGSDFKHKRSLKDHVRSFGKGHSPLPSLDGFEDEKECSNTGSDDDEVAHA*